WO2004014864A1 - 1h-indazole-3-carboxamide compounds as cyclin dependent kinases (cdk) inhibitors - Google Patents

1h-indazole-3-carboxamide compounds as cyclin dependent kinases (cdk) inhibitors Download PDF

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WO2004014864A1
WO2004014864A1 PCT/GB2003/003491 GB0303491W WO2004014864A1 WO 2004014864 A1 WO2004014864 A1 WO 2004014864A1 GB 0303491 W GB0303491 W GB 0303491W WO 2004014864 A1 WO2004014864 A1 WO 2004014864A1
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group
indazole
carboxylic acid
hydrogen
compound
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PCT/GB2003/003491
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French (fr)
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Valerio Berdini
Alessandro Padova
Gordon Saxty
Alison Jo-Anne Woolford
Paul Graham Wyatt
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Astex Technology Limited
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Priority to EP03784286A priority Critical patent/EP1534685A1/en
Priority to US10/524,784 priority patent/US20060135589A1/en
Priority to AU2003255779A priority patent/AU2003255779A1/en
Priority to JP2004527053A priority patent/JP2006500348A/en
Publication of WO2004014864A1 publication Critical patent/WO2004014864A1/en

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    • C07D231/00—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings
    • C07D231/54—Heterocyclic compounds containing 1,2-diazole or hydrogenated 1,2-diazole rings condensed with carbocyclic rings or ring systems
    • C07D231/56—Benzopyrazoles; Hydrogenated benzopyrazoles
    • A—HUMAN NECESSITIES
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    • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
    • A—HUMAN NECESSITIES
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    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
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    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D403/12—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, not provided for by group C07D401/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/04—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D405/00—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom
    • C07D405/02—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings
    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D409/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms
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    • C07D409/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having sulfur atoms as the only ring hetero atoms containing two hetero rings directly linked by a ring-member-to-ring-member bond
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    • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings directly linked by a ring-member-to-ring-member bond
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    • C07—ORGANIC CHEMISTRY
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    • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
    • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/04—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings directly linked by a ring-member-to-ring-member bond
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    • C07D—HETEROCYCLIC COMPOUNDS
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    • C07D417/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings
    • C07D417/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
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    • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings

Definitions

  • This invention relates to 3 -substituted indazole compounds that inhibit or modulate the activity of cyclin dependent kinases (CDK), to the use ofthe compounds in the treatment or prophylaxis of disease states or conditions mediated by cyclin dependent kinases, and to novel compounds having cyclin dependent kinase inhibitory or modulating activity. Also provided are pharmaceutical compositions containing the compounds and novel chemical intermediates.
  • CDK cyclin dependent kinases
  • Protein kinases constitute a large family of structurally related enzymes that are responsible for the control of a wide variety of signal transduction processes within the cell (Hardie, G. and Hanks, S. (1995) The Protein Kinase Facts Book. I and II, Academic Press, San Diego, CA).
  • the kinases may be categorized into families by the substrates they phosphorylate (e.g., protein-tyrosine, protein-serine/threonine, lipids, etc.).
  • Protein kinases may be characterized by their regulation mechanisms. These mechanisms include, for example, autophosphorylation, transphosphorylation by other kinases, protein-protein interactions, protein-lipid interactions, and protein- polynucleotide interactions. An individual protein kinase may be regulated by more than one mechanism.
  • Kinases regulate many different cell processes including, but not limited to, proliferation, differentiation, apoptosis, motility, transcription, translation and other signalling processes, by adding phosphate groups to target proteins. These phosphorylation events act as molecular on off switches that can modulate or regulate the target protein biological function. Phosphorylation of target proteins occurs in response to a variety of extracellular signals (hormones, neurotransmitters, growth and differentiation factors, etc.), cell cycle events, environmental or nutritional stresses, etc.
  • the appropriate protein kinase functions in signalling pathways to activate or inactivate (either directly or indirectly), for example, a metabolic enzyme, regulatory protein, receptor, cytoskeletal protein, ion channel or pump, or transcription factor.
  • Uncontrolled signalling due to defective control of protein phosphorylation has been implicated in a number of diseases, including, for example, inflammation, cancer, allergy/asthma, disease and conditions ofthe immune system, disease and conditions ofthe central nervous system, and angiogenesis.
  • CDKs cyclin dependent kinases
  • cyclins are cdc2 (also known as CDK1) homologous serine-threonine kinase proteins that are able to utilise ATP as a substrate in the phosphorylation of diverse polypeptides in a sequence dependent context.
  • Cyclins are a family of proteins characterised by a homology region, containing approximately 100 amino acids, termed the "cyclin box" which is used in binding to, and defining selectivity for, specific CDK partner proteins.
  • Modulation ofthe expression levels, degradation rates, and activation levels of various CDKs and cyclins throughout the cell cycle leads to the cyclical formation of a series of CDK/cyclin complexes, in which the CDKs are enzymatically active.
  • the formation of these complexes controls passage through discrete cell cycle checkpoints and thereby enables the process of cell division to continue.
  • Failure to satisfy the pre-requisite biochemical criteria at a given cell cycle checkpoint, i.e. failure to form a required CDK/cyclin complex can lead to cell cycle arrest and/or cellular apoptosis. Aberrant cellular proliferation, as manifested in cancer, can often be attributed to loss of correct cell cycle control.
  • Progression from the Gl phase to the S phase ofthe cell cycle is primarily regulated by CDK2, CDK3, CDK4 and CDK6 via association with members ofthe D and E type cyclins.
  • the D-type cyclins appear instrumental in enabling passage beyond the Gl restriction point, where as the CDK2/cyclin E complex is key to the transition from the Gl to S phase. Subsequent progression through S phase and entry into G2 is thought to require the CDK2/cyclin A complex.
  • Both mitosis, and the G2 to M phase transition which triggers it are regulated by complexes of CDK 1 and the A and B type cyclins.
  • Rb Retinoblastoma protein
  • Rb and related pocket proteins are substrates for CDK(2, 4, & 6)/cyclin complexes. Progression through Gl is in part facilitated by hyperphosphorylation, and thus inactivation, of Rb and pi 30 by the CDK(4/6)/cyclin-D complexes. Hyperphosphorylation of Rb and pl30 causes the release of transcription factors, such as E2F, and thus the expression of genes necessary for progression through Gl and for entry into S-phase, such as the gene for cyclin E. Expression of cyclin E facilitates formation ofthe CDK2/cyclin E complex which amplifies, or maintains, E2F levels via further phosphorylation of Rb.
  • transcription factors such as E2F
  • CDK3 has a role in regulating the Gl/S transition.
  • CDK5 which is necessary for correct neuronal development and which has also been implicated in the phosphorylation of several neuronal proteins such as Tau, NUDE-1, synapsinl, DARPP32 and the
  • CDK7 is a nuclear protein that has cdc2 CAK activity and binds to cyclin H.
  • CDK7 has been identified as component ofthe TFIIH transcriptional complex which has RNA polymerase II C-terminal domain (CTD) activity. This has been associated with the regulation of HIN-1 transcription via a Tat-mediated biochemical pathway.
  • CTD RNA polymerase II C-terminal domain
  • CDK8 binds cyclin C and has been implicated in the phosphorylation ofthe CTD of R ⁇ A polymerase II.
  • the CDK9/cyclin-Tl complex (P-TEFb complex) has been implicated in elongation control of R ⁇ A polymerase II.
  • PTEF-b is also required for activation of transcription ofthe HIN-1 genome by the viral transactivator Tat through its interaction with cyclin Tl .
  • CDK7, CDK8, CDK9 and the P-TEFb complex are therefore potential targets for anti-viral therapeutics.
  • CDK phosphorylation is performed by a group of CDK activating kinases (CAKs) and/or kinases such as weel, Mytl and Mikl.
  • Dephosphorylation is performed by phosphatases such as cdc25(a & c), pp2a, or KAP.
  • CDK/cyclin complex activity may be further regulated by two families of endogenous cellular proteinaceous inhibitors: the Kip/Cip family, or the INK family.
  • the INK proteins specifically bind CDK4 and CDK6.
  • ⁇ l6 Ink4 also known as MTS1
  • MTS1 is a potential tumour suppressor gene that is mutated, or deleted, in a large number of primary cancers.
  • the Kip/Cip family contains proteins such as p21 c ipi, w afi . p27 ⁇ ipl and p57 ⁇ i 2 .
  • p21 is induced by p53 and is able to inactivate the CDK2/cyclin(E/A) and CDK4/cyclin(Dl/D2/D3) complexes.
  • Atypically low levels of p27 expression have been observed in breast, colon and prostate cancers.
  • Conversely over expression of cyclin E in solid tumours has been shown to correlate with poor patient prognosis.
  • Over expression of cyclin Dl has been associated with oesophageal, breast, squamous, and non- small cell lung carcinomas.
  • CDKs The pivotal roles of CDKs, and their associated proteins, in co-ordinating and driving the cell cycle in proliferating cells have been outlined above. Some ofthe biochemical pathways in which CDKs play a key role have also been described.
  • CDK inhibitors could conceivably also be used to treat other conditions such as viral infections, autoimmune diseases and neuro-degenerative diseases, amongst others.
  • CDK targeted therapeutics may also provide clinical benefits in the treatment ofthe previously described diseases when used in combination therapy with either existing, or new, therapeutic agents.
  • CDK targeted anticancer therapies could potentially have advantages over many current antitumour agents as they would not directly interact with DNA and should therefore reduce the risk of secondary tumour development.
  • WO 02/34721 from Du Pont discloses a class of indeno [l,2-c]pyrazol-4-ones as inhibitors of cyclin dependent kinases.
  • WO 01/72745 Al from Cyclacel describes 2-substituted 4-heteroaryl-pyrimidines and their preparation, pharmaceutical compositions containing them and their use as inhibitors of cyclin-dependant kinases (CDKs) and hence their use in the treatment of proliferative disorders such as cancer, leukaemia, psoriasis and the like.
  • CDKs cyclin-dependant kinases
  • WO 99/21845 from Agouron describes 4-aminothiazole derivatives for inhibiting cyclin-dependent kinases (CDKs), such as CDK1, CDK2, CDK4, and CDK6.
  • CDKs cyclin-dependent kinases
  • the invention is also directed to the therapeutic or prophylactic use of pharmaceutical compositions containing such compounds and to methods of treating malignancies and other disorders by administering effective amounts of such compounds.
  • WO 01/53274 from Agouron discloses as CDK kinase inhibitors a class of compounds which can comprise an amide-substituted benzene ring linked to an N- containing heterocyclic group.
  • indazole compounds are not mentioned generically, one ofthe exemplified compounds comprises an indazole 3 -carboxylic acid anilide moiety linked via a methylsulphanyl group to a pyrazolopyrimidme.
  • WO 01/98290 discloses a class of 3-aminocarbonyl-2- carboxamido thiophene derivatives as protein kinase inhibitors. The compounds are stated to have multiple protein kinase activity.
  • GB 1301882 US 3,705,175, DE 2,135,398 (all to Egyt), and Ferenc et al, Magyar Kemikusok Lapja, 1975, 30(4), 208-215, each disclose 6,7-dimethoxyindazole-3- carboxylic acid amides as anti-inflammatory and analgesic agents.
  • US 3,457,269 and US 3,145,215 both to Sterling Drug disclose indazole-3- carboxylic acid amides, including anilides, cycloaliphatic amides and pyridylamides, as hypotensive agents.
  • WO 01/53268 and WO 01/02369 from Agouron disclose compounds that mediate or inhibit cell proliferation through the inhibition of protein kinases such as cyclin dependent kinase or tyrosine kinase.
  • WO 02/10137 discloses a class of indazole derivatives as selective inhibitors of JNK kinase.
  • the indazole derivatives have an aryl, heteroaryl or heterocyclic group linked to the indazole 3-position through an akylene or alkenylene group.
  • US 6,340,685 discloses a class of bicyclic heterocyclic compounds as selective P38 MAP kinase inhibitors. Indazoles are not specifically disclosed.
  • WO 02/24635 discloses a class of amino alcohol derivatives as ⁇ -3 adrenergic receptor agonists.
  • the compounds can contain an indazole 3- carboxylic acid anilide group linked to the amino alcohol group.
  • JP 04089489 (Nisshin), JP 03223280 (Dainippon), JP 05230057 (Dainippon), JP 04005289 (Hokuriku), JP 06135960 (Dainippon), EP 0499995 (Nisshin), EP 0623621 (Nisshin), WO 96/38420 (Nisshin), EP 0708105 (Nisshin), EP 0358903 (Dainippon), Harada et al. Chem. Pharm. Bull, 43 (11), 1912-1930 (1995), Harada et al. Chem. Pharm. Bull, 44 (12), 2205-2212 (1996) and Morie et al. Synthetic Communications, 27(4), 559-566 (1997) each disclose indazole 3-carboxamides in which the amide nitrogen is linked to a non-aromatic cyclic amino group. The compounds are described as being active as 5-HT receptor modulators.
  • EP 0410509 discloses, as 5-HT receptor antagonists, a class of indazole 3- carboxamides in which the amide nitrogen is linked to an imidazolylmethyl group.
  • Indazole carboxylic acid derivatives are also disclosed as 5-HT receptor modulators in WO 93/03725 (SmithKline Beecham), EP 0261964 (Beecham), EP 0517984 (Merrell Dow), US 5,654,320 (Eli Lilly), EP 0908452 (Eli Lilly), EP 0908459 (Eli Lilly) and EP 0732333 (Eli Lilly).
  • WO 01/58869 (Bristol Myers Squibb) discloses a number of indazole-3- carboxamide derivatives as cannabinoid receptor antagonists.
  • WO 02/20484 (Astra Zeneca) discloses a broad class of compounds, including compounds containing an indazole group, as modulators of chemokine receptor activity. No indazoles are exemplified however. .
  • WO 02/053534 discloses a class of carboxylic acids and their esters as NLA inhibitors.
  • the compounds which are stated to be useful in the treatment of various disease states including inflammatory conditions, can comprise a halogenated phenyl acetic acid moiety linked to an indazole-3-carboxamido group.
  • WO 93/01169 describes a class of compounds that have tachykinin receptor antagonist activity.
  • the compounds may contain an indazole group, but there are no examples of indazole-3-carboxamides.
  • WO 98/03494 discloses a class of 1 -phenyl- 1-piperazino-cycloalkanes and aza-cycloalkanes in which the phenyl group can form part of an indazole-3- carboxylic acid phenylamide.
  • the compounds are disclosed as being capable of binding to mammalian neuropeptide Yl.
  • WO 99/29661 (Astra) describes a broad class of adamantane derivatives and oxa- adamantane derivatives as being useful in the treatment of rheumatoid arthritis, osteoarthritis, psoriasis and the growth and metastasis of malignant cells.
  • indazoles there are no examples of indazoles.
  • WO 01/57024 discloses the use of various compounds, including indazoles, for blocking voltage dependent sodium channels.
  • WO 01/83472 (Acadia) describes a class of bicyclic heteroaryl compounds as muscarinic agonists.
  • One ofthe exemplified compounds is the l-butyl-4- piperidinomethyl amide of indazole-3 -carboxylic acid.
  • EP 01013276 discloses a class of compounds as modulators of chemokine activity that can be used in the treatment of inflammatory conditions. Indazoles are amongst the large list of compounds mentioned but there are no examples of indazoles.
  • WO 02/16318 discloses vanilloid receptor modulators for the treatment of inflammatory diseases.
  • the modulator compounds can be indazoles but there is no disclosure of indazole-3-carboxamides.
  • WO 99/49856 discloses compounds that are useful in treating CD11/CDl 8 adhesion receptor mediated disorders such as inflammation, psoriasis and rheumatoid artliritis.
  • the compounds can contain an indazole unit but there are no examples of indazole-3 -carboxamides.
  • JP 01117882 discloses heteroarylamides for use in treating certain disorders ofthe gastrointestinal system.
  • JP 11130750 discloses a class of arylamides for use in the treatment of CNS disorders.
  • WO 00/18738 discloses a class of bis-amidophenyl compounds that act as inhibitors of cytokine production and which are stated to be useful in the treatment of inflammatory and allergic disease states.
  • the compounds can contain an indazole unit but there are no examples of indazoles.
  • the invention provides compounds that have cyclin dependent kinase inhibiting or modulating activity, and which it is envisaged will be useful in preventing or treating disease states or conditions mediated by the cyclin dependent kinases. Accordingly, in one aspect, the invention provides a compound ofthe formula (I) as defined herein for use in the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
  • the invention also provides the use of a compound ofthe formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
  • This invention also provides a method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, which method comprises administering to the mammal a compound ofthe formula (I) as defined herein in an amount effective in inhibiting abnormal cell growth.
  • This invention further provides a method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, the method comprising administering to the mammal a compound ofthe formula (I) as defined herein in an amount effective to inhibit CDK2 activity.
  • the invention provides a method of inhibiting a cyclin dependent kinase, which method comprises contacting the kinase with a kinase-inhibiting compound ofthe formula (I) as defined herein.
  • the invention further provides a method of modulating a cellular process (for example cell division) by inhibiting the activity of a cyclin dependent kinase using a compound ofthe formula (I) as defined herein.
  • A is a group R 2 or CH -R 2 where R 2 is a carbocyclic or heterocyclic group having from 3 to 12 ring members;
  • B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
  • R 1 is hydrogen or a group selected from SO 2 R b , SO 2 NR 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
  • R 3 , R 4 , R 5 and R 6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a - R b wherein R a is a bond, O, CO, X J C(X 2 ), C(X 2 )X 1 , X ⁇ CX ⁇ X 1 , S, SO, SO 2 , NR C , SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Ci_ hydrocarbyl group optionally substituted by one or more
  • R c is hydrogen or C ⁇ hydrocarbyl
  • R 7 is selected from hydrogen and a C ⁇ s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C ⁇ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the C ⁇ -8 hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X 1 C(X 2 ), C(X 2 )X l or X 1 C(X 2 )X 1 ;
  • R 8 is selected from R 7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members
  • R 9 is selected from R 8 , COR 8 and SO 2 R 8
  • NR 7 R 8 or NR 7 R 9 may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N-[(morpholin-4-yl)phenyl-lH-indazole-3- carboxamide and N-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide.
  • the group A is a group R or CH 2 -R where R is a carbocyclic or heterocyclic group having from 3 to 12 ring members. In one particular embodiment, A is a group R 2 .
  • the term “carbocyclic and heterocyclic groups having from 3 to 12 ring members” includes within its scope aromatic, non-aromatic, unsaturated, partially saturated and fully saturated carbocyclic and heterocyclic ring systems.
  • the carbocyclic or heterocyclic groups can be aryl or heteroaryl groups having from 5 to 12 ring members, more usually from 5 to 10 ring members.
  • aryl refers to a carbocyclic group having aromatic character and the term “heteroaryl” is used herein to denote a heterocyclic group having aromatic character.
  • aryl and heteroaryl embrace polycyclic (e.g. bicyclic) ring systems wherein one or more rings are non-aromatic, provided that at least one ring is aromatic.
  • the aryl or heteroaryl groups can be monocyclic or bicyclic groups and can be unsubstituted or substituted with one or more substituents, for example one or more groups R 10 as defined below.
  • heteroaryl groups are monocyclic and bicyclic groups containing from five to twelve ring members, and more usually from five to ten ring members.
  • the heteroaryl group can be, for example, a five membered or six membered monocyclic ring or a bicyclic structure formed from fused five and six membered rings or two fused six membered rings.
  • Each ring may contain up to about four heteroatoms typically selected from nitrogen, sulphur and oxygen.
  • the heteroaryl ring will contain up to 3 heteroatoms, more usually up to 2, for example a single heteroatom.
  • the heteroaryl ring contains at least one ring nitrogen atom.
  • the nitrogen atoms in the heteroaryl rings can be basic, as in the case of a pyrazole, imidazole or pyridine, or essentially non-basic as in the case of an indole or pyrrole nitrogen.
  • the number of basic nitrogen atoms present in the heteroaryl group, including any amino group substituents ofthe ring, will be less than five.
  • heteroaryl groups include but are not limited to pyridyl, pyrrolyl, furanyl, thiophenyl, imidazolyl, oxazolyl, oxadiazolyl, oxatriazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, triazolyl, tetrazolyl, quinolinyl, isoquinolinyl, benzfuranyl, benzthiophenyl, chromanyl, thiochromanyl, benzimidazolyl, benzoxazolyl, benzisoxazole, benzthiazolyl and benzisothiazole, isobenzofuranyl, isoindolyl, indolizinyl, indolinyl, isoindolinyl, purinyl
  • one particular sub-group of compounds ofthe formula (I) is the group wherein R 2 is selected from pyridyl, quinolinyl, isoquinolinyl and thiadiazolyl.
  • the pyridyl group can be a 2-pyridyl, 3-pyridyl or 4-pyridyl group but preferably it is a 3-pyridyl group.
  • carbocyclic aryl groups include phenyl, naphthyl, indenyl, and tetrahydronaphthyl.
  • preferred aryl groups are groups based on a benzene ring. Thus it may be, for example, a phenyl group which has no substituents other than the group B, or has one or more further substituents R 10 as defined herein.
  • non-aromatic heterocyclic groups are groups having from 3 to 12 ring members, more usually 5 to 10 ring members. Such groups can be monocyclic or bicyclic, for example, and typically have from 1 to 5 heteroatom ring members (more usually 1, 2, 3 or 4 heteroatom ring members), usually selected from nitrogen, oxygen and sulphur.
  • the heterocylic groups can contain, for example, cyclic ether moieties (e.g as in tetrahydrofuran and dioxane), cyclic thioether moieties (e.g. as in tetrahydrothiophene), cyclic amine moieties (e.g.
  • cyclic amides such as a pyrrolidinone, piperidone or caprolactam
  • cyclic sulphonamides such as an isothiazolidine 1,1 -dioxide, [l,2]thiazinane 1,1- dioxide or [l,2]thiazepane 1,1 -dioxide
  • cyclic sulphones e.g. as in sulpholane and sulpholene
  • cyclic sulphoxides and combinations thereof.
  • Particular examples include morpholine, piperidine, pyrrolidine, pyrrolidone, tetrahydrofuran, tetrahydrothiophene, dioxan, tetrahydropyran, imidazoline, imidazolidinone, oxazoline, thiazoline, piperazine, and N-alkyl piperazines such as N-methyl piperazine.
  • preferred non-aromatic heterocyclic groups include tetrahydrofuran, morpholine, piperazine, piperidine, pyrrolidine and pyrrolidone.
  • the carbocyclic and heterocyclic groups can be polycyclic fused ring systems but it is preferred that they are not bridged ring systems such as bicycloalkanes, tricycloalkanes and their oxa- and aza analogues (e.g. adamantane and oxa- adamantane).
  • bridged ring systems such as bicycloalkanes, tricycloalkanes and their oxa- and aza analogues (e.g. adamantane and oxa- adamantane).
  • the group R 10 is selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a -R b wherein R a is a bond, O, CO, X ⁇ CX ⁇ X 1 , S, SO, SO 2 , NR C , SO 2 NR° or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C 1-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C ⁇ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C ⁇ s hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR°
  • substituent group R 10 comprises or includes a carbocyclic or heterocyclic group
  • the said carbocyclic or heterocyclic group may be unsubstituted or may itself be substituted with one or more further substituent groups R 10 .
  • such further substituent groups R may include carbocyclic or heterocyclic groups.
  • the said further substituents do not include carbocyclic or heterocyclic groups but are otherwise selected from the groups listed above in the definition of R 10 .
  • the substituent groups R 10 may be selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, amino; a group R -R wherein R is a bond, O, CO, X ⁇ C(X 2 ), S, SO, SO 2 , NR C , SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen and a Ci-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C 1 .
  • halogen substituents include fluorine, chlorine, bromine and iodine. Fluorine and chlorine are particularly preferred.
  • hydrocarbyl is a generic term encompassing aliphatic, alicyclic and aromatic groups having an all-carbon backbone, except where otherwise stated.
  • examples of such groups include alkyl, cycloalkyl, cycloalkenyl, carbocyclic aryl, alkenyl, alkynyl, cycloalkylalkyl, cycloalkenylalkyl, and carbocyclic aralkyl, aralkenyl and aralkynyl groups.
  • Such groups can be unsubstituted or substituted by one or more substituents as defined herein.
  • the examples and preferences expressed below apply to each ofthe hydrocarbyl substituent groups or hydrocarbyl-containing substituent groups referred to in the various definitions of substituents for compounds ofthe formula (I) unless the context indicates otherwise.
  • the hydrocarbyl groups can have up to eight carbon atoms, unless the context requires otherwise.
  • C ⁇ _ 6 hydrocarbyl groups such as CM hydrocarbyl groups (e.g. C 1-3 hydrocarbyl groups or d. 2 hydrocarbyl groups), specific examples being any individual value or combination of values selected from , C 2 , C 3 , C , C 5 , C 6 , C and C 8 hydrocarbyl groups.
  • alkyl covers both straight chain and branched chain alkyl groups.
  • alkyl groups include methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, 2-pentyl, 3-pentyl, 2-methyl butyl, 3-methyl butyl, and n-he ⁇ yl and its isomers.
  • C ⁇ alkyl groups such as C M alkyl groups (e.g. C 1 . 3 alkyl groups or C1-2 alkyl groups).
  • cycloalkyl groups are those derived from cyclopropane, cyclobutane, cyclopentane, cyclohexane and cycloheptane. Within the sub-set of cycloalkyl groups the cycloalkyl group will have from 3 to 8 carbon atoms, particular examples being C 3 _ 6 cycloalkyl groups.
  • alkenyl groups include, but are not limited to, ethenyl (vinyl), 1- propenyl, 2-propenyl (allyl), isopropenyl, butenyl, buta-l,4-dienyl, pentenyl, and hexenyl.
  • alkenyl groups will have 2 to 8 carbon atoms, particular examples being C 2 -6 alkenyl groups, such as C 2 - 4 alkenyl groups.
  • cycloalkenyl groups include, but are not limited to, cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclopentadienyl and cyclohexenyl. Within the sub- set of cycloalkenyl groups the cycloalkenyl groups have from 3 to 8 carbon atoms, and particular examples are C 3 .6 cycloalkenyl groups.
  • alkynyl groups include, but are not limited to, ethynyl and 2-propynyl (propargyl) groups. Within the sub-set of alkynyl groups having 2 to 8 carbon atoms, particular examples are C 2 -6 alkynyl groups, such as C 2 . 4 alkynyl groups.
  • carbocyclic aryl groups include substituted and unsubstituted phenyl.
  • cycloalkylalkyl, cycloalkenylalkyl, carbocyclic aralkyl, aralkenyl and aralkynyl groups include phenethyl, benzyl, styryl, phenylethynyl, cyclohexylmethyl, cyclopentylmethyl, cyclobutylmethyl, cyclopropylmethyl and cyclopentenylmethyl groups.
  • a hydrocarbyl group can be optionally substituted by one or more substituents selected from hydroxy, oxo, alkoxy, carboxy, halogen, cyano, nitro, amino, mono- or di-C ⁇ hydrocarbylamino, and monocyclic or bicyclic carbocyclic and heterocyclic groups having from 3 to 12 (typically 3 to 10 and more usually 5 to 10) ring members.
  • Preferred substituents include halogen such as fluorine.
  • the substituent can be a partially fluorinated or perfluorinated group such as trifluoromethyl.
  • preferred substituents include monocyclic carbocyclic and heterocyclic groups having 3-7 ring members.
  • One or more carbon atoms of a hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X 1 C(X 2 ), C(X 2 )X l or X 1 C(X 2 )X 1 wherein X 1 and X 2 are as hereinbefore defined.
  • 1, 2, 3 or 4 carbon atoms ofthe hydrocarbyl group may be replaced by one ofthe atoms or groups listed, and the replacing atoms or groups may be the same or different.
  • Examples of groups in which a carbon atom ofthe hydrocarbyl group has been replaced by a replacement atom or group as defined above include ethers and thioethers (C replaced by O or S), amides, esters, thioamides and thioesters (C replaced by X ! C(X 2 ) or C(X 2 )X 1 ), sulphones and sulphoxides (C replaced by SO or SO 2 ) and amines (C replaced by NR C ).
  • an amino group may, together with the nitrogen atom to which they are attached, and optionally with another heteroatom such as nitrogen, sulphur, or oxygen, link to form a ring structure of 4 to 7 ring members.
  • R a -R b as used herein, either with regard to substituents present on the carbocyclic or heterocyclic moiety R 2 , or with regard to other substituents present at other locations on the compounds ofthe formula (I), includes inter alia compounds wherein R a is selected from a bond, O, CO, OC(O), SC(O), NR°C(O), OC(S), SC(S), NR C C(S), OC(NR c ), SC(NR C ), NR C C(NR°), C(O)O, C(O)S, C(O)NR°, C(S)O, C(S)S, C(S) NR C , C(NR c )O, C(NR°)S, C(NR C )NR°, OC(O)O, SC(O)O, NR c C(O)O, OC(S)O, SC(O)O, NR c C(O)O, OC(S)O, SC(O
  • hydrocarbyl, carbocyclic and heterocyclic groups are as set out above.
  • each substituent group R 10 when present, is other than a carboxy group or a hydrocarbyl group terminated by a carboxy group or alkoxycarbonyl group.
  • B is a bond or an acyclic linker group.
  • the linker group has a linking chain length of up to 3 atoms: in other words the number of atoms in the backbone ofthe linker group is 1, 2 or 3.
  • a group -CH 2 - has a linking chain length of one
  • a group -CH 2 -CH 2 - has a linking chain length of two.
  • the atoms making up the backbone ofthe linker group are selected from C, N, S and O, but preferably the atoms defining the linking chain length are all carbon atoms.
  • the linker group is typically a straight chain group.
  • straight chain is meant a group that has no branched side chains.
  • a straight chain linker group may bear single atom substituents such as halogen and oxo, or substituents each of 1, 2 or 3 atoms, but would not usually have hydrocarbon substituents such as methyl, or larger multi-atom substituents each having four atoms or more such as methoxy or trifluoromethyl for example.
  • a preferred linker group B is a group (CH 2 ) n wherein n is 1, 2 or 3, more preferably 1 or 2, and most preferably 1.
  • the groups R 3 , R 4 , R 5 and R 6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 7 ring members; a group R a - R b wherein R a is a bond, O, CO, X 1 C(X 2 ), C(X 2 )X 1 , X ⁇ OI ⁇ X 1 , S, SO, SO 2 , NR C , SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 (typically 3 to 10 and more usually 5 to 10) ring members, and a C 1-8 hydrocarbyl group optionally substituted by one or more substituents selected
  • R c is hydrogen or C hydrocarbyl
  • R 3 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and R a -R b .
  • R is hydrogen, C t - 6 alkyl, fluorine or chlorine, and most preferably R 3 is hydrogen.
  • R 5 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and R a -R b .
  • R 3 and R 5 are both hydrogen.
  • R 4 is selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members (typically 3 to 10 and more usually 5 to 10 ring members), and a group R a -R b .
  • R 4 is selected from hydrogen, halogen, a heterocyclic group and a group R a -R b wherein R a is a bond, O, CO, X*C(X 2 ), C(X 2 )X 1 , X 1 C(X 2 )X 1 , S, SO, SO 2 , NR°, SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 5 to 10 ring members, and a Ci-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-Q- 4 hydrocarbylamino, monocyclic carbocyclic and heterocyclic groups having from 5 to 10 ring members and wherein one or more carbon atoms ofthe Q-s hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C ,
  • R 4 one particular group of compounds is the group in which R 4 is selected from hydrogen, halogen, a heterocyclic group, a group O-Het where Het is a heterocyclic group having from 5 to 10 ring members, Cj. 6 alkyl, C ⁇ - 6 alkoxy, C(O)NR c R b and SO 2 NR c R b wherein R b is hydrogen or C ⁇ alkyl.
  • R 6 is preferably selected from hydrogen, methyl, amino, fluorine and chlorine, and more preferably hydrogen and amino. Most preferably, R 6 is hydrogen.
  • the compounds ofthe formula (I) may be such that when R 1 is SO 2 NR 7 R 8 , neither of R 7 and R 8 is a Ci- 8 hydrocarbyl group in which the carbon atom attached to the nitrogen atom ofthe group SO 2 NR 7 R 8 is substituted by an oxo group.
  • the compounds ofthe formula (I) may be such that R 1 is other than the heterocyclic group N-morpholino when B is a bond and A is R 2 wherein R 2 is aryl.
  • the invention provides a compound ofthe formula (II):
  • E is a group R 12 or CH 2 -R 12a where R 12 is a substituted or unsubstituted, non-bridged, carbocyclic or heterocyclic group having from 3 to 12 ring members, other than a diazacycloalkyl moiety, and R 12a is an unsubstituted or substituted aryl or heteroaryl group having from 5 to 12 ring members;
  • B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
  • R 1 is hydrogen or a group selected from SO 2 R b , SO 2 NR 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
  • R 3 , R 4 , R 5 and R 6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a - R b wherein R a is a bond, O, CO, X 1 C(X 2 ), C(X 2 )X l , X 1 C(X 2 )X 1 , S, SO, SO 2 , NR C , SO 2 NR c or NR°SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a C ⁇ - 8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C M hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12
  • R c is hydrogen or C hydrocarbyl
  • R 7 is selected from hydrogen and a d-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-d. 4 hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Q- ⁇ hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X ⁇ OX 2 ), CO ⁇ X 1 or X ⁇ C ⁇ X 1 ;
  • R is selected from R and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
  • R 9 is selected from R 8 , COR 8 and SO 2 R 8 ; or NR R or NR R may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for the groups R 12 and R 12a can be one or more substituent groups R ° selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a -R b wherein R a is a bond, O, CO, X J C(X 2 ), C(X 2 )X 1 , X ⁇ CX ⁇ X 1 , S, SO, SO 2 , NR C , SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C ⁇ -8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, o
  • R c is hydrogen or C hydrocarbyl
  • R 12 when R 12 is an azacycloalkyl or diazacycloalkyl group, at least one nitrogen atom ofthe azacycloalkyl or diazacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group;
  • R 12 and R 12a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
  • E-B-R 1 may be other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
  • E-B-R 1 may be other than a saturated azabicyclic moiety or an imidazolyl moiety.
  • the compound ofthe formula (II) is other than one in which E is unsubstituted pyridyl or pyridylmethyl, B is a bond and R 1 is hydrogen.
  • R 3 to R 6 are other than a group R a -R b wherein R a is a bond and R b is a substituted C 3 -C 3 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
  • the invention also provides a group of novel compounds ofthe formula (III):
  • G is a group R 14 or CH 2 -R 14 where R 14 is a carbocyclic group having from 3 to 12 ring members;
  • B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
  • R 13 is a group selected from SO 2 NR 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
  • R 3 , R 4 , R 5 and R 6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a - R b wherein R a is a bond, O, CO, X l C(X 2 ), C(X 2 )X l , X 1 C(X 2 )X 1 , S, SO, SO 2 , NR C , SO 2 NR c or NR c SO 2 ; and R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Q.s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C M hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to
  • R c is hydrogen or C M hydrocarbyl;
  • R 7 is selected from hydrogen and a C ⁇ .s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C M hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the C s hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X 1 C(X 2 ), C(X 2 )X J or X ⁇ CX ⁇ X 1 ;
  • R is selected from R and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
  • R 9 is selected from R 8 , COR 8 and SO 2 R 8 ; or NR 7 R 8 or NR 7 R 9 may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N-[(morpholin-4-yl)phenyl-lH-indazole-3- carboxamide andN-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide; and further excluding; (i) compounds wherein G is phenyl, R 1 is NR 7 R 8 and B is a group - CH(CH 2 OH)CH 2 -;
  • R 3 to R 8 , G and B are as hereinbefore defined.
  • preferred compounds include those wherein G is a group R 14 wherein R 14 is an aryl group having six ring members and B is a bond or a methylene group.
  • R and R are selected from hydrogen and C M alkyl or R and R 8 together with the nitrogen atom form a saturated five or six membered heterocyclic ring having one or two heteroatoms.
  • R and R together with the nitrogen atom form a saturated heterocyclic ring selected from morpholino, piperidino, piperazino and pyrrolidino.
  • R 3 to R 8 , G and B are as hereinbefore defined.
  • preferred compounds include those wherein G is a group R 14 wherein R 14 is an aryl group having six ring members and B is a bond or a methylene group.
  • a further sub-group of novel compounds ofthe invention is represented by the formula (VII):
  • Preferred compounds ofthe formula (Nil) are those wherein R 7 is selected from hydrogen and C alkyl and R 9 is selected from hydrogen, C M alkyl and C 1 . 4 alkanoyl such as acetyl.
  • R 3 to R 6 and R b are as hereinbefore defined and R 11 represents hydrogen or one or more substituents selected from halogen, C alkyl, C M alkoxy, trifluoromethyl and trifluoromethoxy.
  • the group SO 2 R b is attached to the r ⁇ et ⁇ -position ofthe benzene ring. In another embodiment, the group SO 2 R is attached to the j? ⁇ r ⁇ -position ofthe benzene ring.
  • Preferred compounds are those in which R 11 is hydrogen.
  • the invention provides a compound ofthe formula (IX):
  • R 1 is hydrogen when R 15a is aryl or, when R 15a is other than aryl, R 1 is hydrogen or a group selected from SO 2 R b , SO 2 ⁇ R 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members; and the optional substituents for the groups R 15 and R 15a can be one or more substituent groups R 10 selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group R a -R b wherein R a is a bond, O, CO, X 1 C(X 2 ), C(X 2 )X !
  • R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C ⁇ s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C ⁇ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C ⁇ -8 hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X l C(X 2 ), C X ⁇ X 1 or X ⁇ C ⁇ X 1 ; provided that when R 15a is aryl it is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms
  • R 15 and R 15a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
  • the invention also provides a group of novel compounds ofthe formula (X):
  • L is a group R 16 or CH 2 -R 16 where R 16 is a substituted or unsubstituted heteroaryl group other than imidazole, the heteroaryl group having from 5 to 12 ring members, at least one of which is nitrogen;
  • R 1 is hydrogen or a group selected from SO 2 R b , SO 2 NR 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
  • R 3 , R 4 , R 5 and R 6 are as hereinbefore defined, provided that R 4 and R 5 cannot both be methoxy; and the optional substituents for R 16 can be one or more substituent groups R 10 as hereinbefore defined; but excluding compounds wherein all of R 3 to R 6 are hydrogen and L-B-R 1 defines an unsubstituted pyridyl or pyridylmethyl group.
  • the compound ofthe formulae (IX) or (X) may be other than a compound in which J is unsubstituted pyridyl or pyridylmethyl, B is a bond and R 1 is hydrogen.
  • one sub-group of compounds is represented by the formula (XI):
  • R 17 is hydrogen, B-R 1 or R 10 , and wherein R 4 , B-R 1 and R 10 are as
  • a preferred sub-group of compounds within formula (XI) can be represented by the formula (XII):
  • R 17 is hydrogen, B-R 1 or R 10 , and wherein R 4 , B-R 1 and R 10 are as hereinbefore defined.
  • Another group of novel compounds ofthe invention is the group of compounds of the formula (XN):
  • R 18 is selected from hydrogen, halogen, and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
  • R 19 is selected from hydrogen and amino, provided that at least one of R 18 and R 19 is other than hydrogen;
  • the aryl group R is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms selected from C, ⁇ , S and O, by a group selected from SO 2 R b , SO 2 ⁇ R 7 R 8 , CONR 7 R 8 , NR 7 R 9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members.
  • Preferred compounds ofthe formula (XN) are those wherein R 18 is halogen, especially iodine or chlorine, and R 19 is hydrogen.
  • Another group of novel compounds ofthe invention is the group of compounds of the formula (XNI):
  • R b is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a -s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C 1 .
  • hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the C ⁇ - 8 hydrocarbyl group may optionally be replaced by O, S, SO, SO 2 , NR C , X 1 C(X 2 ), C(X 2 )X ! or X 1 C(X 2 )X 1 ;
  • R c is hydrogen or C hydrocarbyl
  • the compounds do not contain a benzene ring substituted by a pair of meta-o ⁇ ented carboxamido moieties.
  • J-B-R 1 and L-B- R 1 are other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
  • J-B-R 1 and L-B-R 1 are other than a saturated azabicyclic moiety or an imidazolyl moiety.
  • R 3 to R 6 are each other than a group R a -R b wherein R a is a bond and R b is a substituted C 3 -C 8 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
  • the groups E, G, J and L are sub-groups ofthe group A defined in relation to compounds ofthe formula (I).
  • the groups R 12 , R 12 a and R 14 are sub-groups ofthe group R 2
  • the group R 13 is a sub-group ofthe group R 1 .
  • the general and specific preferences, embodiments and examples set out above in relation to A, R 1 and R 2 apply also to the sub-groups E, G, R 13 , R 12 , R 12a and R 14 .
  • novel compounds ofthe formulae (IX) to (XNI) defined above are sub-groups ofthe formula (I). Except where the context dictates otherwise, the general and specific definitions of substituent groups, and the general and specific definitions, preferences and examples set out for each ofthe moieties R 1 to R 10 , A and B apply also to compounds ofthe formulae (IX) to (XNI).
  • each general and specific preference, embodiment and example ofthe groups R 1 may be combined with each general and specific preference, embodiment and example ofthe groups R 2 and/or R 3 and/or R 4 and/or R 5 and/or R 6 and/or R 7 and/or R 8 and/or R 9 and/or R 10 and/or A and/or B and their associated sub-groups, and that all such combinations are embraced by this application.
  • the various functional groups and substituents making up the compounds ofthe formula (I) are typically chosen such that the molecular weight ofthe compound of the formula (I) does not exceed 1000. More usually, the molecular weight ofthe compound will be less than 750, for example less than 700, or less than 650, or less than 600, or less than 550. More preferably, the molecular weight is less than 525 and, for example, is 500 or less.
  • 5-iodo-lH-indazole-3-carboxylic acid (6-methyl-pyridazin-3-yl)-amide; 5-chloro-lH-indazole-3-carboxylic acid (5-ethyl-[l,3,4]thiadiazol-2-yl)-amide;
  • compositions comprising a novel compound as hereinbefore defined and a pharmaceutically acceptable carrier also form part ofthe invention.
  • the invention also provides a novel compound as hereinbefore defined for use in medicine, for example for one or more ofthe uses set out above in relation to compounds ofthe formula (I).
  • Acid addition salts may be formed with a wide variety of acids, both inorganic and organic.
  • acid addition salts include salts formed with hydrochloric, hydriodic, phosphoric, nitric, sulphuric, citric, lactic, succinic, maleic, malic, isethionic, fumaric, benzenesulphonic, toluenesulphonic, methanesulphonic, ethanesulphonic, naphfhalenesulphonic, valeric, acetic, propanoic, butanoic, malonic, glucuronic and lactobionic acids.
  • a salt may be formed with a suitable cation.
  • suitable inorganic cations include, but are not limited to, alkali metal ions such as Na + and K + , alkaline earth cations such as Ca 2+ and Mg 2+ , and other cations such as Al 3+ .
  • suitable organic cations include, but are not limited to, ammonium ion (i.e., NH 4 + ) and substituted ammonium ions (e.g., NH 3 R + , NH 2 R 2 + , NHR 3 + , NR ⁇ .
  • Examples of some suitable substituted ammonium ions are those derived from: ethylamine, diethylamine, dicyclohexylamine, triethylamine, butylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, benzylamine, phenylbenzylamine, choline, meglumine, and tromethamine, as well as amino acids, such as lysine and arginine.
  • An example of a common quaternary ammonium ion is N(CH 3 ) + . Where the compounds ofthe formula (I) contain an amine function, these may form quaternary ammonium salts, for example by reaction with an alkylating agent according to methods well known to the skilled person. Such quaternary ammonium compounds are within the scope of formula (I).
  • Compounds ofthe formula (I) containing an amine function may also form N- oxides.
  • a reference herein to a compound ofthe formula (I) that contains an amine function also includes the N-oxide.
  • N-oxide may be oxidised to form an N-oxide.
  • N- oxides are the N-oxides of a tertiary amine or a nitrogen atom of a nitrogen- containing heterocycle.
  • N-Oxides can be formed by treatment ofthe corresponding amine with an oxidizing agent such as hydrogen peroxide or a per-acid (e.g. a peroxycarboxylic acid), see for example Advanced Organic Chemistry, by Jerry March, 4 th Edition, Wiley Interscience, pages. More particularly, N-oxides can be made by the procedure of L. W. Deady (Syn. Comm. 1977, 7, 509-514) in which the amine compound is reacted with w-chloroperoxybenzoic acid (MCPBA), for example, in an inert solvent such as dichloromethane.
  • MCPBA w-chloroperoxybenzoic acid
  • Esters such as carboxylic acid esters and acyloxy esters ofthe compounds of formula (I) bearing a carboxylic acid group or a hydroxyl group are also embraced by Formula (I).
  • formula (I) Also encompassed by formula (I) are any polymorphic forms ofthe compounds, solvates (e.g. hydrates), complexes (e.g. inclusion complexes or clathrates with compounds such as cyclodextrins, or complexes with metals) ofthe compounds, and pro-drugs ofthe compounds.
  • prodrugs is meant for example any compound that is converted in vivo into a biologically active compound ofthe formula (I).
  • metabolically labile esters examples include those ofthe formula -
  • C ⁇ aminoalkyl e.g., aminoethyl; 2-(N,N-diethylamino)ethyl; 2-(4-morpholino)ethyl); and acyl0xy-Q. 7 a.kyl
  • acyloxymethyl e.g., acyloxymethyl; acyloxyethyl; pivaloyloxymethyl; acetoxymethyl; 1-acetoxyethyl;
  • prodrugs are activated enzymatically to yield the active compound, or a compound which, upon further chemical reaction, yields the active compound (for example, as in ADEPT, GDEPT, LIDEPT, etc.).
  • the prodrug may be a sugar derivative or other glycoside conjugate, or may be an amino acid ester derivative.
  • the compounds ofthe formula (I) are inhibitors of cyclin dependent kinases. As such, they are expected to be useful in providing a means of arresting, or recovering control of, the cell cycle in abnormally dividing cells. It is therefore anticipated that the compounds will prove useful in treating or preventing proliferative disorders such as cancers. It is also envisaged that the compounds ofthe invention will be useful in treating conditions such as viral infections, autoimmune diseases and neurodegenerative diseases for example.
  • CDKs play a role in the regulation ofthe cell cycle, apoptosis, transcription, differentiation and CNS function. Therefore, CDK inhibitors could be useful in the treatment of diseases in which there is a disorder of proliferation, apoptosis or differentiation such as cancer. In particular RB+ve tumours may be particularly sensitive to CDK inhibitors.
  • cancers which may be inhibited include, but are not limited to, a carcinoma, for example a carcinoma ofthe bladder, breast, colon (e.g. colorectal carcinomas such as colon adenocarcinoma and colon adenoma), kidney, epidermal, liver, lung, for example adenocarcinoma, small cell lung cancer and non-small cell lung carcinomas, oesophagus, gall bladder, ovary, pancreas e.g.
  • a carcinoma for example a carcinoma ofthe bladder, breast, colon (e.g. colorectal carcinomas such as colon adenocarcinoma and colon adenoma), kidney, epidermal, liver, lung, for example adenocarcinoma, small cell lung cancer and non-small cell lung carcinomas, oesophagus, gall bladder, ovary, pancreas e.g.
  • exocrine pancreatic carcinoma, stomach, cervix, thyroid, prostate, or skin for example squamous cell carcinoma
  • a hematopoietic tumour of lymphoid lineage for example leukemia, acute lymphocytic leukemia, B-cell lymphoma, T-cell lymphoma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, hairy cell lymphoma, or Burkett's lymphoma
  • a hematopoietic tumor of myeloid lineage for example acute and chronic myelogenous leukemias, myelodysplastic syndrome, or promyelocytic leukemia
  • thyroid follicular cancer a tumour of mesenchymal origin, for example fibrosarcoma or habdomyosarcoma, ; a tumor ofthe * central or peripheral nervous system, for example astrocytoma, neuroblastoma, glioma or schwannoma; mel
  • CDKs are also known to play a role in apoptosis, proliferation, differentiation and transcription and therefore CDK inhibitors could also be useful in the treatment of the following diseases other than cancer; viral infections, for example herpes virus, pox virus, Epstein-Barr virus, Sindbis virus, adenovirus, HIN, HPN, HCN and HCMN; prevention of AIDS development in HIN-infected individuals; chronic inflammatory diseases, for example systemic lupus erythematosus, autoimmune mediated glomerulonephritis, rheumatoid arthritis, psoriasis, inflammatory bowel disease, and autoimmune diabetes mellitus; cardiovascular diseases for example cardiac hypertrophy, restenosis, atherosclerosis; neurodegenerative disorders, for example Alzheimer's disease, AIDS-related dementia, Parkinson's disease, amyotropic lateral sclerosis, retinitis pigmentosa, spinal muscular atropy and cerebellar degeneration; glomerulonephritis; mye
  • cyclin-dependent kinase inhibitors can be used in combination with other anticancer agents.
  • the cytotoxic activity of cyclin-dependent kinase inhibitor flavopiridol has been used with other anticancer agents in combination therapy.
  • the disease or condition comprising abnormal cell growth in one embodiment is a cancer.
  • cancers include breast cancer, ovarian cancer, colon cancer, prostate cancer, oesophageal cancer, squamous cancer and non-small cell lung carcinomas.
  • the coupling reaction between the amine and the carboxylic acid (XNII) can be carried out by forming an activated derivative ofthe acid such as an acid chloride (e.g. by reaction with thionyl chloride), and then reacting the acid chloride with the amine, for example by the method described in Zh. Obs. Khim. 31, 201 (1961), and the method described in US 3,705,175.
  • the coupling reaction between the carboxylic acid (XNII) and the amine can be carried out in the presence of an amide coupling reagent ofthe type commonly used to form peptide linkages.
  • an amide coupling reagent ofthe type commonly used to form peptide linkages.
  • examples of such reagents include 1,3-dicyclohexylcarbodiimide (DCC) (Sheehan et al, J. Amer. Chem Soc. 1955, 77, 1067), l-ethyl-3-(3'-dimethylaminopropyl)carbodiimide (EDCI) (Sheehan et al, J. Org.
  • uronium-based coupling agents such as O-(7-azabenzotriazol-l-yl)-NNN',N'-tetramethyluronium hexafluorophosphate (HATU) (L. A. Carpino, J. Amer. Chem. Soc, 1993, 115, 4397) and phosphonium-based coupling agents such as 1-benzo- triazolyloxytris(pyrrolidino)phosphonium hexafluorophosphate (PyBOP) (Castro et al, Tetrahedron Letters, 1990, 31, 205).
  • a preferred coupling reagent is HATU.
  • Carbodiimide-based coupling agents are advantageously used in combination withl -hydroxybenzotriazole (HOBt) (Konig et al, Chem. Ber. , 103, 708, 2024- 2034).
  • Preferred coupling reagents include EDC and DCC in combination with HOBt.
  • the coupling reaction is typically carried out in a non-aqueous, non-protic solvent such as dichloromethane, dimethylformamide or ⁇ -methylpyrrolidine.
  • a non-aqueous, non-protic solvent such as dichloromethane, dimethylformamide or ⁇ -methylpyrrolidine.
  • the reaction can be carried out at room temperature or, where the reactants are less reactive (for example in the case of electron-poor anilines bearing electron withdrawing groups such as sulphonamide groups) at an appropriately elevated temperature.
  • the reaction may be carried out in the presence of a non-interfering base, for example a tertiary amine such as triethylamine or NN-diisopropylethylamine.
  • Carboxylic acids ofthe formula (XNII) can be obtained commercially.
  • compounds ofthe formula (XNII) can be prepared from compounds ofthe formula (XNIII): (XNIII) by a sequence of reactions involving ring-opening, diazotisation, reduction and cyclisation.
  • Ring opening ofthe substituted isatin compound to give an ortho- aminophenyl-glyoxylic acid derivative can be achieved using an aqueous alkali such as sodium hydroxide with moderate heating, for example to a temperature of 35°C.
  • the amine can then be converted to the diazonium salt by treatment with nitrous acid (for example generated from sodium nitrite and sulphuric acid) at a reduced temperature (e.g. approximately 5°C).
  • the diazonium salt is reduced to form a hydrazine using a reducing agent such as tin (II) chloride and is then cyclised to the indazole by a cyclo-condensation reaction.
  • ⁇ -protected anilines can be subjected to ortho- lithiation and the lithiated intermediate reacted with diethyl oxalate to give an ⁇ - ketoester which cyclises to give an isatin upon deprotection ofthe amino group.
  • substituted isatins can be formed by the ⁇ -dibromination of 2-oxo-indolines and subsequent hydrolysis ofthe resulting dibromo-compounds.
  • An alternative route to compounds ofthe formula (I) involves the reaction of a substituted phenyl acetic acid amide compound ofthe formula (XIX):
  • nitrous acid or an alkyl nitrite at a reduced temperature (e.g. lower than 20°C and preferably below 0°C) in the presence of a mineral acid such as hydrochloric acid or sulphuric acid or a mixture of hydrochloric acid and acetic acid, for example as described in US 3,705,175.
  • a mineral acid such as hydrochloric acid or sulphuric acid or a mixture of hydrochloric acid and acetic acid, for example as described in US 3,705,175.
  • Compounds of the formula (I) can also be prepared from other compounds ofthe formula (I) bearing suitable substituents and suitable reactive groups.
  • compounds wherein one or more of R 3 to R 6 are bromine or iodine, particularly iodine, can be used as intermediates for the preparation of other compounds ofthe formula (I).
  • the aldehyde or ketone group is readily regenerated by hydrolysis using a large excess of water in the presence of acid.
  • An amine group may be protected, for example, as an amide (-NRCO-R) or a urethane (-NRCO-OR), for example, as: a methyl amide (-NHCO-CH 3 ); a benzyloxy amide (-NHCO-OCH2 H5, -NH-Cbz); as a t-butoxy amide (-NHCO-OC(CH 3 ) 3 , -NH-Boc); a 2-biphenyl-2-propoxy amide (-NHCO-OC(CH 3 ) 2 C 6 H 4 C 6 H 5 , -NH- Bpoc), as a 9-fluorenylmethoxy amide (-NH-Fmoc), as a 6-nitroveratryloxy amide (-NH-Nvoc), as a 2-trimethylsilylethyloxy amide (-NH-Teoc), as a 2,2,2- trichloroethyloxy amide (-NH-Troc), as an allyl
  • a carboxylic acid group may be protected as an ester for example, as: an C 1 -7 alkyl ester (e.g., a methyl ester; a t-butyl ester); a C 1 .
  • haloalkyl ester e.g., a C ⁇ trihaloalkyl ester); a triC ⁇ alkylsilyl-C ⁇ alkyl ester; or a C 5 . 2 o aryl-C ⁇ - 7 alkyl ester (e.g., a benzyl ester; a nitrobenzyl ester); or as an amide, for example, as a methyl amide.
  • the invention also provides compounds ofthe formula (I) as hereinbefore defined in the form of pharmaceutical compositions.
  • compositions can be in any form suitable for oral, parenteral, topical, intranasal, ophthalmic, otic, rectal, intra-vaginal, or transdermal administration.
  • compositions are intended for parenteral administration, they can be formulated for intravenous, intramuscular, intraperitoneal, subcutaneous administration or for direct delivery into a target organ or tissue by injection, infusion or other means of delivery.
  • compositions contaimng compounds ofthe formula (I) can be formulated in accordance with known techniques, see for example, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, PA, USA.
  • tablet compositions can contain a unit dosage of active compound together with an inert diluent or carrier such as a sugar or sugar alcohol, eg; lactose, sucrose, sorbitol or mannitol; and/or a non-sugar derived diluent such as sodium carbonate, calcium phosphate, calcium carbonate, or a cellulose or derivative thereof such as methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, and starches such as corn starch. Tablets may also contain such standard ingredients as binding and granulating agents such as polyvinylpyrrolidone, disintegrants (e.g.
  • swellable crosslinked polymers such as crosslinked carboxymethylcellulose
  • lubricating agents e.g. stearates
  • preservatives e.g. parabens
  • antioxidants e.g. BHT
  • buffering agents for example phosphate or citrate buffers
  • effervescent agents such as citrate/bicarbonate mixtures.
  • Capsule formulations may be ofthe hard gelatin or soft gelatin variety and can contain the active component in solid, semi-solid, or liquid form.
  • Gelatin capsules can be formed from animal gelatin or synthetic or plant derived equivalents thereof.
  • the solid dosage forms can be coated or un-coated, but typically have a coating, for example a protective film coating (e.g. a wax or varnish) or a release controlling coating.
  • a protective film coating e.g. a wax or varnish
  • the coating e.g. a Eudragit TM type polymer
  • the coating can be designed to release the active component at a desired location within the gastro-intestinal tract.
  • the coating can be selected so as to degrade under certain pH conditions within the gastrointestinal tract, thereby selectively release the compound in the stomach or in the ileum or duodenum.
  • the drug can be presented in a solid matrix comprising a release controlling agent, for example a release delaying agent which may be adapted to selectively release the compound under conditions of varying acidity or alkalinity in the gastrointestinal tract.
  • a release controlling agent for example a release delaying agent which may be adapted to selectively release the compound under conditions of varying acidity or alkalinity in the gastrointestinal tract.
  • the matrix material or release retarding coating can take the form of an erodible polymer (e.g. a maleic anhydride polymer) which is substantially continuously eroded as the dosage form passes through the gastrointestinal tract.
  • compositions for topical use include ointments, creams, sprays, patches, gels, liquid drops and inserts (for example intraocular inserts). Such compositions can be formulated in accordance with known methods.
  • compositions for parenteral administration are typically presented as sterile aqueous or oily solutions or fine suspensions, or may be provided in finely divided sterile powder form for making up extemporaneously with sterile water for injection.
  • formulations for rectal or intra- vaginal administration include pessaries and suppositories which may be, for example, formed from a shaped moldable or waxy material containing the active compound.
  • compositions for administration by inhalation may take the form of inhalable powder compositions or liquid or powder sprays, and can be administrated in standard form using powder inhaler devices or aerosol dispensing devices. Such devices are well known.
  • the powdered formulations typically comprise the active compound together with an inert solid powdered diluent such as lactose.
  • a formulation intended for oral administration may contain from 0.1 milligrams to 2 grams of active ingredient, more usually from 10 milligrams to 1 gram, for example, 50 milligrams to 500 milligrams.
  • the active compound will be administered to a patient in need thereof (for example a human or animal patient) in an amount sufficient to achieve the desired therapeutic effect.
  • the compounds ofthe formula (I) will useful in the prophylaxis or treatment of a range of disease states or conditions mediated by cyclin dependent kinases. Examples of such disease states and conditions are set out above.
  • Compounds ofthe formula (I) are generally administered to a subject in need of such administration, for example a human or animal patient, preferably a human.
  • the compounds will typically be administered in amounts that are therapeutically or prophylactically useful and which generally are non-toxic.
  • the benefits of administering a compound ofthe formula (I) may outweigh the disadvantages of any toxic effects or side effects, in which case it may be considered desirable to administer compounds in amounts that are associated with a degree of toxicity.
  • a typical daily dose ofthe compound can be in the range from 100 picograms to 100 milligrams per kilogram of body weight, more typically 10 nanograms to 10 milligrams per kilogram of bodyweight although higher or lower doses may be administered where required.
  • the quantity of compound administered will be commensurate with the nature ofthe disease or physiological condition being treated and will be at the discretion ofthe physician.
  • the compounds ofthe formula (I) can be administered as the sole therapeutic agent or they can be administered in combination therapy with one of more other compounds for treatment of a particular disease state, for example a neoplastic disease such as a cancer as hereinbefore defined.
  • a neoplastic disease such as a cancer as hereinbefore defined.
  • other therapeutic agents that may be administered together (whether concurrently or at different time intervals) with the compounds ofthe formula (I) include cytotoxic agents, agents that prevent cell proliferation or radiotherapy.
  • agents include but are not limited to topoisomerase inhibitors, alkylating agents, antimetabolites, DNA binders and microtubule inhibitors, such as cisplatin, cyclophosphamide, doxorubicin, irinotecan, fludarabine, 5FU, taxanes and mitomycin C.
  • the invention provides the use ofthe compounds ofthe formula (I) as hereinbefore defined as antifungal agents.
  • the compounds ofthe formula (I) may be used in animal medicine (for example in the treatment of mammals such as humans), or in the treatment of plants (e.g. in agriculture and horticulture), or as general antifungal agents, for example as preservatives and disinfectants.
  • the invention provides a compound ofthe formula (I) as hereinbefore defined for use in the prophylaxis or treatment of a fungal infection in a mammal such as a human.
  • compounds ofthe invention may be administered to human patients suffering from, or at risk of infection by, topical fungal infections caused by among other organisms, species of Candida, Trichophyton, Microsporum or Epidermophyton, or in mucosal infections caused by Candida albicans (e.g. thrush and vaginal candidiasis).
  • the compounds ofthe invention can also be administered for the treatment or prophylaxis of systemic fungal infections caused by, for example, Candida albicans, Cryptococcus neoformans, Aspergillus flavus, Aspergillus fumigatus, Coccidiodies, Paracoccidioides, Histoplasma or Blastomyces.
  • the invention provides an antifungal composition for agricultural (including horticultural) use, comprising a compound ofthe formula (I) together with an agriculturally acceptable diluent or carrier.
  • the invention further provides a method of treating an animal (including a mammal such as a human), plant or seed having a fungal infection, which comprises treating said animal, plant or seed, or the locus of said plant or seed, with an effective amount of a compound ofthe formula (I).
  • the invention also provides a method of treating a fungal infection in a plant or seed which comprises treating the plant or seed with an antifungally effective amount of a fungicidal composition as hereinbefore defined.
  • Differential screening assays may be used to select for those compounds ofthe present invention with specificity for non-human CDK enzymes.
  • Compounds which act specifically on the CDK enzymes of eukaryotic pathogens can be used as antifungal or anti-parasitic agents.
  • Inhibitors ofthe Candida CDK kinase, CKSI can be used in the treatment of candidiasis.
  • the differential screening assays can be used to identify anti-fungal agents which may have therapeutic value in the treatment of aspergillosis by making use ofthe CDK genes cloned from yeast such as Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, or Aspergillus terreus, or where the mycotic infection is mucon-nycosis, the CDK assay can be derived from yeast such as Rhizopus arrhizus, Rhizopus oryzae, Absidia corymbifera, Absidia ramosa, or Mucorpusillus. Sources of other CDK enzymes include the pathogen Pneumocystis carinii.
  • the compounds ofthe formula (I) can be administered alone or in admixture with a pharmaceutical carrier selected in accordance with the intended route of administration and standard pharmaceutical practice.
  • a pharmaceutical carrier selected in accordance with the intended route of administration and standard pharmaceutical practice.
  • they may be administered orally, parenterally, intravenously, intramuscularly or subcutaneously by means ofthe formulations described above in the section headed "Pharmaceutical Formulations".
  • the daily dosage level of the antifungal compounds ofthe formula (I) be from 0.01 to 10 mg/kg (in divided doses), depending on inter alia the potency ofthe compounds when administered by either the oral or parenteral route.
  • Tablets or capsules ofthe compounds may contain, for example, from 5 mg. to 0.5 g of active compound for administration singly or two or more at a time as appropriate. The physician in any event will determine the actual dosage (effective amount) which will be most suitable for an individual patient and it will vary with the age, weight and response ofthe particular patient.
  • the antifungal compounds of formula (I) can be administered in the form of a suppository or pessary, or they may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder.
  • they can be incorporated into a cream consisting of an aqueous emulsion of polyethylene glycols or liquid paraffin; or they can be incorporated, at a concentration between 1 and 10%, into an ointment consisting of a white wax or white soft paraffin base together with such stabilizers and preservatives as may be required.
  • anti-fungal agents developed with such differential screening assays can be used, for example, as preservatives in foodstuff, feed supplement for promoting weight gain in livestock, or in disinfectant formulations for treatment of non-living matter, e.g., for decontaminating hospital equipment and rooms.
  • side by side comparison of inhibition of a mammalian CDK and an insect CDK such as the Drosophilia CDK5 gene (Hellmich et al. (1994) FEBS Lett 356:317-21)
  • the present invention expressly contemplates the use and formulations ofthe compounds ofthe invention in insecticides, such as for use in management of insects like the fruit fly.
  • certain ofthe subject CDK inhibitors can be selected on the basis of inhibitory specificity for plant CDK's relative to the mammalian enzyme.
  • a plant CDK can be disposed in a differential screen with one or more ofthe human enzymes to select those compounds of greatest selectivity for inhibiting the plant enzyme.
  • the present invention specifically contemplates formulations ofthe subject CDK inhibitors for agricultural applications, such as in the form of a defoliant or the like.
  • the compounds ofthe invention may be used in the form of a composition formulated as appropriate to the particular use and intended purpose.
  • the compounds may be applied in the form of dusting powders, or granules, seed dressings, aqueous solutions, dispersions or emulsions, dips, sprays, aerosols or smokes.
  • Compositions may also be supplied in the form of dispersible powders, granules or grains, or concentrates for dilution prior to use.
  • Such compositions may contain such conventional carriers, diluents or adjuvants as are known and acceptable in agriculture and horticulture and they are manufactured in accordance with conventional procedures.
  • compositions may also incorporate other active ingredients, for example, compounds having herbicidal or insecticidal activity or a further fungicide.
  • the compounds and compositions can be applied in a number of ways, for example they can be applied directly to the plant foliage, stems, branches, seeds or roots or to the soil or other growing medium, and they may be used not only to eradicate disease, but also prophylactically to protect the plants or seeds from attack.
  • the compositions may contain from 0.01 to 1 wt.% ofthe active ingredient. For field use, likely application rates ofthe active ingredient may be from 50 to 5000 g/hectare.
  • the invention also contemplates the use ofthe compounds ofthe formula (I) in the control of wood decaying fungi and in the treatment of soil where plants grow, paddy fields for seedlings, or water for perfusion. Also contemplated by the invention is the use ofthe compounds ofthe formula (I) to protect stored grain and other non-plant loci from fungal infestation.
  • Mass Spec Detector Micromass Platform LC PDA Detector: Waters 996 PDA
  • Mass Spec Detector Waters-Micromass ZQ PDA Detector: Waters 2996 PDA
  • indazole-3 -carboxylic acid (Fluka) (405 mg, 2.5 mmol, 1.0 equiv) in dichloromethane (10 ml) was added an amine or appropriately substituted aniline (3.0 mmol, 1.2 equiv), NN-diisopropylethylamine (1.6 ml, 9.0 mmol, 3.6 equiv) and O-(7-azabenzotriazol-l-yl)-NNN' ) N'-tetramethyluronium hexafluorophosphate (1.05 g, 2.75 mmol, 1.1 equiv).
  • Procedure B was followed using 5-Nitro-lH-indazole-3-carboxylic acid (Example 17A) and (4-amino-phenyl)-N-methyl-methane sulphonamide. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was further purified by preparative HPLC: LCMS 3.30 min, m/z [M+H] + 390.
  • Procedure C was followed using bis(tri-t-butylphosphine)palladium (0) (Strem) and 3,5-dimethylisoxazole-4-boronic acid (Maybridge). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 5 mg (11%); LCMS 3.54 min, m/z [M+H] + 440.
  • 2-Thiazolylzinc bromide (2 ml of a 0.5M solution in THF, 1.0 mmol, 10 equiv) was added and the mixture was heated to 195 °C for 15 minutes using 100 watts in a CEM microwave. The reaction was quenched with methanol and evaporated to dryness. The title compound was purified by Biotage (SiO 2 ), eluted with 80% EtO Ac-petrol, to afford 18 mg (42%); LCMS 3.27 min, m/z [M+H] + 428.
  • Example 61 A The product of Example 61 A was reacted with 5-iodo indazole-3 -carboxylic acid using method B to give the title compound.
  • Example 63A To the nitro-lH-indazole-3-carboxylic acid (1 equiv.) of Example 63A in DMF (0.3 M) was added EDC (1.2 equiv.), HOBT (1.2 equiv.), NMM (1.2 equiv.) and then 4- methylsulphamoylmethyl-phenylamine (1.3 equiv.) at room temperature. The reaction was heated to 70 °C for 2 hours and then stirred at room temperature for 48 hours. Water was added to the reaction mixture and the precipitated product was filtered. The solid was washed with water, then a small volume of MeOH, and then dried in a vacuum oven to leave a yellow solid.
  • EDC 1.2 equiv.
  • HOBT 1.2 equiv.
  • NMM 1.2 equiv.
  • 4- methylsulphamoylmethyl-phenylamine 1.3 equiv.
  • N-(4-Amino-benzyl)-acetamide produced by the method of Example 15A was reacted with 5-chloro- lH-indazole-3-carboxylic acid following procedure B to give the title compound.
  • ⁇ 33 P-ATP which remains unincorporated into the histone HI is separated from phosphorylated histone HI on a Millipore MAPH filter plate.
  • the wells ofthe MAPH plate are wetted with 0.5% orthophosphoric acid, and then the results ofthe reaction are filtered with a Millipore vacuum filtration unit through the wells. Following filtration, the residue is washed twice with 200 ⁇ l of 0.5% orthophosphoric acid. Once the filters have dried, 25 ⁇ l of Microscint 20 scintillant is added, and then counted on a Packard Topcount for 30 seconds.
  • the % inhibition ofthe CDK2 activity is calculated and plotted in order to determine the concentration of test compound required to inhibit 50% ofthe CDK2 activity (ICso).
  • the compounds of Examples 3 to 19, 21 to 76, 78, 80, 81 and 84 to 87 each have IC 50 values of less than lOO ⁇ M or provide at least 50% inhibition ofthe CDK2 activity at a concentration of 50 ⁇ M.
  • a tablet composition containing a compound ofthe formula (I) is prepared by mixing 50mg ofthe compound with 197mg of lactose (BP) as diluent, and 3mg magnesium stearate as a lubricant and compressing to form a tablet in known manner.
  • BP lactose
  • a capsule formulation is prepared by mixing lOOmg of a compound ofthe formula (I) with lOOmg lactose and filling the resulting mixture into standard opaque hard gelatin capsules.
  • the antifungal activity ofthe compounds ofthe formula (I) is determined using the following protocol .
  • the compounds are tested against a panel of fungi including Candida parpsilosis, Candida tropicalis, Candida albicans-ATCC 36082 and Cryptococcus neoformans.
  • the test organisms are maintained on Sabourahd Dextrose Agar slants at 4 °C.
  • Singlet suspensions of each organism are prepared by growing the yeast overnight at 27 °C on a rotating drum in yeast-nitrogen base broth (YNB) with amino acids (Difco, Detroit, Mich.), pH 7.0 with 0.05 morpholine propanesulphonic acid (MOPS). The suspension is then centrifuged and washed twice with 0.85% NaCl before sonicating the washed cell suspension for 4 seconds (Branson Sonifier, model 350, Danbury, Conn.). The singlet blastospores are counted in a haemocytometer and adjusted to the desired concentration in 0.85% NaCl.
  • test compounds The activity ofthe test compounds is determined using a modification of a broth microdilution technique.
  • Test compounds are diluted in DMSO to a 1.0 mg/ml ratio then diluted to 64 ⁇ g/ml in YNB broth, pH 7.0 with MOPS (Fluconazole is used as the control) to provide a working solution of each compound.
  • MOPS Fluonazole is used as the control
  • wells 1 and 3 through 12 are prepared with YNB broth, ten fold dilutions ofthe compound solution are made in wells 2 to 11 (concentration ranges are 64 to 0.125 ⁇ g/ml).
  • Well 1 serves as a sterility control and blank for the spectrophotometric assays.
  • Well 12 serves as a growth control.
  • microtitre plates are inoculated with 10 ⁇ l in each of well 2 to 11 (final inoculum size is 10 4 organisms/ml). Inoculated plates are incubated for 48 hours at 35 °C.
  • the MIC values are determined spectrophotometrically by measuring the absorbance at 420 nm (Automatic Microplate Reader, DuPont Instruments, Wilmington, Del.) after agitation ofthe plates for 2 minutes with a vortex-mixer (Norte-Genie 2 Mixer, Scientific
  • MCC Minimal Cytolytic Concentrations
  • compositions are then used to test the activity ofthe compounds ofthe invention against tomato blight (Phytophthora infestans) using the following protocol.
  • Tomatoes (cultivar Rutgers) are grown from seed in a soil-less peat-based potting mixture until the seedlings are 10-20 cm tall. The plants are then sprayed to run-off with the test compound at a rate of 100 ppm. After 24 hours the test plants are inoculated by spraying with an aqueous sporangia suspension of Phytophthora infestans, and kept in a dew chamber overnight. The plants are then transferred to the greenhouse until disease develops on the untreated control plants.

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Abstract

The invention provides a compound of the formula (I) for use in the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase: wherein A is a group R2 or CH2-R2 where R2 is a carbocyclic or heterocyclic group having from 3 to 12 ring members; B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O; R1 is hydrogen or a group selected from SO2Rb,SO2NR7R8,CONR7R8,NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members; R3, to R9 are defined in the description but excluding the compounds N-[(morpholin-4-yl)phenyl-1H-indazole-3-carboxamide and N-[4-(acetylaminosulphonyl)phenyl-1H-indazole-3-carboxamide.

Description

1H-INDAZ0 E-3-CARB0XAMIDE COMPOUNDS AS CYC IN DEPENDENT KINASES (CDK) INHIBITORS
This invention relates to 3 -substituted indazole compounds that inhibit or modulate the activity of cyclin dependent kinases (CDK), to the use ofthe compounds in the treatment or prophylaxis of disease states or conditions mediated by cyclin dependent kinases, and to novel compounds having cyclin dependent kinase inhibitory or modulating activity. Also provided are pharmaceutical compositions containing the compounds and novel chemical intermediates.
Background of the Invention
Protein kinases constitute a large family of structurally related enzymes that are responsible for the control of a wide variety of signal transduction processes within the cell (Hardie, G. and Hanks, S. (1995) The Protein Kinase Facts Book. I and II, Academic Press, San Diego, CA). The kinases may be categorized into families by the substrates they phosphorylate (e.g., protein-tyrosine, protein-serine/threonine, lipids, etc.). Sequence motifs have been identified that generally correspond to each of these kinase families (e.g., Hanks, S.K., Hunter, T., FASEB J., 9:576-596 (1995); Knighton, et al, Science, 253:407-414 (1991); Hiles, et al, Cell, 70:419-429 (1992); Kunz, et al, Cell, 73:585-596 (1993); Garcia-Bustos, et al, EMBO J., 13:2352-2361 (1994)).
Protein kinases may be characterized by their regulation mechanisms. These mechanisms include, for example, autophosphorylation, transphosphorylation by other kinases, protein-protein interactions, protein-lipid interactions, and protein- polynucleotide interactions. An individual protein kinase may be regulated by more than one mechanism.
Kinases regulate many different cell processes including, but not limited to, proliferation, differentiation, apoptosis, motility, transcription, translation and other signalling processes, by adding phosphate groups to target proteins. These phosphorylation events act as molecular on off switches that can modulate or regulate the target protein biological function. Phosphorylation of target proteins occurs in response to a variety of extracellular signals (hormones, neurotransmitters, growth and differentiation factors, etc.), cell cycle events, environmental or nutritional stresses, etc. The appropriate protein kinase functions in signalling pathways to activate or inactivate (either directly or indirectly), for example, a metabolic enzyme, regulatory protein, receptor, cytoskeletal protein, ion channel or pump, or transcription factor. Uncontrolled signalling due to defective control of protein phosphorylation has been implicated in a number of diseases, including, for example, inflammation, cancer, allergy/asthma, disease and conditions ofthe immune system, disease and conditions ofthe central nervous system, and angiogenesis.
The process of eukaryotic cell division may be broadly divided into a series of sequential phases termed Gl, S, G2 and M. Correct progression through the various phases ofthe cell cycle has been shown to be critically dependent upon the spatial and temporal regulation of a family of proteins known as cyclin dependent kinases (CDKs) and a diverse set of their cognate protein partners termed cyclins. CDKs are cdc2 (also known as CDK1) homologous serine-threonine kinase proteins that are able to utilise ATP as a substrate in the phosphorylation of diverse polypeptides in a sequence dependent context. Cyclins are a family of proteins characterised by a homology region, containing approximately 100 amino acids, termed the "cyclin box" which is used in binding to, and defining selectivity for, specific CDK partner proteins.
Modulation ofthe expression levels, degradation rates, and activation levels of various CDKs and cyclins throughout the cell cycle leads to the cyclical formation of a series of CDK/cyclin complexes, in which the CDKs are enzymatically active. The formation of these complexes controls passage through discrete cell cycle checkpoints and thereby enables the process of cell division to continue. Failure to satisfy the pre-requisite biochemical criteria at a given cell cycle checkpoint, i.e. failure to form a required CDK/cyclin complex, can lead to cell cycle arrest and/or cellular apoptosis. Aberrant cellular proliferation, as manifested in cancer, can often be attributed to loss of correct cell cycle control. Inhibition of CDK enzymatic activity therefore provides a means by which abnormally dividing cells can have their division arrested and/or be killed. The diversity of CDKs, and CDK complexes, and their critical roles in mediating the cell cycle, provides a broad spectrum of potential therapeutic targets selected on the basis of a defined biochemical rationale.
Progression from the Gl phase to the S phase ofthe cell cycle is primarily regulated by CDK2, CDK3, CDK4 and CDK6 via association with members ofthe D and E type cyclins. The D-type cyclins appear instrumental in enabling passage beyond the Gl restriction point, where as the CDK2/cyclin E complex is key to the transition from the Gl to S phase. Subsequent progression through S phase and entry into G2 is thought to require the CDK2/cyclin A complex. Both mitosis, and the G2 to M phase transition which triggers it, are regulated by complexes of CDK 1 and the A and B type cyclins.
During Gl phase Retinoblastoma protein (Rb), and related pocket proteins such as pi 30, are substrates for CDK(2, 4, & 6)/cyclin complexes. Progression through Gl is in part facilitated by hyperphosphorylation, and thus inactivation, of Rb and pi 30 by the CDK(4/6)/cyclin-D complexes. Hyperphosphorylation of Rb and pl30 causes the release of transcription factors, such as E2F, and thus the expression of genes necessary for progression through Gl and for entry into S-phase, such as the gene for cyclin E. Expression of cyclin E facilitates formation ofthe CDK2/cyclin E complex which amplifies, or maintains, E2F levels via further phosphorylation of Rb. The CDK2/cyclin E complex also phosphorylates other proteins necessary for DNA replication, such as NPAT, which has been implicated in histone biosynthesis. Gl progression and the Gl/S transition are also regulated via the mitogen stimulated Myc pathway, which feeds into the CDK2/cyclin E pathway. CDK2 is also connected to the p53 mediated DNA damage response pathway via p53 regulation of p21 levels. p21 is a protein inhibitor of CDK2/cyclin E and is thus capable of blocking, or delaying, the Gl/S transition. The CDK2/cyclin E complex may thus represent a point at which biochemical stimuli from the Rb, Myc and p53 pathways are to some degree integrated. CDK2 and/or the CDK2/cyclin E complex therefore represent good targets for therapeutics designed at arresting, or recovering control of, the cell cycle in aberrantly dividing cells.
The exact role of CDK3 in the cell cycle is not clear. As yet no cognate cyclin partner has been identified, but a dominant negative form of CDK3 delayed cells in Gl, thereby suggesting that CDK3 has a role in regulating the Gl/S transition.
Although most CDKs have been implicated in regulation ofthe cell cycle there is evidence that certain members ofthe CDK family are involved in other biochemical processes. This is exemplified by CDK5 which is necessary for correct neuronal development and which has also been implicated in the phosphorylation of several neuronal proteins such as Tau, NUDE-1, synapsinl, DARPP32 and the
Muncl8/SyntaxinlA complex. Neuronal CDK5 is conventionally activated by binding to the p35/p39 proteins. CDK5 activity can, however, be deregulated by the binding of p25, a truncated version of p35. Conversion of p35 to p25, and subsequent deregulation of CDK5 activity, can be induced by ischemia, excitotoxicity, and β-amyloid peptide. Consequently p25 has been implicated in the pathogenesis of neurodegenerative diseases, such as Alzheimer's, and is therefore of interest as a target for therapeutics directed against these diseases.
CDK7 is a nuclear protein that has cdc2 CAK activity and binds to cyclin H. CDK7 has been identified as component ofthe TFIIH transcriptional complex which has RNA polymerase II C-terminal domain (CTD) activity. This has been associated with the regulation of HIN-1 transcription via a Tat-mediated biochemical pathway. CDK8 binds cyclin C and has been implicated in the phosphorylation ofthe CTD of RΝA polymerase II. Similarly the CDK9/cyclin-Tl complex (P-TEFb complex) has been implicated in elongation control of RΝA polymerase II. PTEF-b is also required for activation of transcription ofthe HIN-1 genome by the viral transactivator Tat through its interaction with cyclin Tl . CDK7, CDK8, CDK9 and the P-TEFb complex are therefore potential targets for anti-viral therapeutics. At a molecular level mediation of CDK/cyclin complex activity requires a series of stimulatory and inhibitory phosphorylation, or dephosphorylation, events. CDK phosphorylation is performed by a group of CDK activating kinases (CAKs) and/or kinases such as weel, Mytl and Mikl. Dephosphorylation is performed by phosphatases such as cdc25(a & c), pp2a, or KAP.
CDK/cyclin complex activity may be further regulated by two families of endogenous cellular proteinaceous inhibitors: the Kip/Cip family, or the INK family. The INK proteins specifically bind CDK4 and CDK6. ρl6Ink4 (also known as MTS1) is a potential tumour suppressor gene that is mutated, or deleted, in a large number of primary cancers. The Kip/Cip family contains proteins such as p21cipi,wafi. p27κipl and p57κi 2. As discussed previously p21 is induced by p53 and is able to inactivate the CDK2/cyclin(E/A) and CDK4/cyclin(Dl/D2/D3) complexes. Atypically low levels of p27 expression have been observed in breast, colon and prostate cancers. Conversely over expression of cyclin E in solid tumours has been shown to correlate with poor patient prognosis. Over expression of cyclin Dl has been associated with oesophageal, breast, squamous, and non- small cell lung carcinomas.
The pivotal roles of CDKs, and their associated proteins, in co-ordinating and driving the cell cycle in proliferating cells have been outlined above. Some ofthe biochemical pathways in which CDKs play a key role have also been described.
The development of monotherapies for the treatment of proliferative disorders, such as cancers, using therapeutics targeted generically at CDKs, or at specific CDKs, is therefore potentially highly desirable. CDK inhibitors could conceivably also be used to treat other conditions such as viral infections, autoimmune diseases and neuro-degenerative diseases, amongst others. CDK targeted therapeutics may also provide clinical benefits in the treatment ofthe previously described diseases when used in combination therapy with either existing, or new, therapeutic agents. CDK targeted anticancer therapies could potentially have advantages over many current antitumour agents as they would not directly interact with DNA and should therefore reduce the risk of secondary tumour development. WO 02/34721 from Du Pont discloses a class of indeno [l,2-c]pyrazol-4-ones as inhibitors of cyclin dependent kinases.
WO 01/81348 from Bristol Myers Squibb describes the use of 5-thio-, sulphinyl- and sulphonylpyrazolo[3,4-b]-pyridines as cyclin dependent kinase inhibitors.
WO 00/62778 also from Bristol Myers Squibb discloses a class of protein tyrosine kinase inhibitors.
WO 01/72745 Al from Cyclacel describes 2-substituted 4-heteroaryl-pyrimidines and their preparation, pharmaceutical compositions containing them and their use as inhibitors of cyclin-dependant kinases (CDKs) and hence their use in the treatment of proliferative disorders such as cancer, leukaemia, psoriasis and the like.
WO 99/21845 from Agouron describes 4-aminothiazole derivatives for inhibiting cyclin-dependent kinases (CDKs), such as CDK1, CDK2, CDK4, and CDK6. The invention is also directed to the therapeutic or prophylactic use of pharmaceutical compositions containing such compounds and to methods of treating malignancies and other disorders by administering effective amounts of such compounds.
WO 01/53274 from Agouron discloses as CDK kinase inhibitors a class of compounds which can comprise an amide-substituted benzene ring linked to an N- containing heterocyclic group. Although indazole compounds are not mentioned generically, one ofthe exemplified compounds comprises an indazole 3 -carboxylic acid anilide moiety linked via a methylsulphanyl group to a pyrazolopyrimidme.
WO 01/98290 (Pharmacia & Upjohn) discloses a class of 3-aminocarbonyl-2- carboxamido thiophene derivatives as protein kinase inhibitors. The compounds are stated to have multiple protein kinase activity.
GB 1301882, US 3,705,175, DE 2,135,398 (all to Egyt), and Ferenc et al, Magyar Kemikusok Lapja, 1975, 30(4), 208-215, each disclose 6,7-dimethoxyindazole-3- carboxylic acid amides as anti-inflammatory and analgesic agents. US 3,457,269 and US 3,145,215 (both to Sterling Drug) disclose indazole-3- carboxylic acid amides, including anilides, cycloaliphatic amides and pyridylamides, as hypotensive agents.
WO 01/53268 and WO 01/02369 from Agouron disclose compounds that mediate or inhibit cell proliferation through the inhibition of protein kinases such as cyclin dependent kinase or tyrosine kinase. The Agouron compounds have an aryl or heteroaryl ring attached directly or though a CH=CH or CH=N group to the 3- position of an indazole ring.
WO 02/10137 (Signal Pharmaceuticals) discloses a class of indazole derivatives as selective inhibitors of JNK kinase. The indazole derivatives have an aryl, heteroaryl or heterocyclic group linked to the indazole 3-position through an akylene or alkenylene group.
US 6,340,685 (Scios) discloses a class of bicyclic heterocyclic compounds as selective P38 MAP kinase inhibitors. Indazoles are not specifically disclosed.
WO 02/24635 (Fujisawa) discloses a class of amino alcohol derivatives as β-3 adrenergic receptor agonists. The compounds can contain an indazole 3- carboxylic acid anilide group linked to the amino alcohol group.
JP 04089489 (Nisshin), JP 03223280 (Dainippon), JP 05230057 (Dainippon), JP 04005289 (Hokuriku), JP 06135960 (Dainippon), EP 0499995 (Nisshin), EP 0623621 (Nisshin), WO 96/38420 (Nisshin), EP 0708105 (Nisshin), EP 0358903 (Dainippon), Harada et al. Chem. Pharm. Bull, 43 (11), 1912-1930 (1995), Harada et al. Chem. Pharm. Bull, 44 (12), 2205-2212 (1996) and Morie et al. Synthetic Communications, 27(4), 559-566 (1997) each disclose indazole 3-carboxamides in which the amide nitrogen is linked to a non-aromatic cyclic amino group. The compounds are described as being active as 5-HT receptor modulators.
EP 0410509 (Duphar) discloses, as 5-HT receptor antagonists, a class of indazole 3- carboxamides in which the amide nitrogen is linked to an imidazolylmethyl group. Indazole carboxylic acid derivatives are also disclosed as 5-HT receptor modulators in WO 93/03725 (SmithKline Beecham), EP 0261964 (Beecham), EP 0517984 (Merrell Dow), US 5,654,320 (Eli Lilly), EP 0908452 (Eli Lilly), EP 0908459 (Eli Lilly) and EP 0732333 (Eli Lilly).
US 5,190,953 (A.H. Robins) describes a class of azabicyclic compounds that can contain an indazole group and which are stated to increase gastric motility.
US 5,273,972 (A.H.Robins), US 5,318,977 (Searle), WO 00/63215 (Sanofi- Synthelabo), WO 02/32416 (Depomed), WO 95/27490 (Sandoz), DE 3827253 (Sandoz), WO 91/09593 (Beecham), WO 92/05174 (Beecham), WO 93/07147 (SmithKline Beecham), WO 94/10174 (SmithKline Beecham), WO 96/02537 (SmithKline Beecham) and EP 0200444 (Beecham) also disclose classes of fused bicyclic heterocyclic compounds as 5-HT receptor modulators.
WO 01/58869 (Bristol Myers Squibb) discloses a number of indazole-3- carboxamide derivatives as cannabinoid receptor antagonists.
WO 02/20484 (Astra Zeneca) discloses a broad class of compounds, including compounds containing an indazole group, as modulators of chemokine receptor activity. No indazoles are exemplified however. .
WO 02/053534 (Daichii) discloses a class of carboxylic acids and their esters as NLA inhibitors. The compounds, which are stated to be useful in the treatment of various disease states including inflammatory conditions, can comprise a halogenated phenyl acetic acid moiety linked to an indazole-3-carboxamido group.
WO 93/01169 (Merck) describes a class of compounds that have tachykinin receptor antagonist activity. The compounds may contain an indazole group, but there are no examples of indazole-3-carboxamides.
WO 98/03494 (Neurogen) discloses a class of 1 -phenyl- 1-piperazino-cycloalkanes and aza-cycloalkanes in which the phenyl group can form part of an indazole-3- carboxylic acid phenylamide. The compounds are disclosed as being capable of binding to mammalian neuropeptide Yl.
WO 99/29661 (Astra) describes a broad class of adamantane derivatives and oxa- adamantane derivatives as being useful in the treatment of rheumatoid arthritis, osteoarthritis, psoriasis and the growth and metastasis of malignant cells. However, there are no examples of indazoles.
WO 01/57024 (University College) discloses the use of various compounds, including indazoles, for blocking voltage dependent sodium channels.
WO 01/83472 (Acadia) describes a class of bicyclic heteroaryl compounds as muscarinic agonists. One ofthe exemplified compounds is the l-butyl-4- piperidinomethyl amide of indazole-3 -carboxylic acid.
EP 01013276 (Pfizer) discloses a class of compounds as modulators of chemokine activity that can be used in the treatment of inflammatory conditions. Indazoles are amongst the large list of compounds mentioned but there are no examples of indazoles.
WO 02/16318 (Pacific Corporation) discloses vanilloid receptor modulators for the treatment of inflammatory diseases. The modulator compounds can be indazoles but there is no disclosure of indazole-3-carboxamides.
WO 02/059112 (Vertex) discloses pyrazoles as protein kinase inhibitors but there are no examples of indazole-3-carboxamides.
WO 99/49856 (Genentech) discloses compounds that are useful in treating CD11/CDl 8 adhesion receptor mediated disorders such as inflammation, psoriasis and rheumatoid artliritis. The compounds can contain an indazole unit but there are no examples of indazole-3 -carboxamides.
JP 01117882 (Dainippon) discloses heteroarylamides for use in treating certain disorders ofthe gastrointestinal system. JP 11130750 (Fujisawa) discloses a class of arylamides for use in the treatment of CNS disorders.
WO 00/18738 (Zeneca) discloses a class of bis-amidophenyl compounds that act as inhibitors of cytokine production and which are stated to be useful in the treatment of inflammatory and allergic disease states. The compounds can contain an indazole unit but there are no examples of indazoles.
Kaneko et al. Nippon Shashin Gakkaishi 1995, 58(2), 122-8 discloses the use of indazole-3 -carboxylic acid phenylamide as a cyan dye forming compound.
Duykina et al, ZH. Obsh. Khim. 32, 81-84 (1962) discloses various indazole derivatives, including indazole-3 -carboxylic acid 4-methylbenzylamide.
Hannig et al. Pharmazie, 28, 11/12, 720-723 (1973) describes a number of 5- methylindazole-3 -carboxylic acid phenylamides and benzylamides as anti- inflammatory agents.
Schaus et al, J. Med. Chem., 41, 1943-1955 (1998) disclose a number of indazole- 3-carboxamides as 5-HT4 receptor antagonists.
Nagarajan et al, Proc. Indian Acad. Sci., 86A, 25-39 (1977) describes the synthesis of indazole-3 -carboxylic acid methoxyphenylamide.
Peter etal, Acta Pharmaceutica Hungarica, 43, 147-151 (1973) describes the preparation of a class of indazole-3 -carboxylic acid phenylalkylamides.
Summary of the Invention
The invention provides compounds that have cyclin dependent kinase inhibiting or modulating activity, and which it is envisaged will be useful in preventing or treating disease states or conditions mediated by the cyclin dependent kinases. Accordingly, in one aspect, the invention provides a compound ofthe formula (I) as defined herein for use in the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
The invention also provides the use of a compound ofthe formula (I) as defined herein for the manufacture of a medicament for the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
In a further aspect, the invention provides a method for the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase, which method comprises administering to a subject in need thereof a compound ofthe formula (I) as defined herein.
This invention also provides a method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, which method comprises administering to the mammal a compound ofthe formula (I) as defined herein in an amount effective in inhibiting abnormal cell growth.
This invention further provides a method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, the method comprising administering to the mammal a compound ofthe formula (I) as defined herein in an amount effective to inhibit CDK2 activity.
In another aspect, the invention provides a method of inhibiting a cyclin dependent kinase, which method comprises contacting the kinase with a kinase-inhibiting compound ofthe formula (I) as defined herein.
The invention further provides a method of modulating a cellular process (for example cell division) by inhibiting the activity of a cyclin dependent kinase using a compound ofthe formula (I) as defined herein.
The compounds ofthe invention are represented by the general formula (I):
Figure imgf000013_0001
wherein
A is a group R2 or CH -R2 where R2 is a carbocyclic or heterocyclic group having from 3 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members; R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra- Rb wherein Ra is a bond, O, CO, XJC(X2), C(X2)X1, X^CX^X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Ci_ hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the d-s hydrocarbyl group may optionally be replaced by O, S, SO, SO , NRC, XJC(X2), C(X2)XJ or X'CQ ^X1;
Rc is hydrogen or C^ hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC; R7 is selected from hydrogen and a C^s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Cι-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), C(X2)Xl or X1C(X2)X1;
R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members; R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N-[(morpholin-4-yl)phenyl-lH-indazole-3- carboxamide and N-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide.
9 9 9 The group A is a group R or CH2-R where R is a carbocyclic or heterocyclic group having from 3 to 12 ring members. In one particular embodiment, A is a group R2.
References to "carbocyclic" and "heterocyclic" groups as used herein, either with regard to the group R2 or any other substituent group, unless the context indicates otherwise include both aromatic and non-aromatic ring systems. Thus, for example, the term "carbocyclic and heterocyclic groups having from 3 to 12 ring members" includes within its scope aromatic, non-aromatic, unsaturated, partially saturated and fully saturated carbocyclic and heterocyclic ring systems.
The carbocyclic or heterocyclic groups can be aryl or heteroaryl groups having from 5 to 12 ring members, more usually from 5 to 10 ring members. The term "aryl" as used herein refers to a carbocyclic group having aromatic character and the term "heteroaryl" is used herein to denote a heterocyclic group having aromatic character. The terms "aryl" and "heteroaryl" embrace polycyclic (e.g. bicyclic) ring systems wherein one or more rings are non-aromatic, provided that at least one ring is aromatic. The aryl or heteroaryl groups can be monocyclic or bicyclic groups and can be unsubstituted or substituted with one or more substituents, for example one or more groups R10 as defined below.
Examples of heteroaryl groups are monocyclic and bicyclic groups containing from five to twelve ring members, and more usually from five to ten ring members. The heteroaryl group can be, for example, a five membered or six membered monocyclic ring or a bicyclic structure formed from fused five and six membered rings or two fused six membered rings. Each ring may contain up to about four heteroatoms typically selected from nitrogen, sulphur and oxygen. Typically the heteroaryl ring will contain up to 3 heteroatoms, more usually up to 2, for example a single heteroatom. In one embodiment, the heteroaryl ring contains at least one ring nitrogen atom. The nitrogen atoms in the heteroaryl rings can be basic, as in the case of a pyrazole, imidazole or pyridine, or essentially non-basic as in the case of an indole or pyrrole nitrogen. In general the number of basic nitrogen atoms present in the heteroaryl group, including any amino group substituents ofthe ring, will be less than five.
Examples of heteroaryl groups include but are not limited to pyridyl, pyrrolyl, furanyl, thiophenyl, imidazolyl, oxazolyl, oxadiazolyl, oxatriazolyl, isoxazolyl, thiazolyl, isothiazolyl, pyrazolyl, pyrazinyl, pyrimidinyl, pyridazinyl, triazinyl, triazolyl, tetrazolyl, quinolinyl, isoquinolinyl, benzfuranyl, benzthiophenyl, chromanyl, thiochromanyl, benzimidazolyl, benzoxazolyl, benzisoxazole, benzthiazolyl and benzisothiazole, isobenzofuranyl, isoindolyl, indolizinyl, indolinyl, isoindolinyl, purinyl (e.g., adenine, guanine), indazolyl, benzodioxolyl, chromenyl, isochromenyl, chroman, isochromanyl, benzodioxanyl, quinolizinyl, benzoxazinyl, benzodiazinyl, pyridopyridinyl, quinoxalinyl, quinazolinyl, cinnolinyl, phthalazinyl, naphthyridinyl and pteridinyl.
In the context ofthe group R2, one particular sub-group of compounds ofthe formula (I) is the group wherein R2 is selected from pyridyl, quinolinyl, isoquinolinyl and thiadiazolyl.
The pyridyl group can be a 2-pyridyl, 3-pyridyl or 4-pyridyl group but preferably it is a 3-pyridyl group.
Examples of carbocyclic aryl groups include phenyl, naphthyl, indenyl, and tetrahydronaphthyl. In the context ofthe group R2, preferred aryl groups are groups based on a benzene ring. Thus it may be, for example, a phenyl group which has no substituents other than the group B, or has one or more further substituents R10 as defined herein.
Examples of non-aromatic heterocyclic groups are groups having from 3 to 12 ring members, more usually 5 to 10 ring members. Such groups can be monocyclic or bicyclic, for example, and typically have from 1 to 5 heteroatom ring members (more usually 1, 2, 3 or 4 heteroatom ring members), usually selected from nitrogen, oxygen and sulphur. The heterocylic groups can contain, for example, cyclic ether moieties (e.g as in tetrahydrofuran and dioxane), cyclic thioether moieties (e.g. as in tetrahydrothiophene), cyclic amine moieties (e.g. as in pyrrolidine), cyclic amides (such as a pyrrolidinone, piperidone or caprolactam), cyclic sulphonamides (such as an isothiazolidine 1,1 -dioxide, [l,2]thiazinane 1,1- dioxide or [l,2]thiazepane 1,1 -dioxide), cyclic sulphones (e.g. as in sulpholane and sulpholene)), cyclic sulphoxides, and combinations thereof.
Particular examples include morpholine, piperidine, pyrrolidine, pyrrolidone, tetrahydrofuran, tetrahydrothiophene, dioxan, tetrahydropyran, imidazoline, imidazolidinone, oxazoline, thiazoline, piperazine, and N-alkyl piperazines such as N-methyl piperazine. In general, preferred non-aromatic heterocyclic groups include tetrahydrofuran, morpholine, piperazine, piperidine, pyrrolidine and pyrrolidone.
The carbocyclic and heterocyclic groups can be polycyclic fused ring systems but it is preferred that they are not bridged ring systems such as bicycloalkanes, tricycloalkanes and their oxa- and aza analogues (e.g. adamantane and oxa- adamantane). For an explanation ofthe distinction between fused and bridged ring fh systems, see Advanced Organic Chemistry, by Jerry March, 4 Edition, Wiley Interscience, pages 131-133, 1992.
The carbocyclic and heterocyclic groups can each be unsubstituted or substituted by one or more substituent groups R10 in addition to the group B-R1. For example, the carbocyclic and heterocyclic groups can be unsubstituted or substituted by 1, 2, 3 or 4 substituents. Where the carbocyclic or heterocyclic group is monocyclic or bicyclic, typically it is unsubstituted or has 1, 2 or 3 substituents, preferably 0, 1 or 2 substituents, and more preferably 0 or 1 substituent. In one embodiment, the carbocyclic and heterocyclic groups have no substituents in addition to the group B- R1.
The group R10 is selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO,
Figure imgf000017_0001
X^CX^X1, S, SO, SO2, NRC, SO2NR° or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C1-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe Cμs hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NR°, X1C(X2), C(X2)X! or X1C(X2)X1; Rc is hydrogen or CM hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC.
Where the substituent group R10 comprises or includes a carbocyclic or heterocyclic group, the said carbocyclic or heterocyclic group may be unsubstituted or may itself be substituted with one or more further substituent groups R10. In one sub-group of compounds ofthe formula (I), such further substituent groups R may include carbocyclic or heterocyclic groups. In another sub-group of compounds ofthe formula (I), the said further substituents do not include carbocyclic or heterocyclic groups but are otherwise selected from the groups listed above in the definition of R10.
In one general embodiment, the substituent groups R10 may be selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, amino; a group R -R wherein R is a bond, O, CO, XλC(X2),
Figure imgf000017_0002
S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen and a Ci-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C1.4 hydrocarbylamino and wherein one or more carbon atoms ofthe C^s hydrocarbyl group may optionally be replaced by O, S, SO, SO , NRC, X!C(X2), C(X2)X! or X1C(X2)X1; R° is hydrogen or CM hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC.
Examples of halogen substituents include fluorine, chlorine, bromine and iodine. Fluorine and chlorine are particularly preferred.
In the definition ofthe compounds ofthe formula (I) above and as used hereinafter, the term "hydrocarbyl" is a generic term encompassing aliphatic, alicyclic and aromatic groups having an all-carbon backbone, except where otherwise stated. Examples of such groups include alkyl, cycloalkyl, cycloalkenyl, carbocyclic aryl, alkenyl, alkynyl, cycloalkylalkyl, cycloalkenylalkyl, and carbocyclic aralkyl, aralkenyl and aralkynyl groups. Such groups can be unsubstituted or substituted by one or more substituents as defined herein. The examples and preferences expressed below apply to each ofthe hydrocarbyl substituent groups or hydrocarbyl-containing substituent groups referred to in the various definitions of substituents for compounds ofthe formula (I) unless the context indicates otherwise.
Generally by way of example, the hydrocarbyl groups can have up to eight carbon atoms, unless the context requires otherwise. Within the sub-set of hydrocarbyl groups having 1 to 8 carbon atoms, particular examples are Cι_6 hydrocarbyl groups, such as CM hydrocarbyl groups (e.g. C1-3 hydrocarbyl groups or d.2 hydrocarbyl groups), specific examples being any individual value or combination of values selected from , C2, C3, C , C5, C6, C and C8 hydrocarbyl groups.
The term "alkyl" covers both straight chain and branched chain alkyl groups. Examples of alkyl groups include methyl, ethyl, propyl, isopropyl, n-butyl, isobutyl, tert-butyl, n-pentyl, 2-pentyl, 3-pentyl, 2-methyl butyl, 3-methyl butyl, and n-heχyl and its isomers. Within the sub-set of alkyl groups having 1 to 8 carbon atoms, particular examples are C^ alkyl groups, such as CM alkyl groups (e.g. C1.3 alkyl groups or C1-2 alkyl groups).
Examples of cycloalkyl groups are those derived from cyclopropane, cyclobutane, cyclopentane, cyclohexane and cycloheptane. Within the sub-set of cycloalkyl groups the cycloalkyl group will have from 3 to 8 carbon atoms, particular examples being C3_6 cycloalkyl groups.
Examples of alkenyl groups include, but are not limited to, ethenyl (vinyl), 1- propenyl, 2-propenyl (allyl), isopropenyl, butenyl, buta-l,4-dienyl, pentenyl, and hexenyl. Within the sub-set of alkenyl groups the alkenyl group will have 2 to 8 carbon atoms, particular examples being C2-6 alkenyl groups, such as C2-4 alkenyl groups.
Examples of cycloalkenyl groups include, but are not limited to, cyclopropenyl, cyclobutenyl, cyclopentenyl, cyclopentadienyl and cyclohexenyl. Within the sub- set of cycloalkenyl groups the cycloalkenyl groups have from 3 to 8 carbon atoms, and particular examples are C3.6 cycloalkenyl groups.
Examples of alkynyl groups include, but are not limited to, ethynyl and 2-propynyl (propargyl) groups. Within the sub-set of alkynyl groups having 2 to 8 carbon atoms, particular examples are C2-6 alkynyl groups, such as C2.4 alkynyl groups.
Examples of carbocyclic aryl groups include substituted and unsubstituted phenyl.
Examples of cycloalkylalkyl, cycloalkenylalkyl, carbocyclic aralkyl, aralkenyl and aralkynyl groups include phenethyl, benzyl, styryl, phenylethynyl, cyclohexylmethyl, cyclopentylmethyl, cyclobutylmethyl, cyclopropylmethyl and cyclopentenylmethyl groups.
When present, a hydrocarbyl group can be optionally substituted by one or more substituents selected from hydroxy, oxo, alkoxy, carboxy, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, and monocyclic or bicyclic carbocyclic and heterocyclic groups having from 3 to 12 (typically 3 to 10 and more usually 5 to 10) ring members. Preferred substituents include halogen such as fluorine. Thus, for example, the substituent can be a partially fluorinated or perfluorinated group such as trifluoromethyl. In one embodiment preferred substituents include monocyclic carbocyclic and heterocyclic groups having 3-7 ring members.
One or more carbon atoms of a hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), C(X2)Xl or X1C(X2)X1 wherein X1 and X2 are as hereinbefore defined. For example, 1, 2, 3 or 4 carbon atoms ofthe hydrocarbyl group may be replaced by one ofthe atoms or groups listed, and the replacing atoms or groups may be the same or different. Examples of groups in which a carbon atom ofthe hydrocarbyl group has been replaced by a replacement atom or group as defined above include ethers and thioethers (C replaced by O or S), amides, esters, thioamides and thioesters (C replaced by X!C(X2) or C(X2)X1), sulphones and sulphoxides (C replaced by SO or SO2) and amines (C replaced by NRC).
Where an amino group has two hydrocarbyl substituents, they may, together with the nitrogen atom to which they are attached, and optionally with another heteroatom such as nitrogen, sulphur, or oxygen, link to form a ring structure of 4 to 7 ring members.
The definition "Ra-Rb" as used herein, either with regard to substituents present on the carbocyclic or heterocyclic moiety R2, or with regard to other substituents present at other locations on the compounds ofthe formula (I), includes inter alia compounds wherein Ra is selected from a bond, O, CO, OC(O), SC(O), NR°C(O), OC(S), SC(S), NRCC(S), OC(NRc), SC(NRC), NRCC(NR°), C(O)O, C(O)S, C(O)NR°, C(S)O, C(S)S, C(S) NRC, C(NRc)O, C(NR°)S, C(NRC)NR°, OC(O)O, SC(O)O, NRcC(O)O, OC(S)O, SC(S)O, NRcC(S)O, OC(NR°)O, SC(NRc)O, NRcC(NRc)O, OC(O)S, SC(O)S, NRcC(O)S, OC(S)S, SC(S)S, NRCC(S)S, OC(NRc)S, SC(NRC)S, NRCC(NRC)S, OC(O)NR°, SC(O)NRc, NRcC(O) NRC, OC(S)NR°, SC(S) NRC, NRCC(S)NRC, OC(NRc)NRc, SC(NRC)NRC, NRCC(NR°NRC, S, SO, SO2 , NRC, SO2NRc and NRQSO2 wherein Rc is as hereinbefore defined. The moiety Rb can be hydrogen or it can be a group selected from carbocyclic and heterocyclic groups having from 3 to 12 ring members (typically 3 to 10 and more usually from 5 to 10), and a Cι-8 hydrocarbyl group optionally substituted as hereinbefore defined.
Examples of hydrocarbyl, carbocyclic and heterocyclic groups are as set out above.
In one general embodiment, each substituent group R10, when present, is other than a carboxy group or a hydrocarbyl group terminated by a carboxy group or alkoxycarbonyl group.
In the compounds ofthe formula (I), B is a bond or an acyclic linker group. The linker group has a linking chain length of up to 3 atoms: in other words the number of atoms in the backbone ofthe linker group is 1, 2 or 3. Thus, for example, a group -CH2- has a linking chain length of one, whilst a group -CH2-CH2- has a linking chain length of two.
It is preferred that B is a bond or a linker group having a linking chain length of 1 atom.
The atoms making up the backbone ofthe linker group are selected from C, N, S and O, but preferably the atoms defining the linking chain length are all carbon atoms.
The linker group is typically a straight chain group. By "straight chain" is meant a group that has no branched side chains. In general a straight chain linker group may bear single atom substituents such as halogen and oxo, or substituents each of 1, 2 or 3 atoms, but would not usually have hydrocarbon substituents such as methyl, or larger multi-atom substituents each having four atoms or more such as methoxy or trifluoromethyl for example.
A preferred linker group B is a group (CH2)n wherein n is 1, 2 or 3, more preferably 1 or 2, and most preferably 1. The groups R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 7 ring members; a group Ra- Rb wherein Ra is a bond, O, CO, X1C(X2), C(X2)X1, X^OI^X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 (typically 3 to 10 and more usually 5 to 10) ring members, and a C1-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di- Cι-4 hydrocarbylamino, monocyclic carbocyclic and heterocyclic groups having from 3 to 12 (typically 3 to 10 and more usually 5 to 10) ring members and wherein one or more carbon atoms ofthe C^s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), CQ ^X1 or X^ ^X1;
Rc is hydrogen or C hydrocarbyl; and
X1 is O, S or NRC and X2 is =O, =S or =NRC.
It is preferred that R3 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and Ra-Rb.
More preferably R is hydrogen, Ct-6 alkyl, fluorine or chlorine, and most preferably R3 is hydrogen.
It is also preferred that R5 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and Ra-Rb.
More preferably R5 is hydrogen, d-6 alkyl, fluorine or chlorine, and most preferably R5 is hydrogen.
In one particular embodiment, R3 and R5 are both hydrogen.
It is preferred that R4 is selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members (typically 3 to 10 and more usually 5 to 10 ring members), and a group Ra-Rb. More preferably, R4 is selected from hydrogen, halogen, a heterocyclic group and a group Ra-Rb wherein Ra is a bond, O, CO, X*C(X2), C(X2)X1, X1C(X2)X1, S, SO, SO2, NR°, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 5 to 10 ring members, and a Ci-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-Q-4 hydrocarbylamino, monocyclic carbocyclic and heterocyclic groups having from 5 to 10 ring members and wherein one or more carbon atoms ofthe Q-s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), C(X2)XJ or X1C(X )X1.
Within the above definition of preferred groups R4, one particular group of compounds is the group in which R4 is selected from hydrogen, halogen, a heterocyclic group, a group O-Het where Het is a heterocyclic group having from 5 to 10 ring members, Cj.6 alkyl, Cι-6 alkoxy, C(O)NRcRb and SO2NRcRb wherein Rb is hydrogen or C^ alkyl.
R6 is preferably selected from hydrogen, methyl, amino, fluorine and chlorine, and more preferably hydrogen and amino. Most preferably, R6 is hydrogen.
In one particular group of compounds ofthe formula (I), R , R and R each are hydrogen.
In one general embodiment ofthe invention, the compounds ofthe formula (I) may be such that when R1 is SO2NR7R8, neither of R7 and R8 is a Ci-8 hydrocarbyl group in which the carbon atom attached to the nitrogen atom ofthe group SO2NR7R8 is substituted by an oxo group.
In another general embodiment, the compounds ofthe formula (I) may be such that R1 is other than the heterocyclic group N-morpholino when B is a bond and A is R2 wherein R2 is aryl.
Novel Compounds Many ofthe compounds ofthe formula (I) are novel. Accordingly, in another aspect, the invention provides a compound ofthe formula (II):
Figure imgf000024_0001
wherein E is a group R12 or CH2-R12a where R12 is a substituted or unsubstituted, non-bridged, carbocyclic or heterocyclic group having from 3 to 12 ring members, other than a diazacycloalkyl moiety, and R12a is an unsubstituted or substituted aryl or heteroaryl group having from 5 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra- Rb wherein Ra is a bond, O, CO, X1C(X2), C(X2)Xl, X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NR°SO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Cι-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the -s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^CX ), C(X2)Xl ov XlC(X2)Xl;
Rc is hydrogen or C hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC; R7 is selected from hydrogen and a d-s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-d.4 hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Q-δ hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^OX2), CO ^X1 or X^C ^X1;
R is selected from R and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR R or NR R may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for the groups R12 and R12a can be one or more substituent groups R ° selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, XJC(X2), C(X2)X1, X^CX^X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a Cι-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-Cw hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe Ci-s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X!C(X2), C(X2)X* or X^O^X1;
Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC; with the provisos that:
(a) when R12 is an azacycloalkyl or diazacycloalkyl group, at least one nitrogen atom ofthe azacycloalkyl or diazacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group;
(b) when E is a substituted phenyl group, the or each substituent is other than a 5-7 membered non-aromatic ring (such as cyclohexyl) having attached thereto a diazacycloalkyl moiety (such as piperazine), a nitrogen atom of which moiety bears an aryl or heteroaryl substituent; and
(c) R12 and R12a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
(i) N- [(morpholin-4-yl)phenyl- 1 H-indazole-3 -carboxamide ;
(ii) N-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide;
1 7 0
(iii) compounds wherein E is phenyl, R is NR R and B is a group
-CH(CH2OH)CH2-; (iv) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy;
(v) compounds wherein R to R are all hydrogen, E is unsubstituted pyridyl or pyridylmethyl, B is a bond and R1 is hydrogen;
(vi) compounds wherein E is phenyl substituted with one or more of alkyl, alkoxy, alkylsulphanyl, alkylsulphinyl other than røetø-alkylsulphinyl, alkylsulphonyl other than met -alkylsulphonyl, halogen, nitro and trihalomethyl, B is a bond, and R1 is hydrogen;
(vii) compounds wherein E is a thiophene group bearing a 3-aminocarbonyl substituent; (viii) the compound wherein E is unsubstituted phenyl orpαrα-methoxyphenyl, and each of R to R is hydrogen;
(ix) N-4-methylbenzyl- 1 H-indazole-3-carboxamide;
(x) compounds wherein R3, R5 and R6 are each hydrogen, R4 is methyl and A is unsubstituted benzyl, unsubstituted phenyl, methylphenyl, meta- trifluoromethylphenyl, and ort/zø-mefhoxyphenyl;
(xi) compounds in which E is a 2,2-dimethyl-l ,3 -dioxane ring;
(xii) compounds containing a benzene ring substituted by a pair of wet -oriented carboxamido moieties;
(xiii) compounds wherein E is a trisubstituted phenyl group and two ofthe substitutents are fluoro and chloro respectively. In one embodiment, E-B-R1 may be other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
In another embodiment, E-B-R1 may be other than a saturated azabicyclic moiety or an imidazolyl moiety.
In another general embodiment, the compound ofthe formula (II) is other than one in which E is unsubstituted pyridyl or pyridylmethyl, B is a bond and R1 is hydrogen.
In a further embodiment, when E-B-R1 is an unsubstituted phenyl group, R3 to R6 are other than a group Ra-Rb wherein Ra is a bond and Rb is a substituted C3-C3 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
The invention also provides a group of novel compounds ofthe formula (III):
Figure imgf000027_0001
wherein G is a group R14 or CH2-R14 where R14 is a carbocyclic group having from 3 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R13 is a group selected from SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra- Rb wherein Ra is a bond, O, CO, XlC(X2), C(X2)Xl, X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Q.s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Ct-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C ^X1 or X^f ^X1;
Rc is hydrogen or CM hydrocarbyl; X1 is O, S or NR° and X2 is =O, =S or =NRC;
R7 is selected from hydrogen and a C\.s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the C s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), C(X2)XJ or X^CX^X1;
R is selected from R and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N-[(morpholin-4-yl)phenyl-lH-indazole-3- carboxamide andN-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide; and further excluding; (i) compounds wherein G is phenyl, R1 is NR7R8 and B is a group - CH(CH2OH)CH2-;
(ii) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy.
One sub-group of novel compounds ofthe invention is represented by the general formula (IV):
Figure imgf000029_0001
wherein R3 to R8, G and B are as hereinbefore defined.
Within the sub-group of compounds ofthe formula (IN), preferred compounds include those wherein G is a group R14 wherein R14 is an aryl group having six ring members and B is a bond or a methylene group.
Another preferred group of compounds within formula (IN) is the group of
7 8 7 compounds in which R and R are selected from hydrogen and CM alkyl or R and R8 together with the nitrogen atom form a saturated five or six membered heterocyclic ring having one or two heteroatoms.
7 R Examples of such compounds include compounds wherein R and R together with the nitrogen atom form a saturated heterocyclic ring selected from morpholino, piperidino, piperazino and pyrrolidino.
7 R
Further particular examples are compounds in which R is hydrogen and R is hydrogen or methyl.
Another group of novel compounds ofthe invention is represented by the general formula (N):
Figure imgf000029_0002
wherein R3 to R8, G and B are as hereinbefore defined.
Within the sub-group of compounds ofthe formula (N), preferred compounds include those wherein G is a group R14 wherein R14 is an aryl group having six ring members and B is a bond or a methylene group.
A further novel group of compounds ofthe invention is represented by the general formula (Nl):
Figure imgf000030_0001
wherein R3 to R6 and G are as hereinbefore defined and Het' is a heterocylic group having from 3 to 7 ring members, but excluding the compound Ν-[(morpholin-4- yl)phenyl] - 1 H-indazole-3 -carboxamide .
Within the sub-group of compounds ofthe formula (VI), preferred compounds include those wherein G is a group R14 wherein R14 is an aryl group having six ring members and B is a bond or a methylene group.
In one sub-group of compounds ofthe formula (VI), a carbon atom ofthe heterocyclic group Het' is linked to the group G.
The group Het' can be, for example, a five membered heteroaryl ring containing 2 or more nitrogen ring members. Examples of such groups include tetrazolyl, pyrrolidonyl (e.g.N-pyrrolidonyl), oxazolyl and imidazolyl.
A further sub-group of novel compounds ofthe invention is represented by the formula (VII):
Figure imgf000031_0001
wherein R >3 + to. R , R , G and B are as hereinbefore defined.
Within the sub-group of compounds ofthe formula (Nil), typically G is a group R14 wherein R14 is an aryl group having six ring members and B is a bond or a methylene group, preferably a methylene group.
Preferred compounds ofthe formula (Nil) are those wherein R7 is selected from hydrogen and C alkyl and R9 is selected from hydrogen, CM alkyl and C1.4 alkanoyl such as acetyl.
Another group of novel compounds ofthe invention is defined by formula (NIII):
Figure imgf000031_0002
wherein R3 to R6 and Rb are as hereinbefore defined and R11 represents hydrogen or one or more substituents selected from halogen, C alkyl, CM alkoxy, trifluoromethyl and trifluoromethoxy.
In one embodiment, the group SO2Rb is attached to the røetø-position ofthe benzene ring. In another embodiment, the group SO2R is attached to the j?αrø-position ofthe benzene ring.
Preferred compounds are those in which R11 is hydrogen.
In one group of compounds ofthe formula (NIII), R is C alkyl, preferably methyl.
In another aspect, the invention provides a compound ofthe formula (IX):
Figure imgf000032_0001
wherein
R to R and B are as hereinbefore defined; J is a group R15 or CH2-R15a where R15 is a substituted or unsubstituted, non- bridged heterocyclic group having from 5 to 12 ring members, other than a diazacycloalkyl moiety, and R15a is an unsubstituted or substituted aryl or heteroaryl group having from 5 to 12 ring members;
R1 is hydrogen when R15a is aryl or, when R15a is other than aryl, R1 is hydrogen or a group selected from SO2Rb, SO2ΝR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members; and the optional substituents for the groups R15 and R15a can be one or more substituent groups R10 selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2), C(X2)X!, X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C^s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe Cι-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C X^X1 or X^C ^X1; provided that when R15a is aryl it is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O, by a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members; Rc is hydrogen or CM hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC; with the provisos that: (a) when R15 is an azacycloalkyl group and all of R3 to R6 are hydrogen, at least one nitrogen atom ofthe azacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group;
(b) R15 and R15a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
(i) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy;
(ii) compounds wherein R3 to R6 are all hydrogen, J is unsubstituted pyridyl or pyridylmethyl- B is a bond and R1 is hydrogen; (iii) compounds wherein J is phenyl substituted with one or more of alkyl, alkoxy, alkylsulphanyl, alkylsulphinyl other than etα-alkylsulphinyl, alkylsulphonyl other than meto-alkylsulphonyl, halogen, nitro and trihalomethyl, B is a bond, and R1 is hydrogen;
(iv) compounds wherein J is a thiophene group bearing a 3-aminocarbonyl substituent;
(v) the compound wherein J is unsubstituted phenyl or pαra-mefhoxyphenyl, and each of R3 to R6 is hydrogen;
(vi) N-4-methylbenzyl-lH-indazole-3-carboxamide; (vii) compounds wherein R3, R5 and R6 are each hydrogen, R4 is methyl and A is unsubstituted benzyl, unsubstituted phenyl, methylphenyl, meta- trifluoromethylphenyl, and ørt zø~methoxyphenyl;
(viii) compounds in which J is a 2,2-dimethyl-l ,3-dioxane ring;
(ix) compounds containing a benzene ring substituted by a pair of weto-oriented carboxamido moieties; and
(x) compounds wherein J is a trisubstituted phenyl group and two ofthe substituents are fluoro and chloro respectively.
The invention also provides a group of novel compounds ofthe formula (X):
Figure imgf000034_0001
wherein
L is a group R16 or CH2-R16 where R16 is a substituted or unsubstituted heteroaryl group other than imidazole, the heteroaryl group having from 5 to 12 ring members, at least one of which is nitrogen;
R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
B, R3, R4, R5 and R6 are as hereinbefore defined, provided that R4 and R5 cannot both be methoxy; and the optional substituents for R16 can be one or more substituent groups R10 as hereinbefore defined; but excluding compounds wherein all of R3 to R6 are hydrogen and L-B-R1 defines an unsubstituted pyridyl or pyridylmethyl group.
In one general embodiment, the compound ofthe formulae (IX) or (X) may be other than a compound in which J is unsubstituted pyridyl or pyridylmethyl, B is a bond and R1 is hydrogen. Within the general formula (X), one sub-group of compounds is represented by the formula (XI):
Figure imgf000035_0001
in which R17 is hydrogen, B-R1 or R10, and wherein R4, B-R1 and R10 are as
A 17 hereinbefore defined, provided that at least one of R and R is other than hydrogen.
A preferred sub-group of compounds within formula (XI) can be represented by the formula (XII):
Figure imgf000035_0002
Another sub-group of compounds within the formula (X) is represented by the formula (XIII):
Figure imgf000035_0003
in which R17 is hydrogen, B-R1 or R10, and wherein R4, B-R1 and R10 are as hereinbefore defined. A further sub-group of compounds within the formula (X) is represented by the formula (XIV):
Figure imgf000036_0001
in which R17 is hydrogen, B-R1 or R10, and wherein R4, B-R1 and R10 are as hereinbefore defined.
Another group of novel compounds ofthe invention is the group of compounds of the formula (XN):
Figure imgf000036_0002
wherein
90 90 90 M is a group R or CH2-R where R is an aryl group having from 6 to 12 ring members and being optionally substituted by one or two substituent groups R10 which may be the same or different;
R18 is selected from hydrogen, halogen, and carbocyclic and heterocyclic groups having from 3 to 12 ring members; R19 is selected from hydrogen and amino, provided that at least one of R18 and R19 is other than hydrogen;
90 provided that the aryl group R is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms selected from C, Ν, S and O, by a group selected from SO2Rb, SO2ΝR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members. Preferred compounds ofthe formula (XN) are those wherein R18 is halogen, especially iodine or chlorine, and R19 is hydrogen.
Another group of novel compounds ofthe invention is the group of compounds of the formula (XNI):
Figure imgf000037_0001
wherein
R3 to R6 are as hereinbefore defined;
Q is an optionally substituted non-bridged non-aromatic heterocyclic group having from 5 to 7 ring members of which at least one is a nitrogen atom, the group being other than a diazacycloalkyl group; and the optional substituents for the group Q can be one or more (preferably up to 2, for example 1) substituent groups R21 selected from SO2Rb, SO2ΝR7R8, CONR7R8, NR7R9, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra- Rb wherein Ra is a bond, O, CO, X!C(X2),
Figure imgf000037_0002
X1C(X2)X1, S, SO, SO2, NRC, SO NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a -s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C1.4 hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Cι-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), C(X2)X! or X1C(X2)X1;
Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC; provided that when Q is an azacycloalkyl group and R3 to R6 are all hydrogen, at least one nitrogen atom ofthe azacycloalkyl or diazacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group.
In each ofthe groups of novel compounds (II) to (XNI), it is preferred that the compounds do not contain a benzene ring substituted by a pair of meta-oήented carboxamido moieties.
In the compounds ofthe formulae (IX) and (X), it is preferred that J-B-R1 and L-B- R1 are other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
In the compounds ofthe formulae (IX), (X) and (XNI), it is preferred that J-B-R1 and L-B-R1 are other than a saturated azabicyclic moiety or an imidazolyl moiety.
In compounds ofthe formulae (IX) and (XIN), it is preferred that when J-B-R1 is an unsubstituted phenyl group, R3 to R6 are each other than a group Ra-Rb wherein Ra is a bond and Rb is a substituted C3-C8 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
In the foregoing definitions of novel compounds ofthe invention, the groups E, G, J and L are sub-groups ofthe group A defined in relation to compounds ofthe formula (I). Similarly, the groups R12, R12a and R14 are sub-groups ofthe group R2, and the group R13 is a sub-group ofthe group R1. Unless the context requires otherwise, the general and specific preferences, embodiments and examples set out above in relation to A, R1 and R2, apply also to the sub-groups E, G, R13, R12, R12a and R14.
The novel compounds ofthe formulae (IX) to (XNI) defined above are sub-groups ofthe formula (I). Except where the context dictates otherwise, the general and specific definitions of substituent groups, and the general and specific definitions, preferences and examples set out for each ofthe moieties R1 to R10, A and B apply also to compounds ofthe formulae (IX) to (XNI). For the avoidance of doubt, it is to be understood that each general and specific preference, embodiment and example ofthe groups R1 may be combined with each general and specific preference, embodiment and example ofthe groups R2 and/or R3 and/or R4 and/or R5 and/or R6 and/or R7 and/or R8 and/or R9 and/or R10 and/or A and/or B and their associated sub-groups, and that all such combinations are embraced by this application.
The various functional groups and substituents making up the compounds ofthe formula (I) are typically chosen such that the molecular weight ofthe compound of the formula (I) does not exceed 1000. More usually, the molecular weight ofthe compound will be less than 750, for example less than 700, or less than 650, or less than 600, or less than 550. More preferably, the molecular weight is less than 525 and, for example, is 500 or less.
Particular novel compounds ofthe invention are as described in the Examples below.
Specific novel compounds of the invention include : lH-Indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)-amide; lH-Indazole-3 -carboxylic acid [3-(lH-tetrazol-5-yl)-phenyl]-amide;
1 H-Indazole-3-carboxylic acid [4-(acetylamino-methyl)-phenyl] -amide; lH-Indazole-3-carboxylic acid [4-(2-oxo-pyrrolidin-l-yl)-phenyl]-amide; lH-Indazole-3 -carboxylic acid (3-oxazol-5-yl-phenyl)-amide; lH-Indazole-3-carboxylic acid [4-(lH-imidazol-4-yl)-phenyl]-amide; lH-Indazole-3-carboxylic acid (3-methanesulphonyl-phenyl)-amide; lH-Indazole-3 -carboxylic acid [4-(morpholine-4-sulphonyl)-phenyl]-amide;
5 -Iodo-lH-indazole-3 -carboxylic acid (4-sulphamoyl-phenyl)-amide; 5-Iodo-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)-amide;
5-Iodo-lH-indazole-3-carboxylic acid (3-methanesulphonyl-phenyl)-amide;
5-Iodo-lH-indazole-3-carboxylic acid [4-(acetylamino-methyl)-phenyl]-amide;
5 -nitro- lH-indazole-3 -carboxylic acid (4-sulphamoyl-phenyl)-amide;
5-nitro-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)-amide; 5-thiophen-2-yl-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide;
5-(3,5-dimethyl-isoxazol-4-yl)-lH-indazole-3-carboxylic acid (4- mefhylsulphamoylmethyl-phenyl)-amide; 5-furan-2-yl-l H-indazole-3 -carboxylic acid (4-methylsulphamoylmethyl -phenyl)- amide;
5-benzofuran-2-yl- lH-indazole-3 -carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide;
N-phenyl-5-iodo- 1 H-indazole-3-carboxamide; 5-morpholin-4-yl- lH-indazole-3-carboxylic acid phenylamide;
5-chloro- 1 H-indazole-3 -carboxylic acid (5-nitro-pyridin-2-yl)-amide; lH-indazole-3-carboxylic acid (4-sulphamoyl-phenyl)-amide;
5-thiophen-2-yl-lH-indazole-3-carboxylic cid (4-methylsulphamoylmethyl- phenyl)-amide; 5-thiazol-2-yl-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide;
4- [(5-iodo- 1 H-indazole-3 -carbonyl)-amino] -piperidine- 1 -carboxylic acid ethyl ester;
1 H-indazole-3 -carboxylic acid [4-(thiazol-2-ylsulphamoyl)-phenyl] -amide; 5-phenyl-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid [4-(methanesulphony lamino-methyl)- phenyl] -amide;
4-[(5-nitro-lH-indazole-3-carbonyl)-amino]-piperidine-l-carboxylic acid ethyl ester;
5 -chloro- 1 H-indazole-3 -carboxylic acid ( 1 -benzyl-pyrrolidin-3 -yl)-amide;
4- [(5-chloro- 1 H-indazole-3 -carbonyl)-amino] -piperidine- 1 -carboxylic acid ethyl ester;
5-iodo- lH-indazole-3-carboxylic acid (6-methoxy-pyridin-3-yl)-amide; 5-iodo-lH-indazole-3-carboxylic acid pyridin-3-yl-amide;
5-iodo- lH-indazole-3-carboxylic acid quinolin-3-ylamide; 5-iodo-lH-indazole-3-carboxylic acid (tetrahydro-pyran-4-yl)-amide;
5 -chloro- 1 H-indazole-3 -carboxylic acid ( 1 -methy l-piperidin-4-yl)-amide;
5-iodo-lH-indazole-3-carboxylic acid (2-chloro-pyridin-3-yl)-amide;
5-chloro-lH-indazole-3-carboxylic acid benzylamide; 5-chloro-lH-indazole-3-carboxylic acid 4-(4-methyl-piperazin-l-yl)-benzylamide;
5-chloro-lH-indazole-3-carboxylic acid pyridin-3-ylamide;
5-iodo- 1 H-indazole-3 -carboxylic acid (6-cyano-pyridin-3-yl)-amide;
5-chloro- lH-indazole-3-carboxylic acid phenylamide;
5-iodo-lH-indazole-3-carboxylic acid (6-methyl-pyridazin-3-yl)-amide; 5-chloro-lH-indazole-3-carboxylic acid (5-ethyl-[l,3,4]thiadiazol-2-yl)-amide;
5-iodo-lH-indazole-3-carboxylic acid (4-morpholin-4-yl-ρhenyl)-amide;
5 -iodo-1 H-indazole-3 -carboxylic acid (2-oxo-l ,2-dihydro-pyridin-3-yl)-amide; lH-indazole-3-carboxylic acid (4-morpholin-4-yl-phenyl)-amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid phenylamide; 5-iodo-lH-indazole-3-carboxylic acid (6-chloro-pyridin-3-yl)-amide;
4-[(lH-indazole-3-carbonyl)-amino]-piperidine-l-carboxylic acid tert-butyl ester;
5-iodo-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide;
5 -iodo-1 H-indazole-3 -carboxylic acid (6-acetylamino-pyridin-3-yl)-amide;
5-amino-lH-indazole-3-carboxylic acid phenylamide; 5 -iodo-1 H-indazole-3 -carboxylic acid (4-methylaminosulphonylmethyl-phenyl)- amide;
5-amino-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenyl)-amide;
7-amino-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenyl)-amide;
5 - [3 -(2-chloro-ethyl)-ureido] - 1 H-indazole-3 -carboxylic acid (4-methy lsulphamoyl- methyl-phenyl)-amide;
5-nitro- lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)-amide;
5-amino- 1 H-indazole-3-carboxylic acid (4-methylsulphamoylmethyl -phenylamide;
5-iodo- 1 H-indazole-3 -carboxylic acid piperidin-4-ylamide 5-chloro- 1 H-indazole-3 -carboxylic acid [4-(acetylamino-methyl)-phenyl]-amide;
1 H-indazole-3 -carboxylic acid [l-(2,2,2 trifluoro-acetyl)-Piperidin-4- yl]-amide; lH-indazole-3-carboxylic acid piperidin-4-ylamide; lH-indazole-3-carboxylic acid (l-acetyl-piperidin-4-yl)-amide;
1 H-indazole-3 -carboxylic acid (1 -methanesulphonyl-piperidin-4-yl)-amide; lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide; 4-bromo-l H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide;
5-nitro-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide;
5-amino-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide;
5-amino-4-bromo-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide;
5 -methyl- 1 H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide; 6-bromo-l H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide;
5 -chloro- 1 H-indazole-3 -carboxylic acid (4-morpholin-4-yl-phenyl)-amide;
5-chloro-lH-indazole-3-carboxylic acid [3-(lH-tetrazol-5-yl)-phenyl]-amide;
5-iodo- 1 H-indazole-3 -carboxylic acid (4-pyrrolidin- 1 -ylmethyl-phenyl)-amide;
5-chloro-lH-indazole-3-carboxylic acid [4-(thiazol-2-ylsulphamoyl)-phenyl]- amide;
5-chloro- 1 H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide;
3 - [(5 -chloro- 1 H-indazole-3 -carbonyl)-amino] -pyrrolidine- 1 -carboxylic acid methyl ester;
5-fluoro-lH-indazole-3-carboxylic acid phenylamide; 5-morpholin-4-yl-lH-indazole-3-carboxylic acid (6-chloro-pyridin-3-yl)-amide;
1 H-indazole-3 -carboxylic acid (6-chloro-pyridin-3-yl)-amide;
5 -phenethyl-1 H-indazole-3 -carboxylic acid phenylamide;
5 -( 1 , 1 -dioxo- 1 lambda* 6* -isothiazolidin-2-yl)- 1 H-indazole-3 -carboxylic acid phenylamide; 5-biphenyl-2-yl-lH-indazole-3-carboxylic acid phenylamide;
5 -pyrrolidin-l-yl-1 H-indazole-3 -carboxylic acid phenylamide;
5 -chloro- 1 H-indazole-3 -carboxylic acid [5-(tetrahydro-furan-2-yl)-
[l,3,4]thiadiazol-2-yl]-amide and 5 -nitro- 1 H-indazole-3 -carboxylic acid (3-methanesulphonyl-phenyl)-amide. Pharmaceutical compositions comprising a novel compound as hereinbefore defined and a pharmaceutically acceptable carrier also form part ofthe invention.
The invention also provides a novel compound as hereinbefore defined for use in medicine, for example for one or more ofthe uses set out above in relation to compounds ofthe formula (I).
Many compounds ofthe formula (I) can exist in the form of salts, for example acid addition salts or, in certain cases salts of organic and inorganic bases such as carboxylate, sulphonate and phosphate salts. All such salts are within the scope of this invention, and references to compounds ofthe formula (I) include the salt forms of the compounds .
Acid addition salts may be formed with a wide variety of acids, both inorganic and organic. Examples of acid addition salts include salts formed with hydrochloric, hydriodic, phosphoric, nitric, sulphuric, citric, lactic, succinic, maleic, malic, isethionic, fumaric, benzenesulphonic, toluenesulphonic, methanesulphonic, ethanesulphonic, naphfhalenesulphonic, valeric, acetic, propanoic, butanoic, malonic, glucuronic and lactobionic acids.
If the compound is anionic, or has a functional group which may be anionic (e.g., -COOH may be -COO"), then a salt may be formed with a suitable cation. Examples of suitable inorganic cations include, but are not limited to, alkali metal ions such as Na+ and K+, alkaline earth cations such as Ca2+ and Mg2+, and other cations such as Al3+. Examples of suitable organic cations include, but are not limited to, ammonium ion (i.e., NH4 +) and substituted ammonium ions (e.g., NH3R+, NH2R2 +, NHR3 +, NR^. Examples of some suitable substituted ammonium ions are those derived from: ethylamine, diethylamine, dicyclohexylamine, triethylamine, butylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, benzylamine, phenylbenzylamine, choline, meglumine, and tromethamine, as well as amino acids, such as lysine and arginine. An example of a common quaternary ammonium ion is N(CH3) +. Where the compounds ofthe formula (I) contain an amine function, these may form quaternary ammonium salts, for example by reaction with an alkylating agent according to methods well known to the skilled person. Such quaternary ammonium compounds are within the scope of formula (I).
Compounds ofthe formula (I) containing an amine function may also form N- oxides. A reference herein to a compound ofthe formula (I) that contains an amine function also includes the N-oxide.
Where a compound contains several amine functions, one or more than one nitrogen atom may be oxidised to form an N-oxide. Particular examples of N- oxides are the N-oxides of a tertiary amine or a nitrogen atom of a nitrogen- containing heterocycle.
N-Oxides can be formed by treatment ofthe corresponding amine with an oxidizing agent such as hydrogen peroxide or a per-acid (e.g. a peroxycarboxylic acid), see for example Advanced Organic Chemistry, by Jerry March, 4th Edition, Wiley Interscience, pages. More particularly, N-oxides can be made by the procedure of L. W. Deady (Syn. Comm. 1977, 7, 509-514) in which the amine compound is reacted with w-chloroperoxybenzoic acid (MCPBA), for example, in an inert solvent such as dichloromethane.
Compounds ofthe formula may exist in a number of different geometric isomeric, and tautomeric forms and references to compounds ofthe formula (I) include all such forms. For the avoidance of doubt, where a compound can exist in one of several geometric isomeric or tautomeric forms and only one is specifically described or shown, all others are nevertheless embraced by formula (I).
Esters such as carboxylic acid esters and acyloxy esters ofthe compounds of formula (I) bearing a carboxylic acid group or a hydroxyl group are also embraced by Formula (I). Examples of esters are compounds containing the group -C(=O)OR, wherem R is an ester substituent, for example, a C1.7 alkyl group, a C3.20 heterocyclyl group, or a C5.20 aryl group, preferably a C1.7 alkyl group. Particular examples of ester groups include, but are not limited to, -C(=O)OCH3, -C(=O)OCH2CH3, -C(=O)OC(CH3)3, and -C(=O)OPh. Examples of acyloxy (reverse ester) groups are represented by -OC(=O)R, wherein R is an acyloxy substituent, for example, a C1-7 alkyl group, a C3.20 heterocyclyl group, or a C5.20 aryl group, preferably a Cjμ-7 alkyl group. Particular examples of acyloxy groups include, but are not limited to, -OC(=O)CH3 (acetoxy), -0C(O)CH2CH3, -OC(=O)C(CH3)3, -OC(=O)Ph, and -OC(=O)CH2Ph.
Also encompassed by formula (I) are any polymorphic forms ofthe compounds, solvates (e.g. hydrates), complexes (e.g. inclusion complexes or clathrates with compounds such as cyclodextrins, or complexes with metals) ofthe compounds, and pro-drugs ofthe compounds. By "prodrugs" is meant for example any compound that is converted in vivo into a biologically active compound ofthe formula (I).
For example, some prodrugs are esters ofthe active compound (e.g., a physiologically acceptable metabolically labile ester). During metabolism, the ester group (-C(=O)OR) is cleaved to yield the active drug. Such esters may be formed by esterification, for example, of any ofthe carboxylic acid groups (-C(=O)OH) in the parent compound, with, where appropriate, prior protection of any other reactive groups present in the parent compound, followed by deprotection if required.
Examples of such metabolically labile esters include those ofthe formula -
C(=O)OR wherein R is:
Ci.7a.kyl
(e.g., -Me, -Et, -nPr, -iPr, -nBu, -sBu, -iBu, -tBu);
C^aminoalkyl (e.g., aminoethyl; 2-(N,N-diethylamino)ethyl; 2-(4-morpholino)ethyl); and acyl0xy-Q.7a.kyl
(e.g., acyloxymethyl; acyloxyethyl; pivaloyloxymethyl; acetoxymethyl; 1-acetoxyethyl;
1 -(1 -methoxy- 1 -methyl)ethyl-carbonxyloxyethyl; 1 -(benzoyloxy)ethyl; isopropoxy-carbonyloxymethyl; 1 -isopropoxy-carbonyloxyethyl; cyclohexyl-carbonyloxymethyl; 1-cyclohexyl-carbonyloxyethyl; cyclohexyloxy-carbonyloxymethyl; 1-cyclohexyloxy-carbonyloxyethyl; (4-tetrahydropyranyloxy) carbonyloxymethyl; l-(4-tetrahydropyranyloxy)carbonyloxyethyl; (4-tetrahydropyranyl)carbonyloxymethyl; and 1 -(4-tetrahydropyranyl)carbonyloxyethyl) .
Also, some prodrugs are activated enzymatically to yield the active compound, or a compound which, upon further chemical reaction, yields the active compound (for example, as in ADEPT, GDEPT, LIDEPT, etc.). For example, the prodrug may be a sugar derivative or other glycoside conjugate, or may be an amino acid ester derivative.
Where the compounds ofthe formula (I) contain chiral centres, all individual optical forms such as enantiomers, epimers and diastereoisomers, as well as racemic mixtures ofthe compounds are within the scope of formula (I).
The compounds ofthe formula (I) are inhibitors of cyclin dependent kinases. As such, they are expected to be useful in providing a means of arresting, or recovering control of, the cell cycle in abnormally dividing cells. It is therefore anticipated that the compounds will prove useful in treating or preventing proliferative disorders such as cancers. It is also envisaged that the compounds ofthe invention will be useful in treating conditions such as viral infections, autoimmune diseases and neurodegenerative diseases for example.
CDKs play a role in the regulation ofthe cell cycle, apoptosis, transcription, differentiation and CNS function. Therefore, CDK inhibitors could be useful in the treatment of diseases in which there is a disorder of proliferation, apoptosis or differentiation such as cancer. In particular RB+ve tumours may be particularly sensitive to CDK inhibitors.
Examples of cancers which may be inhibited include, but are not limited to, a carcinoma, for example a carcinoma ofthe bladder, breast, colon (e.g. colorectal carcinomas such as colon adenocarcinoma and colon adenoma), kidney, epidermal, liver, lung, for example adenocarcinoma, small cell lung cancer and non-small cell lung carcinomas, oesophagus, gall bladder, ovary, pancreas e.g. exocrine pancreatic carcinoma, stomach, cervix, thyroid, prostate, or skin, for example squamous cell carcinoma; a hematopoietic tumour of lymphoid lineage, for example leukemia, acute lymphocytic leukemia, B-cell lymphoma, T-cell lymphoma, Hodgkin's lymphoma, non-Hodgkin's lymphoma, hairy cell lymphoma, or Burkett's lymphoma; a hematopoietic tumor of myeloid lineage, for example acute and chronic myelogenous leukemias, myelodysplastic syndrome, or promyelocytic leukemia; thyroid follicular cancer; a tumour of mesenchymal origin, for example fibrosarcoma or habdomyosarcoma, ; a tumor ofthe* central or peripheral nervous system, for example astrocytoma, neuroblastoma, glioma or schwannoma; melanoma; seminoma; teratocarcinoma; osteosarcoma; xenoderoma pigmentoum; keratoctanthoma; thyroid follicular cancer; or Kaposi's sarcoma.
CDKs are also known to play a role in apoptosis, proliferation, differentiation and transcription and therefore CDK inhibitors could also be useful in the treatment of the following diseases other than cancer; viral infections, for example herpes virus, pox virus, Epstein-Barr virus, Sindbis virus, adenovirus, HIN, HPN, HCN and HCMN; prevention of AIDS development in HIN-infected individuals; chronic inflammatory diseases, for example systemic lupus erythematosus, autoimmune mediated glomerulonephritis, rheumatoid arthritis, psoriasis, inflammatory bowel disease, and autoimmune diabetes mellitus; cardiovascular diseases for example cardiac hypertrophy, restenosis, atherosclerosis; neurodegenerative disorders, for example Alzheimer's disease, AIDS-related dementia, Parkinson's disease, amyotropic lateral sclerosis, retinitis pigmentosa, spinal muscular atropy and cerebellar degeneration; glomerulonephritis; myelodysplastic syndromes, ischemic injury associated myocardial infarctions, stroke and reperfusion injury, arrhythmia, atherosclerosis, toxin-induced or alcohol related liver diseases, haematological diseases, for example, chronic anemia and aplastic anemia; degenerative diseases of the musculoskeletal system, for example, osteoporosis and arthritis, aspirin-senstive rhinosinusitis, cystic fibrosis, multiple sclerosis, kidney diseases and cancer pain.
It has also been discovered that some cyclin-dependent kinase inhibitors can be used in combination with other anticancer agents. For example, the cytotoxic activity of cyclin-dependent kinase inhibitor flavopiridol, has been used with other anticancer agents in combination therapy.
Thus, in the pharmaceutical compositions, uses or methods of this invention for treating a disease or condition comprising abnormal cell growth, the disease or condition comprising abnormal cell growth in one embodiment is a cancer.
Particular subsets of cancers include breast cancer, ovarian cancer, colon cancer, prostate cancer, oesophageal cancer, squamous cancer and non-small cell lung carcinomas.
Methods for the Preparation of Compounds of the Formula (I)
Compounds ofthe formula (I) and the various sub-groups thereof as hereinbefore defined can be prepared by reacting an amine ofthe formula H^-A-B-R1 with an indazole 3 -carboxylic acid ofthe formula (XNII):
Figure imgf000048_0001
wherein R to R are as hereinbefore defined. The coupling reaction between the amine and the carboxylic acid (XNII) can be carried out by forming an activated derivative ofthe acid such as an acid chloride (e.g. by reaction with thionyl chloride), and then reacting the acid chloride with the amine, for example by the method described in Zh. Obs. Khim. 31, 201 (1961), and the method described in US 3,705,175.
Alternatively, and more preferably, the coupling reaction between the carboxylic acid (XNII) and the amine can be carried out in the presence of an amide coupling reagent ofthe type commonly used to form peptide linkages. Examples of such reagents include 1,3-dicyclohexylcarbodiimide (DCC) (Sheehan et al, J. Amer. Chem Soc. 1955, 77, 1067), l-ethyl-3-(3'-dimethylaminopropyl)carbodiimide (EDCI) (Sheehan et al, J. Org. Chem., 1961, 26, 2525), uronium-based coupling agents such as O-(7-azabenzotriazol-l-yl)-NNN',N'-tetramethyluronium hexafluorophosphate (HATU) (L. A. Carpino, J. Amer. Chem. Soc, 1993, 115, 4397) and phosphonium-based coupling agents such as 1-benzo- triazolyloxytris(pyrrolidino)phosphonium hexafluorophosphate (PyBOP) (Castro et al, Tetrahedron Letters, 1990, 31, 205). A preferred coupling reagent is HATU. Carbodiimide-based coupling agents are advantageously used in combination withl -hydroxybenzotriazole (HOBt) (Konig et al, Chem. Ber. , 103, 708, 2024- 2034). Preferred coupling reagents include EDC and DCC in combination with HOBt.
The coupling reaction is typically carried out in a non-aqueous, non-protic solvent such as dichloromethane, dimethylformamide or Ν-methylpyrrolidine. The reaction can be carried out at room temperature or, where the reactants are less reactive (for example in the case of electron-poor anilines bearing electron withdrawing groups such as sulphonamide groups) at an appropriately elevated temperature. The reaction may be carried out in the presence of a non-interfering base, for example a tertiary amine such as triethylamine or NN-diisopropylethylamine.
Carboxylic acids ofthe formula (XNII) can be obtained commercially.
Alternatively, compounds ofthe formula (XNII) can be prepared from compounds ofthe formula (XNIII):
Figure imgf000050_0001
(XNIII) by a sequence of reactions involving ring-opening, diazotisation, reduction and cyclisation. Ring opening ofthe substituted isatin compound to give an ortho- aminophenyl-glyoxylic acid derivative can be achieved using an aqueous alkali such as sodium hydroxide with moderate heating, for example to a temperature of 35°C. The amine can then be converted to the diazonium salt by treatment with nitrous acid (for example generated from sodium nitrite and sulphuric acid) at a reduced temperature (e.g. approximately 5°C). The diazonium salt is reduced to form a hydrazine using a reducing agent such as tin (II) chloride and is then cyclised to the indazole by a cyclo-condensation reaction.
Isatin derivatives ofthe formula (XNIII) are available commercially or can be prepared by a variety of known methods.
For example, according to the method described by Hewawasam et al, Tetrahedron Letters, 1994, 35, 7303-7306, Ν-protected anilines can be subjected to ortho- lithiation and the lithiated intermediate reacted with diethyl oxalate to give an α- ketoester which cyclises to give an isatin upon deprotection ofthe amino group.
According to the method of Garden et al, Tetrahedron Letters, 1997, 38, 1501- 1504, substituted anilines an be reacted with trichloroacetaldehyde and hydroxylamine in the presence of acid to give an α-isonitrosoacetanilide which cyclises to give an isatin.
According to the method of Kraynack et al, Tetrahedron Letters, 1998, 39, 7679- 7682, substituted isatins can be formed by the γ-dibromination of 2-oxo-indolines and subsequent hydrolysis ofthe resulting dibromo-compounds. An alternative route to compounds ofthe formula (I) involves the reaction of a substituted phenyl acetic acid amide compound ofthe formula (XIX):
Figure imgf000051_0001
with nitrous acid or an alkyl nitrite at a reduced temperature (e.g. lower than 20°C and preferably below 0°C) in the presence of a mineral acid such as hydrochloric acid or sulphuric acid or a mixture of hydrochloric acid and acetic acid, for example as described in US 3,705,175.
Compounds ofthe formula (XIX) can be prepared ter alia by reduction ofthe corresponding ørtAø-nitrophenylacetyl compound, for example under conditions analogous to those described in Morie et al, Synth. Commun., 1997, 27, 559-566.
Compounds of the formula (I) can also be prepared from other compounds ofthe formula (I) bearing suitable substituents and suitable reactive groups. For example, compounds wherein one or more of R3 to R6 are bromine or iodine, particularly iodine, can be used as intermediates for the preparation of other compounds ofthe formula (I).
In many ofthe reactions described above, it may be necessary to protect one or more groups to prevent reaction from taking place at an undesirable location on the molecule. Examples of protecting groups, and methods of protecting and deprotecting functional groups, can be found in Protective Groups in Organic Synthesis (T. Green and P. Wuts; 3rd Edition; John Wiley and Sons, 1999).
A hydroxy group may be protected, for example, as an ether (-OR) or an ester (- OC(=O)R), for example, as: a t-butyl ether; a benzyl, benzhydryl (diphenylmethyl), or trityl (triphenylmethyl) ether; a trimethylsilyl or t-butyldimethylsilyl ether; or an acetyl ester (-OC(=O)CH3, -OAc). An aldehyde or ketone group may be protected, for example, as an acetal (R-CH(OR)2) or ketal (R2C(OR)2), respectively, in which the carbonyl group (>C=O) is converted to a diether (>C(OR)2), by reaction with, for example, a primary alcohol. The aldehyde or ketone group is readily regenerated by hydrolysis using a large excess of water in the presence of acid. An amine group may be protected, for example, as an amide (-NRCO-R) or a urethane (-NRCO-OR), for example, as: a methyl amide (-NHCO-CH3); a benzyloxy amide (-NHCO-OCH2 H5, -NH-Cbz); as a t-butoxy amide (-NHCO-OC(CH3)3, -NH-Boc); a 2-biphenyl-2-propoxy amide (-NHCO-OC(CH3)2C6H4C6H5, -NH- Bpoc), as a 9-fluorenylmethoxy amide (-NH-Fmoc), as a 6-nitroveratryloxy amide (-NH-Nvoc), as a 2-trimethylsilylethyloxy amide (-NH-Teoc), as a 2,2,2- trichloroethyloxy amide (-NH-Troc), as an allyloxy amide (-NH-Alloc), or as a 2(- phenylsulphonyl)ethyloxy amide (-NH-Psec). Other protecting groups for amines, such as cyclic amines and heterocyclic N-H groups, include toluenesulphonyl (tosyl) and methanesulphonyl (mesyl) groups and benzyl groups such as apara- methoxybenzyl (PMB) group. A carboxylic acid group may be protected as an ester for example, as: an C1-7 alkyl ester (e.g., a methyl ester; a t-butyl ester); a C1.7 haloalkyl ester (e.g., a C^trihaloalkyl ester); a triC^alkylsilyl-C^alkyl ester; or a C5.2o aryl-Cι-7 alkyl ester (e.g., a benzyl ester; a nitrobenzyl ester); or as an amide, for example, as a methyl amide. A thiol group may be protected, for example, as a thioether (-SR), for example, as: a benzyl thioether; an acetamidomethyl ether (-S- CH2NHC(=O)CH3).
A more detailed description ofthe processes that can be used to prepare the compounds ofthe formula (I) can be found in the specific examples set out below.
Pharmaceutical Formulations
The invention also provides compounds ofthe formula (I) as hereinbefore defined in the form of pharmaceutical compositions.
The pharmaceutical compositions can be in any form suitable for oral, parenteral, topical, intranasal, ophthalmic, otic, rectal, intra-vaginal, or transdermal administration. Where the compositions are intended for parenteral administration, they can be formulated for intravenous, intramuscular, intraperitoneal, subcutaneous administration or for direct delivery into a target organ or tissue by injection, infusion or other means of delivery.
Pharmaceutical dosage forms suitable for oral administration include tablets, capsules, caplets, pills, lozenges, syrups, solutions, powders, granules, elixirs and suspensions, sublingual tablets, wafers or patches and buccal patches. Pharmaceutical compositions contaimng compounds ofthe formula (I) can be formulated in accordance with known techniques, see for example, Remington's Pharmaceutical Sciences, Mack Publishing Company, Easton, PA, USA.
Thus, tablet compositions can contain a unit dosage of active compound together with an inert diluent or carrier such as a sugar or sugar alcohol, eg; lactose, sucrose, sorbitol or mannitol; and/or a non-sugar derived diluent such as sodium carbonate, calcium phosphate, calcium carbonate, or a cellulose or derivative thereof such as methyl cellulose, ethyl cellulose, hydroxypropyl methyl cellulose, and starches such as corn starch. Tablets may also contain such standard ingredients as binding and granulating agents such as polyvinylpyrrolidone, disintegrants (e.g. swellable crosslinked polymers such as crosslinked carboxymethylcellulose), lubricating agents (e.g. stearates), preservatives (e.g. parabens), antioxidants (e.g. BHT), buffering agents (for example phosphate or citrate buffers), and effervescent agents such as citrate/bicarbonate mixtures. Such excipients are well known and do not need to be discussed in detail here.
Capsule formulations may be ofthe hard gelatin or soft gelatin variety and can contain the active component in solid, semi-solid, or liquid form. Gelatin capsules can be formed from animal gelatin or synthetic or plant derived equivalents thereof.
The solid dosage forms (eg; tablets, capsules etc.) can be coated or un-coated, but typically have a coating, for example a protective film coating (e.g. a wax or varnish) or a release controlling coating. The coating (e.g. a Eudragit ™ type polymer) can be designed to release the active component at a desired location within the gastro-intestinal tract. Thus, the coating can be selected so as to degrade under certain pH conditions within the gastrointestinal tract, thereby selectively release the compound in the stomach or in the ileum or duodenum.
Instead of, or in addition to, a coating, the drug can be presented in a solid matrix comprising a release controlling agent, for example a release delaying agent which may be adapted to selectively release the compound under conditions of varying acidity or alkalinity in the gastrointestinal tract. Alternatively, the matrix material or release retarding coating can take the form of an erodible polymer (e.g. a maleic anhydride polymer) which is substantially continuously eroded as the dosage form passes through the gastrointestinal tract.
Compositions for topical use include ointments, creams, sprays, patches, gels, liquid drops and inserts (for example intraocular inserts). Such compositions can be formulated in accordance with known methods.
Compositions for parenteral administration are typically presented as sterile aqueous or oily solutions or fine suspensions, or may be provided in finely divided sterile powder form for making up extemporaneously with sterile water for injection.
Examples of formulations for rectal or intra- vaginal administration include pessaries and suppositories which may be, for example, formed from a shaped moldable or waxy material containing the active compound.
Compositions for administration by inhalation may take the form of inhalable powder compositions or liquid or powder sprays, and can be administrated in standard form using powder inhaler devices or aerosol dispensing devices. Such devices are well known. For administration by inhalation, the powdered formulations typically comprise the active compound together with an inert solid powdered diluent such as lactose.
The compounds ofthe inventions will generally be presented in unit dosage form and, as such, will typically contain sufficient compound to provide a desired level of biological activity. For example, a formulation intended for oral administration may contain from 0.1 milligrams to 2 grams of active ingredient, more usually from 10 milligrams to 1 gram, for example, 50 milligrams to 500 milligrams.
The active compound will be administered to a patient in need thereof (for example a human or animal patient) in an amount sufficient to achieve the desired therapeutic effect.
Methods of Treatment
It is envisaged that the compounds ofthe formula (I) will useful in the prophylaxis or treatment of a range of disease states or conditions mediated by cyclin dependent kinases. Examples of such disease states and conditions are set out above.
Compounds ofthe formula (I) are generally administered to a subject in need of such administration, for example a human or animal patient, preferably a human.
The compounds will typically be administered in amounts that are therapeutically or prophylactically useful and which generally are non-toxic. However, in certain situations (for example in the case of life threatening diseases), the benefits of administering a compound ofthe formula (I) may outweigh the disadvantages of any toxic effects or side effects, in which case it may be considered desirable to administer compounds in amounts that are associated with a degree of toxicity.
A typical daily dose ofthe compound can be in the range from 100 picograms to 100 milligrams per kilogram of body weight, more typically 10 nanograms to 10 milligrams per kilogram of bodyweight although higher or lower doses may be administered where required. Ultimately, the quantity of compound administered will be commensurate with the nature ofthe disease or physiological condition being treated and will be at the discretion ofthe physician.
The compounds ofthe formula (I) can be administered as the sole therapeutic agent or they can be administered in combination therapy with one of more other compounds for treatment of a particular disease state, for example a neoplastic disease such as a cancer as hereinbefore defined. Examples of other therapeutic agents that may be administered together (whether concurrently or at different time intervals) with the compounds ofthe formula (I) include cytotoxic agents, agents that prevent cell proliferation or radiotherapy. Examples of such agents include but are not limited to topoisomerase inhibitors, alkylating agents, antimetabolites, DNA binders and microtubule inhibitors, such as cisplatin, cyclophosphamide, doxorubicin, irinotecan, fludarabine, 5FU, taxanes and mitomycin C.
Antifungal Use
In a further aspect, the invention provides the use ofthe compounds ofthe formula (I) as hereinbefore defined as antifungal agents.
The compounds ofthe formula (I) may be used in animal medicine (for example in the treatment of mammals such as humans), or in the treatment of plants (e.g. in agriculture and horticulture), or as general antifungal agents, for example as preservatives and disinfectants.
In one embodiment, the invention provides a compound ofthe formula (I) as hereinbefore defined for use in the prophylaxis or treatment of a fungal infection in a mammal such as a human.
Also provided is the use of a compound ofthe formula (I) for the manufacture of a medicament for use in the prophylaxis or treatment of a fungal infection in a mammal such as a human.
For example, compounds ofthe invention may be administered to human patients suffering from, or at risk of infection by, topical fungal infections caused by among other organisms, species of Candida, Trichophyton, Microsporum or Epidermophyton, or in mucosal infections caused by Candida albicans (e.g. thrush and vaginal candidiasis). The compounds ofthe invention can also be administered for the treatment or prophylaxis of systemic fungal infections caused by, for example, Candida albicans, Cryptococcus neoformans, Aspergillus flavus, Aspergillus fumigatus, Coccidiodies, Paracoccidioides, Histoplasma or Blastomyces. In another aspect, the invention provides an antifungal composition for agricultural (including horticultural) use, comprising a compound ofthe formula (I) together with an agriculturally acceptable diluent or carrier.
The invention further provides a method of treating an animal (including a mammal such as a human), plant or seed having a fungal infection, which comprises treating said animal, plant or seed, or the locus of said plant or seed, with an effective amount of a compound ofthe formula (I).
The invention also provides a method of treating a fungal infection in a plant or seed which comprises treating the plant or seed with an antifungally effective amount of a fungicidal composition as hereinbefore defined.
Differential screening assays may be used to select for those compounds ofthe present invention with specificity for non-human CDK enzymes. Compounds which act specifically on the CDK enzymes of eukaryotic pathogens can be used as antifungal or anti-parasitic agents. Inhibitors ofthe Candida CDK kinase, CKSI, can be used in the treatment of candidiasis. Antifungal agents can be used against infections ofthe type hereinbefore defined, or opportunistic infections that commonly occur in debilitated and immunosuppressed patients such as patients with leukemias and lymphomas, people who are receiving immunosuppressive therapy, and patients with predisposing conditions such as diabetes mellitus or AIDS, as well as for non-immunosuppressed patients.
Assays described in the art can be used to screen for agents which may be useful for inhibiting at least one fungus implicated in mycosis such as candidiasis, aspergillosis, mucormycosis, blastomycosis, geotrichosis, cryptococcosis, chromoblastomycosis, coccidiodomycosis, conidiosporosis, histoplasmosis, maduromycosis, rhinosporidosis, nocaidiosis, para-actinomycosis, penicilliosis, monoliasis, or sporotrichosis. The differential screening assays can be used to identify anti-fungal agents which may have therapeutic value in the treatment of aspergillosis by making use ofthe CDK genes cloned from yeast such as Aspergillus fumigatus, Aspergillus flavus, Aspergillus niger, Aspergillus nidulans, or Aspergillus terreus, or where the mycotic infection is mucon-nycosis, the CDK assay can be derived from yeast such as Rhizopus arrhizus, Rhizopus oryzae, Absidia corymbifera, Absidia ramosa, or Mucorpusillus. Sources of other CDK enzymes include the pathogen Pneumocystis carinii.
By way of example, in vitro evaluation ofthe antifungal activity ofthe compounds can be performed by determining the minimum inhibitory concentration (M.I.C.) which is the concentration ofthe test compounds, in a suitable medium, at which growth ofthe particular microorganism fails to occur. In practice, a series of agar plates, each having the test compound incorporated at a particular concentration is inoculated with a standard culture of, for example, Candida albicans and each plate is then incubated for an appropriate period at 37 °C. The plates are then examined for the presence or absence of growth ofthe fungus and the appropriate M.I.C. value is noted
The in vivo evaluation ofthe compounds can be carried out at a series of dose levels by intraperitoneal or intravenous injection or by oral administration, to mice that have been inoculated with a fungus, e.g., a strain of Candida albicans or Aspergillus flavus. The activity ofthe compounds can be assessed on the basis ofthe survival of a treated group of mice after the death of an untreated group of mice. The activity may be measured in terms ofthe dose level at which the compound provides 50% protection against the lethal effect ofthe infection (PD50).
For human antifungal use, the compounds ofthe formula (I) can be administered alone or in admixture with a pharmaceutical carrier selected in accordance with the intended route of administration and standard pharmaceutical practice. Thus, for example, they may be administered orally, parenterally, intravenously, intramuscularly or subcutaneously by means ofthe formulations described above in the section headed "Pharmaceutical Formulations".
For oral and parenteral administration to human patients, the daily dosage level of the antifungal compounds ofthe formula (I) be from 0.01 to 10 mg/kg (in divided doses), depending on inter alia the potency ofthe compounds when administered by either the oral or parenteral route. Tablets or capsules ofthe compounds may contain, for example, from 5 mg. to 0.5 g of active compound for administration singly or two or more at a time as appropriate. The physician in any event will determine the actual dosage (effective amount) which will be most suitable for an individual patient and it will vary with the age, weight and response ofthe particular patient.
Alternatively, the antifungal compounds of formula (I) can be administered in the form of a suppository or pessary, or they may be applied topically in the form of a lotion, solution, cream, ointment or dusting powder. For example, they can be incorporated into a cream consisting of an aqueous emulsion of polyethylene glycols or liquid paraffin; or they can be incorporated, at a concentration between 1 and 10%, into an ointment consisting of a white wax or white soft paraffin base together with such stabilizers and preservatives as may be required.
In addition to the therapeutic uses described above, anti-fungal agents developed with such differential screening assays can be used, for example, as preservatives in foodstuff, feed supplement for promoting weight gain in livestock, or in disinfectant formulations for treatment of non-living matter, e.g., for decontaminating hospital equipment and rooms. In similar fashion, side by side comparison of inhibition of a mammalian CDK and an insect CDK, such as the Drosophilia CDK5 gene (Hellmich et al. (1994) FEBS Lett 356:317-21), will permit selection amongst the compounds herein of inhibitors which discriminate between the human/mammalian and insect enzymes. Accordingly, the present invention expressly contemplates the use and formulations ofthe compounds ofthe invention in insecticides, such as for use in management of insects like the fruit fly.
In yet another embodiment, certain ofthe subject CDK inhibitors can be selected on the basis of inhibitory specificity for plant CDK's relative to the mammalian enzyme. For example, a plant CDK can be disposed in a differential screen with one or more ofthe human enzymes to select those compounds of greatest selectivity for inhibiting the plant enzyme. Thus, the present invention specifically contemplates formulations ofthe subject CDK inhibitors for agricultural applications, such as in the form of a defoliant or the like.
For agricultural and horticultural purposes the compounds ofthe invention may be used in the form of a composition formulated as appropriate to the particular use and intended purpose. Thus the compounds may be applied in the form of dusting powders, or granules, seed dressings, aqueous solutions, dispersions or emulsions, dips, sprays, aerosols or smokes. Compositions may also be supplied in the form of dispersible powders, granules or grains, or concentrates for dilution prior to use. Such compositions may contain such conventional carriers, diluents or adjuvants as are known and acceptable in agriculture and horticulture and they are manufactured in accordance with conventional procedures. The compositions may also incorporate other active ingredients, for example, compounds having herbicidal or insecticidal activity or a further fungicide. The compounds and compositions can be applied in a number of ways, for example they can be applied directly to the plant foliage, stems, branches, seeds or roots or to the soil or other growing medium, and they may be used not only to eradicate disease, but also prophylactically to protect the plants or seeds from attack. By way of example, the compositions may contain from 0.01 to 1 wt.% ofthe active ingredient. For field use, likely application rates ofthe active ingredient may be from 50 to 5000 g/hectare.
The invention also contemplates the use ofthe compounds ofthe formula (I) in the control of wood decaying fungi and in the treatment of soil where plants grow, paddy fields for seedlings, or water for perfusion. Also contemplated by the invention is the use ofthe compounds ofthe formula (I) to protect stored grain and other non-plant loci from fungal infestation.
EXAMPLES
The invention will now be illustrated, but not limited, by reference to the specific embodiments described in the following examples. In the examples, the compounds prepared were characterised by liquid chromatography and mass spectroscopy using two systems, the details of which are set out below. Where chlorine is present, the mass quoted for the compound is for 35C1 unless otherwise indicated. The two systems were equipped with identical chromatography columns and were set up to run under the same operating conditions. The operating conditions used are also described below.
1. Platform system
System: Waters 2790/Platform LC
Mass Spec Detector: Micromass Platform LC PDA Detector: Waters 996 PDA
Analytical conditions:
Eluent A: H2O (1% Formic Acid) Eluent B : CH3CN (1 % Formic Acid) Gradient: 5-95% eluent B Flow: 1.5 ml/min
Column: Synergi 4μm Max-RP C12, 80A, 50 x 4.6 mm (Phenomenex)
MS conditions: Capillary voltage: 3.5 kV
Cone voltage: 30 V
Source Temperature: 120 °C
2. FractionLvnx system System: Waters FractionLynx (dual analytical/prep)
Mass Spec Detector: Waters-Micromass ZQ PDA Detector: Waters 2996 PDA
Analytical conditions: Eluent A: H2O (1% Formic Acid) Eluent B: CH3CN (1% Formic Acid) Gradient: 5-95% eluent B
Flow: 1.5 ml/min
Column: Synergi 4μm Max-RP C12, 80A, 50 x 4.6 mm (Phenomenex)
MS conditions:
Capillary voltage: 3.5 kN
Cone voltage: 30 N
Source Temperature: 120 °C
Desolvation Temperature: 230 °C
The starting materials for each ofthe Examples are commercially available unless otherwise specified.
EXAMPLE 1 General Amide Preparative Procedure A
To a solution of indazole-3 -carboxylic acid (Fluka) (405 mg, 2.5 mmol, 1.0 equiv) in dichloromethane (10 ml) was added an amine or appropriately substituted aniline (3.0 mmol, 1.2 equiv), NN-diisopropylethylamine (1.6 ml, 9.0 mmol, 3.6 equiv) and O-(7-azabenzotriazol-l-yl)-NNN')N'-tetramethyluronium hexafluorophosphate (1.05 g, 2.75 mmol, 1.1 equiv). The mixture was stirred for a period of 24-72 hours and additional O-(7-azabenzotriazol-l-yl)-NNN',N'- tetramethyluronium hexafluorophosphate was added if necessary. The reaction was quenched with water (10 ml) and dichloromethane (10 ml). The compounds were purified as described in the examples below, and characterised by liquid chromatography and mass spectrometry using either ofthe systems described above.
EXAMPLE 2
General Amide Preparative Procedure B
To a suspension of 5-iodoisatin (Lancaster Synthesis) (2.2 g, 8.0 mmol, 1.0 equiv) or 5-chloroisatin (Lancaster Synthesis) (1.0 equiv.) in water (20 ml) was added ΝaOH (0.34 g, 8.48 mmol, 1.06 equiv) and the mixture was warmed to approximately 35 °C for 30 minutes to form a solution. The solution was cooled to 5 °C and a solution of sodium nitrite (0.62 g, 8.98 mmol, 1.12 equiv) was added dropwise over approximately 30 minutes, keeping the temperature below 10 °C. The whole mixture was added dropwise via a cannula to a vigorously stirred solution of concentrated sulphuric acid (1.53 g, 15.6 mmol, 1.95 equiv) in water (20 ml) keeping the temperature below 10 °C. The mixture was stirred for 20 minutes and a solution of tin (II) chloride (3.7 g, 19.52 mmol, 2.44 equiv) in concentrated hydrochloric acid (8 ml) was added dropwise. The mixture was stirred at 5 °C for 2 hours and the resulting crude 5-iodo or 5-chloro indazole-3 -carboxylic acid (a yellow solid) was isolated by filtration and washed several times with water. The yellow solid was then azeotroped with toluene (3 x 100 ml) to remove water prior to the next step. The crude product was dissolved in dichloromethane (36 ml) and split into four 8 ml portions. To the separate solutions of crude 5-iodo or 5-chloro indazole-3 -carboxylic acid in dichloromethane (8 ml) was added the appropriate amine/aniline (2.4 mmol, 1.2 equiv), NN-diisopropylethylamine (1.2 ml, 7.2 mmol, 3.6 equiv) and O-(7-azabenzotriazol-l-yl)-NNN',N'-tetramethyluronium hexafluorophosphate (0.84 g, 2.20 mmol, 1.1 equiv). The mixture was stirred for a period of 24-72 hours and was then quenched with water (8 ml) and dichloromethane (8 ml). The compounds were purified as described in the examples below, and characterised by liquid chromatography and mass spectrometry using either ofthe systems described above.
By following either preparative Procedure A or Procedure B, compounds ofthe formula (I) were prepared as described in Examples 3 to 14.
EXAMPLE 3 Ν-[4-(Methylsulphonylaminomethyl)phenyl1 - 1 H-indazole-3-carboxamide
Figure imgf000064_0001
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 119 mg (14%); LCMS 2.92 min, m/z [M+H]+ 345.
EXAMPLE 4
Preparation of N- [3 -( 1 H-tetrazol-5 -yHphenyll - 1 H-indazole-3 -carboxamide
Figure imgf000064_0002
Procedure A was followed. The water and dichloromethane layers were separated and the aqueous layer was acidified with 2N HCl to form a precipitate. The precipitate was filtered. The title compound was dried in vacuo to afford 119 mg (14%); LCMS 2.95 min, m/z [M+H]+ 306.
EXAMPLE 5 Preparation of N- 4-(acetylaminomethyl)phenyl]-l H-indazole-3 -carboxamide
Figure imgf000065_0001
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 190 mg (25%); LCMS 2.68 min, m/z [M+H]+ 309.
EXAMPLE 6
Preparation of acid N-[4-(2-oxopyrrolidin-l-yl)phenyl]-lH-indazole-3-carboxamide
Figure imgf000065_0002
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 311 mg (39%); LCMS 3.00 min, m/z [M+H]+ 321.
EXAMPLE 7
Preparation of N-[3-(oxazol-5-yl)phenv -lH-indazole-3-carboxamide
Figure imgf000066_0001
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 276 mg (36%); LCMS 3.42 min, m/z [M+H]+ 305.
EXAMPLE 8
Preparation of N- [4-( 1 H-imidazol-4-yl phenyl] - 1 H-indazole-3 -carboxamide
Figure imgf000066_0002
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was further purified by preparative HPLC to afford 1 mg (1%); LCMS 1.99 min, m/z [M+H]+ 304.
EXAMPLE 9 Preparation of N-f3-methanesulr)honylphenyl]-lH-indazole-3-carboxamide
Figure imgf000067_0001
Procedure A was followed. The layers were separated and the aqueous layer was extracted twice with dichloromethane. The combined organic layers were washed with brine, dried (MgSO ) and concentrated under reduced pressure. The title compound was purified by chromatography (SiO2), eluting with 50% ethyl acetate - petrol, to afford 114 mg (14%); LCMS 3.09 min, m/z [M+H]+ 316.
EXAMPLE 10
Preparation of N- 4-(morpholine-4-sulphonyl)phenvn - 1 H-indazole-3 -carboxamide
Figure imgf000067_0002
Procedure A was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was further purified by preparative HPLC to afford 18 mg (2%); LCMS 3.39 min,
Figure imgf000067_0003
EXAMPLE 11
Preparation of N-phenyl-5 -iodo- 1 H-indazole-3 -carboxamide
Figure imgf000068_0001
Procedure B was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 53 mg (7%); LCMS 4.11 min, m/z [M+H]+ 364.
EXAMPLE 12
Preparation of N-^-aminosulphonylphenylVS-iodo-lH-indazole-S-carboxamide
Figure imgf000068_0002
Procedure B was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 16 mg (2%); LCMS 3.30 min, m/z [M+H]+ 443.
EXAMPLE 13
Preparation of N-r4-(methylaminosulphonylmethylN)phenyl 1-5-iodo-lH-indazole-3- carboxamide
Figure imgf000069_0001
Procedure B was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 21 mg (2%); LCMS 3.48 min, m/z [M+H]+ 471.
EXAMPLE 14
Preparation of N-f 3 -methanesulphonylphenyl)-5 -iodo-1 H-indazole-3 -carboxamide
Procedure B was followed. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was further purified by preparative HPLC to afford 2 mg (1%); LCMS 4.02 min, m/z [M+H]+ 442.
EXAMPLE 15
Preparation of N- r4-(acetylammomethyr)phenvι"| -5 -iodo- 1 H-indazole-3 - carboxamide
15 A. Preparation of N-(4-amino-benzylVacetamide
To 4-aminobenzylamine (3.4 ml, 30.0 mmol, 1.0 equiv) was added pyridine (30 ml) and acetic anhydride (3.1 ml, 33.0 mmol, 1.1 equiv). The mixture was stirred at room temperature for 3 days. The reaction mixture was quenched with water and the aqueous phase was extracted with EtOAc (2 x). The combined organic layers were washed with brine, dried (MgSO ) and concentrated under reduced pressure. The title compound was purified by Biotage (SiO2, 100 g) eluting with 100% EtOAc to afford 1.47 g (30%) of the title compound.
15B. N-[4-racetylaminomethvπphenyl1-5-iodo-lH-indazole-3-carboxamide
Figure imgf000070_0001
Procedure B was followed using the amine produced in 15 A. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 16 mg (2%); LCMS 3.44 min, m/z [M+H]+ 435.
EXAMPLE 16
Preparation of N-(5 -nitro-pyridin-2-yl>5 -Iodo- 1 H-indazole-3 -carboxamide
Figure imgf000070_0002
Procedure B was followed using the amide produced in Example 16A. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was dried in vacuo to afford 5 mg (1%); LCMS 4.50 min, m/z [M+H]+ 410.
EXAMPLE 17
Preparation of 5-Morpholin-4-yl-lH-indazole-3-carboxylic acid phenylamide 17A. Preparation of 5 -Nitro- 1 H-indazole-3 -carboxylic acid
Figure imgf000071_0001
To a suspension of indazole-3-carboxylic acid (Fluka) (5 g, 31mmol) in concentrated H2SO4 (30 ml) at 0 °C was added KNO3 (3.13 g, 31 mmol). The reaction was allowed to stir overnight at room temperature, then diluted with water and the products extracted with ethyl acetate. The combined organic layers were washed with brine and then dried over MgSO4. Evaporation to dryness left the product as a yellow solid as a 7:3 mixture with the 7-nitro isomer; LCMS 2.58 min,
Figure imgf000071_0002
17B. Preparation of 5-Nitro-lH-indazole-3-carboxylic acid methyl ester
Figure imgf000071_0003
To a suspension ofthe carboxylic acid 1A (2.5 g, 12.1 mmol) in methanol (40 ml) was added concentrated hydrochloric acid (3 drops). The reaction was heated to reflux overnight. The reaction was allowed to cool to room temperature. The solid was filtered and dried in a vacuum oven to leave a yellow solid; LCMS 3.30 min, m/z [M+H]+ 222 and m/z [2M+H]+ 443.
17C. Preparation of 5-Amino-lH-indazole-3-carboxylic acid methyl ester
Figure imgf000072_0001
To a suspension ofthe nitro-indazole IB (1.23 g, 5.57 mmol) in ethanol (10 ml) was added ethyl acetate (50 ml) and then Pd/C (56 mg) under a nitrogen atmosphere. The atmosphere was exchanged for H2, and H2 was bubbled through the reaction mixture for 5 minutes. After three hours the compound was observed to have dissolved completely. The reaction mixture was filtered though Celite and the filtrate evaporated to dryness to leave the product amine [which contains approximately 25% ofthe 7-nitro isomer] as a yellow solid; LCMS 2.68 min, [M+H] + 192.
17D. Preparation of 5-morpholin-4-yl-lH-indazole-3-carboχylic acid methyl ester
Figure imgf000072_0002
To a mixture of 5-amino-lH-indazole-3-carboxylic acid methyl ester and 7-amino- 1 H-indazole-3 -carboxylic acid methyl ester (as synthesized above) (1.91 g, 10.0 mmol, 1.0 equiv) in DMF (20 ml) was added NN-diisopropylethylamine (5.2 ml, 30.0 mmo, 3.0 equiv), tetrabutylammonium iodide (739 mg, 2.0 mmol, 0.2 equiv) and bis(chloroethyl)ether (1.4 ml, 12.0 mmol, 1.2 equiv). The solution was heated to 90°C for 15 h. The DMF was carefully removed under reduced pressure in a fume hood. The resultant mixture was partitioned between ethyl acetate and water. The organic layer was washed with brine, dried (MgSO ), and concentrated under reduced pressure. The compound was purified by column chromatography to afford 5-morpholin-4-yl-lH-indazole-3-carboxylic acid methyl ester 300 mg (11%); LCMS 2.28 min, m/z [M+H]+ 262.
17E. Preparation of 5-Morpholin-4-yl-lH-indazole-3-carboxylic acid phenylamide
Figure imgf000073_0001
To 5 -morpholin-4-yl-l H-indazole-3 -carboxylic acid methyl ester (91 mg, 0.35 mmol, 1.0 equiv) in THF (3 ml) was added potassium hydroxide (116 mg, 1.75 mmol, 5.0 equiv) in water (3.5 ml). The mixture was heated to reflux for 3.5 h. The mixture was evaporated to dryness and 2N hydrochloric acid was added. The resultant precipitate was collected and azeotroped with toluene (3 x 10 ml).
The crude 5-Morpholin-4-yl-l H-indazole-3 -carboxylic acid solid LCMS 1.78 min, m/z [M+H]+ 248 was used directly in Procedure A. The aqueous was extracted with dichloromethane. The combined organic layers were washed with brine, dried (MgSO4) and were removed under reduced pressure. The title compound was further purified by preparative HPLC to afford 9 mg (16%); LCMS 3.11 min, m/z [M+H 323.
EXAMPLE 18
Preparation of 5-Nitro-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenylVamide
Figure imgf000073_0002
Procedure B was followed using 5-Nitro-lH-indazole-3-carboxylic acid (Example
17A) and 4-amino-benzenesulphonamide. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane.
The title compound was further purified by preparative HPLC as a 8:2 mixture with the 7-nitro isomer; LCMS 2.89 min, m/z [M+H]+ 362.
EXAMPLE 19 Preparation of 5-Nitro-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl; phenvD-amide
Figure imgf000074_0001
Procedure B was followed using 5-Nitro-lH-indazole-3-carboxylic acid (Example 17A) and (4-amino-phenyl)-N-methyl-methane sulphonamide. Water and dichloromethane were removed by filtration and the solid was triturated with water and dichloromethane. The title compound was further purified by preparative HPLC: LCMS 3.30 min, m/z [M+H]+ 390.
EXAMPLE 20 General Palladium (0) Cross-CouplinR Procedure C
To 5-iodo-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide (Example 13) (47 mg, 0.1 mmol, 1.0 equiv.) in toluene (0.8 ml) was added the relevant palladium (0) catalyst (0.02 mmol, 0.2 equiv.). The reaction mixture was degassed by bubbling nitrogen through the mixture and was stirred at room temperature for 5 minutes. The corresponding heteroaryl boronic acid (0.3 mmol, 3.0 equiv) in ethanol (0.8 ml) was added and stirred for 5 minutes. To the mixture was added a solution of potassium carbonate (138 mg, 1.0 mmol, 10 equiv.) in water (2.0 ml) followed by methanol (2.0 ml) and the mixture was sealed in a vial under nitrogen. The mixture was heated between 120 °C and 150 °C for 15 minutes using a maximum 100-watt power in a microwave. Methanol (5 ml) was added and all solvents were removed under reduced pressure. The compounds were purified as described in the Examples below, and characterised by liquid chromatography and mass spectrometry using either ofthe systems described above.
EXAMPLE 21 Preparation of 5 -Thiophen-2-yl-l H-indazole-3 -carboxylic acid (4- methylsulphamoylmetliyl-phenylVamide
Figure imgf000075_0001
Procedure C was followed using bis(tri-t-butylphosphine)palladium (0) (Strem) and thiophene-2-boronic acid (Maybridge). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 22 mg (52%); LCMS 3.97 min, m/z [M+H]+ 427.
EXAMPLE 22 Preparation of 5-(3,5-Dimethyl-isoxazol-4-yl -lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenylVamide
Figure imgf000075_0002
Procedure C was followed using bis(tri-t-butylphosphine)palladium (0) (Strem) and 3,5-dimethylisoxazole-4-boronic acid (Maybridge). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 5 mg (11%); LCMS 3.54 min, m/z [M+H]+ 440.
EXAMPLE 23
Preparation of 5 -Furan-2-yl-l H-indazole-3 -carboxylic acid (4- methylsulphamoylmefhyl-phenvπ-amide
Figure imgf000076_0001
Procedure C was followed using bis(tri-t-butylphosphine)palladium (0) (Strem) and furan-2 -boronic acid (Lancaster). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 15 mg (37%): LCMS 3.82 min, m/z [M+H]+ 411.
EXAMPLE 24
Preparation of 5-Benzofuran-2-yl-lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenvD-amide
Figure imgf000076_0002
Procedure C was followed using tetrakis(triphenylphosphine)palladium(0) (Aldrich) and benzo[b]furan-2-boronic acid (Lancaster). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 20 mg (36%): LCMS 4.33 min, m/z [M+H]+ 461.
EXAMPLE 25
Preparation of 5 -Chloro- 1 H-indazole-3 -carboxylic acid (4- methylsulphamoylmethyl-phenvD-amide
Figure imgf000077_0001
To a solution of 5-iodo- 1 H-indazole-3 -carboxylic acid (4-methylsulphamoyl- methyl-phenyl)-amide (Example 13) (42 mg, 0.09 mmol, 1.0 equiv.) in d6- dimethyl sulphoxide (0.7 ml) was added copper(I) chloride (401 mg, 4.05 mmol, 45 equiv.). The mixture was heated to 180 °C for 15 minutes using a maximum 50- watt power in a microwave. The title compound was purified by preparative HPLC to afford 14 mg (41%); LCMS 3.54 min, m/z [M+H]+ 379.
EXAMPLE 26
Preparation of lH-Indazole-3 -carboxylic acid (4-surphamoyl-phenyiy amide
Figure imgf000077_0002
To indazole-3 -carboxylic acid (1 equiv.) inN-methyl pyrrolidinone (5 ml) was added EDC (1.2 equiv.), HOBT (1.2 equiv.), NMM (1.2 equiv.) and then 4- sulphamoyl aniline (1.3 equiv.) at room temperature. The reaction was heated to 100 °C for 24 hours. A further equivalent of EDC was added and the reaction heated at 100 °C for a further 4 hours. Water was added to the reaction and the aqueous layer extracted with ethyl acetate (2 x 30ml). The combined organic layers were dried (MgSO4) and the solvent removed under reduced pressure. The desired product was isolated by column chromatography. LCMS 2.72 min, m/z [M+H]+ 317. EXAMPLE 27
Preparation of lH-Indazole-3-carboxylic acid phenylVamide
Figure imgf000078_0001
By following the procedure described in Example 26, but using aniline instead of 4- sulphamoyl aniline, the title compound was prepared. LCMS 3.44 min, m/z [M+H]+ 238.
EXAMPLE 28
lH-Indazole-3 -carboxylic acid (6-chloro-pyridin-3-yl -amide
Figure imgf000078_0002
By following procedure A, the title compound was prepared; LCMS m/z [M+H]+ 273, 3.42 min.
EXAMPLE 29
Preparation of 5-Thiazol-2-yl-lH-indazole-3-carboχylic acid (4- methylsulphamoylmethyl-phenylVamide
Figure imgf000078_0003
A solution of 5-iodo-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide (Example 13) (47 mg, 0.1 mmol, 1.0 equiv.) in THF (1 ml) was degassed by bubbling nitrogen though the solution. Bis(tri-tert- butylphosphine)palladium(O) (23 mg, 0.02 mmol, 0.2 equiv.) was added, the solution was degassed with nitrogen and stirred for 5 minutes. 2-Thiazolylzinc bromide (2 ml of a 0.5M solution in THF, 1.0 mmol, 10 equiv) was added and the mixture was heated to 195 °C for 15 minutes using 100 watts in a CEM microwave. The reaction was quenched with methanol and evaporated to dryness. The title compound was purified by Biotage (SiO2), eluted with 80% EtO Ac-petrol, to afford 18 mg (42%); LCMS 3.27 min, m/z [M+H]+ 428.
EXAMPLES 30 - 59
By following procedures A, B or C as set out above, and using the appropriate starting materials, the compounds set out in Table 1 below were prepared.
Table 1
Figure imgf000079_0001
Figure imgf000080_0001
Figure imgf000081_0001
Figure imgf000082_0001
Figure imgf000083_0001
5-Amino-lH-indazole-3-carboxylic acid phenylamide
Figure imgf000083_0002
To a suspension ofthe nitro-indazole of Example 55 (49 mg, 0.17 mmol) in ethanol (5 ml) was added Pd/C (0.1 equiv.) under a nitrogen atmosphere. The atmosphere was exchanged for H2, and H2 was bubbled through the reaction mixture for 5 minutes. The reaction was left for 16 hours and flushed with N2, following which the reaction mixture was filtered though Celite and the filtrate evaporated to dryness to leave the product amine as a red-brown solid. LCMS 2.09 min m/z [M+H]+ 253.
EXAMPLE 61
5-Iodo-lH-indazole-3-carboxylic acid (4-methylaminosulphonylmethyl-phenylV amide
61 A. (4-methylaminosulphonylmethyl-phenvπ-amine
To aminobenzylamine (1 g, 8.18 mmol) in CH2C12 (50 ml) at 0 °C was added Et3N (2.28 ml, 16.3 mmol) followed by MesCl (0.63 ml, 8.18 ml), and the reaction was stirred at 0 °C for 1 hour. The reaction mixture was diluted with CH2C12; and washed twice with water. The combined organic layers were dried, filtered and evaporated to dryness. The product was purified by trituration with 5% MeOH- CH2C12.
61B. 5-Iodo-lH-indazole-3-carboxylic acid (4-methylaminosulphonylmethyl- phenvD-amide
Figure imgf000084_0001
The product of Example 61 A was reacted with 5-iodo indazole-3 -carboxylic acid using method B to give the title compound. LCMS 3.66 min m/z [M+H]+ 471.
EXAMPLE 62
62A. 5-Amino-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenylVamide
Figure imgf000085_0001
To a suspension ofthe nitro-indazole of Example 18 (1.0 g, 2.77 mmol) in DMF:EtOH (1:1, 20ml) was added Pd/C (0.27 mg, 0.1 eq) under a nitrogen atmosphere. The atmosphere was exchanged for H2, and H2 was bubbled through the reaction mixture for 5 minutes. After three hours the compound was observed to have dissolved completely. The reaction mixture was filtered though Celite and the filtrate evaporated to dryness to leave the product amine. Purification by preparative HPLC gave the desired product. LCMS 0.58 min m/z [M+H]+ 332.
62B. 7-Amino-l H-indazole-3 -carboxylic acid (4-sulphamoyl-phenylVamide
Figure imgf000085_0002
The 7-amino isomer was isolated as a minor product the reaction described in Example 62A. LCMS 2.32 min m/z [M+H]+ 332.
EXAMPLE 63
5- [3 -(2-Chloro-ethyl)-ureidol-l H-indazole-3 -carboxylic acid (4- methylsulphamoylmethyl-phenylVamide
63A. Preparation of 5-Nitro-lH-indazole-3-carboxylic acid
Figure imgf000085_0003
To a suspension of indazole-3 -carboxylic acid (Fluka) (5 g, 31 mmol) in concentrated H2SO4 (30 ml) at 0 °C was added KNO3 (3.13 g, 31 mmol). The reaction was allowed to stir overnight at room temperature, then diluted with water and the products were extracted with ethyl acetate. The combined organic layers were washed with brine and then dried over MgSO4. Evaporation to dryness left the product as a yellow solid as a 7:3 mixture with the 7-nitro isomer; LCMS 2.58 min, m/z [M+H]+ 208.
63B. Preparation of 5-Nitro-lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenylVamide
Figure imgf000086_0001
To the nitro-lH-indazole-3-carboxylic acid (1 equiv.) of Example 63A in DMF (0.3 M) was added EDC (1.2 equiv.), HOBT (1.2 equiv.), NMM (1.2 equiv.) and then 4- methylsulphamoylmethyl-phenylamine (1.3 equiv.) at room temperature. The reaction was heated to 70 °C for 2 hours and then stirred at room temperature for 48 hours. Water was added to the reaction mixture and the precipitated product was filtered. The solid was washed with water, then a small volume of MeOH, and then dried in a vacuum oven to leave a yellow solid.
63C. Preparation of 5-Amino-lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenylVamide
Figure imgf000086_0002
To a suspension ofthe resulting nitro-indazole (1.0 g, 2.57 mmol) in ethanol: DMF (1:1, 20 ml) was added Pd/C (0.1 equiv.) under a nitrogen atmosphere. The atmosphere was exchanged for H2, and H2 was bubbled through the reaction mixture for 5 minutes. After three hours the compound was observed to have dissolved completely. The reaction mixture was filtered though Celite and the filtrate evaporated to dryness to leave the product amine as a brown solid.
63D. 5- [3 -(2-Chloro-ethylVureidol-l H-indazole-3 -carboxy lie acid (4- methylsulphamoylmethyl-phenylVamide
Figure imgf000087_0001
To a suspension ofthe amine (0.28 mmol) in THF (1 ml) at room temperature was added 2-chloroethyl isocyanate (0.42 mmol, 1.5 eq). The reaction was heated to 70 °C for 4 hours. The colour ofthe suspension changed from light brown to a much darker brown. Water (10 ml) was added to quench the reaction and the precipitate was filtered. The solid was washed with a portion of water and the THF and dried to leave a grey product. LCMS 2.88 min m/z [M+H]+ 465/467.
EXAMPLE 64
5-Iodo-lH-indazole-3-carboxylic acid piperidin-4-ylamide
Figure imgf000087_0002
To a solution ofthe compound of Example 30 (0.16 g, 0.36 mmol) at 0 °C, in a mixture of THF: H2O (9.5 ml: 4 ml) was added LiOH (30 mg, 0.72 mmol) followed by MeOH (4 ml). The reaction was stirred at room temperature, and when no reaction occurred the total LiOH added was increased to 150 mg. The reaction mixture was heated at 60 °C for 8 hours, and then evaporated to dryness. The product was purified by preparative HPLC to afford 40 mg, m/z [M+H]+ 371.
EXAMPLE 65 5-Chloro-lH-indazole-3-carboxylic acid f4-(acetylamino-methyl)-phenyl]-amide
Figure imgf000088_0001
N-(4-Amino-benzyl)-acetamide produced by the method of Example 15A was reacted with 5-chloro- lH-indazole-3-carboxylic acid following procedure B to give the title compound. LCMS 3.90 min m/z [M+H]+ 343.
EXAMPLE 66
Preparation of lH-Indazole-3 -carboxylic acid [1 -(2,2,2 trifluoro-acetvD-Piperidin-
4- yl] -amide
66 A. lH-Indazole-3 -carboxylic acid piperidin-4-ylamide.TFA salt
To a suspension of the compound of Example 57 (0.4 g, 1.16 mmol) in DCM (30 ml) at 0 °C was added TFA (3 ml), and the reaction was stirred at room temperature for lhour. The mixture was evaporated down, and then azeotroped with toluene to dryness. The solid was triturated with ether to afford the title compound (0.3g).
66B. lH-Indazole-3 -carboxylic acid [l-(2,2,2 trifluoro-acetyl)-piperidin-4-yl]- amide
Figure imgf000088_0002
To a suspension of 1 H-indazole-3 -carboxylic acid piperidin-4-ylamide.TFA salt, (the product of 6 A) (50 mg, 0.2 mmol) in dichloromethane (0.5 ml) and pyridine (0.5 ml) at 0 °C was added dropwise methanesulphonic anhydride (0.2 mmol), and the mixture was allowed to warm up to room temperature. The reaction mixture was diluted with water and washed with ethyl acetate. The combined organic layers were dried (MgSO4), filtered and evaporated to dryness to give a yellow oil. The title compound was purified by column cliromatography, by elution with 2% MeOH/EtOAc then 5% MeOH/EtOAc, to afford 28mg ofthe title compound. LCMS 3.34 min, m/z [M+H]+ 341.
EXAMPLE 67 Preparation of lH-mdazole-3 -carboxylic acid piperidin-4-ylamide
Figure imgf000089_0001
To a suspension ofthe compound of Example 57 (0.4 g, 1.16 mmol) in CH2C12 (10 ml) at 0 °C was added TF A (3 ml), and the reaction was stirred at room temperature for lhour. The reaction mixture was evaporated to dryness and then azeotroped with toluene. The product was triturated with ether. The sample was neutralised, and then purified by preparative HPLC to afford the purified product 8mg. m/z
Figure imgf000089_0002
EXAMPLE 68 lH-Indazole-3-carboxylic acid (l-acetyl-piperidin-4-yl -amide
Figure imgf000089_0003
To a suspension ofthe compound of Example 67 (50 mg, 0.2 mmol) in CH2CI2 (0.5 ml) and pyridine (0.5 ml) at 0 °C was added acetic anhydride (0.22 mmol) dropwise, and the reaction was allowed to warm up to room temperature. The reaction mixture was diluted with water, and washed with ethyl acetate. The combined organic layers were dried, filtered and evaporated to give a yellow oil. Column chromatography using 5% MeOH/ CH2C12 then 7% MeOH/ CH2C12 afforded 20mg of product, m/z [M+H]+ 287.
EXAMPLE 69 lH-mdazole-3-carboxylic acid (l-methanesulphonyl-piperidin-4-yl)-amide
Figure imgf000090_0001
To a suspension ofthe compound of Example 67 (33 mg, 0.13 mmol ) was added Et3N (0.054 ml, 0.39 mmol) followed by THF (0.5 ml), DMSO (0.5 ml) and then methanesulphonyl chloride (0.01 ml, 0.13 mmol). The reaction was stirred at room temperature overnight. The reaction mixture was reduced by evaporation, and purified by preparative HPLC to afford lOmgs ofthe product, m/z [M+H]+ 323.
EXAMPLE 70
lH-Indazole-3 -carboxylic acid (4-fluoro-phenyl -amide
70A. N-(4-Fluoro-phenyl -2-(2-nitro-ρhenyl -acetamide
Figure imgf000090_0002
To (2-Nitro-ρhenyl)-acetic acid (1 equiv.) in DCM (0.3 M) was added EDC (2 equiv.), HOBT (2 equiv.), NMM (2 equiv.) and then corresponding amine (1.5 equiv.) at room temperature. The reaction was left at room temperature for 5 hours. The reaction was diluted with water and extracted with DCM (x3). The combined organic layers were washed with brine and dried over MgSO . The product was filtered and evaporated to dryness to leave a yellow solid, which was taken onto the next reaction; LCMS MH+ 275, RT 3.57 min.
70B . 2-C2- Amino-phenyl)-N-(4-fluoro-phenyiy acetamide
Figure imgf000091_0001
To a suspension ofthe nitro compound (7 g, 25.5 mmol) in EtOH (225 ml) was added Pd/C (0.1 eq) under a nitrogen atmosphere. The atmosphere was exchanged for H2, and H2 was bubbled through the reaction mixture for 5 minutes. After 48 hours the reaction mixture was filtered though Celite and the filtrate evaporated to dryness to leave the product amine, which was taken on to the next reaction; LCMS MH+ 245, RT 2.57 min.
70C. lH-Indazole-3 -carboxylic acid (4-fluoro-phenylVamide
Figure imgf000091_0002
To a solution ofthe amine (3.0 g, 12.2 mmol) in toluene (122 ml) was added acetic anhydride (3.9 ml, 40.5 mmol) at room temperature. The reaction was heated to 90- 95 °C. To this mixture was added isopentyl nitrate (3.4 ml, 24.6 mmol) dropwise over a period of about 20 minutes, at 90-95 °C. The mixture was left for 90 minutes, and then heated to 105 °C for 16 hours. The reaction had turned from a yellow to a red suspension. The reaction was evaporated to dryness and then taken up in EtOAc and washed with water. The organic layer was extracted with brine and dried over MgSO4. The product was filtered and evaporated to dryness in vacuo to leave an oil which was purified by HPLC; LCMS MH+ 2.56, RT 3.69 min.
EXAMPLE 71
4-Bromo-l H-indazole-3 -carboxylic acid (4-fluoro-phenylVamide 71A. Preparation of 5-nitro-lH-indazole-3-carboχylic acid (4-fluorophenylVamide
Figure imgf000092_0001
To a solution of 5-nitro-lH-indazole-3-carboxylic acid (Example 17A) (6.5 g, 31.5 mmol, 1.0 equiv) in DMF (200 ml) was added 4-fluoroaniline (33.3 ml 34.6 mmol, 1.1 equiv), HOBt (5.1 g, 37.7 mmol, 1.2 equiv) and EDC (7.2 g, 37.7 mmol, 1.2 equiv). The mixture was stirred for a period of 72 hours. The solvent was removed under reduced pressure and the resulting solid suspended in ethyl acetate and aqueous sodium hydrogen carbonate. The precipitate was collected, resuspended in aqueous sodium hydrogen carbonate and stirred for 10 mins. The solid was collected and dried in a vacuum oven to afford the title compound (7.77 g, 82%) as a 8:2 mixture with the 7-nitro isomer; LCMS 3.83 min, m/z [M+H]+ 300.
71B. Preparation of 5-amino-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide
Figure imgf000092_0002
A mixture of 5-nitro-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide (7.3 g, 24.3 mmol), 10% Pd/C (0.7 g), ethanol (200 ml) and DMF (200ml) under an atmosphere of nitrogen was stirred under an atmosphere of hydrogen for 18 hours. Then the catalyst was removed and the filtrate was evaporated to dryness, to give the title compound (4.94 g, 75%) as a 8:2 mixture with the 7-nitro isomer; LCMS 1.95 min, m/z [M+H]+ 270. 71C. Preparation of 5-Amino-4-bromo-lH-indazole-3-carboxylic acid (4- fluorophenvD-amide
Figure imgf000093_0001
Bromine was added dropwise to a stirred suspension of 5 -amino- 1 H-indazole-3 - carboxylic acid (4-fluorophenyl)-amide (4.9 g, 18.3 mmol) in MeOH (10.5 ml) at - 5 °C The reaction mixture was stirred at -5 °C for 1 hour, and then allowed to warm to 10 °C. The reaction was poured into aqueous sodium thiosulphate solution and the suspension was stirred. The solid was collected, washed with water and then dried in a vacuum oven to afford the title compound 32C (6.9 g) that was used without further purification: LCMS 2.89 min, m/z [M+H]+ 348.
71D. Preparation of 4-bromo-l H-indazole-3 -carboxylic acid (4-fluorophenylV amide
Figure imgf000093_0002
To a solution ofthe 5-amino-4-bromo-l H-indazole-3 -carboxylic acid (4- fluorophenyl)~amide (1.5 g, 4.2 mmol) in DMF (14 ml) was added the isopentyl nitrate (0.89 ml, 6.4 mmol) slowly at 65 °C. After 5 minutes, effervescence was noted. The reaction left for a further 2 hours and allowed to cool. HCl (1 M, aq.) was added to the reaction and the product was filtered off. The solid was washed with water and evaporated down from toluene (x2). The compound was purified by prep HPLC; LCMS MH+ 334/336, RT 3.65 min.
EXAMPLE 72 5-Methyl-lH-indazole-3-carboχylic acid f4-fluoro-phenyl)-amide
72 A. Preparation of 5 -methyl- 1 H-indazole-3 -carboxylic acid
Figure imgf000094_0001
To a suspension of 5-methylisatin (Lancaster Synthesis) (5.8 g, 36.0 mmol) in water (90 ml) was added NaOH (1.53 g, 38.2 mmol, 1.1 equiv) and the mixture was warmed to approximately 35 °C for 30 minutes to form a solution. The solution was cooled to 5 °C and a solution of sodium mtrite (2.78 g, 40.3 mmol, 1.1 equiv) was added dropwise over approximately 30 minutes, keeping the temperature below 10 °C. The whole mixture was added dropwise via a cannula to a vigorously stirred solution of concentrated sulphuric acid (7.3 g, 74.4 mmol, 2.0 equiv) in water (90 ml) keeping the temperature below 10 °C. The mixture was stirred for 20 minutes and a solution of tin (II) chloride (16.7 g, 74.4 mmol, 2.4 equiv) in concentrated hydrochloric acid (34 ml) was added dropwise. The mixture was stirred at 5 °C for 2 hours and the resulting crude 5-methylindazole-3-carboxylic acid was isolated by filtration and washed several times with water. The yellow solid was then azeotroped with toluene (3 x 100 ml) to remove water prior to the next step to leave a yellow/green solid. LCMS MH1" 177, RT 2.40 min.
72B. 5-Methyl-lH-indazole-3-carboxylic acid (4-fluoro-phenyl -amide
Figure imgf000094_0002
To the carboxylic acid (1 equiv.) in DCM (0.3 M) was added EDC (1.2 equiv.), HOAT (1.2 equiv.), and then corresponding amine (1.3 equiv.) at room temperature. The reaction was left at room temperature for 48 hours. The reaction was diluted with water and extracted with EtOAc (x3). The combined organic layers were washed with brine and dried over MgSO4. The product was filtered and evaporated to dryness to leave a yellow solid. The product was triturated with DCM to yield the product; MH+ 270, RT 4.08 min.
EXAMPLE 73
6-Bromo-l H-indazole-3 -carboxylic acid (4-fluoro-phenvD-amide
73 A. Preparation of 6-bromo-lH-indazole-3-carboxylic acid
Figure imgf000095_0001
To a suspension of 6-bromoisatin (Richman) (5.0 g, 22.1 mmol) in water (55 ml) was added NaOH (0.94 g, 23.5 mmol, 1.1 equiv) and the mixture was warmed to approximately 35 °C for 30 minutes to form a solution. The solution was cooled to 5 °C and a solution of sodium nitrite (1.70 g, 24.8 mmol, 1.1 equiv) was added dropwise over approximately 30 minutes, keeping the temperature below 10 °C. The whole mixture was added dropwise via a cannula to a vigorously stirred solution of concentrated sulphuric acid (4.48 g, 45.7 mmol, 2.0 equiv) in water (55 ml) keeping the temperature below 10 °C. The mixture was stirred for 20 minutes and a solution of tin (II) chloride (10.2 g, 54.0 mmol, 2.4 equiv) in concentrated hydrochloric acid (21 ml) was added dropwise. The mixture was stirred at 5 °C for 2 hours and the resulting crude 5-methylindazole-3-carboxylic acid was isolated by filtration and washed several times with water. The yellow solid was then azeotroped with toluene (3 x 100 ml) to remove water prior to the next step to leave a yellow/green solid. LCMS MH+ 238/240 (79Br/81Br), RT 2.69 min. 73B. Preparation of 6-Bromo-lH-indazole-3-carboxylic acid (4-fluoro-phenylV amide
Figure imgf000096_0001
To 6-bromo-l H-indazole-3 -carboxylic acid (1 equiv.) in DCM (0.3 M) was added EDC (1.2 equiv.), HO AT (1.2 equiv.), and then corresponding amine (1.3 equiv.) at room temperature. The reaction was left at RT for 4 hours. The reaction was diluted with water and extracted with EtOAc (x2). The combined organic layers were washed with brine and dried over MgSO4. The product was filtered and evaporated to dryness to leave a yellow solid. The product was triturated with DCM, and purified further by prep HPLC; MH+ 334/336 (79Br/81Br), RT 4.32 min.
EXAMPLES 74 - 80
By following the procedures described in the examples above, and using the appropriate starting materials, the compounds set out in Table 2 below were prepared.
Table 2
Figure imgf000096_0002
Figure imgf000097_0001
EXAMPLE 81
Preparation of 3 - l"(5-Chloro- 1 H-indazole-3 -carbonylV amino] -pyrrolidine- 1 - carboxylic acid methyl ester
Figure imgf000098_0001
To a solution of 5-chloro-lH-indazole-3-carboxylic acid (l-benzyl-pyrrolidin-3-yl)- amide (Example 37) (639 mg, 1.8 mmol, 1 equiv) in dichloromethane (9 ml) was added 1-chloroethyl chloroformate (0.39 ml, 3.6 mmol, 2.0 equiv) at 0 °C. The mixture was heated to reflux for 1 hour, cooled and evaporated under reduced pressure. The resultant oil was dissolved in methanol and heated at reflux for 15 hours. The solvents were removed under reduced pressure and the crude mixture was purified by preparative HPLC to afford the title compound 15 mg (3%); LCMS 2.29 min, m/z [M35C1)+H]+ 323.
EXAMPLE 82
Preparation of 5 -Morpholin-4-yl-l H-indazole-3 -carboxylic acid (6-chloro-pyridin- 3-ylVamide
Figure imgf000098_0002
To 5-morpholin-4-yl-lH-indazole-3-carboxylic acid methyl ester (91 mg, 0.35 mmol, 1.0 equiv) (Example 17D) in THF (3 ml) was added potassium hydroxide (116 mg, 1.75 mmol, 5.0 equiv) in water (3.5 ml). The mixture was heated to reflux for 3.5 hours. The mixture was evaporated to dryness and 2N hydrochloric acid was added. The resultant precipitate was collected and azeotroped with toluene (3 x 10 ml).
The crude 5 -Morpholin-4-yl-l H-indazole-3 -carboxylic acid solid LCMS 1.78 min, m/z [M+H]+ 248 was used directly in Procedure A. The aqueous was extracted with dichloromethane. The combined organic layers were washed with brine, dried (MgSO4) and were removed under reduced pressure. The title compound was further purified by preparative HPLC to afford 8 mg (13%); LCMS 3.02 min, m/z [M(35C1)+H]+ 358.
EXAMPLE 83
5 -Chloro- 1 H-indazole-3 -carboxylic acid f5-(tetrahvdro-furan-2-ylV [T ,3,4]thiadiazol-2-yl]-amide
Figure imgf000099_0001
Following procedure B gave the title compound; m/z [M+H]+ 350.
EXAMPLE 84
Preparation of 5-pyrrolidin-l-yl-lH-indazole-3-carboxylic acid phenylamide
Figure imgf000099_0002
To 5-Amino-lH-indazole-3-carboxylic acid phenylamide (83 mg, 0.43 mmol, 1.0 equiv) in DMF (1.7 ml) was added NN-diisopropylethylamine (0.23 ml, 1.30 mmol, 3.0 equiv), tetrabutylammonium iodide (32 mg, 0.09 mmol, 0.2 equiv) and 1,4-dibromobutane (0.062 ml, 0.52 mmol, 1.2 equiv). The solution was heated to 90 °C for 15 hours. The mixture was concentrated under reduced pressure and purified by preparative HPLC to afford the title compound 18 mg (14%), LCMS 3.36 min, m/z [M+H]+ 307. EXAMPLE 85
Preparation of 5-Biphenyl-2-yl-lH-indazole-3-carboxylic acid phenylamide
Figure imgf000100_0001
Procedure C was followed using bis(tri-t-butylphosphine)palladium (0) (Strem) and 2-biphenylboronic acid (Lancaster). The solid was triturated with water. The title compound was further purified by preparative HPLC to afford 5 mg (13%): LCMS 5.12 min, m/z [M+H\+ 390.
EXAMPLE 86
Preparation of 5 -( 1.1 -Dioxo- 1 lambda* 6 * -isothiazolidin-2-ylV 1 H-indazole-3 - carboxylic acid phenylamide
Figure imgf000100_0002
To 5-Amino-lH-indazole-3-carboxylic acid phenylamide (83 mg, 0.43 mmol, 1.0 equiv) in DMF (1.7 ml) was added NN-diisopropylethylamine (0.23 ml, 1.30 mmol, 3.0 equiv), tetrabutylammonium iodide (32 mg, 0.09 mmol, 0.2 equiv) and 3-chloropropanesulphonyl chloride (0.092 ml, 0.52 mmol, 1.2 equiv). The solution was heated to 90 °C for 15 hours. The mixture was concentrated under reduced pressure and purified by preparative HPLC to afford the title compound 9 mg (6%), LCMS 3.32 min, m/z [M+H]+ 357.
EXAMPLE 87
Preparation of 5 -Phenethyl-1 H-indazole-3 -carboxylic acid phenylamide
Figure imgf000101_0001
To 5-Iodo-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide (50 mg, 0.13 mmol, 1.0 equiv) in THF (1.3 ml) was added bis(triphenylphosphine)palladium(II) chloride (2 mg), Copper(I) iodide (1 mg), 2N NaOMe in MeOH (0.33 ml) and fluorophenylacetylene (30 mg, 0.16 mmol, 1.2 equiv). The mixture was stirred for 15 hours and concentrated under reduced pressure. 5-(6-Fluoro-3-vinyl-hepta-3,5- dien-l-ynyl)-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide was purified by preparative HPLC, m/z 374, 4.81 min. To 5-(6-Fluoro-3-vinyl-hepta-3,5-dien-l- ynyl)-l H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide in ethanol (13 ml) was added 10% palladium on carbon (13 mg). A hydrogen atmostphere was added and the mixture was stirred overnight. The mixture was filtered through Celite™ and concentrated under reduced pressure. The title compound was purified by preparative HPLC to afford 5 mg, m/z 342, 4.86 min.
BIOLOGICAL ACTIVITY
EXAMPLE 88 Measurement of CDK2 Kinase Inhibitory Activity (ICsp
Compounds ofthe invention were tested for kinase inhibitory activity using the following protocol. 1.7 μl of active CDK2/CyclinA (Upstate Biotechnology, lOU/μl) is diluted in assay buffer (250μl of 10X strength assay buffer (200mM MOPS pH 7.2, 250mM β- glycerophosphate, 50mM EDTA, 150mM MgCl2), 11.27 μl lOmM ATP, 2.5 μl IM DTT, 25 μl lOOmM sodium orthovanadate, 708.53 μl H2O), and 10 μl mixed with 10 μl of histone substrate mix (60 μl bovine histone HI (Upstate
Biotechnology, 5 mg/ml), 940 μl H2O, 35 μCi γ33P-ATP) and added to 96 well plates along with 5 μl of various dilutions ofthe test compound in DMSO (up to 2.5%). The reaction is allowed to proceed for 5 hours before being stopped with an excess of ortho-phosphoric acid (30 μl at 2%).
γ33P-ATP which remains unincorporated into the histone HI is separated from phosphorylated histone HI on a Millipore MAPH filter plate. The wells ofthe MAPH plate are wetted with 0.5% orthophosphoric acid, and then the results ofthe reaction are filtered with a Millipore vacuum filtration unit through the wells. Following filtration, the residue is washed twice with 200 μl of 0.5% orthophosphoric acid. Once the filters have dried, 25 μl of Microscint 20 scintillant is added, and then counted on a Packard Topcount for 30 seconds.
The % inhibition ofthe CDK2 activity is calculated and plotted in order to determine the concentration of test compound required to inhibit 50% ofthe CDK2 activity (ICso).
The compounds of Examples 3 to 19, 21 to 76, 78, 80, 81 and 84 to 87 each have IC50 values of less than lOOμM or provide at least 50% inhibition ofthe CDK2 activity at a concentration of 50 μM.
PHARMACEUTICAL FORMULATIONS
EXAMPLE 89 (ϊ) Tablet Formulation
A tablet composition containing a compound ofthe formula (I) is prepared by mixing 50mg ofthe compound with 197mg of lactose (BP) as diluent, and 3mg magnesium stearate as a lubricant and compressing to form a tablet in known manner.
(if) Capsule Formulation
A capsule formulation is prepared by mixing lOOmg of a compound ofthe formula (I) with lOOmg lactose and filling the resulting mixture into standard opaque hard gelatin capsules.
EXAMPLE 90
Determination of Antifungal Activity
The antifungal activity ofthe compounds ofthe formula (I) is determined using the following protocol .
The compounds are tested against a panel of fungi including Candida parpsilosis, Candida tropicalis, Candida albicans-ATCC 36082 and Cryptococcus neoformans. The test organisms are maintained on Sabourahd Dextrose Agar slants at 4 °C. Singlet suspensions of each organism are prepared by growing the yeast overnight at 27 °C on a rotating drum in yeast-nitrogen base broth (YNB) with amino acids (Difco, Detroit, Mich.), pH 7.0 with 0.05 morpholine propanesulphonic acid (MOPS). The suspension is then centrifuged and washed twice with 0.85% NaCl before sonicating the washed cell suspension for 4 seconds (Branson Sonifier, model 350, Danbury, Conn.). The singlet blastospores are counted in a haemocytometer and adjusted to the desired concentration in 0.85% NaCl.
The activity ofthe test compounds is determined using a modification of a broth microdilution technique. Test compounds are diluted in DMSO to a 1.0 mg/ml ratio then diluted to 64 μg/ml in YNB broth, pH 7.0 with MOPS (Fluconazole is used as the control) to provide a working solution of each compound. Using a 96-well plate, wells 1 and 3 through 12 are prepared with YNB broth, ten fold dilutions ofthe compound solution are made in wells 2 to 11 (concentration ranges are 64 to 0.125 μg/ml). Well 1 serves as a sterility control and blank for the spectrophotometric assays. Well 12 serves as a growth control. The microtitre plates are inoculated with 10 μl in each of well 2 to 11 (final inoculum size is 104 organisms/ml). Inoculated plates are incubated for 48 hours at 35 °C. The MIC values are determined spectrophotometrically by measuring the absorbance at 420 nm (Automatic Microplate Reader, DuPont Instruments, Wilmington, Del.) after agitation ofthe plates for 2 minutes with a vortex-mixer (Norte-Genie 2 Mixer, Scientific
Industries, Inc., Bolemia, Ν.Y.). The MIC endpoint is defined as the lowest drag concentration exhibiting approximately 50% (or more) reduction ofthe growth compared with the control well. With the turbidity assay this is defined as the lowest drug concentration at which turbidity in the well is <50%of the control (IC50). Minimal Cytolytic Concentrations (MCC) are determined by sub-culturing all wells from the 96-well plate onto a Sabourahd Dextrose Agar (SDA) plate, incubating for 1 to 2 days at 35 °C and then checking viability.
EXAMPLE 89
Protocol for the Biological Evaluation of Control of in vivo Whole Plant Fungal Infection
Compounds ofthe formula (I) are dissolved in acetone, with subsequent serial dilutions in acetone to obtain a range of desired concentrations. Final treatment volumes are obtained by adding 9 volumes of 0.05% aqueous Tween-20 ™ or 0.01% Triton X-100™, depending upon the pathogen.
The compositions are then used to test the activity ofthe compounds ofthe invention against tomato blight (Phytophthora infestans) using the following protocol. Tomatoes (cultivar Rutgers) are grown from seed in a soil-less peat-based potting mixture until the seedlings are 10-20 cm tall. The plants are then sprayed to run-off with the test compound at a rate of 100 ppm. After 24 hours the test plants are inoculated by spraying with an aqueous sporangia suspension of Phytophthora infestans, and kept in a dew chamber overnight. The plants are then transferred to the greenhouse until disease develops on the untreated control plants.
Similar protocols are also used to test the activity ofthe compounds ofthe invention in combatting Brown Rust of Wheat (Puccinia), Powdery Mildew of Wheat (Ervsiphe vraminis), Wheat (cultivar Monon), Leaf Blotch of Wheat (Septoria tritici), and Glume Blotch of Wheat (Leptosphaeria nodorum).
Equivalents
The foregoing examples are presented for the purpose of illustrating the invention and should not be construed as imposing any limitation on the scope ofthe invention. It will readily be apparent that numerous modifications and alterations may be made to the specific embodiments ofthe invention described above and illustrated in the examples without departing from the principles underlying the invention. All such modifications and alterations are intended to be embraced by this application.

Claims

1. A compound of the formula (I) for use in the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase:
Figure imgf000106_0001
wherein
9 9 9
A is a group R or CH2-R where R is a carbocyclic or heterocyclic group having from 3 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O; R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8,
CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2), C(X2)X1, X1C(X )X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Cμs hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Ct-s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NR°, X!C(X2), C(X2)X1 or X1C(X2)X1; Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC;
R7 is selected from hydrogen and a C1-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe Ci-β hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C(X2)X* or X'CCX^X1; R is selected from R and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR R or NR R may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N- [(morpholin-4-yl)phenyl- 1 H- indazole-3 -carboxamide and N-[4-(acetylaminosulphonyl)phenyl-lH- indazole-3-carboxamide.
2. A compound for use according to claim 1 wherein A is a group R2.
3. A compound for use according to any one ofthe preceding claims wherein the carbocyclic or heterocyclic group R2 is other than a bridged polycyclic group
4. A compound for use according to any one ofthe preceding claims wherein
R is a carbocyclic group.
5. A compound for use according to claim 4 wherein the carbocyclic group is a benzene ring.
6. A compound for use according to any one ofthe preceding claims wherein the group R2 bears no substituents other than the group B.
7. A compound for use according to any of claims 1 to 5 wherein the group R is substituted by one or more substituents R10 selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X^CX2), C(X2)X1, X1C(X2)X1, S, SO, SO2,
NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C^s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe -s hydrocarbyl group may optionally be replaced by O, S, SO, SO , NRC, X1C(X2), CCX^X1 or X^fX^X1;
Rc is hydrogen or CM hydrocarbyl; and X1 is O, S or NRC and X2 is =O, =S or =NRC.
8. A compound for use according to claim 7 wherein R10 is selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, amino; a group Ra-Rb wherein Ra is a bond, O, CO, XJC(X2), C(X2)X1, X1C(X2)X1, S, SO, SO2, NR°, SO2NRc or NRcSO2; and Rb is selected from hydrogen and a C w hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino and wherein one or more carbon atoms ofthe Ci-s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X'CfX2), C(X2)Xl or X1C(X2)X1; Rc is hydrogen or CM hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC.
9. A compound for use according to claim 7 or claim 8 wherein the group R2 is substituted by 1, 2, 3 or 4 groups R10.
10. A compound for use according to any one ofthe preceding claims wherein R1 is other than hydrogen.
11. A compound for use according to claim 10 wherein R1 is selected from S SOO22NNRR77RR88,, CCOONNRR77RR88,, NNRR77RR99 a anndd carbocyclic and heterocyclic groups having from 3 to 7 ring members.
12. A compound per se ofthe formula (II):
Figure imgf000109_0001
wherein
E is a group R , 1l2Δ or CH2-R12a where R12 is a substituted or unsubstituted, non-bridged, carbocyclic or heterocyclic group having from 3 to 12 ring members, other than a diazacycloalkyl moiety, and R12a is an unsubstituted or substituted aryl or heteroaryl group having from 5 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, XJC(X2), C(X2)X!, X1C(X2)XI, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a Cι-8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Ci-s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C(X2)X1 or X^CX^X1 ;
Rc is hydrogen or C hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NR°; R7 is selected from hydrogen and a Cι_8 hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe -s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C(X2)X* or X1C(X2)X1; R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for the groups R12 and R12a can be one or more substituent groups R10 selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-R wherein Ra is a bond, O, CO, X1C(X2), C(X2)X1, X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a -s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and" wherein one or more carbon atoms ofthe Cι„8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XlC(X2), C(X2)X* or X^CX^X1;
R° is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC; with the provisos that:
(a) when R12 is an azacycloalkyl or diazacycloalkyl group, at least one nitrogen atom ofthe azacycloalkyl or diazacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group;
(b) when E is a substituted phenyl group, the or each substituent is other than a 5-7 membered non-aromatic ring (such as cyclohexyl) having attached thereto a diazacycloalkyl moiety (such as piperazine), a nitrogen atom of which moiety bears an aryl or heteroaryl substituent; and
(c) R12 and R12a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
(i) N- [(morpholin-4-yl)phenyl- 1 H-indazole-3 -carboxamide;
(ii) N-[4-(acetylaminosulphonyl)phenyl-lH-indazole-3-carboxamide;
1 V R
(iii) compounds wherein E is phenyl, R is NR R and B is a group
-CH(CH2OH)CH2-; (iv) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy;
(v) compounds wherein E is unsubstituted pyridyl, B is a bond and R1 is hydrogen;
(vi) compounds wherein E is phenyl substituted with one or more of alkyl, alkoxy, alkylsulphanyl, alkylsulphinyl other than met -alkylsulphinyl, alkylsulphonyl other than et -alkylsulphonyl, halogen, nitro and trihalomethyl, B is a bond, and R1 is hydrogen;
(vii) compounds wherein E is a thiophene group bearing a 3- aminocarbonyl substituent; (viii) the compound wherein E is unsubstituted phenyl oτpara- methoxyphenyl, and each of R3 to R6 is hydrogen; (ix) N-4-methylbenzyl-lH-indazole-3-carboxamide; (x) compounds wherein R3, R5 and R6 are each hydrogen, R4 is methyl and A is unsubstituted benzyl, unsubstituted phenyl, methylphenyl, etα- τrifluoromethylphenyl, and ortfto-methoxyphenyl; (xi) compounds in which E is a 2,2-dimethyl- 1 ,3-dioxane ring; (xii) compounds containing a benzene ring substituted by a pair of meta- oriented carboxamido moieties; (xiii) compounds wherein E is a trisubstituted phenyl group and two of the substitutents are fluoro and chloro respectively.
13. A compound accoding to claim 12 wherein E-B-R1 is other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
14. A compound according to claim 12 wherein E-B-R1 is other than a saturated azabicyclic moiety or an imidazolyl moiety.
15. A compound according to claim 12 wherein when E-B-R1 is an unsubstituted phenyl group, R3 to R6 are each other than a group Ra-Rb wherein Ra is a bond and Rb is a substituted C3-C8 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
16. A compound per se of the formula (III) :
Figure imgf000112_0001
wherein
G is a group R14 or CH2-R14 where R14 is a carbocyclic group having from 3 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R13 is a group selected from SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R , R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, XlC(X2),
Figure imgf000113_0001
XlC X2)X S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a C^s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the -s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^Q 2), C(X2)Xl or X1C(X2)X1; Rc is hydrogen or CM hydrocarbyl;
X1 is O, S or NR° and X2 is =O, =S or =NR°; R is selected from hydrogen and a C^s hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C\.% hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^O 2), C(X2)X! or XlC(X2)Xl;
R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members; R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; but excluding the compounds N-[(morpholin-4-yl)phenyl-lH- indazole-3 -carboxamide and N- [4-(acetylaminosulphonyl)phenyl- 1 H- indazole-3-carboxamide; and further excluding; (i) compounds wherein A is phenyl, R1 is NR7R8 and B is a group - CH(CH2OH)CH2-;
(ii) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy.
17. A compound per se or compound for use according to any one ofthe preceding claims wherein B is a bond.
18. A compound per se or compound for use according to any one of claims 1 to 16 wherein B is an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O.
19. A compound per se or compound for use according to claim 18 wherein the linker group has a linking chain length of 1 atom.
20. A compound per se or compound for use according to claim 18 or claim 19 wherein the atoms defining the linking chain length are all carbon atoms.
21. A compound per se or compound for use according to any one of claims 18 to 20 wherein the linker group is a straight chain group.
22. A compound per se or compound for use according to claim 21 wherein B is a group (CH2)n wherein n is 1, 2 or 3.
23. A compound per se or compound for use according to any one ofthe preceding claims wherein R is hydrogen.
24. A compound per se or compound for use according to any one ofthe preceding claims wherein R3 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and Ra-Rb.
25. A compound per se or compound for use according to claim 24 wherein R3 is hydrogen, Ci-g alkyl, fluorine or chlorine.
26. A compound per se or compound for use according to any one ofthe preceding claims wherein R5 is hydrogen or a group selected from halogen, hydroxy, cyano, trifluoromethyl, amino and Ra-Rb.
27. A compound er se or compound for use according to claim 26 wherein R5 is hydrogen, -6 alkyl, fluorine or chlorine.
28. A compound per se or compound for use according to any one ofthe preceding claims wherein R and R are both hydrogen.
29. A compound per se or compound for use according to any one ofthe preceding claims wherein R6 is selected from hydrogen, methyl, amino, fluorine and chlorine.
30. A compound per se or compound for use according to claim 29 wherein R6 is selected from hydrogen and amino.
31. A compound per se or compound for use according to claim 30 wherein R6 is hydrogen.
32. A compound per se or compound for use according to any one ofthe preceding claims wherein R4 is selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a group Ra-Rb.
33. A compound per se or compound for use according to claim 32 wherein R4 is selected from hydrogen, halogen, a heterocyclic group and a group R -Rb wherein Ra is a bond, O, CO, X1C(X2), C(X2)X1, X1C(X2)X1 5 S, SO, SO2, NRC, SO2NRc or NR°SO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 5 to 10 ring members, and a CM hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, monocyclic carbocyclic and heterocyclic groups having from 5 to 10 ring members and wherein one or more carbon atoms ofthe Ci- s hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRQ, X1C(X2), C(X2)XJ or X1C(X2)X1.
34. A compound per se or compound for use according to claim 33 wherein R4 is selected from hydrogen, halogen, a heterocyclic group, a group O-Het where Het is a heterocyclic groups having from 5 to 10 ring members, CM alkyl, C 6 alkoxy, C(O)NRcRb and SO2NRcRb wherein Rb is hydrogen or Cι-6 alkyl.
35. A compound of the formula (IN) :
Figure imgf000116_0001
wherein R3 to R8, G and B are as defined in any one ofthe preceding claims.
1 P.
36. A compound according to claim 35 wherein R and R are selected from hydrogen and CM alkyl or R7 and R8 together with the nitrogen atom form a saturated five or six membered heterocyclic ring having one or two heteroatoms.
37. A compound according to claim 36 wherein R7 and R8 together with the nitrogen atom form a saturated heterocyclic ring selected from morpholino, piperidino, piperazino and pyrrolidino.
38. A compound according to claim 35 wherein R7 is hydrogen and R8 is hydrogen or methyl.
39. A compound ofthe formula (N):
Figure imgf000117_0001
wherein R • 3 to R , G and B are as defined in any one ofthe preceding claims.
40. A compound ofthe formula (Nl):
Figure imgf000117_0002
wherein R3 to R6 and G are as defined in any one ofthe preceding claims and Het' is a heterocylic group having from 3 to 7 ring members, but excluding the compound Ν- [(morpholin-4-yl)phenyl] - 1 H-indazole-3 - carboxamide.
41. A compound according to claim 40 wherein a carbon atom ofthe heterocyclic group Het' is linked to the group G.
42. A compound according to claim 40 or claim 41 wherein the group Het' is a five membered heteroaryl ring containing 2 or more nitrogen ring members.
43. A compound according to claim 42 wherein the group Het' is selected from tetrazolyl, pyrrolidonyl (e.g.N-pyrrolidonyl), oxazolyl and imidazolyl.
44. A compound ofthe formula (Nil):
Figure imgf000118_0001
wherein R >3 * to, R , R , G and B are as hereinbefore defined.
45. A compound according to claim 44 wherein R7 is selected from hydrogen and CM alkyl and R9 is selected from hydrogen, CM alkyl and CM alkanoyl such as acetyl.
46. A compound according to any one of claims 35 to 46 wherein G is a group R14 wherein R14 is an aryl group having six ring members and B is a bond or a methylene group.
47. A compound ofthe formula (NIII):
Figure imgf000118_0002
wherein R3 to R and Rb are as defined in any one ofthe preceding claims and R11 represents hydrogen or one or more substituents selected from halogen, C alkyl, C alkoxy, trifluoromethyl and trifluoromethoxy.
48. A compound according to claim 47 wherein the group SO2Rb is attached to the røetα-position ofthe benzene ring.
49. A compound according to claim 47 wherein the group SO Rb is attached to thepαrø-position ofthe benzene ring.
50. A compound according to any one of claims 47 to 49 wherein R11 is hydrogen.
51. A compound according to any one of claims 47 to 50 wherein R is C alkyl.
52. A compound according to claim 51 wherein R is methyl.
53. A compound of the formula (IX) :
Figure imgf000119_0001
wherein
J is a group R15 or CH2-R15a where R15 is a substituted or unsubstituted, non-bridged heterocyclic group having from 5 to 12 ring members, other than a diazacycloalkyl moiety, and R15a is an unsubstituted or substituted aryl or heteroaryl group having from 5 to 12 ring members;
B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R1 is hydrogen when R15a is aryl or, when R15a is other than aryl, R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, XlC(X2), C(X2)Xl, X1C(X2)X1, S, SO, SO2, NR°, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a CM hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Ci-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X1C(X2), CCX^X1 or X1C(X2)X1;
Rc is hydrogen or C hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC; R7 is selected from hydrogen and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-Ci-4 hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe CM hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X'C 2), C(X2)X* or X1C(X2)X1; R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for the groups R15 and R15a can be one or more substituent groups R selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2),
Figure imgf000120_0001
S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a CM hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X!C(X2), C(X2)Xl or X^ X^X1; provided that when R15a is aryl it is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O, by a group selected from SO2Rb, SO2NR7R8,
CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
Rc is hydrogen or C hydrocarbyl; X1 is O, S or NRC and X2 is =O, =S or =NRC; with the provisos that:
(a) when R15 is an azacycloalkyl group and all of R to R6 are hydrogen, at least one nitrogen atom ofthe azacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group;
(b) R15 and R15a are each other than a substituted or unsubstituted imidazole moiety; but excluding the following:
(i) compounds wherein R3 and R6 are both hydrogen and R4 and R5 are both methoxy;
(ii) compounds wherein R3 to R6 are all hydrogen, J is unsubstituted pyridyl or pyridylmethyl, B is a bond and R1 is hydrogen;
(iii) compounds wherein J is phenyl substituted with one or more of alkyl, alkoxy, alkylsulphanyl, alkylsulphinyl other than røetα-alkylsulphinyl, alkylsulphonyl other than met -alkylsulphonyl, halogen, nitro and trihalomethyl, B is a bond, and R1 is hydrogen; (iv) compounds wherein J is a thiophene group bearing a 3- aminocarbonyl substituent;
(v) the compound wherein J is unsubstituted phenyl ox par a- methoxyphenyl, and each of R3 to R6 is hydrogen; (vi) N-4-methylbenzyl-lH-indazole-3-carboxamide;
(vii) compounds wherein R3, R5 and R6 are each hydrogen, R4 is methyl and A is unsubstituted benzyl, unsubstituted phenyl, methylphenyl, metar trifluoromethylphenyl, and ørt ?σ-methoxyphenyl; (viii) compounds in which J is a 2,2-dimethyl- 1 ,3-dioxane ring; (ix) compounds containing a benzene ring substituted by a pair of meta- oriented carboxamido moieties; and
(x) compounds wherein J is a trisubstituted phenyl group and two ofthe substituents are fluoro and chloro respectively.
54. A compound ofthe formula (X):
Figure imgf000122_0001
wherein
L is a group R16 or CH2-R16 where R16 is a substituted or unsubstituted heteroaryl group other than imidazole, the heteroaryl group having from 5 to 12 ring members, at least one of which is nitrogen; B is a bond or an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O;
R1 is hydrogen or a group selected from SO2Rb, SO2NR7R8,
CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to
7 ring members; R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2),
Figure imgf000123_0001
X1C(X2)X1, S, SO, SO2, NRC, SO2NR° or NR°SO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C^ hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, XJC(X2), C(X2)X* or X1C(X2)X1, provided that R4 and R5 cannot both be methoxy;
Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC;
R is selected from hydrogen and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X!C(X2), C(X2)X* or X1C(X2)X1;
R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for R16 can be one or more substituent groups R10 selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, XlC(X2),
Figure imgf000123_0002
X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X!C(X2), C(X2)XJ or X1C(X2)X1;
Rc is hydrogen or CM hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC; but excluding compounds wherein all of R3 to R6 are hydrogen and L-B-R1 defines an unsubstituted pyridyl or pyridylmethyl group.
55. A compound according to claim 53 or claim 54 wherein the compound is other than a compound in which J is unsubstituted pyridyl or pyridylmethyl, B is a bond and R1 is hydrogen.
56. A compound according to claim 54 having the formula (XI):
Figure imgf000124_0001
in which R17 is hydrogen, B-R1 or R10, and wherein R4, B-R1 and R10 are as hereinbefore defined, provided that at least one of R4 and R17 is other than hydrogen.
57. A compound according to claim 56 having the formula (XII):
Figure imgf000125_0001
58. A compound according to claim 54 having the formula (XIII):
Figure imgf000125_0002
in which R17 is hydrogen, B-R1 or R 10
59. A compound according to claim 54 having the formula (XIN):
Figure imgf000125_0003
in which R17 is hydrogen, B-R1 or R10.
60. A compound ofthe formula (XN):
Figure imgf000125_0004
wherein on OΛ 9Λ
M is a group R or CH2-R where R is an aryl group having from 6 to 12 ring members and being optionally substituted by one or two substituent groups R10 which may be the same or different;
1 ft
R is selected from hydrogen, halogen, and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R19 is selected from hydrogen and amino, provided that at least one of R18 and R19 is other than hydrogen;
R10 is selected from halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2),
C(X2)X1, X1C(X2)X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^O 2), C(X2)Xl or X'CCX^X1; provided that the aryl group R20 is not substituted either directly, or via an acyclic linker group having a linking chain length of up to 3 atoms selected from C, N, S and O, by a group selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9 and carbocyclic and heterocyclic groups having from 3 to 7 ring members;
Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NR° and X2 is =O, =S or =NRC.
61. A compound according to claim 60 wherein R18 is halogen, especially iodine or chlorine, and R19 is hydrogen.
62. A compound ofthe formula (XVI):
Figure imgf000127_0001
wherein
Q is an optionally substituted non-bridged non-aromatic heterocyclic group having from 5 to 7 ring members of which at least one is a nitrogen atom, the group being other than a diazacycloalkyl group;
R3, R4, R5 and R6 are the same or different and are each selected from hydrogen, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO, X1C(X2),
Figure imgf000127_0002
X^ ^X1, S, SO, SO2, NRC, SO2NRc or NRcSO2; and Rb is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 12 ring members, and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms of the Cι-8 hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^QI2), CCX^X1 or X^CX^X1;
Rc is hydrogen or C hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NRC;
R7 is selected from hydrogen and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-C1.4 hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NRC, X^O 2), C(X2)Xl or X1C(X2)X1; R8 is selected from R7 and carbocyclic and heterocyclic groups having from 3 to 12 ring members;
R9 is selected from R8, COR8 and SO2R8; or NR7R8 or NR7R9 may each form a heterocyclic group having from 5 to 12 ring members; and the optional substituents for the group Q can be one or more
91
(preferably up to 2, for example 1) substituent groups R selected from SO2Rb, SO2NR7R8, CONR7R8, NR7R9, halogen, hydroxy, trifluoromethyl, cyano, nitro, carboxy, amino, carbocyclic and heterocyclic groups having from 3 to 12 ring members; a group Ra-Rb wherein Ra is a bond, O, CO,
Figure imgf000128_0001
S, SO, SO2, NRC, SO2NRc or NRcSO2; and R is selected from hydrogen, carbocyclic and heterocyclic groups having from 3 to 7 ring members, and a C hydrocarbyl group optionally substituted by one or more substituents selected from hydroxy, oxo, halogen, cyano, nitro, amino, mono- or di-CM hydrocarbylamino, carbocyclic and heterocyclic groups having from 3 to 12 ring members and wherein one or more carbon atoms ofthe C hydrocarbyl group may optionally be replaced by O, S, SO, SO2, NR°, X^Q 2), C(X2)X or X1C(X2)X1; Rc is hydrogen or CM hydrocarbyl;
X1 is O, S or NRC and X2 is =O, =S or =NR°; provided that when Q is an azacycloalkyl group and R3 to R6 are all hydrogen, at least one nitrogen atom ofthe azacycloalkyl or diazacycloalkyl group is substituted by an acyl, sulphinyl or sulphonyl group.
63. A compound as defined in any one ofthe preceding claims wherein said compound does not contain a benzene ring substituted by a pair of meta- oriented carboxamido moieties.
64. A compound according to claim 53 or claim 54 wherein J-B-R1 and L-B-R1 are other than a diazine or triazine substituted by a monocyclic pyrazolyl group or a bicyclic fused pyrazolyl group.
65. A compound according to any one of claims 53, 54 and 62 wherein J-B-R1 and L-B-R1 are other than a saturated azabicyclic moiety or an imidazolyl moiety.
66. A compound according to claim 53 or claim 59 wherein when J-B-R1 is an unsubstituted phenyl group, R3 to R6 are each other than a group Ra-Rb wherein Ra is a bond and Rb is a substituted C3-C8 hydrocarbyl group having two or more substituents, one of which contains an unsubstituted or substituted amino group.
67. A compound selected from:
1 H-Indazole-3 -carboxylic acid (4-methylsulphamoy lmethyl-phenyl)-amide ; lH-Indazole-3 -carboxylic acid [3-(lH-tetrazol-5-yl)-phenyl]-amide; lH-Indazole-3 -carboxylic acid [4-(acetylamino-methyl)-phenyl]-amide; lH-Indazole-3 -carboxylic acid [4-(2-oxo-pyrrolidin-l-yl)-phenyl]-amide; lH-Indazole-3 -carboxylic acid (3-oxazol-5-yl-phenyl)-amide; lH-Indazole-3 -carboxylic acid [4-(lH-imidazol-4-yl)-phenyl]-amide; lH-Indazole-3 -carboxylic acid (3-methanesulphonyl-phenyl)-amide; lH-Indazole-3 -carboxylic acid [4-(morpholine-4-sulphonyl)-phenyl]-amide;
5-Iodo-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenyl)-amide;
5-Iodo- 1 H-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide;
5-Iodo-lH-indazole-3-carboxylic acid (3-methanesulphonyl-phenyl)-amide; 5 -Iodo-1 H-indazole-3 -carboxylic acid [4-(acetylamino-methyl)-phenyl]- amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid (4-sulphamoyl-phenyl)-amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide; 5-thiophen-2-yl-lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenyl)-amide;
5-(3,5-dimethyl-isoxazol-4-yl)-lH-indazole-3-carboxylic acid (4- methylsulphamoylmethyl-phenyl)-amide; 5 -furan-2-yl- 1 H-indazole-3 -carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide; and
5 -benzofuran-2-yl-l H-indazole-3 -carboxylic acid (4- methylsulphamoylmethyl-phenyl)-amide;
N-phenyl-5-iodo- 1 H-indazole-3-carboxamide; 5 -morpholin-4-yl-l H-indazole-3 -carboxylic acid phenylamide;
5-chloro-lH-indazole-3-carboxylic acid (5-nitro-pyridin-2-yl)-amide;
1 H-indazole-3 -carboxylic acid (4-sulphamoyl-phenyl)-amide;
5 -thiophen-2-yl-l H-indazole-3 -carboxylic acid (4- methylsulphamoylmethyl-phenyl)-amide; 5-thiazol-2-yl-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide;
4-[(5-iodo-lH-indazole-3-carbonyl)-amino]-piperidine-l-carboxylic acid ethyl ester;
1 H-indazole-3 -carboxylic acid [4-(thiazol-2-ylsulphamoyl)-phenyl]-amide; 5 -phenyl- 1 H-indazole-3 -carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid [4-(methanesulphonylamino-methyl)- phenyl] -amide;
4- [(5-nitro- 1 H-indazole-3-carbonyl)-amino] -piperidine- 1 -carboxylic acid ethyl ester;
5-chloro-lH-indazole-3-carboxylic acid (l-benzyl-pyrrolidin-3-yl)-amide;
4- [(5 -chloro- 1 H-indazole-3 -carbonyl)-amino] -piperidine- 1 -carboxylic acid ethyl ester;
5-iodo- lH-indazole-3-carboxylic acid (6-methoxy-pyridin-3-yl)-amide; 5-iodo-lH-indazole-3-carboxylic acid pyridin-3-yl-amide;
5-iodo-lH-indazole-3-carboxylic acid quinolin-3-ylamide; 5-iodo-lH-indazole-3-carboxylic acid (tetrahydro-pyran-4-yl)-amide;
5 -chloro- 1 H-indazole-3 -carboxylic acid ( 1 -methy l-piperidin-4-yl)-amide;
5-iodo-lH-indazole-3-carboxylic acid (2-chloro-pyridin-3-yl)-amide;
5-chloro- 1 H-indazole-3 -carboxylic acid benzylamide; 5 -chloro- 1 H-indazole-3 -carboxylic acid 4-(4-methyl-piperazin-l-yl)- benzylamide;
5 -chloro- 1 H-indazole-3 -carboxylic acid pyridin-3-ylamide;
5 -iodo-1 H-indazole-3 -carboxylic acid (6-cyano-pyridin-3-yl)-amide;
5 -chloro- 1 H-indazole-3 -carboxylic acid phenylamide; 5-iodo-lH-indazole-3-carboxylic acid (6-methyl-pyridazin-3-yl)-amide;
5-chloro-lH-indazole-3-carboxylic acid (5-ethyl-[l,3,4]thiadiazol-2-yl)- amide;
5-iodo-lH-indazole-3-carboxylic acid (4-morpholin-4-yl-phenyl)-amide;
5-iodo-lH-indazole-3-carboxylic acid (2-oxo-l,2-dihydro-pyridin-3-yl)- amide;
1 H-indazole-3 -carboxylic acid (4-morpholin-4-yl-phenyl)-amide;
5 -nitro- 1 H-indazole-3 -carboxylic acid phenylamide;
5 -iodo- 1 H-indazole-3 -carboxylic acid (6-chloro-pyridin-3 -yl)-amide;
4- [( 1 H-indazole-3 -carbonyl)-amino] -piperidine- 1 -carboxylic acid tert-butyl ester;
5-iodo-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide;
5-iodo-lH-indazole-3-carboxylic acid (6-acetylamino-pyridin-3-yl)-amide;
5 -amino- 1 H-indazole-3 -carboxylic acid phenylamide;
5 -iodo- 1 H-indazole-3 -carboxylic acid (4-methylaminosulphonylmethyl- phenyl)-amide;
5-amino-lH-indazole-3-carboxylic acid (4-sulphamoyl-phenyl)-amide;
7-amino-l H-indazole-3 -carboxylic acid (4-sulphamoyl-phenyl)-amide;
5-[3-(2-chloro-ethyl)-ureido]-lH-indazole-3-carboxylic acid (4- methylsulphamoyl-methyl-phenyι)-amide; 5-nitro-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl-phenyl)- amide; 5-amino-lH-indazole-3-carboxylic acid (4-methylsulphamoylmethyl- phenyl)-amide;
5-iodo-lH-indazole-3-carboxylic acid piperidin-4-ylamide
5-chloro-lH-indazole-3-carboxylic acid [4-(acetylamino-methyl)-phenyl]- amide; lH-indazole-3-carboxylic acid [l-(2,2,2 trifluoro-acetyl)-Piperidin-4- yl]- amide;
1 H-indazole-3 -carboxylic acid piperidin-4-ylamide;
1 H-indazole-3 -carboxylic acid (l-acetyl-piperidin-4-yl)-amide; 1 H-indazole-3 -carboxylic acid (l-methanesulphonyl-piperidin-4-yl)-amide;
1 H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide;
4-bromo-l H-indazole-3 -carboxylic acid (4-fluoro-phenyl)-amide;
5-nitro-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide;
5-amino-lH-indazole-3-carboxylic acid (4-fluorophenyl)-amide; 5-amino-4-bromo- 1 H-indazole-3 -carboxylic acid (4-fluorophenyl)-amide;
5-methyl-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide;
6-bromo-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide;
5-chloro-lH-indazole-3-carboxylic acid (4-morpholin-4-yl-phenyl)-amide;
5-chloro-lH-indazole-3-carboxylic acid [3-(lH-tetrazol-5-yl)-phenyl]- amide;
5-iodo-lH-indazole-3-carboxylic acid (4-pyrrolidin-l-ylmethyl-phenyl)- amide;
5-chloro-lH-indazole-3-carboxylic acid [4-(tbiazol-2-ylsulphamoyl)- phenyl] -amide; 5-chloro-lH-indazole-3-carboxylic acid (4-fluoro-phenyl)-amide;
3-[(5-chloro-lH-indazole-3-carbonyl)-amino]-pyrrolidine-l-carboxylic acid methyl ester;
5-fluoro-lH-indazole-3-carboxylic acid phenylamide;
5-morpholin-4-yl-lH-indazole-3-carboxylic acid (6-chloro-pyridin-3-yl)- amide;
1 H-indazole-3 -carboxylic acid (6-chloro-pyridin-3-yl)-amide; 5 -phenethyl-1 H-indazole-3 -carboxylic acid phenylamide; 5-(l,l-dioxo-llambda*6*-isothiazolidin-2-yl)-lH-indazole-3-carboxylic acid phenylamide;
5 -biphenyl-2-yl-l H-indazole-3 -carboxylic acid phenylamide; 5-pyrrolidin-l-yl-lH-indazole-3-carboxylic acid phenylamide;
5-chloro-lH-indazole-3-carboxylic acid [5-(tetrahydro-furan-2-yl)-
[1 ,3,4]thiadiazol-2-yl]-amide; and
5-nitro-lH-indazole-3-carboxylic acid (3-methanesulphonyl-phenyl)-amide
68. A compound according to any one ofthe preceding claims in the form of a salt or solvate.
69. A compound according to any one ofthe preceding claims in the form of an N-oxide.
70. A compound according to any one of claims 12 to 69 for use in medicine.
71. A compound according to any one of claim 12 to 69 for use in the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
72. A pharmaceutical composition comprising a compound as defined in anyone of claims 12 to 69 and a pharmaceutically acceptable carrier.
73. The use of a compound according to any one of claims 1 to 69 for the manufacture of a medicament for the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase.
74. A method for the prophylaxis or treatment of a disease state or condition mediated by a cyclin dependent kinase, which method comprises administering to a subject in need thereof a compound as defined in any one of claims 1 to 69.
75. A method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, which method comprises administering to the mammal a compound as defined in any one of claims 1 to 69 in an amount effective in inhibiting abnormal cell growth.
76. A method for treating a disease or condition comprising or arising from abnormal cell growth in a mammal, the method comprising administering to the mammal a compound as defined in any one of claims 1 to 69 in an amount effective to inhibit CDK2 activity.
77. A method of inhibiting a cyclin dependent kinase, which method comprises contacting the kinase with a kinase-inhibiting compound as defined in any one of claims 1 to 69.
78. A method of modulating a cellular process (for example cell division) by inhibiting the activity of a cyclin dependent kinase using a compound as defined in any one of claims 1 to 69.
79. A compound according to any one of claims 1 to 69 for use as an antifungal agent.
PCT/GB2003/003491 2002-08-10 2003-08-08 1h-indazole-3-carboxamide compounds as cyclin dependent kinases (cdk) inhibitors WO2004014864A1 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
EP03784286A EP1534685A1 (en) 2002-08-10 2003-08-08 1h-indazole-3-carboxamide compounds as cyclin dependent kinases (cdk) inhibitors
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