WO2004011938A3 - Method and device for screening molecules in cells - Google Patents

Method and device for screening molecules in cells Download PDF

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Publication number
WO2004011938A3
WO2004011938A3 PCT/FR2003/002298 FR0302298W WO2004011938A3 WO 2004011938 A3 WO2004011938 A3 WO 2004011938A3 FR 0302298 W FR0302298 W FR 0302298W WO 2004011938 A3 WO2004011938 A3 WO 2004011938A3
Authority
WO
WIPO (PCT)
Prior art keywords
cell
reagent
support
device
method
Prior art date
Application number
PCT/FR2003/002298
Other languages
French (fr)
Other versions
WO2004011938B1 (en
WO2004011938A2 (en
Inventor
Francois Chatelain
Yves Fouillet
Brigitte Fouque
Alexandra Fuchs
Beatrice Schaack
Original Assignee
Francois Chatelain
Commissariat Energie Atomique
Yves Fouillet
Brigitte Fouque
Alexandra Fuchs
Beatrice Schaack
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to FR0209326 priority Critical
Priority to FR0209326A priority patent/FR2842747B1/en
Application filed by Francois Chatelain, Commissariat Energie Atomique, Yves Fouillet, Brigitte Fouque, Alexandra Fuchs, Beatrice Schaack filed Critical Francois Chatelain
Publication of WO2004011938A2 publication Critical patent/WO2004011938A2/en
Publication of WO2004011938A3 publication Critical patent/WO2004011938A3/en
Publication of WO2004011938B1 publication Critical patent/WO2004011938B1/en

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5023Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5032Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on intercellular interactions
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5058Neurological cells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00351Means for dispensing and evacuation of reagents
    • B01J2219/00364Pipettes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00351Means for dispensing and evacuation of reagents
    • B01J2219/00364Pipettes
    • B01J2219/00367Pipettes capillary
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00351Means for dispensing and evacuation of reagents
    • B01J2219/00378Piezo-electric or ink jet dispensers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00646Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports
    • B01J2219/0065Making arrays on substantially continuous surfaces the compounds being bound to beads immobilised on the solid supports by the use of liquid beads
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
    • C40B60/00Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027General features of the devices
    • G01N2035/1034Transferring microquantities of liquid
    • G01N2035/1046Levitated, suspended drops

Abstract

The invention concerns a method for reacting a reagent R with a cell C, which consists in: setting the cell C on a support S comprising a substantially planar surface, in the form of an aqueous drop on said surface; covering the planar surface of the support S whereon the aqueous drop has been set containing the cell C with a separation film F, allowing through gases and preventing the aqueous drops set on the support S from evaporating, F being non-miscible with the reagent R; triggering the reaction between the reagent R and the cell C by introducing the reagent R in the aqueous drop containing the cell C. The invention also concerns a device for implementing said procedure and its applications.
PCT/FR2003/002298 2002-07-23 2003-07-21 Method and device for screening molecules in cells WO2004011938A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR0209326 2002-07-23
FR0209326A FR2842747B1 (en) 2002-07-23 2002-07-23 Method and device for screening molecules in cells

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
EP03750828A EP1525472A2 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells
AU2003269041A AU2003269041A1 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells
JP2004523860A JP2005533509A (en) 2002-07-23 2003-07-21 Method and apparatus for screening molecules in cells
CA 2492933 CA2492933A1 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells

Publications (3)

Publication Number Publication Date
WO2004011938A2 WO2004011938A2 (en) 2004-02-05
WO2004011938A3 true WO2004011938A3 (en) 2004-08-05
WO2004011938B1 WO2004011938B1 (en) 2004-09-16

Family

ID=30011408

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/FR2003/002298 WO2004011938A2 (en) 2002-07-23 2003-07-21 Method and device for screening molecules in cells

Country Status (6)

Country Link
EP (1) EP1525472A2 (en)
JP (1) JP2005533509A (en)
AU (1) AU2003269041A1 (en)
CA (1) CA2492933A1 (en)
FR (1) FR2842747B1 (en)
WO (1) WO2004011938A2 (en)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2857451B1 (en) * 2003-07-11 2005-09-30 Commissariat Energie Atomique Method and device for analysis of live reaction environments
WO2005118129A1 (en) * 2004-05-27 2005-12-15 Stratos Biosystems, Llc Solid-phase affinity-based method for preparing and manipulating an analyte-containing solution
ES2390800T3 (en) 2005-01-28 2012-11-16 Duke University Apparatus and methods for handling droplets on a printed circuit board
WO2006106748A1 (en) * 2005-03-30 2006-10-12 National University Corporation Nagoya University Method for production of biological organic material and culture vessel
JPWO2007063898A1 (en) * 2005-11-29 2009-05-07 国立大学法人 東京大学 Protein crystallization method and apparatus, and protein crystallization treatment apparatus
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US9476856B2 (en) 2006-04-13 2016-10-25 Advanced Liquid Logic, Inc. Droplet-based affinity assays
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
KR101503510B1 (en) 2007-02-09 2015-03-18 어드밴스드 리퀴드 로직, 아이엔씨. Droplet actuator devices and methods employing magnetic beads
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
CN101945767B (en) 2007-12-23 2013-10-30 先进液体逻辑公司 Droplet actuator configurations and methods of conducting droplet operations
WO2009137415A2 (en) 2008-05-03 2009-11-12 Advanced Liquid Logic, Inc. Reagent and sample preparation, loading, and storage
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011057197A2 (en) 2009-11-06 2011-05-12 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel electrophoresis and molecular analysis
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
WO2011142117A1 (en) 2010-05-11 2011-11-17 パナソニック株式会社 Cell culture substrate and cell culture method using same
CA2833897A1 (en) 2011-05-09 2012-11-15 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
EP2729792A4 (en) 2011-07-06 2015-03-18 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
EP2867645B1 (en) 2012-06-27 2019-06-05 Advanced Liquid Logic, Inc. Techniques and droplet actuator designs for reducing bubble formation
WO2014165273A1 (en) 2013-03-13 2014-10-09 Innovative Surface Technologies, Inc. Conical devices for three-dimensional aggregate (s) of eukaryotic cells
GB201518392D0 (en) * 2015-10-16 2015-12-02 Isis Innovation Microfluidic arrangements

Citations (3)

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WO1997039328A1 (en) * 1996-04-12 1997-10-23 Australian Biomedical Corporation Ltd. Method and apparatus for treatment of human or animal cell samples
WO1998041869A1 (en) * 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
WO1999055827A1 (en) * 1998-04-27 1999-11-04 Amersham Pharmacia Biotech Uk Ltd. Microfabricated apparatus for cell based assays

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997039328A1 (en) * 1996-04-12 1997-10-23 Australian Biomedical Corporation Ltd. Method and apparatus for treatment of human or animal cell samples
WO1998041869A1 (en) * 1997-03-18 1998-09-24 Chromaxome Corporation Methods for screening compounds using encapsulated cells
WO1999055827A1 (en) * 1998-04-27 1999-11-04 Amersham Pharmacia Biotech Uk Ltd. Microfabricated apparatus for cell based assays

Non-Patent Citations (2)

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Title
"Toxicity bioassay of heavy metals in water using Tetrahymena pyriformis", WATER RESEARCH 1973, vol. 7, no. 7, 1973, pages 951 - 961, XP008026543 *
ZIAUDDIN JUNAID ET AL: "Microarrays of cells expressing defined cDNAs.", NATURE (LONDON), vol. 411, no. 6833, 2001, pages 107 - 110, XP002246363, ISSN: 0028-0836 *

Also Published As

Publication number Publication date
WO2004011938B1 (en) 2004-09-16
FR2842747B1 (en) 2004-10-15
FR2842747A1 (en) 2004-01-30
WO2004011938A2 (en) 2004-02-05
CA2492933A1 (en) 2004-02-05
AU2003269041A1 (en) 2004-02-16
JP2005533509A (en) 2005-11-10
AU2003269041A8 (en) 2004-02-16
EP1525472A2 (en) 2005-04-27

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