WO2003088917A2 - Regulation of tnf-alpha - Google Patents

Regulation of tnf-alpha Download PDF

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Publication number
WO2003088917A2
WO2003088917A2 PCT/US2003/012262 US0312262W WO03088917A2 WO 2003088917 A2 WO2003088917 A2 WO 2003088917A2 US 0312262 W US0312262 W US 0312262W WO 03088917 A2 WO03088917 A2 WO 03088917A2
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WO
WIPO (PCT)
Prior art keywords
tnf
alpha
compound
compounds
nmr
Prior art date
Application number
PCT/US2003/012262
Other languages
French (fr)
Other versions
WO2003088917A3 (en
Inventor
Karen Miller
Anita Diu-Hercend
Thierry Hercend
Paul Lang
Peter Weber
Julian Golec
Michael Mortimore
Original Assignee
Vertex Pharmaceuticals Incorporated
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Publication date
Application filed by Vertex Pharmaceuticals Incorporated filed Critical Vertex Pharmaceuticals Incorporated
Priority to EP03721795A priority Critical patent/EP1499898A2/en
Priority to AU2003225088A priority patent/AU2003225088A1/en
Publication of WO2003088917A2 publication Critical patent/WO2003088917A2/en
Publication of WO2003088917A3 publication Critical patent/WO2003088917A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D235/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, condensed with other rings
    • C07D235/02—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, condensed with other rings condensed with carbocyclic rings or ring systems
    • C07D235/04—Benzimidazoles; Hydrogenated benzimidazoles
    • C07D235/24—Benzimidazoles; Hydrogenated benzimidazoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached in position 2
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/64—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings
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    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/64—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings
    • C07C233/81—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C07C233/82—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom
    • C07C233/83—Carboxylic acid amides having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom of an acyclic saturated carbon skeleton
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    • C07C235/44—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring
    • C07C235/52—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to carbon atoms of six-membered aromatic rings and singly-bound oxygen atoms bound to the same carbon skeleton with carbon atoms of carboxamide groups and singly-bound oxygen atoms bound to carbon atoms of the same non-condensed six-membered aromatic ring having the nitrogen atom of at least one of the carboxamide groups bound to an acyclic carbon atom of a hydrocarbon radical substituted by carboxyl groups
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    • C07C237/22—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atoms of the carboxamide groups bound to acyclic carbon atoms of the carbon skeleton having nitrogen atoms of amino groups bound to the carbon skeleton of the acid part, further acylated
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    • C07C237/28—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton
    • C07C237/42—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by amino groups having the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a non-condensed six-membered aromatic ring of the carbon skeleton having nitrogen atoms of amino groups bound to the carbon skeleton of the acid part, further acylated
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    • C07C271/08—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms
    • C07C271/10—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C271/22—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atoms of the carbamate groups bound to hydrogen atoms or to acyclic carbon atoms to carbon atoms of hydrocarbon radicals substituted by carboxyl groups
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    • C07C271/26—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring
    • C07C271/28—Esters of carbamic acids having oxygen atoms of carbamate groups bound to acyclic carbon atoms with the nitrogen atom of at least one of the carbamate groups bound to a carbon atom of a six-membered aromatic ring to a carbon atom of a non-condensed six-membered aromatic ring
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    • C07D209/04—Indoles; Hydrogenated indoles
    • C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
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    • C07D209/56—Ring systems containing three or more rings
    • C07D209/80—[b, c]- or [b, d]-condensed
    • C07D209/82—Carbazoles; Hydrogenated carbazoles
    • C07D209/86—Carbazoles; Hydrogenated carbazoles with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to carbon atoms of the ring system
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    • C07D211/00—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings
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    • C07D211/06—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members
    • C07D211/36—Heterocyclic compounds containing hydrogenated pyridine rings, not condensed with other rings with only hydrogen or carbon atoms directly attached to the ring nitrogen atom having no double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D211/60—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D217/00—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems
    • C07D217/22—Heterocyclic compounds containing isoquinoline or hydrogenated isoquinoline ring systems with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to carbon atoms of the nitrogen-containing ring
    • C07D217/24—Oxygen atoms
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    • C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
    • C07D233/66—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
    • C07D233/90—Carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals
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    • C07D239/00—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings
    • C07D239/70—Heterocyclic compounds containing 1,3-diazine or hydrogenated 1,3-diazine rings condensed with carbocyclic rings or ring systems
    • C07D239/72—Quinazolines; Hydrogenated quinazolines
    • C07D239/86—Quinazolines; Hydrogenated quinazolines with hetero atoms directly attached in position 4
    • C07D239/88—Oxygen atoms
    • C07D239/90—Oxygen atoms with acyclic radicals attached in position 2 or 3
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    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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    • C07D405/12—Heterocyclic compounds containing both one or more hetero rings having oxygen atoms as the only ring hetero atoms, and one or more rings having nitrogen as the only ring hetero atom containing two hetero rings linked by a chain containing hetero atoms as chain links
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    • C07D417/06—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
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    • C07D455/00—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
    • C07D455/03—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine
    • C07D455/04—Heterocyclic compounds containing quinolizine ring systems, e.g. emetine alkaloids, protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing quinolizine ring systems directly condensed with at least one six-membered carbocyclic ring, e.g. protoberberine; Alkylenedioxy derivatives of dibenzo [a, g] quinolizines, e.g. berberine containing a quinolizine ring system condensed with only one six-membered carbocyclic ring, e.g. julolidine
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    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • C—CHEMISTRY; METALLURGY
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    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/30—Phosphinic acids [R2P(=O)(OH)]; Thiophosphinic acids ; [R2P(=X1)(X2H) (X1, X2 are each independently O, S or Se)]
    • C07F9/32—Esters thereof
    • C07F9/3258—Esters thereof the ester moiety containing a substituent or a structure which is considered as characteristic
    • C07F9/3264—Esters with hydroxyalkyl compounds
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    • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
    • C07F9/02—Phosphorus compounds
    • C07F9/547—Heterocyclic compounds, e.g. containing phosphorus as a ring hetero atom
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    • C07F9/6509—Six-membered rings
    • C07F9/6512—Six-membered rings having the nitrogen atoms in positions 1 and 3
    • C07F9/65128—Six-membered rings having the nitrogen atoms in positions 1 and 3 condensed with carbocyclic rings or carbocyclic ring systems
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    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/02—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link
    • C07K5/0202—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing at least one abnormal peptide link containing the structure -NH-X-X-C(=0)-, X being an optionally substituted carbon atom or a heteroatom, e.g. beta-amino acids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/52—Assays involving cytokines
    • G01N2333/525—Tumor necrosis factor [TNF]
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    • G01N2500/00—Screening for compounds of potential therapeutic value
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Definitions

  • This invention relates to compounds and pharmaceutical compositions that regulate TNF-alpha levels and activity and methods for using them.
  • the invention also relates to kits comprising a compound or pharmaceutical composition of this invention and a tool for measuring TNF-alpha levels and/or activity.
  • tumor necrosis factor refers to two closely related cytokines (encoded by separate genes) known as tumor necrosis factor-alpha (TNF, cachectin) and tumor necrosis factor-beta (lymphotoxin, TNF-beta) . Both cytokines interact with the same cell membrane receptors, and both have been implicated as pathogenic mediators of human illness.
  • TNF-alpha participates in the signaling pathways that regulate cell apoptosis and inflammation.
  • TNF-alpha is also known as TNFSF2 , TNFA and DIF.
  • TNF- alpha is a pro-inflammatory mammalian protein capable of inducing cellular effects by virtue of its interaction with specific cellular receptors. It is produced primarily by activated monocytes and macrophages.
  • Lipopoly-sacccharide (LPS, also called endotoxin) derived from the cell wall of gram negative bacteria, is a potent stimulator of TNF-alpha synthesis .
  • TNF-alpha Due to the deleterious effects which can result from an over-production or an unregulated production of TNF-alpha, considerable efforts have been made to regulate the tissue or serum level of TNF- alpha.
  • the pathology of a number of diseases are affected by TNF-alpha, including, restinosis, inflammatory diseases of the central nervous system, demyelinating diseases of the nervous system, multiple sclerosis, septic arthritis, aneurysmal aortic disease, traumatic joint injury, periodontal disease, macular degeneration, diabetic retinopathy, occular inflammation, keratoconus, Sjogren's syndrome, corneal graft rejection, cachexia, and anorexia.
  • TNF-alpha inhibitors While a number of inhibitors of TNF-alpha levels and activity have been reported, it is not clear whether they possess the appropriate pharmacological properties to be therapeutically useful. Therefore, there is a continued need for small molecule TNF-alpha inhibitors that are potent, stable, and have good penetration through membranes to provide effective inhibition of apoptosis in vivo . Such compounds would be extremely useful in treating the aforementioned disease states where TNF-alpha cytokines play a role. Summary of the Invention
  • the present invention relates to compounds that are potent inhibitors of TNF activity.
  • these compounds are expected to have improved cell penetration and pharmacokinetic properties and, as a consequence of their potency, have improved efficacy against diseases where caspases and/or TNF-alpha are implicated.
  • the invention also relates to methods for inhibiting the release of TNF-alpha from various cells and decreasing TNF-alpha levels or activity using the compounds and compositions of this invention.
  • the invention also relates to methods for identifying agents useful for decreasing TNF-alpha levels or activity and treating TNF-alpha mediated diseases.
  • the invention additionally relates to kits comprising the compounds and compositions disclosed herein and a tool for measuring TNF-alpha levels or activity.
  • FIG. 1 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/55114.
  • FIG. 2 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/55127.
  • FIG. 3 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/61542.
  • FIG. 4 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO
  • FIG. 5 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/10383.
  • FIG. 6 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/16093.
  • FIG. 7 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/42216.
  • FIG. 8 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/72707.
  • FIG. 9 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/90070.
  • FIG. 10 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/94351.
  • FIG. 11 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 60/292,969 and PCT Publication WO 02/094263.
  • FIG. 12 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 10/012,722 and PCT Publication WO 02/42278
  • FIG. 13 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,184,210
  • FIG. 14 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,184,244
  • FIG. 15 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,187,771
  • FIG. 16 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,197,750
  • FIG. 17 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,242,422
  • FIG. 18 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions were also described at the April 2001 American Chemical Society (ACS) meeting in San Diego, California, USA.
  • FIG. 19 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 02/22611 and in US Patent Application Publication US2002/0058630.
  • FIG. 20 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 10/127324. Detailed Description of the Invention This invention provides compounds disclosed herein and pharmaceutically acceptable derivatives thereof that are particularly effective as regulators of TNF alpha levels and/or activity. The compounds can be useful to treat TNF-alpha mediated disease states in mammals. The compounds of this invention are recited in FIGs. 1-20.
  • the compounds of this invention inhibit TNF- alpha activity and/or decrease TNF-alpha levels. These compounds can be assayed, for example, for their ability to inhibit the release of TNF-alpha, and/or regulate TNF-alpha levels and/or TNF-alpha activity. Assays for each of the activities are known in the art, including those described below in detail in the
  • these compounds are capable of targeting and inhibiting events in TNF-alpha mediated diseases, and the ultimate activity of the relevant protein in a number of diseases, such as inflammatory diseases, autoimmune diseases, destructive bone, proliferative disorders, infectious diseases, and degenerative diseases.
  • Compounds of this invention also inhibit the release of TNF-alpha from activated cells.
  • cells activated to produce TNF-alpha due to the presence of Hpopolysaccharide, bacteria and/or virus will release less TNF-alpha after exposure to the compounds of this invention.
  • compositions and methods of this invention will be useful for controlling TNF-alpha levels and/or activity in vitro or in vivo .
  • compositions and methods of this invention will thus be useful for controlling TNF-alpha levels in vivo and for treating or reducing the advancement, severity or effects of TNF-alpha mediated conditions, including diseases, disorders or effects.
  • the invention provides a composition
  • a composition comprising a compound of this invention or a pharmaceutically acceptable derivative (e.g., salt) thereof, as described above, and a pharmaceutically acceptable carrier.
  • compositions of this invention may further comprise another therapeutic agent.
  • agents include, but are not limited to, a thrombolytic agent such as tissue plasminogen activator and streptokinase, an anti- inflammatory agent, a matrix metalloprotease inhibitor, a lipoxygenase inhibitor, a cytokine antagonist, an immunosuppressant , an anti-cancer agent, an anti-viral agent, a cytokine, a growth factor, an immunomodulator (e.g., bropirimine, anti-human alpha interferon antibody, IL-2, GM-CSF, methionine enkephalin, interferon alpha, diethyldithiocarbamate, tumor necrosis factor, naltrexone and rEPO) , a prostaglandin, or an anti-vascular hyperproliferation compound.
  • a thrombolytic agent such as tissue plasminogen activator and streptokinase
  • an anti-inflammatory agent such as tissue
  • pharmaceutically acceptable carrier refers to a non-toxic carrier that may be administered to a patient, together with a compound of this invention, and which does not destroy the pharmacological activity thereof.
  • compositions include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat .
  • ion exchangers alumina, aluminum stearate, lecithin
  • serum proteins such as human serum albumin
  • buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial gly
  • compositions comprising only a compound of this invention as the active component
  • methods for administering these compositions may additionally comprise the step of administering to the subject an additional agent.
  • agents include, but are not limited to, a thrombolytic agent such as tissue plasminogen activator and streptokinase, an anti -inflammatory agent, a matrix metalloprotease inhibitor, a lipoxygenase inhibitor, a cytokine antagonist, an immunosuppressant , an anti-cancer agent, an anti-viral agent, a cytokine, a growth factor, an immunomodulator (e.g., bropirimine, anti-human alpha interferon antibody, IL-2, GM-CSF, methionine enkephalin, interferon alpha, diethyldithiocarbamate, tumor necrosis factor, naltrexone and rEPO) , a prostaglandin, or an anti-vascular hyperproliferation compound.
  • a thrombolytic agent such
  • the second agent When a second agent is used, the second agent may be administered either as a separate dosage form or as part of a single dosage form with the compounds or compositions of this invention.
  • the amount of compound present in the above- described compositions should be sufficient to cause a detectable decrease in the severity of the disease, or in TNF-alpha levels or activity. If pharmaceutically acceptable salts of the compounds of this invention are utilized in these compositions, those salts are preferably derived from inorganic or organic acids and bases.
  • acid salts include the following: acetate, adipate, alginate, aspartate, benzoate, benzene sulfonate, bisulfate, butyrate, citrate, ca phorate, camphor sulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3- phenyl-propionate, picrate, pivalate, propionate, succinate, tartrate,
  • Base salts include ammonium salts, alkali metal salts, such as sodium and potassium salts, alkaline earth metal salts, such as calcium and magnesium salts, salts with organic bases, such as dicyclohexylamine salts, N-methyl-D-glucamine, and salts with amino acids such as arginine, lysine, and so forth.
  • the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides; dialkyl sulfates, such as dimethyl, diethyl, dibutyl and diamyl sulfates; long chain halides such as decyl , lauryl, myristyl and stearyl chlorides, bromides and iodides; aralkyl halides, such as benzyl and phenethyl bromides and others. Water or oil-soluble or dispersible products are thereby obtained.
  • lower alkyl halides such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides
  • dialkyl sulfates such as dimethyl, diethyl, dibutyl and diamyl sulfates
  • long chain halides
  • compositions and methods of this invention may also be modified by appending appropriate functionalities to enhance selective biological properties.
  • modifications are known in the art and include those which increase biological penetration into a given biological system (e.g., blood, lymphatic system, or central nervous system) , increase oral availability, increase solubility to allow administration by injection, alter metabolism and/or alter rate of excretion.
  • compositions of this invention are formulated for pharmaceutical administration to a subject, e.g., a mammal, preferably a human being.
  • Such pharmaceutical compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir.
  • parenteral as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection and infusion techniques.
  • the compositions are administered orally or intravenously.
  • Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
  • the sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example as a solution in 1 , 3 -butanediol .
  • a nontoxic parenterally acceptable diluent or solvent for example as a solution in 1 , 3 -butanediol .
  • acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution.
  • sterile, fixed oils are conventionally employed as a solvent or suspending medium.
  • any bland fixed oil may be employed including synthetic mono-or di-glycerides .
  • Fatty acids such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil and castor oil, especially in their polyoxyethylated versions .
  • These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
  • a long-chain alcohol diluent or dispersant such as carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions.
  • Other commonly used surfactants such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
  • the preparation can be tableted, placed in a hard gelatin capsule in powder or pellet form, or in the form of a troche or lozenge.
  • the amount of solid carrier will vary, e.g., from about 25 mg to 400 mg.
  • the preparation can be, e.g., in the form of a syrup, emulsion, soft gelatin capsule, sterile injectable liquid such as an ampule or nonaqueous liquid suspension.
  • any routine encapsulation is suitable, for example, using the aforementioned carriers in a hard gelatin capsule shell .
  • a syrup formulation can consist of a suspension or solution of the compound in a liquid carrier for example, ethanol, glycerin, or water with a flavoring or coloring agent.
  • An aerosol preparation can consist of a solution or suspension of the compound in a liquid carrier such as water, ethanol or glycerin; whereas in a powder dry aerosol, the preparation can include e.g., a wetting agent.
  • Formulations of the present invention comprise an active ingredient together with one or more acceptable carrier (s) thereof and optionally any other therapeutic ingredient (s) .
  • the carrier (s) should be "acceptable” in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof .
  • compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, and aqueous suspensions or solutions.
  • carriers that are commonly used include lactose and corn starch.
  • Lubricating agents such as magnesium stearate, are also typically added.
  • useful diluents include lactose and dried cornstarch.
  • aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
  • compositions of this invention may be administered in the form of suppositories for rectal administration.
  • suppositories for rectal administration.
  • suppositories can be prepared by mixing the agent with a suitable non- irritating excipient which is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug.
  • suitable non- irritating excipient include cocoa butter, beeswax and polyethylene glycols.
  • compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs. Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used.
  • the pharmaceutical compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers.
  • Carriers for topical administration of the compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water.
  • the pharmaceutical compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers.
  • Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2- octyldodecanol , benzyl alcohol and water.
  • the pharmaceutical compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride.
  • the pharmaceutical compositions may be formulated in an ointment such as petrolatum.
  • compositions of this invention may also be administered by nasal aerosol or inhalation.
  • Such compositions are prepared according to techniques well known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents known in the art.
  • the form and character of the pharmaceutically acceptable carrier or diluent is dictated by the amount of active ingredient with which it is to be combined, the route of administration, and other well-known variables .
  • TNF-alpha mediated disease means, all diseases states in which TNF-alpha plays a role, either by excessive production or release of TNF-alpha itself, or by TNF-alpha causing an event such as production or release of another pathophysiological biochemical agent or cytokine. In one preferred embodiment, TNF-alpha plays a direct role in the disease.
  • Such diseases can include, e.g., restinosis, inflammatory diseases such as inflammatory diseases of the central nervous system, demyelinating diseases of the nervous system, multiple sclerosis, septic arthritis, aneurysmal aortic disease, traumatic joint injury, peridontal disease, macular degeneration, diabetic retinopathy, occular inflammation, keratoconus, Sjogren's syndrome, corneal graft rejection, cachexia, and anorexia.
  • inflammatory diseases such as inflammatory diseases of the central nervous system, demyelinating diseases of the nervous system, multiple sclerosis, septic arthritis, aneurysmal aortic disease, traumatic joint injury, peridontal disease, macular degeneration, diabetic retinopathy, occular inflammation, keratoconus, Sjogren's syndrome, corneal graft rejection, cachexia, and anorexia.
  • TNF-alpha tissue levels have been implicated in mediating or exacerbating a number of diseases including: rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, gouty arthritis and other arthritic conditions, general sepsis, gram-negative sepsis, septic shock, endotoxic shock, toxic shock syndrome, adult respiratory distress syndrome (ARDS) , cerebral malaria, chronic pulmonary inflammatory disease, silicosis, asbestosis, pulmonary sarcoidosis, bone resorption diseases, graft vs.
  • autoimmune diseases multiple sclerosis, autoimmune diabetes, and systemic lupus erythematosus .
  • TNF-alpha inhibitors are useful in the treatment of a variety of allergic, traumatic and other injurious disorders including: asthma, chronic bronchitis, atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, eosiniophilic granuloma, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, and adult respiratory distress syndrome (ARDS) .
  • asthma chronic bronchitis
  • atopic dermatitis urticaria
  • allergic rhinitis allergic conjunctivitis
  • eosiniophilic granuloma ulcerative colitis
  • Crohn's disease reperfusion injury of the myocardium and brain
  • ARDS adult respiratory distress syndrome
  • the compounds of this invention can inhibit the release of TNF-alpha and thus can be useful for inhibiting or blocking several pathophysiological effects of TNF-alpha at injury or surgery sites and thus also inhibit the release of other pathophysiological biochemical products from cells such as histamines, prostaglandins , bradykinins, and peroxidases .
  • TNF-alpha inhibitors can be very effective in the treatment of disorders which follow cellular, tissue or organ injury or surgery, and can be as effective, or even more potent, than corticosteroids or immunosuppressants without producing the side effects common to these agents .
  • This invention also relates to a therapeutic method of (1) inhibiting TNF-alpha release from cells and/or (2) preventing the untoward, toxic or lethal effects of excessively high tissue levels of TNF-alpha in a mammal, including a human. This method comprises administering to a mammal an effective TNF-alpha inhibiting quantity of one or more of the above compounds.
  • This method also can be used for the prophylactic treatment or prevention of certain TNF- alpha mediated or exacerbated diseases amenable thereto.
  • the invention provides a method for the treatment of allergic, traumatic, radiation, chemical, microbial and other injurious disorders by administering to a mammal, including a human, in need thereof an effective amount of such compounds.
  • the compounds by inhibiting or blocking the release of TNF-alpha or decreasing TNF-alpha levels and activity, as well as the pathophysiologic actions of excessive levels of TNF-alpha in each of these circumstances, directly facilitate the arrest or resolution of the tissue or organ damage, and facilitates the restoration of normal function. Together, these actions relate their novel use in treating tissue trauma, or other injury disorders caused by infection, allergy, immunologic phenomena, burns, radiation exposure, neoplastic disease, toxic chemicals and expressed as cardiovascular damage, neurologic injury, renal damage, liver damage, pancreatic damage, as well as ascites, localized edema, dermal damage and dermal blister.
  • inhibiting the release of TNF- alpha means: a) decrease of in vivo TNF-alpha levels in a mammal such as a human; b) a down regulation of TNF-alpha levels in vitro or in-vivo; or c) a down regulation of TNF-alpha activity, by inhibition of the direct synthesis of TNF-alpha or a post-translation event in vivo or in vitro.
  • the compounds can be useful in inhibiting the release of TNF-alpha by monocytes, macrophages, neuronal cells, endothelial cells, epidermal cells, mesenchymal cells (for example: fibroblasts, skeletal myocytes, smooth muscle myocytes, cardiac myocytes) and many other types of cells.
  • condition or "state” refers to any disease, disorder or effect that produces deleterious biological consequences in a subject.
  • the level of TNF-alpha protein in the blood or cell of a patient or a cell culture can be determined by for example, assaying for immunospecific binding to TNF-alpha or to other proteins known to be produced as a result of the presence of active TNF-alpha. Such methods are known in the art.
  • immunoassays which can be used include, but are not limited to competitive and non-competitive assay systems, western blots, radioimmunoassays, ELISA (enzyme linked immunosorbent assay) , "sandwich” immunoassays, immunoprecipitation assays, precipitin reactions, gel diffusion precipitin reactions, immunodiffusion assays, agglutination assays, complement-fixation assays, immunoradiometric assays, fluorescent immunoassays, protein A immunoassays and FACS analysis with labeled antibodies.
  • competitive and non-competitive assay systems include, but are not limited to competitive and non-competitive assay systems, western blots, radioimmunoassays, ELISA (enzyme linked immunosorbent assay) , "sandwich” immunoassays, immunoprecipitation assays, precipitin reactions, gel diffusion precipitin reactions, immunodiffusion assays, agglutin
  • a competitive binding assay is a radioimmunoassay comprising the incubation of labeled proteins from cells expressing TNF-alpha (e.g., 3 H or 125 I) with a TNF-alpha antibody in the presence of increasing amounts of unlabeled TNF-alpha, and the detection of the TNF-alpha antibody bound to the labeled TNF-alpha.
  • TNF-alpha e.g., 3 H or 125 I
  • the binding off-rates can be determined from the data by Scatchard plot analysis .
  • Competition with a second antibody can also be determined using radioimmunoassays.
  • the antigen is incubated with antibody of interest conjugated to a labeled compound (e.g., 3 H or 125 I) in the presence of increasing amounts of an unlabeled second antibody.
  • a labeled compound e.g., 3 H or 125 I
  • TNF-alpha levels can also be assayed by activity, for example, TNF-alpha levels can be assayed by a cell line that is capable of detecting bioactive levels of cytokines like TNF-alpha or a growth factor.
  • the levels of bioactive TNF-alpha in a biological sample is detected by incubating a cell line genetically engineered with isopropyl-b-D-thiogalactopyranoside . The cell line is incubated with the sample to be tested and cell death in the cell line is monitored by determining the intensity of blue color which is indicative of a bioactive cytokine or growth factor in the sample tested. See also, e.g., Burns (1994) 20(l):40-44 for TNF activity assay of serum of patients.
  • a cytotoxicity assay can be used to e.g., determine TNF-alpha activity with actinomycin D-treated ME180 cells and L929 cells can be used in an assay described by Ostrove and Gifford (Proc. Soc . Ex . Biol. Med. 160, 354-358 (1979)), Aggarwal and Essalu (J. Biol. Chem. 262, 10000-10007 (1987)) and Levesque et al. (J. Immun. Meth. 178, 71-76 (1995)).
  • L929 cells (CCLI: American Type Culture Collection) are maintained in McCoy's 5A medium containing 10% fetal bovine serum.
  • Confluent cultures were treated briefly with 0.25% trypsin in physiological solution and resuspended in a fresh medium.
  • the trypsinized cells per well are plated in 96-Well plates (Corning) and incubated for 24 hours at 37°C.
  • actinomycin D was added to a final concentration of 0.25 ug/ml .
  • Samples containing the compounds to be tested are added to the wells and incubation is continued overnight at the same temperature. After microscopic evaluation, the medium is decanted, and the wells are rinsed with PBS. The wells are then filled with a crystal violet dye solution. The dye is extracted and the absorbance of the dye retained in viable cells is determined at 570 nm.
  • Dosage levels of between about 0.01 and about 100 mg/kg body weight per day, preferably between about 0.5 and about 75 mg/kg body weight per day and most preferably between about 1 and about 50 mg/kg body weight per day of the active ingredient compound are useful in a monotherapy.
  • the pharmaceutical compositions of this invention will be administered from about 1 to 5 times per day or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy.
  • the amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration.
  • a typical preparation will contain from about 5% to about 95% active compound (w/w) .
  • such preparations contain from about 20% to about 80% active compound .
  • both the compound and the additional agent should be present at dosage levels of between about 10% to about 80% of the dosage normally administered in a monotherapy regime.
  • a maintenance dose of a compound, composition or combination of this invention may be administered, if necessary.
  • the dosage or frequency of administration, or both may be reduced, as a function of the symptoms, to a level at which the improved condition is retained.
  • treatment should cease. Patients may, however, require intermittent treatment on a long-term basis upon any recurrence or disease symptoms.
  • a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated.
  • the amount of active ingredients will also depend upon the particular compound and other therapeutic agent, if present, in the composition.
  • a method for treating or preventing a disease of this invention in a subject comprises the step of administering to the subject any compound, pharmaceutical composition, or combination described herein.
  • the invention provides a method of treating a mammal, having one of the aforementioned diseases, comprising the step of administering to said mammal a pharmaceutically acceptable composition described above.
  • a pharmaceutically acceptable composition described above.
  • the patient if the patient is also administered another therapeutic agent, it may be delivered together with the compound of this invention in a single dosage form, or, as a separate dosage form.
  • the other therapeutic agent may be administered prior to, at the same time as, or following administration of a pharmaceutically acceptable composition comprising a compound of this invention.
  • a kit according to this invention comprises a compound or pharmaceutical composition of this invention and a tool for measuring TNF-alpha levels or activity in vitro or in vivo .
  • the kit can further comprise instructions for using the contents of the kit.
  • a tool for measuring TNF-alpha levels or activity according to this invention refers to materials that can be used to measure the TNF gene product (i.e., RNA or protein) or activity. Such methods are described for example above.
  • a tool according to this invention can include, e.g., an anti-TNF antibody, a TNF-alpha DNA probe or a genetically engineered cell line responsive to TNF-alpha levels described above.
  • the methods for identifying a compound or composition that decreases TNF-alpha activity and/or levels according to this invention include methods for screening of a plurality of compounds or compositions for their ability to decrease TNF-alpha activity and/or levels.
  • high-throughput screening is a desired embodiment of this invention.
  • high throughput screening can be achieved by having cells in culture in a plurality of wells in a microtiter plate, adding a different compound or composition to each well and comparing the TNF-alpha levels and/or activity in each cell culture to the TNF-alpha levels or activity present in a cell culture in a control well.
  • Controls that are useful for the comparison step according to this invention include cells or subjects that have not been treated with a compound or composition and cells or subjects have been treated with a compound or composition that is known to have no effect on TNF- alpha levels or activity.
  • the high throughput screening is automated so that the steps including the addition of the cells to the plate up to the data collection and analysis after addition of the compound or composition are done by machine .
  • Instruments that are useful in the comparison step of this invention e.g., instruments that can detect labeled objects (e.g., radiolabelled, fluorescent or colored objects) or objects that are themselves detectable, are commercially available and/or known in the art . Accordingly, compounds and compositions according to this invention that are useful for decreasing TNF-alpha levels and/or activity can be quickly and efficiently screened.
  • Trifluoroacetic acid (5mL) was added to a stirred ice cold solution of [3S/R, (2S) ] -5-fluoro-4- oxo-3- ⁇ [1- (phenothiazine-10-carbonyl) piperidine-2- carbonyl] amino ⁇ -pentanoic acid tert-butyl ester (130mg, 0.24mmol) in anhydrous DCM (5mL) .
  • the mixture was stirred at 0°C for 0.5h then at room temperature for 0.5h.
  • the mixture was concentrated under reduced pressure and then the residue was dissolved in dry DCM. This process was repeated several times in order to remove excess trifluoroacetic acid.
  • the gum was lyophilized twice from HPLC grade water to afford the title compound as a white powder (77mg, 66%) -.
  • reaction mixture was then allowed to stir for a further 3.5h at -78 °C after which it was diluted with NH 4 C1 solution, diluted with ethyl acetate and washed sequentially with NaHC0 3 solution and brine.
  • the organic phase was dried (Na 2 S0 4 ) and concentrated to give a gum.
  • reaction mixture was warmed to ambient temperature over 15h after which it was diluted with NH 4 C1 solution and ethyl acetate and washed with brine.
  • the organic phase was dried (Na 2 S0 4 ) and concentrated to give a gum.
  • the resulting mixture was kept at 0°C for 3h, diluted with DCM, and then washed sequentially with saturated aqueous sodium thiosulphate, NaHC0 3 solution and brine. The organics were dried (Na 2 S0 ) and concentrated.
  • Example 15 Inhibition of TNF release from whole blood
  • Human blood was freshly drawn from healthy donors and collected in vacutainers . Blood was diluted 1 : 2 in PBS (tissue culture, pyrogen free) in a sterile bottle and inverted to mix well. Aliquots of 0.5 ml of blood mixture were dispensed into cluster tubes in 96 well format.
  • PBS tissue culture, pyrogen free
  • Dilutions of the test compounds were prepared in RPMI by taking lOOmM DMSO stocks of the compounds and diluting 1:10 in RPMI medium in eppendorfs, to give a lOmM stock. 1:5 serial dilutions were prepared from the stock solutions.
  • LPS was kept at a frozen stock (-20 degrees C) at lmg/ml in PBS and then diluted to 1:10 with RPMI medium and finally diluted in the medium again 1:350.
  • 50 ⁇ l of each test compound (first concentration was lOOuM) were added to the blood samples and then stimulated with 10 ⁇ l LPS (final concentration in the well is 5ng/ml) .
  • the . contents were gently mixed using an 8 well multi-channel pipette and incubated at 37°C overnight. At the end of the incubation time, contents were gently mixed, then spun down at 1000 x g for 5 mins at 20°C.
  • TNF-alpha levels of supernatants were assayed using the R+D systems ELISA kit, using R+D systems protocol. Samples were read at 450nm. The compounds shown in Table 1 were tested in the above assay. In this TNF-alpha assay, category "A” indicates an IC50 value of ⁇ 500 nM. Category “B” indicates an IC50 value of 500-1000 nM. Category “C” indicates an IC50 value of 1001-2000 nM. category “D” indicates an IC50 value of > 2000 nM. See Table 1.

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Abstract

The present invention relates to methods for identifying compounds useful for regulating TNF-alpha levels and/or activity. The invention also relates to methods for decreasing TNF-alpha levels and/or activity. Compounds and compositions according to this invention are useful for treating TNF-mediated diseases. The invention also relates to kits comprising the compounds and compositions herein and a tool for measuring TNF-alpha activity and/or levels.

Description

REGULATION OF TNF-ALPHA
Cross-Reference to Related Application This application claims the benefit of United States provisional application No. 60/374,434 filed April 19, 2002 which is hereby incorporated by reference herein in its entirety.
Field of the Invention This invention relates to compounds and pharmaceutical compositions that regulate TNF-alpha levels and activity and methods for using them. The invention also relates to kits comprising a compound or pharmaceutical composition of this invention and a tool for measuring TNF-alpha levels and/or activity.
Background of the Invention
Generally, the term tumor necrosis factor (TNF) refers to two closely related cytokines (encoded by separate genes) known as tumor necrosis factor-alpha (TNF, cachectin) and tumor necrosis factor-beta (lymphotoxin, TNF-beta) . Both cytokines interact with the same cell membrane receptors, and both have been implicated as pathogenic mediators of human illness.
TNF-alpha participates in the signaling pathways that regulate cell apoptosis and inflammation. TNF-alpha is also known as TNFSF2 , TNFA and DIF. TNF- alpha is a pro-inflammatory mammalian protein capable of inducing cellular effects by virtue of its interaction with specific cellular receptors. It is produced primarily by activated monocytes and macrophages. Lipopoly-sacccharide (LPS, also called endotoxin) , derived from the cell wall of gram negative bacteria, is a potent stimulator of TNF-alpha synthesis . Due to the deleterious effects which can result from an over-production or an unregulated production of TNF-alpha, considerable efforts have been made to regulate the tissue or serum level of TNF- alpha. The pathology of a number of diseases are affected by TNF-alpha, including, restinosis, inflammatory diseases of the central nervous system, demyelinating diseases of the nervous system, multiple sclerosis, septic arthritis, aneurysmal aortic disease, traumatic joint injury, periodontal disease, macular degeneration, diabetic retinopathy, occular inflammation, keratoconus, Sjogren's syndrome, corneal graft rejection, cachexia, and anorexia.
While a number of inhibitors of TNF-alpha levels and activity have been reported, it is not clear whether they possess the appropriate pharmacological properties to be therapeutically useful. Therefore, there is a continued need for small molecule TNF-alpha inhibitors that are potent, stable, and have good penetration through membranes to provide effective inhibition of apoptosis in vivo . Such compounds would be extremely useful in treating the aforementioned disease states where TNF-alpha cytokines play a role. Summary of the Invention
The present invention relates to compounds that are potent inhibitors of TNF activity. In addition, these compounds are expected to have improved cell penetration and pharmacokinetic properties and, as a consequence of their potency, have improved efficacy against diseases where caspases and/or TNF-alpha are implicated.
The invention also relates to methods for inhibiting the release of TNF-alpha from various cells and decreasing TNF-alpha levels or activity using the compounds and compositions of this invention. The invention also relates to methods for identifying agents useful for decreasing TNF-alpha levels or activity and treating TNF-alpha mediated diseases. The invention additionally relates to kits comprising the compounds and compositions disclosed herein and a tool for measuring TNF-alpha levels or activity.
Brief Description of the Figures FIG. 1 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/55114.
FIG. 2 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/55127.
FIG. 3 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 00/61542. FIG. 4 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO
Figure imgf000005_0001
FIG. 5 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/10383.
FIG. 6 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/16093.
FIG. 7 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/42216.
FIG. 8 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/72707.
FIG. 9 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/90070. FIG. 10 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 01/94351.
FIG. 11 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 60/292,969 and PCT Publication WO 02/094263. FIG. 12 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 10/012,722 and PCT Publication WO 02/42278 FIG. 13 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,184,210
FIG. 14 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,184,244
FIG. 15 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,187,771
FIG. 16 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,197,750
FIG. 17 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent 6,242,422 FIG. 18 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions were also described at the April 2001 American Chemical Society (ACS) meeting in San Diego, California, USA. FIG. 19 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in PCT Publication WO 02/22611 and in US Patent Application Publication US2002/0058630. FIG. 20 depicts compounds and pharmaceutical compositions of this invention. Said compounds and compositions are also described in US Patent Application 10/127324. Detailed Description of the Invention This invention provides compounds disclosed herein and pharmaceutically acceptable derivatives thereof that are particularly effective as regulators of TNF alpha levels and/or activity. The compounds can be useful to treat TNF-alpha mediated disease states in mammals. The compounds of this invention are recited in FIGs. 1-20.
The compounds of this invention inhibit TNF- alpha activity and/or decrease TNF-alpha levels. These compounds can be assayed, for example, for their ability to inhibit the release of TNF-alpha, and/or regulate TNF-alpha levels and/or TNF-alpha activity. Assays for each of the activities are known in the art, including those described below in detail in the
Examples. Accordingly, these compounds are capable of targeting and inhibiting events in TNF-alpha mediated diseases, and the ultimate activity of the relevant protein in a number of diseases, such as inflammatory diseases, autoimmune diseases, destructive bone, proliferative disorders, infectious diseases, and degenerative diseases.
Compounds of this invention also inhibit the release of TNF-alpha from activated cells. For example, cells activated to produce TNF-alpha due to the presence of Hpopolysaccharide, bacteria and/or virus will release less TNF-alpha after exposure to the compounds of this invention.
The pharmaceutical compositions and methods of this invention, therefore, will be useful for controlling TNF-alpha levels and/or activity in vitro or in vivo . The compositions and methods of this invention will thus be useful for controlling TNF-alpha levels in vivo and for treating or reducing the advancement, severity or effects of TNF-alpha mediated conditions, including diseases, disorders or effects.
According to another embodiment, the invention provides a composition comprising a compound of this invention or a pharmaceutically acceptable derivative (e.g., salt) thereof, as described above, and a pharmaceutically acceptable carrier.
According to another embodiment, the compositions of this invention may further comprise another therapeutic agent. Such agents include, but are not limited to, a thrombolytic agent such as tissue plasminogen activator and streptokinase, an anti- inflammatory agent, a matrix metalloprotease inhibitor, a lipoxygenase inhibitor, a cytokine antagonist, an immunosuppressant , an anti-cancer agent, an anti-viral agent, a cytokine, a growth factor, an immunomodulator (e.g., bropirimine, anti-human alpha interferon antibody, IL-2, GM-CSF, methionine enkephalin, interferon alpha, diethyldithiocarbamate, tumor necrosis factor, naltrexone and rEPO) , a prostaglandin, or an anti-vascular hyperproliferation compound.
The term "pharmaceutically acceptable carrier" refers to a non-toxic carrier that may be administered to a patient, together with a compound of this invention, and which does not destroy the pharmacological activity thereof.
Pharmaceutically acceptable carriers that may be used in these compositions include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, serum proteins such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium carboxymethylcellulose, polyacrylates, waxes, polyethylene-polyoxypropylene-block polymers, polyethylene glycol and wool fat . In pharmaceutical compositions comprising only a compound of this invention as the active component, methods for administering these compositions may additionally comprise the step of administering to the subject an additional agent. Such agents include, but are not limited to, a thrombolytic agent such as tissue plasminogen activator and streptokinase, an anti -inflammatory agent, a matrix metalloprotease inhibitor, a lipoxygenase inhibitor, a cytokine antagonist, an immunosuppressant , an anti-cancer agent, an anti-viral agent, a cytokine, a growth factor, an immunomodulator (e.g., bropirimine, anti-human alpha interferon antibody, IL-2, GM-CSF, methionine enkephalin, interferon alpha, diethyldithiocarbamate, tumor necrosis factor, naltrexone and rEPO) , a prostaglandin, or an anti-vascular hyperproliferation compound. When a second agent is used, the second agent may be administered either as a separate dosage form or as part of a single dosage form with the compounds or compositions of this invention. The amount of compound present in the above- described compositions should be sufficient to cause a detectable decrease in the severity of the disease, or in TNF-alpha levels or activity. If pharmaceutically acceptable salts of the compounds of this invention are utilized in these compositions, those salts are preferably derived from inorganic or organic acids and bases. Included among such acid salts are the following: acetate, adipate, alginate, aspartate, benzoate, benzene sulfonate, bisulfate, butyrate, citrate, ca phorate, camphor sulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, ethanesulfonate, fumarate, glucoheptanoate, glycerophosphate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, methanesulfonate, 2-naphthalenesulfonate, nicotinate, oxalate, pamoate, pectinate, persulfate, 3- phenyl-propionate, picrate, pivalate, propionate, succinate, tartrate, thiocyanate, tosylate and undecanoate. Base salts include ammonium salts, alkali metal salts, such as sodium and potassium salts, alkaline earth metal salts, such as calcium and magnesium salts, salts with organic bases, such as dicyclohexylamine salts, N-methyl-D-glucamine, and salts with amino acids such as arginine, lysine, and so forth.
Also, the basic nitrogen-containing groups can be quaternized with such agents as lower alkyl halides, such as methyl, ethyl, propyl, and butyl chlorides, bromides and iodides; dialkyl sulfates, such as dimethyl, diethyl, dibutyl and diamyl sulfates; long chain halides such as decyl , lauryl, myristyl and stearyl chlorides, bromides and iodides; aralkyl halides, such as benzyl and phenethyl bromides and others. Water or oil-soluble or dispersible products are thereby obtained. The compounds utilized in the compositions and methods of this invention may also be modified by appending appropriate functionalities to enhance selective biological properties. Such modifications are known in the art and include those which increase biological penetration into a given biological system (e.g., blood, lymphatic system, or central nervous system) , increase oral availability, increase solubility to allow administration by injection, alter metabolism and/or alter rate of excretion.
According to a preferred embodiment, the compositions of this invention are formulated for pharmaceutical administration to a subject, e.g., a mammal, preferably a human being. Such pharmaceutical compositions of the present invention may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir. The term "parenteral" as used herein includes subcutaneous, intravenous, intramuscular, intra-articular, intra-synovial, intrasternal, intrathecal, intrahepatic, intralesional and intracranial injection and infusion techniques. Preferably, the compositions are administered orally or intravenously.
Sterile injectable forms of the compositions of this invention may be aqueous or oleaginous suspension. These suspensions may be formulated according to techniques known in the art using suitable dispersing or wetting agents and suspending agents.
The sterile injectable preparation may also be a sterile injectable solution or suspension in a nontoxic parenterally acceptable diluent or solvent, for example as a solution in 1 , 3 -butanediol . Among the acceptable vehicles and solvents that may be employed are water, Ringer's solution and isotonic sodium chloride solution. In addition, sterile, fixed oils are conventionally employed as a solvent or suspending medium. For this purpose, any bland fixed oil may be employed including synthetic mono-or di-glycerides . Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically-acceptable oils, such as olive oil and castor oil, especially in their polyoxyethylated versions . These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, such as carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms including emulsions and suspensions. Other commonly used surfactants, such as Tweens, Spans and other emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of pharmaceutically acceptable solid, liquid, or other dosage forms may also be used for the purposes of formulation.
If a solid carrier is used, the preparation can be tableted, placed in a hard gelatin capsule in powder or pellet form, or in the form of a troche or lozenge. The amount of solid carrier will vary, e.g., from about 25 mg to 400 mg. When a liquid carrier is used, the preparation can be, e.g., in the form of a syrup, emulsion, soft gelatin capsule, sterile injectable liquid such as an ampule or nonaqueous liquid suspension. Where the composition is in the form of a capsule, any routine encapsulation is suitable, for example, using the aforementioned carriers in a hard gelatin capsule shell .
A syrup formulation can consist of a suspension or solution of the compound in a liquid carrier for example, ethanol, glycerin, or water with a flavoring or coloring agent. An aerosol preparation can consist of a solution or suspension of the compound in a liquid carrier such as water, ethanol or glycerin; whereas in a powder dry aerosol, the preparation can include e.g., a wetting agent.
Formulations of the present invention comprise an active ingredient together with one or more acceptable carrier (s) thereof and optionally any other therapeutic ingredient (s) . The carrier (s) should be "acceptable" in the sense of being compatible with the other ingredients of the formulation and not deleterious to the recipient thereof .
The pharmaceutical compositions of this invention may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, and aqueous suspensions or solutions. In the case of tablets for oral use, carriers that are commonly used include lactose and corn starch. Lubricating agents, such as magnesium stearate, are also typically added. For oral administration in a capsule form, useful diluents include lactose and dried cornstarch. When aqueous suspensions are required for oral use, the active ingredient is combined with emulsifying and suspending agents. If desired, certain sweetening, flavoring or coloring agents may also be added.
Alternatively, the pharmaceutical compositions of this invention may be administered in the form of suppositories for rectal administration. These can be prepared by mixing the agent with a suitable non- irritating excipient which is solid at room temperature but liquid at rectal temperature and therefore will melt in the rectum to release the drug. Such materials include cocoa butter, beeswax and polyethylene glycols.
The pharmaceutical compositions of this invention may also be administered topically, especially when the target of treatment includes areas or organs readily accessible by topical application, including diseases of the eye, the skin, or the lower intestinal tract. Suitable topical formulations are readily prepared for each of these areas or organs. Topical application for the lower intestinal tract can be effected in a rectal suppository formulation (see above) or in a suitable enema formulation. Topically-transdermal patches may also be used. For topical applications, the pharmaceutical compositions may be formulated in a suitable ointment containing the active component suspended or dissolved in one or more carriers. Carriers for topical administration of the compounds of this invention include, but are not limited to, mineral oil, liquid petrolatum, white petrolatum, propylene glycol, polyoxyethylene, polyoxypropylene compound, emulsifying wax and water. Alternatively, the pharmaceutical compositions can be formulated in a suitable lotion or cream containing the active components suspended or dissolved in one or more pharmaceutically acceptable carriers. Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2- octyldodecanol , benzyl alcohol and water.
For ophthalmic use, the pharmaceutical compositions may be formulated as micronized suspensions in isotonic, pH adjusted sterile saline, or, preferably, as solutions in isotonic, pH adjusted sterile saline, either with or without a preservative such as benzylalkonium chloride. Alternatively, for ophthalmic uses, the pharmaceutical compositions may be formulated in an ointment such as petrolatum.
The pharmaceutical compositions of this invention may also be administered by nasal aerosol or inhalation. Such compositions are prepared according to techniques well known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other conventional solubilizing or dispersing agents known in the art.
It will be recognized by one of skill in the art that the form and character of the pharmaceutically acceptable carrier or diluent is dictated by the amount of active ingredient with which it is to be combined, the route of administration, and other well-known variables .
The above-described compounds and compositions are also useful in therapeutic applications relating to a TNF mediated disease. The phrase "TNF-alpha mediated disease" means, all diseases states in which TNF-alpha plays a role, either by excessive production or release of TNF-alpha itself, or by TNF-alpha causing an event such as production or release of another pathophysiological biochemical agent or cytokine. In one preferred embodiment, TNF-alpha plays a direct role in the disease.
Such diseases can include, e.g., restinosis, inflammatory diseases such as inflammatory diseases of the central nervous system, demyelinating diseases of the nervous system, multiple sclerosis, septic arthritis, aneurysmal aortic disease, traumatic joint injury, peridontal disease, macular degeneration, diabetic retinopathy, occular inflammation, keratoconus, Sjogren's syndrome, corneal graft rejection, cachexia, and anorexia.
Excessive TNF-alpha tissue levels have been implicated in mediating or exacerbating a number of diseases including: rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, gouty arthritis and other arthritic conditions, general sepsis, gram-negative sepsis, septic shock, endotoxic shock, toxic shock syndrome, adult respiratory distress syndrome (ARDS) , cerebral malaria, chronic pulmonary inflammatory disease, silicosis, asbestosis, pulmonary sarcoidosis, bone resorption diseases, graft vs. host reactions, allograft rejections, fever and myalgias due to bacterial or viral infections, influenza, cachexia secondary to acquired immune deficiency syndrome (AIDS) , keloid formation, scar tissue formation, Crohn's disease, ulcerative colitis, pyresis, a number of "autoimmune diseases", multiple sclerosis, autoimmune diabetes, and systemic lupus erythematosus . TNF-alpha inhibitors are useful in the treatment of a variety of allergic, traumatic and other injurious disorders including: asthma, chronic bronchitis, atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, eosiniophilic granuloma, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, and adult respiratory distress syndrome (ARDS) .
The compounds of this invention can inhibit the release of TNF-alpha and thus can be useful for inhibiting or blocking several pathophysiological effects of TNF-alpha at injury or surgery sites and thus also inhibit the release of other pathophysiological biochemical products from cells such as histamines, prostaglandins , bradykinins, and peroxidases .
As discussed above, TNF-alpha inhibitors can be very effective in the treatment of disorders which follow cellular, tissue or organ injury or surgery, and can be as effective, or even more potent, than corticosteroids or immunosuppressants without producing the side effects common to these agents . This invention also relates to a therapeutic method of (1) inhibiting TNF-alpha release from cells and/or (2) preventing the untoward, toxic or lethal effects of excessively high tissue levels of TNF-alpha in a mammal, including a human. This method comprises administering to a mammal an effective TNF-alpha inhibiting quantity of one or more of the above compounds. This method also can be used for the prophylactic treatment or prevention of certain TNF- alpha mediated or exacerbated diseases amenable thereto. The invention provides a method for the treatment of allergic, traumatic, radiation, chemical, microbial and other injurious disorders by administering to a mammal, including a human, in need thereof an effective amount of such compounds.
The compounds, by inhibiting or blocking the release of TNF-alpha or decreasing TNF-alpha levels and activity, as well as the pathophysiologic actions of excessive levels of TNF-alpha in each of these circumstances, directly facilitate the arrest or resolution of the tissue or organ damage, and facilitates the restoration of normal function. Together, these actions relate their novel use in treating tissue trauma, or other injury disorders caused by infection, allergy, immunologic phenomena, burns, radiation exposure, neoplastic disease, toxic chemicals and expressed as cardiovascular damage, neurologic injury, renal damage, liver damage, pancreatic damage, as well as ascites, localized edema, dermal damage and dermal blister.
The term "inhibiting the release of TNF- alpha" means: a) decrease of in vivo TNF-alpha levels in a mammal such as a human; b) a down regulation of TNF-alpha levels in vitro or in-vivo; or c) a down regulation of TNF-alpha activity, by inhibition of the direct synthesis of TNF-alpha or a post-translation event in vivo or in vitro.
The compounds can be useful in inhibiting the release of TNF-alpha by monocytes, macrophages, neuronal cells, endothelial cells, epidermal cells, mesenchymal cells (for example: fibroblasts, skeletal myocytes, smooth muscle myocytes, cardiac myocytes) and many other types of cells. The term "condition" or "state" refers to any disease, disorder or effect that produces deleterious biological consequences in a subject.
The level of TNF-alpha protein in the blood or cell of a patient or a cell culture (i.e., within the cell or the cell culture media) can be determined by for example, assaying for immunospecific binding to TNF-alpha or to other proteins known to be produced as a result of the presence of active TNF-alpha. Such methods are known in the art. For example, immunoassays which can be used include, but are not limited to competitive and non-competitive assay systems, western blots, radioimmunoassays, ELISA (enzyme linked immunosorbent assay) , "sandwich" immunoassays, immunoprecipitation assays, precipitin reactions, gel diffusion precipitin reactions, immunodiffusion assays, agglutination assays, complement-fixation assays, immunoradiometric assays, fluorescent immunoassays, protein A immunoassays and FACS analysis with labeled antibodies. Such assays well known in the art (see, e.g., Ausubel et al , eds, 1994, Current Protocols in Molecular Biology, Vol. 1, John Wiley & Sons, Inc., New York, which is incorporated by reference herein in its entirety) . Competitive binding assays can also be used to determine the level of TNF-alpha. One example of a competitive binding assay is a radioimmunoassay comprising the incubation of labeled proteins from cells expressing TNF-alpha (e.g., 3H or 125I) with a TNF-alpha antibody in the presence of increasing amounts of unlabeled TNF-alpha, and the detection of the TNF-alpha antibody bound to the labeled TNF-alpha. The affinity of the antibody of interest for a - l y -
particular antigen and the binding off-rates can be determined from the data by Scatchard plot analysis . Competition with a second antibody can also be determined using radioimmunoassays. In this case, the antigen is incubated with antibody of interest conjugated to a labeled compound (e.g., 3H or 125I) in the presence of increasing amounts of an unlabeled second antibody.
TNF-alpha levels can also be assayed by activity, for example, TNF-alpha levels can be assayed by a cell line that is capable of detecting bioactive levels of cytokines like TNF-alpha or a growth factor. According to one embodiment, the levels of bioactive TNF-alpha in a biological sample is detected by incubating a cell line genetically engineered with isopropyl-b-D-thiogalactopyranoside . The cell line is incubated with the sample to be tested and cell death in the cell line is monitored by determining the intensity of blue color which is indicative of a bioactive cytokine or growth factor in the sample tested. See also, e.g., Burns (1994) 20(l):40-44 for TNF activity assay of serum of patients.
A cytotoxicity assay can be used to e.g., determine TNF-alpha activity with actinomycin D-treated ME180 cells and L929 cells can be used in an assay described by Ostrove and Gifford (Proc. Soc . Ex . Biol. Med. 160, 354-358 (1979)), Aggarwal and Essalu (J. Biol. Chem. 262, 10000-10007 (1987)) and Levesque et al. (J. Immun. Meth. 178, 71-76 (1995)). L929 cells (CCLI: American Type Culture Collection) are maintained in McCoy's 5A medium containing 10% fetal bovine serum. Confluent cultures were treated briefly with 0.25% trypsin in physiological solution and resuspended in a fresh medium. The trypsinized cells per well are plated in 96-Well plates (Corning) and incubated for 24 hours at 37°C. Then actinomycin D was added to a final concentration of 0.25 ug/ml . Samples containing the compounds to be tested are added to the wells and incubation is continued overnight at the same temperature. After microscopic evaluation, the medium is decanted, and the wells are rinsed with PBS. The wells are then filled with a crystal violet dye solution. The dye is extracted and the absorbance of the dye retained in viable cells is determined at 570 nm.
Dosage levels of between about 0.01 and about 100 mg/kg body weight per day, preferably between about 0.5 and about 75 mg/kg body weight per day and most preferably between about 1 and about 50 mg/kg body weight per day of the active ingredient compound are useful in a monotherapy.
Typically, the pharmaceutical compositions of this invention will be administered from about 1 to 5 times per day or alternatively, as a continuous infusion. Such administration can be used as a chronic or acute therapy. The amount of active ingredient that may be combined with the carrier materials to produce a single dosage form will vary depending upon the host treated and the particular mode of administration. A typical preparation will contain from about 5% to about 95% active compound (w/w) . Preferably, such preparations contain from about 20% to about 80% active compound .
When the compositions of this invention comprise a combination of a compound of this invention and one or more additional therapeutic agents, both the compound and the additional agent should be present at dosage levels of between about 10% to about 80% of the dosage normally administered in a monotherapy regime. Upon improvement of a patient's condition, a maintenance dose of a compound, composition or combination of this invention may be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, may be reduced, as a function of the symptoms, to a level at which the improved condition is retained. When the symptoms have been alleviated to the desired level, treatment should cease. Patients may, however, require intermittent treatment on a long-term basis upon any recurrence or disease symptoms. It should also be understood that a specific dosage and treatment regimen for any particular patient will depend upon a variety of factors, including the activity of the specific compound employed, the age, body weight, general health, sex, diet, time of administration, rate of excretion, drug combination, and the judgment of the treating physician and the severity of the particular disease being treated. The amount of active ingredients will also depend upon the particular compound and other therapeutic agent, if present, in the composition.
Accordingly, a method for treating or preventing a disease of this invention in a subject comprises the step of administering to the subject any compound, pharmaceutical composition, or combination described herein.
In, a preferred embodiment, the invention provides a method of treating a mammal, having one of the aforementioned diseases, comprising the step of administering to said mammal a pharmaceutically acceptable composition described above. In this embodiment, if the patient is also administered another therapeutic agent, it may be delivered together with the compound of this invention in a single dosage form, or, as a separate dosage form. When administered as a separate dosage form, the other therapeutic agent may be administered prior to, at the same time as, or following administration of a pharmaceutically acceptable composition comprising a compound of this invention.
A kit according to this invention comprises a compound or pharmaceutical composition of this invention and a tool for measuring TNF-alpha levels or activity in vitro or in vivo . The kit can further comprise instructions for using the contents of the kit. A tool for measuring TNF-alpha levels or activity according to this invention refers to materials that can be used to measure the TNF gene product (i.e., RNA or protein) or activity. Such methods are described for example above. Thus, a tool according to this invention can include, e.g., an anti-TNF antibody, a TNF-alpha DNA probe or a genetically engineered cell line responsive to TNF-alpha levels described above. The methods for identifying a compound or composition that decreases TNF-alpha activity and/or levels according to this invention include methods for screening of a plurality of compounds or compositions for their ability to decrease TNF-alpha activity and/or levels. For example, high-throughput screening is a desired embodiment of this invention. According to one embodiment of this invention, high throughput screening can be achieved by having cells in culture in a plurality of wells in a microtiter plate, adding a different compound or composition to each well and comparing the TNF-alpha levels and/or activity in each cell culture to the TNF-alpha levels or activity present in a cell culture in a control well. Controls that are useful for the comparison step according to this invention include cells or subjects that have not been treated with a compound or composition and cells or subjects have been treated with a compound or composition that is known to have no effect on TNF- alpha levels or activity. According to one embodiment of this invention, the high throughput screening is automated so that the steps including the addition of the cells to the plate up to the data collection and analysis after addition of the compound or composition are done by machine . Instruments that are useful in the comparison step of this invention, e.g., instruments that can detect labeled objects (e.g., radiolabelled, fluorescent or colored objects) or objects that are themselves detectable, are commercially available and/or known in the art . Accordingly, compounds and compositions according to this invention that are useful for decreasing TNF-alpha levels and/or activity can be quickly and efficiently screened.
All applications, patents and references disclosed herein are incorporated by reference. In order that this invention be more fully understood, the following preparative and testing examples are set forth. These examples are for the purpose of illustration only and are not to be construed as limiting the scope of the invention in any way. Examples Example 1
[3S/R, (2S) ] -5-Fluoro-4-oxo-3-{ [1- (phenothiazine-10- carbonyl) piperidine-2 -carbonyl] amino} -pentanoic acid
Figure imgf000025_0001
Method A: (S) - (l-Phenothiazine-10-carbonyl) piperidine- 2 -carboxylic acid methyl ester
Figure imgf000025_0002
To a stirred solution of methyl pipecolate hydrochloride (lg, 5.57 mmol) in THF (10 ml) was added phenothiazine carbonyl chloride (1.457g, 5.57 mmol) followed by diisopropylethylamine (2.02 ml, 11.68 mmol) . The resulting solution was stirred for 16h before being partitioned between ethyl acetate and aq. sat. NH4C1. The organic layer was washed with brine, dried (MgS04) , filtered and evaporated. The residue was purified by flash chromatography (15% ethyl acetate in hexane) to afford the sub-title compound as a colorless oil which crystallized upon standing (1.823g, 89%) : 1H NMR (400MHz, CDCl3) δ 1.13-1.48 (3H, m) , 2.57-2.69 (2H, m) , 2.16 (1H, m) , 3.00 (1H, m) , 3.74 (4H, s+m) , 5.00 (1H, m) , 7.11 (2H, t) , 7.22-7.34 (4H, m) , 7.76 (2H, d) ;
13C NMR (100MHz, CDC13) δ 21.3 (CH2) , 24.8 (CH2) , 27.3 (CH2) , 44.9 (CH2) , 52.5 (CH3) , 55.9 (CH) , 122.8 (CH) , 125.5 (CH) , 127.8 (CH) , 128.0 (CH) , 129.2 (C) , 141.7 ( C) , 158 . 4 ( C ) , 172 . 2 ( C) .
Method B: (S) - (1-Phenothiazine-10-carbonyl) piperidine- 2 -carboxylic acid
Figure imgf000026_0001
To a stirred soltion of (S) - (1-phenothiazine-
10-carbonyl) piperidine-2 -carboxylic acid methyl ester (0.912g) in THF (15ml) and water (8ml) was added 2M NaOH (3.71 ml) and the reaction mixture was stirred for 16 hours. The reaction mixture was poured into sodium hydrogen carbonate solution (50 ml) and extracted with ethyl acetate (40 ml) . Aqueous phase was made acidic and extracted with ethyl acetate (2x75 ml) . Organic extracts were combined, dried (MgS04) and concentrated to give the sub-title compound as a white solid (0.709g, 81%): XH NMR (400MHz, CDCl3) δ 0.99-1.72 (5H, m) , 2.23 (IH, m) , 2.97 (IH, m) , 3.58 (IH, m) , 4.93 (IH, m) , 7.16 (2H, t) , 7.28 (2H, t) , 7.37 (2H, d) , 7.78 (2H, d) ; 13C NMR (100MHz, CDCl3) δ 21.0 (CH2) , 24.2 (CH2) , 26.7 (CH2) , 45.7 (CH2) , 56.0 (CH) , 123.8 (CH) , 126.0 (CH) , 127.9 (CH) , 128.1 (CH) , 130.3 (C) , 141.2 (C) , 160.1 (C) , 175.9 (C) .
Method C: [3S/R, 4S/R (2S) ] -5-Fluoro-4-hydroxy-3- { [1- (phenothiazine- 10 -carbonyl ) piperidine-2 - carbonyl] amino} -pentanoic acid tert-butyl ester
Figure imgf000027_0001
A stirred mixture of (S) - (l-phenothiazine-10- carbonyl) piperidine-2-carboxylic acid (233mg, 0.658mmol), 3-amino-5-fluoro-4-hydroxy-pentanoic acid tert-butyl ester (150mg, .724mmol), HOBt (98mg,
0.724mmol), DMAP (88mg, 0.724mmol) and anhydrous THF (10ml) was cooled to 0°C before EDC (139mg, 0.724mmol) was added. The mixture was allowed to warm to room temperature during 16h then concentrated under reduced pressure. The residue was purified by flash chromatography (50% ethyl acetate in hexane) to afford the sub-title compound as a pale pink foam (294mg, 82%): 1H NMR (400MHz, CDC13) δ 1.96 (IH, m) , 1.18-1.60 (13H, m) , 2.10-2.25 (IH, m) , 2.48-2.70 (2H, m) , 2.78- 2.94 (IH, m) , 3.51-4.72 (7H, m) , 7.03-7.36 (7H, m) ,
7.71-7.76 (2H, m) ; 19F (376MHz, CDCl3) δ -228.9 (t) , - 229.3 (t) , -230.1 (t) , -230.2 (t) .
Method D: [3S/R, (2S) ] -5-Fluoro-4-oxo-3- { [1- (phenothiazine-10 -carbonyl) piperidine-2- carbonyl] amino} -pentanoic acid tert-butyl ester
Figure imgf000027_0002
A stirred solution of [3S/R, 4S/R (2S) ] -5- Fluoro-4-hydroxy-3- { [1- (phenothiazine-10- carbonyl) piperidine-2-carbonyl] amino} -pentanoic acid tert-butyl ester (294mg, 0.541mmol) in anhydrous DCM (lOmL) was treated with 1 , 1, 1-triacetoxy-l, 1-dihydro- l,2-benziodoxol-3 (IH) -one (344mg, 0.812mmol) at 0°C. The resulting mixture was allowed to warm to room temperature over 2h, diluted with ethyl acetate, then poured into a 1:1 mixture of saturated aqueous sodium hydrogen carbonate and saturated aqueous sodium thiosulphate . The organic layer was removed and the aqueous layer was re-extracted with ethyl acetate. The combined organic extracts were dried (Na2S04) and concentrated. The residue was purified by flash chromatography (30% ethyl acetate in hexane) to afford the sub-title compound as a pale pink foam (220mg, 75%): 1H NMR (400MHz, CDCl3) δ 0.84-0.96 (IH, ) , 1.20- 1.40 (10H, m+2s) , 1.51-1.56 (3H, m) , 2.20-2.27 (IH, m) , 2.70-2.98 (3H, m) , 3.49-3.63 (IH, m) , 4.74-5.24 (4H, m) , 7.14-7.18 (2H, m) , 7.28-7.38 (4H, m) , 7.48-7.79 (3H, m) ; 13C (100MHz, CDCl3) δ 20.8/21.0 (CH2) , 23.7/23.9 (CH2) , 25.8/25.9 (CH2) , 28.2/28.3 (CH3) , 36.8/36.9 (CH2) , 46.0/46.1 (CH2) , 52.9 (CH) , 56.8 (CH) , 82.6 (C) ,
84.4/84.5 (2d, J 184.0/183.3 , CH2F) , 123.7/123.8 (CH) , 126.1 (CH) , 128.0/128.1 (CH) , 128.2/128.3 (CH) , 130.4/130.5 (C) , 141.4 (C) , 160.0 (C) , 170.0 (C) , 171.7 (C) , 202.9 (C) ; 19F (376MHz, CDCl3) δ-231.9 (t) , -232.2 (t) .
Method E: [3S/R, (2S) ] -5-Fluoro-4-oxo-3 - { [1- (phenothiazine-10-carbonyl) piperidine-2- carbonyl] amino} -pentanoic acid
Figure imgf000028_0001
Trifluoroacetic acid (5mL) was added to a stirred ice cold solution of [3S/R, (2S) ] -5-fluoro-4- oxo-3-{ [1- (phenothiazine-10-carbonyl) piperidine-2- carbonyl] amino} -pentanoic acid tert-butyl ester (130mg, 0.24mmol) in anhydrous DCM (5mL) . The mixture was stirred at 0°C for 0.5h then at room temperature for 0.5h. The mixture was concentrated under reduced pressure and then the residue was dissolved in dry DCM. This process was repeated several times in order to remove excess trifluoroacetic acid. The gum was lyophilized twice from HPLC grade water to afford the title compound as a white powder (77mg, 66%) -. IR
(solid) 1670, 1716, 1782 cm"1; ^Η NMR (400MHz, d6-DMSO) δ 0.96-0.99 (IH, m) , 1.23-1.26 (2H, m) , 1.42-1.44 (IH, m) , 1.60 (IH, m) , 1.91-1.98 (IH, m) , 2.51-2.89 (2H, m) , 3.11-3.22 (IH, m) , 3.57-3.60 (IH, m) , 4.30-4.72 and 5.05-5.29 (4H, 2m), 7.11-7.17 (2H, m) , 7.24-7.30 (2H, m) , 7.34-7.38 (2H, m) , 7.57-7.63 (2H, m) , 8.07-8.61 (IH, m) ; 13C NMR (100MHz, DMSO) δ (DMSO+TFA) 18.8/18.9 (CH2) , 22.2/22.3 (CH2) , 25.8/26.1 (CH2) , 31.5/33.2 (CH2) , 43^2 (CH2) , 50.6/51.1 (CH) , 54.4/54.5 (CH) , 82.8/82.9 (2d, J 178.6/178.1, CH2F) , 119.9/120.0 (CH) , 120.4/120.5 (CH) , 124.0/124.1 m(CH) , 125.9/126.0 (C) , 126.4/126.5 (CH) , 139.6/139.7 (C) , 156.0/156.4 (CO), 170.3 (CO), 170.7/170.8 (CO), 202.2/202.3 (2d, J 14.6/15.1, CO) . ; 19F (376MHz, DMSO) δ chemical shift (multiplicity, relative intensity) -226.7 (t, 3), -226.9 (t, 3), - 230.4 (t, 1), -231.2 (t, 1), -232.7 (t, 10), -233.0 (t, 10) . Example 2
[3S/R, (2S) ] -3-{ [1- (2-Chlorophenothiazine-10- carbonyl) iperidine-2 -carbonyl] amino} -4-f luoro-4-oxo- pentanoic acid
Figure imgf000030_0001
This was prepared from 2-chlorophenothiazine carbonyl chloride using procedures similar to those described above in Methods A-E (73mg, 69%) : IR (solid, cm"1) 1738, 1660, 1555, 1363, 1224 ; ^Η NMR (400MHz, d6- DMSO+TFA) δ 0.98-1.61 (4H, m) , 1.94-2.03 (IH, m) , 2.53- 2.89 (2H, m) , 3.12-3.24 (IH, m) , 3.51-3.61 (IH, m) , 4.31-4.73 and 5.10-5.24 (4H, 2m), 7.15-7.49 (6H, m) , 7.77-7.81 (IH, m) , 8.13-8.64 (IH, m) ; 13C NMR (100MHz, DMSO+TFA) δ 18.7/18.8 (CH2) , 22.3/22.6 (CH2) , 25.9/26.2 (CH2) , 31.5/33.2 (CH2) , 43.0/43.2 (CH2) , 50.6/51.1 (CH) , 54.4/54.5 (CH) , 82.8/82.9 (2d, J 178.7/178.3 , CH2F) , 119.3/119.8 (CH) , 120.2/120.3 (CH) , 123.6/123.7 (CH) , 124.4/124.5 (CH) , 124.6/124.8 (C) , 126.6 (CH) , 126.9 (CH) , 127.5 (CH) , 131.0 (C) , 139.2/139.2 (C) , 140.7/140.7 (C) , 155.5/155.9 (C) , 170.1/170.2 (C) ,
170.7/170.8 (C) , 201.2/201.3 (2d, J 14.3/13.9, CO); 19F NMR (376MHz, DMSO+TFA) δ -226.7 (t), -226.9 (t) , -230.3 (t) , -232.7 (t) , -233.0 (t) .
Example 3 [3S/R, (2S) ] -3-{ [1- (3 -Chlorophenothiazine-10- carbonyl) piperidine-2 -carbonyl] amino} -4-f luoro-4-oxo- pentanoic acid
Figure imgf000031_0001
This was prepared from 3-chlorophenothiazine carbonyl chloride using procedures similar to those described above in Methods A-E (108mg, 65%) : IR (solid, cm"1) 1737, 1655, 1455, 1373, 1224 ; XH NMR (400MHz, d6- DMSO+TFA) δ 0.99-1.61 (5H, m) , 1.91-2.04 (IH, m) , 2.54- 2.90 (2H, m) , 3.12-3.24 (IH, m) , 3.48-3.60 (IH, m) , 4.26-5.28 (4H, m) , 7.15-7.68 (7H, m) , 8.10-8.62 (IH, m) ; 13C NMR (100MHz, DMSO+TFA) δ 18.8 (CH2) , 22.2/22.3 (CH2) , 25.8 (CH2) , 33.1/33.2 (CH2) , 43.2 (CH2) , 50.6/51.0 (CH) , 54.3/54.4 (CH) , 82.7/82.8 (2d, CH2F) , 120.2/120.3 (CH) , 121.3/121.4 (CH) , 124.2/124.3 (CH) , 124.8/125.0 (C) , 125.7 (CH) , 126.3 (CH) , 126.6 (CH) , 126.8 (CH) , 127.7/127.9 (C) , 127.9/128.0 (C) , 138.5 (C) , 139.3 (C) , 156.0 (CO), 170.1 (CO), 170.6/170.7 (CO), 201.1/201.2 (2d, CO); 19F NMR(376MHz, DMSO+TFA) δ -226.6 (t) , -226.9 (t) , -232.6 (t) , -232.9 (t) .
Example 4
[3S/R, (2S) ] -3 - { [l - (3 , 4 -Dichlorophenothiazine- 10 - carbonyl ) piperidine-2 -carbonyl] amino} -4 -f luoro-4 -oxo- pentanoic acid
Figure imgf000031_0002
This was prepared from 3,4- dichlorophenothiazine carbonyl chloride using procedures similar to those described above in Methods A-E (91mg, 66%): IR (solid, cm"1) 1737, 1439, 1363, 1219; XH NMR (400MHz, ds-DMSO+TFA) δ 1.03-1.62 (5H, m) , 1.97-2.06 (IH, m) , 2.54-2.86 (2H, m) , 3.14-3.28 (IH, m) , 3.59-3.66 (IH, m) , 4.30-5.26 (4H, m) , 7.15-7.68 (6H, m) , 8.14-8.96 (IH, m) ; 13C NMR (100MHz, DMSO+TFA) δ 20.2 (CH2) , 23.8 (CH2) , 27.3 (CH2) , 34.6/34.7 (CH2) , 44.5 (CH2) , 52.1/52.5 (CH) , 55.7/55.9 (CH) , 84.2/84.3 (2d, CH2F) , 120.2/120.3 (CH) , 120.8/120.9 (CH) , 124.2/124.4 (C) , 125.9 (CH) , 127.7/127.8 (C) , 128.2 (CH) , 128.4/128.5 (C) , 128.8 (CH) , 128.9 (CH) , 140.0 (C) , 140.1 (C) , 140.6 (C) , 156.8/156.8 (CO), 171.5 (CO), 172.1/172.1 (CO), 202.6/202.7 (2d, CO); 19F NMR(376MHz, DMSO+TFA) δ -226.6 (t) , -226.8 (t) , -232.6 (t) , -232.9 (t) .
Example 5
[3S/R, (2S) ] -3- { [1- (2 , 6-Dichlorophenothiazine-10- carbonyl) piperidine-2 -carbonyl] amino} -4-fluoro-4-oxo- pentanoic acid
Figure imgf000032_0001
This was prepared from 2.7 g dichlorophenothiazine carbonyl chloride using procedures similar to those described above in Methods A-E (91mg, 70%) : IR (solid, cm"1) 1737, 1660, 1555, 1363, 1224; XH NMR (400MHz, d6-DMSO+TFA) δ 1.02-1.62
(5H, m) , 1.91-2.02 (IH, m) , 2.53-2.90 (2H, m) , 3.13- 3.25 (IH, m) , 3.51-3.62 (IH, m) , 4.31-5.29 (4H, m) , 7.22-7.75 (6H, m) , 8.18-8.65 (IH, m) ; 13C NMR (100MHz, DMSO+TFA) δ 20.2 (CH2) , 23.8 (CH2) , 27.3 (CH2) , 34.6
(CH2) , 44.7 (CH2) , 52.5 (CH) , 55.8 (CH) , 84.3 (d, J 178.2, CH2F) , 120.7/121.2 (CH) , 122.7/122.8 (CH) , 124.7/125.1 (C) , 125.3/125.4 (CH) , 127.4 (CH) , 128.1
(CH) , 128.7/128.9 (C) , 129.1 (CH) , 129.8 (C) , 132.7 (C) , 139.5/139.6 (C) , 141.8/141.9 (C) , 157.0 (CO) , 171.5 (CO) , 172.1 (CO) , 202.6 (d, J 14.3, CO) ; 19F NMR (376MHz, DMSO+TFA) δ -226.6 (t) , -226.9 (t) , -232.6
(t) , -232.9 (t) .
Example 6 [3S/R, (2S)]-3-{[l- (Carbazole-9-carbonyl) iperidine-2 - carbonyl] amino} -4-fluoro-4-oxo-pentanoic acid
Figure imgf000033_0001
This was prepared from 9-carbazole carbonyl chloride using procedures similar to those described above in Methods A-E (180mg, 75%) : IR (solid, cm"1) 1737, 1655, 1419, 1373, 1224 ; H NMR (400MHz, d6- DMSO+TFA) δ 1.36-1.65 (6H, m) , 1.94-1.99 (IH, m) , 2.12- 2.21 (IH, m) , 2.59-2.89 (2H, m) , 4.32-5.27 (4H, m) , 7.30-7.36 (2H, m) , 7.48-7.54 (2H, m) , 7.63-7.76 (2H, m) , 8.17-8.72 (3H, m) ; 13C NMR (100MHz, DMSO+TFA) δ 19.0 (CH2) , 23.7/23.8 (CH2) , 26.5/26.8 (CH2) , 33.3/33.5 (CH2) , 44.1 (br, CH2) , 50.9/51.4 (CH) , 54.5 (br, CH) , 82.9/83.1 (2d, J 178.7/178.7 , CH2F) , 111.0/111.1 (CH) , 111.9 (CH) , 119.5/119.7 (CH) , 120.6/120.7 (CH) , 122.5/122.7 (C) , 125.8/125.9 (CH) , 137.1/137.4 (C) , 153.2/153.3 (C) , 170.3/170.4 (C) , 170.8/170.9 (C) , 201.4/201.5 (2d, J 14.6/14.6, CO); 19F NMR (376MHz,
DMSO+TFA) δ d (J, %I) -226.6 (t, 3), -226.8 (t, 3), - 230.0 (t, 1), -232.7 (t, 10), -232.7 (t, 10).
Example 7
[3S/R, (2S) ] -5-Fluoro-4-oxo-3-{ [1- (6H-phenanthridine-5- carbonyl) -piperidine-2 -carbonyl] amino} -pentanoic acid
Figure imgf000034_0001
This was prepared from 9,10- dihydrophenanthrinine carbonyl chloride using procedures similar to those described above in Methods A-E (115mg, 61%) : IR (solid, cm"1) 1731, 1419, 1363,
1219; XH NMR (400MHz, d6-DMSO+TFA) δ 1.27-1.69 (5H, m) , 1.90-2.06 (IH, m) , 2.55-2.87 (2H, m) , 3.13-3.21 (2H, m) , 4.31-5.26 (6H, m) , 7.12-7.48 (6H, m) , 7.84-7.86 (2H, m) , 8.08-8.58 (IH, m) ; 13C NMR (100MHz, DMSO+TFA) δ 20.5 (CH2) , 24.2 (CH2) , 27.73 (CH2) , 34.6/34.8 (CH2) , 44.9 (CH2) , 48.5/48.7 (CH) , 52.1/52.5 (CH) , 55.4/55.7 (CH) , 84.2 (d, CH2F) , 120.2 (CH) , 123.3 (CH) , 123.6 (CH) , 124.7 (CH) , 126.1 (C),126.3 (CH) , 128.0 (CH) , 128.3 (CH) , 128.7 (CH) , 131.6 (C) , 134.6 (C) , 140.2 (C) , 172.1/172.2 (CO), 172.4/172.4 (CO), 203.0 (d, CO); 19F NMR(376MHz, DMSO+TFA) δ -226.8 (t) , -226.9 (t) , - 232.7 (t) , -232.9 (t) . Example 8
[3S/R, (2S) ] -5-Fluoro-3-{2- [ (lH-imidazole-2 -carbonyl) amino] -propionyla ino} -4-oxo-pentanoic acid, trifluoroacetate salt (Compound 1)
Figure imgf000035_0001
Method A:
(2S) -2- [ (Iff- Imidazole-2 -carbonyl) -amino] -propionic acid tert-butyl ester
To a solution of lH-imidazole-2 -carboxylic acid (0.17g) in N, N-dimethylformamide (DMF) (3mL) was added alanine tert-butyl ester hydrochloride (0.22g) , diisopropylethyl amine (0.27mL) and HOBT (0. 1g) before cooling to 0° C and the reaction mixture was then treated with EDC HCI (0.32g) . The cooling bath was removed and the reaction mixture was stirred at ambient temperature for 18 hrs before being diluted with ethyl acetate and washed with water and brine, dried (MgS0 ) and concentrated at reduced pressure. The residue was purified by silica gel chromatography (30% ethyl acetate in hexanes) to afford the sub-title compound as a colourless oil (0.263g, 73%): 1H NMR 400 MHz CDC13 1.50 (9H, s) , 1.51 (3H, d, J 7.2), 3.70 (IH, m) , 7.28 (2H, s) , 7.78 (IH, d, J 7.6) , 11.49 (IH, br s) .
Method B:
[3S/R, 4S/R, (2S) ] - 5-Fluoro-4 -hydroxy- 3 - {2 - [ (lff- imidazole-2 -carbonyl) -amino] -propionylamino } -pentanoic acid tert-butyl ester
Figure imgf000036_0001
A solution of (2S) -2- [ (Iff-imidazole-2-carbonyl) -amino] - propionic acid tert-butyl ester (0.257g) in dichloromethane (2ml) was cooled to 0° C before dropwise addition of trifluoroacetic acid and the reaction mixture was warmed to room temperature and stirred for 2hr before evaporation under reduced pressure. The residue was co-evaporated with dichloromethane (twice) and toluene (twice) to leave the required (2S) -2- [ (lH-imidazole-2-carbonyl) -amino] - propionic acid that was used without further purification (0.40g).
A solution of (2S) -2- [ (Iff-imidazole-2 - carbonyl) -amino] -propionic acid and 3~amino-5-fluoro-4- hydroxy-pentanoic acid tert-butyl ester (0.254g) in THF (7mL) was cooled to 0° C before addition of DMAP (0.151g), diisopropylethyl amine (0.56mL), HOBT (0.16g) and EDC HCI (0.23g). The reaction mixture was stirred at ambient temperature for 18 hrs before being concentrated at reduced pressure. The residue was purified by silica gel chromatography (5% methanol in dichloromethane) to afford the sub-title compound as a colourless solid (0.386g, 97%): U NMR 400MHz CDCI3/CD3OD 1.40 (12H, m) , 3.92 (IH, m) , 4.20-4.55 (4H, m) , 7.11 (2H, d, J 15); 19F NMR CDCl3 -229.74 (m) , - 229.84 (m) , -230.54 (m) , -230.87 (m) .
Method C:
[3 S/R, (2S) ] - 5 -Fluoro-3 - { 2 - [ ( lff-imidazole-2 -carbonyl) - amino] -propionylamino} -4 -oxo-pentanoic acid tert-butyl ester
Figure imgf000037_0001
A solution of [3S/R, (2S) ] -5-fluoro-4-hydroxy-3- {2- [ (lff- imidazole-2 -carbonyl) -amino] -propionylamino} -pentanoic acid tert-butyl ester (0.381g) in dichloromethane was cooled to 0°C before addition of 1,1,1 triacetoxy-1, 1- dihydro-1, 2-benziodoxol-3 (Iff) -one (0.476g). The mixture was stirred at room temperature for 2h before addition of an additional portion of 1,1,1 triacetoxy- 1, 1-dihydro-l, 2-benziodoxol-3 (Iff) -one (0.05g) and reaction mixture was then stirred for 90 min before being concentrated at reduced pressure. The residue was dissolved in ethyl acetate and washed with a 1:1 mixture of aqueous NaHS0 and aqueous Na2S03. The organic layer was collected, dried (MgS04) and concentrated. The residue was purified by flash chromatography (5% methanol in dichloromethane) to give the sub-title compound as a colourless foam (319mg, 84%): XH NMR 400MHz CDCl3 1.37+1.43 (9H, 2xs) , 1.54 (3H, m) , 2.85 (IH, m) , 3.03 (IH, m) , 4.85-5.30 (4H, m) , 7.18 (2H, d, J 16), 7.90 (IH, m) , 7.98 (IH, m) , 11.37+11.45 (IH, 2xs) ; 19F NMR 376 MHz CDCl3 -231.85 (t, J 48), - 232.12 (t, J 48) .
Method D : Compound 1
Figure imgf000037_0002
A solution of [3S/R, (2S) ] - 5 -Fluoro-3 - { 2 - [ (Iff-imidazole- 2 -carbonyl ) -amino] -propionylamino} -4 -oxo-pentanoic acid tert-butyl ester (0.31g) in dichloromethane (2ml) was cooled to 0° C before dropwise addition of trifluoroacetic acid and the reaction mixture was warmed to room temperature and stirred for 2hr before evaporation under reduced pressure. The residue was co-evaporated with dichloromethane (twice) and triturated under ether to give the title compound as a colourless solid (0.35g): IR 1785.7,1730.1,1653.7, 1538.1, 1458.2, 1384.2, 1268.7, 1188.4, 1150.9, 1053.3, 992.13, 931.8, 867.9, 847.0, 768.5 cm"1; 1H NMR 400MHz DMSO-d6 1.37 (3H, d) , 2.40-2.85 (2H,m, asp CH2) , 4.34- 4.75 (2.5H,m,2xCH+0.5CH2F) , 5.13-5.41 (1.5H,m, CH2F) , 7.50 (2H,s,iτnidazole CHs) , 8.58-8 , 79 (2H,m,NHs) , 13C NMR 100 MHz DMSO-dg 18.13, 18.85 (ala CH3) ; 33.13, 34.75 (asp CH2) , 48.68,52.41 (CHs) , 83.46, 85.21(CH2F), 123.67 (CH imidazole) , 139.57 , 158.86 , 172.35 (m) (C=0s) , 202.70(5 peaks ketone) ; 19F NMR 376 MHz DMSO-d6 decoupled 75.19 (3F,s,CF3COOH) , -(226.89, 226.96,230.80,231.59, 232.95, 233.06 (lF,6xs, COCH2F ring opened and ring closed) .
Example 9
[3S/R, (2S) ] -3- {2- [ (lff-Benzoimidazole-2-carbonyl) - amino] -propionylamino} -5-fluoro-4-oxo-pentanoic acid, trifluoroacetate salt (Compound 2)
Figure imgf000038_0001
This was prepared from lff-benzoimidazole-2 -carboxylic acid using procedures similar to those described in Methods A-D above (142 mg, 90% for final step) : (compound isolated as the TFA salt) off-white solid; IR (solid, cm"1) 3277.9, 1654.6, 1526.6, 1188.6, 1142.5, 1050.4, 927.5, 748.2, 712.4; XH NMR (DMSO-d6) 1.42 (3H, d) , 2.51-2.95 (2H, m) , 4.21-4.75 (2H, m) , 4.76-5.60 (3H, brm) , 7.41 (2H, m) , 7.65 (2H, m) , 8.21-9.05 (2H, m) ; 13C NMR (DMSO-dg) 18.0, 18.7, 18.8 (Ala CH3) , 37.2, 34.6,
34.7 (Asp CH2) , 47.6, 48.8, 48.85, 49.1 (Asp CH) , 52.0, 52.5 (Ala CH) , 83.5, 85.2, 85.3, 103.8, 106.0 (CH2F) ,
116.6, 123.9 (Aryl CH) , 145.3, 145.4, (Aryl C) , 158.4,
158.7, 158.8, 172.1, 172.2, 172.4, 172.5, 172.6, 172.7, 173.2 (NC=0) , 202.6, 202.7, 202.8, 202.9 (C=0) ; Found M+
364.1177. Cι6Hι7FN405 requires M+ 364.1183 (1.8 ppm) .
Example 10
[3S/R, (2S) ] -5-Fluoro-3-{2- [ (Iff-imidazole-2 -carbonyl) - amino] -butyrylamino} -4 -oxo-pentanoic acid, trifluoroacetate salt (Compound 3)
Figure imgf000039_0001
This was prepared from lff-benzoimidazole-2 -carboxylic acid using procedures similar to those described in Methods A-D above (147 mg, 64% for final step) : IR(cm"1) 3280.0, 1659.5, 157.9, 1192.5, 1141.6, 784.7, 721.1; XH NMR 400MHz DMS0-d6 0.95 (3H, m) , 1.78 (2H, m) , 2.58-2.98 (2H, m) , 4.30-4.78 (2.5H, m) , 5.10-5.42 (1.5H, m) , 7.41 (2H, s) , 8.44+8.75 (2H, 2xm) ; 13C NMR 100 MHz DMSO-d6 10.19, 10.29, 15.52 (CH3) , 25.42, 25.49, 26.03, 33.06, 33.13, 34.65, 34.80 (CH2) , 47.45, 47.53, 52.0, 53.96,
54.13 (CH) 65.27 (CH2) , 84.36 (d, J 177, CH2F) , 103.81, 104.00 (C) , 123.89 (CH) , 139.74 (C=0) , 156.90, 158.39, 158.74, 171.51, 171.80, 171.83, 172.02, 173.11 (C=0) , 202.51, 202.66, 202.76, 202.90 (CH2FC=0) ; 19F NMR 376 MHz DMSO-d6 -226.82 (t, J 45) , -226.84 (t, J 45) , - 230.67 (t, J 45) , -231.43 (t, J 45) , -232.79 (t, J 45) , -232.82 (t, J 45) .
Example 11
[3S/R, (2S) ] -5-Fluoro-3-{2- [ (lff-imidazole-2-carbonyl) - amino] -3 -methylbutyrylamino} -4 -oxo-pentanoic acid (Compound 4)
Figure imgf000040_0001
This was prepared from lff-benzoimidazole-2-carboxylic acid using procedures similar to those described in Methods A-D above (80g, 85% for final step) : white powder, IR (solid, cm"1) 1736, 1649, 1557, 1511, 1455, 1434, 1393; 1H NMR (DMSO+TFA) 0.92-0.95 (6H, m) , 2.06- 2.15 (IH, m) , 2.56-2.90 (2H, m) , 4.33-5.36 (4H, m) , 7.79 (2H, s) , 8.58-8.90 (2H, m) ; 19FNMR (DMSO+TFA) - 226.8 (t) , -230.6 (t) , -231.0 (t) , -232.5 (t) , -232.6
(t) ; 13C NMR (DMSO+TFA) 18.1/18.4 (CH3) , 19.2/19.3 (CH3) , 34.5/34.8 (CH2) , 51.9/52.2 (CH) , 58.5/58.8 (CH) ,
84.3/84.4 (2d, J 178.7/178.7, CH2F) , 122.0 (CH) , 137.5
(C) , 153.7 (C) , 170.6 (C) , 171.9/172.0 (C) , 202.5/202.8
(2d, J 14.6/14.6, CO) .
Example 12 [3S/R, (2S) ] -3- {2- [ (lff-Benzoimidazole-2-carbonyl) - amino] -3 -methylbutyrylamino} -5-fluoro- 4 -oxo-pentanoic acid (Compound 5)
Figure imgf000041_0001
This was prepared from lff-benzoimidazole-2 -carboxylic acid using procedures similar to those described in Methods A-D above (90mg, 87% for final step) : white powder, IR (solid, cm"1) 1737, 1665, 1527, 1373, 1194, 1137; IH NMR (DMSO-d6) 0.90-0.95 (6H, m) , 2.15-2.18 (IH, m) , 2.59-2.92 (2H, m) , 4.33-4.76 and 5.12-5.38 (4H, 2m), 7.31-7.35 (2H, m) , 7.66-7.68 (2H, m) , 8.36-8.82
(2H, m) ; 19FNMR (DMSO+TFA) -226.7 (t) , -226.9 (t) , - 232.4 (t) , -232.6 (t) ; 13C NMR (DMS0-d6) 18.3/18.4/18.5/18.7 (CH3) , 19.4/19.5 (CH3) , 31.0/31.1/31.6 (CH) , 34.7/34.8 (CH2), 51.8/52.1 (CH) , 57.9/58.3/58.6 (CH) , 84.3/84.4 (2d, J 178.7/178.7 , CH2F) , 124.0 (CH) , 145.2/145.2 (C) ,
158.4/158.5/158.7/158.8 (C) , 170.9/171.1/171.2 (C) , 172.0/172.0 (C) , 173.1 (C) , 173.9 (C) , 202.06/202.6
(2d, J 13.8, CO) .
Example 13
Figure imgf000041_0002
[3S/R(2S) ] -3- [2- (Carbazol-9-yl-2 -oxo-ethyl) - pentanoylamino] -5-fluoro-4-oxo-pentanoic acid Method A: (4S) -Benzyl-3-pentanoyl-oxazolidin-2-one
Figure imgf000042_0001
A solution of 4 (S) - (-) -benzyl -2 -oxazolidinone (lOg, 56.43mmol) in anhydrous THF (200ml) at -78°C was treated with a 2.5M solution of n-butyl lithium in hexanes (23.70ml, 59.26mmol) with stirring. The reaction mixture was allowed to stir at -78 °C for 30min before valeryl chloride (7.57ml, 62.10mmol) was added. The reaction mixture was then allowed to warm to ambient temperature over 15h after which it was diluted with NHC1 solution, diluted with ethyl acetate and washed with brine. The organic phase was dried (Na2S04) and concentrated to give a gum. This was purified by flash chromatography (10% EtOAc in 40/60 hexanes) to give the sub-title compound (14.61g, 99%) as a colourless oil: XH NMR (400MHz, CDC13) δ 0.94-1.20 (3H, m) , 1.35-1.50 (2H, m) , 1.62-1.80 (2H, m) , 2.74-2.84 (IH, m) , 2.86-3.08 (2H, m) , 3.27-3.39 (IH, m) , 4.11- 4.26 (2H, m) , 4.62-4.76 (IH, m) , 7.18-7.40 (5H, m) .
Method B: [4S (3R) ] -3- (4-Benzyl-2-oxo-oxazolidine-3- carbonyl) -hexanoic acid tert-butyl ester
Figure imgf000042_0002
A solution of (4S) -benzyl-3 -pentanoyl- oxazolidin-2-one (14.20g, 54.34mmol) in THF (100ml) at -78 °C was treated over lOmin with a IM solution of sodium bis (trimethylsilyl) amide in THF (59.80ml, 59.77mmol) l with stirring. The reaction mixture was allowed to stir at -78°C for 30min before tert-butyl bromoacetate (10.43ml, 70.64mmol) was added. The reaction mixture was then allowed to stir for a further 3.5h at -78 °C after which it was diluted with NH4C1 solution, diluted with ethyl acetate and washed sequentially with NaHC03 solution and brine. The organic phase was dried (Na2S04) and concentrated to give a gum. On standing a white solid was formed and this was recrystallized from 40/60 DCM/hexanes to give the sub-title compound (14.62g, 72%) as a white solid: XH NMR (400MHz, CDCl3) δ 0.81-1.20 (3H, m) , 1.21-1.76 (13H, m) , 2.41-2.55 (IH, m) , 2.66-2.92 (2H, m) , 3.27- 3.40 (IH, m) , 4.05-4.26 (2H, m) , 4.61-4.72 (IH, m) , 7.12-7.40 (5H, m) .
Method C: (2R) -2-Propyl-succinic acid 1-benzyl ester 4- tert-butyl ester
Figure imgf000043_0001
A solution of benzyl alcohol (4.62ml, 44.64mmol) in THF (80ml) at -20°C was treated with a 2.5M solution of n-butyl lithium in hexanes (13.36ml, 33.48mmol) with stirring. The reaction mixture was allowed to warm to -5°C over 40min before a solution of [4S (3R) ] -3- (4-benzyl-2-oxo-oxazolidine-3-carbonyl) - hexanoic acid tert-butyl ester (8.38g, 22.32mmol) in THF (20ml) was added. The reaction mixture was warmed to ambient temperature over 15h after which it was diluted with NH4C1 solution and ethyl acetate and washed with brine. The organic phase was dried (Na2S04) and concentrated to give a gum. This was purified by flash chromatography (11% EtOAc in 40/60 hexanes) to give the sub-title compound (4.56g, 67%) as a colourless oil: ^Η NMR (400MHz, CDC13) δ 0.83-1.00 (3H, m) , 1.21-1.71 (13H, m) , 2.34-2.45 (IH, m) , 2.75-2.95 (IH, m) , 5.09-5.25 (2H, m) , 7.30-7.43 (5H, m) .
Method D: (2R) -2 -Propyl -succinic acid 1-benzyl ester
Figure imgf000044_0001
A stirred solution of (2R) -2-propyl-succinic acid 1-benzyl ester 4- tert-butyl ester (4.56g,
14.88mmol) in anhydrous DCM (20ml) at 0°C was treated with a solution of trifluoroacetic acid (10ml) in anhydrous DCM (10ml) . The reaction mixture was allowed to warm to ambient temperature over 3h before being concentrated under reduced pressure. The residue was dissolved in dry DCM, before concentrating again. This process was repeated several times in order to remove excess trifluoroacetic acid to leave the sub-title compound (3.70g, 99%) as a gum: 1H NMR (400MHz, CDC13) δ 0.82-0.99 (3H, m) , 1.21-1.76 (4H, m) , 2.45-2.60 (IH, m) , 2.76-3.00 (2H, m) , 5.10-5.21 (2H, m) , 7.28-7.43 (5H, m) , 7.83-8.18 (IH, m) .
Method E: (2R) -2- (2-carbazol-9-yl-2-oxo-ethyl) - pentanoic acid benzyl ester
Figure imgf000045_0001
A stirred solution of carbazole (2.49g, 14.88mmol) in anhydrous THF (30ml) at -78 °C was treated with a 1.0M solution of lithium bis (trimethylsilyl) amide in THF (14.88ml, 14.88mmol) . The reaction mixture was allowed to warm to ambient temperature over 2h before being re-cooled to -78°C.
A solution of (2R) -2-propyl-succinic acid 1- benzyl ester (3.70g, 14.78mmol) in anhydrous DCM (20ml) , stirring at 0°C, was treated with oxalyl chloride (1.43ml, 16.37mmol) and DMF (14 drops). The reaction mixture was stirred at 0°C for lh before being concentrated in vacuo . The residue was dissolved in anhydrous THF (10ml) and added to the lithium anion of carbazole previously prepared at -78 °C. The reaction mixture was warmed to ambient temperature over 4Oh after which it was diluted with NH4C1 solution, and ethyl acetate and washed sequentially with 2N HCI, NaHC03 solution and brine. The organic phase was dried (Na2S04) and concentrated to give a gum which was purified by flash chromatography (10% EtOAc in 40/60 hexanes) to give the sub-title compound (4.50g, 76%) as a semi-solid/oil which also contained carbazole: 1H NMR (400MHz, CDCl3) δ 0.82-1.05 (3H, m) , 1.11-1.99 (4H, m) , 3.18-3.38 (2H, m) , 3.56-3.71 (IH, m) , 5.10-5.30 (2H, m) , 7.11-7.60 (9H, m) , 7.92-8.29 (4H, m) .
Method F: (2R) -2- (2-carbazol-9-yl-2-oxo-ethyl) - pentanoic acid
Figure imgf000046_0001
A stirred solution of (2R) -2- (2-carbazol-9- yl-2-oxo-ethyl) -pentanoic acid benzyl ester (4.50g, 11.26mmol) in EtOAc (60ml) was treated with 10% Pd on carbon (~400mg) and the reaction mixture was then placed under an atmosphere of hydrogen. After lh further 10% Pd on carbon (~300mg) was added and the reaction mixture was placed under hydrogen, with stirring, for a further 3h after which the reaction mixture was filtered through a celite pad and concentrated to give the sub-title compound (2.94g, 84%) as a white solid which also contained carbazole: """H NMR (400MHz, CDC13) δ 0.92-1.04 (3H, m) , 1.32-2.00 (4H, m) , 3.19-3.34 (2H, m) , 3.58-3.70 (IH, m) , 7.30-7.53 (4H, m) , 8.00-8.30 (4H, m) .
Method G: [3S/R, 4S/R, (2R) ] -3- [2- (2-carbazol-9-yl-2 - oxo-ethyl) -pentanoylamino] -5-fluoro-4-hydroxy-pentanoic acid tert-butyl ester
Figure imgf000046_0002
A stirred mixture of (2R) -2- (2-carbazol-9- yl-2 -oxo-ethyl) -pentanoic acid (2.94g, 9.50mmol), 3- amino-5-fluoro-4-hydroxy-pentanoic acid tert-butyl ester (2.07g, 9.99mmol), HOBT (1.41g, 10.43mmol), DMAP (1.34g, 10.97mmol) and anhydrous THF (40ml) was cooled to 0°C before EDC (2.00g, 10.43mmol) was added. The mixture was allowed to warm to room temperature during 16h then concentrated under reduced. pressure . The residue purified by flash chromatography (33% EtOAc in 40/60 hexanes) to give the sub-title compound (2.51g, 53%) as a foam: XH NMR (400MHz, CDC13) δ 0.90-1.03 (3H, m) , 1.20-1.90 (13H, m) , 2.50-3.00 (3H, m) , 3.12-3.26 (IH, m) , 3.59-3.80 (2H, m) , 4.00-4.68 (3H, m) , 6.53- 6.89 (IH, m) , 7.30-7.52 (4H, m) , 7.95-8.05 (2H, m) , 8.15-8.26 (2H, m) ; 19F NMR (376 MHz, CDCl3) -229.10, - 229.34, -230.95, -231.09.
Method H: [3S/R, (2R) ] -3- [2- (2-carbazol-9-yl-2-oxo- ethyl) -pentanoylamino] -5-fluoro-4 -oxo-pentanoic acid tert-butyl ester
Figure imgf000047_0001
A stirred solution of [3S/R, 4S/R, (2R)]-3- [2- (2-carbazol-9-yl-2-oxo-ethyl) -pentanoylamino] -5- fluoro-4-hydroxy-pentanoic acid tert-butyl ester (2.51g, 5.03mmol) in anhydrous DCM (60ml) was treated with 1, 1, 1 -triacetoxy-1, 1-dihydro-l, 2-benziodoxol- 3 (IH) -one (2.35g, 5.53mmol) at 0°C . The resulting mixture was kept at 0°C for 3h, diluted with DCM, and then washed sequentially with saturated aqueous sodium thiosulphate, NaHC03 solution and brine. The organics were dried (Na2S0 ) and concentrated. The residue was purified by flash chromatography (25% ethyl acetate in 40/60 hexanes) to afford the sub-title compound as an off white solid (1.437g, 57%) : IR (solid, cm"1) 1722, 1689, 1636, 1531, 1441, 1365, 1279, 1155; XH NMR (400MHz, CDC13) δ 0.85-1.50 (3H, m) , 1.35-1.54 (HH, m) , 1.55-1.69 (IH, m) , 1.78-1.95 (IH, m) , 2.67-3.28 (4H, m) , 3.60-3.79 (IH, m) , 4.80-5.59 (3H, m) , 6.89-7.04 (IH, m) , 7.33-7.54 (4H, m) , 7.98-8.04 (2H, m) , 8.15-
8.28 (2H, m) ; 13C (100MHz, CDC13) δ 14.12, 14.40, 14.47, 14.60, 20.78, 20.84, 21.47, 28.32, 28.42, 28.48, 29.77, 33.63, 34.58, 34.91, 40.05, 43.05, 43.26, 43.29, 52.60, 53.00, 53.64, 66.90, 66.99, 82.62, 82.69, 85.53, 116.88, 116.94, 120.28, 120.31, 124.27, 127.76, 127.86, 128.69, 128.77, 128.99, 138.80, 171.21, 171.29, 172.21, 172.25, 175.53, 176.03, 203.04, 203.20, 203.30, 203.46; 19F (376MHz, CDC13) δ -232.12 , -233.24.
Method I: [3S/R, (2R) ] -3- [2- (2-Carbazol-9-yl-2-oxo- ethyl) -pentanoylamino] -5-fluoro- -oxo-pentanoic acid
Figure imgf000048_0001
A solution of [3S/R, (2R) ] -3- [2- (2-carbazol- 9-yl-2-oxo-ethyl) -pentanoylamino] -5-fluoro-4-oxo- pentanoic acid tert-butyl ester (1.43g, 2.88mmol) in anhydrous DCM (20ml) was treated with a solution of TFA (10ml) in anhydrous DCM (10ml) with stirring. The mixture was stirred at 0°C for 2h then at room temperature for 2h. The mixture was concentrated under reduced pressure and then the residue was dissolved in dry DCM. This process was repeated several times in order to remove excess trifluoroacetic acid. The off- white solid was recrystallized from Et20 / 40/60 hexanes to give the title compound as a white powder (71mg) : IR (solid, cm"1) 1746, 1689, 1641, 1541, 1436, 1374, 1284, 1207, 1160cm-l; XH NMR (400MHz, d6-DMSO) δ 0.80-1.00
(3H, m) , 1.20-1.76 (4H, m) , 2.30-2.90 (2H, m) , 2.95- 3.24 (IH, m) , 3.26-3.59 (2H, m) , 4.25-4.79 (1.5H, m) , 5.02-5.43 (1.5H, m) , 7.36-7.58 (4H, m) , 8.10-8.30 (4H, m) , 8.54-8.91 (IH, m) ; 13C NMR (100MHz, DMSO) δ 14.31, 20.03, 20.13, 21.92, 22.51, 34.36, 34.77, 41.20, 41.62, 44.06, 51.77, 52.84, 83.45, 85.22, 116.70, 120.54, 123.91, 124.01, 127.85, 126.01, 138.20, 172.15, 172.36, 172.96, 173.00, 175.32, 175.48, 202.60, 203.10; 19F
(376MHz, DMSO) δ -226.68, -226.73, -231.21, -232.95, - 233.38, -233.52.
Example 14
Figure imgf000049_0001
[3S/R(2S) ] -3- [2- (2-Carbazol-9-yl-2 -oxo-ethyl) -3 -methylbutyrylamino] -5-fluoro- -oxo-pentanoic acid
This was prepared using procedures similar to those described in Methods A- I. The product was isolated as a white powder (71% for final step) : IR (solid, cm"1) 1739, 1682, 1646, 1545, 1447, 1381, 1290, 1209, 1170 cm"1; E NMR (400MHz, DMSO + TFA) δ 0.79-1.08 (6H, m) , 1.89-2.15 (IH, m) , 2.31-3.60 (5H, m) , 4.21- 4.78 (1.25H, m) , 4.98-5.45 (1.75H, m) , 7.38-7.60 (4H, m) , 8.14-8.35 (4H, m) , 8.56-8.90 (IH, m) ; 13C NMR (100MHz, DMSO) δ 20.46, 20.84, 21.04, 21.21, 30.77,
30.85, 33.37, 34.83, 35.24, 38.16, 38.89, 47.67, 48.23, 52.19, 53.43, 83.96, 84.01, 85.72, 85.77, 117.16, 121.02, 124.43, 126.42, 126.52, 128.42, 138.75, 172.64, 172.90, 173.85, 173.90, 174.74, 174.93, 175.16, 202.91, 203.04, 203.51, 203.65; 19F (376MHz, DMSO) δ -226.63, - 226.68, -231.24, -233.16, -233.38, -233.55.
Biological Method
Example 15 : Inhibition of TNF release from whole blood
Human blood was freshly drawn from healthy donors and collected in vacutainers . Blood was diluted 1 : 2 in PBS (tissue culture, pyrogen free) in a sterile bottle and inverted to mix well. Aliquots of 0.5 ml of blood mixture were dispensed into cluster tubes in 96 well format.
Dilutions of the test compounds were prepared in RPMI by taking lOOmM DMSO stocks of the compounds and diluting 1:10 in RPMI medium in eppendorfs, to give a lOmM stock. 1:5 serial dilutions were prepared from the stock solutions.
LPS was kept at a frozen stock (-20 degrees C) at lmg/ml in PBS and then diluted to 1:10 with RPMI medium and finally diluted in the medium again 1:350. 50μl of each test compound (first concentration was lOOuM) were added to the blood samples and then stimulated with 10 μl LPS (final concentration in the well is 5ng/ml) . The. contents were gently mixed using an 8 well multi-channel pipette and incubated at 37°C overnight. At the end of the incubation time, contents were gently mixed, then spun down at 1000 x g for 5 mins at 20°C. The serum supernatants were transferred to a fresh plate without disturbing the RBCs and diluted 1:2 with the diluent RD6C. TNF-alpha levels of supernatants were assayed using the R+D systems ELISA kit, using R+D systems protocol. Samples were read at 450nm. The compounds shown in Table 1 were tested in the above assay. In this TNF-alpha assay, category "A" indicates an IC50 value of < 500 nM. Category "B" indicates an IC50 value of 500-1000 nM. Category "C" indicates an IC50 value of 1001-2000 nM. category "D" indicates an IC50 value of > 2000 nM. See Table 1.
Table 1. TNF-alpha IC50 Data of Exemplary Compounds
Figure imgf000051_0001
Figure imgf000052_0001
Figure imgf000053_0001
Figure imgf000054_0001
Figure imgf000055_0001
Figure imgf000056_0001
While we have described a number of embodiments of this invention, it is apparent that our basic examples may be altered to provide other embodiments, which utilize the compounds and methods of this invention. Therefore, it will be appreciated that the scope of this invention is to be defined by the appended claims rather than by the specific embodiments, which have been represented by way of example .

Claims

CLAIMS We claim:
1. A method for identifying a compound that decreases TNF-alpha levels in a cell culture comprising the steps of administering a compound according to any one of FIGs . 1-20 or a pharmaceutical composition comprising the compound to the cell culture and comparing the amount of TNF-alpha present in the cell culture to the amount of TNF-alpha present in a cell culture that has not been treated with the compound.
2. The method according to claim 1, wherein the compound has an IC50 of 6 μM or less.
3. A method for identifying a compound that decreases TNF-alpha activity in a cell culture comprising the steps of administering a compound according to any one of FIGs. 1-20 or a pharmaceutical composition comprising the compound to the cell culture and comparing the activity of TNF-alpha present in the cell culture to the activity of TNF-alpha present in a cell culture that has not been treated with the compound .
4. The method according to claim 3, wherein the compound has an IC50 of 6 μM or less.
5. A method for identifying a compound for decreasing TNF-alpha levels in a subject comprising administering a compound according to any one of FIGs. 1-20 or a pharmaceutical composition comprising the compound and comparing the TNF-alpha levels present in the subject before and after treatment with the compound .
6. A method for identifying a compound for decreasing TNF-alpha activity in a subject comprising administering a compound according to any one of FIGs. 1-20 or a pharmaceutical composition comprising the compound and comparing the TNF-alpha activity present in the subject before and after treatment with the compound .
7. A kit comprising a caspase inhibitor and a tool for measuring TNF-alpha levels or activity.
8. A kit of claim 7 wherein said caspase inhibitor is a compound according to any one of FIGs. 1-20.
9. A method for inhibiting TNF-alpha activity or decreasing TNF-alpha activity in a subject in need thereof comprising administering a compound according to any one of FIGs. 1-20 or a pharmaceutical composition comprising said compound to said subject.
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