WO2003080857A2 - Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis - Google Patents
Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis Download PDFInfo
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- WO2003080857A2 WO2003080857A2 PCT/US2003/008890 US0308890W WO03080857A2 WO 2003080857 A2 WO2003080857 A2 WO 2003080857A2 US 0308890 W US0308890 W US 0308890W WO 03080857 A2 WO03080857 A2 WO 03080857A2
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- oligomer
- pna
- seq
- pna oligomer
- oligomers
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
Definitions
- Bacillus anthracis or the rRNA or rDNA associated therewith. It is to be understood that the foregoing characteristics are not mutually exclusive.
- Bacillus anthracis or the rRNA or rDNA associated therewith, in the sample.
- Blocking probes may also be used as a means to improve discrimination beyond the limits possible by mere optimization of stringency factors. Suitable hybridization conditions will thus comprise conditions under which the desired degree of discrimination is achieved such that an assay generates an accurate (within the tolerance desired for the assay) and reproducible result.
- Figure 1A and Figure IB are color images of B. anthracis (1A) and B. pseudomycoides (IB) analyzed by fluorescence in situ hybridization using Bant23S02/Flu. Organisms of B. anthracis are identified by the fluorescein-labeled PNA probe and thus appear bright green fluorescent in Figure 1A, whereas organisms of B. pseudomycoides in Figure IB appear faint red.
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2003578581A JP2005520554A (en) | 2002-03-21 | 2003-03-21 | PNA oligomers, oligomer sets, methods and kits for the detection of Bacillus Anthracis |
EP03723808A EP1487858A4 (en) | 2002-03-21 | 2003-03-21 | Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis |
AU2003230717A AU2003230717A1 (en) | 2002-03-21 | 2003-03-21 | Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis |
CA002479594A CA2479594A1 (en) | 2002-03-21 | 2003-03-21 | Pna oligomers, oligomer sets, methods and kits pertaining to the detection of bacillus anthracis |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US36642402P | 2002-03-21 | 2002-03-21 | |
US60/366,424 | 2002-03-21 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003080857A2 true WO2003080857A2 (en) | 2003-10-02 |
WO2003080857A3 WO2003080857A3 (en) | 2004-04-08 |
Family
ID=28454797
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2003/008890 WO2003080857A2 (en) | 2002-03-21 | 2003-03-21 | Pna oligomers, oligomers sets, methods and kits pertaining to the detection of bacillus anthracis |
Country Status (6)
Country | Link |
---|---|
US (1) | US8017758B2 (en) |
EP (1) | EP1487858A4 (en) |
JP (1) | JP2005520554A (en) |
AU (1) | AU2003230717A1 (en) |
CA (1) | CA2479594A1 (en) |
WO (1) | WO2003080857A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007045998A2 (en) * | 2005-07-01 | 2007-04-26 | Dako Denmark A/S | New nucleic acid base pairs |
CN102605084A (en) * | 2012-03-29 | 2012-07-25 | 昆明理工大学 | Fluorescence in situ hybridization (FISH) method for detecting bacillus cereus and bacillus subtitles |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2506151C (en) | 2002-11-15 | 2010-08-03 | Gen-Probe Incorporated | Assay and compositions for detection of bacillus anthracis nucleic acid |
JPWO2008018305A1 (en) * | 2006-08-08 | 2009-12-24 | アークレイ株式会社 | Mutation detection method and kit used therefor |
WO2008039367A1 (en) | 2006-09-22 | 2008-04-03 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Synthesis of trans-tert-butyl-2-aminocyclopentylcarbamate |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997012995A1 (en) * | 1995-10-06 | 1997-04-10 | Perseptive Biosystems, Inc. | Methods and kit for hybridization analysis using peptide nucleic acid probes |
US6448016B1 (en) * | 2001-06-12 | 2002-09-10 | The United States Of America As Represented By The Secretary Of The Army | Nucleotide sequences for detection of Bacillus anthracis |
US6569630B1 (en) * | 1999-07-02 | 2003-05-27 | Conceptual Mindworks, Inc. | Methods and compositions for aptamers against anthrax |
Family Cites Families (26)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6228982B1 (en) * | 1992-05-22 | 2001-05-08 | Benget Norden | Double-stranded peptide nucleic acids |
DK51092D0 (en) * | 1991-05-24 | 1992-04-15 | Ole Buchardt | OLIGONUCLEOTIDE ANALOGUE DESCRIBED BY PEN, MONOMERIC SYNTHONES AND PROCEDURES FOR PREPARING THEREOF, AND APPLICATIONS THEREOF |
US5719262A (en) * | 1993-11-22 | 1998-02-17 | Buchardt, Deceased; Ole | Peptide nucleic acids having amino acid side chains |
US5641625A (en) * | 1992-05-22 | 1997-06-24 | Isis Pharmaceuticals, Inc. | Cleaving double-stranded DNA with peptide nucleic acids |
US5539082A (en) * | 1993-04-26 | 1996-07-23 | Nielsen; Peter E. | Peptide nucleic acids |
US5714331A (en) * | 1991-05-24 | 1998-02-03 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility |
US5766855A (en) * | 1991-05-24 | 1998-06-16 | Buchardt, Deceased; Ole | Peptide nucleic acids having enhanced binding affinity and sequence specificity |
GB9211979D0 (en) * | 1992-06-05 | 1992-07-15 | Buchard Ole | Uses of nucleic acid analogues |
US5527675A (en) * | 1993-08-20 | 1996-06-18 | Millipore Corporation | Method for degradation and sequencing of polymers which sequentially eliminate terminal residues |
DE4331012A1 (en) * | 1993-09-13 | 1995-03-16 | Bayer Ag | Nucleic acid-binding oligomers with N-branching for therapy and diagnostics |
GB2284209A (en) * | 1993-11-25 | 1995-05-31 | Ole Buchardt | Nucleic acid analogue-induced transcription of RNA from a double-stranded DNA template |
DK145493D0 (en) * | 1993-12-23 | 1993-12-23 | Dako As | ANTIBODY |
CA2214430A1 (en) * | 1995-03-04 | 1996-09-12 | Boehringer Mannheim Gmbh | Sequence-specific detection of nucleic acids |
US6475721B2 (en) * | 1995-03-04 | 2002-11-05 | Boston Probes, Inc. | Sequence specific detection of nucleic acids using a solid carrier bound with nucleic acid analog probes |
US6280964B1 (en) * | 1995-04-14 | 2001-08-28 | The Regents Of The University Of California | Binding sites for phosphotyrosine binding domains |
US6110676A (en) * | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
CA2218439A1 (en) * | 1996-12-21 | 1998-06-21 | Henrik Orum | Method of identifying a nucleic acid using triple helix formation of adjacently annealed probes |
US6107470A (en) * | 1997-05-29 | 2000-08-22 | Nielsen; Peter E. | Histidine-containing peptide nucleic acids |
US6485901B1 (en) * | 1997-10-27 | 2002-11-26 | Boston Probes, Inc. | Methods, kits and compositions pertaining to linear beacons |
WO1999022018A2 (en) * | 1997-10-27 | 1999-05-06 | Boston Probes, Inc. | Methods, kits and compositions pertaining to pna molecular beacons |
US6326479B1 (en) * | 1998-01-27 | 2001-12-04 | Boston Probes, Inc. | Synthetic polymers and methods, kits or compositions for modulating the solubility of same |
US6361942B1 (en) * | 1998-03-24 | 2002-03-26 | Boston Probes, Inc. | Method, kits and compositions pertaining to detection complexes |
US6287772B1 (en) * | 1998-04-29 | 2001-09-11 | Boston Probes, Inc. | Methods, kits and compositions for detecting and quantitating target sequences |
US6441152B1 (en) * | 1998-12-08 | 2002-08-27 | Boston Probes, Inc. | Methods, kits and compositions for the identification of nucleic acids electrostatically bound to matrices |
US5948680A (en) * | 1998-12-17 | 1999-09-07 | Isis Pharmaceuticals Inc. | Antisense inhibition of Elk-1 expression |
EP1356113B1 (en) * | 2000-12-15 | 2012-07-18 | Life Technologies Corporation | Methods for determining organisms |
-
2003
- 2003-03-21 EP EP03723808A patent/EP1487858A4/en not_active Withdrawn
- 2003-03-21 JP JP2003578581A patent/JP2005520554A/en not_active Withdrawn
- 2003-03-21 WO PCT/US2003/008890 patent/WO2003080857A2/en not_active Application Discontinuation
- 2003-03-21 US US10/393,855 patent/US8017758B2/en active Active
- 2003-03-21 AU AU2003230717A patent/AU2003230717A1/en not_active Abandoned
- 2003-03-21 CA CA002479594A patent/CA2479594A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997012995A1 (en) * | 1995-10-06 | 1997-04-10 | Perseptive Biosystems, Inc. | Methods and kit for hybridization analysis using peptide nucleic acid probes |
US6569630B1 (en) * | 1999-07-02 | 2003-05-27 | Conceptual Mindworks, Inc. | Methods and compositions for aptamers against anthrax |
US6448016B1 (en) * | 2001-06-12 | 2002-09-10 | The United States Of America As Represented By The Secretary Of The Army | Nucleotide sequences for detection of Bacillus anthracis |
Non-Patent Citations (1)
Title |
---|
See also references of EP1487858A2 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2007045998A2 (en) * | 2005-07-01 | 2007-04-26 | Dako Denmark A/S | New nucleic acid base pairs |
WO2007045998A3 (en) * | 2005-07-01 | 2008-04-10 | Dako Denmark As | New nucleic acid base pairs |
US8481697B2 (en) | 2005-07-01 | 2013-07-09 | Dako Denmark A/S | Nucleic acid base pairs |
CN102605084A (en) * | 2012-03-29 | 2012-07-25 | 昆明理工大学 | Fluorescence in situ hybridization (FISH) method for detecting bacillus cereus and bacillus subtitles |
Also Published As
Publication number | Publication date |
---|---|
JP2005520554A (en) | 2005-07-14 |
AU2003230717A1 (en) | 2003-10-08 |
CA2479594A1 (en) | 2003-10-02 |
WO2003080857A3 (en) | 2004-04-08 |
EP1487858A2 (en) | 2004-12-22 |
US8017758B2 (en) | 2011-09-13 |
US20030232402A1 (en) | 2003-12-18 |
EP1487858A4 (en) | 2006-01-04 |
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