WO2003076580A3 - High throughput mutagenesis screening method - Google Patents

High throughput mutagenesis screening method Download PDF

Info

Publication number
WO2003076580A3
WO2003076580A3 PCT/US2003/006908 US0306908W WO03076580A3 WO 2003076580 A3 WO2003076580 A3 WO 2003076580A3 US 0306908 W US0306908 W US 0306908W WO 03076580 A3 WO03076580 A3 WO 03076580A3
Authority
WO
WIPO (PCT)
Prior art keywords
mutagenesis methods
high throughput
screening method
variants
invention provides
Prior art date
Application number
PCT/US2003/006908
Other languages
French (fr)
Other versions
WO2003076580A2 (en
Inventor
Richard R Bott
James T Kellis Jr
Thomas B Morrison
Original Assignee
Richard R Bott
Genencor Int
James T Kellis Jr
Thomas B Morrison
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US60/362,372 priority Critical
Priority to US36237202P priority
Priority to US10/092,227 priority
Priority to US10/091,912 priority patent/US20030199068A1/en
Priority to US10/091,912 priority
Priority to US10/092,227 priority patent/US20030171543A1/en
Priority to US40392102P priority
Priority to US60/403,921 priority
Application filed by Richard R Bott, Genencor Int, James T Kellis Jr, Thomas B Morrison filed Critical Richard R Bott
Priority claimed from DK03744218T external-priority patent/DK1543117T3/en
Publication of WO2003076580A2 publication Critical patent/WO2003076580A2/en
Publication of WO2003076580A3 publication Critical patent/WO2003076580A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y301/00Hydrolases acting on ester bonds (3.1)
    • C12Y301/01Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01074Cutinase (3.1.1.74)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)

Abstract

The present invention provides mutagenesis methods for protein engineering. In particular, the present inventionprovides mutagenesis methods that include feedback adjustment from systematic result evaluation. More specifically, the invention provides site-saturation mutagenesis methods that screen for variants with one or more desirable protein properties and evaluates screening results to provide feedback for repeat screening and construction of new libraries. The invention further provides cutinase variants that provide improved stability and activity compared with the wild type cutinase.
PCT/US2003/006908 2002-03-05 2003-03-05 High throughput mutagenesis screening method WO2003076580A2 (en)

Priority Applications (8)

Application Number Priority Date Filing Date Title
US36237202P true 2002-03-05 2002-03-05
US10/092,227 2002-03-05
US10/091,912 US20030199068A1 (en) 2002-03-05 2002-03-05 High throughput mutagenesis screening method
US10/091,912 2002-03-05
US10/092,227 US20030171543A1 (en) 2002-03-05 2002-03-05 High throughput mutagenesis screening method
US60/362,372 2002-03-05
US40392102P true 2002-08-15 2002-08-15
US60/403,921 2002-08-15

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DK03744218T DK1543117T3 (en) 2002-03-05 2003-03-05 Mutagenesis screening procedure with high capacity
DE2003629504 DE60329504D1 (en) 2002-03-05 2003-03-05 Mutagenesis screening process with high throughput
AT03744218T AT444355T (en) 2002-03-05 2003-03-05 Mutagenesis screening process with high throughput
AU2003216540A AU2003216540A1 (en) 2002-03-05 2003-03-05 High throughput mutagenesis screening method
EP20030744218 EP1543117B1 (en) 2002-03-05 2003-03-05 High throughput mutagenesis screening method

Publications (2)

Publication Number Publication Date
WO2003076580A2 WO2003076580A2 (en) 2003-09-18
WO2003076580A3 true WO2003076580A3 (en) 2005-04-28

Family

ID=27808762

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2003/006908 WO2003076580A2 (en) 2002-03-05 2003-03-05 High throughput mutagenesis screening method

Country Status (7)

Country Link
EP (1) EP1543117B1 (en)
AT (1) AT444355T (en)
AU (1) AU2003216540A1 (en)
DE (1) DE60329504D1 (en)
ES (1) ES2333789T3 (en)
PT (1) PT1543117E (en)
WO (1) WO2003076580A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0709978A2 (en) 2006-04-14 2011-08-02 Genencor Int treatment in one stage of textile products
EP2171055B1 (en) 2007-06-06 2016-03-16 Danisco US Inc. Methods for improving multiple protein properties
RU2569106C2 (en) * 2007-06-06 2015-11-20 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Methods of improving protein efficiency
EP2537918A1 (en) 2011-06-20 2012-12-26 The Procter and Gamble Company Consumer products with lipase comprising coated particles
CN104204179A (en) 2011-06-20 2014-12-10 诺维信公司 Particulate composition
US9476072B2 (en) 2012-05-14 2016-10-25 Novozymes A/S Cutinase variants and polynucleotides encoding same
EP2767579B1 (en) 2013-02-19 2018-07-18 The Procter and Gamble Company Method of laundering a fabric
EP2767582A1 (en) 2013-02-19 2014-08-20 The Procter and Gamble Company Method of laundering a fabric
EP2767581A1 (en) 2013-02-19 2014-08-20 The Procter & Gamble Company Method of laundering a fabric
BR112016013511A2 (en) * 2013-12-11 2018-01-09 Novozymes As cutinase and polynucleotide variants that encode
EP2987848A1 (en) 2014-08-19 2016-02-24 The Procter & Gamble Company Method of laundering a fabric
EP2987849A1 (en) 2014-08-19 2016-02-24 The Procter and Gamble Company Method of Laundering a Fabric
BR112017027405A2 (en) 2015-06-26 2018-08-28 Unilever Nv detergent composition for washing clothes and domestic method of treating a fabric

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352594A (en) * 1984-05-29 1994-10-04 Genecor, Inc. Selection and method of making enzymes for perhydrolysis system and for altering substrate specificity, specific activity and catalytic efficiency

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU621813B2 (en) * 1986-11-19 1992-03-26 Genencor Inc. Novel hydrolase and method

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5352594A (en) * 1984-05-29 1994-10-04 Genecor, Inc. Selection and method of making enzymes for perhydrolysis system and for altering substrate specificity, specific activity and catalytic efficiency

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BOSTON M. ET AL.: "Structure and function of engineered Pseudomonas mendocina lipase", METHODS ENZYMOL., vol. 284, 1997, pages 298 - 317, XP008044067 *
See also references of EP1543117A4 *

Also Published As

Publication number Publication date
EP1543117A4 (en) 2007-06-20
WO2003076580A2 (en) 2003-09-18
EP1543117B1 (en) 2009-09-30
PT1543117E (en) 2010-01-07
ES2333789T3 (en) 2010-03-01
DE60329504D1 (en) 2009-11-12
AU2003216540A1 (en) 2003-09-22
EP1543117A2 (en) 2005-06-22
AT444355T (en) 2009-10-15
AU2003216540A8 (en) 2003-09-22

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