WO2003064691A3 - Methods and means for amplifying nucleic acid - Google Patents
Methods and means for amplifying nucleic acid Download PDFInfo
- Publication number
- WO2003064691A3 WO2003064691A3 PCT/IB2003/000843 IB0300843W WO03064691A3 WO 2003064691 A3 WO2003064691 A3 WO 2003064691A3 IB 0300843 W IB0300843 W IB 0300843W WO 03064691 A3 WO03064691 A3 WO 03064691A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- methods
- nucleic acid
- oligonucleotides
- pcr
- electrophoresis
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 5
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 abstract 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 3
- 238000003752 polymerase chain reaction Methods 0.000 abstract 3
- 238000001962 electrophoresis Methods 0.000 abstract 2
- 230000003321 amplification Effects 0.000 abstract 1
- 238000011835 investigation Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 108020004999 messenger RNA Proteins 0.000 abstract 1
- 238000007857 nested PCR Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2003206095A AU2003206095A1 (en) | 2002-01-29 | 2003-01-28 | Methods and means for amplifying nucleic acid |
JP2003564281A JP2005515792A (en) | 2002-01-29 | 2003-01-28 | Methods and means for manipulating nucleic acids |
EP03702979A EP1476569A2 (en) | 2002-01-29 | 2003-01-28 | Methods and means for manipulating nucleic acid |
CA002474864A CA2474864A1 (en) | 2002-01-29 | 2003-01-28 | Methods and means for amplifying nucleic acid |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US35221502P | 2002-01-29 | 2002-01-29 | |
US60/352,215 | 2002-01-29 |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003064691A2 WO2003064691A2 (en) | 2003-08-07 |
WO2003064691A3 true WO2003064691A3 (en) | 2003-11-27 |
Family
ID=27663061
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/IB2003/000843 WO2003064691A2 (en) | 2002-01-29 | 2003-01-28 | Methods and means for amplifying nucleic acid |
Country Status (6)
Country | Link |
---|---|
US (1) | US20030175908A1 (en) |
EP (1) | EP1476569A2 (en) |
JP (1) | JP2005515792A (en) |
AU (1) | AU2003206095A1 (en) |
CA (1) | CA2474864A1 (en) |
WO (1) | WO2003064691A2 (en) |
Families Citing this family (45)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20050196792A1 (en) * | 2004-02-13 | 2005-09-08 | Affymetrix, Inc. | Analysis of methylation status using nucleic acid arrays |
US7901882B2 (en) | 2006-03-31 | 2011-03-08 | Affymetrix, Inc. | Analysis of methylation using nucleic acid arrays |
WO2008123019A1 (en) * | 2007-03-05 | 2008-10-16 | Olympus Corporation | Method of detecting change in gene and detection device |
US9921154B2 (en) | 2011-03-18 | 2018-03-20 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
AU2012231098B2 (en) * | 2011-03-18 | 2016-09-29 | Bio-Rad Laboratories, Inc. | Multiplexed digital assays with combinatorial use of signals |
ES2904816T3 (en) * | 2012-02-27 | 2022-04-06 | Becton Dickinson Co | Molecular counting compositions |
US9970052B2 (en) | 2012-08-23 | 2018-05-15 | Bio-Rad Laboratories, Inc. | Digital assays with a generic reporter |
PL225633B1 (en) | 2012-11-22 | 2017-05-31 | Univ Jagielloński | Method for identification of RNA viruses and the application of the method |
KR102402446B1 (en) | 2013-08-28 | 2022-05-30 | 벡톤 디킨슨 앤드 컴퍼니 | Massively parallel single cell analysis |
US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
WO2016134078A1 (en) | 2015-02-19 | 2016-08-25 | Becton, Dickinson And Company | High-throughput single-cell analysis combining proteomic and genomic information |
US9727810B2 (en) | 2015-02-27 | 2017-08-08 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
EP3277843A2 (en) | 2015-03-30 | 2018-02-07 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
CN107580632B (en) | 2015-04-23 | 2021-12-28 | 贝克顿迪金森公司 | Methods and compositions for whole transcriptome amplification |
WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
EP3347465B1 (en) | 2015-09-11 | 2019-06-26 | Cellular Research, Inc. | Methods and compositions for nucleic acid library normalization |
WO2017192387A1 (en) | 2016-05-02 | 2017-11-09 | Cellular Research, Inc. | Accurate molecular barcoding |
US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
WO2017205691A1 (en) | 2016-05-26 | 2017-11-30 | Cellular Research, Inc. | Molecular label counting adjustment methods |
US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
EP4300099A3 (en) | 2016-09-26 | 2024-03-27 | Becton, Dickinson and Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
AU2017359047B2 (en) | 2016-11-08 | 2024-06-13 | Becton, Dickinson And Company | Methods for cell label classification |
CN109952612B (en) | 2016-11-08 | 2023-12-01 | 贝克顿迪金森公司 | Method for classifying expression profiles |
US10722880B2 (en) | 2017-01-13 | 2020-07-28 | Cellular Research, Inc. | Hydrophilic coating of fluidic channels |
CN110382708A (en) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | Selective amplification is carried out using blocking property oligonucleotides |
CA3059559A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
CN111433372B (en) | 2017-10-19 | 2024-01-26 | 生物辐射实验室股份有限公司 | Digital amplification assay with unusual and/or inverse variation of photoluminescence |
EP3728636A1 (en) | 2017-12-19 | 2020-10-28 | Becton, Dickinson and Company | Particles associated with oligonucleotides |
EP4234717A3 (en) | 2018-05-03 | 2023-11-01 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
EP3788170A1 (en) | 2018-05-03 | 2021-03-10 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
EP3861134A1 (en) | 2018-10-01 | 2021-08-11 | Becton, Dickinson and Company | Determining 5' transcript sequences |
JP2022506546A (en) | 2018-11-08 | 2022-01-17 | ベクトン・ディキンソン・アンド・カンパニー | Single-cell whole transcriptome analysis using random priming |
EP3894552A1 (en) | 2018-12-13 | 2021-10-20 | Becton, Dickinson and Company | Selective extension in single cell whole transcriptome analysis |
US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
US11661631B2 (en) | 2019-01-23 | 2023-05-30 | Becton, Dickinson And Company | Oligonucleotides associated with antibodies |
WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
US11939622B2 (en) | 2019-07-22 | 2024-03-26 | Becton, Dickinson And Company | Single cell chromatin immunoprecipitation sequencing assay |
CN114729350A (en) | 2019-11-08 | 2022-07-08 | 贝克顿迪金森公司 | Obtaining full-length V (D) J information for immunohistorian sequencing using random priming |
CN115244184A (en) | 2020-01-13 | 2022-10-25 | 贝克顿迪金森公司 | Methods and compositions for quantifying protein and RNA |
WO2021231779A1 (en) | 2020-05-14 | 2021-11-18 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
CN116635533A (en) | 2020-11-20 | 2023-08-22 | 贝克顿迪金森公司 | Profiling of high and low expressed proteins |
US11851221B2 (en) | 2022-04-21 | 2023-12-26 | Curium Us Llc | Systems and methods for producing a radioactive drug product using a dispensing unit |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1997029211A1 (en) * | 1996-02-09 | 1997-08-14 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs |
US6010850A (en) * | 1995-08-01 | 2000-01-04 | Yale University | Analysis of gene expression by display of 3'-end restriction fragments of cDNAs |
WO2001048247A2 (en) * | 1999-12-29 | 2001-07-05 | Arch Development Corporation | METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION |
WO2002006145A1 (en) * | 2000-07-13 | 2002-01-24 | Emsize Ab | Device and method for switching between materials |
WO2002008461A2 (en) * | 2000-07-21 | 2002-01-31 | Global Genomics Ab | A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS |
-
2003
- 2003-01-28 EP EP03702979A patent/EP1476569A2/en not_active Withdrawn
- 2003-01-28 AU AU2003206095A patent/AU2003206095A1/en not_active Abandoned
- 2003-01-28 CA CA002474864A patent/CA2474864A1/en not_active Abandoned
- 2003-01-28 JP JP2003564281A patent/JP2005515792A/en active Pending
- 2003-01-28 US US10/352,253 patent/US20030175908A1/en not_active Abandoned
- 2003-01-28 WO PCT/IB2003/000843 patent/WO2003064691A2/en not_active Application Discontinuation
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6010850A (en) * | 1995-08-01 | 2000-01-04 | Yale University | Analysis of gene expression by display of 3'-end restriction fragments of cDNAs |
WO1997029211A1 (en) * | 1996-02-09 | 1997-08-14 | The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services | RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs |
WO2001048247A2 (en) * | 1999-12-29 | 2001-07-05 | Arch Development Corporation | METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION |
WO2002006145A1 (en) * | 2000-07-13 | 2002-01-24 | Emsize Ab | Device and method for switching between materials |
WO2002008461A2 (en) * | 2000-07-21 | 2002-01-31 | Global Genomics Ab | A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS |
Non-Patent Citations (2)
Title |
---|
MIKHAIL MATZ ET AL: "Ordered differential display: a simple method for systematic comparison of gene expression profiles", NUCLEIC ACIDS RESEARCH, vol. 25, no. 12, 1997, pages 2541 - 2542, XP002253451 * |
SERGEY IVASHUTA ET AL: "The Coupling of Differential Display and AFLP Approaches for Nonradioactive mRNA Fingerprinting", MOLECULAR BIOTECHNOLOGY, vol. 12, 1999, pages 137 - 141, XP002253452 * |
Also Published As
Publication number | Publication date |
---|---|
US20030175908A1 (en) | 2003-09-18 |
JP2005515792A (en) | 2005-06-02 |
WO2003064691A2 (en) | 2003-08-07 |
AU2003206095A1 (en) | 2003-09-02 |
CA2474864A1 (en) | 2003-08-07 |
EP1476569A2 (en) | 2004-11-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2003064691A3 (en) | Methods and means for amplifying nucleic acid | |
WO2007086935A8 (en) | Methods of amplifying and sequencing nucleic acids | |
WO2005003375A3 (en) | Methods of amplifying and sequencing nucleic acids | |
WO2002052031A3 (en) | Nucleic acid amplification | |
WO2002101022A3 (en) | Method for rapid amplification of dna | |
WO2005098050A3 (en) | Base specific cleavage of methylation-specific amplification products in combination with mass analysis | |
IL131966A0 (en) | An in vitro method for construction of a dna library | |
CA2365135A1 (en) | Method for amplifying nucleic acid sequence | |
WO2008084405A3 (en) | Circular chromosome conformation capture (4c) | |
WO2003008624A3 (en) | Nucleic acid amplification using nicking agents | |
WO2006081284A3 (en) | Methods for quantitating small rna molecules | |
WO2005091971A3 (en) | Compositions for use in identification of alphaviruses | |
WO2001048237A3 (en) | Method for quickly detecting microbial dna/rna, kit therefor and the use of said method | |
WO2005097205A3 (en) | Dna virus microrna and methods for inhibiting same | |
AU7104300A (en) | Method for amplifying signal-flanking sequences from unknown genomic dna | |
WO2005083115A3 (en) | Method for the identification of sepsis | |
WO2006030455A8 (en) | Dna polymerases having strand displacement activity | |
NZ505062A (en) | Detection of fungal pathogens in grape plants using oligonucleotide primers | |
WO2007076143A3 (en) | Species-specific primer sets and identification of species-specific dna sequences using genome fragment enrichment | |
WO2000050564A3 (en) | Method for extracting dna from dried specimens | |
EP1741793A4 (en) | Method of hybridization | |
WO2002040126A3 (en) | Methods for identifying nucleotides at defined positions in target nucleic acids using fluorescence polarization | |
WO2005047462A3 (en) | Universal control for nucleic acid amplification | |
WO2007073523A3 (en) | MITIGATION OF Cot-1 DNA DISTORTION IN NUCLEIC ACID HYBRIDIZATION | |
WO2006137939A3 (en) | Compositions and methods for detecting group a streptococci |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2474864 Country of ref document: CA Ref document number: 2003564281 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2003702979 Country of ref document: EP |
|
WWP | Wipo information: published in national office |
Ref document number: 2003702979 Country of ref document: EP |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2003702979 Country of ref document: EP |