WO2003064691A3 - Methods and means for amplifying nucleic acid - Google Patents

Methods and means for amplifying nucleic acid Download PDF

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Publication number
WO2003064691A3
WO2003064691A3 PCT/IB2003/000843 IB0300843W WO03064691A3 WO 2003064691 A3 WO2003064691 A3 WO 2003064691A3 IB 0300843 W IB0300843 W IB 0300843W WO 03064691 A3 WO03064691 A3 WO 03064691A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
nucleic acid
oligonucleotides
pcr
electrophoresis
Prior art date
Application number
PCT/IB2003/000843
Other languages
French (fr)
Other versions
WO2003064691A2 (en
Inventor
Sten Linnarsson
Patrik Ernfors
Goran Bauren
Ats Metsis
Arno Pihlak
Andreas Montelius
Original Assignee
Global Genomics Ab
Sten Linnarsson
Patrik Ernfors
Goran Bauren
Ats Metsis
Arno Pihlak
Andreas Montelius
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Global Genomics Ab, Sten Linnarsson, Patrik Ernfors, Goran Bauren, Ats Metsis, Arno Pihlak, Andreas Montelius filed Critical Global Genomics Ab
Priority to AU2003206095A priority Critical patent/AU2003206095A1/en
Priority to JP2003564281A priority patent/JP2005515792A/en
Priority to EP03702979A priority patent/EP1476569A2/en
Priority to CA002474864A priority patent/CA2474864A1/en
Publication of WO2003064691A2 publication Critical patent/WO2003064691A2/en
Publication of WO2003064691A3 publication Critical patent/WO2003064691A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Methods of manipulation of nucleic acid, in particular amplification by means of the polymerase chain reaction (PCR), including use of oligonucleotides and combinations and kits comprising such oligonucleotides, also methods comprising use of nested PCR, allowing for improved results in methods wherein large numbers of nucleic acid fragments are manipulated by means of PCR and electrophoresis. Oligonucleotides are provided for use a size standards in electrophoresis, and internal controls allowing for calculation of relative amounts of material present. Improved results can be achieved in methods of profiling mRNA transcribed in a system under investigation.
PCT/IB2003/000843 2002-01-29 2003-01-28 Methods and means for amplifying nucleic acid WO2003064691A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2003206095A AU2003206095A1 (en) 2002-01-29 2003-01-28 Methods and means for amplifying nucleic acid
JP2003564281A JP2005515792A (en) 2002-01-29 2003-01-28 Methods and means for manipulating nucleic acids
EP03702979A EP1476569A2 (en) 2002-01-29 2003-01-28 Methods and means for manipulating nucleic acid
CA002474864A CA2474864A1 (en) 2002-01-29 2003-01-28 Methods and means for amplifying nucleic acid

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US35221502P 2002-01-29 2002-01-29
US60/352,215 2002-01-29

Publications (2)

Publication Number Publication Date
WO2003064691A2 WO2003064691A2 (en) 2003-08-07
WO2003064691A3 true WO2003064691A3 (en) 2003-11-27

Family

ID=27663061

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2003/000843 WO2003064691A2 (en) 2002-01-29 2003-01-28 Methods and means for amplifying nucleic acid

Country Status (6)

Country Link
US (1) US20030175908A1 (en)
EP (1) EP1476569A2 (en)
JP (1) JP2005515792A (en)
AU (1) AU2003206095A1 (en)
CA (1) CA2474864A1 (en)
WO (1) WO2003064691A2 (en)

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US7901882B2 (en) 2006-03-31 2011-03-08 Affymetrix, Inc. Analysis of methylation using nucleic acid arrays
WO2008123019A1 (en) * 2007-03-05 2008-10-16 Olympus Corporation Method of detecting change in gene and detection device
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
AU2012231098B2 (en) * 2011-03-18 2016-09-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
ES2904816T3 (en) * 2012-02-27 2022-04-06 Becton Dickinson Co Molecular counting compositions
US9970052B2 (en) 2012-08-23 2018-05-15 Bio-Rad Laboratories, Inc. Digital assays with a generic reporter
PL225633B1 (en) 2012-11-22 2017-05-31 Univ Jagielloński Method for identification of RNA viruses and the application of the method
KR102402446B1 (en) 2013-08-28 2022-05-30 벡톤 디킨슨 앤드 컴퍼니 Massively parallel single cell analysis
US9582877B2 (en) 2013-10-07 2017-02-28 Cellular Research, Inc. Methods and systems for digitally counting features on arrays
WO2016134078A1 (en) 2015-02-19 2016-08-25 Becton, Dickinson And Company High-throughput single-cell analysis combining proteomic and genomic information
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
EP3277843A2 (en) 2015-03-30 2018-02-07 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (en) 2015-04-23 2021-12-28 贝克顿迪金森公司 Methods and compositions for whole transcriptome amplification
WO2016196229A1 (en) 2015-06-01 2016-12-08 Cellular Research, Inc. Methods for rna quantification
EP3347465B1 (en) 2015-09-11 2019-06-26 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
WO2017192387A1 (en) 2016-05-02 2017-11-09 Cellular Research, Inc. Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP4300099A3 (en) 2016-09-26 2024-03-27 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
AU2017359047B2 (en) 2016-11-08 2024-06-13 Becton, Dickinson And Company Methods for cell label classification
CN109952612B (en) 2016-11-08 2023-12-01 贝克顿迪金森公司 Method for classifying expression profiles
US10722880B2 (en) 2017-01-13 2020-07-28 Cellular Research, Inc. Hydrophilic coating of fluidic channels
CN110382708A (en) 2017-02-01 2019-10-25 赛卢拉研究公司 Selective amplification is carried out using blocking property oligonucleotides
CA3059559A1 (en) 2017-06-05 2018-12-13 Becton, Dickinson And Company Sample indexing for single cells
CN111433372B (en) 2017-10-19 2024-01-26 生物辐射实验室股份有限公司 Digital amplification assay with unusual and/or inverse variation of photoluminescence
EP3728636A1 (en) 2017-12-19 2020-10-28 Becton, Dickinson and Company Particles associated with oligonucleotides
EP4234717A3 (en) 2018-05-03 2023-11-01 Becton, Dickinson and Company High throughput multiomics sample analysis
EP3788170A1 (en) 2018-05-03 2021-03-10 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3861134A1 (en) 2018-10-01 2021-08-11 Becton, Dickinson and Company Determining 5' transcript sequences
JP2022506546A (en) 2018-11-08 2022-01-17 ベクトン・ディキンソン・アンド・カンパニー Single-cell whole transcriptome analysis using random priming
EP3894552A1 (en) 2018-12-13 2021-10-20 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
US11371076B2 (en) 2019-01-16 2022-06-28 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
US11661631B2 (en) 2019-01-23 2023-05-30 Becton, Dickinson And Company Oligonucleotides associated with antibodies
WO2020214642A1 (en) 2019-04-19 2020-10-22 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN114729350A (en) 2019-11-08 2022-07-08 贝克顿迪金森公司 Obtaining full-length V (D) J information for immunohistorian sequencing using random priming
CN115244184A (en) 2020-01-13 2022-10-25 贝克顿迪金森公司 Methods and compositions for quantifying protein and RNA
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
CN116635533A (en) 2020-11-20 2023-08-22 贝克顿迪金森公司 Profiling of high and low expressed proteins
US11851221B2 (en) 2022-04-21 2023-12-26 Curium Us Llc Systems and methods for producing a radioactive drug product using a dispensing unit

Citations (5)

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US6010850A (en) * 1995-08-01 2000-01-04 Yale University Analysis of gene expression by display of 3'-end restriction fragments of cDNAs
WO2001048247A2 (en) * 1999-12-29 2001-07-05 Arch Development Corporation METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION
WO2002006145A1 (en) * 2000-07-13 2002-01-24 Emsize Ab Device and method for switching between materials
WO2002008461A2 (en) * 2000-07-21 2002-01-31 Global Genomics Ab A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6010850A (en) * 1995-08-01 2000-01-04 Yale University Analysis of gene expression by display of 3'-end restriction fragments of cDNAs
WO1997029211A1 (en) * 1996-02-09 1997-08-14 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services RESTRICTION DISPLAY (RD-PCR) OF DIFFERENTIALLY EXPRESSED mRNAs
WO2001048247A2 (en) * 1999-12-29 2001-07-05 Arch Development Corporation METHOD FOR GENERATION OF LONGER cDNA FRAGMENTS FROM SAGE TAGS FOR GENE IDENTIFICATION
WO2002006145A1 (en) * 2000-07-13 2002-01-24 Emsize Ab Device and method for switching between materials
WO2002008461A2 (en) * 2000-07-21 2002-01-31 Global Genomics Ab A METHOD AND AN ALGORITHM FOR mRNA EXPRESSION ANALYSIS

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
MIKHAIL MATZ ET AL: "Ordered differential display: a simple method for systematic comparison of gene expression profiles", NUCLEIC ACIDS RESEARCH, vol. 25, no. 12, 1997, pages 2541 - 2542, XP002253451 *
SERGEY IVASHUTA ET AL: "The Coupling of Differential Display and AFLP Approaches for Nonradioactive mRNA Fingerprinting", MOLECULAR BIOTECHNOLOGY, vol. 12, 1999, pages 137 - 141, XP002253452 *

Also Published As

Publication number Publication date
US20030175908A1 (en) 2003-09-18
JP2005515792A (en) 2005-06-02
WO2003064691A2 (en) 2003-08-07
AU2003206095A1 (en) 2003-09-02
CA2474864A1 (en) 2003-08-07
EP1476569A2 (en) 2004-11-17

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