WO2003063591A1

WO2003063591A1 - An agent for the inhibition and control of plant viruses

Info

Publication number: WO2003063591A1
Application number: PCT/CN2001/001641
Authority: WO
Inventors: Qinfu Zhang; Derong An; Chi Ma
Original assignee: Xi An Hi-Level Chemical Industry Co., Ltd.
Priority date: 2001-12-25
Filing date: 2001-12-25
Publication date: 2003-08-07

Abstract

An agent for the inhibition and control of plant viruses comprises 0.02-2.0wt % of ribavirin, 0.5-15.0wt % of copper sulfate, 1.0-25.0wt % of zinc sulfate, and water as solvent. The preferred composition comprises 0.08-0.12wt % of ribavirin, 1.2-1.3wt % of copper sulfate, 2.0-3.0wt % of zinc sulfate, and water as solvent. During pilot period, the agent is tested at more than twenty demonstration plots on plants such as tobaccos, fruit trees, vegetables, and so on. The results show that the product can effectively control various plant virosis caused by cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV), with the controlling rate of 80 %, and has no toxic side effects. Therefore the agent which has systemic and transmissive activity is available to inhibit and control plant viruses.

Description

TECHNICAL FIELD

The invention relates to pesticides for controlling plant viruses, in particular to a plant virus inhibitory control agent.

Background technique

In recent years, due to the obvious changes in the agricultural environment such as three-dimensional agriculture and greenhouses, coupled with the greenhouse effect, the temperature has increased and the drought has been severe, which has caused more opportunities for plant virus infection. Plant viruses frequently occur, and they involve a wide area and many types of crops. Agricultural production caused great harm. A plant virus is an absolutely endoparasite non-cellular organism that has only nucleic acids and coat proteins. After invading the plant, it completely depends on the place, energy and synthetic system provided by the parasitic cells for self-replication and proliferation. The serious harm to the plants is the consumption of nutrients, disrupting the growth balance and reducing the immunity of the plants. The susceptible plants show symptoms such as mosaic, dwarfing, shrinking, deformity, bushing, and decline. Eventually it leads to a decline in material output and quality, which seriously harms agricultural production. Plant virus is called "plant cancer" because of its special survival mode and difficult control. At present, there are only a few pesticide varieties for the prevention and treatment of viral diseases in China. The representative varieties such as Zhibingling (produced by Shandong Penglai Pesticide Factory) and virus A (produced by Qiqihar Pesticide Factory), etc., not only have small output, but also have only control effect About 40%, it is difficult to meet the actual needs of agricultural production. There are very few such pesticides abroad. At present, ribavirin has been widely used as a viral inhibitor to treat diseases such as human viral influenza, and also for livestock viral diseases. However, no reports have been reported for plant virus control. Plant viruses and animal viruses are: very different. The inventors have been engaged in plant virus induced resistance, surface passivation, and virus replication inhibitor tests for many years, and have accumulated a large amount of relevant data and materials. Studies have shown that ribavirin also inhibits and controls plant viruses; copper sulfate, zinc sulfate, and the like have virus surface-active deactivators. But any single component virus inhibitor ribavirin or virus surface active deactivator copper sulfate, sulfuric acid Zinc, etc., are not effective in controlling plant virus diseases. For example, if triazole riboside 2.0% concentrated solution is used alone, its control effect is lower than 40%, and there is almost no control effect. Although copper sulfate and zinc sulfate are widely used as pesticides and fungicides, they have no killing effect on viruses. The production cost of ribavirin is high, and the excessively high concentration is very expensive, which is difficult for farmers to accept. To date, no reports have been reported on the use of ribavirin to control plant viruses. Disclosure of invention

The purpose of the present invention is to overcome the shortcomings of the existing single ribavirin single component to prevent and control plant virus diseases, and to improve on this basis, so as to provide a cheap and highly effective plant virus suppression control agent.

Embodiment of the invention

The plant virus suppressing and controlling agent of the present invention contains the following components (the sum of the weight percentages of the components is 100%):

a) Ribavirin 0.02-2.0%;

b) Copper sulfate 0.5-15.0%

c) zinc sulfate 1.0 ~ 25.0%;

d) The rest are water solvents.

The preferred composition of the virus inhibitory agent of the present invention is:

a) Ribavirin 0.08-0.12%;

b) υ Copper acid 1.2 ~ 1.3%

c) zinc combate 2.0 ~ 3.0%;

d) it is a water solvent.

Various additives such as penetrant, emulsifier or surfactant can be added to the solution to improve its performance. These auxiliaries are commonly used materials, which are easy for ordinary professional technicians to understand and not to be confused.

The method for using the above-mentioned preferred component virus inhibitory agent can be diluted by water to 400-600 times, and sprayed on the plant surface. It is best to dilute to 500 times with water. The application frequency is 1 to 3 times, and the effect is better in the early stage of onset. The virus suppressing and controlling agent can be used as a plant virus disease controlling agent caused by cucumber mosaic virus (CMV) or tobacco mosaic virus (TMV) and other viruses in vegetables, grains, tobacco or fruit trees. It has significant preventive effects on symptoms such as mosaic disease, leaflet disease, yellow leaf disease, fern leaf disease, downy mildew, dwarf disease, sarcoidosis, shrinkage, rugosity, and decline. Especially for vegetable, tobacco, pepper, melon and fruit crops caused by cucumber mosaic virus (CMV), tobacco mosaic virus (TMV) caused by plant virus disease, the control effect reached 80%.

The action mechanism of the control agent is as follows: In the above composition, ribavirin is a plant virus inhibitor, and copper sulfate and zinc sulfate are plant virus passivating agents, which passivate the surface activity of the virus. For example, only one component of ribavirin can inhibit and prevent plant viruses. However, when used together with copper sulfate and zinc sulfate, the inhibitory effect of ribavirin was significantly enhanced, and at the same time, copper sulfate and zinc sulfate showed a strong virus surface active passivation. Therefore, the combination of the three ingredients has a significant synergistic effect, which can achieve a control effect of more than 80%. This is far from being achieved by any single component.

The present invention is a highly effective plant virus inhibitory control agent developed on the basis of plant virus replication inhibitors, which integrates inactivated virus activity, inhibits virus reproduction, induces and improves plant resistance. By inactivating the virus activity and inhibiting the virus from multiplying in the plant, it achieves the purposes of controlling diseases, increasing crop yield and improving crop quality. The preparation has a strong systemic action and conduction activity, and has a control effect on plant diseases caused by cucumber yellow leaf disease (CMV), tobacco mosaic disease (TMV) and other viruses of various plants by more than 80%. This preparation has been tested by the country's designated toxicity testing unit. LD50> 10000, it is "actually non-toxic", non-toxic and pollution-free, and no pollution to the environment.

This research provides theoretical basis for the plant's antiviral mechanism-the relationship between plants and viruses from both the basis of biochemical resistance and application. The plant virus suppressor was extracted and identified for the first time in China, and the process of inhibition was clarified. Plant virus control provides a new approach. And through a large number of tests, it has been compounded with various combinations of azoles and other deactivators and inhibitors more than 500 times. A low-cost three-principle screening and development of this plant virus inhibitor.

Plant surfaces are usually oily and have poor wetting effects. Additives such as penetration should be added to the formulation to facilitate the formation of a continuous medicinal film on the foliage of the crop.

For ease of transportation and storage, this formulation is also in the form of a wettable powder. Among them, the ratio of three active ingredients of ribavirin, copper sulfate and zinc sulfate is basically the same as that of the aforementioned water emulsion. Temporarily add water at the farm site to make a liquid spray. Ultimately, it should meet the optimal ratio. Fillers such as bentonite and diatomaceous earth can be added to the wettable powder. Both of these preparation forms are within the protection scope of the present invention.

The production process of the plant virus control agent is as follows:

First, weigh each amount of ribavirin, copper sulfate, and sulfuric acid according to the ratio: then add solvent to dissolve in the water, and carry out the mixing reaction. At the same time, add an appropriate amount of emulsifier and penetrant and other auxiliary agents. Stir well. Ready. Each component should reach the content described in claim 2. When using, spray according to the conventional pesticide spray method. The wettable powder is used after being dissolved in water and diluted to the specified range.

Best Mode for Carrying Out the Invention

Several specific embodiments of the present invention are described in detail below to explain the present invention.

Preparation Example

Example 1:

Ribavirin 0.10%, copper sulfate (added with CuS0 ₄ 5H ₂ 0) 1.25%, zinc sulfate (added with ZnS0 ₄ · 7Η ₂ 0) 2.5%, appropriate amount of auxiliary. The floatation agent formulated according to the above ratio has a control effect of more than 80%, which is the best ratio. Because the total content of the three active ingredients is 3.85%, the pesticide of this kind is called "3.85% Virus Bic"-the commercial name of this product.

Example 2:

Ribavirin 0.02%, copper sulfate (added with CuS0 ₄ 5H ₂ 0) 0.50%, zinc sulfate (added with ZnS0 ₄ · 7H ₂ 0) 1.0%. The control effect is 47%, and the content is lower than this ratio, which has almost no control effect. Example 3:

Ribavirin 2.0%, copper sulfate (added with CuS0 ₄ · 5Η ₂ 0) 15.0%, zinc sulfate (added with ZnS0 ₄ 7H ₂ 0) 25.0%. The control effect is 58%, which means that the content is too high and it will affect the control effect. If it is higher than this ratio, it may cause phytotoxicity to plants.

In the pilot phase of this product, plants such as tobacco, fruit trees, and vegetables were verified in more than 20 demonstration areas. The results show that this product has a high control rate on cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) of various plants, and has no negative effects. It is an ideal plant virus inhibitory control agent with systemic and conductive activity.

Biological test example

The experiment was conducted in a field plot, with 4 repetitions arranged randomly. The test pesticide was prepared by diluting the various formulations of the present invention to 400-fold, 500-fold, and 600-fold liquids with water; using conventional virus disease control agents virus, Zhikangling II and Bacterial Clear corresponding dilutions and water as comparison. Foliar spray is applied to the plants 1-3 times, and then the crops are classified according to the following criteria:

Grade 0 plants grow normally and without symptoms;

Class 1 light flower leaves, leaves spread out, not dwarfed;

Class 3 flowers and leaves are obvious, leaves are shrunken and twisted;

Grade 5 leaves are deformed and the plant is slightly dwarfed;

Grade 7 The leaves are deformed and the plants are severely dwarfed.

Calculated according to the following formula:

4 («/, ∑ (the number of diseased plants at each level X the value of this level) _1ΠΛ disease index (%) = number of investigated plants χ ₇ ^Xl0 ° control effect (%) = [1-. _X ^U ] χΐθθ

"to

among them:

Number of diseased plants at each level-according to the above classification, Number of plants

The value of this level-the disease grade value, such as the value of level 0 is 0, the value of level 3 is 3; the total number of plants surveyed in the test area and the control area;

7——the last level value;

CKo—a disease index before administration in a control area;

P _t0- the disease index before application in the test area;

CK! —The condition index after application in the control area;

Pti——Illness index after application in the test area;

Experimental results:

The plant virus control agent of the present invention, for example, takes "3.85%% virus bike" in Example 1, and has the following obvious effects:

1. Through field experiments, the 500-fold solution of this agent has a control effect on tomato mosaic virus (CMV) of 83.6%, which is higher than that of existing similar agents, such as virus A 67.1

%.

2. The 500-fold solution of this agent has a control effect on corn virus disease (CMV) of 84.7%, the disease index in the treatment area is 2.65, and the control area is 32.30.

3. The 500-fold solution of this agent has a control effect on tobacco virus disease (TMV) of 81.45%. It is much higher than 45.45% of Zhejinling II emulsion.

In the pilot phase of this product, plants such as tobacco, fruit trees, and vegetables were verified in more than 20 demonstration areas. The results show that the product has a control effect on plant virus diseases caused by cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) of various plants up to 80%, and has no negative effects. It is an ideal systemic and conductive activity. Plant virus inhibitor.

4. Pesticide safety toxicology test report confirms low toxicity and is non-irritating to eyes and skin.

Industrial '1 "Health

The preparation is non-toxic and non-polluting, has low production cost, and has good effects in inhibiting and treating diseases. It is an urgently needed pesticide product in agricultural production.

Claims

1. A plant virus inhibitory control agent, characterized by containing the following ingredients (the sum of the weight percentages of the components is 100%):

a) Ribavirin 0.02-2.0%;

b) Copper sulfate 0.5 ~ 15.0%

c) zinc sulfate 1.0-25.0%;

d) it is a water solvent.

2. The virus inhibitory agent according to claim 1, wherein the composition is: a) ribavirin 0.08 to 0.12%;

b) Copper sulfate 1.2 ~ 1.3%

c) zinc sulfate 2.0 ~ 3.0%;

d) The rest are water solvents.

3. The virus suppressing and controlling agent according to claim 1 or 2, wherein a penetrant, an emulsifier or a surfactant is added to the solution.

4. The method of using a virus suppression control agent according to claim 1, 2 or 3, characterized in that it is diluted by adding water to 400 to 600 times, and spraying the plant surface.

5. The method of using a virus suppression control agent according to claim 4, characterized in that it is diluted 500 times with water and sprayed on the plant surface.

6. The virus suppressing and controlling agent according to claim 1, 2 or 3 as a plant virus disease controlling agent caused by cucumber mosaic virus (CMV) or tobacco mosaic virus (TMV) in vegetables, food, tobacco or fruit trees.