WO2003061629A2 - Dna dosage forms - Google Patents
Dna dosage forms Download PDFInfo
- Publication number
- WO2003061629A2 WO2003061629A2 PCT/GB2003/000336 GB0300336W WO03061629A2 WO 2003061629 A2 WO2003061629 A2 WO 2003061629A2 GB 0300336 W GB0300336 W GB 0300336W WO 03061629 A2 WO03061629 A2 WO 03061629A2
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- dna
- pharmaceutical agent
- dosage form
- agent dosage
- dna pharmaceutical
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
- A61K9/0021—Intradermal administration, e.g. through microneedle arrays, needleless injectors
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/20—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing sulfur, e.g. dimethyl sulfoxide [DMSO], docusate, sodium lauryl sulfate or aminosulfonic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
- A61K9/167—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface
- A61K9/1676—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction with an outer layer or coating comprising drug; with chemically bound drugs or non-active substances on their surface having a drug-free core with discrete complete coating layer containing drug
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/16—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing nitrogen, e.g. nitro-, nitroso-, azo-compounds, nitriles, cyanates
- A61K47/18—Amines; Amides; Ureas; Quaternary ammonium compounds; Amino acids; Oligopeptides having up to five amino acids
- A61K47/183—Amino acids, e.g. glycine, EDTA or aspartame
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/141—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers
- A61K9/145—Intimate drug-carrier mixtures characterised by the carrier, e.g. ordered mixtures, adsorbates, solid solutions, eutectica, co-dried, co-solubilised, co-kneaded, co-milled, co-ground products, co-precipitates, co-evaporates, co-extrudates, co-melts; Drug nanoparticles with adsorbed surface modifiers with organic compounds
Definitions
- the present invention relates to DNA pharmaceutical formulations, and preferably those formulations that are suitable for ballistic delivery into the skin of the human body.
- the present invention provides a novel DNA pharmaceutical agent dosage form, having a dense core element which is coated with an amorphous solid reservoir medium containing the DNA pharmaceutical agent in solid solution or suspension within it.
- the dense core element is preferably a small metal bead suitable for ballistic delivery of the agent into a cell, commonly such beads are roughly spherical gold or tungsten microbeads of an average particle size in the range of between 0.5 to 10 micrometers in diameter.
- the solid pharmaceutical reservoir medium coating the beads is a polyol, preferably being a polyol in an amorphous state.
- the polyol is a carbohydrate such as trehalose or sucrose.
- the solid pharmaceutical reservoir medium may further comprise a stabilising agent that inhibits the degradative effects of free radicals, such as a free radical scavenger or a metal ion chelator.
- the DNA pharmaceutical formulations of the present invention are storage stable, in that the DNA is stabilised in its supercoiled form, and only substantially release the DNA after administration to the skin. Furthermore, vaccine delivery devices for the administration of the DNAvaccines into the skin are provided, methods of their manufacture, and their use in medicine.
- the skin represents a significant barrier to external agents.
- a summary of human skin is provided in Dorland's Illustrated Medical Dictionary, 28 th Edition. Starting from the external layers, working inwards, the skin comprises the epithelium consisting of the stratum corneum and the viable epithelium, and underlying the epithelium is the dermis.
- the viable epithelium consists of four layers: Stratum corneum, Stratum lucidium, Stratum granulosum, Stratum spinosum, and Stratum basale.
- the epithelium (including all five layers) is the outermost non-vascular layer of the skin, and varies between 0.07 and 0.12 mm thick (70-120 ⁇ m).
- the epithelium is populated with keratinocytes, a cell that produces keratin and constitutes 95% of the dedicated epidermal cells.
- the other 5% of cells are melanocytes.
- the underlying dermis is normally found within a range of 0.3 to about 3 mm beneath the surface of the stratum corneum, and contains sweat glands, hair follicles, nerve endings and blood vessels.
- the stratum corneum dominates the skin permeability barrier and consists of a few dozen horny, keratinised epithelium layers.
- the narrow interstices between the dead or dying keratinocytes in this region are filled with crystalline lipid multilamellae. These efficiently seal the interstices between the skin or body interior and the surroundings by providing a hydrophobic barrier to entry by hydrophilic molecules.
- the stratum corneum being in the range of 30-70 ⁇ m thick.
- Langerhans cells are found throughout the basal granular layer of the viable epithelium (stratum spinosum and stratum granulosum, (Small Animal Dermatology - Third Edition, Muller - Kirk - Scott, Ed: Saunders (1983)) and are considered to play an important role in the immune system's initial defence against invading organisms. This layer of the skin therefore represents a suitable target zone for certain types of vaccine.
- DNA vaccines usually consist of a bacterial plasmid vector into which is inserted a strong viral promoter, the gene of interest which encodes for an antigenic peptide and a polyadenylation/transcriptional termination sequences.
- the gene of interest may encode a full protein or simply an antigenic peptide sequence relating to the pathogen, tumour or other agent which is intended to be protected against.
- the plasmid can be grown in bacteria, such as for example E.coli and then isolated and prepared in an appropriate medium, depending upon the intended route of administration, before being administered to the host. Following administration the host cells produce the plasmid encoded protein or peptide.
- the plasmid vectors are generally made without an origin of replication which is functional in eukaryotic cells, in order to prevent plasmid replication in the mammalian host and integration within chromosomal DNA of the animal concerned.
- Information in relation to DNA vaccination is provided in Donnelly et al "DNA vaccines" Ann. Rev Immunol. 1997 15: 617-648, the disclosure of which is included herein in its entirety by way of reference.
- Plasmid based delivery of genes, particularly for immunisation or gene therapy purposes is known. For example, administration of naked DNA by injection into mouse muscle is outlined in WO90/11092.
- Johnston et al WO 91/07487 describe methods of transferring a gene to veterbrate cells, by the use of microbeads onto which a polynucleotide encoding a gene of interest has been precipitated, and accelerating the DNA/microbeads such that they penetrate the target cell.
- Devices for administration of gold or tungsten beads coated with DNA into cells of the skin are described in US 5,630,796; WO 96/04947; WO 96/12513; WO 96/20022; WO 97/34652; WO 97/48485; WO 99/01168; WO 99/01169.
- Methods of vaccination using crystalline forms of ballistically delivered pharmaceutical agent are described in WO 99/27961.
- the present invention provides improved DNA dosage forms for use needleless ballistic delivery devices such as those described in the above publications.
- the formulations wherein DNA is precipitated onto gold beads as described in the art have the problem that it is difficult to co-formulate the DNA with additional agents/excipients.
- the present invention provides a method of co-formulation of additional agents.
- Solid dosage forms comprising a pharmaceutical agent (including DNA plasmids) and a stabilising polyol (such as a sugar) wherein the dosage forms are in the form of ballistically delivered powders are described in WO 96/03978.
- the stabilisation of agents in amorphous sugar glasses has been described in US 5,098,893.
- Sugars used in pharmaceutical formulations can be either crystalline or amorphous.
- Amorphous solids are distinguished from ciytalline by their lack of three- dimensional long-range order found in crystalline materials. Amorphous solids are similar to liquids at a molecular level wherein the molecules are randomly arranged.
- Amorphous sugars impart stability to pharmaceutical formulations when stored at temperatures below the glass transition temperature. Amorphous sugars exhibit a property in which there is a change in the mobility of the molecules in the sugar matrix below a temperature called the glass transition temperature. Below this temperature (Tg), amorphous sugars exist in a glassy state and above this temperature in a rubbery state.
- DNA pharmaceutical agents it is desirable for DNA pharmaceutical agents to be delivered in a supercoiled form.
- Supercoiled DNA in liquid pharmaceutical preparations are known to degrade over time resulting in the loss of the supercoiled structure and associated formation of open circle or linear DNA structures (Evans et al, 2000, Journal of Pharmaceutical Sciences, 89(1), 76-87; WO 97/40839).
- One mechanism by which this chain scission reaction may occur is oxidation of the DNA by free hydroxyl radicals produced from dissolved oxygen in the DNA solutions, a process that is catalysed by metal ions.
- the free radical formation reaction may be catalysed by several transition metal ions, the most common of which, however, are iron and copper ions (Fe , Fe , Cu or Cu ; Evans et al. supra).
- the present invention overcomes these problems and provides a DNA delivery dose which is capable of administering and releasing the DNA agents efficiently into the skin, with or without additional excipients, and also in which the DNA is stabilised in its supercoiled form. DESCRIPTION OF FIGURES
- FIG l ⁇ VACl.ova
- FIG 2 shows a graphical plot of the percentage of supercoiled plasmid, (%ccc), both monomeric, (%cccmon), and dimeric, (%cccdim), plasmid forms; after coating and lyophilization onto sowing needles and storage at 37°C.
- the plasmid formulations used contain varying amounts of sugars: FIG 2A: 5% Sucrose, FIG 2B: 10% Sucrose, FIG 2C: 17.5% Sucrose, FIG 2D: 40% Sucrose, FIG 2E: 40% Trehalose, FIG 2F: 40% Glucose.
- FIG 3 shows differential scanning calorimetry, (DSC), data, for plasmid DNA, (lOmg/ml), formulations in 40% sucrose.
- FIG 4 shows polarized light microscopy data, for plasmid DNA, (1 Omg/ml), formulations in 40% sucrose.
- Fig 4A formulations also contain: lOOmM TrisHCl pH8.0, lmM EDTA, lOmM methionine and 2.9% ethanol, Fig4C: only contains 40% sucrose and Fig 4D: shows crystals of the excipients described in the formulation shown in Fig 4 A.
- 1AM, 2AM & 3 AM represent a 24 hour lyophilization cycle, whereas 1ST, 2ST & 3ST represent a 1 hour lyophilization cycle.
- FIG 5 shows polarized light microscopy data, for lyophilisized plasmid DNA, (1 Omg/ml), formulations in sugars and polyols, which also contain: lOOmM TrisHCl ⁇ H8.0, lmM EDTA, lOmM methionine and 2.9% ethanol.
- Fig 5A sample 1: 40% w/v ficoll, sample 2: 20% w/v dextran, sample 3: 40% w/v sucrose, sample 4: 20% w/v maltotriose.
- Fig 5B sample 5: 20% w/v lactose, sample 6: 30% w/v maltose, sample 7: 40% w/v glucose, sample 8: 40% w/v trehalose.
- FIG 6 shows the stability of supercoiled DNA plasmid coated onto gold beads and stored for 1 week at 25°C.
- FIG 7 shows the stability of supercoiled DNA plasmid coated onto gold beads and stored for 3 weeks at 25°C.
- the present invention provides a novel DNA pharmaceutical agent dosage form, having a dense core element which is coated with a solid reservoir medium containing the DNA pharmaceutical agent.
- DNA vaccine dosage forms are a preferred aspect of the present invention.
- the agent to be delivered is a polynucleotide that encodes an antigen or antigens derivable from a pathogen such as micro-organisms or viruses, or may be a self antigen in the case of a cancer vaccine or other self antigen.
- the DNA component of the present invention may be linear or open circular or supercoiled plasmid DNA, but may in a related form of the present invention the DNA maybe in the form of a live attenuated bacterial or viral vector.
- Certain embodiments of the device described herein also have the significant advantage of being stored at room temperature thus reducing logistic costs and releasing valuable refrigerator space for other products.
- the solid amorphous reservoir medium is preferably a polyol that fulfils the function required for the present invention.
- the reservoir must be capable of adhering to the microbead to a sufficient extent that the reservoir remains physically stable and attached during prolonged storage, and also remains substantially intact during the administration procedure when the coated microbead is projected through the stratum corneum.
- the reservoir must also be capable of holding or containing a suspension or solution of agent to be delivered in any dry or partially dry form, which is released into the skin during biodegradation of the reservoir medium.
- Biodegradation of the medium in the sense of the present invention means that the reservoir medium changes state, such that changes from its non-releasing to its releasing states whereby the agent enters into the skin.
- the release of the active agent may involve one or more physical and/or chemical processes such as hydration, diffusion, phase transition, crystallisation, dissolution, enzymatic reaction and/or chemical reaction.
- biodegradation can be induced by one or more of the following: water, body fluids, humidity, body temperature, enzymes, catalysts and/or reactants.
- the change of the reservoir medium may therefore be induced by hydration, and warming associated with the higher humidity and temperature of the skin.
- the reservoir medium may then degrade by dissolution and/or swelling and/or change phase (crystalline or amorphous), thereby disintegrating or merely increase the permeation of the medium.
- the medium dissolves, and is metabolised or expelled or excreted from the body, but the reservoir may alternatively remain attached to microbead which maybe expelled from the body by several mechanisms including sloughing off of dead skin cells during normal skin replacement. Release of the agent by dissolution of the reservoir medium is preferred.
- the solid reservoir medium is a polyol (such as those described in WO 96/03978).
- Suitable polyol reservoir media include carbohydrates (such as sugars), polysaccharides, substituted polyols such as hydrophobically derivatised carbohydrates, amino acids, biodegradable polymers or co-polymers such as poly(hydroxy acid)s, polyahhydrides, poly(ortho)esters, polyurethanes, poly(butyric acid)s, poly(valeric acid)s, and ⁇ oly(lactide-co-caprolactone)s, or polylactide co- glycolide.
- the solid reservoir may be in an amorphous or crystalline state and may also be partially amorphous and partially crystalline.
- the reservoir is in an amorphous state. More preferably still is that the amorphous reservoir is in the form of a glass (US 5,098,893). Most preferably the reservoir is a sugar glass.
- a glass reservoir may have any glass transition temperature, but preferably it has a glass transition temperature that both stabilises the pharmaceutical agent during storage and also facilitates rapid release of the agent after insertion of the reservoir into the skin. Accordingly, the glass transition temperature is greater than 30-40°C, but most preferably is around body temperature (such as, but not limited to 40-50°C).
- Particularly preferred reservoir media are those that stabilise the agent to be delivered over the period of storage.
- antigen or agent dissolved or dispersed in a polyol glass or simply dried in a polyol are storage stable over prolonged periods of time (US 5,098,893, US 6,071,428; WO 98/16205; WO 96/05809; WO 96/03978; US 4,891,319; US 5,621,094; WO 96/33744).
- Such polyols form the preferred set of reservoir media.
- Preferred polyols include sugars, including mono, di, tri, or oligo saccharides and their corresponding sugar alcohols.
- Suitable sugars for use in the present invention are well known in the art and include, trehalose, sucrose, lactose, fructose, galactose, glucose, mannose, maltulose, iso-maltulose and lactulose, maltose, or dextrose and sugar alcohols of the aforementioned such as mannitol, lactitol and maltitol.
- Sucrose, Glucose, Lactose, Raffinose and Trehalose are preferred.
- the reservoir mediums of the present invention may preferably further contain a stabilising agent that inhibits the degradative effects of free radicals.
- Preferred stabilising agents include stabilising metal ion chelating agents, such preferred metal ion chelating agents include inositol hexaphosphate, tripolyphosphate, succinic and malic acid, ethylenediamine tetraacetic acid (EDTA), tromethamine (TRIS), Desferal, diethylenetriaminepentaacetic acid (DTP A) and ethylenediamindihydroxyphenylacetic acid (EDDHA).
- Other preferred stabilising agents are non-reducing free radical scavengers, and preferably such as agents are ethanol, methionine or glutathione.
- the preferred solid reservoir media in the devices of the present invention contain a metal ion chelating agent or a non-reducing free radical scavenger. Most preferably the solid reservoir media in the devices of the present invention contain both a metal ion chelating agent and a non-reducing free radical scavenger.
- the amounts of the stabilising agents may be determined by the man skilled in the art, but generally are in the range of 0.1-lOmM for the metal ion chelators, Ethanol is present in an amount up to about 5% (v/v), methionine is present at about 0.1 to lOOmM and Glutathione is present at about 0.1 to 10% (v/v).
- Preferred combinations of stabilising agents are (a) Phosphate buffered ethanol solution in combination with methionine or EDTA, (b) Tris buffered EDTA in combination with methionine or ethanol (or combinations of methionine and ethanol).
- Particularly preferred formulations which may be combined with DNA and coated onto the dense core elements to form solid dosage forms of the present invention contain polyols (preferably sucrose or trehalose) dissolved in demetalated water or Phosphate or Tris based buffers and further comprising either: A. lOmM methionine and 2.9% ethanol, or
- the DNA is stored and handled in these stabilising agents prior to final formulation with the sugar.
- further steps may be taken to enhance the stability of the DNA in the solid vaccines.
- the formulations maybe made using solutions which themselves were demetalated before use (for example by using commercially available demetalating resin such as Chelex 100 from Biorad) and/or the formulation may be finalised in a high pH (such as pH 8-10).
- the DNA is in the form of a supercoiled plasmid.
- a supercoiled plasmid is stabiUsed so that upon release, it largely remains in its supercoiled form, and preferably in its monomeric supercoiled form.
- Plasmid DNA stability can be defined in a number of ways and can be a relative phenomenon determined by the conditions of storage such as pH, humidity and temperature. For storage in the presence of iron ions on the coated reservoir, preferably >50% of plasmid remains supercoiled, (ccc, covalently closed circular), upon storage for 3 months at 4°C.
- >60% of plasmid remains ccc and more preferably, under these storage conditions, >90% of plasmid remains ccc for 3 months at 4°C.
- the stability of plasmid DNA would be preferably >60% and more preferably 80% and most preferably >90% ccc after 3 months storage at 4°C. More preferably, under these storage conditions, >90% of plasmid remains ccc for 1 year at 4°C, and more preferably >90% of plasmid remains ccc for 2 years at 4°C. Most preferably the above DNA stability is achieved under these conditions over the same time periods at 25°C.
- the DNA within a the solid reservoir medium is preferably stabilised in its supercoiled (ccc) form during accelerated stability studies, and most preferably the DNA is stabilised in its monomeric ccc form.
- ccc supercoiled
- An example of an acellarated stability study is where dry coated needles are maintained at 37°C for 4 weeks followed by analysis of the DNA structure over time.
- preferably greater than 50% of the DNA remains in its ccc form, more preferably greater than 60% remains in its ccc form, more preferably greater than 70% remains in its ccc form, more preferably greater than 80% remains in its ccc form and most preferably greater than 90% remains in its ccc form.
- the ratio of monomeric:dimeric ccc DNA is about 1 (such as within the range of 0.8-1.2, or more preferably within the range of 0.9-1.1 and most preferably within the range of 0.95- 1.5), or greater than 1.
- the ratio of monomeric:dimeric ccc can be measured by image intensity analysis after agarose gel electrophoresis (in the absence of any intercalating agents) and EtBr staining, using commercially available software such as Labworks 4.0 running on a UVP Bioimaging system.
- the solid reservoir medium coats the core elements in a manner that the resultant formulation is suitable for administration by ballistic delivery devices. Accordingly each core element may be fully or partially covered by the reservoir, or a plurality of elements may be trapped within a matrix of solid reservoir.
- a large quantity of reservoir encompassing a large number of core elements may be ground into smaller particles which are suitable for administration by ballistic delivery devices.
- phase change inhibitors which may be added to prevent phase change of the coating during procesing or storage or inhibitors to prevent deleterious chemical reactions during processing or storage such Maillard reaction inhibitors like amino acids.
- the solid dosage forms of the present invention are used in ballistic transfection of skin cells using devices that entrain the DNA coated particles in a gas flow.
- the particles pass through the stratum corneum and enter into a cell where the DNA is released and expressed by the host cell. Alternatively, the particle enters the extracellular space and releases the DNA therein.
- the core elements that are suitable for use in the present invention are those that are suitable for this purpose.
- the core elements impart upon the final dosage form sufficient strength and momentum to pierce the stratum corneum in any given ballistic delivery device. It is preferred that the core elements have sufficient density to impart sufficient momentum to the DNA coated particles, suitable dense cores have been found to be gold or tungsten microbeads.
- the size of the core elements is preferably that which imparts sufficient mass to give the required momentum to the DNA coated particles, whilst not being too large such that the skin cells suffer too much damage.
- Suitable core element particle sizes are those that when coated form particles of a mean diameter in the range of 0.5 to 100 ⁇ m, preferably between 1 to 50 ⁇ m, more preferably between 1 to lO ⁇ m, and most preferably around 2 ⁇ m in diameter.
- the core elements are roughly spherical, although non-regular forms may be used. Most preferably the core elements are gold or tungsten microbeads.
- the present invention claims that an amorphous sugar when present with metal particles and DNA will impart long-term stability to the formulation. Other excipients like surfactants and buffers may be included in the formulation.
- Spray drying is a dehydration process that utilizes heat from a hot gas stream (usually air) to evaporate dispersed droplets created by atomization of a continuous liquid feed. Resulting powder products dry within a few seconds into fine particles.
- the feasibility of spray drying for generating therapeutic protein powders has been amply demonstrated ((Broadhead, J., Rouan, S.K.E., Hau, I., and Rhodes, C.T. 1994. J. Pharm. Pharmacol. 46: 458-467.; Mumenthaler, M., Hsu, C.C., and Pearlman, R. 1994. Pharm. Res. 11 : 12-20)).
- the formulated DNA, gold particles and sugar solution will be fed into a spray dryer with a typical inlet temperature in the range of 50 to 150C typically at a flow rate between 0.1 and 10 mL/min.
- the resulting powder is dry and is collected from the collection chamber.
- Spray freeze-drying is a process in which the solution containing the DNA, gold particles and sugars is sprayed onto trays containing dry ice or liquid nitrogen. This results in the instantaneous freezing of the droplets. The trays are then loaded into a lyophilizer and the particles are then freeze-dried according to the process described above.
- each solid DNA delivery dose may be loaded with relatively high amounts of DNA.
- the formulations resulting from the above techniques may be used directly or after milling and sieving into reservoir medium coated dense core beads.
- the vaccine formulations of the present invention contain DNA that encodes an antigen or antigenic composition capable of eliciting an immune response against a human pathogen, which antigen or antigenic composition is derived from HIN-1, (such as tat, nef, gpl20 or gpl60), human herpes viruses, such as gD or derivatives thereof or Immediate Early protein such as ICP27 from HSN1 or HSN2, cytomegalovirus ((esp Human)(such as gB or derivatives thereof), Rotavirus (including live-attenuated viruses), Epstein Barr virus (such as gp350 or derivatives thereof), Varicella Zoster Virus (such as gpl, II and IE63), or from a hepatitis virus such as hepatitis B virus (for example Hepatitis B Surface antigen or a derivative thereof), hepatitis A virus, hepatitis C virus and hepatitis E virus, or from other viral pathogens, such
- Influenza virus whole live or inactivated virus, split influenza virus, grown in eggs or MDCK cells, or Vero cells or whole flu virosomes (as described by R. Gluck, Vaccine, 1992, 10, 915-920) or purified or recombinant proteins thereof, such as HA, NP, NA, or M proteins, or combinations thereof), or derived from bacterial pathogens such as Neisseria spp, including N. gonorrhea and N. meningitidis (for example capsular polysaccharides and conjugates thereof, transferrin-binding proteins, lactoferrin binding proteins, PilC, adhesins); S.
- pyogenes for example M proteins or fragments thereof, C5A protease, lipoteichoic acids), S. agalactiae, S. mutans; H. ducreyi; Moraxella spp, including M catarrhalis, also known as Branhamella catarrhalis (for example high and low molecular weight adhesins and invasins); Bordetella spp, including B. pertussis (for example pertactin, pertussis toxin or derivatives thereof, filamenteous hemagglutinin, adenylate cyclase, fimbriae), B. parapertussis and B.
- B. pertussis for example pertactin, pertussis toxin or derivatives thereof, filamenteous hemagglutinin, adenylate cyclase, fimbriae
- bronchiseptica Mycohacterium spp., including M. tuberculosis (for example ESAT6, Antigen 85A, -B or -C), M. bovis, M. leprae, M. avium, M. paratuberculosis, M. smegmatis; Legionella spp, including L. pneumophila; Escherichia spp, including enterotoxic E. coli (for example colonization factors, heat- labile toxin or derivatives thereof, heat-stable toxin or derivatives thereof), enterohemorragic E. coli, enteropathogenic E. coli (for example shiga toxin-like toxin or derivatives thereof); Vibrio spp, including V.
- M. tuberculosis for example ESAT6, Antigen 85A, -B or -C
- M. bovis M. leprae
- M. avium M. paratuberculosis
- cholera for example cholera toxin or derivatives thereof
- Shigella spp including S. sonnei, S. dysenteriae, S. flexnerii
- Yersinia spp including Y. enterocolitica (for example a Yop protein) , Y. pestis, Y. pseudotuberculosis
- Campylobacter spp including C.jejuni (for example toxins, adhesins and invasins) and C. coli
- Salmonella spp including S. typhi, S. paratyphi, S. choleraesuis, S. enteritidis
- Listeria spp. including L.
- H. pylori for example urease, catalase, vacuolating toxin
- Pseudomonas spp including P. aeruginosa
- Staphylococcus spp. including S. aureus, S. epidermidis
- Enter ococcus spp. including E.faecalis, E.faecium
- Clostridium spp. including C. tetani (for example tetanus toxin and derivative thereof), C. botulinum (for example botulinum toxin and derivative thereof), C.
- Bacillus spp. including B. anthracis (for example botulinum toxin and derivatives thereof ⁇ ; Corynebacterium spp., including C. diphtheriae (for example diphtheria toxin and derivatives thereof); Borrelia spp., including B. burgdorferi (for example OspA, OspC, DbpA, DbpB), B. garinii (for example OspA, OspC, DbpA, DbpB), B. afzelii (for example OspA, OspC, DbpA, DbpB), B.
- pallidum for example the rare outer membrane proteins
- T. denticola for example the rare outer membrane proteins
- T. hyodysenteriae or derived from parasites such as Plasmodium spp., including P. falciparum; Toxoplasma spp., including T. gondii (for example SAG2, SAG3, Tg34); Entamoeba spp., including E. histolytica; Babesia spp., including B. microti; Trypanosoma spp., including T. cruzi; Giardia spp., including G. lamblia; Leshmania spp., including . major; Pneumocystis spp., including P.
- Plasmodium spp. including P. falciparum
- Toxoplasma spp. including T. gondii (for example SAG2, SAG3, Tg34)
- Entamoeba spp. including E. his
- H. influenzae type B for example PRP and conjugates thereof
- non typeable H. influenzae for example OMP26
- high molecular weight adhesins for example P5, P6, protein D and lipoprotein D
- fimbrin and fimbrin derived peptides US 5,843,464.
- the DNA dosage form contains a DNA vaccine in combination with a non-DNA antigen such as a protein or polysaccharide antigen derived from a pathogen.
- a non-DNA antigen such as a protein or polysaccharide antigen derived from a pathogen.
- the DNA vaccine may further comprise an agent to enhance uptake of the DNA into the cell, an adjuvant or other immunostimulant to improve and or direct the immune response, and may also further comprise pharmaceutically acceptable excipient(s).
- the solid pharmaceutical reservoir medium may preferably contain a DNA condensing agent for example spermidine or PEI (polyethyleneimine).
- a DNA condensing agent for example spermidine or PEI (polyethyleneimine).
- Other excipients which may be included in the formulation include buffers, amino acids, phase change inhibitors ('crystal poisoners') which may be added to prevent phase change of the coating during processing or storage or inhibitors to prevent deleterious chemical reactions during processing or storage such Maillard reaction inhibitors like amino acids.
- a preferred additional agent to the co-entrapped within the reservoir medium with the DNA is a DNAase inhibitor.
- a DNAase inhibitor which is preferred is aurinticarboxylic acid (ATA, Glasspool-Malone, J. et al., (2000), Molecular Therapy 2: 140-146).
- Vaccines of the present invention may advantageously also include an immunologically effective adjuvant in solid solution together with the DNA.
- the adjuvant maybe associated with separate microbeads to the DNA coated microbead.
- Suitable adjuvants for vaccines of the present invention comprise those adjuvants that are capable of enhancing the antibody responses against the immunogen.
- Suitable immunostimulatory agents include, but this list is by no means exhaustive and does not preclude other agents: synthetic imidazoquinolines such as imiquimod [S-26308, R-837], (Dockrell and Kinghorn, 2001, Journal of Antimicrobial Chemotherapy, 48, 751-755; Harrison, et al.
- cytokine, chemokine and co-stimulatory molecules as either protein or peptide
- Certain preferred adjuvants for eliciting a predominantly Thl -type response include, for example, a Lipid A derivative such as monophosphoryl lipid A, or preferably 3-de-O-acylated monophosphoryl lipid A.
- MPL ® adjuvants are available from Corixa Corporation (Seattle, WA; see, for example, US Patent Nos. 4,436,727; 4,877,611; 4,866,034 and 4,912,094).
- CpG-containing ohgonucleotides in which the CpG dinucleotide is unmethylated also induce a predominantly Thl response.
- Another preferred adjuvant comprises a saponin, such as Quil A, or derivatives thereof, including QS21 and QS7 (Aquila Biopharmaceuticals Inc., Framingham, MA); Escin; Digitonin; or Gypsoph ⁇ la or Chenopodium quinoa saponins.
- the preferred immunostimulatory agent or adjuvant is immiquimod or other related molecules (such as resiquimod) as described in PCT patent application publication number WO 94/17043 (the contents of which are incorporated herein by reference).
- a polynucleotide is administered/delivered as "naked" DNA, for example as described in Uhner et al., Science 259:1745-1749, 1993 and reviewed by Cohen, Science 259:1691-1692, 1993.
- the uptake of naked DNA may be increased by coating the DNA onto small microbeads beads, such as gold, or biodegradable beads, which are efficiently transported into the cells; or by using other well known transfection facilitating agents, such as Calcium Phosphate or DEAE dextran.
- the amount of expressible DNA in each vaccine administration is selected as an amount which induces an immunoprotective response without significant adverse side effects in typical vaccinees.
- each dose will generally comprise 1-1000 ⁇ g of DNA, preferably 1-500 ⁇ g, more preferably 1-100 ⁇ g, of which 1 to 50 ⁇ g is the most preferable range.
- An optimal amount for a particular vaccine can be ascertained by standard studies involving observation of appropriate immune responses in subjects. Following an initial vaccination, subjects may receive one or several booster immunisations adequately spaced.
- ballistic delivery devices loaded with the DNA dosage forms of the present invention.
- the formulations of the present invention may be used for both prophylactic and therapeutic purposes. Accordingly, the present invention provides for a method of treating a mammal susceptible to or suffering from an infectious disease or cancer, or allergy, or autoimmune disease. In a further aspect of the present invention there is provided a vaccine as herein described for use in medicine. Vaccine preparation is generally described in New Trends and Developments in Vaccines, edited by Voller et al., University Park Press, Baltimore, Maryland, U.S.A. 1978. The present invention is exemplified by, but not limited to, the following examples.
- Example 1 Demonstration of coating of microbeads with a reservoir medium comprising plasmid DNA.
- pEGFP-Cl is a GFP expression vector, (Clontech, Palo Alto, California, USA).
- pGL3CMV is a luciferase expression vector based upon pGL3 Basic, (Promega
- pVACl.ova is a chicken ovalbumin expression plasmid, constructed by ligating PCR amplified cDNA encoding chicken ovalbumin from pUGOVA, into the expression vector pVACl .
- pNACl is a modification of the mammalian expression vector, pCI, (Promega), where the multiple cloning site, from EcoRI to Bst ZI, has been replaced by the EMCN IRES sequence flanked 5' by unique ⁇ he I, Rsr H and Xho I and 3' by unique Pac I, Asc I and Not I restriction enzyme sites, amplified from pGL3Basic, (Promega).
- Supercoiled plasmid DNA, (low endotoxin) was purified on a large scale, aproximately lOOmg yield, to high purity using a combination of alkaline SDS lysis, ultrafiltration and anion exchange column chromatography.
- Plasmids were resuspended in TE, (lOmM TrisHCl, lmM EDTA), pH 8.0 at lug / ul. And determined as >95% supercoiled upon analysis by agarose gel electrophoresis. Plasmids were formulated in a variety of solutions, for coating needles, by a standard large-scale ethanol precipitation procedure. The precipitated DNA was resuspended directly into the aqueous formulation solutions at concentrations of 0.5 to 12 ug/ul, (See Chapter 1, Molecular Cloning: A Laboratory Manual, Sambrook, J. et al, 2 nd Edition, 1989, CSH laboratory Press, Cold Spring Harbor, New York, USA).
- Spray drying is a dehydration process that utilizes heat from a hot gas stream (usually air) to evaporate dispersed droplets created by atomization of a continuous liquid feed. Resulting powder products dry within a few seconds into fine particles.
- the feasibility of spray drying for generating therapeutic protein powders has been amply demonstrated ((Broadhead, J., Rouan, S.K.E., Hau, I., and Rhodes, C.T. 1994. J. Pharm. Pharmacol. 46: 458-467.; Mumenthaler, M., Hsu, C.C., and Pearlman, R. 1994. Pharm. Res. 11 : 12-20)).
- the formulated DNA, gold particles and sugar solution will be fed into a spray dryer with a typical inlet temperature in the range of 50 to 150C typically at a flow rate between 0.1 and 10 mL/min.
- the resulting powder is dry and is collected from the collection chamber.
- Spray freeze-drying is a process in which the solution containing the DNA, gold particles and sugars is sprayed onto trays containing dry ice or liquid nitrogen. This results in the instantaneous freezing of the droplets. The trays are then loaded into a lyophilizer and the particles are then freeze-dried according to the process described above.
- the formulations resulting from the above techniques may be used directly or after milling in conventional ballistic delivery devices, and expression in target cells may be followed by observing luciferase expression.
- the samples are also stable as measured by maintenance of supercoiled structure.
- Example 2 Stability of plasmid DNA, in different sugar formulations, after coating, lyophilization and storage on needles, at 37° C.
- Plasmid DNA (pNACl.ONA) was then eluted and recovered in the standard manner and subject to agarose gel electrophoresis, (100V, 100mA for 2 hours), in the absence of intercalating agents, (Sambrook, J. et al, supra). The integrity of the eluted plasmid D ⁇ A was then monitored after staining with ethidium bromide and visualisation under UV light. The percentage of supercoiled monomeric and dimeric plasmid forms and also any linear and open circular forms from these samples were measured as image intensity using the Labworks 4.0 image analysis software on the UVP Bioimaging System.
- the data is displayed in FIG 2, as a graphical plot of the percentage of supercoiled plasmid, (%ccc), both monomeric, (%cccmon), and dimeric, (%cccdim), plasmid forms; after coating and lyophilization onto sowing needles and storage at 37°C.
- the plasmid formulations used contain varying amounts of sugars: FIG 2A: 5% sucrose, FIG 2B: 10% sucrose, FIG 2C: 17.5% sucrose, FIG 2D: 40% sucrose, FIG 2E: 40% trehalose, FIG 2F: 40% glucose.
- Example 3 Demonstration of amorphous glass formation after lyophilization of plasmid DNA, in sucrose formulations containing excipients.
- Samples oflyophilised DNA / sucrose formulations were prepared containing plasmid DNA (pVACl.OVA), (lOmg/ml), in 40% sucrose and also lyophilised samples were prepared additionally containing lOOmM TrisHCl pH8.0, lmM EDTA, lOmM methionine and 2.9% ethanol. Samples were split and subject to either 1 hour or 24 hour lyophilization cycles. The samples were then subject to analysis by differential scanning calorimetry, (DSC), to determine the solid state form. This was performed on a TA Instruments DSC2920 machine over a temperature range from 25°C to 300°C, using nitrogen as the purge gas with a flow rate of 20ml / min. The sample pan type was pinhole aluminium and the sample weight was determined on the day of analysis on a Mettler M3 balance.
- DSC differential scanning calorimetry
- Fig 3 A & B formulations also contain: lOOmM TrisHCl pH8.0, lmM EDTA, lOmM methionine and 2.9% ethanol; Fig 3 A & C represent a 24 hour lyophilization cycle; Fig 3B & D represent a 1 hour lyophilization cycle.
- the data suggest that all the samples contain amorphous sucrose with sucrose glass transition temperatures being observed at about 78°C, (Fig 3A), 85°C, (Fig3B), 74°C, (Fig3C) and 63°C, (Fig 3D), which fit well with published values in the literature.
- FIG 4 The data is shown in FIG 4 where all formulations contain plasmid DNA, (1 Omgml), in 40% sucrose.
- Fig 4A formulations also contain: lOOmM TrisHCl pH8.0, lmM EDTA, lOmM methionine and 2.9% ethanol
- Fig 4C only contains 40% sucrose
- Fig 4D shows crystals of the major solid excipient.
- 1AM, 2 AM & 3 AM represent a 24 hour lyophilization cycle
- 1ST, 2ST & 3ST represent a 1 hour lyophilization cycle.
- Example 4 Demonstration of amorphous glass formation after lyophilization of plasmid DNA, in different sugar / polyol formulations containing excipients.
- the polyol was varied. A number of similar formulations that differed only in the polyol present were generated, lyophilised and analysed by polarised light microscopy. This was performed in a similar manner to that described in example 3 except that on this occasion an Olympus BX51 polarized light microscope was used.
- FIG 5 The data is shown in FIG 5 where all formulations contain lyophilisized plasmid DNA (pVACl .OVA), (lOmg ml), and lOOmM TrisHCl pH8.0, lmM EDTA, lOmM methiomne and 2.9% ethanol.
- Fig 5 A sample 1: 40% w/v ficoll, sample 2: 20% w/v dextran, sample 3: 40% w/v sucrose, sample 4: 20% w/v maltotriose.
- Fig 5B sample 5: 20% w/v lactose, sample 6: 30% w/v maltose, sample 7: 40% w/v glucose, sample 8: 40% w/v trehalose.
- the aim of this study was to lyophilise three sugar based DNA formulations containing gold particles.
- the formulations were formed as shown below;
- Formulation 1 made up of 40% Sucrose, lOOmM TrisHCl ⁇ H8.0, lOmM L- methionine and 2.9% ethanol
- Foimulation 2 contained 10% Sucrose, 1 OOmM TrisHCl pH8.0, 1 OmM L- methionine and 2.9% ethanol • Formulation 3 was made up of 40% Trehalose, lOOmM TrisHCl ⁇ H8.0, lOmM L- methionine and 2.9% ethanol
- Supercoiled hepatitis B plasmid was formulated into each of the three formulations at a concentration of 1 mg/mL. Gold particles were added to each of the formulations at a concentration of 0.5g per 1 OmL of formulation. The formulations were snap frozen by dropwise addition of each formulation in liquid nitrogen. The resulting frozen beads were transferred to 3mL freeze-drying vials. The vials were freeze dried in a DW8 Heto Holten freeze dryer using the cycle shown below;
- freeze-dryed samples were stoppered under vacuum. The vacuum was released and the vials removed from the freeze dryer.
- An accelerated stability study (at 25°C) was set up using the freeze-dried D ⁇ A formulations. The stability study was monitored at weekly intervals using ethidium bromide stained agarose gel electrophoresis.
- a 0.6% agarose gel electrophoresis was carried out on stability samples in order determine a change in D ⁇ A conformation during the study.
- Each lyophilised sample was reconstituted in distilled water and added to its respective well of the agarose gel using the following combination of sample, loading buffer and distilled water. 2 ⁇ l of sample + 16 ⁇ l of distilled water + 2 ⁇ l of loading buffer
- FIG 6 shows the D ⁇ A profile of samples stored at 25°C for 1 week (lane 1 on left hand side): Lane 1 - 1 kilobase ladder; Lane 2 - Freeze-dried sample (Formulation 1) at 25 °C following one week storage; Lane 3 - Freeze-dried sample (Formulation 1) at 5°C after one week storage - Control; Lane 4 - Freeze-dried sample (Formulation 2) at 25°C following one week storage, Lane 5 - Freeze-dried sample (Formulation 2) at 5°C following one week storage; Lane 6 - Freeze-dried sample (Formulation 3) at 25°C following one week storage; Lane 7 - Freeze-dried sample (Formulation 3) at 5°C following one week storage; Lane 8 - Pre freeze-dried liquid Formulation 1 at 5°C; Lane 9 - Pre freeze-dried liquid Formulation 2 at 5°C Lane 10 - Pre freeze-d
- Figure 7 shows the DNA profile of samples stored at 25°C for 3 week
- Lane 1 on the left hand side Lane 1 - 1 kilobase ladder; Lane 2 - Freeze-dried sample (Formulation 1) at 25°C following three weeks storage; Lane 3 - Freeze-dried sample (Formulation 1) at 5°C after three week storage - Control; Lane 4 - Freeze- dried sample (Formulation 2) at 25°C following three weeks storage; Lane 5 - Freeze- dried sample (Formulation 2) at 5°C following three weeks storage; Lane 6 - Freeze- dried sample (Formulation 3) at 25 °C following three weeks storage; Lane 7 - Freeze- dried sample (Formulation 3) at 5°C following three weeks storage; Lane 8 - Pre freeze-dried liquid Formulation 1 at 5°C; Lane 9 - Pre freeze-dried liquid Formulation 2 at 5°C; Lane 10 - Pre freeze-dried liquid Formulation 3 at 5°C; Lane 11 - Unformulated plasmid; Lane 12 - Freeze-dried Negative control of Formulation 1 consisting of Formulation 1
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Medicinal Preparation (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Description
Claims
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/502,290 US20050085434A1 (en) | 2002-01-25 | 2003-01-23 | Dna dosage forms |
CA002473717A CA2473717A1 (en) | 2002-01-25 | 2003-01-23 | Dna dosage forms |
EP03700947A EP1467711B1 (en) | 2002-01-25 | 2003-01-23 | Dna dosage forms |
JP2003561574A JP2005519905A (en) | 2002-01-25 | 2003-01-23 | DNA preparations |
DE60312645T DE60312645T2 (en) | 2002-01-25 | 2003-01-23 | PHARMACEUTICAL FORMS FOR DNS |
US11/756,817 US20080095854A1 (en) | 2002-01-25 | 2007-06-01 | Dna dosage forms |
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB0201735A GB0201735D0 (en) | 2002-01-25 | 2002-01-25 | DNA dosage forms |
GBGB0201736.6A GB0201736D0 (en) | 2002-01-25 | 2002-01-25 | DNA dosage forms |
GB0201735.8 | 2002-01-25 | ||
GB0201736.6 | 2002-01-25 |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US11/756,817 Continuation US20080095854A1 (en) | 2002-01-25 | 2007-06-01 | Dna dosage forms |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2003061629A2 true WO2003061629A2 (en) | 2003-07-31 |
WO2003061629A3 WO2003061629A3 (en) | 2003-10-02 |
Family
ID=27614803
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/GB2003/000336 WO2003061629A2 (en) | 2002-01-25 | 2003-01-23 | Dna dosage forms |
Country Status (8)
Country | Link |
---|---|
US (2) | US20050085434A1 (en) |
EP (1) | EP1467711B1 (en) |
JP (1) | JP2005519905A (en) |
AT (1) | ATE357219T1 (en) |
CA (1) | CA2473717A1 (en) |
DE (1) | DE60312645T2 (en) |
ES (1) | ES2285087T3 (en) |
WO (1) | WO2003061629A2 (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2004026336A1 (en) * | 2002-09-23 | 2004-04-01 | Vital Biotech (Hong Kong) Limited | Improvements in or relating to vaccines |
US7884083B2 (en) * | 2002-08-12 | 2011-02-08 | Dynavax Technologies Corporation | Immunomodulatory compositions, methods of making, and methods of use thereof |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0423681D0 (en) * | 2004-10-26 | 2004-11-24 | Sec Dep For Environment Food & | Vaccine and nucleic acids |
JP5252787B2 (en) * | 2005-08-02 | 2013-07-31 | 参天製薬株式会社 | Method for inhibiting degradation of thermally unstable drugs |
WO2007015510A1 (en) * | 2005-08-02 | 2007-02-08 | Santen Pharmaceutical Co., Ltd. | Method for prevention of degradation of thermally unstable substance |
US20120222979A1 (en) | 2011-03-04 | 2012-09-06 | Elwha LLC, a limited liability company of the State of Delaware | Glassy compositions |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995019799A1 (en) * | 1994-01-21 | 1995-07-27 | Agracetus, Inc. | Gas driven gene delivery instrument |
WO1997040839A1 (en) * | 1996-04-26 | 1997-11-06 | Merck & Co., Inc. | Dna vaccine formulations |
EP1138337A2 (en) * | 1994-08-04 | 2001-10-04 | Quadrant Holdings Cambridge Limited | Solid delivery systems for controlled release of molecules incorporated therein and methods of making same |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0431135B1 (en) * | 1989-06-26 | 2003-03-26 | Powderject Vaccines, Inc. | Particle-mediated transformation of animal somatic cells |
DK28792D0 (en) * | 1992-03-04 | 1992-03-04 | Novo Nordisk As | NEW ENZYM |
EP0696200A4 (en) * | 1993-04-19 | 1998-04-15 | Medisorb Technologies Internat | Encapsulation of nucleic acids with conjugates that facilitate and target cellular uptake and gene expression |
US6290991B1 (en) * | 1994-12-02 | 2001-09-18 | Quandrant Holdings Cambridge Limited | Solid dose delivery vehicle and methods of making same |
-
2003
- 2003-01-23 JP JP2003561574A patent/JP2005519905A/en active Pending
- 2003-01-23 WO PCT/GB2003/000336 patent/WO2003061629A2/en active IP Right Grant
- 2003-01-23 EP EP03700947A patent/EP1467711B1/en not_active Expired - Lifetime
- 2003-01-23 ES ES03700947T patent/ES2285087T3/en not_active Expired - Lifetime
- 2003-01-23 CA CA002473717A patent/CA2473717A1/en not_active Abandoned
- 2003-01-23 AT AT03700947T patent/ATE357219T1/en not_active IP Right Cessation
- 2003-01-23 DE DE60312645T patent/DE60312645T2/en not_active Expired - Fee Related
- 2003-01-23 US US10/502,290 patent/US20050085434A1/en not_active Abandoned
-
2007
- 2007-06-01 US US11/756,817 patent/US20080095854A1/en not_active Abandoned
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1995019799A1 (en) * | 1994-01-21 | 1995-07-27 | Agracetus, Inc. | Gas driven gene delivery instrument |
EP1138337A2 (en) * | 1994-08-04 | 2001-10-04 | Quadrant Holdings Cambridge Limited | Solid delivery systems for controlled release of molecules incorporated therein and methods of making same |
WO1997040839A1 (en) * | 1996-04-26 | 1997-11-06 | Merck & Co., Inc. | Dna vaccine formulations |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7884083B2 (en) * | 2002-08-12 | 2011-02-08 | Dynavax Technologies Corporation | Immunomodulatory compositions, methods of making, and methods of use thereof |
WO2004026336A1 (en) * | 2002-09-23 | 2004-04-01 | Vital Biotech (Hong Kong) Limited | Improvements in or relating to vaccines |
Also Published As
Publication number | Publication date |
---|---|
EP1467711B1 (en) | 2007-03-21 |
WO2003061629A3 (en) | 2003-10-02 |
US20080095854A1 (en) | 2008-04-24 |
ATE357219T1 (en) | 2007-04-15 |
US20050085434A1 (en) | 2005-04-21 |
ES2285087T3 (en) | 2007-11-16 |
JP2005519905A (en) | 2005-07-07 |
DE60312645D1 (en) | 2007-05-03 |
CA2473717A1 (en) | 2003-07-31 |
EP1467711A2 (en) | 2004-10-20 |
DE60312645T2 (en) | 2007-11-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
EP1301238B1 (en) | Needles coated with vaccine | |
JP4917263B2 (en) | Method for lyophilizing nucleic acid / block copolymer / cationic surfactant complex | |
EP1467720B1 (en) | Dna dosage forms | |
US20050266021A1 (en) | Powder compositions | |
AU2001275371B2 (en) | Powder compositions | |
US20080095854A1 (en) | Dna dosage forms | |
MXPA03001392A (en) | Oral solid dose vaccine. | |
EP1528914B1 (en) | Antigenic compositions | |
AU2001275371A1 (en) | Powder compositions |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A2 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SC SD SE SG SK SL TJ TM TN TR TT TZ UA UG US UZ VC VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A2 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LU MC NL PT SE SI SK TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2003700947 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2473717 Country of ref document: CA |
|
WWE | Wipo information: entry into national phase |
Ref document number: 10502290 Country of ref document: US Ref document number: 2003561574 Country of ref document: JP |
|
WWP | Wipo information: published in national office |
Ref document number: 2003700947 Country of ref document: EP |
|
WWG | Wipo information: grant in national office |
Ref document number: 2003700947 Country of ref document: EP |