WO2003059293A2 - 17-hydroxy-4-aza-androstan-3-ones as androgen receptor modulators - Google Patents

17-hydroxy-4-aza-androstan-3-ones as androgen receptor modulators Download PDF

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WO2003059293A2
WO2003059293A2 PCT/US2003/000874 US0300874W WO03059293A2 WO 2003059293 A2 WO2003059293 A2 WO 2003059293A2 US 0300874 W US0300874 W US 0300874W WO 03059293 A2 WO03059293 A2 WO 03059293A2
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hydroxy
aza
methyl
androst
aryl
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PCT/US2003/000874
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French (fr)
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WO2003059293A3 (en
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William P. Dankulich
Robert S. Meissner
Helen J. Mitchell
James J. Perkins
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Merck & Co., Inc.
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Priority to AU2003219656A priority Critical patent/AU2003219656B2/en
Priority to US10/501,640 priority patent/US7605152B2/en
Priority to JP2003559458A priority patent/JP4563032B2/en
Priority to CA2472475A priority patent/CA2472475C/en
Priority to EP03715925A priority patent/EP1467739A4/en
Publication of WO2003059293A2 publication Critical patent/WO2003059293A2/en
Publication of WO2003059293A3 publication Critical patent/WO2003059293A3/en

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07J—STEROIDS
    • C07J73/00—Steroids in which the cyclopenta[a]hydrophenanthrene skeleton has been modified by substitution of one or two carbon atoms by hetero atoms
    • C07J73/001—Steroids in which the cyclopenta[a]hydrophenanthrene skeleton has been modified by substitution of one or two carbon atoms by hetero atoms by one hetero atom
    • C07J73/005—Steroids in which the cyclopenta[a]hydrophenanthrene skeleton has been modified by substitution of one or two carbon atoms by hetero atoms by one hetero atom by nitrogen as hetero atom
    • A—HUMAN NECESSITIES
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/47—Quinolines; Isoquinolines
    • A61K31/473—Quinolines; Isoquinolines ortho- or peri-condensed with carbocyclic ring systems, e.g. acridines, phenanthridines
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    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/56—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids
    • A61K31/58—Compounds containing cyclopenta[a]hydrophenanthrene ring systems; Derivatives thereof, e.g. steroids containing heterocyclic rings, e.g. danazol, stanozolol, pancuronium or digitogenin
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    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
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    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
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    • A61P5/00—Drugs for disorders of the endocrine system
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Definitions

  • the androgen receptor belongs to the superfamily of steroid thyroid hormone nuclear receptors, whose other members include the estrogen receptor (ER), the progesterone receptor (PR), the glucocorticoid receptor (GR), and the mineralocorticoid receptor (MR).
  • the AR is expressed in numerous tissues of the body and is the receptor through which the physiological as well as the pathophysiological effects of endogenous androgen ligands, such as testosterone (T) and dihydrotestosterone (DHT), are expressed.
  • the AR is composed of three main functional domains: the ligand binding domain (LBD), the DNA-binding domain, and amino-terminal domain.
  • LBD ligand binding domain
  • a compound that binds to the AR and mimics the effects of an endogenous AR ligand is referred to as an AR agonist, whereas a compound that inhibits the effects of an endogenous AR ligand is termed an AR antagonist.
  • Androgen ligand binding to the AR affords a ligand/receptor complex, which, subsequent to translocation inside the nucleus of the cell, binds to specific regulatory DNA sequences (referred to as androgen response elements or AREs) within the promoter or enhancer regions of the target gene or genes present in the cell's nucleus.
  • AREs specific regulatory DNA sequences
  • cof actors are next recruited which bind to the amino-terminal domain or the LBD of the receptor leading to gene transcription and subsequent translation to produce the protein(s) encoded by that gene or genes.
  • Androgen therapy has been used in the clinic to treat a variety of male disorders, such as reproductive disorders and primary or secondary male hypogonadism.
  • AR agonists have been clinically investigated for the treatment of musculoskeletal disorders, such as bone disease, hematopoietic disorders, neuromuscular disease, rheumatological disease, wasting disease, and for hormone replacement therapy (HRT), such as female androgen deficiency.
  • AR antagonists such as flutamide and bicalutamide, have been used to treat prostate cancer.
  • estrogen - progestin combinations that incorporated an androgenic progestin (such as norethindrone), rather than medroxyprogesterone acetate, yielded greater improvements in hip BMD.
  • CEE oral conjugated estrogen
  • methyltestosterone combinations were demonstrated to be effective in promoting accrual of bone mass in the spine and hip, while conjugated estrogen therapy alone prevented bone loss ["A two-year, double-blind comparison of estrogen-androgen and conjugated estrogens in surgically menopausal women: Effects on bone mineral density, symptoms and lipid profiles," J. Reprod. Med., 44: 1012-1020 (1999)].
  • a common scenario for androgen deficiency occurs in men with stage D prostate cancer (metastatic) who undergo androgen deprivation therapy (ADT). Endocrine orchiectomy is achieved by long acting GnRH agonists, while androgen receptor blockade is implemented with flutamide, nilutamide, bicalutamide, or RU 58841 (AR antagonists). In response to hormonal deprivation, these men suffered from hot flushes, significant bone loss, weakness, and fatigue. In a recent pilot study of men with stage D prostate cancer, osteopenia (50% vs. 38%) and osteoporosis (38% vs.
  • tissue selective AR antagonists in the prostate that lack antagonistic action in bone and muscle may be useful agents for the treatment of prostate cancer, either alone or as an adjunct to traditional ADT such as with a GnRH agonist/antagonist [See also A. Stoch, et al., J. Clin. Endocrin.
  • Tissue-selective AR antagonists may also have utility in the treatment of polycystic ovarian syndrome in postmenopausal women [see C.A. Eagleson, et al., "Polycystic ovarian syndrome: evidence that flutamide restores sensitivity of the gonadotropin-releasing hormone pulse generator to inhibition by estradiol and progesterone," J. Clin. Endocrinol. Metab., 85: 4047- 4052 (2000) and E. Diamanti-Kandarakis, "The Effect of a Pure Antiandrogen Receptor Blocker, Flutamide, on the Lipid Profile in the Polycystic Ovary Syndrome," Int. J.
  • Osteoporosis is characterized by bone loss, resulting from an imbalance between bone resorption (destruction) and bone formation, which starts in the fourth decade and continues throughout life at the rate of about 1-4% per year [Eastell, "Treatment of postmenopausal osteoporosis,” New Engl. J. Med., 338: 736 (1998)].
  • Eastell "Treatment of postmenopausal osteoporosis," New Engl. J. Med., 338: 736 (1998).
  • the increase in spinal BMD attained by bisphosphonate treatment can reach 11% after 7 years of treatment with alendronate.
  • the rate of bone turnover differs from site to site, higher in the trabecular bone of the vertebrae than in the cortex of the long bones, the bone resorption inhibitors are less effective in increasing hip BMD and preventing hip fracture. Therefore, osteoanabolic agents, which increase cortical bone formation and bone mass of long bones by stimulating periosteal bone formation, would address an unmet need in the treatment of osteoporosis especially for patients with high risk of hip fractures.
  • the osteoanabolic agents also complement the bone resorption inhibitors that target the trabecular envelope, leading to a biomechanically favorable bone structure (Schmidt, et al., "Anabolic steroid: Steroid effects on bone in women," In: J. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press, 1996).
  • Tissue-selective AR agonists with diminished deleterious effects on the cardiovascular system and limited virilizing potential may be useful as a monotherapy for the prevention and/or treatment of female osteoporosis.
  • a compound with osteoanabolic properties in bone and muscle but with reduced activity in the prostate and sex accessory tissues may be useful for the prevention and/or treatment of male osteoporosis and osteopenia in men, particularly elderly men.
  • Selective androgen receptor modulators may also be useful to treat certain hematopoietic disorders. It is known that androgens stimulate renal hypertrophy and erythropoietin (EPO) production. Prior to the introduction of recombinant human EPO, androgens were employed to treat anemia caused by chronic renal failure.
  • Non-steroidal SARMs were disclosed in J.P. Edwards, "New Nonsteroidal Androgen Receptor Modulators Based on 4-(Trifluoromethyl)-2(lH)-Pyrrolidino[3,2-g]quinolinone," Bioorg. Med. Chem. Lett., 8: 745-750 (1998) and in L. Zhi et al., "Switching Androgen Receptor Antagonists to Agonists by Modifying C-ring Substituents on Piperidino[3,4- g]quinoIinone," Bioorg. Med. Chem. Lett., 9: 1009-1012 (1999).
  • SARM compounds in this invention exhibit tissue selective AR agonism in vivo, i.e. agonism in bone (stimulation of bone formation in a rodent model of osteoporosis) and antagonism in prostate (minimal effects on prostate growth in castrated rodents and antagonism of prostate growth induced by AR agonists).
  • the compounds of the present invention identified as SARMs are useful to treat diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration.
  • Such compounds are ideal for the treatment of osteoporosis in women and men as a monotherapy or in combination with inhibitors of bone resorption, such as bisphosphonates, estrogens, SERMs, cathepsin K inhibitors, ⁇ v ⁇ 3 integrin receptor antagonists, calcitonin, and proton pump inhibitors. They can also be used with agents that stimulate bone formation, such as parathyroid hormone or analogs thereof.
  • the SARM compounds of the present invention may also be employed for treatment of prostate disease, such as prostate cancer and benign prostatic hyperplasia (BPH).
  • BPH benign prostatic hyperplasia
  • compounds of this invention exhibit minimal effects on skin (acne and facial hair growth) and may be useful for treatment of hirsutism. Additionally, compounds of this invention can stimulate muscle growth and may be useful for treatment of sarcopenia and frailty. They can be employed to reduce subcutaneous and visceral abdominal fat in the treatment of obesity. Moreover, compounds of this invention can exhibit androgen agonism in the central nervous system and may be useful to treat vasomotor symptoms (hot flush) and to increase energy and libido, particularly in postmenopausal women.
  • the compounds of the present invention may be used in the treatment of prostate cancer, either alone or as an adjunct to traditional GnRH agonist/antagonist therapy, for their ability to restore bone, or as a replacement for antiandrogen therapy because of their ability to antagonize androgen in the prostate, and minimize bone depletion in the skeletal system. Further, the compounds of the present invention may be used for their ability to restore bone in the treatment of pancreatic cancer as an adjunct to treatment with antiandrogen, or as monotherapy for their antiandrogenic properties, offering the advantage over traditional antiandrogens of being bone-sparing.
  • compounds of this invention can increase the number of blood cells, such as red blood cells and platelets, and may be useful for the treatment of hematopoietic disorders, such as aplastic anemia.
  • compounds of this invention have minimal effects on lipid metabolism.
  • tissue selective androgen receptor agonism listed above the compounds of this invention are ideal for hormone replacement therapy in hypogonadic (androgen deficient) men.
  • compositions comprising the compounds of the present invention in association with a pharmaceutically acceptable carrier. It is another object of the present invention to provide pharmaceutical compositions comprising the steroid derivatives for use as selective androgen receptor modulators.
  • the present invention provides a method for modulating a function mediated by the androgen receptor in a tissue selective manner in a patient in need of such modulation, comprising administering to the patient a therapeutically effective amount of a compound of structural formula I:
  • Rl is hydrogen, hydroxymethyl, or Ci-3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
  • R2 is hydrogen, fluorine, or Ci_4 alkyl when "a” represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or C ⁇ _4 alkyl when "a” represents a single bond;
  • R3 is hydrogen or C ⁇ _3 alkyl
  • R4 is hydrogen, Cl-4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
  • the present invention is also concerned with a method of activating the function of the androgen receptor in a patient, and, in particular, a method wherein the function of the androgen receptor is activated (agonized) in bone and/or muscle tissue and blocked in the prostate of a male patient or in the uterus of a female patient with a compound of structural formula I.
  • the compounds of formula I are useful in the prevention and/or treatment of diseases or conditions caused by androgen deficiency or which can be ameliorated by androgen replacement.
  • These diseases or conditions include osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, postmenopausal symptoms in women, atherosclerosis, hypercholesterolemia, obesity, hyperlipidemia, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis and joint repair.
  • the compounds of the present invention may be used alone or in combination with other active agents.
  • the compounds of the present invention are useful for the prevention and/or treatment of male and female osteoporosis.
  • the present invention is also concerned with novel compounds which are selective androgen receptor modulators, pharmaceutical compositions containing these novel compounds in association with a pharmaceutically acceptable carrier, and methods to treat diseases or conditions caused by androgen deficiency or which can be ameliorated by androgen replacement with the novel compounds of the present invention.
  • the present invention provides a method for modulating a function mediated by the androgen receptor in a tissue selective manner in a patient in need of such modulation, comprising administering to the patient a therapeutically effective amount of a compound of structural formula I:
  • Rl is hydrogen, hydroxymethyl, or C ⁇ _3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
  • R2 is hydrogen, fluorine, or Ci-4 alkyl when "a” represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci-4 alkyl when "a” represents a single bond;
  • R3 is hydrogen or Ci_3 alkyl;
  • R4 is hydrogen, Ci_4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
  • aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, C1 _8 alkyl, C3-.8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Ci_6alkyl, amino C ⁇ -6alkyl, C ⁇ _6 alkylamino C ⁇ -6alkyl, (Ci-6 alkyl)2amino C ⁇ -6alkyl, aryl C ⁇ -6 alkylamino C ⁇ -6 a lkyl, (aryl C ⁇ -6 alkyl)2amino C ⁇ -6alkyl, Ci_6 alkylthio, aryl C ⁇ -6 a lkylthio, C ⁇ -6 alkylsulfinyl, aryl C ⁇ -6alkylsulfinyl, Ci_6 alkylsulfonyl, aryl C ⁇ -6alkyl
  • the compounds of structural formula I have the ⁇ -stereochemical configuration for the hydroxy group at the 17-position of the steroid nucleus as indicated in structural formula la below:
  • Rl is hydrogen or methyl and R3 and R4 are both hydrogen.
  • "a" represents a double bond.
  • one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen, (b) fluorine, (c) Ci-3 alkyl, wherein alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, C ⁇ _4 alkoxy, C ⁇ _3 alkoxy-C ⁇ _3 alkoxy, and C ⁇ _3 alkyloxycarbonyl; and
  • 6-membered ring optionally containing a heteroatom selected from O, S, NH,
  • NCi-4 alkyl and NCi-4 alkyloxycarbonyl.
  • one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined above.
  • Rl is hydrogen or methyl and R3 and R4 are both hydrogen.
  • "a" represents a double bond.
  • R5 is hydrogen and R6 is arylmethyl as depicted in the structural formula below:
  • Rl is hydrogen or methyl and R and R are both hydrogen.
  • "a" represents a double bond.
  • the stereochemistry at the 16-position is as depicted in the structural formula below:
  • the present invention also provides novel compounds of structural formula IT or a pharmaceutically salt thereof which are useful as selective androgen receptor modulators:
  • Rl is hydrogen, hydroxymethyl, or Ci_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
  • R2 is hydrogen, fluorine, or C1.4 alkyl when "a” represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci_4 alkyl when "a” represents a single bond; R4 is hydrogen, C ⁇ _4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen,
  • aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Ci_8 alkyl, C3_8 cycloalkyl, C3-.8 cycloheteroalkyl, aryl C ⁇ _6alkyl, amino C ⁇ -6alkyl, Ci-6 alkylamino C ⁇ -6alkyl, (C ⁇ _6 alkyl)2amino C ⁇ -6alkyl, aryl C ⁇ -6 alkylamino C ⁇ -6alkyl, (aryl C ⁇ -6 alkyl)2amino C ⁇ -6alkyl, C ⁇ _6 alkylthio, aryl C ⁇ -6 a lkylthio, Ci-6 alkylsulfinyl, aryl C ⁇ -6alkylsulfinyl, Ci-6 alkylsulfonyl, aryl C ⁇ -6alkylsulfony
  • Rl is hydrogen or methyl and R is hydrogen.
  • one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
  • alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, C ⁇ _4 alkoxy, C ⁇ _3 alkoxy-C ⁇ _3 alkoxy, and C1-.3 alkyloxycarbonyl; and
  • 6-membered ring optionally containing a heteroatom selected from O, S, NH,
  • NC ⁇ _4 alkyl and NCi-4 alkyloxycarbonyl.
  • one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined above.
  • R5 is hydrogen and R6 is arylmethyl as depicted in the structural formula below:
  • Rl is hydrogen or methyl and R4 is hydrogen.
  • "a" represents a double bond.
  • Rl is hydrogen or methyl and R4 is hydrogen.
  • "a" represents a double bond.
  • the stereochemistry at the 16-position is as depicted in the structural formula below:
  • novel compounds of the present invention are the following:
  • alkyl shall mean straight or branched chain alkanes of one to ten total carbon atoms, or any number within this range (i.e., methyl, ethyl, 1- propyl, 2-propyl, n-butyl, s-butyl, t-butyl, etc.).
  • Co alkyl (as in “C ⁇ -8 alkylaryl”) shall refer to the absence of an alkyl group.
  • alkenyl shall mean straight or branched chain alkenes of two to ten total carbon atoms, or any number within this range.
  • alkynyl shall mean straight or branched chain alkynes of two to ten total carbon atoms, or any number within this range.
  • alkylidene shall mean a straight or branched chain alkylidene group of one to ten total carbon atoms, or any number within this range.
  • cycloalkyl shall mean cyclic rings of alkanes of three to eight total carbon atoms, or any number within this range (i.e., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or cyclooctyl).
  • cycloheteroalkyl shall mean a 3- to 8- membered fully saturated heterocyclic ring containing one or two heteroatoms chosen from N, O, or S.
  • cycloheteroalkyl groups include, but are not limited to, piperidinyl, pyrrolidinyl, azetidinyl, morpholinyl, and piperazinyl.
  • cycloheteroalkyl is selected from piperidinyl, pyrrolidinyl, and morpholinyl.
  • alkoxy refers to straight or branched chain alkoxides of the number of carbon atoms specified (e.g., Ci-5 alkoxy), or any number within this range (i.e., methoxy, ethoxy, etc.).
  • aryl refers to a monocyclic or bicyclic system comprising at least one aromatic ring, wherein the monocylic or bicyclic system contains 0, 1, 2, 3, or 4 heteroatoms chosen from N, O, or S, and wherein the monocylic or bicylic system is either unsubstituted or substituted with one or more groups independently selected from halogen, aryl, Ci-8 alkyl, C3-.8 cycloalkyl, C3..8 cycloheteroalkyl, aryl C ⁇ _6alkyl, amino CQ-6 a lkyl, C ⁇ _6 alkylamino C ⁇ -6 a lkyl, (C ⁇ _6 alkyl)2amino C ⁇ -6 a lkyl, aryl C ⁇ -6 alkylamino C ⁇ -6 a lkyl, (aryl C ⁇ -6 alkyl)2amino Co-6 a lkyl, Ci-6 alkylthio, ary
  • aryl C ⁇ -6 alkoxycarbonyl C ⁇ -6alkyl hydroxycarbonyl C ⁇ _6 alkyloxy, hydroxy C ⁇ -6 a lkyl, cyano, nitro, perfluoroC ⁇ _4alkyl, perfluoroCi-4alkoxy, oxo, C ⁇ _6 alkylcarbonyloxy, aryl C ⁇ -6 a ⁇ kylcarbonyloxy, C ⁇ -6 alkylcarbonylamino, aryl C ⁇ -6 alkylcarbonylamino, Ci_6 alkylsulfonylamino, aryl C ⁇ -6 a lkylsulfonylamino, C ⁇ _6 alkoxycarbonylamino, aryl C ⁇ -6 alkoxycarbonylamino, Ci-6alkylaminocarbonylamino, aryl C ⁇ -6 a lkylaminocarbonylamino, (C ⁇ _6alkyl)2 aminocarbonylamino, (aryl C ⁇
  • the aryl group is unsubstituted, mono-, di-, or tri- substituted with one to three of the above- named substituents; more preferably, the aryl group is unsubstituted, mono- or di- substituted with one to two of the above-named substituents.
  • alkyl or aryl or either of their prefix roots appears in a name of a substituent (e.g., aryl C ⁇ -8 alkyl), it shall be interpreted as including those limitations given above for "alkyl” and "aryl.”
  • Designated numbers of carbon atoms e.g., C ⁇ -8 shall refer independently to the number of carbon atoms in an alkyl or cyclic alkyl moiety or to the alkyl portion of a larger substituent in which alkyl appears as its prefix root.
  • arylalkyl and “alkylaryl” include an alkyl portion where alkyl is as defined above and include an aryl portion where aryl is as defined above.
  • arylalkyl include, but are not limited to, benzyl, fluorobenzyl, chlorobenzyl, phenylethyl, phenylpropyl, fluorophenylethyl, chlorophenylethyl, thienylmethyl, thienylethyl, and thienylpropyl.
  • alkylaryl include, but are not limited to, toluene, ethylbenzene, propylbenzene, methylpyridine, ethylpyridine, propylpyridine and butylpyridine.
  • halogen shall include iodine, bromine, chlorine, and fluorine.
  • oxy means an oxygen (O) atom.
  • thio means a sulfur (S) atom.
  • substituted shall be deemed to include multiple degrees of substitution by a named substitutent.
  • the substituted compound can be independently substituted by one or more of the disclosed or claimed substituent moieties, singly or plurally.
  • independently substituted it is meant that the (two or more) substituents can be the same or different.
  • Ci-5 alkylcarbonylamino Cl_6 alkyl substituent is equivalent to
  • Compounds of the present invention have been found to be tissue- selective modulators of the androgen receptor (SARMs).
  • compounds of the present invention may be useful to activate the function of the androgen receptor in a patient, and in particular to activate the function of the androgen receptor in bone and/or muscle tissue and block or inhibit (“antagonize") the function of the androgen receptor in the prostate of a male patient or in the uterus of a female patient.
  • a further aspect of the present invention is concerned with compounds of structural formula I that block the function of the androgen receptor in the prostate of a male patient or in the uterus of a female patient induced by AR agonists, but not in hair-growing skin or vocal cords, and activate the function of the androgen receptor in bone and or muscle tissue, but not in organs which control blood lipid levels (e.g. liver).
  • the compounds of the present invention may be used to treat and/or prevent conditions which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, inflammatory arthritis and joint repair, HTV-wasting, cancer cachexia, muscular dystrophies, premature ovarian failure, and autoimmune disaease, alone or in combination with other active agents.
  • Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a patient in need of such treatment.
  • the compounds of the present invention may be used to treat and/or prevent conditions in a male subject which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to, osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, and muscular dystrophies, alone or in combination with other active agents.
  • Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a male patient in need of such treatment.
  • the compounds of the present invention may be used to treat and/or prevent conditions in a female subject which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to, osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, muscular dystrophies, premature ovarian failure, and autoimmune disease, alone or in combination with other active agents.
  • Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a female patient in need of such treatment.
  • the compounds of structural formula I may also be employed as adjuncts to traditional androgen depletion therapy in the treatment of prostate cancer to restore bone, minimize bone loss, and maintain bone mineral density. In this manner, they may be employed together with traditional androgen deprivation therapy, including GnRH agonists/antagonists, such as those disclosed in P. Limonta, et al., "LHRH analogues as anticancer agents: pituitary and extrapituitary sites of action," Exp. Opin. Invest. Drugs, 10: 709-720 (2001); H.J. Strieker, "Luteinizing hormone-releasing hormone antagonists," Urology, 58 (Suppl. 2A): 24-27 (2001); R.P.
  • GnRH agonists/antagonists such as those disclosed in P. Limonta, et al., "LHRH analogues as anticancer agents: pituitary and extrapituitary sites of action," Exp. Opin. Invest. Drugs
  • the compounds of the present invention may also be employed in the treatment of pancreatic cancer, either for their androgen antagonist properties or as an adjunct to an antiandrogen, such as flutamide, 2-hydroxyflutamide (the active metabolite of flutamide), nilutamide, and bicalutamide (CasodexTM).
  • an antiandrogen such as flutamide, 2-hydroxyflutamide (the active metabolite of flutamide), nilutamide, and bicalutamide (CasodexTM).
  • Compounds of structural formula I have minimal negative effects on lipid metabolism. Therefore, considering their tissue selective androgen agonistic properties, the compounds of this invention have advantages over existing approaches for hormone replacement therapy in hypogonadic (androgen deficient) men.
  • compounds of the present invention can increase the number of blood cells, such as red blood cells and platelets, and can be used for treatment of hematopoietic disorders, such as aplastic anemia.
  • Representative compounds of the present invention typically display submicromolar binding affinity for the androgen receptor.
  • Compounds of this invention are therefore useful in treating mammals suffering from disorders related to androgen receptor function.
  • Pharmacologically effective amounts of the compound, including the pharmaceutically effective salts thereof are administered to the mammal, to treat disorders related to androgen receptor function, or which can be improved by the addition of additional androgen, such as osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis and joint repair.
  • Racemic mixtures can be separated into their individual enantiomers by any of a number of conventional methods. These include chiral chromatography, derivatization with a chiral auxiliary followed by separation by chromatography or crystallization, and fractional crystallization of diastereomeric salts.
  • a compound of the present invention which functions as an "agonist" of the androgen receptor can bind to the androgen receptor and initiate a physiological or a pharmacological response characteristic of that receptor.
  • tissue-selective androgen receptor modulator refers to an androgen receptor ligand that mimics the action of a natural ligand in some tissues but not in others.
  • a "partial agonist” is an agonist which is unable to induce maximal activation of the receptor population, regardless of the amount of compound applied.
  • a “full agonist” induces full activation of the androgen receptor population at a given concentration.
  • a compound of the present invention which functions as an "antagonist" of the androgen receptor can bind to the androgen receptor and block or inhibit the androgen-associated responses normally induced by a natural androgen receptor ligand.
  • salts refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids.
  • Salts derived from inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic salts, manganous, potassium, sodium, zinc, and the like. Particularly preferred are the ammonium, calcium, lithium, magnesium, potassium, and sodium salts.
  • Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as arginine, betaine, caffeine, choline, N,N'-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethyl-morpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.
  • basic ion exchange resins such as
  • salts may be prepared from pharmaceutically acceptable non-toxic acids, including inorganic and organic acids.
  • acids include acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, formic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, malonic, mucic, nitric, pamoic, pantothenic, phosphoric, propionic, succinic, sulfuric, tartaric, p- toluenesulfonic acid, trifluoroacetic acid, and the like.
  • Particularly preferred are citric, fumaric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric, and tartaric acids.
  • composition means the amount the compound of structural formula I that will elicit the biological or medical response of a tissue, system, animal or human that is being sought by the researcher, veterinarian, medical doctor or other clinician.
  • composition as used herein is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts.
  • pharmaceutically acceptable it is meant that the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not be deleterious to the recipient thereof.
  • administering a compound should be understood to mean providing a compound of the invention or a prodrug of a compound of the invention to the individual in need of treatment.
  • modulating a function mediated by the androgen receptor in a tissue selective manner is meant modulating a function mediated by the androgen receptor selectively (or discriminately) in anabolic (bone and/or muscular) tissue (bone and muscular) in the absence of such modulation at androgenic (reproductive) tissue, such as the prostate, testis, seminal vesicles, ovary, uterus, and other sex accessory tissues.
  • the function of the androgen receptor in anabolic tissue is activated whereas the function of the androgen receptor in androgenic tissue is blocked or suppressed.
  • the administration of the compound of structural formula I in order to practice the present methods of therapy is carried out by administering an effective amount of the compound of structural formula I to the patient in need of such treatment or prophylaxis.
  • the need for a prophylactic administration according to the methods of the present invention is determined via the use of well-known risk factors.
  • the effective amount of an individual compound is determined, in the final analysis, by the physician in charge of the case, but depends on factors such as the exact disease to be treated, the severity of the disease and other diseases or conditions from which the patient suffers, the chosen route of administration, other drugs and treatments which the patient may concomitantly require, and other factors in the physician's judgment.
  • the daily dosage of the compound of structural formula I may be varied over a wide range from 0.01 to 1000 mg per adult human per day. Most preferably, dosages range from 0.1 to 200 mg/day.
  • the compositions are preferably provided in the form of tablets containing 0.01 to 1000 mg, particularly 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 3.0, 5.0, 6.0, 10.0, 15.0, 25.0, 50.0, 75, 100, 125, 150, 175, 180, 200, 225, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.
  • the dose may be administered in a single daily dose or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, based on the properties of the individual compound selected for administration, the dose may be administered less frequently, e.g., weekly, twice weekly, monthly, etc. The unit dosage will, of course, be correspondingly larger for the less frequent administration.
  • Exemplifying the invention is a pharmaceutical composition comprising any of the compounds described above and a pharmaceutically acceptable carrier. Also exemplifying the invention is a pharmaceutical composition made by combining any of the compounds described above and a pharmaceutically acceptable carrier. An illustration of the invention is a process for making a pharmaceutical composition comprising combining any of the compounds described above and a pharmaceutically acceptable carrier.
  • Formulations of the tissue-selective androgen receptor modulator employed in the present method for medical use comprise the compound of structural formula I together with an acceptable carrier thereof and optionally other therapeutically active ingredients.
  • the carrier must be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not being deleterious to the recipient subject of the formulation.
  • the present invention therefore, further provides a pharmaceutical formulation comprising the compound of structural formula I together with a pharmaceutically acceptable carrier thereof.
  • the formulations include those suitable for oral, rectal, intravaginal, topical or parenteral (including subcutaneous, intramuscular and intravenous administration).
  • Preferred formulations are those suitable for oral administration.
  • the formulations may be presented in a unit dosage form and may be prepared by any of the methods known in the art of pharmacy. All methods include the step of bringing the active compound in association with a carrier which constitutes one or more ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active compound in association with a liquid carrier, a waxy solid carrier or a finely divided solid carrier, and then, if needed, shaping the product into the desired dosage form.
  • Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, tablets or lozenges, each containing a predetermined amount of the active compound; as a powder or granules; or a suspension or solution in an aqueous liquid or non-aqueous liquid, e.g., a syrup, an elixir, or an emulsion.
  • a tablet may be made by compression or molding, optionally with one or more accessory ingredients.
  • Compressed tablets may be prepared by compressing in a suitable machine the active compound in a free flowing form, e.g., a powder or granules, optionally mixed with accessory ingredients, e.g., binders, lubricants, inert diluents, disintegrating agents or coloring agents.
  • Molded tablets may be made by molding in a suitable machine a mixture of the active compound, preferably in powdered form, with a suitable carrier.
  • Suitable binders include, without limitation, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethyl- cellulose, polyethylene glycol, waxes and the like.
  • Lubricants used in these dosage forms include, without limitation, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like.
  • Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.
  • Oral liquid forms such as syrups or suspensions in suitably flavored suspending or dispersing agents such as the synthetic and natural gums, for example, tragacanth, acacia, methyl cellulose and the like, may be made by adding the active compound to the solution or suspension. Additional dispersing agents which may be employed include glycerin and the like.
  • Formulations for vaginal or rectal administration may be presented as a suppository with a conventional carrier, i.e., a base that is nontoxic and nonirritating to mucous membranes, compatible with the compound of structural formula I, and is stable in storage and does not bind or interfere with the release of the compound of structural formula I.
  • Suitable bases include: cocoa butter (theobroma oil), polyethylene glycols (such as carbowax and polyglycols), glycol-surfactant combinations, polyoxyl 40 stearate, polyoxyethylene sorbitan fatty acid esters (such as Tween, Myrj, and Arlacel), glycerinated gelatin, and hydrogenated vegetable oils.
  • cocoa butter theobroma oil
  • polyethylene glycols such as carbowax and polyglycols
  • glycol-surfactant combinations such as polyoxyl 40 stearate
  • polyoxyethylene sorbitan fatty acid esters such as Tween, Myrj, and Arlacel
  • glycerinated gelatin When glycerinated gelatin suppositories are used, a preservative such as methylparaben or propylparaben may be employed.
  • Topical preparations containing the active drug component can be admixed with a variety of carrier materials well known in the art, such as, e.g., alcohols, aloe vera gel, allantoin, glycerine, vitamin A and E oils, mineral oil, PPG2 myristyl propionate, and the like, to form, e.g., alcoholic solutions, topical cleansers, cleansing creams, skin gels, skin lotions, and shampoos in cream or gel formulations.
  • the compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles.
  • Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phosphatidylcholines.
  • Compounds of the present invention may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled.
  • the compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers.
  • Such polymers can include polyvinyl- pyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxy-ethylaspartamidephenol, or polyethylene-oxide polylysine substituted with palmitoyl residues.
  • the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross- linked or amphipathic block copolymers of hydrogels.
  • a drug for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross- linked or amphipathic block copolymers of hydrogels.
  • Formulations suitable for parenteral administration include formulations that comprise a sterile aqueous preparation of the active compound which is preferably isotonic with the blood of the recipient. Such formulations suitably comprise a solution or suspension of a compound that is isotonic with the blood of the recipient subject. Such formulations may contain distilled water, 5% dextrose in distilled water or saline and the active compound. Often it is useful to employ a pharmaceutically and pharmacologically acceptable acid addition salt of the active compound that has appropriate solubility for the solvents employed. Useful formulations also comprise concentrated solutions or solids comprising the active compound which on dilution with an appropriate solvent give a solution suitable for parenteral administration.
  • the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and cross-linked or amphipathic block copolymers of hydrogels.
  • biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and cross-linked or amphipathic block copolymers of hydrogels.
  • the pharmaceutical composition and method of the present invention may further comprise other therapeutically active compounds usually applied in the treatment and prevention of the above mentioned conditions, including osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis, and joint repair.
  • other therapeutically active compounds usually applied in the treatment and prevention of the above mentioned conditions, including osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis, and joint repair.
  • the compounds of the present invention may be administered in combination with a bone-strengthening agent selected from antiresorptive agents, osteoanabolic agents, and other agents beneficial for the skeleton through mechanisms which are not precisely defined, such as calcium supplements, flavonoids, and vitamin D analogs.
  • a bone-strengthening agent selected from antiresorptive agents, osteoanabolic agents, and other agents beneficial for the skeleton through mechanisms which are not precisely defined, such as calcium supplements, flavonoids, and vitamin D analogs.
  • the conditions of periodontal disease, bone fracture, and bone damage following bone reconstructive surgery may also benefit from these combined treatments.
  • the compounds of the instant invention may be effectively administered in combination with effective amounts of other agents such as estrogens, bisphosphonates, SERMs, cathepsin K inhibitors, v ⁇ 3 integrin receptor antagonists, vacuolar ATPase inhibitors, the polypeptide osteoprotegerin, antagonists of VEGF, thiazolidinediones, calcitonin, protein kinase inhibitors, parathyroid hormone (PTH) and analogs, calcium receptor antagonists, growth hormone secretagogues, growth hormone releasing hormone, insulin-like growth factor, bone morphogenetic protein (BMP), inhibitors of BMP antagonism, prostaglandin derivatives, fibroblast growth factors, vitamin D and derivatives thereof, vitamin K and derivatives thereof, soy isoflavones, calcium salts, and fluoride salts.
  • other agents such as estrogens, bisphosphonates, SERMs, cathepsin K inhibitors, v ⁇ 3 integrin receptor antagonists, vacuolar ATPase
  • a compound of the instant invention may be effectively administered in combination with an effective amount of a bone-strengthening agent selected from the group consisting of estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative; a bisphosphonate; an antiestrogen or a selective estrogen receptor modulator; an ⁇ v ⁇ 3 integrin receptor antagonist; a cathepsin K inhibitor; an osteoclast vacuolar ATPase inhibitor; calcitonin; and osteoprotegerin.
  • a bone-strengthening agent selected from the group consisting of estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative; a bisphosphonate; an antiestrogen or a selective estrogen receptor modulator; an ⁇ v ⁇ 3 integrin receptor antagonist; a cathepsin K inhibitor; an osteoclast vacuolar ATPase inhibitor; calcitonin; and osteoprotegerin.
  • the activity of the compounds of the present invention are distinct from that of the anti-resorptive agents: estrogens, bisphosphonates, SERMs, calcitonin, cathepsin K inhibitors, vacuolar ATPase inhibitors, agents interfering with the RANK/RANKL/Osteoprotegerin pathway, p38 inhibitors or any other inhibitors of osteoclast generation or osteoclast activation.
  • the compounds of structural formula I stimulate bone formation, acting preferentially on cortical bone, which is responsible for a significant part of bone strength.
  • the thickening of cortical bone substantially contributes to a reduction in fracture risk, especially fractures of the hip.
  • tissue-selective androgen receptor modulators of structural formula I with anti-resorptive agents such as estrogen, bisphosphonates, antiestrogens, SERMs, calcitonin, ⁇ v ⁇ 3 integrin receptor antagonists, HMG-CoA reductase inhibitors, vacuolar ATPase inhibitors, and cathepsin K inhibitors is particularly useful because of the complementarity of the bone anabolic and antiresorptive actions.
  • anti-resorptive agents such as estrogen, bisphosphonates, antiestrogens, SERMs, calcitonin, ⁇ v ⁇ 3 integrin receptor antagonists, HMG-CoA reductase inhibitors, vacuolar ATPase inhibitors, and cathepsin K inhibitors is particularly useful because of the complementarity of the bone anabolic and antiresorptive actions.
  • Bone antiresportive agents are those agents which are known in the art to inhibit the resorption of bone and include, for example, estrogen and estrogen derivatives which include steroidal compounds having estrogenic activity such as, for example, 17 ⁇ -estradiol, estrone, conjugated estrogen (PREMARTN®), equine estrogen, 17 ⁇ -ethynyl estradiol, and the like.
  • the estrogen or estrogen derivative may be employed alone or in combination with a progestin or progestin derivative.
  • progestin derivatives are norethindrone and medroxy- progesterone acetate.
  • Bisphosphonates are also bone anti-resorptive agents.
  • Bisphosphonate compounds which may also be employed in combination with a compound of structural formula I of the present invention include: (a) alendronic acid: (4-amino-l-hydroxybutylidene)-bis-phosphonic acid;
  • alendronate also known as alendronate sodium or monosodium trihydrate
  • (4-amino-l-hydroxybutylidene)-bis-phosphonate monosodium trihydrate alendronic acid and alendronate are described in U.S. Patents 4,922,007, to Kieczykowski et al., issued May 1, 1990, and 5,019,651, to Kieczykowski, issued May 28, 1991, both of which are incorporated by reference herein in their entirety);
  • alendronate More preferred is alendronate, pharmaceutically acceptable salts thereof, and mixtures thereof. Most preferred is alendronate monosodium trihydrate.
  • antiestrogenic compounds such as raloxifene (see, e.g., U.S. Pat. No. 5,393,763), clomiphene, zuclomiphene, enclomiphene, nafoxidene, CI- 680, CI-628, CN-55,945-27, Mer-25, U-11.555A, U-100A, and salts thereof, and the like (see, e.g., U.S. Patent Nos. 4,729,999 and 4,894,373) may be employed in combination with a compound of structural formula I in the methods and compositions of the present invention.
  • SERMs selective estrogen receptor modulators
  • agents known in the art to prevent bone loss by inhibiting bone resorption via pathways believed to be similar to those of estrogens.
  • SERMs selective estrogen receptor modulators
  • These agents may be used in combination with the compounds of the present invention to beneficially treat bone disorders including osteoporosis.
  • Such agents include, for example, tamoxifen, raloxifene, lasofoxifene, toremifene, azorxifene, EM-800, EM-652, TSE 424, clomiphene, droloxifene, idoxifene, and levormeloxifene [Goldstein, et al., "A pharmacological review of selective oestrogen receptor modulators," Human Reproduction Update, 6: 212-224 (2000), and Lufkin, et al., "The role of selective estrogen receptor modulators in the prevention and treatment of osteoporosis,” Rheumatic Disease Clinics of North America, 27: 163-185 (2001)].
  • ⁇ v ⁇ 3 Integrin receptor antagonists suppress bone resorption and may be employed in combination with the tissue selective androgen receptor modulators of structural formula I for the treatment of bone disorders including osteoporosis.
  • Peptidyl as well as peptidomimetic antagonists of the ⁇ v ⁇ 3 integrin receptor have been described both in the scientific and patent literature. For example, reference is made to W.J. Hoekstra and B.L. Poulter, Curr. Med. Chem.
  • Still other benzazepine, benzodiazepine and benzocycloheptene ⁇ v ⁇ 3 integrin receptor antagonists are described in the following patent publications: WO 96/00574, WO 96/00730, WO 96/06087, WO 96/26190, WO 97/24119, WO 97/24122, WO 97/24124, WO 98/14192, WO 98/15278, WO 99/05107, WO 99/06049, WO 99/15170, WO 99/15178, WO 99/15506, and U.S. Patent No. 6,159,964, and WO 97/34865.
  • ⁇ v ⁇ 3 integrin receptor antagonists having dibenzocycloheptene, dibenzocycloheptane and dibenzoxazepine scaffolds have been described in WO 97/01540, WO 98/30542, WO 99/11626, WO 99/15508, WO 00/33838, U.S. Patent Nos. 6,008,213, and 6,069,158.
  • Other osteoclast integrin receptor antagonists incorporating backbone conformational ring constraints have been described in the patent literature.
  • Published patent applications or issued patents disclosing antagonists having a monocyclic ring constraint include WO 99/26945, WO 99/30709, WO 99/30713, WO 99/31099, WO 99/59992, WO 00/00486, WO 00/09503, EP 0796,855, EP 0 928,790, EP 0 928,793, U.S. Patent Nos. 5,710,159; 5,723,480; 5,981,546; 6,017,926; and 6,066,648.
  • Published patent applications or issued patents disclosing antagonists having a bicyclic ring constraint include WO 98/23608, WO 98/35949, WO 99/33798, EP 0 853,084, U.S.
  • Cathepsin K formerly known as cathepsin O2
  • cathepsin O2 is a cysteine protease and is described in PCT International Application Publication No. WO 96/13523, published May 9, 1996; U.S. Patent No. 5,501,969, issued March 3, 1996; and U.S. Patent No. 5,736,357, issued April 7, 1998, all of which are incorporated by reference herein in their entirety.
  • Cysteine proteases specifically cathepsins, are linked to a number of disease conditions, such as tumor metastasis, inflammation, arthritis, and bone remodeling. At acidic pH's, cathepsins can degrade type-I collagen.
  • Cathepsin protease inhibitors can inhibit osteoclastic bone resorption by inhibiting the degradation of collagen fibers and are thus useful in the treatment of bone resorption diseases, such as osteoporosis.
  • HMG-CoA reductase inhibitors Members of the class of HMG-CoA reductase inhibitors, known as the "statins," have been found to trigger the growth of new bone, replacing bone mass lost as a result of osteoporosis (see The Wall Street Tournal, Friday, December 3, 1999, page B 1). Therefore, the statins hold promise for the treatment of bone resorption.
  • HMG-CoA reductase inhibitors include statins in their lactonized or dihydroxy open acid forms and pharmaceutically acceptable salts and esters thereof, including but not limited to lovastatin (see US Patent No. 4,342,767); simvastatin (see US Patent No.
  • Osteoclast vacuolar ATPase inhibitors also called proton pump inhibitors, may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • the proton ATPase which is found on the apical membrane of the osteoclast has been reported to play a significant role in the bone resorption process. Therefore, this proton pump represents an attractive target for the design of inhibitors of bone resorption which are potentially useful for the treatment and prevention of osteoporosis and related metabolic diseases [see C. Farina et al., "Selective inhibitors of the osteoclast vacuolar proton ATPase as novel bone antiresorptive agents," DDT, 4: 163-172 (1999)].
  • VEGF angiogenic factor
  • Activators of the peroxisome proliferator-activated receptor- ⁇ such as the thiazolidinediones (TZD's), inhibit osteoclast-like cell formation and bone resorption in vitro.
  • PPAR ⁇ activators include the glitazones, such as troglitazone, pioglitazone, rosiglitazone, and BRL 49653.
  • Calcitonin may also be employed together with the tissue selective androgen receptor modulator of structural formula I. Calcitonin is preferentially employed as salmon nasal spray (Azra et al., Calcitonin. 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Silverman, "Calcitonin," Rheumatic Disease Clinics of North America, 27: 187-196, 2001)
  • Protein kinase inhibitors may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Kinase inhibitors include those disclosed in WO 01/17562 and are in one embodiment selected from inhibitors of P-38.
  • Specific embodiments of P-38 inhibitors useful in the present invention include SB 203580 [Badger et al., "Pharmacological profile of SB 203580, a selective inhibitor of cytokine suppressive binding protein/p38 kinase, in animal models of arthritis, bone resorption, endotoxin shock, and immune function," T. Pharmacol. Exp. Ther.. 279: 1453-1461 (1996)].
  • Osteoanabolic agents are those agents that are known in the art to build bone by increasing the production of the bone protein matrix.
  • Such osteoanabolic agents include, for example, the various forms of parathyroid hormone (PTH) such as naturally occurring PTH (1-84), PTH (1-34), analogs thereof, native or with substitutions and particularly parathyroid hormone subcutaneous injection.
  • PTH parathyroid hormone
  • PTH has been found to increase the activity of osteoblasts, the cells that form bone, thereby promoting the synthesis of new bone.
  • Modes Modern Drug Discovery, Vol. 3, No. 8, 2000.
  • women in combined PTH-estrogen therapy exhibited a 12.8% increase in spinal bone mass and a 4.4% increase in total hip mass.
  • PTH and fragments thereof may prove to be efficacious in the treatment of osteoporosis alone or in combination with other agents, such as the tissue selective androgen receptor modulators of the present invention.
  • An injectable recombinant form of human PTH, Forteo has received regulatory approval in the U.S. for the treatment of osteoporosis.
  • Also useful in combination with the SARMs of the present invention are calcium receptor antagonists which induce the secretion of PTH as described by Gowen et al., in "Antagonizing the parathyroid calcium receptor stimulates parathyroid hormone secretion and bone formation in osteopenic rats," T. Clin. Invest. 105: 1595-604 (2000).
  • Growth hormone secretagogues, growth hormone, growth hormone releasing hormone and the like are also osteoanabolic agents which may be employed with the compounds according to structural formula I for the treatment of osteoporosis.
  • Representative growth hormone secretagogues are disclosed in U.S. Patent No. 3,239,345; U.S. Patent No. 4,036,979; U.S. Patent No. 4,411,890; U.S. Patent No. 5,206,235; U.S. Patent No. 5,283,241; U.S. Patent No. 5,284,841; U.S. Patent No. 5,310,737; U.S. Patent No. 5,317,017; U.S. Patent No. 5,374,721; U.S. Patent No. 5,430,144; U.S.
  • Insulin-like growth factor may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Insulin-like growth factors may be selected from Insulin-like Growth Factor I, alone or in combination with IGF binding protein 3 and IGF IT [See Tohannson and Rosen, "The IGFs as potential therapy for metabolic bone diseases," 1996, In: Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Ghiron et al., "Effects of recombinant insulin-like growth factor-I and growth hormone on bone turnover in elderly women," T. Bone Miner. Res. 10: 1844-1852 (1995)].
  • Bone morphogenetic protein may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Bone morphogenetic protein includes BMP 2, 3, 5, 6, 7, as well as related molecules TGF beta and GDF 5 [Rosen et al., "Bone morphogenetic proteins," 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Wang EA, "Bone morphogenetic proteins (BMPs): therapeutic potential in healing bony defects," Trends Biotechnol., 11: 379-383 (1993)].
  • BMP antagonist inhibitors are in one embodiment selected from inhibitors of the BMP antagonists SOST, noggin, chordin, gremlin, and dan [Massague and Chen, "Controlling TGF- beta signaling," Genes Dev increment 14: 627-644, 2000; Aspenberg et al., "The bone morphogenetic proteins antagonist Noggin inhibits membranous ossification,” I. Bone Miner. Res.
  • tissue-selective androgen receptor modulators of the present invention may also be combined with the polypeptide osteoprotegerin for the treatment of conditions associated with bone loss, such as osteoporosis.
  • osteoprotegerin is mammalian osteoprotegerin and more preferably human osteoprotegerin.
  • the polypeptide osteoprotegerin a member ot the tumor necrosis factor receptor superfamily, is useful to treat bone diseases characterized by increased bone loss, such as osteoporosis.
  • Prostaglandin derivatives may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Prostaglandin derivatives are in one embodiment selected from agonists of prostaglandin receptor EP1, EP2, EP4, FP and IP or a derivative thereof [Pilbeam et al., "Prostaglandins and bone metabolism,” 1996. In: Bilezikian, et al. Ed. Principles of Bone Biology, San Diego: Academic Press; Weinreb et al., “Expression of the prostaglandin E(2) (PGE(2)) receptor subtype EP(4) and its regulation by PGE(2) in osteoblastic cell lines and adult rat bone tissue," Bone, 28: 275-281 (2001)].
  • Fibroblast growth factors may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Fibroblast growth factors include aFGF, bFGF and related peptides with FGF activity [Hurley Florkiewicz, "Fibroblast growth factor and vascular endothelial growth factor families," 1996. In: T. P. Bilezikian, et al., Ed. Principles of Bone Biology, San Diego: Academic Press].
  • Vitamin D and vitamin D derivatives may also be employed together with the tissue selective androgen receptor modulator of structural formula I.
  • Vitamin D and vitamin D derivatives include natural vitamin D, 25-OH- vitamin D3, l ,25(OH)2 vitamin D3, l ⁇ -OH- vitamin D3, l ⁇ -OH-vitamin D2, dihydrotachysterol, 26,27-F6-l ⁇ ,25(OH)2 vitamin D3, 19-nor-l ⁇ ,25(OH)2 vitamin D3, 22-oxacalcitriol, calcipotriol, l ⁇ ,25(OH)2-16-ene-23-yne-vitamin D3 (Ro 23-7553), EB1089, 20-epi- l ⁇ ,25(OH)2 vitamin D3, KH1060, ED71, l ⁇ ,24(S)-(OH)2 vitamin D3, l ⁇ ,24(R)- (OH)2 vitamin D3 [See, Tones G., "Pharmacological mechanisms of therapeutics: vitamin D and analog
  • Vitamin K and vitamin K derivatives may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Vitamin K and vitamin K derivatives include menatetrenone (vitamin K2) [see Shiraki et al., "Vitamin K2 (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis," T. Bone Miner. Res., 15: 515-521 (2000)].
  • Soy isoflavones may be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Fluoride salts including sodium fluoride (NaF) and monosodium fluorophosphate (MFP), may also be employed together with the tissue selective androgen receptor modulators of structural formula I.
  • Dietary calcium supplements may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Dietary calcium supplements include calcium carbonate, calcium citrate, and natural calcium salts (Heaney. Calcium. 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press).
  • Daily dosage ranges for bone resorption inhibitors, osteoanabolic agents and other agents which may be used to benefit the skeleton when used in combination with the compounds of structural formula I are those which are known in the art.
  • the daily dosage range for the tissue selective androgen receptor modulator of structural formula I is 0.01 to 1000 mg per adult human per day, more preferably from 0.1 to 200 mg/day.
  • adjustments to decrease the dose of each agent may be made due to the increased efficacy of the combined agent.
  • 100 mg/day are appropriate for treatment, more preferably 5 to 20 mg/day, especially about 10 mg day.
  • doses of about 2.5 to about 10 mg/day and especially about 5 mg/day should be employed.
  • doses of about 15 mg to 700 mg per week of bisphosphonate and 0.07 to 7000 mg of the compound of structural formula I may be employed, either separately, or in a combined dosage form.
  • the compound of structural formula I may be favorably administered in a controlled-release delivery device, particularly for once weekly administration.
  • the compounds of structural formula I may be effectively administered in combination with one or more additional active agents.
  • the additional active agent or agents can be lipid-altering compounds such as HMG-CoA reductase inhibitors, or agents having other pharmaceutical activities, or agents that have both lipid-altering effects and other pharmaceutical activities.
  • HMG-CoA reductase inhibitors include statins in their lactonized or dihydroxy open acid forms and pharmaceutically acceptable salts and esters thereof, including but not limited to lovastatin (see US Patent No. 4,342,767); simvastatin (see US Patent No.
  • simvastatin particularly the ammonium or calcium salts thereof
  • pravastatin particularly the sodium salt thereof
  • fluvastatin particularly the sodium salt thereof
  • atorvastatin particularly the calcium salt thereof
  • cerivastatin particularly the sodium salt thereof
  • nisvastatin also referred to as NK-104 (see PCT international application publication number WO 97/23200).
  • Additional active agents which may be employed in combination with a compound of structural formula I include, but are not limited to, HMG-CoA synthase inhibitors; squalene epoxidase inhibitors; squalene synthetase inhibitors (also known as squalene synthase inhibitors), acyl- coenzyme A: cholesterol acyltransferase (ACAT) inhibitors including selective inhibitors of ACAT-1 or ACAT-2 as well as dual inhibitors of ACAT-1 and -2; microsomal triglyceride transfer protein (MTP) inhibitors; probucol; niacin; cholesterol absorption inhibitors, such as SCH-58235, also known as ezetimibe and 1- (4-fluorophenyl)-3(R)-[3(S)-(4-fluorophenyl)-3-hydroxypropyl)]-4(S)-(4- hydroxyphenyl)-2-azetidinone, which is described in U.S.
  • HMG-CoA reductase inhibitors when used in combination with the compounds of structural formula I correspond to those which are known in the art.
  • AC AT microsomal triglyceride transfer protein
  • probucol niacin
  • cholesterol absorption inhibitors including ezetimibe
  • bile acid sequestrants LDL (low density lipoprotein) receptor inducers
  • platelet aggregation inhibitors including glycoprotein JSblJla fibrinogen receptor antagonists and aspirin
  • human peroxisome proliferator activated receptor gamma (PPAR ⁇ ) agonists PPAR ⁇ agonists; PPAR ⁇ agonists; PPAR dual ⁇ / ⁇ agonists
  • vitamin B6 vitamin B12
  • folic acid anti-oxidant vitamins
  • beta-blockers angiotensin IT antagonists
  • angiotensin converting enzyme inhibitors calcium channel blockers
  • endothelin antagonists agents such as LXR ligands that enhance ABC1 gene expression; bisphosphonate compounds; and cyclooxygena
  • the individual components of the combination can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms.
  • the instant invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating diseases caused by androgen deficiency or that can be ameliorated by addition of androgen.
  • the compounds of the present invention can be prepared according to the procedures denoted in the following reaction Schemes and Examples or modifications thereof using readily available starting materials, reagents, and conventional procedures or variations thereof well-known to a practitioner of ordinary skill in the art of synthetic organic chemistry. Specific definitions of variables in the Schemes are given for illustrative purposes only and are not intended to limit the procedures described.
  • the selective androgen receptor modulators (SARMs) of structural formula L3 in Scheme 1 are prepared as follows.
  • SARMs of structural formula 2 ⁇ 3 in Scheme 2 are prepared in a similar fashion as those in Scheme 1 but using intermediates of formula 2 ⁇ having a double bond at the C1-C2 position of the 4-azasteroid "A" ring which can be prepared by treating intermediates LI with DDQ and BSTFA in toluene.
  • the SARMs of structural formula 3_ 2 in Scheme 3 are prepared as follows. Alkylation at the 16-position is effected by treatment of the enolate generated from the 17-ketone of structural formula 3-1 with an organic base such as lithium diisopropylamide (LDA) or an alkali metal hexamethyldisilazide (such as LiHMDS or NaHMDS) in a suitable organic solvent such as diethyl ether and tetrahydrofuran with an electrophile R5 ⁇ r or R5 ⁇ Tf.
  • an organic base such as lithium diisopropylamide (LDA) or an alkali metal hexamethyldisilazide (such as LiHMDS or NaHMDS)
  • LDA lithium diisopropylamide
  • NaHMDS alkali metal hexamethyldisilazide
  • the derived 16-alkylated-17- ketone intermediate is then reduced with a suitable reducing agent such as sodium borohydride in a suitable organic solvent to afford stereoselectively the desired 16R5- alkylated 17 ⁇ -ol compounds of structural formula 3-2.
  • a suitable reducing agent such as sodium borohydride in a suitable organic solvent
  • the SARMs of structural formula 4 ⁇ 2 in Scheme 4 can be prepared as follows. A mixed aldol condensation between 17-ketone 3 ⁇ and an aldehyde or ketone of formula 4-1 affords an ⁇ , ⁇ -unsaturated 17-ketone intermediate which is subjected to 1,2-carbonyl reduction in a stereoselective fashion with a suitable reducing reagent such as sodium borohydride in a suitable organic solvent.
  • a suitable reducing reagent such as sodium borohydride in a suitable organic solvent.
  • Step A 4-Methyl-4-aza-5 ⁇ -androstan- 17-one (5-2)
  • Step B 4, 17 ⁇ -Dimethyl- 17 ⁇ -hvdroxy-4-aza-5 ⁇ -androstan-3-one (5-3) To 5 2 (8.0 g, 26.36 mmol) in THF (130 mL) at -78°C was added
  • Step C 4, 17 ⁇ -Dime ⁇ hyl- 17 ⁇ -hydroxy-4-aza-5 ⁇ -androst- 1 -en-3 -one (5-6)
  • Step A 17 ⁇ -E ⁇ hvnyl-l 7 ⁇ -hvdroxy-4-methyl-4-aza-5 ⁇ -androstan-3-one (5-4)
  • Step B 17 ⁇ -Ethyl-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androstan-3-one (5-5)
  • Step B 4- Aza-5 ⁇ -androst- 1 -en-3, 17-dione (6-3)
  • Oxalyl chloride (0.98 mL, 11.3 mmol) was dissolved in CH 2 C1 2 (100 mL) and cooled to -78°C.
  • DMSO (1.67 mL, 23.5 mmol) was added and the reaction was stirred at -78 °C for 5 min.
  • Alcohol 6_ 2 (2.85 g, 9.4 mmol) was dissolved in CH 2 C1 2 (50 mL) and added to the reaction dropwise over 20 min. The reaction was stirred at -78 °C for 1 h.
  • Et 3 N (6.6 mL, 47.0 mmol) was added and the reaction was stirred at -78 °C for 1 h and then at 0 °C for 2 h.
  • Step A 16 ⁇ -(3-Fluorobenzyl)-4-methyl-4-aza-5 ⁇ -androst-l-en-3,17-dione
  • ketone 3 (0.500 g, 1.66 mmol) in 20:1 THRDMF (10 mL) at 0 °C was added dropwise a solution of lithium diisopropylamide (1.5 M in cyclohexane, 2.21 mL, 3.32 mmol). After one h, the resulting mixture was cooled to -45 °C, and 3-fluorobenzyl bromide (0.394 mL, 3.32 mmol) was slowly added. The resulting mixture was warmed to -20 °C, and after three h was quenched with water (10 mL) and diluted with CH 2 C1 (50 mL). The organic portion was separated, dried over magnesium sulfate, and evaporated.
  • Step B 16 ⁇ -(3-Fluorobenzyl)-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androst-l-en-
  • Step B 16-(3,5-Difluorobenzylidene)-17 ⁇ -hvdroxy-4-methyl-4-aza-5 ⁇ - androst-l-en-3-one (8-2)
  • Step A 16 ⁇ -Fluoro-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 , 17-dione (9- 1 )
  • Ketone 6X3 (0.163 g, 0.54 mmol) was dissolved in THF (3.0 mL) and cooled to -78°C.
  • LDA (0.47 mL of 1.5 M soln in cyclohexane, 0.70 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min.
  • Step B 16 ⁇ -Fluoro- 17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 -one (9-2) and 16 ⁇ -fluoro-17 ⁇ -hydroxy-4-mefhyl-4-aza-5 ⁇ -androst-l-en-3-one (9-3)
  • Fluoroketone 94 (0.100 g, 0.31 mmol) was dissolved in CH 2 C1 2 (2.0 mL) and cooled to -78°C.
  • DIBAL (0.34 mL of 1M solution in CH 2 C1 2 , 0.34 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h. Another 0.30 ml of DIBAL was added and reaction was stirred for 2 hours.
  • Step A 16 ⁇ -Fluoro-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 , 17-dione (9-4)
  • Fluoroketone 94 (0.115 g, 0.36 mmol) was dissolved in THF (3.0 mL) and cooled to -78°C.
  • LDA (0.31 mL of 1.5 M soln in cyclohexane, 0.47 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min.
  • Step B 16 ⁇ -Fluoro-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androst-l-en-3-one (9-5)
  • Fluoroketone 9 (0.065 g, 0.20 mmol) was dissolved in THF (2.0 mL) and cooled to -78°C.
  • LAH (0.41 mL of 1M solution in THF, 0.41 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h.
  • the reaction was quenched by the addition of MeOH (2.0 mL) at -78 °C.
  • Saturated Rochelles salt (15 mL) was added and the reaction was extracted with CH 2 C1 2 (3 x 70 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated.
  • Step A 16 ⁇ -Fluoro-4, 16 ⁇ -dimethyl- 17 ⁇ -hydroxy-5 ⁇ -androst-l -en-3-one (9-6)
  • Fluoroketone 9 (0.019 g, 0.06 mmol) was dissolved in Et 2 O and cooled to -78 °C. MeMgBr (0.06 mL of 3 M solution in Et 2 O, 0.18 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was allowed to warm to 25 °C and stirred for 18 h. The reaction was quenched by the addition of saturated aqueous NH 4 CI (5 mL) and extracted with CH 2 C1 2 (3 x 70 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes) to afford fluoroalcohol 9 ⁇ 6 (6 mg). MS (ES, M+H) calcd 336, found 336.
  • Step A 17 ⁇ -(tert-Butyldimethylsilyloxy)-4-methyl-4-aza-5 ⁇ -androstan-3 -one
  • Step B 2 ⁇ -Fluoro-17 ⁇ -(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5 ⁇ - androstan-3-one (10-2)
  • THF 5.0 ml
  • THF 5.0 ml
  • THF 5.0 ml
  • THF 1.14 ml, 1.71 mmol
  • a solution of N-fluorobenzenesulfonimide (2.25 g, 7.15 mmol) in 2 ml THF was added dropwise over 5 min. After 30 min, the cooling bath was removed and the reaction was stirred for 14 h.
  • Et 2 O was added and then washed with H 2 O, saturated aqueous NaHCO 3 , brine, dried (MgSO 4 ) and then concentrated.
  • Step C 2 ⁇ -Fluoro-l 7 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androstan-3-one (10-3)
  • THF 2.0 ml
  • LDA 2.0 M LDA
  • N-fluorobenzenesulfonimide 2.25g, 7.15 mmol
  • 1.0 ml HF was added and the mixture stirred for 30 min.
  • Step A 2 ⁇ -Fluoro-17 ⁇ -(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5 ⁇ - androstan-3-one (10-4)
  • THF tetrahydrofuran
  • a solution of 1.5 M LDA in THF 0.230 mL, 0.340 mmol
  • a solution of 0.1 mL AcOH in 2 mL THF was then added dropwise over 5 min.
  • the cooling bath was removed and the reaction was stirred for 14 h.
  • Et 2 O was added and then washed with H 2 O, saturated aqueous sodium hydrogencarbonate, brine, dried (MgSO 4 ) and then concentrated.
  • Step B 2 ⁇ -Fluoro-l 7 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androstan-3-one (10-5)
  • Step A 2-Fluoro-17 ⁇ -(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5 ⁇ -androst- l-en-3-one (ll-l)
  • Step B 2-Fluoro-l 7 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 -one (11-2)
  • Step A 16 ⁇ -Bromo-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 , 17-dione (12-1)
  • Ketone 6 3 (0.100 g, 0.33 mmol) was dissolved in /-PrOH (2.5 mL) at 25 °C.
  • CuBr 2 (0.230 g, 1.03 mmol) was added and the reaction was heated to 83 °C and stirred for 2 h.
  • the reaction was quenched by the addition of saturated aqueous sodium hydrogencarbonate solution (5 mL) and extracted with CH 2 C1 2 (3 x 70 mL). The combined organic layers were dried with NaSO 4 , filtered and concentrated. The residue was purified by flash chromatography on silica gel (80-100% EtOAc in hexanes as eluent) to afford keto bromide 12-1 (0.115 g).
  • MS (ES, M+H) calcd 380, found 380.
  • Step B 16 ⁇ -Hydroxy-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 , 17-dione (12-2)
  • Ketobromide 124 (0.028 g, 0.07 mmol) was dissolved in THF (1.0 mL) at 25 °C. 40% aq KOH (1.0 mL) was added and the reaction was stirred 18 h at room temperature. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH 2 C1 2 (3 x 50 mL). The combined organic layers were dried with Na 2 SO 4 , filtered and concentrated. The residue was purified by flash chromatography on silica gel (100% EtOAc as eluent) to afford hydroxyketone 12-2 (0.022 g). MS (ES, M+H) calcd 318, found 318.
  • Step C 16 ⁇ , 17 ⁇ -Dihvdroxy-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 -one (12-3)
  • Step A: 16 ⁇ -Methoxy-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 , 17-dione (12-4) Hydroxyketone 12 2 (0.020 g, 0.06 mmol) was dissolved in DMF (1.0 ml) and cooled to 0 °C. NaH (0.002 g, 0.09 mmol) was added and the reaction was stirred at 0 °C for 5 min. Iodomethane (0.01 mL, 0.16 mmol) was added and the reaction was warmed to room temperature and stirred for 3 h. The reaction was quenched by the addition of saturated aqueous NH 4 CI (2 mL) and extracted with CH 2 C1 2 (3 x 50 mL).
  • Step B 17 ⁇ -Hydroxy-16 ⁇ -methoxy-4-methyl-4-aza-5 ⁇ -androst-l-en-3-one
  • Step A 16 ⁇ -Morpholino-4-methyl-4-aza-5 ⁇ -androst-l -en-3 , 17-dione (13-1)
  • Ketone 24 (0.020 g, 0.05 mmol) was dissolved in MeCN (1 mL). Morpholine (0.01 mL, 0.054 mmol) was added and the reaction was stirred for 36 h. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH 2 C1 2 (3 x 50 mL). The combined organic layers were dried with Na 2 SO , filtered and concentrated. The residue was purified by flash chromatography on silica gel (90- 100% EtOAc in hexanes as eluent) to afford ketone 13-1 (4 mg). MS (ES, M+H) calcd 387, found 387.
  • Step B 17 ⁇ -Methyl- 16 ⁇ -morpholino-4-methyl-4-aza-5 ⁇ -androst- 1 -en-3 -one
  • Step A 16 ⁇ -(Ethyloxycarbonylmethyl)-4-methyl-4-aza-5 ⁇ -androst-l-en-3,17- dione (14-1)
  • Ketone 6 3 (0.150 g, 0.50 mmol) was dissolved in THF (5.0 mL) and cooled to -78°C.
  • LDA (0.47 mL of 1.5 M soln in cyclohexane, 0.65 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min. The reaction was then recooled to -78 °C and ethyl iodoacetate (0.09 mL, 0.75 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h and then warmed to 0 °C and stirred for 15 min.
  • Step B 16 ⁇ -(Ethyloxycarbonylmethyl)-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ - androst-1 -en-3 -one (14-2)
  • Ketone 144 (0.038 g, 0.13 mmol) was dissolved in MeOH CH 2 Cl 2 3:1 (3 mL) and cooled to 0 °C.
  • Step A 16 ⁇ -(Carboxymethyl)-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ -androst-l-en-3- one (14-3)
  • Ester 14-2 (0.020 g, 0.05 mmol) was dissolved in MeOH (1.0 mL) at 25 °C. 40% aqueous KOH (0.5 mL) was added and the reaction was stirred 18 h at room temperature. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH 2 C1 2 (3 x 50 mL). The combined organic layers were dried with Na 2 SO , filtered and concentrated to afford crude acid 14-3 (0.019 g). MS (ES, M+H) calcd 362, found 362).
  • Step B 16 ⁇ -(Benzylaminocarbonylmethyl)-17 ⁇ -hydroxy-4-methyl-4-aza-5 ⁇ - androst-l-en-3-one (14-4)
  • an oral composition of a compound of this invention 50 mg of a compound of the present invention is formatted with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size 0 hard gelatin capsule.
  • the silicone fluid and compound of structural formula I are mixed together and the colloidal silicone dioxide is added to increase viscosity.
  • the material is then dosed into a subsequently heat sealed polymeric laminate comprised of the following: polyester release liner, skin contact adhesive composed of silicone or acrylic polymers, a control membrane which is a polyolefin (e.g. polyethylene, polyvinyl acetate or polyurethane), and an impermeable backing membrane made of a polyester multilaminate.
  • the resulting laminated sheet is then cut into 10 cm ⁇ patches. For 100 Patches.
  • the polyethylene glycol 1000 and polyethylene glycol 4000 are mixed and melted.
  • the compound of structural formula I is mixed into the molten mixture, poured into molds and allowed to cool. For 1000 suppositories.
  • the compound of structural formula I, magnesium sulfate heptahydrate and buffering agents are dissolved in the water for injection with stirring, and the resulting solution is filtered, filled into ampoules, sealed and sterilized by autoclaving. For 1000 Ampoules.
  • tissue selective androgen receptor modulators of the present invention were used to characterize the activity of the tissue selective androgen receptor modulators of the present invention.
  • Binding Buffer 10 mM Tris-HCl, 1 mM EDTA, 10% glycerol, 1 mM beta- mecaptoethanol, 10 mM Sodium Molybdate, pH 7.2
  • HAP Slurry Calbiochem Hydroxylapatite, Fast Flow, in 10 mM Tris, pH 8.0 and l mM EDTA. Wash Buffer: 40 mM Tris, pH7.5, 100 mM KCl, 1 mM EDTA and 1 mM EGTA.
  • DHT Dihydrotestosterone
  • NEN NET453 Hydroxylapatite Fast Flow Calbiochem Cat#391947
  • Molybdate Molybdic Acid (Sigma, M1651)
  • MDA-MB-453 cell culture media
  • the MDA cells When the MDA cells are 70 to 85% confluent, they are detached as described above, and collected by centrifuging at 1000 g for 10 min at 4°C. The cell pellet is washed twice with TEGM (10 mM Tris-HCl, 1 mM EDTA, 10% glycerol, 1 mM beta-mercaptoethanol, 10 mM Sodium Molybdate, pH 7.2). After the final wash, the cells are resuspended in TEGM at a concentration of 107 cells/mL. The cell suspension is snap frozen in liquid nitrogen or ethanol/dry ice bath and transferred to -80°C freezer on dry ice.
  • TEGM 10 mM Tris-HCl, 1 mM EDTA, 10% glycerol, 1 mM beta-mercaptoethanol, 10 mM Sodium Molybdate, pH 7.2
  • the frozen samples are left on ice-water to just thaw ( ⁇ 1 hr). Then the samples are centrifuged at 12,500 g to 20,000 g for 30 min at 4°C. The supernatant is used to set-up assay right away. If using 50 ⁇ L of supernatant, the test compound can be prepared in 50 ⁇ L of the TEGM buffer.
  • Procedure for Multiple Compound Screening lx TEGM buffer is prepared, and the isotope-containing assay mixture is prepared in the following order: EtOH (2% final concentration in reaction), 3H- R1881 or 3H-DHT (0.5 nM final Cone, in reaction) and lx TEGM. [eg.
  • the HAP pellet on the filter plate is incubated with 50 ⁇ L of MTCROSCINT (Packard) scintillint for 30 minutes before being counted on the TopCount microscintillation counter (Packard).
  • IC50S are calculated using R1881 as a reference.
  • Tissue selective androgen receptor modulators of the present invention displayed IC50 values of 1 micromolar or less.
  • MMPl Promoter Suppression, Transient Transfection Assay HepG2 cells are cultured in phenol red free MEM containing 10 % charcoal-treated FCS at 37C with 5% CO2. For transfection, cells are plated at 10,000 cells/well in 96 well white, clear bottom plates. Twenty four hours later, cells are co-transfected with a MMPl promoter-lucif erase reporter construct and a rhesus monkey expression construct (50 : 1 ratio) using FuGENE ⁇ transfection reagent, following the protocol recommended by manufacturer.
  • TRAMPS Transient Transfection Assay
  • the MMPl promoter-lucif erase reporter construct is generated by insertion of a human MMPl promoter fragment (-179/+63) into pGL2 luciferase reporter construct (Promega) and a rhesus monkey AR expression construct is generated in a CMV-Tag2B expression vector (Stratagene). Cells are further cultured for 24 hours and then treated with test compounds in the presence of 100 nM phorbol-12-myristate-13-acetate (PMA), used to increase the basal activity of MMPl promoter.
  • PMA phorbol-12-myristate-13-acetate
  • the compounds are added at this point, at a range of lOOOnM to 0.03nM, 10 dilutions, at a concentration on 10X, 1/lOth volume (example: 10 microliters of ligand at 10X added to 100 microliters of media already in the well).
  • Cells are further cultured for an additional 48 hours.
  • Cells are then washed twice with PBS and lysed by adding 70 ⁇ L of Lysis Buffer (lx, Promega) to the wells.
  • Lysis Buffer lx, Promega
  • the luciferase activity is measured in a 96-well format using a 1450 Microbeta let (Perkin Elmer) luminometer. Activity of test compounds is presented as suppression of luciferase signal from the PMA-stimulated control levels. EC50 and Emax values are reported.
  • Tissue selective androgen receptor modulators of the present invention activate repression typically with submicromolar EC50 values and Emax values greater than about 50%.
  • This assay assesses the ability of AR agonists to induce the interaction between the N-terminal domain (NTD) and C-terminal domain (CTD) of rhAR that reflects the in vivo virilizing potential mediated by activated androgen receptors.
  • NTD N-terminal domain
  • CTD C-terminal domain
  • the interaction of NTD and CTD of rhAR is quantified as ligand induced association between a Gal4DBD-rhARCTD fusion protein and a VP16-rhARNTD fusion protein as a mammalian two-hybrid assay in CV-1 monkey kidney cells.
  • CV-1 cells are trypsinized and counted, and then plated at 20,000 cells/well in 96-well plates or larger plates (scaled up accordingly) in DMEM + 10% FCS.
  • CV-1 cells are cotransfected with pCBBl (Gal4DBD-rhARLBD fusion construct expressed under the SV40 early promoter), pCBB2 (VP16 -rhAR NTD fusion construct expressed under the SV40 early promoter) and pFR (Gal4 responsive luciferase reporter, Promega) using LTJPOFECTAMINE PLUS reagent (GTBCO-BRL) following the procedure recommended by the vendor.
  • pCBBl Gal4DBD-rhARLBD fusion construct expressed under the SV40 early promoter
  • pCBB2 VP16 -rhAR NTD fusion construct expressed under the SV40 early promoter
  • pFR Gal4 responsive luciferase reporter, Promega
  • DNA admixture of 0.05 ⁇ g pCBBl, 0.05 ⁇ g pCBB2 and 0.1 ⁇ g of pFR is mixed in 3.4 ⁇ L OPTI-MEM (GIBCO-BRL) mixed with "PLUS Reagent" (1.6 ⁇ L, GIBCO-BRL) and incubated at room temperature (RT) for 15 min to form the pre-complexed DNA.
  • LTPOFECTAMINE Reagent GTBCO-BRL
  • OPTI-MEM phosphatidylcholine
  • the pre-complexed DNA (above) and the diluted LIPOFECTAMTNE Reagent (above) are combined, mixed and incubated for 15 min at RT.
  • the medium on the cells is replaced with 40 ⁇ L /well OPTI-MEM, and 10 ⁇ L DNA-lipid complexes are added to each well.
  • the complexes are mixed into the medium gently and incubated at 37°C at 5% CO2 for 5h.
  • test compounds are added at the desired concentration(s) (1 nM - 10 ⁇ M). Forty eight hours later, luciferase activity is measured using LUC-Screen system (TROPIX) following the manufacturer's protocol.
  • the assay is conducted directly in the wells by sequential addition of 50 ⁇ L each of assay solution 1 followed by assay solution 2. After incubation for 40 minutes at room temperature, luminescence is directly measured with 2-5 second integration. Activity of test compounds is calculated as the E m a ⁇ relative to the activity obtained with 3 nM R1881. Typical tissue-selective androgen receptor modulators of the present invention display weak or no agonist activity in this assay with less than 50% agonist activity at 10 micromolar.
  • Terminus and C-Terminus Domains of Androgen Receptor (Antagonist Mode) This assay assesses the ability of test compounds to antagonize the stimulatory effects of Rl 881 on the interaction between NTD and CTD of rhAR in a mammalian two-hybrid assay in CV-1 cells as described above.
  • CV-1 cells are treated with test compounds , typically at 10 ⁇ M, 3.3 ⁇ M, 1 ⁇ M, 0.33 ⁇ M, 100 nM, 33 nM, 10 nM, 3.3 nM and 1 nM final concentrations.
  • test compounds typically at 10 ⁇ M, 3.3 ⁇ M, 1 ⁇ M, 0.33 ⁇ M, 100 nM, 33 nM, 10 nM, 3.3 nM and 1 nM final concentrations.
  • an AR agonist methyltrienolone R1881
  • luciferase activity is measured using LUC-Screen system (TROPLX) following the protocol recommended by the manufacturer.
  • the ability of test compounds to antagonize the action of R1881 is calculated as the relative luminescence compared to the value with 0.3 nM R1881 alone.
  • SARM compounds of the present invention typically displayed antagonist activity in the present assay with IC50 values less than 1 micromolar.
  • TAMAR Trans- Activation Modulation of Androgen Receptor
  • 20,000 to 30,000 cells/well are plated in a white, clear-bottom 96-well plate in "Exponential Growth Medium” which consists of phenol red-free RPMI 1640 containing 10%FBS, 4mM L-glutamine, 20mM HEPES, lOug/mL human insulin, and 20ug/mL gentamicin. Incubator conditions are 37°C and 5% CO 2 . The transfection is done in batch mode. The cells are trypsinized and counted to the right cell number in the proper amount of fresh media, and then gently mixed with the Fugene/DNA cocktail mix and plated onto the 96-well plate.
  • "Exponential Growth Medium” which consists of phenol red-free RPMI 1640 containing 10%FBS, 4mM L-glutamine, 20mM HEPES, lOug/mL human insulin, and 20ug/mL gentamicin. Incubator conditions are 37°C and 5% CO 2 . The transfection
  • the transfection cocktail consists of serum-free Optimem, Fugene ⁇ reagent and DNA. The manufacturer's (Roche Biochemical) protocol for cocktail setup is followed. The lipid ( ⁇ l) to DNA ( ⁇ g) ratio is approximately 3:2 and the incubation time is 20 min at room temperature. Sixteen to 24 hrs after transfection, the cells are treated with test compounds such that the final DMSO (vehicle) concentration is ⁇ 3%. The cells are exposed to the test compounds for 48 hrs.
  • the cells are lysed by a Promega cell culture lysis buffer for 30-60 min and then the luciferase activity in the extracts is assayed in the 96-well format luminometer. Activity of test compounds is calculated as the E m ax relative to the activity obtained with 100 nM R1881.
  • the spermatic artery and vas deferens are ligated with 4.0 silk 0.5cm proximal to the testicle.
  • the testicle is freed by one cut of a small surgical scissors distal to the ligation site.
  • the tissue stump is returned to the scrotum.
  • the same is repeated for the left testicle.
  • both stumps are returned to the scrotum, the scrotum and overlying skin are sutured closed with 4.0 silk.
  • Sham-ORX all procedures excepting ligation and scissors cutting are completed.
  • the rats fully recover consciousness and full mobility within 10-15 minutes.
  • a dose of test compound is administered subcutaneously or orally to the rat immediately after the surgical incision is sutured. Treatment continues for an additional six consecutive days.
  • Necropsy and Endpoints The rat is first weighed, then anesthetized in a CO2 chamber until near death. Approximately 5ml whole blood is obtained by cardiac puncture. The rat is then examined for certain signs of death and completeness of ORX. Next, the ventral portion of the prostate gland is located and blunt dissected free in a highly stylized fashion. The ventral prostate is blotted dry for 3-5 seconds and then weighed (VPW). Finally, the seminal vesicle is located and dissected free. The ventral seminal vesicle is blotted dry for 3-5 seconds and then weighed (SVWT).
  • Primary data for this assay are the weights of the ventral prostate and seminal vesicle. Secondary data include serum LH (luteinizing hormone) and FSH (follicle stimulating hormone), and possible serum markers of bone formation and virilization. Data are analyzed by ANOVA plus Fisher PLSD post-hoc test to identify intergroup differences. The extent to which test compounds inhibit ORX-induced loss of VPW and SVWT is assessed.
  • the rat is first weighed, then anesthetized in a CO2 chamber until near death. Approximately 5mL whole blood is obtained by cardiac puncture. The rat is then examined for certain signs of death and completeness of OVX. First, the uterus is located, blunt dissected free in a highly stylized fashion, blotted dry for 3-5 seconds and then weighed (UW). The uterus is placed in 10% neutral-buffered formalin. Next, the right leg is disarticulated at the hip. The femur and tibia are separated at the knee, substantially defleshed, and then placed in 70% ethanol.
  • One section from each rat that approximates the midpoint of the bone is selected and blind-coded.
  • the periosteal surface of each section is assessed for total periosteal surface, single fluorochrome label, double fluorochrome label, and interlabel distance.

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Abstract

Compounds of structural formula I as herein defined are disclosed as useful in a method for modulating the androgen receptor in a tissue selective manner in a patient in need of such modulation, as well as in a method of activating the function of the androgen receptor in a patient, and in particular the method wherein the function of the androgen receptor is blocked in the prostate of a male patient or in the uterus of a female patient and activated in bone and/or muscle tissue. These compounds are useful in the treatment of conditions caused by androgen deficiency or which can be ameliorated by androgen administration, including osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, female sexual dysfunction, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, pancreatic cancer, renal cancer, prostate cancer, arthritis and joint repair, alone or in combination with other active agents.

Description

17-HYDROXY-4-AZA-ANDROSTAN-3-ONES AS ANDROGEN RECEPTOR MODULATORS
BACKGROUND OF THE INVENTION
The androgen receptor (AR) belongs to the superfamily of steroid thyroid hormone nuclear receptors, whose other members include the estrogen receptor (ER), the progesterone receptor (PR), the glucocorticoid receptor (GR), and the mineralocorticoid receptor (MR). The AR is expressed in numerous tissues of the body and is the receptor through which the physiological as well as the pathophysiological effects of endogenous androgen ligands, such as testosterone (T) and dihydrotestosterone (DHT), are expressed. Structurally, the AR is composed of three main functional domains: the ligand binding domain (LBD), the DNA-binding domain, and amino-terminal domain. A compound that binds to the AR and mimics the effects of an endogenous AR ligand is referred to as an AR agonist, whereas a compound that inhibits the effects of an endogenous AR ligand is termed an AR antagonist.
Androgen ligand binding to the AR affords a ligand/receptor complex, which, subsequent to translocation inside the nucleus of the cell, binds to specific regulatory DNA sequences (referred to as androgen response elements or AREs) within the promoter or enhancer regions of the target gene or genes present in the cell's nucleus. Other proteins termed cof actors are next recruited which bind to the amino-terminal domain or the LBD of the receptor leading to gene transcription and subsequent translation to produce the protein(s) encoded by that gene or genes. Androgen therapy has been used in the clinic to treat a variety of male disorders, such as reproductive disorders and primary or secondary male hypogonadism. Moreover, a number of natural or synthetic AR agonists have been clinically investigated for the treatment of musculoskeletal disorders, such as bone disease, hematopoietic disorders, neuromuscular disease, rheumatological disease, wasting disease, and for hormone replacement therapy (HRT), such as female androgen deficiency. In addition, AR antagonists, such as flutamide and bicalutamide, have been used to treat prostate cancer. It would therefore be useful to have available compounds that can activate ("agonize") the function of the AR in a tissue-selective manner which would afford the desired beneficial osteo- and myoanabolic effects of androgens but without the negative androgenic properties, such as virilization and induction of an atherogenic lipid profile which can lead to cardiovascular disease.
The role of androgens in bone formation has been documented. For example, anabolic steroids, such as nandrolone decanoate or stanozolol, have been shown to increase bone mass in postmenopausal women. The beneficial effects of androgens on bone in postmenopausal osteoporosis were documented in recent studies using combined testosterone and estrogen administration [Hofbauer, et al., " Androgen effects on bone metabolism: recent progress and controversies," Eur. J. Endocrinol.140: 271-286 (1999)]. Combined treatment significantly increased the rate and extent of the rise in bone mineral density (BMD) in the lumbar and hip regions, relative to treatment with estrogen alone. Additionally, estrogen - progestin combinations that incorporated an androgenic progestin (such as norethindrone), rather than medroxyprogesterone acetate, yielded greater improvements in hip BMD. These results have recently been confirmed in a larger 2-year, double-blind comparison study in which oral conjugated estrogen (CEE) and methyltestosterone combinations were demonstrated to be effective in promoting accrual of bone mass in the spine and hip, while conjugated estrogen therapy alone prevented bone loss ["A two-year, double-blind comparison of estrogen-androgen and conjugated estrogens in surgically menopausal women: Effects on bone mineral density, symptoms and lipid profiles," J. Reprod. Med., 44: 1012-1020 (1999)]. Despite the beneficial effects of androgens in postmenopausal women, the use of androgens has been limited because of the undesirable virilizing and metabolic action of androgens. The data from Watts and colleagues demonstrate that hot flushes decrease in women treated with CEE and methyltestosterone; however, 30% of these women suffered from significant increases in acne and facial hair, a complication of all current androgen pharmacotherapies [Watts, et al., "Comparison of oral estrogens and estrogens plus androgen on bone mineral density, menopausal symptoms, and lipid-lipoprotein profiles in surgical menopause," Obstet. Gvnecol., 85: 529-537 (1995)]. Moreover, the addition of methyltestosterone to CEE markedly decreased FfDL levels, as seen in other studies. Therefore, non-tissue selective AR agonists may increase the risk of cardiovascular disease. Thus, the virilizing potential and negative effects on lipid profile of current androgen therapies provide a strong rationale for developing tissue-selective androgen receptor agonists for bone. Reference is made to J. A. Kanis, "Other agents for generalized osteoporosis," in Osteoporosis, Blackwell Science, Ch. 8, pp 196-227 (1994) for a discussion of non-selective anabolic steroids in the treatment of osteoporosis.
It is also well established that androgens play an important role in bone metabolism in men, which parallels the role of estrogens in women [Anderson, et al., "Androgen supplementation in eugonadal men with osteoporosis - effects of six months of treatment on bone mineral density and cardiovascular risk factors," Bone, 18: 171-177 (1996)]. Even in eugonadal men with established osteoporosis, the therapeutic response to testosterone treatment provided additional evidence that androgens exert important osteoanabolic effects. Mean lumbar BMD increased from 0.799 gm/cm2 to 0.839 g/cm2, in 5 to 6 months in response to 250 mg of testosterone ester administered intramuscularly every fortnight. A common scenario for androgen deficiency occurs in men with stage D prostate cancer (metastatic) who undergo androgen deprivation therapy (ADT). Endocrine orchiectomy is achieved by long acting GnRH agonists, while androgen receptor blockade is implemented with flutamide, nilutamide, bicalutamide, or RU 58841 (AR antagonists). In response to hormonal deprivation, these men suffered from hot flushes, significant bone loss, weakness, and fatigue. In a recent pilot study of men with stage D prostate cancer, osteopenia (50% vs. 38%) and osteoporosis (38% vs. 25%) were more common in men who had undergone ADT for greater than one year than the patients who did not undergo ADT [Wei, et al., "Androgen deprivation therapy for prostate cancer results in significant loss of bone density," Urology, 54: 607-611 (1999)]. Lumbar spine BMD was significantly lower in men who had undergone ADT. Thus, in addition to the use of tissue selective AR agonists for osteoporosis, tissue selective AR antagonists in the prostate that lack antagonistic action in bone and muscle may be useful agents for the treatment of prostate cancer, either alone or as an adjunct to traditional ADT such as with a GnRH agonist/antagonist [See also A. Stoch, et al., J. Clin. Endocrin. Metab., 86: 2787-2791 (2001)]. Tissue-selective AR antagonists may also have utility in the treatment of polycystic ovarian syndrome in postmenopausal women [see C.A. Eagleson, et al., "Polycystic ovarian syndrome: evidence that flutamide restores sensitivity of the gonadotropin-releasing hormone pulse generator to inhibition by estradiol and progesterone," J. Clin. Endocrinol. Metab., 85: 4047- 4052 (2000) and E. Diamanti-Kandarakis, "The Effect of a Pure Antiandrogen Receptor Blocker, Flutamide, on the Lipid Profile in the Polycystic Ovary Syndrome," Int. J. Endocrinol. Metab., 83: 2699-2705 (1998). There is a need for more effective agents to treat osteopenia and osteoporosis in both men and women. Osteoporosis is characterized by bone loss, resulting from an imbalance between bone resorption (destruction) and bone formation, which starts in the fourth decade and continues throughout life at the rate of about 1-4% per year [Eastell, "Treatment of postmenopausal osteoporosis," New Engl. J. Med., 338: 736 (1998)]. In the United States, there are currently about 20 million people with detectable fractures of the vertebrae due to osteoporosis. In addition, there are about 250,000 hip fractures per year due to osteoporosis, associated with a 12%-20% mortality rate within the first two years, while 30% of patients require nursing home care after the fracture and many never become fully ambulatory again. In postmenopausal women, estrogen deficiency leads to increased bone resorption resulting in bone loss in the vertebrae of around 5% per year, immediately following menopause. Thus, first line treatment/prevention of this condition is inhibition of bone resorption by bisphosphonates, estrogens, selective estrogen receptor modulators (SERMs), and calcitonin. However, inhibitors of bone resorption are not sufficient to restore bone mass for patients who have already lost a significant amount of bone. The increase in spinal BMD attained by bisphosphonate treatment can reach 11% after 7 years of treatment with alendronate. In addition, as the rate of bone turnover differs from site to site, higher in the trabecular bone of the vertebrae than in the cortex of the long bones, the bone resorption inhibitors are less effective in increasing hip BMD and preventing hip fracture. Therefore, osteoanabolic agents, which increase cortical bone formation and bone mass of long bones by stimulating periosteal bone formation, would address an unmet need in the treatment of osteoporosis especially for patients with high risk of hip fractures. The osteoanabolic agents also complement the bone resorption inhibitors that target the trabecular envelope, leading to a biomechanically favorable bone structure (Schmidt, et al., "Anabolic steroid: Steroid effects on bone in women," In: J. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press, 1996). Tissue-selective AR agonists with diminished deleterious effects on the cardiovascular system and limited virilizing potential may be useful as a monotherapy for the prevention and/or treatment of female osteoporosis. In addition, a compound with osteoanabolic properties in bone and muscle but with reduced activity in the prostate and sex accessory tissues may be useful for the prevention and/or treatment of male osteoporosis and osteopenia in men, particularly elderly men. Selective androgen receptor modulators may also be useful to treat certain hematopoietic disorders. It is known that androgens stimulate renal hypertrophy and erythropoietin (EPO) production. Prior to the introduction of recombinant human EPO, androgens were employed to treat anemia caused by chronic renal failure. In addition, androgens at pharmacological doses were found to increase serum EPO levels in anemic patients with non-severe aplastic anemia and myelodysplastic syndromes but not in non-anemic patients. Treatment modalities for anemia will require selective action such as may be provided by selective androgen receptor modulators. Furthermore, selective androgen receptor modulators may also have clinical value as an adjunct to the treatment of obesity. This approach to lowering body fat is supported by published observations that androgen administration reduced subcutaneous and visceral abdominal fat in obese men [J.C. Lovejoy, et al., "Oral anabolic steroid treatment, but not parenteral androgen treatment, decreases abdominal fat in obese, older men," Int. J. Obesity, 19: 614-624 (1995)]. Therefore, S ARMs devoid of androgenic effects on prostate may be beneficial in the treatment of obese men. In a separate study, androgen administration resulted in loss of subcutaneous abdominal fat in obese postmenopausal women [ J.C. Lovejoy, et al., "Exogenous Androgens Influence Body Composition and Regional Body Fat Distribution in Obese Postmenopausal Women - A Clinical Research Center Study," J. Clin. Endocrinol. Metab., 81: 2198-2203 (1996)]. In the latter study, nandrolone decanoate, a weak androgen and anabolic agent, was found to increase lean body mass and resting metabolic rate in obese postmenopausal women consuming a weight- reducing diet. Non-steroidal compounds having androgen receptor modulating properties were disclosed in U.S. Patent Nos. 5,688,808; 5,696,130; 6,017,924; 6,093,821; WO 01/16139 (published 8 March 2001); and WO 01/16108 (published 8 March 2001), all assigned to Ligand Pharmaceuticals, and in WO 01/27086, assigned to Kaken Pharm. Co. Additional background for the rationale behind the development of Selective Androgen Receptor Modulators is found in L. Zhi and E. Martinborough in Ann. Rep. Med. Chem. 36: 169-180 (2001). Non-steroidal SARMs were disclosed in J.P. Edwards, "New Nonsteroidal Androgen Receptor Modulators Based on 4-(Trifluoromethyl)-2(lH)-Pyrrolidino[3,2-g]quinolinone," Bioorg. Med. Chem. Lett., 8: 745-750 (1998) and in L. Zhi et al., "Switching Androgen Receptor Antagonists to Agonists by Modifying C-ring Substituents on Piperidino[3,4- g]quinoIinone," Bioorg. Med. Chem. Lett., 9: 1009-1012 (1999).
There exists a need in the clinical art for more effective agents that can elicit the positive responses of androgen replacement therapy but without the undesired side effects of non-tissue selective agonists of the AR. What is needed are compounds that can produce the same positive responses as androgen replacement therapy but without the undesired side effects. Also needed are androgenic compounds that exert selective effects on different tissues of the body. In this invention, we have identified compounds that function as selective androgen receptor modulators (SARMs) using a series of in vitro cell-assays that profile ligand mediated activation of AR, such as (i) N-C interaction, (ii) transcriptional repression, and (iii) transcriptional activation. SARM compounds in this invention, identified with the methods listed above, exhibit tissue selective AR agonism in vivo, i.e. agonism in bone (stimulation of bone formation in a rodent model of osteoporosis) and antagonism in prostate (minimal effects on prostate growth in castrated rodents and antagonism of prostate growth induced by AR agonists).
The compounds of the present invention identified as SARMs are useful to treat diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration. Such compounds are ideal for the treatment of osteoporosis in women and men as a monotherapy or in combination with inhibitors of bone resorption, such as bisphosphonates, estrogens, SERMs, cathepsin K inhibitors, αvβ3 integrin receptor antagonists, calcitonin, and proton pump inhibitors. They can also be used with agents that stimulate bone formation, such as parathyroid hormone or analogs thereof. The SARM compounds of the present invention may also be employed for treatment of prostate disease, such as prostate cancer and benign prostatic hyperplasia (BPH). Moreover, compounds of this invention exhibit minimal effects on skin (acne and facial hair growth) and may be useful for treatment of hirsutism. Additionally, compounds of this invention can stimulate muscle growth and may be useful for treatment of sarcopenia and frailty. They can be employed to reduce subcutaneous and visceral abdominal fat in the treatment of obesity. Moreover, compounds of this invention can exhibit androgen agonism in the central nervous system and may be useful to treat vasomotor symptoms (hot flush) and to increase energy and libido, particularly in postmenopausal women. The compounds of the present invention may be used in the treatment of prostate cancer, either alone or as an adjunct to traditional GnRH agonist/antagonist therapy, for their ability to restore bone, or as a replacement for antiandrogen therapy because of their ability to antagonize androgen in the prostate, and minimize bone depletion in the skeletal system. Further, the compounds of the present invention may be used for their ability to restore bone in the treatment of pancreatic cancer as an adjunct to treatment with antiandrogen, or as monotherapy for their antiandrogenic properties, offering the advantage over traditional antiandrogens of being bone-sparing. Additionally, compounds of this invention can increase the number of blood cells, such as red blood cells and platelets, and may be useful for the treatment of hematopoietic disorders, such as aplastic anemia. Finally, compounds of this invention have minimal effects on lipid metabolism. Thus, considering their tissue selective androgen receptor agonism listed above, the compounds of this invention are ideal for hormone replacement therapy in hypogonadic (androgen deficient) men.
It is therefore an object of the present invention to provide 17-hydroxy- 4-aza-androstan-3-one derivatives which are useful as selective androgen receptor modulators.
It is another object of the present invention to provide pharmaceutical compositions comprising the compounds of the present invention in association with a pharmaceutically acceptable carrier. It is another object of the present invention to provide pharmaceutical compositions comprising the steroid derivatives for use as selective androgen receptor modulators.
It is another object of the present invention to provide methods for the treatment of diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration.
It is another object of the present invention to provide methods for the treatment of diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration in combination with other agents.
It is another object of the present invention to provide 17-hydroxy-4- aza-androstan-3-one derivatives of the present invention and their pharmaceutical compositions for use as a medicament for the treatment of diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration.
It is another object of the present invention to provide 17-hydroxy-4- aza-androstan-3-one derivatives derivatives of the present invention and their pharmaceutical compositions for the manufacture of a medicament for the treatment of diseases or conditions caused by androgen deficiency which can be ameliorated by androgen administration.
These and other objects will become readily apparent from the detailed description which follows.
SUMMARY OF THE INVENTION
The present invention provides a method for modulating a function mediated by the androgen receptor in a tissue selective manner in a patient in need of such modulation, comprising administering to the patient a therapeutically effective amount of a compound of structural formula I:
Figure imgf000009_0001
(I)
or a pharmaceutically acceptable salt thereof; wherein
"a" represents a single bond or a double bond;
Rl is hydrogen, hydroxymethyl, or Ci-3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Ci_4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Cι_4 alkyl when "a" represents a single bond;
R3 is hydrogen or Cι_3 alkyl;
R4 is hydrogen, Cl-4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Cι_8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, C -4 alkoxyCi-4 alkoxy, and Cι_3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Cι_6 alkoxy,
(h) Ci-6 alkylcarbonyloxy,
(i) Ci_6 alkylthio,
(j) Ci-6 alkylsulfonyl,
(k) C3_8 cycloalkyl Cθ-6 alkyl,
(1) C3-8 cycloheteroalkyl Cθ-6 alkyl,
(m) aryl Co-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino,
(p) Ci-3 acylamino,
(q) aryl Ci-3 acylamino,
(r) Cι_6 alkylamino,
(s) di(Cχ-6 alkyl)amino,
(t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino,
(v) C3_6 cycloalkyl Cθ-2 alkylamino,
(w) Ci-8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Cι_8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Ci-8 alkylaminocarbonylamino, (cc) aryl Cø-3 alkylaminocarbonylamino, (dd) Cχ_8 alkylaminocarbonyloxy, and (ee) aryl Cø-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCQ-4 alkyl; or R5 and R6 taken together can be =CR9R10; wherein R9 and RlO are each independently selected from the group consisting of hydrogen, C - alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCl_4 alkyl, and NCι_4 alkyloxycarbonyl; wherein the aryl group in all instances above is selected from the group consisting of
(1) phenyl,
(2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl,
(6) benzoxazolyl,
(7) benzothiazolyl,
(8) benzodihydrofuranyl,
(9) 1,3-benzodioxolyl,
(10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl,
(16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl,
(21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl,
(26) oxadiazolyl,
(27) triazolyl, and (28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Ci_8 alkyl, C3-8 cycloalkyl, C3-.8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Ci_6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Cι_6 alkylsulfonyl, aryl Cθ-6aιkylsulfonyl, C\- alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cι_6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl C\. alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCi_4alkyl, perfluoroCi_4alkoxy, oxo, Ci_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Ci-6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Cχ-6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Cι_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci-6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cø-6alkyl)2 aminocarbonyloxy.
The present invention is also concerned with a method of activating the function of the androgen receptor in a patient, and, in particular, a method wherein the function of the androgen receptor is activated (agonized) in bone and/or muscle tissue and blocked in the prostate of a male patient or in the uterus of a female patient with a compound of structural formula I. The compounds of formula I are useful in the prevention and/or treatment of diseases or conditions caused by androgen deficiency or which can be ameliorated by androgen replacement. These diseases or conditions include osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, postmenopausal symptoms in women, atherosclerosis, hypercholesterolemia, obesity, hyperlipidemia, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis and joint repair. The compounds of the present invention may be used alone or in combination with other active agents. In particular, the compounds of the present invention are useful for the prevention and/or treatment of male and female osteoporosis.
The present invention is also concerned with novel compounds which are selective androgen receptor modulators, pharmaceutical compositions containing these novel compounds in association with a pharmaceutically acceptable carrier, and methods to treat diseases or conditions caused by androgen deficiency or which can be ameliorated by androgen replacement with the novel compounds of the present invention.
DETAILED DESCRIPTION OF THE INVENTION
The present invention provides a method for modulating a function mediated by the androgen receptor in a tissue selective manner in a patient in need of such modulation, comprising administering to the patient a therapeutically effective amount of a compound of structural formula I:
Figure imgf000013_0001
(0 or a pharmaceutically acceptable salt thereof; wherein
"a" represents a single bond or a double bond; Rl is hydrogen, hydroxymethyl, or Cι_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms; R2 is hydrogen, fluorine, or Ci-4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci-4 alkyl when "a" represents a single bond; R3 is hydrogen or Ci_3 alkyl;
R4 is hydrogen, Ci_4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Ci-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Cι_4 alkoxy, Cl-4 alkoxyCi-4 alkoxy, and Cι_3 alkyloxycarbonyl; (d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Ci-6 alkoxy, (h) Ci-6 alkylcarbonyloxy,
(i) Cι_6 alkylthio, (j) Cι_6 alkylsulfonyl, (k) C3.8 cycloalkyl Cθ-6 alkyl, (1) C3_8 cycloheteroalkyl Cθ-6 alkyl, (m) aryl Cθ-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino,
(p) Cι_3 acylamino,
(q) aryl Ci-3 acylamino, (r) Ci-6 alkylamino,
(s) di(Ci_6 alkyl)amino,
(t) aryl Cø-3 alkylamino,
(u) di(aryl Cø-3 alkyl)amino,
(v) C3-6 cycloalkyl Cθ-2 alkylamino, (w) Ci-8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Cι_8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Ci_8 alkylaminocarbonylamino,
(cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Ci-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3..6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R10; wherein R9 and R O are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3-.6 cycloalkyl Cø-3 alkyl, C3..6 cycloheteroalkyl Cø-3 alkyl, and aryl CQ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and NCι_4 alkyloxycarbonyl; wherein the aryl group in all instances above is selected from the group consisting of (1) phenyl,
(2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl, (6) benzoxazolyl,
(7) benzothiazolyl,
(10) benzodihydrofuranyl,
(11) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl, (16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl, (21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl, (26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, C1 _8 alkyl, C3-.8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Ci_6alkyl, amino Cθ-6alkyl, Cι_6 alkylamino Cθ-6alkyl, (Ci-6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Ci_6 alkylthio, aryl Cθ-6alkylthio, Cχ-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cχ-6 alkoxycarbonyl Cø- βalkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Ci-6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCι_4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, C -β alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci_6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy.
In one embodiment of the present invention, the compounds of structural formula I have the β-stereochemical configuration for the hydroxy group at the 17-position of the steroid nucleus as indicated in structural formula la below:
Figure imgf000016_0001
In a second embodiment of the compounds useful in the methods of the present invention, Rl is hydrogen or methyl and R3 and R4 are both hydrogen. In a class of this embodiment, "a" represents a double bond.
In a third embodiment of the compounds useful in the methods of the present invention, one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen, (b) fluorine, (c) Ci-3 alkyl, wherein alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Cι _4 alkoxy, Cι_3 alkoxy-Cι_3 alkoxy, and Cι_3 alkyloxycarbonyl; and
(d) arylmethyl, wherein aryl is selected from the group consisting of phenyl, naphthyl, pyridyl, furanyl, pyrrolyl, thiazolyl, imidazolyl, benzofuranyl, and 1,3- benzodioxolyl, wherein the aryl group is unsubstituted or substituted with one to two groups independently selected from halogen, Cχ_4 alkyl, Cι_4 alkoxy, cyano, trifluoromethyl, and trifluoromethoxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-.6 spirocyclic ring system optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and Nχ-4 alkyloxycarbonyl; or R5 and R6 taken together can be =CR9R10) wherein R9 and RlO are each independently selected from the group consisting of hydrogen, Ci_6 alkyl, C3.6 cycloalkyl-Co-3 alkyl, C3-6 cycloheteroalkyl-Cθ-3 alkyl, aryl Cθ-1 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to
6-membered ring optionally containing a heteroatom selected from O, S, NH,
NCi-4 alkyl, and NCi-4 alkyloxycarbonyl.
In a class of this third embodiment, one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined above. In a subclass of this class, Rl is hydrogen or methyl and R3 and R4 are both hydrogen. In a subclass of this subclass, "a" represents a double bond. In another subclass of this class, R5 is hydrogen and R6 is arylmethyl as depicted in the structural formula below:
Figure imgf000017_0001
In a second class of this third embodiment, R5 and R6 taken together are =CHaryl wherein aryl is as defined above. In a subclass of this class, Rl is hydrogen or methyl and R and R are both hydrogen. In a subclass of this subclass, "a" represents a double bond. In another subclass of this class, the stereochemistry at the 16-position is as depicted in the structural formula below:
Figure imgf000018_0001
Illustrative but non-limiting examples of compounds useful in the methods of the present invention are the following:
17β-hydroxy-7β-methyl-4-aza-5α-androst-l-en-3-one; 17α-hydroxy-7β-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-4,16α-dimethyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-4, 16β-dimethyl-4-aza-5α-androst-l -en-3-one;
16α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
16β-fluoro- 17α-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3-one; 16α-fluoro-17β-hydroxy-4,17α-dimethyl-4-aza-5α-androst-l-en-3-one;
16β-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
2 -fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3 -one;
2β-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
2,2-difluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; 17β-hydroxy-2α,4-dimethyl-4-aza-5α-androstan-3-one;
17β-hydroxy-16α-(methoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-(ethoxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-16α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16α-(carboxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 16α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16α-allyl-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4, 16a, 17α-trimethyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androstan-3-one;
17β-hydroxy- 17α-ethyl-4-methyl-4-aza-5α-androstan-3-one;
17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 16α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(2-fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 6α-(3 -fluorobenzyl)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one; 17β-hydroxy- 6α-(4-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(3-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6α-(4-chlorobenzyl)-4-mefhyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-benzyl-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6α-(2-naphthyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6β-(3-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 6-(3-fluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(3 -trifluoromethylbenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 - one; 17β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(3-methoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one; 17β-hydroxy- 6-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-l-en-3 -one; 17β-hydroxy- 6-(3,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(3,5-dichlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-( 1 -methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one; 17β-hydroxy- 16-cyclohexylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-ethylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-isopropylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-isobutylidene-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 16-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst- 1 -en-3-one;
17β-hydroxy- 16-(l ,3-thiazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3- one; 17β-hydroxy- 16-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(5-hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5α- androst- 1 -en-3 -one ;
17β-hydroxy- 16-(l -methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en- 3-one;
17β-hydroxy- 16-(pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy-16-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3 -one;
17β-hydroxy-16-(3-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 16-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy-16-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy-16-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy-16-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-16-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-(3-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(4-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy-16-(pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy-16-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 16-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy- 16-(2-amino-pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-
3 -one;
17β-hydroxy- 16-(3-carboxymethyl-benzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(3 -carboxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-(3-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; and
17β-hydroxy-16-(benzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; or a pharmaceutically acceptable salt thereof.
The present invention also provides novel compounds of structural formula IT or a pharmaceutically salt thereof which are useful as selective androgen receptor modulators:
Figure imgf000021_0001
(II)
wherein
"a" represents a single bond or a double bond; Rl is hydrogen, hydroxymethyl, or Ci_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or C1.4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci_4 alkyl when "a" represents a single bond; R4 is hydrogen, Cι_4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen,
(b) Ci-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Ci-4 alkoxyCi-4 alkoxy, and Ci-3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano,
(f) hydroxy,
(g) C1 -6 alkoxy,
(h) Cι_6 alkylcarbonyloxy,
(i) Ci-6 alkylthio,
(j) Ci-6 alkylsulfonyl,
(k) C3.8 cycloalkyl Cθ-6 alkyl,
(1) C3_8 cycloheteroalkyl Cθ-6 alkyl,
(m) aryl Cθ-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino,
(p) Ci-3 acylamino,
(q) aryl Ci-3 acylamino,
(r) Cι_6 alkylamino,
(s) di(Cι_6 alkyl)amino,
(t) aryl-Cθ-3 alkylamino,
(u) di(aryl-Cθ-3 alkyl)amino,
(v) C3-6 cycloalkyl Cθ-2 alkylamino,
(w) Cι_8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Ci-8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Cι_8 alkylaminocarbonylamino, (cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Ci-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R10J wherein R9 and RlO are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and NC1 -4 alkyloxycarbonyl; wherein the aryl group in all instances above is selected from the group consisting of (1) phenyl,
(2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl, (6) benzoxazolyl,
(7) benzothiazolyl,
(12) benzodihydrofuranyl,
(13) 1,3-benzodio olyl, (10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl, (16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl, (21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl, (26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Ci_8 alkyl, C3_8 cycloalkyl, C3-.8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Cι_6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci-6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci _6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cι_6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Cι_6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCi_4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci -6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Cι_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Cι_6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Ci-6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy; with the provisos that (a) when "a" is a single or double bond, Rl is hydrogen or methyl, and R2, R5 and R are hydrogen, then R4 is not hydrogen, methyl, allyl, or n-propyl; and (b) when "a" is a single bond, Rl is methyl, and R and R6 are hydrogen, then R5 is not methyl, ethyl, isopropyl, or allyl.
In one embodiment of the novel compounds of the present invention, Rl is hydrogen or methyl and R is hydrogen. In a second embodiment of the novel compounds of the present invention, one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) fluorine, (c) Ci-3 alkyl, wherein alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Cι_4 alkoxy, Cι_3 alkoxy-Cι_3 alkoxy, and C1-.3 alkyloxycarbonyl; and
(d) arylmethyl, wherein aryl is selected from the group consisting of phenyl, naphthyl, pyridyl, furanyl, pyrrolyl, thiazolyl, imidazolyl, benzofuranyl, and 1,3- benzodioxolyl, wherein the aryl group is unsubstituted or substituted with one to two groups independently selected from halogen, Cι_4 alkyl, Cι_4 alkoxy, cyano, trifluoromethyl, and trifluoromethoxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3.6 spirocyclic ring system optionally containing a heteroatom selected from O, S, NH, NCι_4 alkyl, and Ni-4 alkyloxycarbonyl; or R5 and R6 taken together can be =CR9R10; wherein R and RlO are each independently selected from the group consisting of hydrogen, Ci_6 alkyl, C3-6 cycloalkyl-Cθ-3 alkyl, C3-6 cycloheteroalkyl-Cθ-3 alkyl, aryl Cθ-1 alkyl; or R9 and
RlO taken together with the carbon atom to which they are attached can form a 3- to
6-membered ring optionally containing a heteroatom selected from O, S, NH,
NCι_4 alkyl, and NCi-4 alkyloxycarbonyl.
In a class of this second embodiment, one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined above. In a subclass of this class, R5 is hydrogen and R6 is arylmethyl as depicted in the structural formula below:
Figure imgf000025_0001
In another subclass of this class, Rl is hydrogen or methyl and R4 is hydrogen. In a subclass of these two subclasses, "a" represents a double bond.
In a second class of this second embodiment, R5 and R taken together are =CHaryl wherein aryl is as defined above. In a subclass of this class, Rl is hydrogen or methyl and R4 is hydrogen. In a subclass of this subclass, "a" represents a double bond. In another subclass of this class, the stereochemistry at the 16-position is as depicted in the structural formula below:
Figure imgf000026_0001
Illustrative but non-limiting examples of novel compounds of the present invention are the following:
16α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 16β-fluoro- 17α-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
16α-fluoro- 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3-one;
16β-fluoro-l 7β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3-one;
2α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
2β-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3 -one; 2,2-difluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3 -one;
17β-hydroxy-2α,4-dimethyl-4-aza-5α-androstan-3 -one;
17β-hydroxy- 16α-(methoxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-16α-(ethoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16α-(carboxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 16α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
1 β-hydroxy- 16α-allyl-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4, 16a, 17α-trimethyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 17α-ethyl-4-methyl-4-aza-5α-androstan-3 -one;
17β-hydroxy- 16α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16α-(2-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16α-(3 -fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16α-(4-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-(3 -chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-16α-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy 6α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6α-benzyl-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy 6α-(2-naphthyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy 6β-(3-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy 6-(3 -fluorobenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy 6-(3 -methoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy 6-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-1-en -3-one;
17β-hydroxy- 6-(3 ,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3,5-dichlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-( 1 -methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one;
17β-hydroxy- 6-cyclohexylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-ethylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-isopropylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-isobutylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst-1-en- 3-one; 17β-hydroxy-16-(l,3-thiazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 16-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one; 17β-hydroxy-16-(5-hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5α- androst- 1 -en-3-one ;
17β-hydroxy- 16-( 1 -methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-
3-one;
17β-hydroxy- 16-(pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy-16-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy-16-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one; 17β-hydroxy-l 6-(3-trifluoromethoxybenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3- one;
17β-hydroxy- 16-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one;
17β-hydroxy-16-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 16-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-(3-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(4-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16-(pyridin-3 -ylmefhylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-l 6-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l -en-3- one;
17β-hydroxy- 16-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy-16-(2-amino-pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-
3-one; 17β-hydroxy-16-(3-carboxymethyl-benzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy-16-(3-carboxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy-16-(3-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; and 17β-hydroxy- 16-(benzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one; or a pharmaceutically acceptable salt thereof.
The term "alkyl" shall mean straight or branched chain alkanes of one to ten total carbon atoms, or any number within this range (i.e., methyl, ethyl, 1- propyl, 2-propyl, n-butyl, s-butyl, t-butyl, etc.). The term "Co alkyl" (as in "Cθ-8 alkylaryl") shall refer to the absence of an alkyl group.
The term "alkenyl" shall mean straight or branched chain alkenes of two to ten total carbon atoms, or any number within this range.
The term "alkynyl" shall mean straight or branched chain alkynes of two to ten total carbon atoms, or any number within this range.
The term "alkylidene" shall mean a straight or branched chain alkylidene group of one to ten total carbon atoms, or any number within this range.
The term "cycloalkyl" shall mean cyclic rings of alkanes of three to eight total carbon atoms, or any number within this range (i.e., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or cyclooctyl).
The term "cycloheteroalkyl," as used herein, shall mean a 3- to 8- membered fully saturated heterocyclic ring containing one or two heteroatoms chosen from N, O, or S. Examples of cycloheteroalkyl groups include, but are not limited to, piperidinyl, pyrrolidinyl, azetidinyl, morpholinyl, and piperazinyl. In one embodiment of the present invention, cycloheteroalkyl is selected from piperidinyl, pyrrolidinyl, and morpholinyl.
The term "alkoxy," as used herein, refers to straight or branched chain alkoxides of the number of carbon atoms specified (e.g., Ci-5 alkoxy), or any number within this range (i.e., methoxy, ethoxy, etc.). The term "aryl," as used herein, refers to a monocyclic or bicyclic system comprising at least one aromatic ring, wherein the monocylic or bicyclic system contains 0, 1, 2, 3, or 4 heteroatoms chosen from N, O, or S, and wherein the monocylic or bicylic system is either unsubstituted or substituted with one or more groups independently selected from halogen, aryl, Ci-8 alkyl, C3-.8 cycloalkyl, C3..8 cycloheteroalkyl, aryl Cι_6alkyl, amino CQ-6alkyl, Cι_6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Co-6alkyl, Ci-6 alkylthio, aryl Cθ-6alkylthio, Cι_6 alkylsulfinyl, aryl )- 6alkylsulfinyl, Cι_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Cι_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cι_6 alkoxycarbonyl Cθ- 6 lkyl? aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Cι_6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCi-4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6aιkylcarbonyloxy, Cχ-6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Cι_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci-6alkylaminocarbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cl-6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy. Preferably, the aryl group is unsubstituted, mono-, di-, or tri- substituted with one to three of the above- named substituents; more preferably, the aryl group is unsubstituted, mono- or di- substituted with one to two of the above-named substituents.
Whenever the term "alkyl" or "aryl" or either of their prefix roots appears in a name of a substituent (e.g., aryl Cθ-8 alkyl), it shall be interpreted as including those limitations given above for "alkyl" and "aryl." Designated numbers of carbon atoms (e.g., Cθ-8) shall refer independently to the number of carbon atoms in an alkyl or cyclic alkyl moiety or to the alkyl portion of a larger substituent in which alkyl appears as its prefix root.
The terms "arylalkyl" and "alkylaryl" include an alkyl portion where alkyl is as defined above and include an aryl portion where aryl is as defined above. Examples of arylalkyl include, but are not limited to, benzyl, fluorobenzyl, chlorobenzyl, phenylethyl, phenylpropyl, fluorophenylethyl, chlorophenylethyl, thienylmethyl, thienylethyl, and thienylpropyl. Examples of alkylaryl include, but are not limited to, toluene, ethylbenzene, propylbenzene, methylpyridine, ethylpyridine, propylpyridine and butylpyridine.
The term "halogen" shall include iodine, bromine, chlorine, and fluorine.
The term "oxy" means an oxygen (O) atom. The term "thio" means a sulfur (S) atom. The term "oxo" means "=O". The term "carbonyl" means "C=O."
The term "substituted" shall be deemed to include multiple degrees of substitution by a named substitutent. Where multiple substituent moieties are disclosed or claimed, the substituted compound can be independently substituted by one or more of the disclosed or claimed substituent moieties, singly or plurally. By independently substituted, it is meant that the (two or more) substituents can be the same or different. When any variable (e.g., R3, R4, etc.) occurs more than one time in any substituent or in formula I, its definition in each occurrence is independent of its definition at every other occurrence. Also, combinations of substituents and/or variables are permissible only if such combinations result in stable compounds.
Under standard nonmenclature used throughout this disclosure, the terminal portion of the designated side chain is described first, followed by the adjacent functionality toward the point of attachment. For example, a Ci-5 alkylcarbonylamino Cl_6 alkyl substituent is equivalent to
O
-C1-6 alkyl-HN^C1-5 alkyl #
In choosing compounds of the present invention, one of ordinary skill in the art will recognize that the various substituents, i.e. Rl, R , R , etc., are to be chosen in conformity with well-known principles of chemical structure connectivity.
Compounds of the present invention have been found to be tissue- selective modulators of the androgen receptor (SARMs). In one aspect, compounds of the present invention may be useful to activate the function of the androgen receptor in a patient, and in particular to activate the function of the androgen receptor in bone and/or muscle tissue and block or inhibit ("antagonize") the function of the androgen receptor in the prostate of a male patient or in the uterus of a female patient. The activation of the AR in bone can be assayed through stimulation of bone formation in a rodent model of osteoporosis, and the antagonism of the AR in the prostate can be assayed through observation of minimal effects on prostate growth in castrated rodents and antagonism of prostate growth induced by AR agonists, as detailed in the Examples. A further aspect of the present invention is concerned with compounds of structural formula I that block the function of the androgen receptor in the prostate of a male patient or in the uterus of a female patient induced by AR agonists, but not in hair-growing skin or vocal cords, and activate the function of the androgen receptor in bone and or muscle tissue, but not in organs which control blood lipid levels (e.g. liver). The compounds of the present invention may be used to treat and/or prevent conditions which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, inflammatory arthritis and joint repair, HTV-wasting, cancer cachexia, muscular dystrophies, premature ovarian failure, and autoimmune disaease, alone or in combination with other active agents. Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a patient in need of such treatment.
In one embodiment, the compounds of the present invention may be used to treat and/or prevent conditions in a male subject which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to, osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, and muscular dystrophies, alone or in combination with other active agents. Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a male patient in need of such treatment.
In another embodiment, the compounds of the present invention may be used to treat and/or prevent conditions in a female subject which are caused by androgen deficiency or which can be ameliorated by androgen replacement, including, but not limited to, osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, muscular dystrophies, premature ovarian failure, and autoimmune disease, alone or in combination with other active agents. Treatment is effected by administration of a therapeutically effective amount of the compound of structural formula I to a female patient in need of such treatment. The compounds of structural formula I may also be employed as adjuncts to traditional androgen depletion therapy in the treatment of prostate cancer to restore bone, minimize bone loss, and maintain bone mineral density. In this manner, they may be employed together with traditional androgen deprivation therapy, including GnRH agonists/antagonists, such as those disclosed in P. Limonta, et al., "LHRH analogues as anticancer agents: pituitary and extrapituitary sites of action," Exp. Opin. Invest. Drugs, 10: 709-720 (2001); H.J. Strieker, "Luteinizing hormone-releasing hormone antagonists," Urology, 58 (Suppl. 2A): 24-27 (2001); R.P. Millar, et al., "Progress towards the development of non-peptide orally-active GnRH antagonists," British Medical Bulletin, 56: 761-772 (2000); and A.V. Schally et al., "Rational use of agonists and antagonists of LH-RH in the treatment of ' hormone-sensitive neoplasms and gynecologic conditions," Advanced Drug Delivery Reviews, 28: 157-169 (1997). It is also possible that the compounds of structural formula I may be used in combination with antiandrogens, such as flutamide, 2- hydroxyflutamide (the active metabolite of flutamide), nilutamide, and bicalutamide (Casodex™) in the treatment of prostate cancer.
Further, the compounds of the present invention may also be employed in the treatment of pancreatic cancer, either for their androgen antagonist properties or as an adjunct to an antiandrogen, such as flutamide, 2-hydroxyflutamide (the active metabolite of flutamide), nilutamide, and bicalutamide (Casodex™). Compounds of structural formula I have minimal negative effects on lipid metabolism. Therefore, considering their tissue selective androgen agonistic properties, the compounds of this invention have advantages over existing approaches for hormone replacement therapy in hypogonadic (androgen deficient) men.
Additionally, compounds of the present invention can increase the number of blood cells, such as red blood cells and platelets, and can be used for treatment of hematopoietic disorders, such as aplastic anemia.
Representative compounds of the present invention typically display submicromolar binding affinity for the androgen receptor. Compounds of this invention are therefore useful in treating mammals suffering from disorders related to androgen receptor function. Pharmacologically effective amounts of the compound, including the pharmaceutically effective salts thereof, are administered to the mammal, to treat disorders related to androgen receptor function, or which can be improved by the addition of additional androgen, such as osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis and joint repair.
It is generally preferable to administer compounds of the present invention in their enantiomerically pure form. Racemic mixtures can be separated into their individual enantiomers by any of a number of conventional methods. These include chiral chromatography, derivatization with a chiral auxiliary followed by separation by chromatography or crystallization, and fractional crystallization of diastereomeric salts. As used herein, a compound of the present invention which functions as an "agonist" of the androgen receptor can bind to the androgen receptor and initiate a physiological or a pharmacological response characteristic of that receptor. The term "tissue-selective androgen receptor modulator" refers to an androgen receptor ligand that mimics the action of a natural ligand in some tissues but not in others. A "partial agonist" is an agonist which is unable to induce maximal activation of the receptor population, regardless of the amount of compound applied. A "full agonist" induces full activation of the androgen receptor population at a given concentration. A compound of the present invention which functions as an "antagonist" of the androgen receptor can bind to the androgen receptor and block or inhibit the androgen-associated responses normally induced by a natural androgen receptor ligand.
The term "pharmaceutically acceptable salts" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids. Salts derived from inorganic bases include aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic salts, manganous, potassium, sodium, zinc, and the like. Particularly preferred are the ammonium, calcium, lithium, magnesium, potassium, and sodium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, substituted amines including naturally occurring substituted amines, cyclic amines, and basic ion exchange resins, such as arginine, betaine, caffeine, choline, N,N'-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethyl-morpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, tromethamine, and the like.
When the compound of the present invention is basic, salts may be prepared from pharmaceutically acceptable non-toxic acids, including inorganic and organic acids. Such acids include acetic, benzenesulfonic, benzoic, camphorsulfonic, citric, ethanesulfonic, formic, fumaric, gluconic, glutamic, hydrobromic, hydrochloric, isethionic, lactic, maleic, malic, mandelic, methanesulfonic, malonic, mucic, nitric, pamoic, pantothenic, phosphoric, propionic, succinic, sulfuric, tartaric, p- toluenesulfonic acid, trifluoroacetic acid, and the like. Particularly preferred are citric, fumaric, hydrobromic, hydrochloric, maleic, phosphoric, sulfuric, and tartaric acids.
The term "therapeutically effective amount" means the amount the compound of structural formula I that will elicit the biological or medical response of a tissue, system, animal or human that is being sought by the researcher, veterinarian, medical doctor or other clinician. The term "composition" as used herein is intended to encompass a product comprising the specified ingredients in the specified amounts, as well as any product which results, directly or indirectly, from combination of the specified ingredients in the specified amounts.
By "pharmaceutically acceptable" it is meant that the carrier, diluent or excipient must be compatible with the other ingredients of the formulation and not be deleterious to the recipient thereof.
The terms "administration of a compound" and "administering a compound" should be understood to mean providing a compound of the invention or a prodrug of a compound of the invention to the individual in need of treatment. By the term "modulating a function mediated by the androgen receptor in a tissue selective manner" is meant modulating a function mediated by the androgen receptor selectively (or discriminately) in anabolic (bone and/or muscular) tissue (bone and muscular) in the absence of such modulation at androgenic (reproductive) tissue, such as the prostate, testis, seminal vesicles, ovary, uterus, and other sex accessory tissues. In one embodiment the function of the androgen receptor in anabolic tissue is activated whereas the function of the androgen receptor in androgenic tissue is blocked or suppressed.
The administration of the compound of structural formula I in order to practice the present methods of therapy is carried out by administering an effective amount of the compound of structural formula I to the patient in need of such treatment or prophylaxis. The need for a prophylactic administration according to the methods of the present invention is determined via the use of well-known risk factors. The effective amount of an individual compound is determined, in the final analysis, by the physician in charge of the case, but depends on factors such as the exact disease to be treated, the severity of the disease and other diseases or conditions from which the patient suffers, the chosen route of administration, other drugs and treatments which the patient may concomitantly require, and other factors in the physician's judgment.
Generally, the daily dosage of the compound of structural formula I may be varied over a wide range from 0.01 to 1000 mg per adult human per day. Most preferably, dosages range from 0.1 to 200 mg/day. For oral administration, the compositions are preferably provided in the form of tablets containing 0.01 to 1000 mg, particularly 0.01, 0.05, 0.1, 0.5, 1.0, 2.5, 3.0, 5.0, 6.0, 10.0, 15.0, 25.0, 50.0, 75, 100, 125, 150, 175, 180, 200, 225, and 500 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.
The dose may be administered in a single daily dose or the total daily dosage may be administered in divided doses of two, three or four times daily. Furthermore, based on the properties of the individual compound selected for administration, the dose may be administered less frequently, e.g., weekly, twice weekly, monthly, etc. The unit dosage will, of course, be correspondingly larger for the less frequent administration.
When administered via intranasal routes, transdermal routes, by rectal or vaginal suppositories, or through an intravenous solution, the dosage administration will, of course, be continuous rather than intermittent throughout the dosage regimen. Exemplifying the invention is a pharmaceutical composition comprising any of the compounds described above and a pharmaceutically acceptable carrier. Also exemplifying the invention is a pharmaceutical composition made by combining any of the compounds described above and a pharmaceutically acceptable carrier. An illustration of the invention is a process for making a pharmaceutical composition comprising combining any of the compounds described above and a pharmaceutically acceptable carrier.
Formulations of the tissue-selective androgen receptor modulator employed in the present method for medical use comprise the compound of structural formula I together with an acceptable carrier thereof and optionally other therapeutically active ingredients. The carrier must be pharmaceutically acceptable in the sense of being compatible with the other ingredients of the formulation and not being deleterious to the recipient subject of the formulation.
The present invention, therefore, further provides a pharmaceutical formulation comprising the compound of structural formula I together with a pharmaceutically acceptable carrier thereof. The formulations include those suitable for oral, rectal, intravaginal, topical or parenteral (including subcutaneous, intramuscular and intravenous administration). Preferred formulations are those suitable for oral administration.
The formulations may be presented in a unit dosage form and may be prepared by any of the methods known in the art of pharmacy. All methods include the step of bringing the active compound in association with a carrier which constitutes one or more ingredients. In general, the formulations are prepared by uniformly and intimately bringing the active compound in association with a liquid carrier, a waxy solid carrier or a finely divided solid carrier, and then, if needed, shaping the product into the desired dosage form.
Formulations of the present invention suitable for oral administration may be presented as discrete units such as capsules, cachets, tablets or lozenges, each containing a predetermined amount of the active compound; as a powder or granules; or a suspension or solution in an aqueous liquid or non-aqueous liquid, e.g., a syrup, an elixir, or an emulsion.
A tablet may be made by compression or molding, optionally with one or more accessory ingredients. Compressed tablets may be prepared by compressing in a suitable machine the active compound in a free flowing form, e.g., a powder or granules, optionally mixed with accessory ingredients, e.g., binders, lubricants, inert diluents, disintegrating agents or coloring agents. Molded tablets may be made by molding in a suitable machine a mixture of the active compound, preferably in powdered form, with a suitable carrier. Suitable binders include, without limitation, starch, gelatin, natural sugars such as glucose or beta-lactose, corn sweeteners, natural and synthetic gums such as acacia, tragacanth or sodium alginate, carboxymethyl- cellulose, polyethylene glycol, waxes and the like. Lubricants used in these dosage forms include, without limitation, sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like. Disintegrators include, without limitation, starch, methyl cellulose, agar, bentonite, xanthan gum and the like. Oral liquid forms, such as syrups or suspensions in suitably flavored suspending or dispersing agents such as the synthetic and natural gums, for example, tragacanth, acacia, methyl cellulose and the like, may be made by adding the active compound to the solution or suspension. Additional dispersing agents which may be employed include glycerin and the like. Formulations for vaginal or rectal administration may be presented as a suppository with a conventional carrier, i.e., a base that is nontoxic and nonirritating to mucous membranes, compatible with the compound of structural formula I, and is stable in storage and does not bind or interfere with the release of the compound of structural formula I. Suitable bases include: cocoa butter (theobroma oil), polyethylene glycols (such as carbowax and polyglycols), glycol-surfactant combinations, polyoxyl 40 stearate, polyoxyethylene sorbitan fatty acid esters (such as Tween, Myrj, and Arlacel), glycerinated gelatin, and hydrogenated vegetable oils. When glycerinated gelatin suppositories are used, a preservative such as methylparaben or propylparaben may be employed.
Topical preparations containing the active drug component can be admixed with a variety of carrier materials well known in the art, such as, e.g., alcohols, aloe vera gel, allantoin, glycerine, vitamin A and E oils, mineral oil, PPG2 myristyl propionate, and the like, to form, e.g., alcoholic solutions, topical cleansers, cleansing creams, skin gels, skin lotions, and shampoos in cream or gel formulations. The compounds of the present invention can also be administered in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles. Liposomes can be formed from a variety of phospholipids, such as cholesterol, stearylamine or phosphatidylcholines. Compounds of the present invention may also be delivered by the use of monoclonal antibodies as individual carriers to which the compound molecules are coupled. The compounds of the present invention may also be coupled with soluble polymers as targetable drug carriers. Such polymers can include polyvinyl- pyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamide-phenol, polyhydroxy-ethylaspartamidephenol, or polyethylene-oxide polylysine substituted with palmitoyl residues. Furthermore, the compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates and cross- linked or amphipathic block copolymers of hydrogels.
Formulations suitable for parenteral administration include formulations that comprise a sterile aqueous preparation of the active compound which is preferably isotonic with the blood of the recipient. Such formulations suitably comprise a solution or suspension of a compound that is isotonic with the blood of the recipient subject. Such formulations may contain distilled water, 5% dextrose in distilled water or saline and the active compound. Often it is useful to employ a pharmaceutically and pharmacologically acceptable acid addition salt of the active compound that has appropriate solubility for the solvents employed. Useful formulations also comprise concentrated solutions or solids comprising the active compound which on dilution with an appropriate solvent give a solution suitable for parenteral administration.
The compounds of the present invention may be coupled to a class of biodegradable polymers useful in achieving controlled release of a drug, for example, polylactic acid, polyepsilon caprolactone, polyhydroxy butyric acid, polyorthoesters, polyacetals, polydihydropyrans, polycyanoacrylates, and cross-linked or amphipathic block copolymers of hydrogels.
The pharmaceutical composition and method of the present invention may further comprise other therapeutically active compounds usually applied in the treatment and prevention of the above mentioned conditions, including osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, aplastic anemia and other hematopoietic disorders, pancreatic cancer, inflammatory arthritis, and joint repair. For the treatment and prevention of osteoporosis, the compounds of the present invention may be administered in combination with a bone-strengthening agent selected from antiresorptive agents, osteoanabolic agents, and other agents beneficial for the skeleton through mechanisms which are not precisely defined, such as calcium supplements, flavonoids, and vitamin D analogs. The conditions of periodontal disease, bone fracture, and bone damage following bone reconstructive surgery may also benefit from these combined treatments. For example, the compounds of the instant invention may be effectively administered in combination with effective amounts of other agents such as estrogens, bisphosphonates, SERMs, cathepsin K inhibitors, vβ3 integrin receptor antagonists, vacuolar ATPase inhibitors, the polypeptide osteoprotegerin, antagonists of VEGF, thiazolidinediones, calcitonin, protein kinase inhibitors, parathyroid hormone (PTH) and analogs, calcium receptor antagonists, growth hormone secretagogues, growth hormone releasing hormone, insulin-like growth factor, bone morphogenetic protein (BMP), inhibitors of BMP antagonism, prostaglandin derivatives, fibroblast growth factors, vitamin D and derivatives thereof, vitamin K and derivatives thereof, soy isoflavones, calcium salts, and fluoride salts. The conditions of periodontal disease, bone fracture, and bone damage following bone reconstructive surgery may also benefit from these combined treatments. In one embodiment of the present invention, a compound of the instant invention may be effectively administered in combination with an effective amount of a bone-strengthening agent selected from the group consisting of estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative; a bisphosphonate; an antiestrogen or a selective estrogen receptor modulator; an αvβ3 integrin receptor antagonist; a cathepsin K inhibitor; an osteoclast vacuolar ATPase inhibitor; calcitonin; and osteoprotegerin. In the treatment of osteoporosis, the activity of the compounds of the present invention are distinct from that of the anti-resorptive agents: estrogens, bisphosphonates, SERMs, calcitonin, cathepsin K inhibitors, vacuolar ATPase inhibitors, agents interfering with the RANK/RANKL/Osteoprotegerin pathway, p38 inhibitors or any other inhibitors of osteoclast generation or osteoclast activation. Rather than inhibiting bone resorption, the compounds of structural formula I stimulate bone formation, acting preferentially on cortical bone, which is responsible for a significant part of bone strength. The thickening of cortical bone substantially contributes to a reduction in fracture risk, especially fractures of the hip. The combination of the tissue-selective androgen receptor modulators of structural formula I with anti-resorptive agents such as estrogen, bisphosphonates, antiestrogens, SERMs, calcitonin, αvβ3 integrin receptor antagonists, HMG-CoA reductase inhibitors, vacuolar ATPase inhibitors, and cathepsin K inhibitors is particularly useful because of the complementarity of the bone anabolic and antiresorptive actions. Bone antiresportive agents are those agents which are known in the art to inhibit the resorption of bone and include, for example, estrogen and estrogen derivatives which include steroidal compounds having estrogenic activity such as, for example, 17β-estradiol, estrone, conjugated estrogen (PREMARTN®), equine estrogen, 17β-ethynyl estradiol, and the like. The estrogen or estrogen derivative may be employed alone or in combination with a progestin or progestin derivative. Nonlimiting examples of progestin derivatives are norethindrone and medroxy- progesterone acetate.
Bisphosphonates are also bone anti-resorptive agents. Bisphosphonate compounds which may also be employed in combination with a compound of structural formula I of the present invention include: (a) alendronic acid: (4-amino-l-hydroxybutylidene)-bis-phosphonic acid;
(b) alendronate (also known as alendronate sodium or monosodium trihydrate): (4-amino-l-hydroxybutylidene)-bis-phosphonate monosodium trihydrate (alendronic acid and alendronate are described in U.S. Patents 4,922,007, to Kieczykowski et al., issued May 1, 1990, and 5,019,651, to Kieczykowski, issued May 28, 1991, both of which are incorporated by reference herein in their entirety);
(c) [(cycloheptylamino)-methylene]-bis-phosphonate (incadronate), which is described in U.S. Patent 4,970,335, to Isomura et al., issued November 13, 1990, which is incorporated by reference herein in its entirety;
(d) (dichloromethylene)-bis-phosphonic acid (clodronic acid) and the disodium salt (clodronate), which are described in Belgium Patent 672,205 (1966) and J. Org. Chem 32, 4111 (1967), both of which are incorporated by reference herein in their entirety; (e) [l-hydroxy-3-(l-pyrrolidinyl)-propylidene]-bis-phosphonate (EB-1053);
(f) (l-hydroxyethylidene)-bis-phosphonate (etidronate);
(g) [l-hydroxy-3-(methylpentylamino)propylidene]-bis-phosphonate (ibandronate), which is described in U.S. Patent No. 4,927,814, issued May 22, 1990, which is incorporated by reference herein in its entirety; (h) (6-amino-l-hydroxyhexylidene)-bis-phosphonate (neridronate);
(i) [3-(dimethylamino)-l-hydroxypropylidene]-bis-phosphonate (olpadronate);
(j) (3-amino-l-hydroxypropylidene)-bis-phosphonate (pamidronate);
(k) [2-(2-pyridinyl)ethylidene]-bis-phosphonate (piridronate), which is described in U.S. Patent No. 4,761,406, which is incorporated by reference in its entirety; (1) [l-hydroxy-2-(3-pyridinyl)-ethylidene]-bis-phosphonate (risedronate);
(m) {[(4-chlorophenyl)thio]methylene}-bis-phosphonate (tiludronate), which is described in U.S. Patent 4,876,248, to Breliere et al., October 24, 1989, which is incorporated by reference herein in its entirety;
(n) [l-hydroxy-2-(lH-imidazol-l-yl)ethylidene]-bis-phosphonate (zoledronate); and
(o) [l-hydroxy-2-imidazopyridin-(l,2-a)-3-ylethylidene]-bis-phosphonate
(minodronate).
Preferred are bisphosphonates selected from the group consisting of alendronate, clodronate, EB-1053, etidronate, ibandronate, incadronate, minodronate, neridronate, olpadronate, pamidronate, piridronate, risedronate, tiludronate, and zoledronate, and pharmaceutically acceptable salts thereof, and mixtures thereof.
More preferred is alendronate, pharmaceutically acceptable salts thereof, and mixtures thereof. Most preferred is alendronate monosodium trihydrate.
Still further, antiestrogenic compounds such as raloxifene (see, e.g., U.S. Pat. No. 5,393,763), clomiphene, zuclomiphene, enclomiphene, nafoxidene, CI- 680, CI-628, CN-55,945-27, Mer-25, U-11.555A, U-100A, and salts thereof, and the like (see, e.g., U.S. Patent Nos. 4,729,999 and 4,894,373) may be employed in combination with a compound of structural formula I in the methods and compositions of the present invention. These agents are also known as SERMs, or selective estrogen receptor modulators, agents known in the art to prevent bone loss by inhibiting bone resorption via pathways believed to be similar to those of estrogens. These agents may be used in combination with the compounds of the present invention to beneficially treat bone disorders including osteoporosis. Such agents include, for example, tamoxifen, raloxifene, lasofoxifene, toremifene, azorxifene, EM-800, EM-652, TSE 424, clomiphene, droloxifene, idoxifene, and levormeloxifene [Goldstein, et al., "A pharmacological review of selective oestrogen receptor modulators," Human Reproduction Update, 6: 212-224 (2000), and Lufkin, et al., "The role of selective estrogen receptor modulators in the prevention and treatment of osteoporosis," Rheumatic Disease Clinics of North America, 27: 163-185 (2001)]. SERMs are also discussed in "Targeting the Estrogen Receptor with SERMs," Ann. Rep. Med. Chem. 36: 149-158 (2001). αvβ3 Integrin receptor antagonists suppress bone resorption and may be employed in combination with the tissue selective androgen receptor modulators of structural formula I for the treatment of bone disorders including osteoporosis. Peptidyl as well as peptidomimetic antagonists of the αvβ3 integrin receptor have been described both in the scientific and patent literature. For example, reference is made to W.J. Hoekstra and B.L. Poulter, Curr. Med. Chem. 5: 195-204 (1998) and references cited therein; WO 95/32710; WO 95/37655; WO 97/01540; WO 97/37655; WO 98/08840; WO 98/18460; WO 98/18461; WO 98/25892; WO 98/31359; WO 98/30542; WO 99/15506; WO 99/15507; WO 00/03973; EP 853084; EP 854140; EP 854145; US Patent Nos. 5,204,350; 5,217,994; 5,639,754; 5,741,796; 5,780,426; 5,929,120; 5,952,341; 6,017,925; and 6,048,861. Evidence of the ability of αvβ3 integrin receptor antagonists to prevent bone resorption in vitro and in vivo has been presented (see V.W. Engleman et al., "A Peptidomimetic Antagonist of the αvβ3 Integrin Inhibits Bone Resorption In Vitro and Prevents Osteoporosis In Vivo," Clin. Invest. 99: 2284-2292 (1997); S.B. Rodan et al., "A High Affinity Non-Peptide αvβ3 Ligand Inhibits Osteoclast Activity In Vitro and In Vivo," T. Bone Miner. Res. 11: S289 (1996); T.F. Gourvest et al., "Prevention of OVX-Induced Bone Loss With a Non-peptidic Ligand of the αvβ3 Vitronectin Receptor," Bone 23: S612 (1998); M.W. Lark et al., "An Orally Active Vitronectin Receptor αvβ3 Antagonist Prevents Bone Resorption In Vitro and In Vivo in the Ovariectomized Rat," Bone 23: S219 (1998)). Other αvβ3 antagonists are described in R.M. Keenan et al., "Discovery of Potent Nonpeptide Vitronectin Receptor (αvβ3) Antagonists," T. Med. Chem. 40: 2289-2292 (1997); R.M. Keenan et al., "Benzimidazole Derivatives As Arginine Mimetics in 1,4- Benzodiazepine Nonpeptide Vitronectin Receptor (αvβ3) Antagonists," Bioorg. Med. Chem. Lett. 8: 3165-3170 (1998); and R.M. Keenan et al., "Discovery of an Imidazopyridine-Containing 1,4-Benzodiazepine Nonpeptide Vitronectin Receptor (αvβ3) Antagonist With Efficacy in a Restenosis Model," Bioorg. Med. Chem. Lett. 8: 3171-3176 (1998). Still other benzazepine, benzodiazepine and benzocycloheptene αvβ3 integrin receptor antagonists are described in the following patent publications: WO 96/00574, WO 96/00730, WO 96/06087, WO 96/26190, WO 97/24119, WO 97/24122, WO 97/24124, WO 98/14192, WO 98/15278, WO 99/05107, WO 99/06049, WO 99/15170, WO 99/15178, WO 99/15506, and U.S. Patent No. 6,159,964, and WO 97/34865. αvβ3 integrin receptor antagonists having dibenzocycloheptene, dibenzocycloheptane and dibenzoxazepine scaffolds have been described in WO 97/01540, WO 98/30542, WO 99/11626, WO 99/15508, WO 00/33838, U.S. Patent Nos. 6,008,213, and 6,069,158. Other osteoclast integrin receptor antagonists incorporating backbone conformational ring constraints have been described in the patent literature. Published patent applications or issued patents disclosing antagonists having a phenyl constraint include WO 98/00395, WO 99/32457, WO 99/37621, WO 99/44994, WO 99/45927,WO 99/52872, WO 99/52879, WO 99/52896, WO 00/06169, EP 0 820,988, EP 0 820,991, U.S. Patent Nos. 5,741,796; 5,773,644; 5,773,646; 5,843,906; 5,852,210; 5,929,120; 5,952,381; 6,028,223; and 6,040,311. Published patent applications or issued patents disclosing antagonists having a monocyclic ring constraint include WO 99/26945, WO 99/30709, WO 99/30713, WO 99/31099, WO 99/59992, WO 00/00486, WO 00/09503, EP 0796,855, EP 0 928,790, EP 0 928,793, U.S. Patent Nos. 5,710,159; 5,723,480; 5,981,546; 6,017,926; and 6,066,648. Published patent applications or issued patents disclosing antagonists having a bicyclic ring constraint include WO 98/23608, WO 98/35949, WO 99/33798, EP 0 853,084, U.S. Patent Nos. 5,760,028; 5,919,792; and 5,925,655. Reference is also made to the following reviews for additional scientific and patent literature that concern alpha v integrin antagonists: M. E. Duggan, et al., "Ligands to the integrin receptor αvβ3, Exp. Opin. Ther. Patents, 10: 1367-1383 (2000); M. Gowen, et al., "Emerging therapies for osteoporosis," Emerging Drugs, 5: 1-43 (2000); T.S. Kerr, et al., "Small molecule αv integrin antagonists: novel anticancer agents," Exp. Opin. Invest. Drugs, 9: 1271-1291 (2000); and W.H. Miller, et al., "Identification and in vivo efficacy of small-molecule antagonists of integrin αvβ3 (the vitronectin receptor)," Drug Discovery Today, 5: 397-408 (2000).
Cathepsin K, formerly known as cathepsin O2, is a cysteine protease and is described in PCT International Application Publication No. WO 96/13523, published May 9, 1996; U.S. Patent No. 5,501,969, issued March 3, 1996; and U.S. Patent No. 5,736,357, issued April 7, 1998, all of which are incorporated by reference herein in their entirety. Cysteine proteases, specifically cathepsins, are linked to a number of disease conditions, such as tumor metastasis, inflammation, arthritis, and bone remodeling. At acidic pH's, cathepsins can degrade type-I collagen. Cathepsin protease inhibitors can inhibit osteoclastic bone resorption by inhibiting the degradation of collagen fibers and are thus useful in the treatment of bone resorption diseases, such as osteoporosis.
Members of the class of HMG-CoA reductase inhibitors, known as the "statins," have been found to trigger the growth of new bone, replacing bone mass lost as a result of osteoporosis (see The Wall Street Tournal, Friday, December 3, 1999, page B 1). Therefore, the statins hold promise for the treatment of bone resorption. Examples of HMG-CoA reductase inhibitors include statins in their lactonized or dihydroxy open acid forms and pharmaceutically acceptable salts and esters thereof, including but not limited to lovastatin (see US Patent No. 4,342,767); simvastatin (see US Patent No. 4,444,784); dihydroxy open-acid simvastatin, particularly the ammonium or calcium salts thereof; pravastatin, particularly the sodium salt thereof (see US Patent No. 4,346,227); fluvastatin, particularly the sodium salt thereof (see US Patent No. 5,354,772); atorvastatin, particularly the calcium salt thereof (see US Patent No. 5,273,995); cerivastatin, particularly the sodium salt thereof (see US Patent No. 5,177,080), rosuvastatin, also known as ZD-4522 (see US Patent No.5,260,440) and pitavastatin, also referred to as NK-104, itavastatin, or nisvastatin (see PCT international application publication number WO 97/23200).
Osteoclast vacuolar ATPase inhibitors, also called proton pump inhibitors, may also be employed together with the tissue selective androgen receptor modulators of structural formula I. The proton ATPase which is found on the apical membrane of the osteoclast has been reported to play a significant role in the bone resorption process. Therefore, this proton pump represents an attractive target for the design of inhibitors of bone resorption which are potentially useful for the treatment and prevention of osteoporosis and related metabolic diseases [see C. Farina et al., "Selective inhibitors of the osteoclast vacuolar proton ATPase as novel bone antiresorptive agents," DDT, 4: 163-172 (1999)].
The angiogenic factor VEGF has been shown to stimulate the bone- resorbing activity of isolated mature rabbit osteoclasts via binding to its receptors on osteoclasts [see M. Nakagawa et al., "Vascular endothelial growth factor (VEGF) directly enhances osteoclastic bone resorption and survival of mature osteoclasts," FEBS Letters, 473: 161-164 (2000)]. Therefore, the development of antagonists of VEGF binding to osteoclast receptors, such as KDR/Flk-1 and Flt-1, may provide yet a further approach to the treatment or prevention of bone resorption.
Activators of the peroxisome proliferator-activated receptor- γ (PPARγ), such as the thiazolidinediones (TZD's), inhibit osteoclast-like cell formation and bone resorption in vitro. Results reported by R. Okazaki et al. in Endocrinology, 140: 5060-5065 (1999) point to a local mechanism on bone marrow cells as well as a systemic one on glucose metabolism. Nonlimiting examples of PPARγ activators include the glitazones, such as troglitazone, pioglitazone, rosiglitazone, and BRL 49653.
Calcitonin may also be employed together with the tissue selective androgen receptor modulator of structural formula I. Calcitonin is preferentially employed as salmon nasal spray (Azra et al., Calcitonin. 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Silverman, "Calcitonin," Rheumatic Disease Clinics of North America, 27: 187-196, 2001)
Protein kinase inhibitors may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Kinase inhibitors include those disclosed in WO 01/17562 and are in one embodiment selected from inhibitors of P-38. Specific embodiments of P-38 inhibitors useful in the present invention include SB 203580 [Badger et al., "Pharmacological profile of SB 203580, a selective inhibitor of cytokine suppressive binding protein/p38 kinase, in animal models of arthritis, bone resorption, endotoxin shock, and immune function," T. Pharmacol. Exp. Ther.. 279: 1453-1461 (1996)].
Osteoanabolic agents are those agents that are known in the art to build bone by increasing the production of the bone protein matrix. Such osteoanabolic agents include, for example, the various forms of parathyroid hormone (PTH) such as naturally occurring PTH (1-84), PTH (1-34), analogs thereof, native or with substitutions and particularly parathyroid hormone subcutaneous injection. PTH has been found to increase the activity of osteoblasts, the cells that form bone, thereby promoting the synthesis of new bone (Modern Drug Discovery, Vol. 3, No. 8, 2000). In studies reported at the First World Congress on Osteoporosis held in Chicago in Tune 2000, women in combined PTH-estrogen therapy exhibited a 12.8% increase in spinal bone mass and a 4.4% increase in total hip mass. Another study presented at the same meeting showed that PTH could increase bone size as well as density. A clinical trial of the effect of the human parathyroid hormone 1-34 fragment [hPTH(l- 34)] on postmenopausal osteoporotic women resulted in >65% reduction in spine fractures and a 54% reduction in nonvertebral fractures, after a median of 22 months of treatment [see T.M. Hock, Bone, 27: 467-469 (2000) and S. Mohan, et al., Bone, 27: 471-478 (2000), and references cited therein]. Thus, PTH and fragments thereof, such as hPTH(l-34), may prove to be efficacious in the treatment of osteoporosis alone or in combination with other agents, such as the tissue selective androgen receptor modulators of the present invention. An injectable recombinant form of human PTH, Forteo (teriparatide), has received regulatory approval in the U.S. for the treatment of osteoporosis. Also useful in combination with the SARMs of the present invention are calcium receptor antagonists which induce the secretion of PTH as described by Gowen et al., in "Antagonizing the parathyroid calcium receptor stimulates parathyroid hormone secretion and bone formation in osteopenic rats," T. Clin. Invest. 105: 1595-604 (2000). Growth hormone secretagogues, growth hormone, growth hormone releasing hormone and the like are also osteoanabolic agents which may be employed with the compounds according to structural formula I for the treatment of osteoporosis. Representative growth hormone secretagogues are disclosed in U.S. Patent No. 3,239,345; U.S. Patent No. 4,036,979; U.S. Patent No. 4,411,890; U.S. Patent No. 5,206,235; U.S. Patent No. 5,283,241; U.S. Patent No. 5,284,841; U.S. Patent No. 5,310,737; U.S. Patent No. 5,317,017; U.S. Patent No. 5,374,721; U.S. Patent No. 5,430,144; U.S. Patent No. 5,434,261; U.S. Patent No. 5,438,136; U.S. Patent No. 5,494,919; U.S. Patent No. 5,494,920; U.S. Patent No. 5,492,916; U.S. Patent No. 5,536,716; EPO Patent Pub. No. 0,144,230; EPO Patent Pub. No. 0,513,974; PCT Patent Pub. No. WO 94/07486; PCT Patent Pub. No. WO 94/08583; PCT Patent Pub. No. WO 94/11012; PCT Patent Pub. No. WO 94/13696; PCT Patent Pub. No. WO 94/19367; PCT Patent Pub. No. WO 95/03289; PCT Patent Pub. No. WO 95/03290; PCT Patent Pub. No. WO 95/09633; PCT Patent Pub. No. WO 95/11029; PCT Patent Pub. No. WO 95/12598; PCT Patent Pub. No. WO 95/13069; PCT Patent Pub. No. WO 95/14666; PCT Patent Pub. No. WO 95/16675; PCT Patent Pub. No. WO 95/16692; PCT Patent Pub. No. WO 95/17422; PCT Patent Pub. No. WO 95/17423; PCT Patent Pub. No. WO 95/34311; PCT Patent Pub. No. WO 96/02530; Science, 260, 1640-1643 (Tune 11, 1993); Ann. Rep. Med. Chem., 28: 177- 186 (1993); Bioorg. Med. Chem. Lett., 4: 2709-2714 (1994); and Proc. Natl. Acad. Sci. USA, 92: 7001-7005 (1995).
Insulin-like growth factor (IGF) may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Insulin-like growth factors may be selected from Insulin-like Growth Factor I, alone or in combination with IGF binding protein 3 and IGF IT [See Tohannson and Rosen, "The IGFs as potential therapy for metabolic bone diseases," 1996, In: Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Ghiron et al., "Effects of recombinant insulin-like growth factor-I and growth hormone on bone turnover in elderly women," T. Bone Miner. Res. 10: 1844-1852 (1995)].
Bone morphogenetic protein (BMP) may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Bone morphogenetic protein includes BMP 2, 3, 5, 6, 7, as well as related molecules TGF beta and GDF 5 [Rosen et al., "Bone morphogenetic proteins," 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press; and Wang EA, "Bone morphogenetic proteins (BMPs): therapeutic potential in healing bony defects," Trends Biotechnol., 11: 379-383 (1993)].
Inhibitors of BMP antagonism may also be employed together with the tissue selective androgen receptor modulators of structural formula I. BMP antagonist inhibitors are in one embodiment selected from inhibitors of the BMP antagonists SOST, noggin, chordin, gremlin, and dan [Massague and Chen, "Controlling TGF- beta signaling," Genes Dev„ 14: 627-644, 2000; Aspenberg et al., "The bone morphogenetic proteins antagonist Noggin inhibits membranous ossification," I. Bone Miner. Res. 16: 497-500, 2001; Brunkow et al., "Bone dysplasia sclerosteosis results from loss of the SOST gene product, a novel cystine knot-containing protein," Am. I. Hum. Genet. 68: 577-89 (2001)]. The tissue-selective androgen receptor modulators of the present invention may also be combined with the polypeptide osteoprotegerin for the treatment of conditions associated with bone loss, such as osteoporosis. Preferably osteoprotegerin is mammalian osteoprotegerin and more preferably human osteoprotegerin. The polypeptide osteoprotegerin, a member ot the tumor necrosis factor receptor superfamily, is useful to treat bone diseases characterized by increased bone loss, such as osteoporosis. Reference is made to U.S. Patent No. 6,288,032, which is incorporated by reference herein in its entirety.
Prostaglandin derivatives may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Prostaglandin derivatives are in one embodiment selected from agonists of prostaglandin receptor EP1, EP2, EP4, FP and IP or a derivative thereof [Pilbeam et al., "Prostaglandins and bone metabolism," 1996. In: Bilezikian, et al. Ed. Principles of Bone Biology, San Diego: Academic Press; Weinreb et al., "Expression of the prostaglandin E(2) (PGE(2)) receptor subtype EP(4) and its regulation by PGE(2) in osteoblastic cell lines and adult rat bone tissue," Bone, 28: 275-281 (2001)].
Fibroblast growth factors may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Fibroblast growth factors include aFGF, bFGF and related peptides with FGF activity [Hurley Florkiewicz, "Fibroblast growth factor and vascular endothelial growth factor families," 1996. In: T. P. Bilezikian, et al., Ed. Principles of Bone Biology, San Diego: Academic Press].
In addition to bone resorption inhibitors and osteoanabolic agents, there are also other agents known to be beneficial for the skeleton through mechanisms which are not precisely defined. These agents may also be favorably combined with the tissue selective androgen receptor modulators of structural formula I.
Vitamin D and vitamin D derivatives may also be employed together with the tissue selective androgen receptor modulator of structural formula I. Vitamin D and vitamin D derivatives include natural vitamin D, 25-OH- vitamin D3, l ,25(OH)2 vitamin D3, lα-OH- vitamin D3, lα-OH-vitamin D2, dihydrotachysterol, 26,27-F6-lα,25(OH)2 vitamin D3, 19-nor-lα,25(OH)2 vitamin D3, 22-oxacalcitriol, calcipotriol, lα,25(OH)2-16-ene-23-yne-vitamin D3 (Ro 23-7553), EB1089, 20-epi- lα,25(OH)2 vitamin D3, KH1060, ED71, lα,24(S)-(OH)2 vitamin D3, lα,24(R)- (OH)2 vitamin D3 [See, Tones G., "Pharmacological mechanisms of therapeutics: vitamin D and analogs," 1996. In: T. P. Bilezikian, et . al. Ed. Principles of Bone Biology, San Diego: Academic Press].
Vitamin K and vitamin K derivatives may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Vitamin K and vitamin K derivatives include menatetrenone (vitamin K2) [see Shiraki et al., "Vitamin K2 (menatetrenone) effectively prevents fractures and sustains lumbar bone mineral density in osteoporosis," T. Bone Miner. Res., 15: 515-521 (2000)].
Soy isoflavones, including ipriflavone, may be employed together with the tissue selective androgen receptor modulators of structural formula I. Fluoride salts, including sodium fluoride (NaF) and monosodium fluorophosphate (MFP), may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Dietary calcium supplements may also be employed together with the tissue selective androgen receptor modulators of structural formula I. Dietary calcium supplements include calcium carbonate, calcium citrate, and natural calcium salts (Heaney. Calcium. 1996. In: T. P. Bilezikian, et al., Ed., Principles of Bone Biology, San Diego: Academic Press).
Daily dosage ranges for bone resorption inhibitors, osteoanabolic agents and other agents which may be used to benefit the skeleton when used in combination with the compounds of structural formula I are those which are known in the art. In such combinations, generally the daily dosage range for the tissue selective androgen receptor modulator of structural formula I is 0.01 to 1000 mg per adult human per day, more preferably from 0.1 to 200 mg/day. However, adjustments to decrease the dose of each agent may be made due to the increased efficacy of the combined agent. In particular, when a bisphosphonate is employed, dosages of 2.5 to
100 mg/day (measured as the free bisphosphonic acid) are appropriate for treatment, more preferably 5 to 20 mg/day, especially about 10 mg day. Prophylactically, doses of about 2.5 to about 10 mg/day and especially about 5 mg/day should be employed. For reduction in side-effects, it may be desirable to administer the combination of the compound of structural formula I and the bisphosphonate once a week. For once weekly administration, doses of about 15 mg to 700 mg per week of bisphosphonate and 0.07 to 7000 mg of the compound of structural formula I may be employed, either separately, or in a combined dosage form. The compound of structural formula I may be favorably administered in a controlled-release delivery device, particularly for once weekly administration.
For the treatment of atherosclerosis, hypercholesterolemia, and hyperlipidemia, the compounds of structural formula I may be effectively administered in combination with one or more additional active agents. The additional active agent or agents can be lipid-altering compounds such as HMG-CoA reductase inhibitors, or agents having other pharmaceutical activities, or agents that have both lipid-altering effects and other pharmaceutical activities. Examples of HMG-CoA reductase inhibitors include statins in their lactonized or dihydroxy open acid forms and pharmaceutically acceptable salts and esters thereof, including but not limited to lovastatin (see US Patent No. 4,342,767); simvastatin (see US Patent No. 4,444,784); dihydroxy open-acid simvastatin, particularly the ammonium or calcium salts thereof; pravastatin, particularly the sodium salt thereof (see US Patent No. 4,346,227); fluvastatin, particularly the sodium salt thereof (see US Patent No. 5,354,772); atorvastatin, particularly the calcium salt thereof (see US Patent No. 5,273,995); cerivastatin, particularly the sodium salt thereof (see US Patent No. 5,177,080), and nisvastatin, also referred to as NK-104 (see PCT international application publication number WO 97/23200). Additional active agents which may be employed in combination with a compound of structural formula I include, but are not limited to, HMG-CoA synthase inhibitors; squalene epoxidase inhibitors; squalene synthetase inhibitors (also known as squalene synthase inhibitors), acyl- coenzyme A: cholesterol acyltransferase (ACAT) inhibitors including selective inhibitors of ACAT-1 or ACAT-2 as well as dual inhibitors of ACAT-1 and -2; microsomal triglyceride transfer protein (MTP) inhibitors; probucol; niacin; cholesterol absorption inhibitors, such as SCH-58235, also known as ezetimibe and 1- (4-fluorophenyl)-3(R)-[3(S)-(4-fluorophenyl)-3-hydroxypropyl)]-4(S)-(4- hydroxyphenyl)-2-azetidinone, which is described in U.S. Patent Nos. 5,767,115 and 5,846,966; bile acid sequestrants; LDL (low density lipoprotein) receptor inducers; platelet aggregation inhibitors, for example glycoprotein TTb/TTIa fibrinogen receptor antagonists and aspirin; human peroxisome proliferator activated receptor gamma (PPARγ) agonists, including the compounds commonly referred to as glitazones, for example troglitazone, pioglitazone and rosiglitazone and, including those compounds included within the structural class known as thiazolidinediones as well as those PPARγ agonists outside the thiazolidinedione structural class; PPARα agonists, such as clofibrate, fenofibrate including micronized fenofibrate, and gemfibrozil; PPAR dual α/γ agonists; vitamin B6 (also known as pyridoxine) and the pharmaceutically acceptable salts thereof such as the HC1 salt; vitamin B12 (also known as cyanocobalamin); folic acid or a pharmaceutically acceptable salt or ester thereof such as the sodium salt and the methylglucamine salt; anti-oxidant vitamins such as vitamin C and E and beta carotene; beta-blockers; angiotensin IT antagonists such as losartan; angiotensin converting enzyme inhibitors, such as enalapril and captopril; calcium channel blockers, such as nifedipine and diltiazem; endothelin antagonists; agents such as LXR ligands that enhance ABC1 gene expression; bisphosphonate compounds, such as alendronate sodium; and cyclooxygenase-2 inhibitors, such as rofecoxib and celecoxib, as well as other agents known to be useful in the treatment of these conditions.
Daily dosage ranges for HMG-CoA reductase inhibitors when used in combination with the compounds of structural formula I correspond to those which are known in the art. Similarly, daily dosage ranges for the HMG-CoA synthase inhibitors; squalene epoxidase inhibitors; squalene synthetase inhibitors (also known as squalene synthase inhibitors), acyl-coenzyme A: cholesterol acyltransferase
(AC AT) inhibitors including selective inhibitors of ACAT-1 or ACAT-2 as well as dual inhibitors of ACAT-1 and -2; microsomal triglyceride transfer protein (MTP) inhibitors; probucol; niacin; cholesterol absorption inhibitors including ezetimibe; bile acid sequestrants; LDL (low density lipoprotein) receptor inducers; platelet aggregation inhibitors, including glycoprotein JSblJla fibrinogen receptor antagonists and aspirin; human peroxisome proliferator activated receptor gamma (PPARγ) agonists; PPARα agonists; PPAR dual α/γ agonists; vitamin B6; vitamin B12; folic acid; anti-oxidant vitamins; beta-blockers; angiotensin IT antagonists; angiotensin converting enzyme inhibitors; calcium channel blockers; endothelin antagonists; agents such as LXR ligands that enhance ABC1 gene expression; bisphosphonate compounds; and cyclooxygenase-2 inhibitors also correspond to those which are known in the art, although due to the combined action with the compounds of structural formula I, the dosage may be somewhat lower when administered in combination. In accordance with the method of the present invention, the individual components of the combination can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. The instant invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating diseases caused by androgen deficiency or that can be ameliorated by addition of androgen.
Abbreviations Used in the Description of the Preparation of the Compounds of the
Present Invention:
AcOH Acetic acid
BOP Benzotriazol- 1 -yloxytris(dimethylamino)phosphonium hexafluorophosphate
BSTFA Bis(trimethylsilyl)trifluoroacetamide
Bu Butyl calc. Calculated
CH2C12 Methylene chloride
(COCl)2 Oxalyl chloride
CBZ (Cbz) Benzyloxycarbonyl
CuBr2 Copper(TT) bromide
DDQ 2,3-Dichloro-5 ,6-dicyano- 1 ,4-benzoquinone
DEAD Diethyl azodicarboxylate
DTT3AL Diisobutylaluminum hydride
DTLA Diisopropylethylamine
DMAP 4-Dimethylaminopyridine
DMF N,N-Dimethylformamide
DMSO Dimethyl sulfoxide
EDC 1 -(3-Dimethylaminopropyl)3-ethylcarbodiimide HC1
ES-MS Electron-spray mass spectroscopy
Et ethyl
Et2O Diethyl ether
Et3N Triethylamine
EtOAc Ethyl acetate FAB Fast atom bombardment
FN(S O2Ph)2 N-Fluorobenzenesulf onimide
HOBt N-hydroxybenzotriazole
HPLC High-performance liquid chromatography
HRMS High resolution mass spectrum i-PrOH Isopropyl alcohol
LAH Lithium aluminum hydride
LDA Lithium diisopropylamide
Me Methyl
MF molecular formula
MgSO4 Magnesium sulfate
MS mass spectrum
NaBFL Sodium borohydride
Na2SO4 Sodium sulfate
NFSI N-fluorobenzenesulfonimide
Ph Phenyl
PhS(O)Ome Phenyl benzenesulfinate
Pr Propyl r.t. Room temperature
NaHCO3 Sodium hydrogencarbonate
TBSC1 t-Butyldimethylsilyl chloride
TFA Trifluoroacetic acid
THF Tetrahydrofuran
TLC Thin-layer chromatography.
Preparation of the Compounds of the Invention
The compounds of the present invention can be prepared according to the procedures denoted in the following reaction Schemes and Examples or modifications thereof using readily available starting materials, reagents, and conventional procedures or variations thereof well-known to a practitioner of ordinary skill in the art of synthetic organic chemistry. Specific definitions of variables in the Schemes are given for illustrative purposes only and are not intended to limit the procedures described. The selective androgen receptor modulators (SARMs) of structural formula L3 in Scheme 1 are prepared as follows. The starting materials inter alia are 17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one (1-1, Rl = Me) which is disclosed in G.H. Rasmusson et al., I. Med. Chem.. 27: 1690-1701 (1984) and 17β-hydroxy-4- aza-5α-androstan-3-one (1-1, Rl = H) which is disclosed in C.C. Bolt, Rec. Trav. Chim. Pavs-Bas, 57: 905 (1938). Oxidation of the 17-alcohol to afford the 17-ketone 1-2 is effected under Swern oxidative conditions using oxalyl chloride in the presence of triethylamine in DMSO. Addition of a Grignard reagent R4MgBr or an alkyl lithium R4Li across the carbon-oxygen double bond in a suitable solvent such as diethyl ether and tetrahydrofuran affords the 17α-(R4)-substituted-17β-ol 4- azasteroids of formula 1-3.
SCHEME 1
Figure imgf000054_0001
The SARMs of structural formula 2^3 in Scheme 2 are prepared in a similar fashion as those in Scheme 1 but using intermediates of formula 2Λ having a double bond at the C1-C2 position of the 4-azasteroid "A" ring which can be prepared by treating intermediates LI with DDQ and BSTFA in toluene. Representative examples of 2-1 are 17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one (2-1, Rl = Me) and 17β-hydroxy-4-aza-5α-androst-l -en-3 -one (2-1, Rl = H) which are disclosed in G.H. Rasmusson et al., T. Med. Chem., 29: 2298-2315 (1986).
SCHEME 2
Figure imgf000055_0001
The SARMs of structural formula 3_ 2 in Scheme 3 are prepared as follows. Alkylation at the 16-position is effected by treatment of the enolate generated from the 17-ketone of structural formula 3-1 with an organic base such as lithium diisopropylamide (LDA) or an alkali metal hexamethyldisilazide (such as LiHMDS or NaHMDS) in a suitable organic solvent such as diethyl ether and tetrahydrofuran with an electrophile R5βr or R5θTf. The derived 16-alkylated-17- ketone intermediate is then reduced with a suitable reducing agent such as sodium borohydride in a suitable organic solvent to afford stereoselectively the desired 16R5- alkylated 17β-ol compounds of structural formula 3-2. SCHEME 3
Figure imgf000056_0001
"a" = single or double bond
The SARMs of structural formula 4^2 in Scheme 4 can be prepared as follows. A mixed aldol condensation between 17-ketone 3Λ and an aldehyde or ketone of formula 4-1 affords an α,β-unsaturated 17-ketone intermediate which is subjected to 1,2-carbonyl reduction in a stereoselective fashion with a suitable reducing reagent such as sodium borohydride in a suitable organic solvent.
SCHEME 4
Figure imgf000056_0002
"a" = single or double bond
The following examples are provided to further illustrate details for the preparation and use of the compounds of the present invention. They are not intended to be limitations on the scope of the instant invention in any way, and they should not be so construed. Furthermore, the compounds described in the following examples are not to be construed as forming the only genus that is considered as the invention, and any combination of the compounds or their moieties may itself form a genus. Those skilled in the art will readily understand that known variations of the conditions and processes of the following preparative procedures can be used to prepare these compounds. All temperatures are in degrees Celsius unless noted otherwise.
SCHEME 5
Figure imgf000057_0001
EXAMPLE 1
Step A: 4-Methyl-4-aza-5α-androstan- 17-one (5-2)
To a stirred soln of oxalyl chloride (1.60 mL, 18.33 mmol) in CH2C12 (25 ml) at -78°C was added DMSO (1.67 mL, 23.57 mmol). After gas evolution ceased, the alcohol 5^1 (4.0 g, 13.1 mmol) in CH2C12 (25 ml) was added in a stream.
After 30 min, triethylamine (9.13 mL, 65.48 mmol) was added dropwise. After 30 min, the cooling bath was removed. After 1 h, the reaction was diluted with EtOAc and washed with water, IN HC1, sat. NaHCO3, brine, dried (MgSO4) and concentrated to give a solid. Flash chromatography (silica gel, 90 g, 0 to 100% EtOAc/hexane over 10 min as eluent) provided a white solid (3.5 g). HRMS (FAB, M + 1) calcd 304.2198, found 304.2270.
Step B: 4, 17α-Dimethyl- 17β-hvdroxy-4-aza-5α-androstan-3-one (5-3) To 5 2 (8.0 g, 26.36 mmol) in THF (130 mL) at -78°C was added
MeMgBr (10.55 mL, 31.64 mmol). The reaction was warmed to room temperature and stirred for 16 h. The reaction was diluted with CH2C12 and washed with IN HC1, saturated aqueous NaHCO3, H2O, brine, dried (MgSO ) and concentrated to a yellow solid. Flash chromatography (silica gel, 110 g, 0 to 100% EtOAc over 12 min as eluent) provided an off-white solid (4.5 g).
HRMS (FAB, M + 1) calcd 320.2511, found 320.2583.
Step C: 4, 17α-Dimeτhyl- 17β-hydroxy-4-aza-5α-androst- 1 -en-3 -one (5-6)
To 5^3 (500 mg, 1.56 mmol) and DDQ (391 mg, 1.72 mmol) in dioxane (8 mL) was added BSTFA (2.66 mL, 10 mmol). The reaction was heated to reflux. After 18 h, the reaction was cooled to room temperature. The resulting mixture was diluted with CH2C12 and washed with sat. aqueous NaHCO3, IN HC1, brine, dried (MgSO ), and concentrated to give a brown oil. The oil was diluted with 10 mL HCl/dioxane and stirred for 20 min and then concentrated to an oil. Flash chromatography (silica gel, 30g, 0 to 100% EtOAc/hexane over 5 min) provided a white solid (120 mg). MS (ES, M+l) calcd 318, found 318. EXAMPLE 2
Step A: 17α-Eτhvnyl-l 7β-hvdroxy-4-methyl-4-aza-5α-androstan-3-one (5-4)
A solution of 5^2 (200 mg, 0.659 mmol) in THF (2 mL) was cooled to 0°C and added dropwise to a solution of lithium acetylene-ethylenediamine complex (303 mg, 3.29 mmol) and THF (2 mL) cooled to 0°C. The resulting solution was allowed to warm to room temperature and after 12 h was quenched with sat. aqueous NH4CI and diluted with CH2C12 and washed with H2O, brine, dried (MgSO ) and concentrated to an orange oil. Flash chromatography (silica gel, 10 g, 0-100% EtOAc/hexane over 10 min) provided an off-white solid (40 mg). HRMS (FAB, M + 1) calcd 330.2355, found 330.2426.
Step B: 17α-Ethyl-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one (5-5)
A solution of 5^4 (500 mg, 1.52 mmol) in EtOAc (lOmL) was stirred with 5% Pd/C under a H2 balloon for 18 h. The reaction was filtered through a celite pad and concentrated to give a white solid (500 mg). MS (ES, M + 1) calcd 333, found 334.
SCHEME 6
Figure imgf000059_0001
Step A: 17β-Hydroxy-4-aza-5α-androst-l -en-3-one (6-2)
To 6Λ (500 mg, 1.64 mmol) and DDQ (409 mg, 1.80 mmol) in dioxane (6 mL) was added BSTFA (2.70 mL, 10.1 mmol). The reaction was heated to reflux. After 18 h, the reaction was cooled to room temperature. The resulting mixture was diluted with CH2C12 and washed with sat. aqueous NaHCO3, IN HCl, brine, dried (MgSO4), and concentrated to give a brown oil. The oil was diluted with 10 mL HCl/dioxane and stirred for 20 min and then concentrated to an oil. Flash chromatography (silica gel, 35 g, 0 to 100% EtOAc/hexane over 5 min) provided a white solid (156 mg). MS (ES, M+l) calcd 304, found 304.
Step B: 4- Aza-5 α-androst- 1 -en-3, 17-dione (6-3)
Oxalyl chloride (0.98 mL, 11.3 mmol) was dissolved in CH2C12 (100 mL) and cooled to -78°C. DMSO (1.67 mL, 23.5 mmol) was added and the reaction was stirred at -78 °C for 5 min. Alcohol 6_ 2 (2.85 g, 9.4 mmol) was dissolved in CH2C12 (50 mL) and added to the reaction dropwise over 20 min. The reaction was stirred at -78 °C for 1 h. Et3N (6.6 mL, 47.0 mmol) was added and the reaction was stirred at -78 °C for 1 h and then at 0 °C for 2 h. The reaction was quenched by the addition of saturated aqueous NaHCO3 (25 mL) and extracted with CH2C12 (3 x 150 mL). The combined organic layers were dried with NaSO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford ketone ^3 (2.80 g). MS (ES, M+l) calcd 302, found 302.
SCHEME 7
Figure imgf000061_0001
EXAMPLE 3
Step A: 16α-(3-Fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3,17-dione
LT l)
To a solution of ketone 3 (0.500 g, 1.66 mmol) in 20:1 THRDMF (10 mL) at 0 °C was added dropwise a solution of lithium diisopropylamide (1.5 M in cyclohexane, 2.21 mL, 3.32 mmol). After one h, the resulting mixture was cooled to -45 °C, and 3-fluorobenzyl bromide (0.394 mL, 3.32 mmol) was slowly added. The resulting mixture was warmed to -20 °C, and after three h was quenched with water (10 mL) and diluted with CH2C1 (50 mL). The organic portion was separated, dried over magnesium sulfate, and evaporated. The resulting residue was purified by flash chromatography on silica gel (20:1 — > 3:2 CH2Cl2/EtOAc as eluent) to give compound 7 (0.120 g) as a foamy white solid. MS (M + l) found 410.5.
Step B: 16α-(3-Fluorobenzyl)-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-
3-one (7-2) To a solution of ketone 1Λ (0.120 g, 0.292 mmol) in THF (2 mL) at -78 °C was added a solution of lithium aluminum hydride (1.0 M in THF, 0.584 mL, 0.584 mmol). After 30 min, the reaction was quenched with water (25 μL), 5 M NaOH (25 μL) and water (25 μL), and diluted with CH2C12 (20 mL). The organic layer was washed with water (20 mL), dried over magnesium sulfate, and concentrated to provide compound 1^2 (0.115 g) as a white foamy solid. HRMS (FAB, M+l) found 412.2633.
Utilizing the method for preparing 7^2 , and by varying the alkylating reagent in step A, the Examples in Table 1 were prepared.
TABLE 1
Figure imgf000062_0001
Figure imgf000062_0002
Figure imgf000063_0001
Figure imgf000064_0002
SCHEME 8
Figure imgf000064_0001
EXAMPLE 23 Step A: 16-(3,5-Difluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3,17- dione (8-1)
To a solution of ketone &3 (0.100 g, 0.332 mmol) in ethanol (1.5 mL) at 0 °C was added, simultaneously dropwise, aqueous potassium hydroxide (40% solution, 0.830 mL) and ethanolic 3,5-difluorobenzaldehyde (79.0 mg in 0.5 mL, 0.553 mmol). After 30 min, the reaction was warmed to room temperature for 30 minutes and poured into water (50 mL). The resulting white solid was collected by filtration, rinsed with water and dried to provide compound 8 (0.119 g). MS (M + l) found 426.2.
Step B: 16-(3,5-Difluorobenzylidene)-17β-hvdroxy-4-methyl-4-aza-5α- androst-l-en-3-one (8-2)
To a solution of ketone 84 (0.119 g, 0.280 mmol) in THF (2 mL) at -78 °C was added a solution of lithium aluminum hydride (1.0 M in THF, 0.560 mL, 0.560 mmol). After 30 min, the reaction was quenched with water (25 μL), 5 M NaOH (25 μL) and water (25 μL), and diluted with CH2C12 (20 mL). The combined organic layers were washed with water (20 mL), dried over magnesium sulfate, and concentrated to provide compound 8^2 (0.114 g) as a white foamy solid. HRMS (FAB, M+l) found 428.2396.
Utilizing the method for preparing 8^, and by varying the aldehyde in Step A, the Examples in Table 2 were prepared.
TABLE 2
Figure imgf000065_0001
Figure imgf000066_0001
Figure imgf000067_0001
Figure imgf000068_0001
Figure imgf000069_0001
Figure imgf000070_0002
SCHEME 9
Figure imgf000070_0001
Figure imgf000071_0001
EXAMPLE 69
Step A: 16α-Fluoro-4-methyl-4-aza-5α-androst- 1 -en-3 , 17-dione (9- 1 ) Ketone 6X3 (0.163 g, 0.54 mmol) was dissolved in THF (3.0 mL) and cooled to -78°C. LDA (0.47 mL of 1.5 M soln in cyclohexane, 0.70 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min. The reaction was then recooled to -78 °C and N- fluorobenzenesulfonimide (0.255 g dissolved in 1.0 mL of THF, 0.81 mmol) was added dropwise and the reaction was stirred at -78 °C for 15 min. The reaction was warmed to 25 °C and stirred for 2 h. The reaction was quenched by the addition of saturated aqueous NH CI (5 mL) and extracted with CH2C12 (3 x 70 mL). The combined organic layers were dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford fluoroketone 94 (0.122 g). MS (ES, M+H) calcd 320, found 320.
Step B: 16α-Fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one (9-2) and 16α-fluoro-17α-hydroxy-4-mefhyl-4-aza-5α-androst-l-en-3-one (9-3) Fluoroketone 94 (0.100 g, 0.31 mmol) was dissolved in CH2C12 (2.0 mL) and cooled to -78°C. DIBAL (0.34 mL of 1M solution in CH2C12, 0.34 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h. Another 0.30 ml of DIBAL was added and reaction was stirred for 2 hours. The reaction was quenched by the addition of MeOH (2.0 mL) at -78 °C. Saturated Rochelles salt (15 mL) was added and the reaction was extracted with CH2C1 (3 x 70 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by HPLC using Chiropak AD 250 x 4.6 mm column, eluting with hexane:EtOH 70:30, then 60:40 to obtain two products: 9^2 (59 mg, MS (ES, M+H) calcd 322, found 322) and 9^3 (17 mg, MS (ES, M+H) calcd 322, found 322).
EXAMPLE 70
Step A: 16β-Fluoro-4-methyl-4-aza-5α-androst- 1 -en-3 , 17-dione (9-4) Fluoroketone 94 (0.115 g, 0.36 mmol) was dissolved in THF (3.0 mL) and cooled to -78°C. LDA (0.31 mL of 1.5 M soln in cyclohexane, 0.47 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min. The reaction was then recooled to -78 °C and AcOH (0.04 mL, 0.72 mmol) was added dropwise and the reaction was stirred at -78 °C for 15 min. The reaction was quenched by the addition of saturated aqueous
NH CI (5 mL) and extracted with CH2C12 (3 x 70 mL). The combined organic layers were dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes) to afford fluoroketone 9 (0.095 g). MS (ES, M+H) calcd 320, found 320.
Step B: 16β-Fluoro-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one (9-5)
Fluoroketone 9 (0.065 g, 0.20 mmol) was dissolved in THF (2.0 mL) and cooled to -78°C. LAH (0.41 mL of 1M solution in THF, 0.41 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h. The reaction was quenched by the addition of MeOH (2.0 mL) at -78 °C. Saturated Rochelles salt (15 mL) was added and the reaction was extracted with CH2C12 (3 x 70 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes) to afford fluoroalcohol ^_ (0.057 g). MS (ES, M+H) calcd 322, found 322. EXAMPLE 71
Step A: 16β-Fluoro-4, 16α-dimethyl- 17β-hydroxy-5α-androst-l -en-3-one (9-6)
Fluoroketone 9 (0.019 g, 0.06 mmol) was dissolved in Et2O and cooled to -78 °C. MeMgBr (0.06 mL of 3 M solution in Et2O, 0.18 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was allowed to warm to 25 °C and stirred for 18 h. The reaction was quenched by the addition of saturated aqueous NH4CI (5 mL) and extracted with CH2C12 (3 x 70 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes) to afford fluoroalcohol 9^6 (6 mg). MS (ES, M+H) calcd 336, found 336.
SCHEME 10
TBSCI imidazole
Figure imgf000073_0001
Figure imgf000073_0002
Figure imgf000073_0003
Figure imgf000074_0001
EXAMPLE 72
Step A: 17β-(tert-Butyldimethylsilyloxy)-4-methyl-4-aza-5α-androstan-3 -one
004}
To a suspension of alcohol 54 (2.0 g, 6.55 mmol) in DMF was added imidazole (0.668 g, 9.82 mmol) followed by TBSC1 (1.08 g, 7.20 mmol). The reaction was stirred for 14 h. The reaction was diluted with EtOAc and then washed with H20, 10% KHSO4, brine, dried (MgSO4) and then concentrated. Chromatography on silica gel (hexanes to EtOAc as eluent) gave 10-1 (2.1 g) as a white solid.
Step B: 2α-Fluoro-17β-(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5α- androstan-3-one (10-2) To a solution of 104 (0.600 g, 1.43 mmol) in THF (5.0 ml) at -78°C was added 1.5 M LDA in THF (1.14 ml, 1.71 mmol) dropwise over 5 min and then stirred for 1 h. A solution of N-fluorobenzenesulfonimide (2.25 g, 7.15 mmol) in 2 ml THF was added dropwise over 5 min. After 30 min, the cooling bath was removed and the reaction was stirred for 14 h. Et2O was added and then washed with H2O, saturated aqueous NaHCO3, brine, dried (MgSO4) and then concentrated.
Chromatography on silica gel (hexanes to 50%EtOAc/hexanes as eluent) gave 10-2 (0.28 g) as a white solid.
Step C: 2α-Fluoro-l 7β-hydroxy-4-methyl-4-aza-5α-androstan-3-one (10-3) To a solution of 104 (1.0 g, 2.38 mmol) in THF (2.0 ml) at -78°C was added 2.0 M LDA (1.43 ml, 2.86 mmol) dropwise over 5 min, stirred 1 h. A solution of N-fluorobenzenesulfonimide (2.25g, 7.15 mmol) in 2 ml THF was added dropwise over 5 min. After 30 min, the cooling bath was removed and the reaction was stirred for 14 h. 1.0 ml HF was added and the mixture stirred for 30 min. Et2O was then added and the mixture was washed with H O, saturated aqueous sodium hydrogencarbonate solution, brine, dried (MgSO ) and then concentrated. Chromatography on silica gel (hexanes to EtOAc as eluent) gave 10-2 (0.5 g) as a white solid.
MS calculated M+H 324.2334, found 324.2335.
EXAMPLE 73
Step A: 2β-Fluoro-17β-(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5α- androstan-3-one (10-4) To a solution of 1 (0.125 g, 0.286 mmol) in THF (2.0 mL) at -78°C was added a solution of 1.5 M LDA in THF (0.230 mL, 0.340 mmol) dropwise over 5 min and then stirred 1 h. A solution of 0.1 mL AcOH in 2 mL THF was then added dropwise over 5 min. After 30 min, the cooling bath was removed and the reaction was stirred for 14 h. Et2O was added and then washed with H2O, saturated aqueous sodium hydrogencarbonate, brine, dried (MgSO4) and then concentrated.
Chromatography on silica gel (hexanes to EtOAc as eluent) gave 10-4 (0.09 g) as a colorless oil.
Step B: 2β-Fluoro-l 7β-hydroxy-4-methyl-4-aza-5α-androstan-3-one (10-5)
To a solution of 10=4 (0.090 g, 0.286 mmol) in CH3CN (1.0 mL) at was added 1.0 mL HF and then stirred for 30 min. Et2O was added and then washed with H2O, saturated aqueous sodium hydrogencarbonate, brine, dried (MgSO ) and then concentrated. Chromatography on silica gel (hexanes to EtOAc as eluent) gave
10-5 (0.055 g) as a white solid.
MS calculated M+H: 324.2334, found 324.2326.
SCHEME 11
Figure imgf000076_0001
EXAMPLE 74
Step A: 2-Fluoro-17β-(tert-butyldimethylsilyloxy)-4-methyl-4-aza-5α-androst- l-en-3-one (ll-l)
To a stirred solution of 10=2 (0.280 g, 0.640 mmol) in THF at -78°C was added 1.5 M LDA-THF (0.85 mL, 1.28 mmol) dropwise over 5 min and then stirred for 1 h. To the solution was added methyl benzenesulfinate (0.200 g, 1.28 mmol) dropwise over 5 min. After 30 min, the cooling bath was removed and the mixture stirred for 1 h. Et2O was added and the mixture was washed with H2O, saturated aqueous sodium hydrogencarbonate, brine, dried (MgSO ) and then concentrated. The residue was dissolved in toluene and then heated to reflux for 2 h. The solution was concentrated. Chromatography on silica gel (hexanes to 50%EtOAc/hexanes as eluent) gave 11-1 (0.180 g) as a colorless oil.
Step B: 2-Fluoro-l 7 β-hydroxy-4-methyl-4-aza-5 α-androst- 1 -en-3 -one (11-2)
To a stirred solution of 114 (0.180 g, 0.411 mmol) in CH3CN (1.0 ml) was added 1 mL HF, and then the reaction was stirred for 30 min. Et2O was added and the mixture was washed with H2O, saturated aqueous sodium hydrogencarbonate solution, brine, dried (MgSO4) and then concentrated. Chromatography on silica gel (hexanes to 50%EtOAc/hexanes as eluent) gave 11-2 (0.090 g) as a white solid. MS calculated M+H 322.2177, found 322.2169.
SCHEME 12
EXAMPLE 75
Step A: 16α-Bromo-4-methyl-4-aza-5α-androst- 1 -en-3 , 17-dione (12-1)
Ketone 6=3 (0.100 g, 0.33 mmol) was dissolved in /-PrOH (2.5 mL) at 25 °C. CuBr2 (0.230 g, 1.03 mmol) was added and the reaction was heated to 83 °C and stirred for 2 h. The reaction was quenched by the addition of saturated aqueous sodium hydrogencarbonate solution (5 mL) and extracted with CH2C12 (3 x 70 mL). The combined organic layers were dried with NaSO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (80-100% EtOAc in hexanes as eluent) to afford keto bromide 12-1 (0.115 g). MS (ES, M+H) calcd 380, found 380.
Step B: 16 β-Hydroxy-4-methyl-4-aza-5 α-androst- 1 -en-3 , 17-dione (12-2)
Ketobromide 124 (0.028 g, 0.07 mmol) was dissolved in THF (1.0 mL) at 25 °C. 40% aq KOH (1.0 mL) was added and the reaction was stirred 18 h at room temperature. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH2C12 (3 x 50 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (100% EtOAc as eluent) to afford hydroxyketone 12-2 (0.022 g). MS (ES, M+H) calcd 318, found 318.
Step C: 16 β , 17 β-Dihvdroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one (12-3)
Hydroxyketone 12-2 (0.016 g, 0.05 mmol) was dissolved in
MeOH/CH2Cl2 3:1 (2 mL) and cooled to 0 °C. NaBH (0.004 g, 0.10 mmol) was added and the reaction was stirred for 1 h. The reaction was warmed to room temperature and stirred for an additional h. The reaction was quenched by the addition of saturated aqueous NTT CI (2 mL) and extracted with CH2C12 (3 x 50 mL).
The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford diol 12-3 (0.013 g).
MS (ES, M+H) calcd 320, found 320.
EXAMPLE 76
Step A: 16β-Methoxy-4-methyl-4-aza-5α-androst- 1 -en-3 , 17-dione (12-4) Hydroxyketone 12=2 (0.020 g, 0.06 mmol) was dissolved in DMF (1.0 ml) and cooled to 0 °C. NaH (0.002 g, 0.09 mmol) was added and the reaction was stirred at 0 °C for 5 min. Iodomethane (0.01 mL, 0.16 mmol) was added and the reaction was warmed to room temperature and stirred for 3 h. The reaction was quenched by the addition of saturated aqueous NH4CI (2 mL) and extracted with CH2C12 (3 x 50 mL). The combined organic layers were dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes as eluent) to afford methoxyketone 12-4 (0.018 g). MS (ES, M+H) calcd 332, found 332.
Step B: 17β-Hydroxy-16β-methoxy-4-methyl-4-aza-5α-androst-l-en-3-one
(12-5) Methoxyketone 12-4 (0.010 g, 0.03 mmol) was dissolved in
MeOH CH2Cl2 3:1 (1.5 mL) and cooled to 0 °C. NaBH (0.002 g, 0.06 mmol) was added and the reaction was stirred for 1 h. The reaction was warmed to room temperature and stirred for an additional h. The reaction was quenched by the addition of saturated aqueous NH4CI (2 mL) and extracted with CH2C12 (3 x 50 mL).
The combined organic layers were dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes as eluant) to afford alcohol 12=5 (6 mg).
MS (ES, M+H) calcd 334, found 334.
Figure imgf000080_0001
EXAMPLE 77
Step A: 16β-Morpholino-4-methyl-4-aza-5α-androst-l -en-3 , 17-dione (13-1)
Ketone 24 (0.020 g, 0.05 mmol) was dissolved in MeCN (1 mL). Morpholine (0.01 mL, 0.054 mmol) was added and the reaction was stirred for 36 h. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH2C12 (3 x 50 mL). The combined organic layers were dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography on silica gel (90- 100% EtOAc in hexanes as eluent) to afford ketone 13-1 (4 mg). MS (ES, M+H) calcd 387, found 387.
Step B: 17 β-Methyl- 16 β-morpholino-4-methyl-4-aza-5 α-androst- 1 -en-3 -one
(13-2) Ketone 134 (0.004 g, 0.01 mmol) was dissolved in MeOH/CH2Cl2 3:1
(2 mL) and cooled to 0 °C. NaBHU (0.001 g, 0.02 mmol) was added and the reaction was stirred for 1 h. The reaction was warmed to room temperature and stirred for an additional h. The reaction was quenched by the addition of saturated aqueous NH4CI (2 ml) and extracted with CH2C12 (3 x 50 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford alcohol
13r2 (3 mg).
MS (ES, M+H) calcd 389, found 389).
SCHEME 14
Figure imgf000081_0001
Figure imgf000082_0001
EXAMPLE 78
Step A: 16α-(Ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst-l-en-3,17- dione (14-1)
Ketone 6=3 (0.150 g, 0.50 mmol) was dissolved in THF (5.0 mL) and cooled to -78°C. LDA (0.47 mL of 1.5 M soln in cyclohexane, 0.65 mmol) was added dropwise and the reaction was stirred at -78 °C for 45 min. The reaction was warmed to 0 °C and stirred for 15 min. The reaction was then recooled to -78 °C and ethyl iodoacetate (0.09 mL, 0.75 mmol) was added dropwise and the reaction was stirred at -78 °C for 1 h and then warmed to 0 °C and stirred for 15 min. The reaction was quenched by the addition of saturated aqueous NH4CI (5 mL) and extracted with CH C12 (3 x 70mL). The organic layer was dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography (90-100% EtOAc in hexanes as eluent) to afford ester 14-1 (0.110 g). MS (ES, M+H) calcd 388, found 388.
Step B: 16α-(Ethyloxycarbonylmethyl)-17β-hydroxy-4-methyl-4-aza-5α- androst-1 -en-3 -one (14-2)
Ketone 144 (0.038 g, 0.13 mmol) was dissolved in MeOH CH2Cl2 3:1 (3 mL) and cooled to 0 °C. NaBIL (0.010 g, 0.26 mmol) was added and the reaction was stirred for 1 h. The reaction was warmed to room temperature and stirred for an additional h. The reaction was quenched by the addition of saturated aqueous NH4CI (2 mL) and extracted with CH2C12 (3 x 50 mL). The organic layer was dried with Na2SO , filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford alcohol 14=2 (0.030 g). MS (ES, M+H) calcd 389, found 389.
EXAMPLE 79
Step A: 16α-(Carboxymethyl)-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3- one (14-3)
Ester 14-2 (0.020 g, 0.05 mmol) was dissolved in MeOH (1.0 mL) at 25 °C. 40% aqueous KOH (0.5 mL) was added and the reaction was stirred 18 h at room temperature. The reaction was quenched by the addition of 2% HCl (2 mL) and extracted with CH2C12 (3 x 50 mL). The combined organic layers were dried with Na2SO , filtered and concentrated to afford crude acid 14-3 (0.019 g). MS (ES, M+H) calcd 362, found 362).
Step B: 16α-(Benzylaminocarbonylmethyl)-17β-hydroxy-4-methyl-4-aza-5α- androst-l-en-3-one (14-4)
Acid 14=3 (0.015 g, 0.04 mmol) was dissolved in DMF (0.5 mL) and cooled to 0 °C. Benzylamine (0.01 mL, 0.06 mmol) was added dropwise, then EDC (0.012 g, 0.06 mmol) and HOBT (0.008 g, 0.06 mmol). The reaction was stirred at 0 °C for 45 min and then warmed to room temperature and stirred for 18 h. The reaction was quenched by the addition of saturated aqueous NH CI (2 mL) and extracted with CH2C12 (3 x 30 mL). The combined organic layers were dried with Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica gel (90-100% EtOAc in hexanes as eluent) to afford amide 14-4 (0.010 g). MS (ES, M+H) calcd 451, found 451).
EXAMPLE 80 Oral Composition
As a specific embodiment of an oral composition of a compound of this invention, 50 mg of a compound of the present invention is formatted with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size 0 hard gelatin capsule.
EXAMPLE 81 Transdermal Patch Formulation
Ingredient Amount
Compound of formula I 40 g
Silicone fluid 45 g
Colloidal silicone dioxide 2.5 g
The silicone fluid and compound of structural formula I are mixed together and the colloidal silicone dioxide is added to increase viscosity. The material is then dosed into a subsequently heat sealed polymeric laminate comprised of the following: polyester release liner, skin contact adhesive composed of silicone or acrylic polymers, a control membrane which is a polyolefin (e.g. polyethylene, polyvinyl acetate or polyurethane), and an impermeable backing membrane made of a polyester multilaminate. The resulting laminated sheet is then cut into 10 cm^ patches. For 100 Patches.
EXAMPLE 82
Suppository
Ingredient Amount
Compound of structural formula I 25 g
Polyethylene glycol 1000 1481 g
Polyethylene glycol 4000 494 g
The polyethylene glycol 1000 and polyethylene glycol 4000 are mixed and melted.
The compound of structural formula I is mixed into the molten mixture, poured into molds and allowed to cool. For 1000 suppositories.
EXAMPLE 83
Injectable solution
Ingredient Amount
Compound of structural formula I 5 g
Buffering agents q.s.
Propylene glycol 400 mg
Water for injection 600 mL The compound of structural formula I and buffering agents are dissolved in the propylene glycol at about 50°C. The water for injection is then added with stirring and the resulting solution is filtered, filled into ampules, sealed and sterilized by autoclaving. For 1000 Ampules.
EXAMPLE 84
Injectable solution
Ingredient Amount
Compound of structural formula I 5 g
Buffering agents q.s.
Magnesium sulfate heptahydrate 100 mg
Water for injection 880 mL
The compound of structural formula I, magnesium sulfate heptahydrate and buffering agents are dissolved in the water for injection with stirring, and the resulting solution is filtered, filled into ampoules, sealed and sterilized by autoclaving. For 1000 Ampoules.
The following assays were used to characterize the activity of the tissue selective androgen receptor modulators of the present invention.
IN VITRO AND IN VIVO ASSAYS FOR IDENTIFICATION OF COMPOUNDS
WITH SARM ACTIVITY 1. Hydroxylapatite-based Radioligand Displacement Assay of Compound Affinity for Endogenously Expressed AR Materials:
Binding Buffer: TEGM (10 mM Tris-HCl, 1 mM EDTA, 10% glycerol, 1 mM beta- mecaptoethanol, 10 mM Sodium Molybdate, pH 7.2)
50% HAP Slurry: Calbiochem Hydroxylapatite, Fast Flow, in 10 mM Tris, pH 8.0 and l mM EDTA. Wash Buffer: 40 mM Tris, pH7.5, 100 mM KCl, 1 mM EDTA and 1 mM EGTA.
95% EtOH
Methyltrienolone, [17α-methyl-3H], (R1881*); NEN NET590
Methyltrienolone (R1881), NEN NLP005 (dissolve in 95% EtOH)
Dihydrotestosterone (DHT) [1,2,4,5,6,7-3H(N)] NEN NET453 Hydroxylapatite Fast Flow; Calbiochem Cat#391947 Molybdate = Molybdic Acid (Sigma, M1651) MDA-MB-453 cell culture media:
RPMI 1640 (Gibco 11835-055) w/23.8 mM NaHCO3, 2 mM L-glutamine
In 500 mL of complete media Final cone. 10 mL (1M Hepes) 20 mM
5 mL (200 mM L-glu) 4 mM
0.5 mL (10 mg/mL human insulin) 10 μg/mL in 0.01 N HCl Calbiochem#407694-S) 50 mL FBS (Sigma F2442) 10% 1 mL (10 mg/mL Gentamicin 20 μg /mL
Gibco#15710-072)
Cell Passaging:
Cells (Hall R. E., et al, European Tournal of Cancer, 30A: 484-490 (1994)) are rinsed twice in PBS, phenol red-free Trypsin-EDTA is diluted in the same PBS 1:10. The cell layers are rinsed with IX Trypsin, extra Trypsin is poured out, and the cell layers are incubated at 37°C for ~ 2 min. The flask is tapped and checked for signs of cell detachment. Once the cells begin to slide off the flask, the complete media is added to kill the trypsin. The cells are counted at this point, then diluted to the appropriate concentration and split into flasks or dishes for further culturing (Usually 1:3 to 1:6 dilution). Preparation of MDA-MB-453 Cell Lvsate
When the MDA cells are 70 to 85% confluent, they are detached as described above, and collected by centrifuging at 1000 g for 10 min at 4°C. The cell pellet is washed twice with TEGM (10 mM Tris-HCl, 1 mM EDTA, 10% glycerol, 1 mM beta-mercaptoethanol, 10 mM Sodium Molybdate, pH 7.2). After the final wash, the cells are resuspended in TEGM at a concentration of 107 cells/mL. The cell suspension is snap frozen in liquid nitrogen or ethanol/dry ice bath and transferred to -80°C freezer on dry ice. Before setting up the binding assay, the frozen samples are left on ice-water to just thaw (~1 hr). Then the samples are centrifuged at 12,500 g to 20,000 g for 30 min at 4°C. The supernatant is used to set-up assay right away. If using 50 μL of supernatant, the test compound can be prepared in 50 μL of the TEGM buffer. Procedure for Multiple Compound Screening: lx TEGM buffer is prepared, and the isotope-containing assay mixture is prepared in the following order: EtOH (2% final concentration in reaction), 3H- R1881 or 3H-DHT (0.5 nM final Cone, in reaction) and lx TEGM. [eg. For 100 samples, 200 μL (100 x 2) of EtOH + 4.25 μL of 1:10 3H-R1881 stock + 2300 μL (100 x 23) lx TEGM]. The compound is serially diluted, e.g., if starting final cone, is 1 μM, and the compound is in 25 μL of solution, for duplicate samples, 75 μL of 4x1 μM solution is made and 3 μL of 100 μM is added to 72 μL of buffer, and 1:5 serial dilution.
25 μL of 3H-R1881 trace and 25 μL compound solution are first mixed together, followed by addition of 50 μL receptor solution. The reaction is gently mixed, spun briefly at about 200 rpm and incubated at 4°C overnight. 100 μL of 50% HAP slurry is prepared and added to the incubated reaction which is then vortexed and incubated on ice for 5 to 10 minutes. The reaction mixture is vortexed twice more to resuspend HAP while incubating reaction. The samples in 96-well format are then washed in wash buffer using The FilterMate™ Universal Harvester plate washer (Packard). The washing process transfers HAP pellet containing ligand-bound expressed receptor to Unifilter-96 GF/B filter plate (Packard). The HAP pellet on the filter plate is incubated with 50 μL of MTCROSCINT (Packard) scintillint for 30 minutes before being counted on the TopCount microscintillation counter (Packard). IC50S are calculated using R1881 as a reference. Tissue selective androgen receptor modulators of the present invention displayed IC50 values of 1 micromolar or less.
2. MMPl Promoter Suppression, Transient Transfection Assay (TRAMPS) HepG2 cells are cultured in phenol red free MEM containing 10 % charcoal-treated FCS at 37C with 5% CO2. For transfection, cells are plated at 10,000 cells/well in 96 well white, clear bottom plates. Twenty four hours later, cells are co-transfected with a MMPl promoter-lucif erase reporter construct and a rhesus monkey expression construct (50 : 1 ratio) using FuGENEό transfection reagent, following the protocol recommended by manufacturer. The MMPl promoter-lucif erase reporter construct is generated by insertion of a human MMPl promoter fragment (-179/+63) into pGL2 luciferase reporter construct (Promega) and a rhesus monkey AR expression construct is generated in a CMV-Tag2B expression vector (Stratagene). Cells are further cultured for 24 hours and then treated with test compounds in the presence of 100 nM phorbol-12-myristate-13-acetate (PMA), used to increase the basal activity of MMPl promoter. The compounds are added at this point, at a range of lOOOnM to 0.03nM, 10 dilutions, at a concentration on 10X, 1/lOth volume (example: 10 microliters of ligand at 10X added to 100 microliters of media already in the well). Cells are further cultured for an additional 48 hours. Cells are then washed twice with PBS and lysed by adding 70 μL of Lysis Buffer (lx, Promega) to the wells. The luciferase activity is measured in a 96-well format using a 1450 Microbeta let (Perkin Elmer) luminometer. Activity of test compounds is presented as suppression of luciferase signal from the PMA-stimulated control levels. EC50 and Emax values are reported.
Tissue selective androgen receptor modulators of the present invention activate repression typically with submicromolar EC50 values and Emax values greater than about 50%. References: a. Newberry EP, Willis D, Latifi T, Boudreaux TM, Towler DA, "Fibroblast growth factor receptor signaling activates the human interstitial collagenase promoter via the bipartite Ets-APl element," Mol. Endocrinol.11 : 1129-44 (1997).
b. Schneikert T, Peterziel H, Defossez PA, Klocker H, Launoit Y, Cato AC, "Androgen receptor-Ets protein interaction is a novel mechanism for steroid hormone-mediated down-modulation of matrix metalloproteinase expression," J. Biol. Chem. 271: 23907-23913 (1996). 3. A Mammalian Two-Hybrid Assay for the Ligand-induced Interaction of N- Terminus and C-Terminus Domains of the Androgen Receptor (Agonist Mode)
This assay assesses the ability of AR agonists to induce the interaction between the N-terminal domain (NTD) and C-terminal domain (CTD) of rhAR that reflects the in vivo virilizing potential mediated by activated androgen receptors. The interaction of NTD and CTD of rhAR is quantified as ligand induced association between a Gal4DBD-rhARCTD fusion protein and a VP16-rhARNTD fusion protein as a mammalian two-hybrid assay in CV-1 monkey kidney cells.
The day before transfection, CV-1 cells are trypsinized and counted, and then plated at 20,000 cells/well in 96-well plates or larger plates (scaled up accordingly) in DMEM + 10% FCS. The next morning, CV-1 cells are cotransfected with pCBBl (Gal4DBD-rhARLBD fusion construct expressed under the SV40 early promoter), pCBB2 (VP16 -rhAR NTD fusion construct expressed under the SV40 early promoter) and pFR (Gal4 responsive luciferase reporter, Promega) using LTJPOFECTAMINE PLUS reagent (GTBCO-BRL) following the procedure recommended by the vendor. Briefly, DNA admixture of 0.05 μg pCBBl, 0.05 μg pCBB2 and 0.1 μg of pFR is mixed in 3.4 μL OPTI-MEM (GIBCO-BRL) mixed with "PLUS Reagent" (1.6 μL, GIBCO-BRL) and incubated at room temperature (RT) for 15 min to form the pre-complexed DNA.
For each well, 0.4 μL LTPOFECTAMINE Reagent (GTBCO-BRL) is diluted into 4.6 μL OPTI-MEM in a second tube and mixed to form the diluted LTPOFECTAMINE Reagent. The pre-complexed DNA (above) and the diluted LIPOFECTAMTNE Reagent (above) are combined, mixed and incubated for 15 min at RT. The medium on the cells is replaced with 40 μL /well OPTI-MEM, and 10 μL DNA-lipid complexes are added to each well. The complexes are mixed into the medium gently and incubated at 37°C at 5% CO2 for 5h. Following incubation, 200 μL /well D-MEM and 13% charcoal-stripped FCS are added, followed by incubation at 37°C at 5% CO2. After 24 hours, the test compounds are added at the desired concentration(s) (1 nM - 10 μM). Forty eight hours later, luciferase activity is measured using LUC-Screen system (TROPIX) following the manufacturer's protocol. The assay is conducted directly in the wells by sequential addition of 50 μL each of assay solution 1 followed by assay solution 2. After incubation for 40 minutes at room temperature, luminescence is directly measured with 2-5 second integration. Activity of test compounds is calculated as the Emaχ relative to the activity obtained with 3 nM R1881. Typical tissue-selective androgen receptor modulators of the present invention display weak or no agonist activity in this assay with less than 50% agonist activity at 10 micromolar.
Reference:
He B, Kemppainen TA, Voegel IT, Gronemeyer H, Wilson EM, "Activation function in the human androgen receptor ligand binding domain mediates inter-domain communication with the NH(2)-terminal domain," I. Biol. Chem. 274: 37219-37225
(1999).
4. A Mammalian Two-Hybrid Assay For Inhibition of Interaction between N-
Terminus and C-Terminus Domains of Androgen Receptor (Antagonist Mode) This assay assesses the ability of test compounds to antagonize the stimulatory effects of Rl 881 on the interaction between NTD and CTD of rhAR in a mammalian two-hybrid assay in CV-1 cells as described above.
Forty eight hours after transfection, CV-1 cells are treated with test compounds , typically at 10 μM, 3.3 μM, 1 μM, 0.33 μM, 100 nM, 33 nM, 10 nM, 3.3 nM and 1 nM final concentrations. After incubation at 37°C at 5% CO2 for 10 - 30 minutes, an AR agonist methyltrienolone (R1881) is added to a final concentration of 0.3 nM and incubated at 37°C. Forty-eight hours later, luciferase activity is measured using LUC-Screen system (TROPLX) following the protocol recommended by the manufacturer. The ability of test compounds to antagonize the action of R1881 is calculated as the relative luminescence compared to the value with 0.3 nM R1881 alone.
SARM compounds of the present invention typically displayed antagonist activity in the present assay with IC50 values less than 1 micromolar.
5. Trans- Activation Modulation of Androgen Receptor (TAMAR) This assay assesses the ability of test compounds to control transcription from the MMTV-LUC reporter gene in MDA-MB-453 cells, a human breast cancer cell line that naturally expresses the human AR. The assay measures induction of a modified MMTV LTR/promoter linked to the LUC reporter gene.
20,000 to 30,000 cells/well are plated in a white, clear-bottom 96-well plate in "Exponential Growth Medium" which consists of phenol red-free RPMI 1640 containing 10%FBS, 4mM L-glutamine, 20mM HEPES, lOug/mL human insulin, and 20ug/mL gentamicin. Incubator conditions are 37°C and 5% CO2. The transfection is done in batch mode. The cells are trypsinized and counted to the right cell number in the proper amount of fresh media, and then gently mixed with the Fugene/DNA cocktail mix and plated onto the 96-well plate. All the wells receive 200 μl of medium + lipid/DNA complex and are then incubated at 37°C overnight. The transfection cocktail consists of serum-free Optimem, Fugeneό reagent and DNA. The manufacturer's (Roche Biochemical) protocol for cocktail setup is followed. The lipid (μl) to DNA (μg) ratio is approximately 3:2 and the incubation time is 20 min at room temperature. Sixteen to 24 hrs after transfection, the cells are treated with test compounds such that the final DMSO (vehicle) concentration is <3%. The cells are exposed to the test compounds for 48 hrs. After 48 hrs, the cells are lysed by a Promega cell culture lysis buffer for 30-60 min and then the luciferase activity in the extracts is assayed in the 96-well format luminometer. Activity of test compounds is calculated as the Emax relative to the activity obtained with 100 nM R1881.
References: a. R.E. Hall, et al., "MDA-MB-453, an androgen-responsive human breast carcinoma cell line with high androgen receptor expression," Eur. I. Cancer, 30A: 484-490 (1994). b. R.E. Hall, et al., "Regulation of androgen receptor gene expression by steroids and retinoic acid in human breast-cancer cells," Int. I. Cancer., 52: 778-784 (1992).
6. In Vivo Prostate Assay Male Sprague-Dawley rats aged 9-10 weeks, the earliest age of sexual maturity, are used in prevention mode. The goal is to measure the degree to which androgen-like compounds delay the rapid deterioration (—85%) of the ventral prostate gland and seminal vesicles that occurs during a seven day period after removal of the testes (orchiectomy [ORX]). Rats are orchiectomized (ORX). Each rat is weighed, then anesthetized by isoflurane gas that is maintained to effect. A 1.5 cm anteroposterior incision is made in the scrotum. The right testicle is exteriorized. The spermatic artery and vas deferens are ligated with 4.0 silk 0.5cm proximal to the testicle. The testicle is freed by one cut of a small surgical scissors distal to the ligation site. The tissue stump is returned to the scrotum. The same is repeated for the left testicle. When both stumps are returned to the scrotum, the scrotum and overlying skin are sutured closed with 4.0 silk. For Sham-ORX, all procedures excepting ligation and scissors cutting are completed. The rats fully recover consciousness and full mobility within 10-15 minutes. A dose of test compound is administered subcutaneously or orally to the rat immediately after the surgical incision is sutured. Treatment continues for an additional six consecutive days.
Necropsy and Endpoints: The rat is first weighed, then anesthetized in a CO2 chamber until near death. Approximately 5ml whole blood is obtained by cardiac puncture. The rat is then examined for certain signs of death and completeness of ORX. Next, the ventral portion of the prostate gland is located and blunt dissected free in a highly stylized fashion. The ventral prostate is blotted dry for 3-5 seconds and then weighed (VPW). Finally, the seminal vesicle is located and dissected free. The ventral seminal vesicle is blotted dry for 3-5 seconds and then weighed (SVWT).
Primary data for this assay are the weights of the ventral prostate and seminal vesicle. Secondary data include serum LH (luteinizing hormone) and FSH (follicle stimulating hormone), and possible serum markers of bone formation and virilization. Data are analyzed by ANOVA plus Fisher PLSD post-hoc test to identify intergroup differences. The extent to which test compounds inhibit ORX-induced loss of VPW and SVWT is assessed.
7. In Vivo Bone Formation Assay: Female Sprague-Dawley rats aged 7-10 months are used in treatment mode to simulate adult human females. The rats have been ovariectomized (OVX) 75-180 days previously, to cause bone loss and simulate estrogen deficient, osteopenic adult human females. Pre-treatment with a low dose of a powerful anti-resorptive, alendronate (0.0028mpk SC, 2X/wk) is begun on Day 0. On Day 15, treatment with test compound is started. Test compound treatment occurs on Days 15-31 with necropsy on Day 32. The goal is to measure the extent to which androgen-like compounds increase the amount of bone formation, shown by increased fluorochrome labeling, at the periosteal surface.
In a typical assay, nine groups of seven rats each are studied. On Days 19 and 29 (fifth and fifteenth days of treatment), a single subcutaneous injection of calcein (8mg/kg) is given to each rat. Necropsy and Endpoints:
The rat is first weighed, then anesthetized in a CO2 chamber until near death. Approximately 5mL whole blood is obtained by cardiac puncture. The rat is then examined for certain signs of death and completeness of OVX. First, the uterus is located, blunt dissected free in a highly stylized fashion, blotted dry for 3-5 seconds and then weighed (UW). The uterus is placed in 10% neutral-buffered formalin. Next, the right leg is disarticulated at the hip. The femur and tibia are separated at the knee, substantially defleshed, and then placed in 70% ethanol. A 1-cm segment of the central right femur, with the femoral proximal- distal midpoint ats center, is placed in a scintillation vial and dehydrated and defatted in graded alcohols and acetone, then introduced to solutions with increasing concentrations of methyl methacrylate. It is embedded in a mixture of 90% methyl methacrylate :10% dibutyl phthalate, that is allowed to polymerize over a 48-72hr period. The bottle is cracked and the plastic block is trimmed into a shape that conveniently fits the vice-like specimen holder of a Leica 1600 Saw Microtome, with the long axis of the bone prepared for cross-sectioning. Three cross-sections of 85μm thickness are prepared and mounted on glass slides. One section from each rat that approximates the midpoint of the bone is selected and blind-coded. The periosteal surface of each section is assessed for total periosteal surface, single fluorochrome label, double fluorochrome label, and interlabel distance.
Primary data for this assay are the percentage of periosteal surface bearing double label and the mineral apposition rate (interlabel distance(μm)/10d), semi-independent markers of bone formation. Secondary data include uterus weight and histologic features. Tertiary endpoints may include serum markers of bone formation and virilization. Data are analyzed by ANOVA plus Fisher PLSD post-hoc test to identify intergroup differences. The extent to which test compounds increase bone formation endpoint are assessed. While the foregoing specification teaches the principles of the present invention, with examples provided for the purpose of illustration, it is understood that the practice of the invention encompasses all of the usual variations, adoptions, or modifications, as being within the scope of the following claims and their equivalents.

Claims

WHAT IS CLAIMED IS:
1. A method for modulating a function mediated by the androgen receptor in a tissue selective manner in a subject in need thereof comprising administering a therapeutically effective amount of a compound of structural formula I:
Figure imgf000094_0001
(I)
or a pharmaceutically acceptable salt thereof; wherein "a" represents a single bond or a double bond; Rl is hydrogen, hydroxymethyl, or Ci_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or C _4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci-4 alkyl when "a" represents a single bond; R3 is hydrogen or Ci-3 alkyl;
R4 is hydrogen, Cι_4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen, (b) Cι_8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy,
Ci-4 alkoxy, Ci-4 alkoxyCi-4 alkoxy, and Ci-3 alkyloxycarbonyl; (d) fluoro,
(e) cyano,
(f) hydroxy, (g) Ci-6 alkoxy, (h) Ci-6 alkylcarbonyloxy, (i) Ci-6 alkylthio, (j) Ci-6 alkylsulfonyl, (k) C3-8 cycloalkyl Cθ-6 alkyl,
(1) C3-8 cycloheteroalkyl Cθ-6 alkyl, (m) aryl Co-6 alkyl, (n) aryl C2-4 alkenyl,
(o) amino, (p) Ci-3 acylamino,
(q) aryl Cι_3 acylamino,
(r) Ci-6 alkylamino,
(s) di(Cι_6 alkyl)amino,
(t) aryl Cθ-3 alkylamino, (u) di(aryl Cθ-3 alkyl)amino,
(v) C3-6 cycloalkyl Cθ-2 alkylamino,
(w) Cl-8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Cl-8 alkyloxycarbonylamino, (z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Cl-8 alkylaminocarbonylamino, (cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Cl-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R105 wherein R9 and RlO are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3_6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NC1-.4 alkyl, and NC1-.4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of
(1) phenyl,
(2) naphthyl,
(3) benzimidazolyl, (4) benzofuranyl,
(5) benzothiophenyl,
(6) benzoxazolyl,
(7) benzothiazolyl,
(14) benzodihydrofuranyl, (15) 1,3-benzodioxolyl,
(10) indolyl,
(11) quinolyl,
(12) isoquinolyl,
(13) furanyl, (14) thienyl,
(15) imidazolyl,
(16) oxazolyl,
(17) thiazolyl, (18) isoxazolyl, (19) isothiazolyl,
(20) pyrazolyl,
(21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl, (24) pyrazinyl,
(25) thiadiazolyl,
(26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Ci_8 alkyl, C3-8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci_6 alkylamino Co-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Ci-6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci-6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci-6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cι_6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Ci-6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCι_4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci-6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Cι_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Cl -^alkylaminocarbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Ci-6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy.
2. A method of activating the function of the androgen receptor comprising administering to a subject in need thereof a therapeutically effective amount of a compound of structural formula I:
Figure imgf000097_0001
(I)
or a pharmaceutically acceptable salt thereof; wherein "a" represents a single bond or a double bond; Rl is hydrogen, hydroxymethyl, or Ci-3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Ci-4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Cι_4 alkyl when "a" represents a single bond; R is hydrogen or Cι_3 alkyl;
R4 is hydrogen, Ci-4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Cι_8 alkyl, (c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Cι_4 alkoxy, Ci-4 alkoxyCι_4 alkoxy, and Cι_3 alkyloxycarbonyl; (d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Ci-6 alkoxy,
(h) Ci_6 alkylcarbonyloxy, (i) Ci-6 alkylthio,
(j) Ci-6 alkylsulfonyl,
(k) C3-8 cycloalkyl Cθ-6 alkyl,
(1) C3-8 cycloheteroalkyl Cθ-6 alkyl,
(m) aryl Cθ-6 alkyl, (n) aryl C2-4 alkenyl,
(o) amino,
(p) Cι_3 acylamino,
(q) aryl Ci-3 acylamino,
(r) Ci-6 alkylamino, (s) di(Ci_6 alkyl)amino,
(t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino,
(v) C3_6 cycloalkyl Cθ-2 alkylamino,
(w) Cι_8 alkylsulfonylamino, (x) aryl Cθ-3 alkylsulfonylamino,
(y) Ci_8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Cl-8 alkylaminocarbonylamino, (cc) aryl Cθ-3 alkylaminocarbonylamino,
(dd) Ci-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R10, wherein R and RlO are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and NCi-4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of (1) phenyl,
(2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl, (6) benzoxazolyl,
(7) benzothiazolyl,
(16) benzodihydrofuranyl,
(17) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl, (16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl, (21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl, (26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cl-8 alkyl, C3-8 cycloalkyl, C3_8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Ci_6 alkylthio, aryl Cθ-6alkylthio, Ci_6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci-6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Cι_6 alkoxycarbonyl Cθ- βalkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Ci-6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCι_4alkoxy, oxo, Ci_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Cι_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Cι_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci-βalkylamino- carbonylamino, aryl Co-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (C _6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy.
3. The method of Claim 1 wherein said function mediated by the androgen receptor function is activated in bone and/or muscle tissue and blocked in the prostate of a male subject or in the uterus of a female subject.
The method of Claim 3 wherein the compound is of structural formula la
Figure imgf000100_0001
5. The method of Claim 3 wherein Rl is hydrogen or methyl and R3 and R4 are both hydrogen.
6. The method of Claim 5 wherein "a" represents a double bond.
7. The method of Claim 3 wherein one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) fluorine, (c) Ci-3 alkyl, wherein alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Cι_3 alkoxy-Ci-3 alkoxy, and Ci-3 alkyloxycarbonyl; and
(d) arylmethyl, wherein aryl is selected from the group consisting of phenyl, naphthyl, pyridyl, furanyl, pyrrolyl, thiazolyl, imidazolyl, benzofuranyl, and 1,3- benzodioxolyl, wherein the aryl group is unsubstituted or substituted with one to two groups independently selected from halogen, Ci_4 alkyl, Cι_4 alkoxy, cyano, trifluoromethyl, and trifluoromethoxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and Nχ-4 alkyloxycarbonyl; or R5 and R6 taken together can be =CR9R10J wherein R9 and RlO are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3-6 cycloalkyl-Cθ-3 alkyl, C3-6 cycloheteroalkyl-Cθ-3 alkyl, aryl Cθ-1 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, andNCi-4 alkyloxycarbonyl.
8. The method of Claim 7 wherein one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined in Claim 7.
9. The method of Claim 8 wherein Rl is hydrogen or methyl and R3 and R4 are both hydrogen.
10. The method of Claim 9 wherein "a" represents a double bond.
11. The method of Claim 7 wherein R5 and R6 taken together are =CHaryl wherein aryl is as defined in Claim 7.
12. The method of Claim 11 wherein Rl is hydrogen or methyl and
R3 and R are both hydrogen.
13. The method of Claim 12 wherein "a" represents a double bond.
14. The method of Claim 3 wherein the compound is selected from the group consisting of:
17β-hydroxy-7β-methyl-4-aza-5α-androst- 1 -en-3-one;
17α-hydroxy-7β-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4, 16α-dimethyl-4-aza-5α-androst-l -en-3-one; 17β-hydroxy-4, 16β-dimethyl-4-aza-5α-androst- 1 -en-3 -one;
16α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
16β-fluoro- 17α-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
16α-fluoro- 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst-l -en-3-one;
16β-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3-one; 2α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
2β-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3 -one;
2,2-difluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
17β-hydroxy-2α,4-dimethyl-4-aza-5α-androstan-3-one;
17β-hydroxy- 16α-(methoxymethyl)-4-methyl-4-aza-5α-androst-l -en-3-one; 17β-hydroxy- 16α-(ethoxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(carboxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 16α-allyl-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy-4, 16α, 17α-trimethyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androstan-3-one;
17β-hydroxy- 17α-ethyl-4-methyl-4-aza-5α-androstan-3 -one; 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-(3-fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-(4-fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-(3-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-benzyl-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-naphthyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6 β-(3 -methylbenzyl)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one;
17β-hydroxy- 6β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3 -fluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(3 -methoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(3 ,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3,5-dichlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 16-(l-methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy- 6-cyclohexylidene-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-ethylidene-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-isopropylidene-4-methyl-4-aza-5 α-androst- 1 -en-3 -one; 17β-hydroxy- 6-isobutylidene-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst-1-en -3 -one; 17β-hydroxy 6-( 1 ,3 -thiazol-2-ylmethylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one; 17β-hydroxy- 6-(5 -hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5 α- androst-l-en-3 -one; 17β-hydroxy- 6-(l-methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en- 3-one;
17β-hydroxy- 6-(pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy- 6-(3 -trifluoromethoxybenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-andiOSt- 1 -en-3 - one; 17β-hydroxy- 6-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy- 6-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 6-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy- 6-(3-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy-16-(4-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-(pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy-16-(2-amino-pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-
3-one;
17β-hydroxy-16-(3-carboxymethyl-benzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy-16-(3-carboxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-(3-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-l 6-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l -en-3-one; and
17β-hydroxy-16-(benzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; or a pharmaceutically acceptable salt thereof.
15. A method of preventing or treating a condition in a male subject which is caused by androgen deficiency or which can be ameliorated by androgen replacement which condition is selected from the group consisting of osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, and muscular dystrophies, comprising administering to the male subject in need of such prevention or treatment a prophylactically or therapeutically effective amount of a compound of formula I:
Figure imgf000105_0001
(0 or a pharmaceutically acceptable salt thereof; wherein "a" represents a single bond or a double bond; Rl is hydrogen, hydroxymethyl, or Ci-3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Cι_4 alkyl when "a" represents a double bond; or two R substituents are each independently hydrogen, fluorine, or Cι_4 alkyl when "a" represents a single bond;
R3 is hydrogen or Ci-3 alkyl;
R4 is hydrogen, Ci-4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen,
(b) Cl-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Ci_4 alkoxyCι_4 alkoxy, and Cι_3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Ci-6 alkoxy, (h) Ci-6 alkylcarbonyloxy,
(i) Cι_6 alkylthio,
(j) Cι_6 alkylsulfonyl,
(k) C3-8 cycloalkyl Cθ-6 alkyl,
(1) C3_8 cycloheteroalkyl Cθ-6 alkyl, (m) aryl Co-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino, (p) Cχ-3 acylamino, (q) aryl Cι_3 acylamino, (r) Ci-6 alkylamino,
(s) di(Ci_6 alkyl)amino,
(t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino,
(v) C3-6 cycloalkyl CQ-2 alkylamino, (w) Ci_8 alkylsulfonylamino, (x) aryl Cθ-3 alkylsulfonylamino, (y) Cl-8 alkyloxycarbonylamino, (z) aryl Cθ-3 alkyloxycarbonylamino, (aa) aminocarbonylamino,
(bb) Ci_8 alkylaminocarbonylamino, (cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Cι_8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3_6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R10; wherein R9 and RlO are each independently selected from the group consisting of hydrogen, Ci-6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi_4 alkyl, and NCi-4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of (1) phenyl,
(2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl, (6) benzoxazolyl,
(7) benzothiazolyl,
(18) benzodihydrofuranyl,
(19) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl, (16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl, (20) pyrazolyl,
(21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl, (25) thiadiazolyl,
(26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cl-8 alkyl, C3-8 cycloalkyl, C3_8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Ci-6 alkylthio, aryl Cθ-6alkylthio, Ci_6 alkylsulfinyl, aryl Cθ-6a kylsulfinyl, Cι_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Cι_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Ci-6 alkoxycarbonyl )- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Cι_6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCi-4alkyl, perfluoroCι_4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Cι_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Ci_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Cι_6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy.
16. The method of Claim 15 wherein said condition is osteoporosis.
17. The method of Claim 15 wherein the compound is of structural formula III:
Figure imgf000109_0001
or a pharmaceutically acceptable salt thereof; wherein
"a" represents a single bond or a double bond;
Rl is hydrogen, hydroxymethyl, or Cι_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Ci_4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci-4 alkyl when "a" represents a single bond; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Cl-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Ci_4 alkoxyCi-4 alkoxy, and Cι_3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano,
(f) hydroxy, (g) Ci-6 alkoxy,
(h) Ci-6 alkylcarbonyloxy,
(i) Ci-6 alkylthio,
(j) Cι_6 alkylsulfonyl,
(k) C3-8 cycloalkyl Cθ-6 alkyl, (1) C3_8 cycloheteroalkyl Cθ-6 alkyl,
(m) aryl Co-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino, (p) Cι_3 acylamino, (q) aryl C _3 acylamino, (r) Ci-6 alkylamino, (s) di(Ci-6 alkyl)amino, (t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino, (v) C3_6 cycloalkyl Cθ-2 alkylamino, (w) Cι_8 alkylsulfonylamino, (x) aryl Cθ-3 alkylsulfonylamino, (y) Ci-8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Ci-8 alkylaminocarbonylamino, (cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Cι_8 alkylaminocarbonyloxy, and
(ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R105 wherein R and RlO are each independently selected from the group consisting of hydrogen, Cι_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi_4 alkyl, and NCχ_4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of
(1) phenyl,
(2) naphthyl,
(3) benzimidazolyl, (4) benzofuranyl,
(5) benzothiophenyl,
(6) benzoxazolyl,
(7) benzothiazolyl,
(20) benzodihydrofuranyl, (21) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl, (12) isoquinolyl, (13) furanyl,
(14) thienyl,
(15) imidazolyl,
(16) oxazolyl,
(17) thiazolyl, (18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl,
(21) pyrrolyl,
(22) pyridyl, (23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl,
(26) oxadiazolyl,
(27) triazolyl, and (28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cl-8 alkyl, C3-8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Cι_6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci-6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Cι_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Ci_6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Cι_6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCi-4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Ci-6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Ci-6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci_6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Ci-6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl CQ-6alkyl)2 aminocarbonyloxy.
18. The method of Claim 15 wherein the compound is selected from the group consisting of:
17β-hydroxy-7β-methyl-4-aza-5α-androst-l-en-3-one;
17α-hydroxy-7β-methyl-4-aza-5α-androst- 1 -en-3-one; 17β-hydroxy-4, 16α-dimethyl-4-aza-5α-androst-l -en-3-one;
17β-hydroxy-4, 16β-dimethyl-4-aza-5α-androst- 1 -en-3-one;
16α-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one;
16β-fluoro-17α-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one;
16α-fluoro-l 7β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3-one; 16β-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one;
2α-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
2β-fluoro-17β-hydroxy-4-mefhyl-4-aza-5α-androstan-3-one;
2,2-difluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
17β-hydroxy-2α54-dimethyl-4-aza-5α-androstan-3-one; 17β-hydroxy- 16α-(methoxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16α-(ethoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hyαι-oxy-16α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16α-(carboxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy-16α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16α-allyl-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4, 16α, 17α-trimethyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one;
17 β-hydroxy-4, 17α-dimethyl-4-aza-5 α-androstan-3 -one; 17 β-hydroxy- 17α-ethyl-4-methyl-4-aza-5 α-androstan-3 -one;
17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst-l -en-3-one;
17β-hydroxy-16α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy-16α-(2-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(3-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(4-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(3-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16α-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy-16α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-benzyl-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6α-(2-naphthyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6β-(3-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3-fluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-(3-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(3 -methoxybenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one ;
17β-hydroxy- 6-(3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(3,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3,5-dichlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(l-methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-cyclohexylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-ethylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-isopropylidene-4-mefhyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-isobutylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst-1-en- 3 -one; 17β-hydroxy- 6-(l,3-thiazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one; 17β-hydroxy- 6-(5-hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5α- androst-l-en-3 -one;
17β-hydroxy- 6-(l-methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-
3-one;
17β-hydroxy- 6-(pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy- 6-(3 -trifluoromethoxybenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(4-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy- 6-(2-amino-pyridin-3 -ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-
3-one; 17 β-hydroxy- 16-(3 -carboxymethyl-benzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 - one;
17β-hydroxy-16-(3-carboxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-16-(3-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; and
17β-hydroxy- 16-(benzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one; or a pharmaceutically acceptable salt thereof.
19. A method of preventing or treating a condition in a female subject which is caused by androgen deficiency or which can be ameliorated by androgen replacement which condition is selected from the group consisting of postmenopausal osteoporosis, osteopenia, glucocorticoid-induced osteoporosis, periodontal disease, HTV-wasting, cancer cachexia, obesity, aplastic and other anemias, muscular dystrophies, premature ovarian failure, and autoimmune disease, comprising administering to the female subject in need of such prevention or treatment a prophylactically or therapeutically effective amount of a compound of formula I:
Figure imgf000115_0001
(I)
or a pharmaceutically acceptable salt thereof; wherein "a" represents a single bond or a double bond;
Rl is hydrogen, hydroxymethyl, or Cι_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Ci-4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Ci-4 alkyl when "a" represents a single bond;
R3 is hydrogen or Cι_3 alkyl;
R4 is hydrogen, Ci-4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Ci-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Cι_4 alkoxyCi-4 alkoxy, and Ci_3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Cι_6 alkoxy,
(h) Ci_6 alkylcarbonyloxy,
(i) Ci-6 alkylthio,
(j) C 1-6 alkylsulfonyl,
(k) C3_8 cycloalkyl Cθ-6 alkyl,
(1) C3-8 cycloheteroalkyl Cθ-6 alkyl,
(m) aryl Cθ-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino,
(p) Ci-3 acylamino,
(q) aryl Cι_3 acylamino,
(r) Ci-6 alkylamino,
(s) di(Cι_6 alkyl)amino,
(t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino,
(v) C3_6 cycloalkyl Cθ-2 alkylamino,
(w) Cl-8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Cι_8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino,
(bb) Ci-8 alkylaminocarbonylamino,
(cc) aryl Co-3 alkylaminocarbonylamino, (dd) Cι_8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R105 wherein R and RlO are each independently selected from the group consisting of hydrogen, Ci_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and Rl taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and NCi-4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of
(1) phenyl,
(2) naphthyl, (3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl,
(6) benzoxazolyl,
(7) benzothiazolyl, (22) benzodihydrofuranyl,
(23) 1,3-benzodioxolyl,
(10) indolyl,
(11) quinolyl,
(12) isoquinolyl, (13) furanyl,
(14) thienyl,
(15) imidazolyl,
(16) oxazolyl,
(17) thiazolyl, (18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl,
(21) pyrrolyl,
(22) pyridyl, (23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl,
(26) oxadiazolyl, (27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cl-8 alkyl, C3_8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Cι_6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Cι_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Ci-6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Ci-6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCi-4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci-6 alkylsulfonylamino, aryl Co-όalkylsulfonylamino, Ci_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci-βalkylamino- carbonylamino, aryl Co-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl CQ-6alkyl)2 aminocarbonyloxy.
20. The method of Claim 19 wherein said condition is osteoporosis.
21. The method of Claim 19 wherein the compound is of structural formula ITT:
Figure imgf000118_0001
or a pharmaceutically acceptable salt thereof; wherein
"a" represents a single bond or a double bond;
Rl is hydrogen, hydroxymethyl, or Cι_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms; R is hydrogen, fluorine, or Ci_4 alkyl when "a" represents a double bond; or two R substituents are each independently hydrogen, fluorine, or Ci_4 alkyl when "a" represents a single bond; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of (a) hydrogen,
(b) Ci-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, C i -4 alkoxy, C i -4 alkoxyC i -4 alkoxy, and C i _3 alkyloxycarbonyl ;
(d) fluoro,
(e) cyano,
(f) hydroxy,
(g) Ci-6 alkoxy, (h) Cχ-6 alkylcarbonyloxy,
(i) Ci-6 alkylthio,
(j) Cι_6 alkylsulfonyl,
(k) C3-8 cycloalkyl Cθ-6 alkyl,
(1) C3_8 cycloheteroalkyl Cθ-6 alkyl, (m) aryl Cθ-6 alkyl,
(n) aryl C2-4 alkenyl,
(o) amino,
(p) Ci-3 acylamino,
(q) aryl Ci-3 acylamino, (r) Ci-6 alkylamino,
(s) di(Ci_6 alkyl)amino,
(t) aryl Cθ-3 alkylamino,
(u) di(aryl Cθ-3 alkyl)amino,
(v) C3-6 cycloalkyl Cθ-2 alkylamino, (w) Ci-8 alkylsulfonylamino, (x) aryl Cθ-3 alkylsulfonylamino, (y) Cι_8 alkyloxycarbonylamino, (z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Cι_8 alkylaminocarbonylamino,
(cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Cl-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3_6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9R105 wherein R and RlO are each independently selected from the group consisting of hydrogen, Ci_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3_6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH,
NCi-4 alkyl, and NCi-4 alkyloxycarbonyl; wherein the aryl group above is selected from the group consisting of
(1) phenyl, (2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl,
(6) benzoxazolyl, (7) benzothiazolyl,
(24) benzodihydrofuranyl,
(25) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl, (12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl,
(16) oxazolyl, (17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl, (21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl, (26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cι_8 alkyl, C3_8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Cι_6 alkylamino Cθ-6alkyl, (Cι_6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Cι_6 alkylthio, aryl Cθ-6alkylthio, Cι_6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Cι_6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Ci_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Ci-6 alkoxycarbonyl Cθ- 6alkyl, aryl Cθ-6 alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Cι_6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCι_4alkyl, perfluoroCι_4alkoxy, oxo, Cι_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Ci-6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Ci-6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Cι_6alkylamino- carbonylamino, aryl Cθ-6alkylaminocarbonylamino, (Cι_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy.
22. The method of Claim 19 wherein the compound is selected from the group consisting of:
17β-hydroxy-7β-methyl-4-aza-5α-androst-l-en-3-one;
17α-hydroxy-7β-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy-4,l 6α-dimethyl-4-aza-5α-androst-l -en-3-one;
17β-hydroxy-4, 16β-dimethyl-4-aza-5α-androst- 1 -en-3 -one; 16α-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androst-l-en-3-one; 6β-fluoro-l 7α-hydroxy-4-methyl-4-aza-5α-androst-l -en-3-one; 6α-fluoro- 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3 -one; 6 β-fluoro- 17β-hydroxy-4-methyl-4-aza-5 α-androst- 1 -en-3 -one ; α-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; β-fluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; ,2-difluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; 7β-hydroxy-2α,4-dimethyl-4-aza-5α-androstan-3 -one; 7β-hydroxy-16α-(methoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7 β-hydroxy- 16α-(ethoxymethyl)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one; 7β-hydroxy- 16α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 7β-hydroxy-16α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-16α-(carboxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-l 6α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst-l -en-3-one; 7β-hydroxy-16α-allyl-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-4, 16α, 17α-trimethyl-4-aza-5α-androst- 1 -en-3 -one; 7 β-hydroxy-4-methyl-4-aza-5 α-androstan-3 -one ; 7β-hydroxy-4, 17α-dimethyl-4-aza-5α-androstan-3-one; 7β-hydroxy- 17α-ethyl-4-methyl-4-aza-5α-androstan-3-one; 7β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3 -one; 7β-hydroxy-16α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-16α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-16α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-16α-(2-flubrobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7 β-hydroxy- 16α-(3 -fluorobenzyl)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one; 7β-hydroxy-16α-(4-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy- 16α-(3-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one; 7β-hydroxy- 16α-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 7β-hydroxy-16α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-l 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l -en-3-one; 7β-hydroxy- 16α-benzyl-4-methyl-4-aza-5α-androst-l -en-3-one; 7β-hydroxy-16α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-16α-(2-naphthyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 7β-hydroxy-l 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l -en-3-one; 7β-hydroxy- 16β-(3 -methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 7β-hydroxy-16β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3 -fluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17 β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(3 -methoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3 ,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(3,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3 ,5 -dichlorobenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one ;
17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-( 1 -methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one;
17β-hydroxy- 6-cyclohexylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-ethylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-isopropylidene-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-isobutylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(l,3-thiazol-2-ylmethylidene)-4-methyl-4-aza-5α-anάiOst-l-en-3- one;
17β-hydroxy- 6-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(5-hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(l-methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en- 3-one; 17β-hydroxy- 6-(pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(3-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 6-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3 -hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(4-hydroxybenzylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one ;
17β-hydroxy- 6-(pyridin-3 -ylmethylidene)-4-methyl-4-aza-5 α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy- 6-(2-amino-pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-
3 -one;
17β-hydroxy- 6-(3-carboxymethyl-benzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(3 -carboxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(3-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst-l -en-3-one; and
17β-hydroxy- 6-(benzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; or a pharmaceutically acceptable salt thereof.
23. The method of Claim 20 which further comprises the administration of a bone-strengthening agent selected from the group consisting of:
(a) an estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative, (b) a bisphosphonate,
(c) an antiestrogen or a selective estrogen receptor modulator,
(d) an αvβ3 integrin receptor antagonist,
(e) a cathepsin K inhibitor,
(f) an HMG-CoA reductase inhibitor, (g) an osteoclast vacuolar ATPase inhibitor,
(h) an antagonist of VEGF binding to osteoclast receptors,
(i) an activator of peroxisome proliferator-activated receptor γ,
(j) calcitonin,
(k) a calcium receptor antagonist, (1) parathyroid hormone or analog thereof,
(m) a growth hormone secretagogue,
(n) human growth hormone,
(o) insulin-like growth factor,
(p) a P-38 protein kinase inhibitor, (q) bone morphogenetic protein,
(r) an inhibitor of BMP antagonism,
(s) a prostaglandin derivative,
(t) vitamin D or vitamin D derivative,
(u) vitamin K or vitamin K derivative, (v) ipriflavone,
(w) fluoride salts,
(x) dietary calcium supplement, and
(y) osteoprotegerin.
24. The method of Claim 23 wherein:
(a) the estrogen or estrogen derivative, alone or in combination with a progestin or progestin derivative, is selected from conjugated estrogen, equine estrogen, 17β-estradiol, estrone, 17β-ethynyl estradiol, alone or in combination with an agent selected from norethindrone and medroxyprogesterone acetate; (b) the bisphosphonate is selected from: ( 1 ) (4-amino- 1 -hydroxybutylidene)-bisphosphonate ;
(2) [(cycloheptylamino)-methylene]-bisphosphonate;
(3) (dichloromethylene)-bisphosphonate;
(4) [ 1 -hydroxy-3 -( 1 -pyrrolidinyl)-propylidene] -bisphosphonate ; (5) (l-hydroxyethylidene)-bisphosphonate;
(6) [l-hydroxy-3-(methylpentylamino)propylidene]- bisphosphonate;
(7) (6-amino-l-hydroxyhexylidene)-bisphosphonate;
(8) [3-(dimethylamino)-l-hydroxypropylidene]- bisphosphonate;
(9) (3-amino-l-hydroxypropylidene)-bisphosphonate;
(10) [2-(2-pyridinyl)ethylidene]-bisphosphonate;
(11) [l-hydroxy-2-(3-pyridinyl)-ethylidene]-bisphosphonate;
(12) { [(4-chlorophenyl)thio]methylene } -bisphosphonate; (13) [l-hydroxy-2-(lH-imidazol-l-yl)ethylidene]- bisphosphonate; and (14) [1 -hydroxy-2-imidazopyridin-( 1 ,2-a)-3-ylethylidene] - bisphosphonate;
(c) the antiestrogen or selective estrogen receptor modulator is selected from the group consisting of raloxifene, clomiphene, zuclomiphene, enclomiphene, nafoxidene, CI-680, CI-628, CN-55,945-27, Mer-25, U-11,555A, U-100A, tamoxifen, lasofoxifene, toremifene, azorxifene, EM-800, EM-652, TSE 424, droloxifene, idoxifene, and levormeloxifene;
(d) the HMG-CoA reductase inhibitor is selected from lovastatin, simvastatin, dihydroxy-open acid simvastatin, pravastatin, fluvastatin, atorvastatin, cerivastatin, rosuvastatin, pitavastatin, and nisvastatin;
(e) calcitonin is salmon calcitonin administered as a nasal spray;
(f) bone morphogenetic protein is selected from BMP 2, BMP 3, BMP 5, BMP 6, BMP 7, TGF beta, and GDF5; (g) insulin-like growth factor is selected from IGF I and IGF IT alone or in combination with IGF binding protein 3; (h) the prostaglandin derivative is selected from agonists of prostaglandin receptors EP1, EP2, EP4, FP, and IP; (i) the fibroblast growth factor is selected from aFGF and bFGF; (j) parathyroid hormone (PTH) or PTH analog is selected from PTH subcutaneous injection, human PTH (1-84), human PTH (1-34), and other partial sequences, native or with substitutions;
(k) vitamin D or vitamin D derivative is selected from natural vitamin D, 25-OH- vitamin D3, lα,25(OH)2 vitamin D3, lα-OH-vitamin D3, lα-OH-vitamin D2, dihydrotachysterol, 26,27-F6-lα,25(OH)2 vitamin D3, 19-nor-lα,25(OH)2 vitamin D3, 22-oxacalcitriol, calcipotriol, lα,25(OH)2-16-ene-23-yne-vitamin D3 (Ro 23-7553), EB1089, 20-epi-lα,25(OH)2 vitamin D3, KH1060, ED71, lα,24(S)-(OH)2 vitamin D3, and lα,24(R)-(OH)2 vitamin D3; (1) the dietary calcium supplement is selected from calcium carbonate, calcium citrate, and natural calcium salts; and
(m) the fluoride salts are selected from sodium fluoride and monosodium fluorophosphate (MFP); and pharmaceutically acceptable salts thereof.
25. The method of Claim 16 which further comprises the administration of alendronate monosodium trihydrate.
26. The method of Claim 20 which further comprises the administration of a bone-strengthening agent selected from the group consisting of:
(a) an estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative,
(b) a bisphosphonate,
(c) an antiestrogen or a selective estrogen receptor modulator, (d) an αvβ3 integrin receptor antagonist,
(e) a cathepsin K inhibitor,
(f) an HMG-CoA reductase inhibitor,
(g) an osteoclast vacuolar ATPase inhibitor,
(h) an antagonist of VEGF binding to osteoclast receptors, (i) an activator of peroxisome proliferator-activated receptor γ,
(j) calcitonin,
(k) a calcium receptor antagonist,
(1) parathyroid hormone or analog thereof,
(m) a growth hormone secretagogue, (n) human growth hormone, (o) insulin-like growth factor,
(p) a P-38 protein kinase inhibitor,
(q) bone morphogenetic protein,
(r ) an inhibitor of BMP antagonism, (s) a prostaglandin derivative,
(t) vitamin D or vitamin D derivative,
(u) vitamin K or vitamin K derivative,
(v) ipriflavone,
(w) fluoride salts, (x) dietary calcium supplement, and
(y) osteoprotegerin.
27. The method of Claim 26 wherein:
(a) the estrogen or estrogen derivative, alone or in combination with a progestin or progestin derivative, is selected from conjugated estrogen, equine estrogen,
17β-estradiol, estrone, 17β-ethynyl estradiol, alone or in combination with an agent selected from norethindrone and medroxyprogesterone acetate;
(b) the bisphosphonate is selected from:
( I ) (4-amino- l-hydroxybutylidene)-bisphosphonate; (2) [(cycloheptylamino)-methylene]-bisphosphonate;
(3) (dichloromethylene)-bisphosphonate;
(4) [ 1 -hydroxy-3 -(1 -pyrrolidinyl)-propylidene] -bisphosphonate ;
(5) (l-hydroxyethylidene)-bisphosphonate;
(6) [1 -hydroxy-3 -(methylpentylamino)propylidene] - bisphosphonate;
(7) (6-amino-l-hydroxyhexylidene)-bisphosphonate;
(8) [3-(dimethylamino)-l-hydroxypropylidene]- bisphosphonate;
(9) (3-amino- l-hydroxypropylidene)-bisphosphonate; (10) [2-(2-pyridinyl)ethylidene]-bisphosphonate;
(II) [l-hydroxy-2-(3-pyridinyl)-ethylidene]-bisphosphonate;
(12) { [(4-chlorophenyl)thio]methylene} -bisphosphonate;
(13) [l-hydroxy-2-(lH-imidazol-l-yl)ethylidene]- bisphosphonate; and (14) [l-hydroxy-2-imidazopyridin-(l,2-a)-3-ylethylidene]- bisphosphonate;
(c) the antiestrogen or selective estrogen receptor modulator is selected from the group consisting of raloxifene, clomiphene, zuclomiphene, enclomiphene, nafoxidene, CI-680, CI-628, CN-55,945-27, Mer-25, U-l 1 ,555A, U-100A, tamoxifen, lasofoxifene, toremifene, azorxifene, EM-800, EM-652, TSE 424, droloxifene, idoxifene, and levormeloxifene;
(d) the HMG-CoA reductase inhibitor is selected from lovastatin, simvastatin, dihydroxy-open acid simvastatin, pravastatin, fluvastatin, atorvastatin, cerivastatin, rosuvastatin, pitavastatin, and nisvastatin;
(e) calcitonin is salmon calcitonin administered as a nasal spray;
(f) bone morphogenetic protein is selected from BMP 2, BMP 3, BMP 5, BMP 6, BMP 7, TGF beta, and GDF5;
(g) insulin-like growth factor is selected from IGF I and IGF IT alone or in combination with IGF binding protein 3 ;
(h) the prostaglandin derivative is selected from agonists of prostaglandin receptors EP1, EP2, EP4, FP, and IP; (i) the fibroblast growth factor is selected from aFGF and bFGF; (j) parathyroid hormone (PTH) or PTH analog is selected from PTH subcutaneous injection, human PTH (1-84), human PTH (1-34), and other partial sequences, native or with substitutions; (k) vitamin D or vitamin D derivative is selected from natural vitamin D, 25-OH- vitamin D3, lα,25(OH)2 vitamin D3, lα-OH-vitamin D3, lα-OH-vitamin D2, dihydrotachysterol, 26,27-F6-lα,25(OH)2 vitamin D3, 19-nor-lα,25(OH)2 vitamin D3, 22-oxacalcitriol, calcipotriol, lα,25(OH)2-16-ene-23-yne-vitamin
D3 (Ro 23-7553), EB1089, 20-epi-lα,25(OH)2 vitamin D3, KH1060, ED71, lα,24(S)-(OH)2 vitamin D3, and lα,24(R)-(OH)2 vitamin D3; (1) the dietary calcium supplement is selected from calcium carbonate, calcium citrate, and natural calcium salts; and (m) the fluoride salts are selected from sodium fluoride and monosodium fluorophosphate (MFP); and pharmaceutically acceptable salts thereof.
28. The method of Claim 20 which further comprises the administration of alendronate monosodium trihydrate.
29. A composition comprising a compound according to Claim 1, or a pharmaceutically acceptable salt thereof, and a bone-strengthening agent selected from the group consisting of:
(a) an estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative,
(b) a bisphosphonate,
(c) an antiestrogen or a selective estrogen receptor modulator,
(d) an αvβ3 integrin receptor antagonist,
(e) a cathepsin K inhibitor, (f) an HMG-CoA reductase inhibitor,
(g) an osteoclast vacuolar ATPase inhibitor,
(h) an antagonist of VEGF binding to osteoclast receptors,
(i) an activator of peroxisome proliferator-activated receptor γ,
(j) calcitonin, (k) a calcium receptor antagonist,
(1) parathyroid hormone or analog thereof,
(m) a growth hormone secretagogue,
(n) human growth hormone,
(o) insulin-like growth factor, (p) a P-38 protein kinase inhibitor,
(q) bone morphogenetic protein,
(r) an inhibitor of BMP antagonism,
(s) a prostaglandin derivative,
(t) vitamin D or vitamin D derivative, (u) vitamin K or vitamin K derivative,
(v) ipriflavone,
(w) fluoride salts,
(x) dietary calcium supplement, and
(y) osteoprotegerin.
30. The use of a compound of structural formula I for the preparation of a medicament useful for modulating the androgen receptor in a tissue selective manner in a patient in need of such modulation.
31. The use of a compound according to Claim 1, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament useful for activating the function of the androgen receptor in a patient in need thereof.
32. The use of a compound according to Claim 1, or a pharmaceutically acceptable salt thereof, for the preparation of a medicament useful for treating a condition in a patient in need of such treatment which is caused by androgen deficiency or which can be ameliorated by androgen administration selected from the group consisting of osteoporosis, periodontal disease, bone fracture, bone damage following bone reconstructive surgery, sarcopenia, frailty, aging skin, male hypogonadism, female sexual dysfunction, post-menopausal symptoms in women, atherosclerosis, hypercholesterolemia, hyperlipidemia, obesity, aplastic anemia and other hematopoietic disorders, pancreatic cancer, renal cancer, prostate cancer, arthritis and joint repair.
33. The method of Claim 16 wherein the bone-strengthening agent is selected from the group consisting of:
(a) an estrogen or an estrogen derivative, alone or in combination with a progestin or progestin derivative, (b) a bisphosphonate,
(c) an antiestrogen or a selective estrogen receptor modulator,
(d) an αvβ3 integrin receptor antagonist,
(e) a cathepsin K inhibitor,
(f) an osteoclast vacuolar ATPase inhibitor, (g) calcitonin, and
(h) osteoprotegerin.
34. The method of Claim 20 wherein the bone-strengthening agent is selected from the group consisting of: (a) an estrogen or an estrogen derivative, alone or in combination with progestin or progestin derivative,
(b) a bisphosphonate,
(c) an antiestrogen or a selective estrogen receptor modulator,
(d) an αvβ3 integrin receptor antagonist, (e) a cathepsin K inhibitor, (f) an osteoclast vacuolar ATPase inhibitor,
(g) calcitonin, and (i) osteoprotegerin.
35. A compound of structural formula IT:
Figure imgf000132_0001
(N)
wherein
"a" represents a single bond or a double bond;
Rl is hydrogen, hydroxymethyl, or Cι_3 alkyl, wherein alkyl is unsubstituted or substituted with one to seven fluorine atoms;
R2 is hydrogen, fluorine, or Cι_4 alkyl when "a" represents a double bond; or two R2 substituents are each independently hydrogen, fluorine, or Cι_4 alkyl when "a" represents a single bond;
R4 is hydrogen, Cι_4 alkyl, C2-4 alkenyl, or C2-4 alkynyl; one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) Ci-8 alkyl,
(c) C2-8 alkenyl, wherein alkyl and alkenyl are unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci_4 alkoxy, Ci-4 alkoxyCi-4 alkoxy, and Ci_3 alkyloxycarbonyl;
(d) fluoro,
(e) cyano, (f) hydroxy,
(g) Ci-6 alkoxy,
(h) Ci-6 alkylcarbonyloxy, (i) Ci-6 alkylthio, (j) Ci-6 alkylsulfonyl, (k) C3_8 cycloalkyl Cθ-6 alkyl, (1) C3-8 cycloheteroalkyl Cθ-6 alkyl, (m) aryl Co-6 alkyl,
(n) aryl C2-4 alkenyl,
(0) amino,
(p) Ci-3 acylamino,
(q) aryl Cι_3 acylamino, (r) Ci-6 alkylamino,
(s) di(Ci-6 alkyl)amino,
(t) aryl-Cθ-3 alkylamino,
(u) di(aryl-Cθ-3 alkyl)amino,
(v) C3-6 cycloalkyl Cθ-2 alkylamino, (w) Ci-8 alkylsulfonylamino,
(x) aryl Cθ-3 alkylsulfonylamino,
(y) Ci-8 alkyloxycarbonylamino,
(z) aryl Cθ-3 alkyloxycarbonylamino,
(aa) aminocarbonylamino, (bb) Cl-8 alkylaminocarbonylamino,
(cc) aryl Cθ-3 alkylaminocarbonylamino, (dd) Cl-8 alkylaminocarbonyloxy, and (ee) aryl Cθ-3 alkylaminocarbonyloxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, and NCθ-4 alkyl; or R5 and R6 taken together can be =CR9RlO; wherein R and RlO are each independently selected from the group consisting of hydrogen, Ci_6 alkyl, C3-6 cycloalkyl Cθ-3 alkyl, C3-6 cycloheteroalkyl Cθ-3 alkyl, and aryl Cθ-3 alkyl; or R9 and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCι_4 alkyl, and NCi-4 alkyloxycarbonyl; wherein the aryl group in all instances above is selected from the group consisting of
(1) phenyl, (2) naphthyl,
(3) benzimidazolyl,
(4) benzofuranyl,
(5) benzothiophenyl, (6) benzoxazolyl,
(7) benzothiazolyl,
(26) benzodihydrofuranyl,
(27) 1,3-benzodioxolyl, (10) indolyl, (ll) quinolyl,
(12) isoquinolyl,
(13) furanyl,
(14) thienyl,
(15) imidazolyl, (16) oxazolyl,
(17) thiazolyl,
(18) isoxazolyl,
(19) isothiazolyl,
(20) pyrazolyl, (21) pyrrolyl,
(22) pyridyl,
(23) pyrimidyl,
(24) pyrazinyl,
(25) thiadiazolyl, (26) oxadiazolyl,
(27) triazolyl, and
(28) tetrazolyl; wherein the aryl group as defined in items (1) to (28) is unsubstituted or substituted with one to three groups independently selected from halogen, aryl, Cl-8 alkyl, C3-8 cycloalkyl, C3-8 cycloheteroalkyl, aryl Cι_6alkyl, amino Cθ-6alkyl, Ci-6 alkylamino Cθ-6alkyl, (Ci-6 alkyl)2amino Cθ-6alkyl, aryl Cθ-6 alkylamino Cθ-6alkyl, (aryl Cθ-6 alkyl)2amino Cθ-6alkyl, Cι_6 alkylthio, aryl Cθ-6alkylthio, Ci-6 alkylsulfinyl, aryl Cθ-6alkylsulfinyl, Ci-6 alkylsulfonyl, aryl Cθ-6alkylsulfonyl, Cι_6 alkoxy Cθ-6alkyl, aryl Cθ-6 alkoxy Cθ-6alkyl, hydroxycarbonyl Cθ-6alkyl, Ci-6 alkoxycarbonyl Cθ- 6alkyl, aryl CQ- alkoxycarbonyl Cθ-6alkyl, hydroxycarbonyl Ci-6 alkyloxy, hydroxy Cθ-6alkyl, cyano, nitro, perfluoroCi-4alkyl, perfluoroCι_4alkoxy, oxo, Ci_6 alkylcarbonyloxy, aryl Cθ-6alkylcarbonyloxy, Cι_6 alkylcarbonylamino, aryl Cθ-6 alkylcarbonylamino, Cι_6 alkylsulfonylamino, aryl Cθ-6alkylsulfonylamino, Ci_6 alkoxycarbonylamino, aryl Cθ-6 alkoxycarbonylamino, Ci-βalkylamino- carbonylamino, aryl Co-6alkylaminocarbonylamino, (Ci_6alkyl)2 aminocarbonylamino, (aryl Cθ-6alkyl)2 aminocarbonylamino, (Cι_6alkyl)2 aminocarbonyloxy, and (aryl Cθ-6alkyl)2 aminocarbonyloxy; with the provisos that (a) when "a" is a single or double bond, Rl is hydrogen or methyl, and R2, R5 and R6 are hydrogen, then R4 is not hydrogen, methyl, allyl, or n-propyl; and (b) when "a" is a single bond, Rl is methyl, and R and R6 are hydrogen, then R5 is not methyl, ethyl, isopropyl, or allyl.
36. The compound of Claim 35 wherein Rl is hydrogen or methyl and R4 is hydrogen.
37. The compound of Claim 35 wherein one of R5 and R6 is hydrogen or methyl and the other is selected from the group consisting of
(a) hydrogen,
(b) fluorine, (c) Ci-3 alkyl, wherein alkyl is unsubstituted or substituted one to three groups independently selected from amino, cyano, halogen, hydroxy, oxo, carboxy, Ci-4 alkoxy, Cι_3 alkoxy-Ci_3 alkoxy, and Cι_3 alkyloxycarbonyl; and
(d) arylmethyl, wherein aryl is selected from the group consisting of phenyl, naphthyl, pyridyl, furanyl, pyrrolyl, thiazolyl, imidazolyl, benzofuranyl, and 1,3- benzodioxolyl, wherein the aryl group is unsubstituted or substituted with one to two groups independently selected from halogen, Cι_4 alkyl, Ci_4 alkoxy, cyano, trifluoromethyl, and trifluoromethoxy; or R5 and R6 taken together with the carbon atom to which they are attached can form a C3-6 spirocyclic ring system optionally containing a heteroatom selected from O, S, NH, NCι_4 alkyl, and Ni-4 alkyloxycarbonyl; or R5 and R6 taken together can be =CR9R105 wherein R and RlO are each independently selected from the group consisting of hydrogen, Ci-6 alkyl, C3-6 cycloalkyl-Cθ-3 alkyl, C3-6 cycloheteroalkyl-Cθ-3 alkyl, aryl CQ-1 alkyl; or R and RlO taken together with the carbon atom to which they are attached can form a 3- to 6-membered ring optionally containing a heteroatom selected from O, S, NH, NCi-4 alkyl, and NCι_4 alkyloxycarbonyl.
38. The compound of Claim 37 wherein one of R5 and R6 is hydrogen and the other is arylmethyl wherein aryl is as defined in Claim 37.
39. The compound of Claim 38 wherein R5 is hydrogen and R6 is arylmethyl.
40. The compound of Claim 39 wherein Rl is hydrogen or methyl and R4 is hydrogen.
41. The compound of Claim 40 wherein "a" represents a double bond.
42. The compound of Claim 37 wherein R5 and R6 taken together are =CHaryl wherein aryl is as defined in Claim 37.
43. The compound of Claim 42 wherein Rl is hydrogen or methyl and R4 is hydrogen.
44. The compound of Claim 43 wherein "a" represents a double bond.
45. The compound of Claim 35 selected from the group consisting of:
16α-fluoro-l 7β-hydroxy-4-methyl-4-aza-5α-androst-l -en-3-one; 16β-fluoro- 17α-hydroxy-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 16α-fluoro- 17β-hydroxy-4, 17α-dimethyl-4-aza-5α-androst- 1 -en-3 -one; 16β-fluoro- 17β-hydroxy-4-mefhyl-4-aza-5α-androst- 1 -en-3 -one; 2α-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3 -one; 2β-fluoro- 17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; 2,2-difluoro-17β-hydroxy-4-methyl-4-aza-5α-androstan-3-one; 17β-hydroxy- 2α,4-dimethyl-4-aza-5α-androstan-3-one;
17β-hydroxy- 6α-(methoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(ethoxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-methoxyethyloxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(ethyloxycarbonylmethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(carboxymethyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-hydroxyethyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-allyl-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 4, 16α, 17α-trimethyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 7α-ethyl-4-methyl-4-aza-5α-androstan-3-one;
17β-hydroxy- 6α-(2-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(3-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(4-trifluoromethylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-fluorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(3-fluorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-(4-fluorobenzyl)-4-mefhyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6α-(3-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6α-(4-trifluoromethoxybenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6α-(3-methoxybenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-benzyl-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6α-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6α-(2-naphthyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6β-(2-methylbenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6β-(3 -methylbenzyl)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6β-(4-chlorobenzyl)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-chlorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-(3-fluorobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-(3-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3- one;
17β-hydroxy- 6-(2-trifluoromethylbenzylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(3-methoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3 ,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one; 17β-hydroxy- •16-(5-methoxy-3,4-methylenedioxybenzylidene)-4-methyl-4-aza-5α- androst-1-en- 3-one;
17β-hydroxy- 6-(3,5-difluorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(3,5-dichlorobenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-(3,4-dimethoxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(pyridin-4-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(cyclopropylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(furan-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy- 6-( 1 -methyl-imidazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 - en-3-one;
17β-hydroxy- 6-cyclohexylidene-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-ethylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-isopropylidene-4-methyl-4-aza-5α-androst-l-en-3-one; 17β-hydroxy- 6-isobutylidene-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 6-(piperidin-4-ylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(N-t-butyloxycarbonylpiperidin-4-ylidene)-4-methyl-4-aza-5α- androst-1-en- -one;
17β-hydroxy- 6-(l,3-thiazol-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3- one;
17β-hydroxy- 6-(benzofuran-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 6-(5-hydroxymethyl-furan-2-ylmethylidene)-4-methyl-4-aza-5α- androst-1-en- -one; 17β-hydroxy- 6-( 1 -methyl-pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-
3-one;
17β-hydroxy- 6- pyrrol-2-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3-one;
17β-hydroxy- 6-(2-ethoxy-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one; 17β-hydroxy- 6-(pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 6-(2-methyl-pyrimidin-5-ylmethylidene)-4-methyl-4-aza-5α-androst-l- en-3-one;
17β-hydroxy- 16-(3-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one; 17β-hydroxy- 16-(4-trifluoromethoxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3- one; 17β-hydroxy-l 6-(9-methoxy-quinolin-2-ylmethylidene)-4-methyl-4-aza-5α-androst-l - en-3-one;
17β-hydroxy- 16-(quinoxalin-6-ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-(quinoxalin-7-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(2-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; 17β-hydroxy-16-(3-hydroxybenzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(4-hydroxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy-16-(pyridin-3-ylmethylidene)-4-methyl-4-aza-5α-androst-l-en-3-one;
17β-hydroxy- 16-(N-methylpiperidin-4-ylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one; 17β-hydroxy-16-(2-chloro-4-dimethylaminobenzylidene)-4-methyl-4-aza-5α-androst- l-en-3-one;
17β-hydroxy- 16-(2-amino-pyridin-3 -ylmethylidene)-4-methyl-4-aza-5α-androst- 1 -en-
3-one;
17β-hydroxy- 16-(3-carboxymethyl-benzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 - one;
17β-hydroxy- 16-(3 -carboxybenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16-(3 -nitrobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one;
17β-hydroxy- 16-(4-nitrobenzylidene)-4-methyl-4-aza-5α-androst- 1 -en-3 -one; and
17β-hydroxy-16-(benzylidene)-4-methyl-4-aza-5α-androst-l-en-3-one; or a pharmaceutically acceptable salt thereof.
46. A composition comprising a compound of Claim 35 and a pharmaceutically acceptable carrier.
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2305352A1 (en) 2004-04-02 2011-04-06 Merck Sharp & Dohme Corp. 5-alpha-reductase inhibitors for use in the treatment of men with metabolic and anthropometric disorders
US8461102B2 (en) 2010-03-02 2013-06-11 George E. Royster, JR. Methods and compositions for treating and preventing symptoms of hormonal variations
US10342786B2 (en) 2017-10-05 2019-07-09 Fulcrum Therapeutics, Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD
US11291659B2 (en) 2017-10-05 2022-04-05 Fulcrum Therapeutics, Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2685942A1 (en) * 2007-05-16 2008-11-27 Merck & Co., Inc. Spiroindalones
WO2009136629A1 (en) * 2008-05-09 2009-11-12 あすか製薬株式会社 Prophylactic/therapeutic agent for lifestyle-related diseases

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693809A (en) * 1992-05-20 1997-12-02 Merck & Co., Inc. Substituted 4-aza-5α-androstan-ones as 5α-reductase inhibitors

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU527030B2 (en) * 1978-04-13 1983-02-10 Merck & Co., Inc. 4-aza-17-substituted-5a-androstan-3-ones
GB9002922D0 (en) * 1990-02-09 1990-04-04 Erba Carlo Spa 17 beta-substituted-4-aza-5 alpha-androstan-3-one derivatives and process for their preparation
US5710275A (en) 1992-05-20 1998-01-20 Merck & Co., Inc. 7β-substituted-4-aza-5α-androstan-3-ones as 5α-reductase inhibitors
KR950701527A (en) * 1992-05-21 1995-04-28 라브리 페르낭 INHIBITORS OF TESTOSTERONE 5α-REDUCTASE ACTIVITY
AU680818B2 (en) * 1992-05-21 1997-08-14 Endorecherche Inc. Improved antiandrogens
JP2741286B2 (en) * 1993-05-17 1998-04-15 アンドルシェルシュ・インコーポレイテッド Improved anti-androgen
US5550134A (en) * 1995-05-10 1996-08-27 Eli Lilly And Company Methods for inhibiting bone loss
WO1998025622A1 (en) * 1996-12-09 1998-06-18 Merck & Co., Inc. Methods and compositions for preventing and treating bone loss
AU5594398A (en) * 1996-12-09 1998-07-03 Merck & Co., Inc. Methods and compositions for preventing and treating bone loss
AU5369198A (en) * 1996-12-09 1998-07-03 Merck & Co., Inc. Methods and compositions for preventing and treating bone loss
US5945412A (en) * 1996-12-09 1999-08-31 Merck & Co., Inc. Methods and compositions for preventing and treating bone loss

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5693809A (en) * 1992-05-20 1997-12-02 Merck & Co., Inc. Substituted 4-aza-5α-androstan-ones as 5α-reductase inhibitors

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of EP1467739A2 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2305352A1 (en) 2004-04-02 2011-04-06 Merck Sharp & Dohme Corp. 5-alpha-reductase inhibitors for use in the treatment of men with metabolic and anthropometric disorders
US8461102B2 (en) 2010-03-02 2013-06-11 George E. Royster, JR. Methods and compositions for treating and preventing symptoms of hormonal variations
US10342786B2 (en) 2017-10-05 2019-07-09 Fulcrum Therapeutics, Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD
US10537560B2 (en) 2017-10-05 2020-01-21 Fulcrum Therapeutics. Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD
US11291659B2 (en) 2017-10-05 2022-04-05 Fulcrum Therapeutics, Inc. P38 kinase inhibitors reduce DUX4 and downstream gene expression for the treatment of FSHD
US11479770B2 (en) 2017-10-05 2022-10-25 Fulcrum Therapeutics, Inc. Use of p38 inhibitors to reduce expression of DUX4

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AU2003219656B2 (en) 2007-05-17
AU2003219656A1 (en) 2003-07-30
EP1467739A2 (en) 2004-10-20
US20050107416A1 (en) 2005-05-19
EP1467739A4 (en) 2008-01-09
US7605152B2 (en) 2009-10-20
CA2472475C (en) 2010-05-18
CA2472475A1 (en) 2003-07-24
JP2005518404A (en) 2005-06-23
JP4563032B2 (en) 2010-10-13

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