WO2003057830A3 - Compositions and methods for hydroxylating epothilones - Google Patents

Compositions and methods for hydroxylating epothilones Download PDF

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Publication number
WO2003057830A3
WO2003057830A3 PCT/US2002/040359 US0240359W WO03057830A3 WO 2003057830 A3 WO2003057830 A3 WO 2003057830A3 US 0240359 W US0240359 W US 0240359W WO 03057830 A3 WO03057830 A3 WO 03057830A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
epothilone
epothilones
hydroxylase
hydroxylating
Prior art date
Application number
PCT/US2002/040359
Other languages
French (fr)
Other versions
WO2003057830A2 (en
Inventor
Jonathan David Basch
Shu-Jen Chiang
Suo-Win Liu
Akbar Nayeem
Yuhua Sun
You Li
Original Assignee
Bristol Myers Squibb Co
Jonathan David Basch
Shu-Jen Chiang
Suo-Win Liu
Akbar Nayeem
Yuhua Sun
You Li
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bristol Myers Squibb Co, Jonathan David Basch, Shu-Jen Chiang, Suo-Win Liu, Akbar Nayeem, Yuhua Sun, You Li filed Critical Bristol Myers Squibb Co
Priority to JP2003558132A priority Critical patent/JP2005514031A/en
Priority to AU2002357872A priority patent/AU2002357872A1/en
Priority to CA002471874A priority patent/CA2471874A1/en
Priority to EP02792416A priority patent/EP1465908A4/en
Publication of WO2003057830A2 publication Critical patent/WO2003057830A2/en
Publication of WO2003057830A3 publication Critical patent/WO2003057830A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/18—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin
    • C12P17/181—Heterocyclic compounds containing oxygen atoms as the only ring heteroatoms in the condensed system, e.g. Salinomycin, Septamycin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes

Abstract

Isolated nucleic acid sequences and polypeptides encoded thereby for epothilone B hydroxylase and mutants and variants thereof and a ferredoxin located downstream from the epothilone B hydroxylase gene are provided. Also provided are vectors and cells containing these vectors. In addition, methods for producing recombinant microorganisms, methods for using these recombinant microorganism to produce hydroxyalkyl-bearing epothilones and an epothilone analog produced by a mutant of epothilone B hydroxylase are provided.
PCT/US2002/040359 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilones WO2003057830A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2003558132A JP2005514031A (en) 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilone
AU2002357872A AU2002357872A1 (en) 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilones
CA002471874A CA2471874A1 (en) 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilones
EP02792416A EP1465908A4 (en) 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilones

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34427101P 2001-12-26 2001-12-26
US60/344,271 2001-12-26

Publications (2)

Publication Number Publication Date
WO2003057830A2 WO2003057830A2 (en) 2003-07-17
WO2003057830A3 true WO2003057830A3 (en) 2004-08-05

Family

ID=23349792

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/040359 WO2003057830A2 (en) 2001-12-26 2002-12-17 Compositions and methods for hydroxylating epothilones

Country Status (6)

Country Link
EP (1) EP1465908A4 (en)
JP (1) JP2005514031A (en)
AU (1) AU2002357872A1 (en)
CA (1) CA2471874A1 (en)
TW (1) TW200301697A (en)
WO (1) WO2003057830A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE41990E1 (en) 1996-12-03 2010-12-07 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto, analogues and uses thereof

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6884608B2 (en) * 2001-12-26 2005-04-26 Bristol-Myers Squibb Company Compositions and methods for hydroxylating epothilones
DE60326832D1 (en) * 2002-04-12 2009-05-07 Mercian Corp EXPRESSION SYSTEM OF CYTOCHROM P-450 GENERATING FROM ACTINOMYCETE IN ESCHERICHIA COLI
US11384371B2 (en) 2016-11-15 2022-07-12 Hypha Discovery Limited Hydroxylation of branched aliphatic or aromatic substrates employing the amycolatopsis lurida cytochrome P450
GB201807815D0 (en) 2018-05-14 2018-06-27 Hypha Discovery Ltd Hydroxylation techniques
GB201819209D0 (en) 2018-11-26 2019-01-09 Hypha Discovery Ltd Biocatalytic techniques

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219877A1 (en) * 2001-11-15 2003-11-27 Li Tang Novel epothilone compounds and methods for making the same

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HUP0200296A2 (en) * 1998-12-23 2002-05-29 Bristol Myers Squibb Co Microbial transformation method for the preparation of an epothilone

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030219877A1 (en) * 2001-11-15 2003-11-27 Li Tang Novel epothilone compounds and methods for making the same

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BETLACH ET AL: "Characterization of the macrolide P-450 hydroxylase from Streptomyces venezuelae which converts narbomycin to picromycin", BIOCHEMISTRY, vol. 37, 1998, pages 14937 - 14942, XP002117398 *
HALPERT ET AL: "Structure-function of cytochromes P450 and flavin-containing monooxygenases", DRUG METABOLISM AND DISPOSITION, vol. 26, no. 12, 1998, pages 1223 - 1231, XP002979013 *
TANG ET AL: "Cloning and heterologous expression of the epothilone gene cluster", SCIENCE, vol. 287, January 2000 (2000-01-01), pages 640 - 642, XP002135841 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USRE41990E1 (en) 1996-12-03 2010-12-07 Sloan-Kettering Institute For Cancer Research Synthesis of epothilones, intermediates thereto, analogues and uses thereof

Also Published As

Publication number Publication date
JP2005514031A (en) 2005-05-19
WO2003057830A2 (en) 2003-07-17
EP1465908A2 (en) 2004-10-13
TW200301697A (en) 2003-07-16
EP1465908A4 (en) 2005-11-30
AU2002357872A1 (en) 2003-07-24
CA2471874A1 (en) 2003-07-17

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