WO2003054224A3 - Method and integrated device for the detection of cytosine methylations - Google Patents

Method and integrated device for the detection of cytosine methylations Download PDF

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Publication number
WO2003054224A3
WO2003054224A3 PCT/DE2002/004507 DE0204507W WO03054224A3 WO 2003054224 A3 WO2003054224 A3 WO 2003054224A3 DE 0204507 W DE0204507 W DE 0204507W WO 03054224 A3 WO03054224 A3 WO 03054224A3
Authority
WO
WIPO (PCT)
Prior art keywords
dna
amplified
primers
domain located
amplification
Prior art date
Application number
PCT/DE2002/004507
Other languages
German (de)
French (fr)
Other versions
WO2003054224A2 (en
Inventor
Kurt Berlin
Original Assignee
Epigenomics Ag
Kurt Berlin
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Epigenomics Ag, Kurt Berlin filed Critical Epigenomics Ag
Priority to EP02791612A priority Critical patent/EP1451359A2/en
Priority to AU2002357968A priority patent/AU2002357968A1/en
Publication of WO2003054224A2 publication Critical patent/WO2003054224A2/en
Publication of WO2003054224A3 publication Critical patent/WO2003054224A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification

Abstract

The invention relates to a method for detecting DNA polymorphisms and cytosine methylation, consisting of the following steps: a) DNA is extracted from a sample for analysis; b) if a cytosine methylation analysis is carried out, the DNA is chemically and/or enzymatically treated; c) the DNA is amplified in a polymerase reaction using a dissolved primer, and another primer is attached to the surface of a solid phase. At least one of the primers consists of two domains. The domain located at the 3' end hybridizes with the DNA, whereas the domain located at the 5' end does not hybridize; d) the DNA amplified in step c) is amplified once again. The primers of the second amplification hybridize with the domain located at the 5' end of at least one of the first primers or are identical therewith. The primers used for the second amplification contain a detectable marker; e) the amplified substances are hybridized with oligonucleotides and/or PNA oligomers that are bound to a solid phase which does not act as a primer during amplification. Sequential features or methylation patterns are detected by means of the hybridization.
PCT/DE2002/004507 2001-12-05 2002-12-05 Method and integrated device for the detection of cytosine methylations WO2003054224A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP02791612A EP1451359A2 (en) 2001-12-05 2002-12-05 Method and integrated device for the detection of cytosine methylations
AU2002357968A AU2002357968A1 (en) 2001-12-05 2002-12-05 Method and integrated device for the detection of cytosine methylations

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10160983A DE10160983B4 (en) 2001-12-05 2001-12-05 Method and integrated device for the detection of cytosine methylation
DE10160983.3 2001-12-05

Publications (2)

Publication Number Publication Date
WO2003054224A2 WO2003054224A2 (en) 2003-07-03
WO2003054224A3 true WO2003054224A3 (en) 2003-10-30

Family

ID=7708910

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2002/004507 WO2003054224A2 (en) 2001-12-05 2002-12-05 Method and integrated device for the detection of cytosine methylations

Country Status (4)

Country Link
EP (1) EP1451359A2 (en)
AU (1) AU2002357968A1 (en)
DE (1) DE10160983B4 (en)
WO (1) WO2003054224A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10236406C1 (en) * 2002-08-02 2003-12-24 Epigenomics Ag Method for the amplification of nucleic acids of low complexity
DE602004004988T2 (en) * 2003-12-16 2008-01-10 Bayer Healthcare, Llc Methylation Status Detection Assays Using Methylation-Specific Primer Extension (MSPE)
WO2007032748A1 (en) * 2005-09-15 2007-03-22 Agency For Science, Technology & Research Method for detecting dna methylation
AT502549B1 (en) 2005-10-07 2007-06-15 Anagnostics Bioanalysis Gmbh DEVICE FOR THE ANALYSIS OF LIQUID SAMPLES

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
US6017738A (en) * 1991-11-01 2000-01-25 Adelaide Children's Hospital Solid phase amplification process
WO2000063437A2 (en) * 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
DE19952723A1 (en) * 1999-10-26 2001-05-10 Epigenomics Ag Device and method for hybridizing double-stranded DNA samples on oligomer arrays
WO2001062064A2 (en) * 2000-02-25 2001-08-30 Epigenomics Ag Method for detecting cytosine methylation in dna samples
US6310354B1 (en) * 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
US20010036632A1 (en) * 1999-12-29 2001-11-01 Zailin Yu Methods for amplifying and detecting multiple polynucleotides on a solid phase support

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19934084A1 (en) * 1999-07-15 2001-01-18 Universitaetsklinikum Charite Labeling DNA fragments during polymerase chain reaction, useful e.g. for sequencing or polymorphic analysis, uses labeled universal primer and sequence-specific primers
DE19935772C2 (en) * 1999-07-26 2002-11-07 Epigenomics Ag Method for the relative quantification of the methylation of cytosine bases in DNA samples

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5629158A (en) * 1989-03-22 1997-05-13 Cemu Bitecknik Ab Solid phase diagnosis of medical conditions
WO1993004199A2 (en) * 1991-08-20 1993-03-04 Scientific Generics Limited Methods of detecting or quantitating nucleic acids and of producing labelled immobilised nucleic acids
US6017738A (en) * 1991-11-01 2000-01-25 Adelaide Children's Hospital Solid phase amplification process
US6310354B1 (en) * 1996-12-03 2001-10-30 Erkki Soini Method and a device for monitoring nucleic acid amplification reactions
WO2000063437A2 (en) * 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
DE19952723A1 (en) * 1999-10-26 2001-05-10 Epigenomics Ag Device and method for hybridizing double-stranded DNA samples on oligomer arrays
US20010036632A1 (en) * 1999-12-29 2001-11-01 Zailin Yu Methods for amplifying and detecting multiple polynucleotides on a solid phase support
WO2001062064A2 (en) * 2000-02-25 2001-08-30 Epigenomics Ag Method for detecting cytosine methylation in dna samples

Also Published As

Publication number Publication date
EP1451359A2 (en) 2004-09-01
WO2003054224A2 (en) 2003-07-03
DE10160983A1 (en) 2003-06-26
AU2002357968A1 (en) 2003-07-09
DE10160983B4 (en) 2004-12-09

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