WO2003052136A3 - Sequenzierung über lochmembranen - Google Patents

Sequenzierung über lochmembranen Download PDF

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Publication number
WO2003052136A3
WO2003052136A3 PCT/EP2002/014489 EP0214489W WO03052136A3 WO 2003052136 A3 WO2003052136 A3 WO 2003052136A3 EP 0214489 W EP0214489 W EP 0214489W WO 03052136 A3 WO03052136 A3 WO 03052136A3
Authority
WO
WIPO (PCT)
Prior art keywords
sequencing
perforated membranes
perforated
membranes
nucleic acid
Prior art date
Application number
PCT/EP2002/014489
Other languages
English (en)
French (fr)
Other versions
WO2003052136A2 (de
Inventor
Rudolf Rigler
Horst Vogel
Original Assignee
Gnothis Holding Sa
Rudolf Rigler
Horst Vogel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gnothis Holding Sa, Rudolf Rigler, Horst Vogel filed Critical Gnothis Holding Sa
Priority to EP02793068A priority Critical patent/EP1458890A2/de
Priority to US10/503,562 priority patent/US20050130159A1/en
Priority to AU2002358756A priority patent/AU2002358756A1/en
Publication of WO2003052136A2 publication Critical patent/WO2003052136A2/de
Publication of WO2003052136A3 publication Critical patent/WO2003052136A3/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Die Erfindung betrifft ein Verfahren und eine Vorrichtung zur Sequenzierung von Nukleinsäuremolekülen über eine Lochmembran. Insbesondere kann die Erfindung zur Multiplex-Sequenzierung eingesetzt werden.
PCT/EP2002/014489 2001-12-19 2002-12-18 Sequenzierung über lochmembranen WO2003052136A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP02793068A EP1458890A2 (de) 2001-12-19 2002-12-18 Sequenzierung über lochmembranen
US10/503,562 US20050130159A1 (en) 2001-12-19 2002-12-18 Sequencing on perforated membranes
AU2002358756A AU2002358756A1 (en) 2001-12-19 2002-12-18 Sequencing on perforated membranes

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10162535A DE10162535A1 (de) 2001-12-19 2001-12-19 Sequenzierung über Lochmembranen
DE10162535.9 2001-12-19

Publications (2)

Publication Number Publication Date
WO2003052136A2 WO2003052136A2 (de) 2003-06-26
WO2003052136A3 true WO2003052136A3 (de) 2004-01-15

Family

ID=7709894

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/014489 WO2003052136A2 (de) 2001-12-19 2002-12-18 Sequenzierung über lochmembranen

Country Status (5)

Country Link
US (1) US20050130159A1 (de)
EP (1) EP1458890A2 (de)
AU (1) AU2002358756A1 (de)
DE (1) DE10162535A1 (de)
WO (1) WO2003052136A2 (de)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102004038359A1 (de) * 2004-08-06 2006-03-16 Rudolf Rigler Paralleles Hochdurchsatz-Einzelmolekül-Sequenzierungsverfahren
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
CN102124112B (zh) 2008-09-10 2013-03-20 金斯瑞公司 基于同源重组dna的克隆方法及组合物
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
CN105283560B (zh) 2013-05-24 2018-11-30 昆塔波尔公司 基于纳米孔的通过混合的fret检测的核酸分析
JP6740222B2 (ja) 2014-10-10 2020-08-12 クアンタポール， インコーポレイテッド 互いに消光する蛍光標識とのナノポアを基礎にしたポリマー分析
US9624537B2 (en) 2014-10-24 2017-04-18 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
EP3482196B1 (de) 2016-07-05 2022-02-23 Quantapore, Inc. Optisch basierte nanoporensequenzierung

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6074823A (en) * 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
WO2002002795A2 (de) * 2000-06-30 2002-01-10 Gnothis Holding Sa Multiplex-sequenzierungsverfahren

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6197557B1 (en) * 1997-03-05 2001-03-06 The Regents Of The University Of Michigan Compositions and methods for analysis of nucleic acids
US6245506B1 (en) * 1997-07-30 2001-06-12 Bbi Bioseq, Inc. Integrated sequencing device

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6074823A (en) * 1993-03-19 2000-06-13 Sequenom, Inc. DNA sequencing by mass spectrometry via exonuclease degradation
WO2002002795A2 (de) * 2000-06-30 2002-01-10 Gnothis Holding Sa Multiplex-sequenzierungsverfahren

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
DÖRRE K ET AL.: "Techniques for single molecule sequencing", BIOIMAGING, vol. 5, 1997, pages 139 - 152, XP002260686 *
HINZ M ET AL: "Polymer support for exonucleolytic sequencing", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 86, no. 3, 13 April 2001 (2001-04-13), pages 281 - 288, XP004230521, ISSN: 0168-1656 *
SAUER M ET AL: "Single molecule DNA sequencing in submicrometer channels: state of the art and future prospects", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 86, no. 3, 13 April 2001 (2001-04-13), pages 181 - 201, XP004230515, ISSN: 0168-1656 *
STEPHAN J ET AL: "Towards a general procedure for sequencing single DNA molecules", JOURNAL OF BIOTECHNOLOGY, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 86, no. 3, 13 April 2001 (2001-04-13), pages 255 - 267, XP004230519, ISSN: 0168-1656 *

Also Published As

Publication number Publication date
AU2002358756A8 (en) 2003-06-30
EP1458890A2 (de) 2004-09-22
US20050130159A1 (en) 2005-06-16
WO2003052136A2 (de) 2003-06-26
AU2002358756A1 (en) 2003-06-30
DE10162535A1 (de) 2003-07-03

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