WO2003052134A3 - Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof - Google Patents

Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof Download PDF

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Publication number
WO2003052134A3
WO2003052134A3 PCT/DK2002/000877 DK0200877W WO03052134A3 WO 2003052134 A3 WO2003052134 A3 WO 2003052134A3 DK 0200877 W DK0200877 W DK 0200877W WO 03052134 A3 WO03052134 A3 WO 03052134A3
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WO
WIPO (PCT)
Prior art keywords
nucleic acid
oligonucleotides
target sequence
methods
nucleic acids
Prior art date
Application number
PCT/DK2002/000877
Other languages
French (fr)
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WO2003052134A2 (en
Inventor
Ulf Bech Christensen
Erik Bjerregaard Pedersen
Original Assignee
Ulf Bech Christensen
Erik Bjerregaard Pedersen
Unest As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DKPA200101897 priority Critical
Priority to DKPA200101899 priority
Priority to DKPA200101900 priority
Priority to DKPA200101897 priority
Priority to DKPA200101898 priority
Priority to DKPA200101900 priority
Priority to DKPA200101899 priority
Priority to DKPA200101898 priority
Priority to US60/365,545 priority
Priority to US36554502P priority
Priority to DKPA200201578 priority
Priority to DKPA200201575 priority
Priority to DKPA200201577 priority
Priority to DKPA200201577 priority
Priority to DKPA200201575 priority
Priority to DKPA200201578 priority
Priority to DKPA200201576 priority
Priority to DKPA200201576 priority
Application filed by Ulf Bech Christensen, Erik Bjerregaard Pedersen, Unest As filed Critical Ulf Bech Christensen
Publication of WO2003052134A2 publication Critical patent/WO2003052134A2/en
Publication of WO2003052134A3 publication Critical patent/WO2003052134A3/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6832Enhancement of hybridisation reaction
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
    • A61K38/00Medicinal preparations containing peptides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/13Decoys
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/31Chemical structure of the backbone
    • C12N2310/318Chemical structure of the backbone where the PO2 is completely replaced, e.g. MMI or formacetal
    • C12N2310/3181Peptide nucleic acid, PNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/32Chemical structure of the sugar
    • C12N2310/3212'-O-R Modification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/32Chemical structure of the sugar
    • C12N2310/323Chemical structure of the sugar modified ring structure
    • C12N2310/3231Chemical structure of the sugar modified ring structure having an additional ring, e.g. LNA, ENA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/35Nature of the modification
    • C12N2310/351Conjugate
    • C12N2310/3511Conjugate intercalating or cleaving agent
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/30Chemical structure
    • C12N2310/35Nature of the modification
    • C12N2310/351Conjugate
    • C12N2310/3517Marker; Tag

Abstract

The present invention relates to oligonucleotides or oligonucleotide analogues comprising at least two intercalator pseudonucleotides. Intercalator pseudonucleotides are capable of being incorporated into the backdone of a nucleic acid analogue and comprise an intercalator comprising at least one essentially flat conjugated system, which is capable of co-stacking with nucleobases of a nucleic acid. Furthermore, the invention relates to methods of detecting nucleic acids or nucleic acid analogues comprising a specific target sequence, methods of differentiating between a nucleic acid or nucleic acid analogue comprising a specific target sequence and a nucleic acid comprising a mutant sequence which differs from the target sequence by at least one nucleobase, and methods for detecting a target sequence and/or a mutant sequence, which offer from the target sequence by at least one nucleobase. In preferred embodiments of the invention said methods involve the use of oligonucleotides or oligonucleotide analogues comprising at least two intercalator pseudonucleotides.
PCT/DK2002/000877 2001-12-18 2002-12-18 Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof WO2003052134A2 (en)

Priority Applications (18)

Application Number Priority Date Filing Date Title
DKPA200101899 2001-12-18
DKPA200101900 2001-12-18
DKPA200101897 2001-12-18
DKPA200101898 2001-12-18
DKPA200101900 2001-12-18
DKPA200101897 2001-12-18
DKPA200101899 2001-12-18
DKPA200101898 2001-12-18
US36554502P true 2002-03-20 2002-03-20
US60/365,545 2002-03-20
DKPA200201575 2002-10-14
DKPA200201577 2002-10-14
DKPA200201577 2002-10-14
DKPA200201575 2002-10-14
DKPA200201578 2002-10-14
DKPA200201576 2002-10-14
DKPA200201576 2002-10-14
DKPA200201578 2002-10-14

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU2002361946A AU2002361946A1 (en) 2001-12-18 2002-12-18 Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof

Publications (2)

Publication Number Publication Date
WO2003052134A2 WO2003052134A2 (en) 2003-06-26
WO2003052134A3 true WO2003052134A3 (en) 2004-03-25

Family

ID=27576036

Family Applications (4)

Application Number Title Priority Date Filing Date
PCT/DK2002/000877 WO2003052134A2 (en) 2001-12-18 2002-12-18 Oligonucleotides comprising intercalator pseudonucleotide(s) for detection of nucleic acids and mutants hereof
PCT/DK2002/000875 WO2003052133A2 (en) 2001-12-18 2002-12-18 Oligonucleotide analogues comprising intercalator pseudonucleotides
PCT/DK2002/000876 WO2003051901A2 (en) 2001-12-18 2002-12-18 Pseudonucleotide comprising an intercalator
PCT/DK2002/000874 WO2003052132A2 (en) 2001-12-18 2002-12-18 Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides

Family Applications After (3)

Application Number Title Priority Date Filing Date
PCT/DK2002/000875 WO2003052133A2 (en) 2001-12-18 2002-12-18 Oligonucleotide analogues comprising intercalator pseudonucleotides
PCT/DK2002/000876 WO2003051901A2 (en) 2001-12-18 2002-12-18 Pseudonucleotide comprising an intercalator
PCT/DK2002/000874 WO2003052132A2 (en) 2001-12-18 2002-12-18 Linear and hairpin oligonucleotide analogues comprising intercalator pseudonucleotides

Country Status (4)

Country Link
EP (1) EP1468007A2 (en)
CN (1) CN1653079B (en)
AU (4) AU2002358463A1 (en)
WO (4) WO2003052134A2 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003900368A0 (en) * 2003-01-24 2003-02-13 Human Genetic Signatures Pty Ltd Assay for nucleic acid molecules
EP1594987B1 (en) 2003-02-21 2009-05-27 GeneForm Technologies Limited Nucleic acid sequencing methods, kits and reagents
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2004101816A1 (en) * 2003-05-15 2004-11-25 Human Genetic Signatures Pty Ltd Decoys for nucleic acid-binding proteins
DE602004028478D1 (en) 2003-06-17 2010-09-16 Human Genetic Signatures Pty A process for genomamplifikation
DE602004018801D1 (en) 2003-09-04 2009-02-12 Human Genetic Signatures Pty Nucleic acid detection test
JP4397659B2 (en) * 2003-09-11 2010-01-13 独立行政法人科学技術振興機構 dna detection method using molecular beacons using switching monomer emission and excimer emission of the fluorescent molecule
DK1794173T3 (en) 2004-09-10 2010-10-25 Human Genetic Signatures Pty Amplification blocker comprising intercalating nucleic acids (INA) containing intercalating pseudonucleotides (IPN)
EP1799853A2 (en) 2004-09-24 2007-06-27 Universität Bern Molecular beacons
BRPI0517131A2 (en) 2004-12-03 2008-12-30 Human Genetic Signatures Pty methods to simplify microbial nucleic acids by modificaÇço chemistry cytosine
SI1888748T1 (en) * 2005-05-25 2014-02-28 Tina Holding Aps Stable and selective formation of hoogsteen-type triplexes and duplexes using twisted intercalating nucleic acids (tina) and process for the preparation of tina
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
WO2007030882A1 (en) 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for a health state
NZ571966A (en) 2006-03-16 2011-09-30 Pentabase Aps Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids
US8299237B2 (en) 2007-08-30 2012-10-30 Hadasit Medical Research Services & Development Ltd. Nucleic acid sequences comprising NF-κB binding site within O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter region and uses thereof for the treatment of cancer and immune-related disorders
EP2215250B1 (en) 2007-11-27 2013-02-27 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
AU2008338530A1 (en) 2007-12-14 2009-06-25 Minitube Of America, Inc. Gender-specific separation of sperm cells and embryos
WO2011032034A2 (en) 2009-09-10 2011-03-17 University Of Idaho Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids
SG184946A1 (en) 2010-05-07 2012-11-29 Quantibact As Method for generating a double stranded nucleic acid with a single stranded overhang
MX2014000797A (en) 2011-07-19 2014-07-09 Univ Idaho Embodiments of a probe and method for targeting nucleic acids.
NZ622418A (en) 2011-09-07 2014-12-24 Human Genetic Signatures Pty Molecular detection assay
EP3350347A1 (en) * 2015-09-14 2018-07-25 Pentabase APS Methods and materials for detection of mutations

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5332659A (en) * 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
US5414077A (en) * 1990-02-20 1995-05-09 Gilead Sciences Non-nucleoside linkers for convenient attachment of labels to oligonucleotides using standard synthetic methods
WO1997039008A1 (en) * 1996-04-12 1997-10-23 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
WO1997043298A1 (en) * 1996-05-15 1997-11-20 Research Corporation Technologies, Inc. Novel nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
WO1998018965A1 (en) * 1996-10-29 1998-05-07 University Of Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US6225052B1 (en) * 1997-02-24 2001-05-01 Roche Diagnostics Gmbh Method for determining a nucleic acid

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3293820B2 (en) * 1985-12-13 2002-06-17 エンゾ− バイオケム インコ−ポレイテツド The novel one-step method and the polynucleotide compound to Haiburitsudo formed to a target polynucleotide
US5585481A (en) * 1987-09-21 1996-12-17 Gen-Probe Incorporated Linking reagents for nucleotide probes
US6031091A (en) * 1987-09-21 2000-02-29 Gen-Probe Incorporated Non-nucleotide linking reagents for nucleotide probes
US5998135A (en) * 1989-02-24 1999-12-07 Enzo Diagnostics, Inc. Energy transfer hybridization assay using intercalators and lanthanide metals
US5886177A (en) * 1994-01-11 1999-03-23 Isis Pharmaceuticals, Inc. Phosphate linked oligomers
GB9604669D0 (en) * 1996-03-05 1996-05-01 Ciba Geigy Ag Chemical compounds
US6172209B1 (en) * 1997-02-14 2001-01-09 Isis Pharmaceuticals Inc. Aminooxy-modified oligonucleotides and methods for making same
US6506567B2 (en) * 2000-01-31 2003-01-14 Fuji Photo Film Co., Ltd. Water-soluble flourescent intercalator compound
US20030165837A1 (en) * 2000-06-14 2003-09-04 The Scripps Research Institute Method for screening DNA binding

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5414077A (en) * 1990-02-20 1995-05-09 Gilead Sciences Non-nucleoside linkers for convenient attachment of labels to oligonucleotides using standard synthetic methods
US5332659A (en) * 1992-04-09 1994-07-26 The United States Of America As Represented By The Secretary Of The Navy Light emission-or absorbance-based binding assays for polynucleic acids
WO1997039008A1 (en) * 1996-04-12 1997-10-23 The Public Health Research Institute Of The City Of New York, Inc. Detection probes, kits and assays
WO1997043298A1 (en) * 1996-05-15 1997-11-20 Research Corporation Technologies, Inc. Novel nucleoside analogs with polycyclic aromatic groups attached, methods of synthesis and uses therefor
WO1998018965A1 (en) * 1996-10-29 1998-05-07 University Of Nebraska At Lincoln Method for detecting point mutations in dna utilizing fluorescence energy transfer
US6225052B1 (en) * 1997-02-24 2001-05-01 Roche Diagnostics Gmbh Method for determining a nucleic acid

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHRISTENSEN U B ET AL: "Intercalating nucleic acids containing insertions of 1-O-(1-pyrenylmethyl)glycerol: stabilisation of dsDNA and discrimination of DNA over RNA", NUCLEIC ACIDS RESEARCH, OXFORD UNIVERSITY PRESS, SURREY, GB, vol. 30, no. 22, 15 November 2002 (2002-11-15), pages 4918 - 4925, XP002246723, ISSN: 0305-1048 *
DE MESMAEKER A ET AL: "Amide Backbone Modifications for Antisense Oligonucleotides Carrying Potential Intercalating Substituents: Influence on the Thermodynamic Stability of the Corresponding Duplexes with RNA- and DNA-Complements", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, OXFORD, GB, vol. 7, no. 14, 22 July 1997 (1997-07-22), pages 1869 - 1874, XP004136348, ISSN: 0960-894X *
YAMANA K ET AL: "Oligonucleotides Having Covalently Linked Anthracene at Specific Sugar Residue: Differential Binding to DNA and RNA and Fluorescence Properties", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 36, no. 46, 13 November 1995 (1995-11-13), pages 8427 - 8430, XP004026928, ISSN: 0040-4039 *
YAMANA K ET AL: "Synthesis of oligonucleotide derivatives containing pyrene labeled glycerol linkers: enhanced excimer fluorescence on binding to a complementary DNA sequence", TETRAHEDRON LETTERS, ELSEVIER SCIENCE PUBLISHERS, AMSTERDAM, NL, vol. 38, no. 34, 1 August 1997 (1997-08-01), pages 6051 - 6054, XP004094186, ISSN: 0040-4039 *

Also Published As

Publication number Publication date
WO2003052133A3 (en) 2003-10-02
WO2003052133A2 (en) 2003-06-26
WO2003051901A2 (en) 2003-06-26
WO2003051901A3 (en) 2003-11-27
AU2002358463A1 (en) 2003-06-30
AU2002358464B2 (en) 2006-04-27
EP1468007A2 (en) 2004-10-20
AU2002358464A1 (en) 2003-06-30
AU2002361946A1 (en) 2003-06-30
AU2002361946A8 (en) 2003-06-30
AU2002358463A8 (en) 2003-06-30
AU2002358462A8 (en) 2003-06-30
AU2002358462A1 (en) 2003-06-30
CN1653079B (en) 2010-06-16
CN1653079A (en) 2005-08-10
WO2003052132A3 (en) 2003-10-09
WO2003052132A2 (en) 2003-06-26
WO2003052134A2 (en) 2003-06-26

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