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WO2003043713A1 - Size-exclusion filtering device, method for making same and resulting filtering method - Google Patents

Size-exclusion filtering device, method for making same and resulting filtering method

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Publication number
WO2003043713A1
WO2003043713A1 PCT/FR2002/003990 FR0203990W WO03043713A1 WO 2003043713 A1 WO2003043713 A1 WO 2003043713A1 FR 0203990 W FR0203990 W FR 0203990W WO 03043713 A1 WO03043713 A1 WO 03043713A1
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WO
Grant status
Application
Patent type
Prior art keywords
material
filter
device
filtration
gel
Prior art date
Application number
PCT/FR2002/003990
Other languages
French (fr)
Inventor
Joël LESSER
Original Assignee
Adiatec
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/291Gel sorbents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D15/00Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08Selective adsorption, e.g. chromatography
    • B01D15/26Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/34Size selective separation, e.g. size exclusion chromatography, gel filtration, permeation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26Synthetic macromolecular compounds
    • B01J20/261Synthetic macromolecular compounds obtained by reactions only involving carbon to carbon unsaturated bonds
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/22Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising organic material
    • B01J20/26Synthetic macromolecular compounds
    • B01J20/262Synthetic macromolecular compounds obtained otherwise than by reactions only involving carbon to carbon unsaturated bonds, e.g. obtained by polycondensation
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28016Particle form
    • B01J20/28019Spherical, ellipsoidal or cylindrical
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/28Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties
    • B01J20/28014Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof characterised by their form or physical properties characterised by their form
    • B01J20/28033Membrane, sheet, cloth, pad, lamellar or mat
    • B01J20/28035Membrane, sheet, cloth, pad, lamellar or mat with more than one layer, e.g. laminates, separated sheets
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282Porous sorbents
    • B01J20/285Porous sorbents based on polymers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00Aspects relating to sorbent materials
    • B01J2220/50Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/54Sorbents specially adapted for analytical or investigative chromatography
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS, COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2220/00Aspects relating to sorbent materials
    • B01J2220/50Aspects relating to the use of sorbent or filter aid materials
    • B01J2220/58Use in a single column

Abstract

The invention concerns a size-exclusion filtering device (1) for filtering liquid samples, said device consisting of at least a filtering material (2) comprising at least a generally crosslinked organic polymer having gel filtration properties and in the form of porous particles, in particular beads. Said device is characterized in that the particles of the filtering material are dehydrated particles having been previously swollen by contacting the filtering material (2) with a preferably polar organic or aqueous solvent and then dehydrated.

Description

size exclusion filtration device, its manufacturing method and the filtration process resulting

The present invention relates to a size exclusion filtration device for filtering liquid samples, a method for manufacturing such a device and a size exclusion filtration method for the implementation of a device of the abovementioned type.

There are many methods for separating molecules of a sample today. One of these methods of particle separation is called chro atographie permeation or size exclusion chromatography or gel filtration or gel filtration or molecular sieving. To allow the implementation of such a method of separation, many manufacturers have developed filter matrices gel said gel filtration matrices.

Gel filtration separates molecules according to their size, more specifically their Stokes radius. The gel filtration matrices are formed from resin. The resin of such gels is composed of particles, in particular, highly porous microspheres. The pore diameter of a given resin is variable within predetermined limits. Therefore, when contacting a liquid sample with a resin, some sample molecules are too large to enter any ball and can not disseminate and outside in the liquid surrounding them. They therefore take some time to elute from the gel. Rather, certain other molecules are small enough to diffuse into all the balls in addition to the outdoor space. They will be delayed and will take much longer to get out of the gel. Some other intermediate sized molecules can enter some pores some balls. So they circulate in a certain proportion of the balls, in addition to the external space. More will be smaller, this proportion will be. They will then take some time to get out of the gel. This time will be proportional to the Stokes radius thus indirectly to their size so their mass.

A given gel has a specific fractionation range (expressed in terms of molecular weight) within which the molecules will take out the gel, a time proportional to their size, so their weight. Within this field, the molecules may be separated from each other. The molecules larger than the upper limit of fractionation, the exclusion limit, so go out together quite quickly. Molecules smaller than the lower limit out together much later. In both cases, it is obviously impossible to separate molecules according to their mass. The molecules having a mass between these two limits may be separated from each other. These limits are called fractionation range.

There are now on the market many different filter materials on gel stability, resistance to pressure and flow. The best known are today particularly manufactured by Pharmacia Company under the tradenames Sephadex or Sepharose by Bio-Rad Company under the trademark Biogel or by PIERCE Company under the trademark Fractogel.

In summary and as explained above, in the filtration techniques pat gel, the molecules of dimension greater than the largest pores of the particles of the filter material, that is to say above the upper limit of the fractionation material can not penetrate into the pores of the particles are first eluted and because they pass through the filter material in the liquid phase outside the particles. Smaller molecules can penetrate the pores of the particles based on their size and shape and are eluted more slowly. These gel filtration resins are used today all based on the same principle. Indeed, these resins are usually sold in powder form. They need for their job preparation being rehydrated using a suitable solvent, usually advised by the manufacturer of the resin. During this contacting step with an aqueous or polar organic solvent, preferably, the microporous particles of the filter material hydrate, swell. It is then possible to sample filtration. In this document, by hydrate is meant any molecule used in the composition of a polar organic or aqueous solvent, preferably, and the term hydrated material, any material containing hydrates. Such an example of preparation of gel filtration resins in particular is provided by the TJS-A-5,976,389 and Patent WO 97/48422. This contacting step the material with a polar solvent or aqueous origin and particle swelling may take several hours delaying analysis.

In addition, it is sometimes necessary, after this contacting step of the material with a polar solvent or aqueous origin, to remove a portion of the interstitial fluid introduced in excess into the filter material during the contacting the material with a polar solvent or aqueous origin. Such is the case of multiple processor samples described in international patent application WO 01/21311 where a centrifugation step is necessary to remove excess tissue fluid. This first use of the filter material resins thus requires always a step of contacting the material with a polar solvent or aqueous origin view of a swelling of the particles of the filter material, the sample is then contacted with the material hydrated filter.

These gel filtration resins can still be used as a water absorbent material as is illustrated in international application WO 99/67277. In this case, these resins are directly in contact with the sample which causes, during said contacting with said resin, a swelling constituent beads of the resin. The gel is then subsequently removed from contact with the sample and the sample may then be treated. In this case, the gel filtration resin is not used as a filter element but as the water-absorbent element.

In summary, the prior art clearly shows that the gel filtration resins are still used in the hydrated state as filter element or dehydrated state as water absorption element, the contacting step of the material with a polar or aqueous solvent while having origin intended to allow the swelling of absorbent material.

All these techniques do not allow the implementation of a gel filtration resin with an analysis device in which reagents have been incorporated in dry form, the presence of moisture in the filter material being capable of reacting the reagents of the assay device.

The inventors of the present invention have however found that, surprisingly, filtration gel resins used so far retained a number of their filtration properties by size exclusion when were subjected to contacting with a polar or aqueous solvent originally intended to cause swelling of the particles of the filter material and then a dehydration step to obtain a moisture-free filter material or substantially free of moisture and having a moisture content close to zero.

An object of the present invention is therefore to propose a filtration device size exclusion of a new type, the design of the filter material allows the production of a filter device ready for use directly usable by contacting with the sample to be filtered without special prior step of preparing the filter material, in particular by contacting said material with a polar solvent or aqueous origin.

Another object of the present invention is to provide a filtration device size exclusion of a new type, the design permits association with an analyzing device wherein the reagents are incorporated in dry form without generating deterioration or a beginning of the reaction of said reactants.

Another object of the present invention is to provide a method of manufacturing a filtration device of the aforementioned type easy to implement.

To this end, the inventipn is to obj and a size exclusion filtration device for filtering liquid samples, this device comprising at least a filter material comprising at least one generally cross-linked organic polymer having properties of a filtration medium gel and in the form of particles, especially beads, porous, characterized in that the particles of the filter material are particles having been previously swollen by contact with a polar organic any solvent or aqueous material of the filter then dried.

The presentation of the filtering material in dried form allows its direct use as a filter without requiring cumbersome steps of preparing said filter.

The invention further relates to a method for manufacturing a filtration device of the aforementioned type, the filter material of the device comprising a generally cross-linked organic polymer having properties of a gel filtration medium and in the form of particles such as porous microbeads, characterized in that hydrate the filter material by means of a polar organic or aqueous solvent to cause swelling of the particles of the material and in that dehydrates the filter material so as to form a filter material in dry form ready for use.

The invention will be better understood on reading the following description of exemplary embodiments with reference to the accompanying drawings in which: Figure 1 shows a schematic view of a filtration device according to the invention and

2 shows a schematic view of an embodiment of a filtration device associated with an evaluation device, particularly to an immunochromatographic assay device.

As mentioned above, the device 1 of filtration, object of the invention uses the principles of size exclusion. It consists for this purpose with 2 filter material comprising at least one generally cross-linked organic polymer having properties of a gel filtration medium and in the form of particles, especially beads, porous. The principle of the invention therefore consists in using the dry form a filtering material used usually in an aqueous medium. For this purpose, the particles of the filter material 2 are dry particles having been previously swollen by contacting with a solvent or aqueous organic polar preferably the filter material. As mentioned above, such filter materials have the ability to retain small particle size of a sample while allowing the large molecules. The material 2 base filter used in the filtration device 1 after contacting with a polar or aqueous organic solvent and preferably dehydration may be constituted by a gel filtration resin consisting of a polymer or dextran derivative of dextran whose fibers are crosslinked, the crosslinking agent preferably being consisting of epichlorohydrin or a derivative thereof. Such a base material to be subjected to a contacting step with an aqueous or polar organic solvent, preferably followed by a dehydration step, is for example sold by Pharmacia under the trademark Sephadex. Depending on the characteristics of the filter material, in particular based on its fractionation range, different references are proposed.

This material 2 base filter can also be constituted of at least one polymer acryla ide or a crosslinked acrylamide derivative, the crosslinking agent preferably consisting of bis-acrylamide or a derivative thereof -this. Such a product is sold especially by the BIO-RAD Company under the trademark Biogel. The filter material may also be constituted by a composite mixture of dextran and acrylamide or a composite mixture of derivatives thereof. Thus, by way of example, it may be cited the filter material sold under the trademark Sephacryl consisting of a cross-linked copolymer of allyl dextran and N, N 'methylene bis-acrylamide. The filter material may also be made of an agarose gel in preparation microbeads. Such a filtering material is especially sold under the trademark Sepharose by Pharmacia Corporation. Finally, such a filter material can also consist of at least one vinyl polymer, as illustrated by the product fractogène TSK (registered trademark) sold by PIERCE Company. Of course, other types of filter materials having the properties of a gel filtration medium can be used in equivalent way in the context of the present invention.

2 the filter material consisting of a of the aforementioned type gel filtration resin is subjected initially to a hydration step to cause swelling of the particles contained in said resin.

This hydration is usually carried out by contacting the filter material with a polar or aqueous organic solvent. Generally, the aqueous or polar organic solvent used has been specified by the manufacturer of the gel filtration resin. Examples include the following solvents:

• Tris EDTA solvent:

Tris-HCl pH = 8, 0 10 mM EDTA pH = 8 0, 1 mM EDTA or Tris acetate solvent

40 mM Tris

Sodium acetate 5mM

1mM EDTA or

. solvent (Tris EDTA NaCl)

Tris-HCl pH 8.0 40 mM

NaCl 5 mM

1mM EDTA or

. solvent (Tris Borate EDTA)

89 mM Tris

89 mM boric acid

2.5 M EDTA

Once this step of contacting with a polar or aqueous organic solvent preferably operated, dried filter material. Dehydration is preferably carried out by evaporating the solvent. Once performed dehydration step, the particles of the filter material 2, which are in the dehydrated state, constitute a filtering material ready for use, in particular by direct contacting with the sample to be filtered.

In a preferred embodiment of the filter device, the filter material 2 is sandwiched between two supports 3 shots, preferably porous. At least one of the supports 3 is preferably formed of a sheet of a porous material, such as a nitrocellulose sheet. Thus, before or after a contacting step with an aqueous or polar organic solvent preferably from the filter material, the filter material is arranged between two flat substrates, preferably porous, and the assembly is subjected to a complex of the obtained dehydration step for obtaining a filtration device ready for use. Preferably, the complex formed of the filtering material and preferably porous media is disposed within a mold cavity prior to dehydration.

The manufacture of such a filtration device can thus be carried out as follows. A composition of Sephadex (trademark) rehydrated according to the manufacturer's instructions in a suitable buffer and optionally comprising other different products such as cellulose, is deposited on a sheet 3 placed on the bottom of a cup of the same size as the sheet and the second sheet 3 is placed on the rehydrated composition. The assembly is placed at 37 ° C for sufficient time for its complete dehydration optionally in a dry atmosphere and / or ventilated. After complete dehydration, the filter is removed from the mold and then possibly cut as required and depending on its future use.

The filtration device obtained can be used alone or in combination with an analysis device, such as a chemical analysis device, physical, physicochemical or immunological. 2 illustrates the use of this filter device upstream of an immunochromatographic assay device. This immuno-chromatographic test device is constituted by at least a first support 4 on which was deposited a ligand coupled to an indicator ,, a second support 5 optionally common to the first support 4 on which is fixed at least one reagent and an absorbent 6, filter 1, supports 4, 5 and 6 being disposed absorbent online optionally partially overlapping and joined to each other to form a test strip. The assembly is here obtained by means of an adhesive shown at 7 in Figure 2.

Such a device is applicable to any analysis using an affinity reaction or not characterized by the presence of a reagent fixed to a support which may be, without limitation, a membrane and the use of a staining reagent. The developing reagent may include particulate components giving a coloration or reactive molecules, such as enzymes, the reaction yielded a coloration or no coloration indicative of the nature of the result. The element sought by such an analysis device may be biological, an antibody or antigen, chemical or biochemical nature.

Such a device is also applicable when the desired element is one or more antigens specific for one or more pathologies. Such an analysis device may also be applicable in the therapeutic monitoring framework. Thus, by way of example, the filter device can be associated with an immunochromatographic assay device in which an antibody having an affinity for fluorescein is attached to a nitrocellulose membrane (eg. Support 5), and wherein streptavidin coupled to colloidal gold is present in free form (eg. carrier 4). The procedure, on the basis of a sample containing DNA, a PCR using a first primer labeled bifluorescéine and a second primer labeled with biotin. At the end of amplification, if the primers have not been incorporated into an amplicon (amplification product), the primers are labeled with biotin capture streptavidin coupled to colloidal gold but will not be captured by the anti-fluorescein antibody bound. Only primers labeled with fluorescein will be captured and can not produce a color indication. The absence of a signal will indicate much negative amplification result. In the case of a positive amplification, the primers are incorporated into the amplicons. Each of them thus has a primer labeled with fluorescein and a biotin labeled primer. When the sample passes through the filtration device described above, remained free primers are selected so that the amplicons to diffuse coupled analysis device. The amplicons are then capture streptavidin coupled to colloidal gold can be captured by anti-fluorescein antibody attached to the carrier forming membrane 5. A red-violet color signal appears indicating the positive result of the amplification.

The filter device can, similarly, be in the form of a column or a tubular device. In this case, the filter material is housed within said tubular body and may be retained within the body by filtration membranes known per se.

The filtration device 1 described above allows the implementation of a filtration process by size exclusion liquid sample wherein the sample is filtered by direct contacting of this sample with the dried filter material filtration device. This results in significant time savings for the realization of analysis. As mentioned above, the applications of this filtering device are numerous. For example, a first application to the detection of amplification product was cited above. Other examples can also be described. Thus, it is necessary, in some cases, to detect the free form of a pharmaceutical product present for example in the blood of a patient. These include all molecules in cancer therapeutic that will have a toxic effect on cancer cells when combined with a carrier protein molecule in the blood. Indeed, some of these molecules can have toxic side effects in the free form body.

It will then be necessary to isolate the free molecules of bound molecules to detect only free. The filtration device associated with one of these molecules detection device contacted with a blood sample, serum or plasma, will allow to detect only the free molecules. The patient's therapeutic monitoring will then be optimized by administering treatment material as soon as the rate of free molecules is less than the toxicity threshold.

Another example of application is the detection of biological markers. This may be the case in environment or food, but also in the field of health.

For prostate cancer screening, the marker is used as the PSA, prostate specific antigen. Whereas before, we used only the total PSA levels in urine or blood for laboratory diagnosis, it is now recognized that greater specificity and greater indicative and predictive value in determining is obtained the free PSA and that of PSA bound to α-chymotrypsin. Most methods carry out the determination of the two rates by removing the other levels of total PSA. The filtration device of the present invention, associated with a conventional detection of total PSA system would determine the PSA bound by elimination of free PSA.

The same system without the filtration system determining the total PSA, we obtain the PSA bound by subtraction.

Claims

1. Device (1) filtration, size exclusion for filtration of liquid samples, said device being constituted at least of a material (2) filter comprising at least one generally cross-linked organic polymer having properties of a medium gel filtration and in the form of particles, especially beads, porous, characterized in that the particles of the filter material are particles having been previously swollen by contacting with a polar or aqueous organic preferably solvent material ( 2) filtering and then dried.
2. Device (1) according to claim 1, characterized in that the particles of the material (2) filter are, in the dehydrated state, ready for use by including in direct contact with the sample to be filtered.
3. Device according to one of Claims 1 and 2, characterized in that the material (2) filter is sandwiched between two supports (3) planes, preferably porous.
4. Device according to claim 3, characterized in that at least one of the supports (3) consists of a sheet of porous material, such as a nitrocellulose sheet.
5. Device according to one of Claims 1 and 2, characterized in that the material (2) filter is housed inside a tubular body such as a column.
6. Filter device according to one of claims 1 to 5, characterized in that it is arranged to be associated with an analysis device, such as a chemical analysis device, physical, physicochemical or immunological .
7. Filtration device according to one of claims 1 to 6, characterized in that it is arranged to be disposed upstream of an immunochromatographic assay device consists of at least a first support (4) which was deposited a ligand coupled to an indicator, a second support (5) optionally common to the first support (4) on which is fixed at least one reagent and an absorbent (6), means (1) filtration , supports (4, 5) and absorbent (6) being arranged in line and assembled optionally part overlap with each other to form a test strip.
8. Device according to one of claims 1 to 7, characterized in that the filter material is a gel filtration resin consisting of a polymer of dextran or dextran derivative in which the fibers are crosslinked, the crosslinking agent being preferably constituted by one epichlorohydrin or a derivative thereof
9. Device according to one of claims 1 to 7, characterized in that the filter material consists at least of an acrylamide polymer or a crosslinked acrylamide derivative, the crosslinking agent preferably consisting bis-acrylamide or a derivative thereof.
10. Device according to one of claims 1 to 7, characterized in that the filter material consists of a composite mixture of dextran and acrylamide or a composite mixture of derivatives thereof.
11. Device according to one of claims 1 to 7, characterized in that the filter material consists of a preparation of agarose gel microbeads.
12. Device according to one of claims 1 to 7, characterized in that the filter material consists of at least one vinyl polymer.
13. A method of manufacturing a device (1) including filtration accordance with one of claims 1 to 12, the material (2) of the filter device comprising a generally cross-linked organic polymer having properties of a filtration medium gel and in the form of particles such as porous microbeads, characterized in that hydrate the material (2) filter by contact with a polar organic or aqueous solvent, preferably in order to cause swelling of the particles of the material and in that the dried material (2) filtering to form a filter material in dry form ready for use.
14. The manufacturing method according to claim 13, characterized in that the dried material (2) filter by evaporating the solvent.
15. Manufacturing process according to one of Claims 12 and 13, characterized in that, before or after a contacting step with an aqueous or polar organic solvent preferably from the material (2) filter, there is the material filter between two supports (3) planes, preferably porous, and subjecting the whole of the complex obtained to a drying step for obtaining a filtering device ready for use.
16. The manufacturing method according to claim 15, characterized in that the complex formed of the filtering material and preferably porous media is disposed within a mold cavity prior to dehydration.
17. Production method according to one of claims 12 to 16, characterized in that the filter material is according to any one of claims 1 to 12.
18. A method of filtration size exclusion of a liquid sample by means of a filtering device according to any one of claims 1 to 12, characterized in that the filter by direct contacting of the sample with the dried filter material.
PCT/FR2002/003990 2001-11-21 2002-11-18 Size-exclusion filtering device, method for making same and resulting filtering method WO2003043713A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR01/15032 2001-11-21
FR0115032A FR2832321B1 (en) 2001-11-21 2001-11-21 size exclusion filtration device, its manufacturing process and the filtration process resulting in

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20037016793A KR20040067878A (en) 2001-11-21 2002-11-18 Size-exclusion filtering device, method for making same and resulting filtering method
EP20020803454 EP1446211A1 (en) 2001-11-21 2002-11-18 Size-exclusion filtering device, method for making same and resulting filtering method
JP2003545388A JP2005509866A (en) 2001-11-21 2002-11-18 Size exclusion filtration device, manufacturing method, and, the filtration process
CA 2451561 CA2451561A1 (en) 2001-11-21 2002-11-18 Size-exclusion filtering device, method for making same and resulting filtering method

Publications (1)

Publication Number Publication Date
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Country Status (6)

Country Link
EP (1) EP1446211A1 (en)
JP (1) JP2005509866A (en)
KR (1) KR20040067878A (en)
CA (1) CA2451561A1 (en)
FR (1) FR2832321B1 (en)
WO (1) WO2003043713A1 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3002823A (en) * 1958-04-16 1961-10-03 Pharmacia Ab Process of separating materials having different molecular weights and dimensions
FR2484858A1 (en) * 1980-04-21 1981-12-24 Hitachi Chemical Co Ltd Before-and column column cartridge for liquid chromatography
DE3217032A1 (en) * 1982-03-26 1983-10-06 Armin Dipl Chem Dr Rer N Gilak Simplified separation method for immunological determinations, dry chromatographic column for carrying out the method, and kit containing this
EP0143412A2 (en) * 1983-11-25 1985-06-05 Boehringer Mannheim Gmbh Immunochemical quick-test
GB2220867A (en) * 1988-06-22 1990-01-24 Indforschzentrum Biotech Veb Device and method for chromatographic separation
WO2001021311A1 (en) * 1999-09-20 2001-03-29 Princeton Separations Device for multiple sample processing

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3002823A (en) * 1958-04-16 1961-10-03 Pharmacia Ab Process of separating materials having different molecular weights and dimensions
FR2484858A1 (en) * 1980-04-21 1981-12-24 Hitachi Chemical Co Ltd Before-and column column cartridge for liquid chromatography
DE3217032A1 (en) * 1982-03-26 1983-10-06 Armin Dipl Chem Dr Rer N Gilak Simplified separation method for immunological determinations, dry chromatographic column for carrying out the method, and kit containing this
EP0143412A2 (en) * 1983-11-25 1985-06-05 Boehringer Mannheim Gmbh Immunochemical quick-test
GB2220867A (en) * 1988-06-22 1990-01-24 Indforschzentrum Biotech Veb Device and method for chromatographic separation
WO2001021311A1 (en) * 1999-09-20 2001-03-29 Princeton Separations Device for multiple sample processing

Also Published As

Publication number Publication date Type
EP1446211A1 (en) 2004-08-18 application
FR2832321A1 (en) 2003-05-23 application
JP2005509866A (en) 2005-04-14 application
FR2832321B1 (en) 2004-01-09 grant
CA2451561A1 (en) 2003-05-30 application
KR20040067878A (en) 2004-07-30 application

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