WO2003027136A3 - Protein production - Google Patents

Protein production Download PDF

Info

Publication number
WO2003027136A3
WO2003027136A3 PCT/GB2002/004223 GB0204223W WO03027136A3 WO 2003027136 A3 WO2003027136 A3 WO 2003027136A3 GB 0204223 W GB0204223 W GB 0204223W WO 03027136 A3 WO03027136 A3 WO 03027136A3
Authority
WO
WIPO (PCT)
Prior art keywords
proteins
protein
mixer
dilution
inclusion bodies
Prior art date
Application number
PCT/GB2002/004223
Other languages
French (fr)
Other versions
WO2003027136A2 (en
Inventor
Edward Fahey
James Alexander Purvis
John William Stairmand
Original Assignee
Accentus Plc
Edward Fahey
James Alexander Purvis
John William Stairmand
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Accentus Plc, Edward Fahey, James Alexander Purvis, John William Stairmand filed Critical Accentus Plc
Priority to AU2002331948A priority Critical patent/AU2002331948A1/en
Priority to EP02767646A priority patent/EP1432504A2/en
Priority to GBGB0229967.5A priority patent/GB0229967D0/en
Publication of WO2003027136A2 publication Critical patent/WO2003027136A2/en
Publication of WO2003027136A3 publication Critical patent/WO2003027136A3/en
Priority to EP03797361A priority patent/EP1551454A1/en
Priority to AU2003253000A priority patent/AU2003253000A1/en
Priority to PCT/GB2003/003550 priority patent/WO2004026340A1/en
Priority to CA002499398A priority patent/CA2499398A1/en
Priority to CNA038221446A priority patent/CN1697660A/en
Priority to US10/528,406 priority patent/US7183378B2/en
Priority to JP2004537252A priority patent/JP2006511472A/en
Priority to NO20051466A priority patent/NO20051466L/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
    • C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F2025/91—Direction of flow or arrangement of feed and discharge openings
    • B01F2025/913—Vortex flow, i.e. flow spiraling in a tangential direction and moving in an axial direction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2215/00—Auxiliary or complementary information in relation with mixing
    • B01F2215/04—Technical information in relation with mixing
    • B01F2215/0413—Numerical information
    • B01F2215/0436—Operational information
    • B01F2215/0477—Numerical time values
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2208/00—Processes carried out in the presence of solid particles; Reactors therefor
    • B01J2208/00008—Controlling the process
    • B01J2208/00548—Flow
    • B01J2208/00557—Flow controlling the residence time inside the reactor vessel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Proteins may be made by genetically engineered microorganisms, the protein being stored in the form of inclusion bodies (IB). The proteins in the inclusion bodies are in an insoluble and inactive form. They may be dissolved using a solubilisation reagent (18), and the resulting solution diluted so that the proteins refold into the active form. The dilution is performed by passing the solution and a diluting liquid into a fluidic vortex mixer (20), the dimensions of the mixer and the flow rates being such that the residence time of the liquid in the mixer (20) is less than 1 second. The very efficient mixing reduces the proportion of the protein that re-aggregates, and reduces the required degree of dilution so as to increase the concentration of the active, correctly-folded protein.
PCT/GB2002/004223 2001-09-26 2002-09-18 Protein production WO2003027136A2 (en)

Priority Applications (11)

Application Number Priority Date Filing Date Title
AU2002331948A AU2002331948A1 (en) 2001-09-26 2002-09-18 Protein production
EP02767646A EP1432504A2 (en) 2001-09-26 2002-09-18 Protein production
GBGB0229967.5A GB0229967D0 (en) 2002-09-18 2002-12-21 Protein production
JP2004537252A JP2006511472A (en) 2002-09-18 2003-08-14 Protein production
US10/528,406 US7183378B2 (en) 2002-09-18 2003-08-14 Protein production
EP03797361A EP1551454A1 (en) 2002-09-18 2003-08-14 Protein production
AU2003253000A AU2003253000A1 (en) 2002-09-18 2003-08-14 Protein production
PCT/GB2003/003550 WO2004026340A1 (en) 2002-09-18 2003-08-14 Protein production
CA002499398A CA2499398A1 (en) 2002-09-18 2003-08-14 Protein production
CNA038221446A CN1697660A (en) 2002-09-18 2003-08-14 Protein production
NO20051466A NO20051466L (en) 2002-09-18 2005-03-18 Protein Production.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0123114.1A GB0123114D0 (en) 2001-09-26 2001-09-26 Protein production
GB0123114.1 2001-09-26

Publications (2)

Publication Number Publication Date
WO2003027136A2 WO2003027136A2 (en) 2003-04-03
WO2003027136A3 true WO2003027136A3 (en) 2003-06-19

Family

ID=9922707

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/004223 WO2003027136A2 (en) 2001-09-26 2002-09-18 Protein production

Country Status (4)

Country Link
EP (1) EP1432504A2 (en)
AU (1) AU2002331948A1 (en)
GB (1) GB0123114D0 (en)
WO (1) WO2003027136A2 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BRPI0614440A2 (en) * 2005-07-29 2011-03-29 Novartis Ag in vitro protein folding method and system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5921678A (en) * 1997-02-05 1999-07-13 California Institute Of Technology Microfluidic sub-millisecond mixers
EP1123734A2 (en) * 2000-02-03 2001-08-16 Cellular Process Chemistry Inc. Miniaturized reaction apparatus
WO2001064332A1 (en) * 2000-03-02 2001-09-07 Newcastle Universtiy Ventures Limited Capillary reactor distribution device and method
WO2001089675A2 (en) * 2000-05-24 2001-11-29 Micronics, Inc. Jet vortex mixer

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5921678A (en) * 1997-02-05 1999-07-13 California Institute Of Technology Microfluidic sub-millisecond mixers
EP1123734A2 (en) * 2000-02-03 2001-08-16 Cellular Process Chemistry Inc. Miniaturized reaction apparatus
WO2001064332A1 (en) * 2000-03-02 2001-09-07 Newcastle Universtiy Ventures Limited Capillary reactor distribution device and method
WO2001089675A2 (en) * 2000-05-24 2001-11-29 Micronics, Inc. Jet vortex mixer

Also Published As

Publication number Publication date
WO2003027136A2 (en) 2003-04-03
AU2002331948A1 (en) 2003-04-07
EP1432504A2 (en) 2004-06-30
GB0123114D0 (en) 2001-11-14

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