WO2003008436A3 - Methods for the identification of peptidyl compounds interacting with extracellular target molecules - Google Patents

Methods for the identification of peptidyl compounds interacting with extracellular target molecules Download PDF

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Publication number
WO2003008436A3
WO2003008436A3 PCT/US2002/023375 US0223375W WO03008436A3 WO 2003008436 A3 WO2003008436 A3 WO 2003008436A3 US 0223375 W US0223375 W US 0223375W WO 03008436 A3 WO03008436 A3 WO 03008436A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
target molecules
identification
extracellular target
peptidyl compounds
Prior art date
Application number
PCT/US2002/023375
Other languages
French (fr)
Other versions
WO2003008436A2 (en
Inventor
Frederick S Hagen
Richard G Woodbury
Pieter J Oort
Original Assignee
Icogen Corp
Frederick S Hagen
Richard G Woodbury
Pieter J Oort
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Icogen Corp, Frederick S Hagen, Richard G Woodbury, Pieter J Oort filed Critical Icogen Corp
Priority to AU2002326437A priority Critical patent/AU2002326437A1/en
Priority to US10/484,271 priority patent/US20050255464A1/en
Publication of WO2003008436A2 publication Critical patent/WO2003008436A2/en
Publication of WO2003008436A3 publication Critical patent/WO2003008436A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells

Abstract

The present invention provides libraries expressing peptide libraries on the extracellular cell surface of host cells and methods for identifying peptides that bind extracellular target molecules under the physiological conditions encountered in biological fluids and secretions. The present invention is also directed to vectors for expressing gene fusion proteins and for targeting those fusion proteins to the extracellular cell surface.
PCT/US2002/023375 2001-07-19 2002-07-18 Methods for the identification of peptidyl compounds interacting with extracellular target molecules WO2003008436A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2002326437A AU2002326437A1 (en) 2001-07-19 2002-07-18 Methods for the identification of peptidyl compounds interacting with extracellular target molecules
US10/484,271 US20050255464A1 (en) 2001-07-19 2002-07-18 Methods for the identification of peptidyl compounds interacting with extracellular target molecules

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US30692401P 2001-07-19 2001-07-19
US60/306,924 2001-07-19

Publications (2)

Publication Number Publication Date
WO2003008436A2 WO2003008436A2 (en) 2003-01-30
WO2003008436A3 true WO2003008436A3 (en) 2004-09-16

Family

ID=23187480

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2002/023375 WO2003008436A2 (en) 2001-07-19 2002-07-18 Methods for the identification of peptidyl compounds interacting with extracellular target molecules

Country Status (2)

Country Link
AU (1) AU2002326437A1 (en)
WO (1) WO2003008436A2 (en)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999041368A2 (en) * 1998-02-11 1999-08-19 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999041368A2 (en) * 1998-02-11 1999-08-19 Maxygen, Inc. Optimization of immunomodulatory properties of genetic vaccines

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
CHANG C.J.: "Evolution of a cytokine using DNA family shuffling", NATURE BIOTECHNOLOGY, vol. 17, 7 August 1999 (1999-08-07), pages 793 - 797, XP002165178 *
COURVALIN P.: "Gene transfer from bacteria to mammalian cells", C.R. ACAD. SCI. PARIS, SCIENCES DE LA VIE/LIFE SCIENCES, vol. 318, 1995, pages 1207 - 1212, XP000579701 *
DOWER W.J.: "Targeting growth factor and cytokine receptors with recombinant peptide libraries", CURRENT OPINION IN CHEMICAL BIOLOGY, vol. 2, 1998, pages 328 - 334, XP002978616 *
LOWMAN H.B.: "Monovalent phage display: A method for selecting variant proteins from random libraries", METHODS: A COMPANION TO METHODS IN ENZYMOLOGY, vol. 3, no. 3, December 1991 (1991-12-01), pages 205 - 216, XP002978695 *
YANOFSKY S.D.: "High affinity type I interleukin 1 receptor antagonists discovered by screening recombinant peptide libraries", PNAS USA, vol. 93, July 1996 (1996-07-01), pages 7381 - 7386, XP000985913 *

Also Published As

Publication number Publication date
AU2002326437A1 (en) 2003-03-03
WO2003008436A2 (en) 2003-01-30

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