WO2002102970A3 - Flax (linum usitatissimim l.) seed-specific promoters - Google Patents

Flax (linum usitatissimim l.) seed-specific promoters Download PDF

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Publication number
WO2002102970A3
WO2002102970A3 PCT/IB2002/003184 IB0203184W WO02102970A3 WO 2002102970 A3 WO2002102970 A3 WO 2002102970A3 IB 0203184 W IB0203184 W IB 0203184W WO 02102970 A3 WO02102970 A3 WO 02102970A3
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WO
WIPO (PCT)
Prior art keywords
flax
seed
promoters
usitatissimim
linum
Prior art date
Application number
PCT/IB2002/003184
Other languages
French (fr)
Other versions
WO2002102970A2 (en
Inventor
Xiao Qiu
Martin Truksa
Zhiyuan Hu
Original Assignee
Bioriginal Food & Science Corp
Xiao Qiu
Martin Truksa
Zhiyuan Hu
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bioriginal Food & Science Corp, Xiao Qiu, Martin Truksa, Zhiyuan Hu filed Critical Bioriginal Food & Science Corp
Priority to DE60228272T priority Critical patent/DE60228272D1/en
Priority to EP02765152A priority patent/EP1423521B1/en
Priority to AU2002329522A priority patent/AU2002329522B2/en
Priority to CA2448501A priority patent/CA2448501C/en
Publication of WO2002102970A2 publication Critical patent/WO2002102970A2/en
Publication of WO2002102970A3 publication Critical patent/WO2002102970A3/en
Priority to AU2008203820A priority patent/AU2008203820B8/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pregnancy & Childbirth (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Reproductive Health (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Nutrition Science (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

The present invention is directed to promoters of flax conlinin and ro-3 desaturase genes. The promoters guide high levels of the expression exclusively in flax developing seeds. This specific expression pattern concomitant with the biosynthesis of storage lipids and proteins make these promoters particularly useful for seed-specific modification of fatty acid and protein compositions in plant seeds.
PCT/IB2002/003184 2001-06-06 2002-06-06 Flax (linum usitatissimim l.) seed-specific promoters WO2002102970A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
DE60228272T DE60228272D1 (en) 2001-06-06 2002-06-06 SEED-SPECIFIC SEQUENCES OF FLAX (LINUM USITATISSIMUM L.)
EP02765152A EP1423521B1 (en) 2001-06-06 2002-06-06 Flax (linum usitatissimum l.) seed-specific sequences
AU2002329522A AU2002329522B2 (en) 2001-06-06 2002-06-06 Flax (Linum usitatissimim L.) seed-specific promoters
CA2448501A CA2448501C (en) 2001-06-06 2002-06-06 Flax (linum usitatissimum l.) seed-specific promoters
AU2008203820A AU2008203820B8 (en) 2001-06-06 2008-08-12 Flax (Linum unitatissimum L.) seed-specific promoters

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US29582301P 2001-06-06 2001-06-06
US60/295,823 2001-06-06

Publications (2)

Publication Number Publication Date
WO2002102970A2 WO2002102970A2 (en) 2002-12-27
WO2002102970A3 true WO2002102970A3 (en) 2004-03-18

Family

ID=23139369

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IB2002/003184 WO2002102970A2 (en) 2001-06-06 2002-06-06 Flax (linum usitatissimim l.) seed-specific promoters

Country Status (8)

Country Link
US (3) US7193134B2 (en)
EP (2) EP2184359B1 (en)
AT (1) ATE404672T1 (en)
AU (2) AU2002329522B2 (en)
CA (2) CA2448501C (en)
DE (1) DE60228272D1 (en)
ES (1) ES2312620T3 (en)
WO (1) WO2002102970A2 (en)

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MXPA06009572A (en) 2004-02-27 2007-05-23 Basf Plant Science Gmbh Method for producing polyunsaturated fatty acids in transgenic plants.
BRPI0511236A (en) * 2004-06-04 2007-11-27 Fluxome Sciences As A method for producing four or more double bond polyunsaturated fatty acids, genetically modified saccharomyces cerevisiae which is capable of producing four or more double bond polyunsaturated fatty acids when developed on a substrate other than fatty acid, composition, use of the composition and use of a genetically modified saccharomyces cervisiae
AR052203A1 (en) 2005-02-09 2007-03-07 Bioriginal Food & Science Corp NEW MEMBERS OF THE OMEGA FAT ACID DESATURASA FAMILY - 3 AND USES OF THESE
US20090031440A1 (en) * 2005-02-26 2009-01-29 Basf Plant Science Gmbh Expression Cassettes for Seed-Preferential Expression in Plants
WO2006111541A2 (en) * 2005-04-20 2006-10-26 Basf Plant Science Gmbh Expression cassettes for seed-preferential expression in plants
US7923598B2 (en) * 2006-06-22 2011-04-12 Bioriginal Food & Science Corp. Fatty acid hydroxylases and uses thereof
EP2222856A2 (en) 2007-12-14 2010-09-01 BASF Plant Science GmbH Promoters from brassica napus for seed specific gene expression
EP2285967B1 (en) 2008-06-03 2016-12-21 BASF Plant Science GmbH Fatty acid dehydratases and uses thereof
CA2729496C (en) 2008-07-01 2018-02-13 Basf Plant Science Gmbh Promoters from brassica napus for seed specific gene expression
ES2644883T3 (en) 2008-11-18 2017-11-30 Commonwealth Scientific And Industrial Research Organisation Enzymes and methods to produce omega-3 fatty acids
US10519458B2 (en) 2009-04-22 2019-12-31 Basf Plant Science Company Gmbh Whole seed specific promoter
EP2821492A3 (en) 2009-05-13 2015-04-08 BASF Plant Science Company GmbH Acyltransferases and uses thereof in fatty acid production
EP2440662B1 (en) 2009-06-08 2018-08-01 BASF Plant Science Company GmbH Novel fatty acid elongation components and uses thereof
EP2451958A1 (en) 2009-07-10 2012-05-16 BASF Plant Science Company GmbH Expression cassettes for endosperm-specific expression in plants
CA2768082C (en) 2009-07-17 2020-07-21 Basf Plant Science Company Gmbh Novel fatty acid desaturases and uses thereof
CN112011566B (en) 2009-08-31 2023-12-05 巴斯夫植物科学有限公司 Regulatory nucleic acid molecules for enhancing seed-specific gene expression in plants to promote enhanced polyunsaturated fatty acid synthesis
EP2504427B1 (en) 2009-11-24 2018-06-27 BASF Plant Science Company GmbH Novel fatty acid desaturase and uses thereof
US9347049B2 (en) 2009-11-24 2016-05-24 Basf Plant Science Company Gmbh Fatty acid elongase and uses thereof
AU2010325720A1 (en) 2009-12-03 2012-07-19 Basf Plant Science Company Gmbh Expression cassettes for embryo-specific expression in plants
EP2585603B1 (en) 2010-06-25 2017-12-20 BASF Plant Science Company GmbH Acyltransferases and uses therof in fatty acid production
AR083323A1 (en) 2010-06-28 2013-02-21 Commw Scient Ind Res Org METHODS TO PRODUCE LIPIDS
DE112011103527T5 (en) 2010-10-21 2013-10-17 Basf Plant Science Company Gmbh New fatty acid desaturases, elongases, elongation components and applications thereof
EP2798065B1 (en) 2011-12-27 2019-06-05 Commonwealth Scientific and Industrial Research Organisation Simultaneous gene silencing and supressing gene silencing in the same cell
AU2012327162A1 (en) 2011-12-27 2013-07-11 Commonwealth Scientific And Industrial Research Organisation Production of dihydrosterculic acid and derivatives thereof
MY188956A (en) 2011-12-27 2022-01-14 Commw Scient Ind Res Org Processes for producing lipids
US8816111B2 (en) 2012-06-15 2014-08-26 Commonwealth Scientific And Industrial Research Organisation Lipid comprising polyunsaturated fatty acids
US9879234B2 (en) 2012-08-03 2018-01-30 Basf Plant Science Company Gmbh Enzymes, enzyme components and uses thereof
MX2016000225A (en) 2013-07-05 2016-06-15 Basf Plant Science Co Gmbh Gene expression or activity enhancing elements.
WO2015196250A1 (en) 2014-06-27 2015-12-30 Commonwealth Scientific And Industrial Research Organisation Lipid comprising docosapentaenoic acid
CN111154724B (en) 2013-12-18 2024-02-06 联邦科学技术研究组织 Extracted plant lipids comprising docosahexaenoic acid
ES2969618T3 (en) 2014-07-07 2024-05-21 Nuseed Global Innovation Ltd Processes to produce industrial plant lipid products
JP7423177B2 (en) 2014-11-14 2024-01-29 ビーエーエスエフ プラント サイエンス カンパニー ゲーエムベーハー Modification of vegetable lipids containing PUFA
SG11201804169QA (en) 2015-11-18 2018-06-28 Commw Scient Ind Res Org Rice grain with thickened aleurone
BR112018072473A2 (en) 2016-05-20 2019-02-19 Basf Agro Bv nucleic acid molecule, expression cassette, plant cell, plant, seed, methods for expressing a nucleic acid, producing a plant, controlling vegetation and cultivating the plant, useful combination for control, process for preparing a combination and using a combination
CA3054060C (en) 2017-02-22 2023-09-26 Yield10 Bioscience, Inc. Transgenic land plants comprising enhanced levels of mitochondrial transporter protein
WO2021183803A1 (en) 2020-03-11 2021-09-16 BASF Agricultural Solutions Seed US LLC Transcription regulating nucleotide sequences and methods of use
WO2024099765A2 (en) 2022-11-10 2024-05-16 BASF Agricultural Solutions Seed US LLC Transcription regulating nucleotide sequences and methods of use
WO2024107812A1 (en) 2022-11-15 2024-05-23 Basf Corporation Methods of reducing conidiation in cell culture

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WO1998018948A1 (en) * 1996-10-31 1998-05-07 National Research Council Of Canada Flax promoters for manipulating gene expression
WO2001016340A1 (en) * 1999-08-27 2001-03-08 Sembiosys Genetics Inc. Flax seed specific promoters

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WO1994018337A1 (en) * 1993-02-05 1994-08-18 Monsanto Company Altered linolenic and linoleic acid content in plants
WO1998018948A1 (en) * 1996-10-31 1998-05-07 National Research Council Of Canada Flax promoters for manipulating gene expression
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Also Published As

Publication number Publication date
WO2002102970A2 (en) 2002-12-27
CA2680376A1 (en) 2002-12-27
AU2008203820B2 (en) 2011-10-06
EP2184359A1 (en) 2010-05-12
CA2448501A1 (en) 2002-12-27
US7473820B2 (en) 2009-01-06
US20090172835A1 (en) 2009-07-02
CA2448501C (en) 2013-06-04
DE60228272D1 (en) 2008-09-25
ATE404672T1 (en) 2008-08-15
US20030159174A1 (en) 2003-08-21
EP1423521B1 (en) 2008-08-13
ES2312620T3 (en) 2009-03-01
AU2008203820B8 (en) 2012-02-02
AU2008203820A1 (en) 2008-09-04
US7193134B2 (en) 2007-03-20
AU2002329522B2 (en) 2008-05-22
EP2184359B1 (en) 2016-03-02
EP1423521A2 (en) 2004-06-02
US8841512B2 (en) 2014-09-23
US20070192902A1 (en) 2007-08-16
CA2680376C (en) 2016-11-15

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