WO2002083893A3 - Isolation of dna molecules using mercapto-aryl ligands - Google Patents

Isolation of dna molecules using mercapto-aryl ligands Download PDF

Info

Publication number
WO2002083893A3
WO2002083893A3 PCT/EP2002/004152 EP0204152W WO02083893A3 WO 2002083893 A3 WO2002083893 A3 WO 2002083893A3 EP 0204152 W EP0204152 W EP 0204152W WO 02083893 A3 WO02083893 A3 WO 02083893A3
Authority
WO
WIPO (PCT)
Prior art keywords
molecules
isolation
mercapto
dna molecules
aryl ligands
Prior art date
Application number
PCT/EP2002/004152
Other languages
French (fr)
Other versions
WO2002083893A2 (en
Inventor
Raf Lemmens
Tomas Nyhammar
Jan Berglof
Joachim Stadler
Original Assignee
Amersham Biosciences Ab
Raf Lemmens
Tomas Nyhammar
Jan Berglof
Joachim Stadler
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from SE0101380A external-priority patent/SE0101380D0/en
Priority claimed from SE0103436A external-priority patent/SE0103436D0/en
Application filed by Amersham Biosciences Ab, Raf Lemmens, Tomas Nyhammar, Jan Berglof, Joachim Stadler filed Critical Amersham Biosciences Ab
Priority to AU2002302534A priority Critical patent/AU2002302534B2/en
Priority to AT02730158T priority patent/ATE471980T1/en
Priority to EP02730158A priority patent/EP1379645B1/en
Priority to DE60236789T priority patent/DE60236789D1/en
Priority to JP2002582231A priority patent/JP4081376B2/en
Priority to US10/473,239 priority patent/US6916919B2/en
Priority to CA2444259A priority patent/CA2444259C/en
Publication of WO2002083893A2 publication Critical patent/WO2002083893A2/en
Publication of WO2002083893A3 publication Critical patent/WO2002083893A3/en

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/286—Phases chemically bonded to a substrate, e.g. to silica or to polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention relates to a process for separating nucleic acid molecules, preferably open circular and supercoiled plasmid DNA and RNA molecules from each other, comprising the steps of providing a solution comprising the molecules; adsorbing the molecules to adsorbing groups on a carrier; and optionally washing the column with a suitable solution. The present process is especially suitable for large-scale isolation of supercoiled ccc DNA to be used in gene therapy.
PCT/EP2002/004152 2001-04-18 2002-04-15 Isolation of dna molecules using mercapto-aryl ligands WO2002083893A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
AU2002302534A AU2002302534B2 (en) 2001-04-18 2002-04-15 Isolation of DNA molecules using mercapto-aryl ligands
AT02730158T ATE471980T1 (en) 2001-04-18 2002-04-15 ISOLATION OF DNA MOLECULES USING ARYLMERCAPTO LIGANDS
EP02730158A EP1379645B1 (en) 2001-04-18 2002-04-15 Isolation of dna molecules using mercapto-aryl ligands
DE60236789T DE60236789D1 (en) 2001-04-18 2002-04-15 ISOLATION OF DNA MOLECULES USING ARYLMERCAPTO LIGANDS
JP2002582231A JP4081376B2 (en) 2001-04-18 2002-04-15 Isolation of DNA molecules
US10/473,239 US6916919B2 (en) 2001-04-18 2002-04-15 Isolation of DNA molecules
CA2444259A CA2444259C (en) 2001-04-18 2002-04-15 Isolation of dna molecules using mercapto-aryl ligands

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE0101380.4 2001-04-18
SE0101380A SE0101380D0 (en) 2001-04-18 2001-04-18 Isolation of plasmid DNA molecules
SE0103436A SE0103436D0 (en) 2001-10-12 2001-10-12 Isolation of plasmid DNA molecules
SE0103436.2 2001-10-12

Publications (2)

Publication Number Publication Date
WO2002083893A2 WO2002083893A2 (en) 2002-10-24
WO2002083893A3 true WO2002083893A3 (en) 2003-07-31

Family

ID=26655447

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2002/004152 WO2002083893A2 (en) 2001-04-18 2002-04-15 Isolation of dna molecules using mercapto-aryl ligands

Country Status (10)

Country Link
US (1) US6916919B2 (en)
EP (1) EP1379645B1 (en)
JP (1) JP4081376B2 (en)
AT (1) ATE471980T1 (en)
AU (1) AU2002302534B2 (en)
CA (1) CA2444259C (en)
DE (2) DE60236789D1 (en)
ES (1) ES2347533T3 (en)
GB (1) GB2377447B (en)
WO (1) WO2002083893A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0323814D0 (en) * 2003-10-10 2003-11-12 Cobra Biolog Ltd Purification of biological macromolecules
KR100668338B1 (en) 2005-05-21 2007-01-12 삼성전자주식회사 Novel pH dependent ion exchange material a solid substrate having immobilized the material on the surface and a method for isolating a nucleic acid using the material and the solid substrate
WO2008150826A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
JP5463492B2 (en) * 2008-10-08 2014-04-09 島根県 Plasmid DNA extraction from microbial cells
WO2010046918A1 (en) * 2008-10-08 2010-04-29 V. B. Medicare Pvt. Ltd. Purification of supercoiled plasmid dna
KR101871683B1 (en) 2010-07-30 2018-06-27 이엠디 밀리포어 코포레이션 Chromatogrphy media and method
EP3636696A1 (en) 2011-05-04 2020-04-15 Cornell University Multiblock copolymer films, methods of making same and uses thereof
KR102072529B1 (en) 2014-09-02 2020-02-25 이엠디 밀리포어 코포레이션 High surface area fiber media with nano-fibrillated surface features
KR102162753B1 (en) 2014-12-08 2020-10-07 이엠디 밀리포어 코포레이션 Mixed bed ion exchange adsorber
KR102308085B1 (en) 2016-04-28 2021-10-06 테라포어 테크놀로지스, 인코포레이티드 Charged Isoporous Materials for Electrostatic Separation
EP3541500B1 (en) 2016-11-17 2021-12-08 Shethji, Jayraj K. Isoporous self-assembled block copolymer films containing high molecular weight hydrophilic additives and methods of making the same
EP3585506A4 (en) 2017-02-22 2021-01-13 Terapore Technologies, Inc. Ligand bound mbp membranes, uses and method of manufacturing
EP3621722A1 (en) 2017-05-12 2020-03-18 Terapore Technologies, Inc. Chemically resistant fluorinated multiblock polymer structures, methods of manufacturing and use
JP2021517861A (en) 2018-03-12 2021-07-29 テラポア テクノロジーズ，インコーポレイテッド Isoporous mesoporous iso-asymmetric material with macrovoids and its manufacturing method
CN109092263B (en) * 2018-08-28 2021-04-27 武汉瑞法医疗器械有限公司 Low-density lipoprotein adsorbent and preparation method thereof
CN109370963A (en) * 2018-09-21 2019-02-22 安徽欣伯玉生物科技有限公司 A kind of cultural method of colibacillus engineering high efficient expression supercoiled plasmid DNA
CN113969276A (en) * 2020-07-23 2022-01-25 西安桑尼赛尔生物医药有限公司 Gel chromatography method for efficiently purifying large amount of PCR products and application thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0168363A2 (en) * 1984-05-17 1986-01-15 Jerker Porath Sulfone activated thioether adsorbents for the separation of proteins and the like
WO1995031279A1 (en) * 1994-05-16 1995-11-23 Biosepra Inc. Chromatography adsorbents utilizing mercapto heterocyclic ligands
WO1997025139A1 (en) * 1996-01-11 1997-07-17 Massey University Methods for the preparation of resins with ligands attached thereto through a linking group comprising sulfide, sulfoxide, sulfone or sulfonate functionality
WO2001038516A1 (en) * 1999-11-25 2001-05-31 Amersham Biosciences Ab Method for purifying plasmid dna by anion-exchange chromatography

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5217593A (en) * 1991-03-13 1993-06-08 Macconnell William P Nucleic acid purification system and method
US5652348A (en) * 1994-09-23 1997-07-29 Massey University Chromatographic resins and methods for using same
US5660984A (en) * 1994-12-09 1997-08-26 Davis; Thomas E. DNA isolating apparatus comprising a non-porous DNA binding, anion exchange resin and methods of use thereof
EP1683631A1 (en) * 2005-01-25 2006-07-26 Borealis Technology OY Multilayer structures

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0168363A2 (en) * 1984-05-17 1986-01-15 Jerker Porath Sulfone activated thioether adsorbents for the separation of proteins and the like
WO1995031279A1 (en) * 1994-05-16 1995-11-23 Biosepra Inc. Chromatography adsorbents utilizing mercapto heterocyclic ligands
WO1997025139A1 (en) * 1996-01-11 1997-07-17 Massey University Methods for the preparation of resins with ligands attached thereto through a linking group comprising sulfide, sulfoxide, sulfone or sulfonate functionality
WO2001038516A1 (en) * 1999-11-25 2001-05-31 Amersham Biosciences Ab Method for purifying plasmid dna by anion-exchange chromatography

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
LEMMENS R ET AL: "S-ARYL LIGANDS FOR CHROMATOGRAPHIC ISOLATION OF SUPERCOILED PLASMID DNA", 9TH MEETING OF THE EUROPEAN SOCIETY OF GENE THERAPY, ANTALYA, TURKEY, 2 November 2001 (2001-11-02) - 4 November 2001 (2001-11-04), pages 18-1157-80, XP001118032 *
SCHWARZ A ET AL: "Novel heterocyclic ligands for the thiophilic purification of antibodies", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL APPLICATIONS, ELSEVIER SCIENCE PUBLISHERS, NL, vol. 664, no. 1, 3 February 1995 (1995-02-03), pages 83 - 88, XP004043709, ISSN: 0378-4347 *

Also Published As

Publication number Publication date
ATE471980T1 (en) 2010-07-15
EP1379645A2 (en) 2004-01-14
US6916919B2 (en) 2005-07-12
GB0208591D0 (en) 2002-05-22
AU2002302534B2 (en) 2007-09-20
EP1379645B1 (en) 2010-06-23
JP4081376B2 (en) 2008-04-23
CA2444259C (en) 2012-10-02
GB2377447B (en) 2003-06-25
WO2002083893A2 (en) 2002-10-24
JP2004533237A (en) 2004-11-04
ES2347533T3 (en) 2010-11-02
DE60236789D1 (en) 2010-08-05
GB2377447A (en) 2003-01-15
DE10217084A1 (en) 2002-11-21
CA2444259A1 (en) 2002-10-24
US20040126778A1 (en) 2004-07-01

Similar Documents

Publication Publication Date Title
WO2002083893A3 (en) Isolation of dna molecules using mercapto-aryl ligands
US6383393B1 (en) Chromatographic purification and separation process for mixtures of nucleic acids
Khorana Total synthesis of a gene
US9624252B2 (en) Selective nucleic acid fragment recovery
RU2719641C1 (en) Novel methods of producing oligonucleotides
CA2012777C (en) Process for isolating nucleic acid
JP4699868B2 (en) Nucleic acid purification method and nucleic acid purification instrument
Catani et al. Oligonucleotides: current trends and innovative applications in the synthesis, characterization, and purification
ES2463449T3 (en) Procedure for the isolation of nucleic acids from any starting material
Pereira et al. Purification of pre-miR-29 by arginine-affinity chromatography
US20060134638A1 (en) Error reduction in automated gene synthesis
WO2005097205A3 (en) Dna virus microrna and methods for inhibiting same
WO2005054441A3 (en) Device for immobilizing chemical and biomedical species and methods of using same
CN102031258A (en) Method for purifying plasmid DNA
WO1998005673A8 (en) Methods for purifying nucleic acids
WO2003054232A3 (en) Methods for removal of double-stranded oligonucleotides containing sequence errors using mismatch recognition proteins
WO2001025488A3 (en) Method for enrichment of natural antisense messenger rna
JP2006311803A (en) Method for purifying nucleic acid and tool for purifying nucleic acid
AU2003204489B2 (en) Process of extracting nucleic acid and process of simultaneously carrying out extraction and purification of nucleic acid
US20220025354A1 (en) Nucleic acid synthesis and purification device, use thereof, and nucleic acid synthesis and purification method
JP2004504829A (en) Methods for isolating one strand of a double-stranded nucleic acid
Pereira et al. New approach for purification of pre-miR-29 using lysine-affinity chromatography
Sheng et al. Isolating single stranded DNA using a microfluidic dialysis device
WO2001059102A3 (en) Nucleozymes with endonuclease activity
JP2003144149A (en) Isolation apparatus and method of using the same

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG

121 Ep: the epo has been informed by wipo that ep was designated in this application
WWE Wipo information: entry into national phase

Ref document number: 2002730158

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2002302534

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 10473239

Country of ref document: US

WWE Wipo information: entry into national phase

Ref document number: 2444259

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2002582231

Country of ref document: JP

WWP Wipo information: published in national office

Ref document number: 2002730158

Country of ref document: EP

REG Reference to national code

Ref country code: DE

Ref legal event code: 8642