WO2002083133A1 - Novel oral general anesthetics and metabolitically resistant anticonvulsants - Google Patents
Novel oral general anesthetics and metabolitically resistant anticonvulsants Download PDFInfo
- Publication number
- WO2002083133A1 WO2002083133A1 PCT/US2002/011507 US0211507W WO02083133A1 WO 2002083133 A1 WO2002083133 A1 WO 2002083133A1 US 0211507 W US0211507 W US 0211507W WO 02083133 A1 WO02083133 A1 WO 02083133A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- alkyl
- halo
- compound
- group
- independently
- Prior art date
Links
- 0 CC(*)(*)[C@](C(N)=O)(c1ccccc1)O Chemical compound CC(*)(*)[C@](C(N)=O)(c1ccccc1)O 0.000 description 6
- LUXBYZMEMKQMFF-YRNVUSSQSA-N C/C(/C(C(F)(F)F)(C(N)=O)OCc1ccccc1)=C\C=C Chemical compound C/C(/C(C(F)(F)F)(C(N)=O)OCc1ccccc1)=C\C=C LUXBYZMEMKQMFF-YRNVUSSQSA-N 0.000 description 1
- FFXHDJHFAYTIHJ-UHFFFAOYSA-N N#CC(C(F)(F)F)(c1ccccc1)O Chemical compound N#CC(C(F)(F)F)(c1ccccc1)O FFXHDJHFAYTIHJ-UHFFFAOYSA-N 0.000 description 1
- LIDMQHMANOPYMS-UHFFFAOYSA-N NC(C(C(Cl)(Cl)Cl)(c1ccccc1)O)=O Chemical compound NC(C(C(Cl)(Cl)Cl)(c1ccccc1)O)=O LIDMQHMANOPYMS-UHFFFAOYSA-N 0.000 description 1
- IZSYOOVODICBOU-UHFFFAOYSA-N NC(C(C(Cl)(Cl)Cl)=O)=O Chemical compound NC(C(C(Cl)(Cl)Cl)=O)=O IZSYOOVODICBOU-UHFFFAOYSA-N 0.000 description 1
- KCCGYHFRRRMLAU-UHFFFAOYSA-N NC(C(C(F)(F)F)(c1ccccc1)O)=O Chemical compound NC(C(C(F)(F)F)(c1ccccc1)O)=O KCCGYHFRRRMLAU-UHFFFAOYSA-N 0.000 description 1
- ZRGKKWIQLGNOLL-UHFFFAOYSA-N O=C(C(Cl)(Cl)Cl)[ClH]C1CC1 Chemical compound O=C(C(Cl)(Cl)Cl)[ClH]C1CC1 ZRGKKWIQLGNOLL-UHFFFAOYSA-N 0.000 description 1
- KZJRKRQSDZGHEC-UHFFFAOYSA-N O=C(C(F)(F)F)c1ccccc1 Chemical compound O=C(C(F)(F)F)c1ccccc1 KZJRKRQSDZGHEC-UHFFFAOYSA-N 0.000 description 1
- ICTDWYWDPOFBFN-UHFFFAOYSA-N O=C1NCCCC=C1N1CCCCC1 Chemical compound O=C1NCCCC=C1N1CCCCC1 ICTDWYWDPOFBFN-UHFFFAOYSA-N 0.000 description 1
- JBKVHLHDHHXQEQ-UHFFFAOYSA-N O=C1NCCCCC1 Chemical compound O=C1NCCCCC1 JBKVHLHDHHXQEQ-UHFFFAOYSA-N 0.000 description 1
- KJIRIIANVAJNNI-UHFFFAOYSA-N O=C1NCCCCC1(Br)Br Chemical compound O=C1NCCCCC1(Br)Br KJIRIIANVAJNNI-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
- C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
- C07D233/66—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D233/72—Two oxygen atoms, e.g. hydantoin
- C07D233/76—Two oxygen atoms, e.g. hydantoin with substituted hydrocarbon radicals attached to the third ring carbon atom
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/16—Amides, e.g. hydroxamic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/40—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with one nitrogen as the only ring hetero atom, e.g. sulpiride, succinimide, tolmetin, buflomedil
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/415—1,2-Diazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
- A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
- A61K31/445—Non condensed piperidines, e.g. piperocaine
- A61K31/45—Non condensed piperidines, e.g. piperocaine having oxo groups directly attached to the heterocyclic ring, e.g. cycloheximide
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/55—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having seven-membered rings, e.g. azelastine, pentylenetetrazole
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/65—Tetracyclines
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P23/00—Anaesthetics
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C235/00—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms
- C07C235/02—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton
- C07C235/32—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings
- C07C235/34—Carboxylic acid amides, the carbon skeleton of the acid part being further substituted by oxygen atoms having carbon atoms of carboxamide groups bound to acyclic carbon atoms and singly-bound oxygen atoms bound to the same carbon skeleton the carbon skeleton containing six-membered aromatic rings having the nitrogen atoms of the carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/04—Indoles; Hydrogenated indoles
- C07D209/30—Indoles; Hydrogenated indoles with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, directly attached to carbon atoms of the hetero ring
- C07D209/32—Oxygen atoms
- C07D209/38—Oxygen atoms in positions 2 and 3, e.g. isatin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D223/00—Heterocyclic compounds containing seven-membered rings having one nitrogen atom as the only ring hetero atom
- C07D223/02—Heterocyclic compounds containing seven-membered rings having one nitrogen atom as the only ring hetero atom not condensed with other rings
- C07D223/06—Heterocyclic compounds containing seven-membered rings having one nitrogen atom as the only ring hetero atom not condensed with other rings with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D223/08—Oxygen atoms
- C07D223/10—Oxygen atoms attached in position 2
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D233/00—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings
- C07D233/54—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members
- C07D233/66—Heterocyclic compounds containing 1,3-diazole or hydrogenated 1,3-diazole rings, not condensed with other rings having two double bonds between ring members or between ring members and non-ring members with hetero atoms or with carbon atoms having three bonds to hetero atoms with at the most one bond to halogen, e.g. ester or nitrile radicals, directly attached to ring carbon atoms
- C07D233/72—Two oxygen atoms, e.g. hydantoin
- C07D233/74—Two oxygen atoms, e.g. hydantoin with only hydrogen atoms or radicals containing only hydrogen and carbon atoms, attached to other ring members
Definitions
- the present invention is directed to novel derivatives of ⁇ -hydroxy- ⁇ - methylbenzeneacetamide (themisone), and the use of such derivatives as therapeutic agents. More particularly, compositions comprising the present themisone derivatives can be administered for reducing the incidence and severity of seizures and for use as a general anesthetic.
- themisone ⁇ -hydroxy- ⁇ - methylbenzeneacetamide
- compositions are available for sedating patients or, in larger dosages, for inducing surgical anethesia in patients. These materials are used alone or in combination with other agents, such as nitrous oxide, to induce narcosis and to raise the patients pain threshold so that the patient can withstand surgical procedures. Likewise in smaller doses, these materials can reduce anxiety and generally sedate the patient.
- agents such as nitrous oxide
- these materials can reduce anxiety and generally sedate the patient.
- the following compounds are in general use as sedative and anesthetic agents: thiopental sodium, 5-allyl-l-methyl-5-(l-methyl-2-pentynyl) barbituric acid sodium salt (brevitol), 2-bromo-2-chloro-l,l,l-trifloroethane (halothane), and the like.
- General anesthesia administered as an inhaled or intravenous agent, for a surgical operation involves analgesia, amnesia, loss of consciousness, motionlessness and abolition of autonomic responses.
- the mechanism of action is still not completely understood, but most general anesthetics act at multiple molecular sites.
- the potential mechanisms of anesthesia action include protein receptor, lipid and ion channels.
- solubility of anesthetics increases in oil, so does the potency leading to the lipid theory developed by Meyer and Overton.
- anesthesia potency and the ability of the anesthetic to inhibit the enzyme activity of the protein, for example in firefly luciferase, a model used for studying anesthesia.
- GABA gamma-aminobutyric acid
- the present invention is directed to derivatives of themisone, that have been found to have anticonvulsant and anesthetic activity.
- Themisone also known as Atrolactamide, was found in the 1950's to be a very potent anticonvulsant.
- the racemic mixture protected 4 out of 4 mice against seizures at 250 mg/kg, however the compound was toxic (blood dyscrasias, rash). Applicants believe the toxicity of this compound results from the formation of 2-phenyl-acrylamide by an elimination reaction as shown below:
- one aspect of the present invention is directed to novel themisone derivatives that are blocked from forming 2-phenyl-acrylamide and the use of such compounds for reducing the incidence and severity of seizures and for use as a general anesthetic.
- the present invention is directed to compounds having a general structure selected from the group consisting of:
- Fig. 1 is a graph plotting the partition coefficient, P (in log P) vs minimum alveolar concentration (MAC) and representing the anesthetic effects of the themisone derivatives (indicated by a reduction in the threshold of Isoflurane anesthesia).
- Fig. 2 is a table listing the data produced during anticonvulsant testing of orally administered themisone and compound 17 in mice.
- the model examines the compound's ability to stop the spread of seizures induced by a maximal electroshock (MES) test, where corneal electrode implants are primed with a drop of electrolyte solution and an electrical stimulus is delivered for 0.2 second.
- MES maximal electroshock
- Fig. 3 is a table listing the data produced during anticonvulsant testing (using a subcutaneous metrazol test) of infraperitoneal, (i.p.) administered themisone and compound 17 in mice.
- the subcutaneous metrazol test (scMet) is conducted using a convulsant dose of pentylenetetrazol at the peak effect time of the compound.
- Fig. 4 is a table listing the data produced from a toxicity test (TOX). The animals walk on a spinning rod for varying lengths of time to check for the loss of righting reflex or other toxic effects.
- TOX toxicity test
- Fig. 5 is a table listing the MES ED 50 , ScMet ED 50 and Rotorod TD 50 values for mice orally administered either compound 17 or phenytoid.
- Fig. 6 is a graph plotting the heart rate vs dose and representing the effects of the presence or absence of ICM-I-40N (17) on heart rate during Isoflurane anesthesia.
- Fig. 7 is a graph plotting the mean blood pressure vs dose and representing the effects of the presence or absence of ICM-I-40N (17) on blood pressure during Isoflurane anesthesia.
- Fig. 8 is a graph plotting the percent MAC reduction vs dose and representing the reduction in the threshold of Isoflurane anesthesia following the administration of ICM-I-40N (•), ICM-1-76D (O), ICM-1-22 ( ⁇ ) and ICM-1-135 (D).
- purified and like terms relate to the isolation of a molecule or compound in a form that is substantially free of contaminants normally associated with the molecule or compound in a native or natural environment.
- treating includes prophylaxis of the specific disorder or condition, or alleviation of the symptoms associated with a specific disorder or condition and/or preventing or eliminating said symptoms.
- halogen means Cl, Br, F, and I. Especially preferred halogens include Cl, Br, and F.
- haloalkyl refers to a C, -C n alkyl radical bearing at least one halogen substituent, for example, chloromethyl, fluoroethyl or trifluoromethyl and the like.
- C, -C n alkyl wherein n is an integer, as used herein, represents a branched or linear alkyl group having from one to the specified number of carbon atoms.
- C, -C 6 alkyl groups include, but are not limited to, methyl, ethyl, n-propyl, iso-propyl, butyl, iso-butyl, sec-butyl, tert-butyl, pentyl, hexyl and the like.
- C 2 -C n alkenyl wherein n is an integer, as used herein, represents an olefinically unsaturated branched or linear group having from 2 to the specified number of carbon atoms and at least one double bond.
- groups include, but are not limited to, 1-propenyl, 2-propenyl, 1,3-butadienyl, 1- butenyl, hexenyl, pentenyl, and the like.
- C 2 -C n alkynyl wherein n is an integer refers to an unsaturated branched or linear group having from 2 to the specified number of carbon atoms and at least one triple bond. Examples of such groups include, but are not limited to, 1-propynyl, 2-propynyl, 1-butynyl, 2-butynyl, 1-pentynyl, and the like.
- C 3 -C n cycloalkyl wherein n is an integer refers to cyclic non-aryl group, for example C 3 -C 8 cycloalkyl, represents cyciopropyi, cyclobutyi, cyclopentyl, cyclohexyl, cycloheptyl, and cyclooctyl.
- lower alkyl refers to branched or straight chain alkyl groups comprising one to eight carbon atoms, including methyl, ethyl, propyl, isopropyl, n-butyl, t-butyl, neopentyl and the like.
- the term "optionally substituted” refers to from zero to four substituents, wherein the substituents are each independently selected. Each of the independently selected substituents may be the same or different than other substituents.
- aryl refers to a mono- or bicyclic carbocyclic ring system having one or two aromatic rings including, but not limited to, phenyl, benzyl, naphthyl, tetrahydronaphthyl, indanyl, indenyl, and the like.
- Optionally substituted aryl includes aryl compounds having from zero to four substituents, and “substituted aryl” includes aryl compounds having one to three substituents.
- the term (C 5 -C 8 alkyl)aryl refers to any aryl group which is attached to the parent moiety via the alkyl group.
- heterocyclic group refers to a mono- or bicyclic carbocyclic ring system containing from one to three heteroatoms wherein the heteroatoms are selected from the group consisting of oxygen, sulfur, and nitrogen.
- heteroaryl refers to a mono- or bicyclic carbocyclic ring system having one or two aromatic rings containing from one to three heteroatoms and includes, but is not limited to, furyl, thienyl, pyridyl and the like.
- the term "pharmaceutically acceptable carrier” encompasses any of the standard pharmaceutical carriers, such as a phosphate buffered saline solution, water and emulsions such as an oil/water or water/oil emulsion, and various types of wetting agents.
- an effective amount means an amount sufficient to produce a selected effect.
- an effective amount of an anticonvulsant themisone derivative is an amount of the compound sufficient to reduce the incidence of seizures in a patient receiving the dose amount.
- parenteral means not through the alimentary canal but by some other route such as subcutaneous, intramuscular, intraspinal, or intravenous.
- Neurological disease or “neurological condition” includes neurological related maladies such as spasticity, seizures, depression or mood disorders, neuropathic pain, Alzheimer's Disease, Parkinson's Disease, HIV Dementia and neurological disorders that involve excessive activation of the N-methyl-D-aspartate (NMDA) receptor.
- NMDA N-methyl-D-aspartate
- Compounds of the present invention that have one or more asymmetric carbon atoms may exist as the optically pure enantiomers, or optically pure diastereomers, as well as mixtures of enantiomers, mixtures of diastereomers, and racemic mixtures of such stereoisomers.
- the present invention includes within its scope all such isomers and mixtures thereof.
- the MAC value is the minimum alveolar concentration of anesthetic at 1 atm that produces immobility in 50% of the subjects.
- the themisone derivatives of the present invention exhibit similar activities to themisone without, the risk of the toxicities associated with themisone administration. Furthermore, applicants have discovered that the themisone derivatives of the present invention also have activity as anesthetics.
- W is selected from the group consisting of alkyl, alkenyl, alkynyl optionally substituted aryl, and optionally substituted heteroaryl;
- Z is hydroxy, alkoxy, -OCOR 12 , or, -COR 12 ;
- R is selected from the group consisting of H, halo, C,-C 4 haloalkyl, -NH 2 , hydroxy, and C,-C 4 alkoxy;
- R 4 is selected from the group consisting of C,-C 4 alkyl, aryl and H;
- R 10 is H or C,-C 4 alkyl;
- R 12 is C,-C 4 alkyl, NH 2 or aryl; m is an integer ranging from 0-3; and n is an integer ranging from 0-1. More particularly, the present invention is directed to a compound represented by a formula selected from the group consisting of:
- R and R, 5 are independently selected from the group consisting of H, halo, C,-C 4 haloalkyl, -NH 2 , hydroxy, and C,-C 4 alkoxy;
- Z is hydroxy or alkoxy
- R 3 is aryl, carboxyl, haloalkyl, -(C r C 4 alkyl)NHR 4 , -CONR 10 R 4 or H;
- R 4 is selected from the group consisting of C,-C 4 alkyl, aryl and H;
- R 6 , R 7 and R 8 are independently halo
- R 9 is optionally substituted aryl or haloalkyl; m is an integer ranging from 0-1 ; and n is an integer ranging from 0-1.
- the themisone derived compound has the general structure:
- R is H or halo
- R 10 and R 5 are independently H, C,-C 4 alkyl, or R 10 and R 5 taken together, can form with the adjacent ring, an optionally substituted 5- or 6-membered heterocyclic ring;
- R 4 is selected from the group consisting of C,-C 4 alkyl, aryl and H;
- R 6 , R 7 and R g are independently halo; and n is an integer ranging from 0-1. More preferably, R* is halo, R 10 , R 4 and R 5 are H, n is 0; and R ⁇ , R 7 and R 8 are independently selected from the group consisting of F, Cl and Br. In one preferred embodiment the R, substituent is in the meta or para position and R ⁇ , R 7 and R 8 are each F.
- R ⁇ is selected from the group consisting of C,-C 4 alkyl, aryl and H; and R ⁇ , R 7 and R 8 are independently fluorine or chlorine.
- R ⁇ is H or C,-C 4 alkyl and R 6 , R 7 and Rg are each fluorine
- the themisone derivatives of the present invention can be formulated as pharmaceutical compositions by combining the compounds with one or more pharmaceutically acceptable carriers, fillers, solubilizing agents and stabilizers known to those skilled in the art.
- Such pharmaceutical compositions can be utilized as analgesics, sedatives, anesthetics or as anticonvulsants.
- compositions comprising the themisone derivatives of the present invention are administered to an individual in need thereof by any number of routes including, but not limited to, topical, oral, intravenous, intramuscular, intra- arterial, intramedullary, intrathecal, intraventricular, transdermal, subcutaneous, intraperitoneal, intranasal, enteral, topical, sublingual, or rectal means, with oral and intravenous routes being preferred.
- the compounds can be administered as a liquid solution, powder (lyophilized or otherwise), tablet, capsule or lozenge.
- oral formulations may include one or more of the present compounds in combination with one or more conventional pharmaceutical additive or excipients that are typically used in the preparation of tablets, capsules, lozenges and other orally administrable forms.
- compositions according to the present invention may comprise a themisone derivative and phencyclidine type general anesthetic such as ketamine or tiletamine and their pharmaceutically acceptable salts, as well as selegiline or one of its pharmaceutically acceptable salts, combined in a single pharmaceutical composition for simultaneous administration, or presented separately for administration in close succession.
- selegiline has the role of pre- anesthetic or restraining agent.
- Tiletamine is 2-(ethylamino)-2-(2- thienyl)cyclohexanone.
- Ketamine is (+-)-2-(2-chlorophenyl)-2-methyl- aminocyclohexanone.
- the present invention also provides a pharmaceutical pack or kit comprising one or more containers filled with one or more of the themisone derivatives of the present invention.
- a kit is provided for anesthetizing a patient.
- the kit may comprise one or more anesthetic agents of the present invention and as well as other known anesthetic agents and pre-anesthetic or restraining agents.
- These pharmaceuticals can be packaged in a variety of containers, e.g. , vials, tubes, microtiter well plates, bottles, and the like.
- the kits will also include instructions for use.
- compositions comprising a themisone derivative of the present invention are used as a general anesthesia in mammals, including both human and domesticated animals. More particularly, compositions comprising the present themisone derivative are administered either orally or parenterally to a mammalian species to induce anesthesia.
- the compounds When administered orally, the compounds are administered as a liquid solution, powder, tablet, capsule or lozenge.
- the compounds can be used in combination with one or more conventional pharmaceutical additive or excipients used in the preparation of tablets, capsules, lozenges and other orally administrable forms.
- the derivatives of the present invention can be admixed with saline solutions and/or conventional IV solutions.
- a method is provided for inducing anesthesia in a human patient. The method comprises the steps of administering to the patient a composition comprising a compound represented by a formula selected from the group consisting of
- R is selected from the group consisting of H, halo, C,-C 4 haloalkyl, -NH 2 , hydroxy, and C,-C 4 alkoxy;
- R 2 , R 3 and R 4 are independently H or alkyl
- R 6 , R 7 and R 8 are independently halo; and n is an integer ranging from 0-4, and a pharmaceutically acceptable carrier.
- a pharmaceutical composition comprising a compound of the general formula:
- R is selected from the group consisting of H or halo and Rg, R 7 and R 8 are independently H or halo, with the proviso that at least one and more preferably two of R t , R 7 and R 8 are halo. More preferably R, is F or Br and R ⁇ R 7 and R 8 are independently F or Cl.
- a method for treating a neurological condition including the treatment of seizures.
- the method comprises the steps of administering to a patient a composition comprising a compound represented by a formula selected from the group consisting of
- R is selected from the group consisting of H, halo,C,-C 4 haloalkyl, -NH 2 , hydroxy, and C,-C 4 alkoxy;
- R 2 and R 4 are independently H or alkyl
- R ]0 and R 5 are independently H, alkyl, or R 10 and R 5 taken together, can form with the adjacent ring, an optionally substituted 5- or 6-membered heterocyclic ring;
- R is optionally substituted aryl or haloalkyl; n is an integer ranging from 0-2; and R 13 is haloalkyl. More preferably, R, is H or halo, R 2 is H and n is 0.
- R is H or halo
- R 4 is H or alkyl
- R 10 and R 5 are independently H, alkyl, or R 10 and R 5 taken together, can form with the adjacent ring, an optionally substituted 5- or 6-membered heterocyclic ring
- R 13 is haloalkyl
- R 14 is C,-C 12 alkyl. More preferably, R, is H or halo, R 4 , R 5 and R 10 are H and R 14 is C 4 -C 6 alkyl. In one embodiment these compounds are administered to a patient as a treatment for reducing the incidence and severity of seizures.
- the hydrogens of the 2-methyl group substituent of themisone are substituted with halo groups to prevent the elimination reaction and formation of 2-phenyl-acrylamide.
- the hydrogens can be replaced with fluorine to create 3,3,3-Trifluoro-2-hydroxy-2-phenyl- propionamide (17).
- Fluorine is an classic mimic for hydrogen, with the two elements having similar Van der Waals radii, 1.20 and 1.35 angstroms, respectively.
- the tested animals exhibited surgical anesthesia, the third stage of anesthesia displayed by general loss of spinal reflexes and muscle tone.
- Themisone was later tested and found to have no anesthetic activity.
- Other themisone compounds have now been tested and found to exhibit both anticonvulsant and anesthetic activities as described in the following examples.
- Compound 39 also demonstrated activity as an anticonvulsant both in the MES and scMET assays (See Example 3).
- 4 out of 4 rats were protected using 30mg/kg for a duration of 2 hours.
- intraparentoneal data for MES 2 out of 3 mice were protected at 100 mg/kg for a duration of 1 hour and 5out of 5 mice were protected at lOOmg/kg for a duration of 0.5 hours.
- the formation of 2-phenyl-acrylamide is blocked by replacing the hydrogens of the 2- methyl group substituent of themisone with compounds that prevent the elimination reaction.
- the hydrogens can be replaced with fluorine to create 3,3,3- Trifluoro-2-hydroxy-2-phenyl-propionamide (17).
- Reaction conditions a: TMSCN,TiCl 4 , CH 2 C1 2 ; b: H 2 O; c: 1,4-dioxane, cone. HCl, HCl gas.
- Rxn Conditions a: piperdine, Et 3 N, Ether; b: 4-Bromoanisole, Mg, I 2 , THF; c: NH 4 C1 Halogenation of general anesthetics stabilizes the compounds and reduces metabolism. We hypothesized that changes in the haloform content could have an affect on potency, duration and hemodynamic acitivty.
- the chlorinated cyanohydrin 28 was successfully made (see Scheme V), although several attempts to synthesize the hydroxyamide were unsuccessful. Under acidic conditions such as HCl and HCl gas, or formic acid and HCl gas converted the cyanohydrin back to the starting ketone. Other unsuccessful attempts included K2CO3 and 30% H2O2, and
- Reaction Conditions a: acetophenone, acetic acid, reflux, 8 hrs.; b: TMS-CN, THF, reflux, 12 hrs.; c: 15% HCl; d: cone. HCl, HCl gas; e: formic acid, HCl gas; f: K 2 CO 3 , 30% H 2 O 2 ; g: UHP, K 2 CO 3 .
- Reaction Conditions a: Br 2 , NaOH, H 2 O, 1,4-dioxane, 0°C to RT; b: TMS-CN, Znl 2 , CH 2 C1 2 ; c: TMS-CN, KCN, 18-crown-6, CH 2 C1 2 .
- 200 mg of each compound are tested for general anesthesia activity. Testing is performed on rats to determine the percent reduction in the use of isoflurane. Inhaled anesthetics still need to be utilized to rapidly adjust the level of anesthesia. Since no single anesthetic is capable of all four requirements for anesthesia, a combination is utilized. The rats were first anesthesized with isoflurane, intubated, ventilated and then a femoral line was inserted. After the rat had been stable for 15 minutes, the initial minimum alveolar concentration (MAC) value was determined. Then, 60 mg/kg of drug was sonicated in 3 mL of peanut oil and injected (i.p.). After 30 minutes the second MAC value was determined post administration.
- MAC alveolar concentration
- a graph representing the effects of log P on the biological activity is presented in graphic form (See Fig 1).
- the general anesthetic activity of compounds 20-23 and 36- 43 were compared to lead compound 17 as represented in the graph (Fig. 1) and in Table 1.
- the partition coefficient, P is a measure of the way the compound distributes itself between octanol and water.
- the graph displays a general parabola shape with an optimal log P value of approximately 1.5, since compounds 20 and 42 were very toxic, despite their high activity.
- Compounds 20 and 42 also had a low lethal dose of 300 mg/kg.
- Derivatives with para- substituted phenol ring derivatives 21 and 39 were found to be very active, but also toxic at the same dosage. Therefore, meta-substituted derivatives 20 will be the focus of future synthesize.
- Bulky substituents on the alcohol were also found to have low activity. Closed ring analogs are active at lower levels but cause no hemodynamic effects.
- Table 1 Table 1:
- -CF3 The phenyl-substituted caprolactams have been found to be very active anticonvulsants. Therefore, the -CF3 moiety was added to the seven membered ring carpolactam (see Scheme VIII) and these compounds are anticipated to have activity as anticonvulsants.
- -Caprolactam is alpha brominated with bromine and phosphorus pentachloride. Formation of the enamine 31 is accomplished with 30 refluxed in piperdine for 6 hours. Cleavage of the enamine is done on a silica gel column eluted with ethyl acetate. Due to the low yield of 32 the amide was protected before the reaction with TMS-CF3. There were several unsuccessful attempts to protect the amide, using benzyl bromide and various bases.
- NIH National Institute of Health's Anticonvulsant Screening Project of the Antiepileptic Drug Discovery Program.
- NIH performs anticonvulsant testing (oral and infraperitoneal, i.p.) on both mice and rats in phase I trials.
- the grand mal model is conducted with a maximal electroshock (MES) test, where corneal electrode implants are primed with a drop of electrolyte solution and an electrical stimulus is delivered for 0.2 second.
- MES maximal electroshock
- the model examines the compounds' ability to stop the spread of seizures.
- the petite mal model is conducted with a subcutaneous pentylenetetrazol seizure threshold (scMet) test.
- MES maximal electroshock
- scMet subcutaneous pentylenetetrazol seizure threshold
- the animals are injected with a convulsant dose of pentylenetetrazol at the peak effect time of the compound.
- This model measures the compounds' threshold for seizures.
- a toxicity test (TOX) is performed where the animals walk on a spinning rod for varying lengths of time to check for the loss of righting reflex or other toxic effects.
- Phase I evaluation at 30, 100 and 300 mg/kg of test compounds has resulted in several newly discovered active anticonvulsants (See Figs. 2-5).
- Reaction Conditions a: KCN, (NH4)2CO3, 50 % EtOH, 65°C, 24 hrs.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Neurosurgery (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Neurology (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Anesthesiology (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Other In-Based Heterocyclic Compounds (AREA)
- Indole Compounds (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2002580937A JP2004534014A (en) | 2001-04-16 | 2002-04-15 | New oral general anesthetics and metabolic resistant anticonvulsants |
US10/475,545 US7026358B2 (en) | 2001-04-16 | 2002-04-15 | Oral general anesthetics and metabolitically resistant anticonvulsants |
EP02721724A EP1385517A1 (en) | 2001-04-16 | 2002-04-15 | Novel oral general anesthetics and metabolitically resistant anticonvulsants |
CA002443906A CA2443906A1 (en) | 2001-04-16 | 2002-04-15 | Novel oral general anesthetics and metabolitically resistant anticonvulsants |
US11/283,333 US7414078B2 (en) | 2001-04-16 | 2005-11-18 | Oral general anesthetics and metabolitically resistant anticonvulsants |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US28404001P | 2001-04-16 | 2001-04-16 | |
US60/284,040 | 2001-04-16 | ||
US33360301P | 2001-11-27 | 2001-11-27 | |
US60/333,603 | 2001-11-27 | ||
US35418102P | 2002-02-04 | 2002-02-04 | |
US60/354,181 | 2002-02-04 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10475545 A-371-Of-International | 2002-04-15 | ||
US11/283,333 Continuation US7414078B2 (en) | 2001-04-16 | 2005-11-18 | Oral general anesthetics and metabolitically resistant anticonvulsants |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2002083133A1 true WO2002083133A1 (en) | 2002-10-24 |
Family
ID=27403442
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2002/011507 WO2002083133A1 (en) | 2001-04-16 | 2002-04-15 | Novel oral general anesthetics and metabolitically resistant anticonvulsants |
Country Status (6)
Country | Link |
---|---|
US (2) | US7026358B2 (en) |
EP (1) | EP1385517A1 (en) |
JP (1) | JP2004534014A (en) |
CN (1) | CN1516586A (en) |
CA (1) | CA2443906A1 (en) |
WO (1) | WO2002083133A1 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1501790A2 (en) * | 2002-04-18 | 2005-02-02 | University Of Virginia Patent Foundation | Novel sodium channel blockers |
WO2007014843A1 (en) * | 2005-07-28 | 2007-02-08 | F. Hoffmann-La Roche Ag | 2-hydroxy-propionic acid derivatives and 3-hydroxy-benzofuran-2-one derivatives with affinity for the gaba-b-receptor |
WO2011051455A1 (en) * | 2009-10-30 | 2011-05-05 | Bayer Cropscience Ag | Pesticidal heterocyclic compounds |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2008094319A2 (en) * | 2007-02-01 | 2008-08-07 | The Board Of Regents Of The University Of Texas Sytem | Methods and compositions of trail-death receptor agonists/activators |
JP2022126890A (en) * | 2019-07-02 | 2022-08-31 | Agc株式会社 | Fluoroalkyl group-containing compound and method for producing the same |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3287213A (en) * | 1965-06-30 | 1966-11-22 | Janssen Pharmaceutica Nv | Method of combatting arachnids and fungi |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2358890A1 (en) * | 1976-05-24 | 1978-02-17 | Science Union & Cie | NEW ARYL TRIFLUOROETHYLAMINES, THEIR METHODS OF OBTAINING AND THE PHARMACEUTICAL COMPOSITIONS CONTAINING |
ZA953078B (en) * | 1994-04-28 | 1996-01-05 | Alza Corp | Effective therapy for epilepsies |
DE19529425A1 (en) | 1995-08-10 | 1997-02-13 | Dresden Arzneimittel | New, anticonvulsant imidazolin-2,4-diones, which contain an ortho-substituted ar (alk) yl radical in the 1-position, and process for their preparation |
WO1998013336A1 (en) | 1996-09-27 | 1998-04-02 | Research Corporation Technologies, Inc. | Propionamide derivatives and their use as anticonvulsants |
EP1501790A4 (en) * | 2002-04-18 | 2006-03-29 | Univ Virginia | Novel sodium channel blockers |
-
2002
- 2002-04-15 CN CNA028119770A patent/CN1516586A/en active Pending
- 2002-04-15 JP JP2002580937A patent/JP2004534014A/en active Pending
- 2002-04-15 CA CA002443906A patent/CA2443906A1/en not_active Abandoned
- 2002-04-15 EP EP02721724A patent/EP1385517A1/en not_active Withdrawn
- 2002-04-15 US US10/475,545 patent/US7026358B2/en not_active Expired - Lifetime
- 2002-04-15 WO PCT/US2002/011507 patent/WO2002083133A1/en not_active Application Discontinuation
-
2005
- 2005-11-18 US US11/283,333 patent/US7414078B2/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3287213A (en) * | 1965-06-30 | 1966-11-22 | Janssen Pharmaceutica Nv | Method of combatting arachnids and fungi |
Non-Patent Citations (4)
Title |
---|
BOWMAN ET AL.: "Trihalogenomethyl compounds of potential therapeutic interest", J. CHEM. SOC., October 1964 (1964-10-01), pages 3841 - 3845, XP002954826 * |
BURGER ET AL.: "2-Trifluoromethyl alpha amino acid esters, building blocks for trifluoromethyl-substituted ureas, thioureas and other potential biological active structures", LIBIGS. ANN. CHEM., vol. 4, January 1994 (1994-01-01), pages 399 - 406, XP002954827 * |
CARR ET AL.: "3-Halo 2-phenylglycidiamide hypnotics, 3-halo-2-phenylglycidamide hypnotics", vol. 12, November 1969 (1969-11-01), pages 989 - 994, XP002954828 * |
DALE ET AL.: "Alpha methoxy-alpha-trifluoromethylphenylacetic acid, a versatile reagent for the determination of enantiomeric composition of alcohols and amines", THE JOURNAL OF ORGANIC CHEMISTRY, vol. 34, no. 9, September 1969 (1969-09-01), pages 2543 - 2549, XP002954829 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1501790A2 (en) * | 2002-04-18 | 2005-02-02 | University Of Virginia Patent Foundation | Novel sodium channel blockers |
EP1501790A4 (en) * | 2002-04-18 | 2006-03-29 | Univ Virginia | Novel sodium channel blockers |
US7439383B2 (en) | 2002-04-18 | 2008-10-21 | University Of Virginia Patent Foundation | Sodium channel blockers |
US8350076B2 (en) | 2002-04-18 | 2013-01-08 | University Of Virginia Patent Foundation | Sodium channel blockers |
WO2007014843A1 (en) * | 2005-07-28 | 2007-02-08 | F. Hoffmann-La Roche Ag | 2-hydroxy-propionic acid derivatives and 3-hydroxy-benzofuran-2-one derivatives with affinity for the gaba-b-receptor |
US7396853B2 (en) | 2005-07-28 | 2008-07-08 | Hoffmann-La Roche Inc. | 2-phenyl-3,3,3-trifluoro-2-hydroxy-propionic acid derivatives |
US7504428B2 (en) | 2005-07-28 | 2009-03-17 | Hoffman-La Roche Inc. | 2-phenyl-3,3,3-trifluoro-2-hydroxy-propionic acid derivatives |
WO2011051455A1 (en) * | 2009-10-30 | 2011-05-05 | Bayer Cropscience Ag | Pesticidal heterocyclic compounds |
Also Published As
Publication number | Publication date |
---|---|
US7026358B2 (en) | 2006-04-11 |
CA2443906A1 (en) | 2002-10-24 |
CN1516586A (en) | 2004-07-28 |
US20040133012A1 (en) | 2004-07-08 |
US7414078B2 (en) | 2008-08-19 |
US20060111339A1 (en) | 2006-05-25 |
EP1385517A1 (en) | 2004-02-04 |
JP2004534014A (en) | 2004-11-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP6196986B2 (en) | Anesthetic compounds and related methods of use | |
JP5049267B2 (en) | Benzisoxazole piperazine compounds and methods of use thereof | |
JP5925121B2 (en) | Etomidate analogs that do not inhibit corticosteroid synthesis | |
JPH05508422A (en) | 4-hydroxybutyric acid derivative | |
EP2027854A1 (en) | Analgesic uses of norketamine and ketamine/norketamine prodrugs | |
US20090061024A1 (en) | Compositions and methods employing nmda antagonists for achieving an anesthetic-sparing effect | |
WO2012166909A1 (en) | Compositions and methods for treating breathing control disorders or diseases | |
CA2303815A1 (en) | N,n-bis (phenylcarbamoylmethyl) dimethylammonium chloride and derivatives in the treatment of pain | |
US7414078B2 (en) | Oral general anesthetics and metabolitically resistant anticonvulsants | |
JPH09501653A (en) | Pharmaceutical composition | |
BR112020019325A2 (en) | METHOD OF TREATING FIBROTIC DISEASE | |
WO2004000327A1 (en) | Therapeutic agent for type 2 diabetes | |
PT92799B (en) | Use of a partial glycine agonist B, in particular an amino-isoxazole derivative compound for the preparation of a medicament as an antipsychotic and a method for the preparation of a pharmaceutical composition comprising the said agonist | |
TW201320998A (en) | Dosage regimen for an S1P receptor modulator or agonist | |
LV12918B (en) | Use of ropivacaine in the manufacture of a pharmaceutical with analgetic effect with minimal motor blockade | |
KR20050044396A (en) | Monohydroxycarbamazepine for use in the preparation of a medicament for the treatment of affective and attention disorder and neuropathic pain | |
EP1793821B1 (en) | Pyrazolone compounds for treating cerebrovascular disorders | |
García et al. | Pharmacology of intravenous anesthetics | |
US4562206A (en) | Orally effective inotropic compounds | |
Pascoe et al. | Introduction to drugs acting on the central nervous system and principles of anesthesiology | |
CN1057758C (en) | Antiarrhythmic (S) -enantiomers of methanesulfonamides | |
Portenoy | Pharmacologic approaches to the control of cancer pain | |
US20240139140A9 (en) | Compositions and methods for stimulating ventilatory and/or respiratory drive | |
JPH02288823A (en) | Muscle relaxant | |
WO2005016331A1 (en) | Therapeutic agent for neuropathic pain containing n-(benzoyl)amino acid derivative as active ingredient |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
AK | Designated states |
Kind code of ref document: A1 Designated state(s): AE AG AL AM AT AU AZ BA BB BG BR BY BZ CA CH CN CO CR CU CZ DE DK DM DZ EC EE ES FI GB GD GE GH GM HR HU ID IL IN IS JP KE KG KP KR KZ LC LK LR LS LT LU LV MA MD MG MK MN MW MX MZ NO NZ OM PH PL PT RO RU SD SE SG SI SK SL TJ TM TN TR TT TZ UA UG US UZ VN YU ZA ZM ZW |
|
AL | Designated countries for regional patents |
Kind code of ref document: A1 Designated state(s): GH GM KE LS MW MZ SD SL SZ TZ UG ZM ZW AM AZ BY KG KZ MD RU TJ TM AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR BF BJ CF CG CI CM GA GN GQ GW ML MR NE SN TD TG |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application | ||
DFPE | Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101) | ||
WWE | Wipo information: entry into national phase |
Ref document number: 2443906 Country of ref document: CA Ref document number: 01309/KOLNP/2003 Country of ref document: IN Ref document number: 1309/KOLNP/2003 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 10475545 Country of ref document: US |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2002580937 Country of ref document: JP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2002721724 Country of ref document: EP |
|
WWE | Wipo information: entry into national phase |
Ref document number: 028119770 Country of ref document: CN |
|
WWP | Wipo information: published in national office |
Ref document number: 2002721724 Country of ref document: EP |
|
REG | Reference to national code |
Ref country code: DE Ref legal event code: 8642 |
|
WWW | Wipo information: withdrawn in national office |
Ref document number: 2002721724 Country of ref document: EP |