WO2002071061A3 - Quantitative detection of the conformation changes of p-glycoprotein - Google Patents

Quantitative detection of the conformation changes of p-glycoprotein Download PDF

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Publication number
WO2002071061A3
WO2002071061A3 PCT/HU2002/000025 HU0200025W WO02071061A3 WO 2002071061 A3 WO2002071061 A3 WO 2002071061A3 HU 0200025 W HU0200025 W HU 0200025W WO 02071061 A3 WO02071061 A3 WO 02071061A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection
pumps
proteins
receptors
tested
Prior art date
Application number
PCT/HU2002/000025
Other languages
French (fr)
Other versions
WO2002071061A2 (en
Inventor
Gabor Szabo
Gyoergy Lustyik
Henrietta Nagy
Katalin Goda
Original Assignee
Gabor Szabo
Gyoergy Lustyik
Henrietta Nagy
Katalin Goda
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gabor Szabo, Gyoergy Lustyik, Henrietta Nagy, Katalin Goda filed Critical Gabor Szabo
Priority to CA002439713A priority Critical patent/CA2439713A1/en
Priority to EP02707028A priority patent/EP1373891A2/en
Priority to AU2002241185A priority patent/AU2002241185A1/en
Priority to JP2002569932A priority patent/JP2004526148A/en
Priority to US10/469,720 priority patent/US20040137532A1/en
Publication of WO2002071061A2 publication Critical patent/WO2002071061A2/en
Publication of WO2002071061A3 publication Critical patent/WO2002071061A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/566—Immunoassay; Biospecific binding assay; Materials therefor using specific carrier or receptor proteins as ligand binding reagents where possible specific carrier or receptor proteins are classified with their target compounds
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/10—Screening for compounds of potential therapeutic value involving cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00—Detection or diagnosis of diseases
    • G01N2800/52—Predicting or monitoring the response to treatment, e.g. for selection of therapy based on assay results in personalised medicine; Prognosis

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The invention relates to the detection of the presence and/or conformation change of the cell surface receptors, pumps or proteins, when two different antibodies and optionally ligands are bound to the receptors, pumps and/or proteins recognized by the antibodies, and the change in the antibody-binding is measured. The method can be used for the determination of cytostatic drug combination, effective in case of multidrug resistance wherein the native cells are combined in vitro with the drug or drug combination to be tested, and the sample is tested with the method mentioned above. The method can be used for the detection of the efficiency of multidrug-resistance modulators wherein the method according is carried out in the presence of known modulator or a modulator to be tested, and the resulting activity values are compared. Also the object of the invention is a diagnostic kit for the detection of the presence and conformation change of receptors, pumps and proteins, for the determination of the efficient drug combination in case of multidrug resistance and/or for the detection of the efficiency of the modulators comprising a first antibody which is able to detect the receptors, pumps or proteins, optionally other ligands, and a system, which provides a system for the detection of binding.
PCT/HU2002/000025 2001-03-02 2002-03-01 Quantitative detection of the conformation changes of p-glycoprotein WO2002071061A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
CA002439713A CA2439713A1 (en) 2001-03-02 2002-03-01 Quantitative detection of the conformation changes of p-glycoprotein
EP02707028A EP1373891A2 (en) 2001-03-02 2002-03-01 Quantitative detection of the conformation changes of p-glycoprotein
AU2002241185A AU2002241185A1 (en) 2001-03-02 2002-03-01 Quantitative detection of the conformation changes of p-glycoprotein
JP2002569932A JP2004526148A (en) 2001-03-02 2002-03-01 Antibody Competition Test (ACT) for qualitative and quantitative detection of conformational changes in P-glycoprotein and other cell surface receptors, pumps, and proteins, and their use
US10/469,720 US20040137532A1 (en) 2001-03-02 2002-03-01 Antibody competition test (act) for the qualitative and conformation dependent quantitative detection of the conformation changes of p-glycoprotein and other cell surface receptors, pumps and proteins, and uses thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
HU0100948A HUP0100948A2 (en) 2001-03-02 2001-03-02 Antibody competiton binding assay for qualitative detection of the conformation changes and conformation-dependent quantitative detection of glycoprotein p and other cell surface receptors, pumps and proteins, and uses thereof
HUP0100948 2001-03-02

Publications (2)

Publication Number Publication Date
WO2002071061A2 WO2002071061A2 (en) 2002-09-12
WO2002071061A3 true WO2002071061A3 (en) 2003-04-03

Family

ID=89979091

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/HU2002/000025 WO2002071061A2 (en) 2001-03-02 2002-03-01 Quantitative detection of the conformation changes of p-glycoprotein

Country Status (8)

Country Link
US (1) US20040137532A1 (en)
EP (1) EP1373891A2 (en)
JP (1) JP2004526148A (en)
CN (1) CN1703620A (en)
AU (1) AU2002241185A1 (en)
CA (1) CA2439713A1 (en)
HU (1) HUP0100948A2 (en)
WO (1) WO2002071061A2 (en)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2509902A1 (en) 2002-12-13 2004-07-01 Aurelium Biopharma Inc. Nucleophosmin directed diagnostics and therapeutics for multidrug resistant neoplastic disease
US7670604B2 (en) 2002-12-13 2010-03-02 Aurelium Biopharma, Inc. Vimentin directed diagnostics and therapeutics for multidrug resistant neoplastic disease
US7550256B2 (en) 2002-12-13 2009-06-23 Aurelium Biopharma, Inc. Vimentin directed diagnostics and therapeutics for multidrug resistant neoplastic disease
WO2004061458A2 (en) * 2003-01-03 2004-07-22 Aurelium Biopharma Inc. Hsc70 directed diagnostics and therapeutics for multidrug resistant neoplastic disease
US7358042B2 (en) 2003-03-14 2008-04-15 Aurelium Biopharma, Inc. Triosephosphate isomerase directed diagnostics and therapeutics for multidrug resistant neoplastic disease
EP1797436A2 (en) * 2004-10-08 2007-06-20 Cedars-Sinai Medical Center Biological microbeads for various flow cytometric applications
WO2013142003A1 (en) * 2012-03-23 2013-09-26 Laboratory Corporation Of America Holdings Biologic machines for the detection of biomolecules

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334584A (en) * 1989-02-14 1994-08-02 Incyte Pharamaceuticals, Inc. Recombinant, non-glycosylated bpi protein and uses thereof
WO1998021325A1 (en) * 1996-11-15 1998-05-22 The Board Of Trustees Of The University Of Illinois Methods and reagents for preparing and using immunological agents specific for p-glycoprotein

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5334584A (en) * 1989-02-14 1994-08-02 Incyte Pharamaceuticals, Inc. Recombinant, non-glycosylated bpi protein and uses thereof
WO1998021325A1 (en) * 1996-11-15 1998-05-22 The Board Of Trustees Of The University Of Illinois Methods and reagents for preparing and using immunological agents specific for p-glycoprotein

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
GODA KATALIN ET AL: "Conformational heterogeneity of P-glycoprotein.", CANCER DETECTION AND PREVENTION, vol. 24, no. 5, 2000, pages 415 - 421, XP009002077, ISSN: 0361-090X *
MECHETNER E B ET AL: "P-GLYCOPROTEIN FUNCTION INVOLVES CONFORMATIONAL TRANSITIONS DETECTABLE BY DIFFERENTIAL IMMUNOREACTIVITY", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 94, 1 November 1997 (1997-11-01), pages 12908 - 12913, XP002060369, ISSN: 0027-8424 *
NAGY HENRIETTA ET AL: "P-glycoprotein conformational changes detected by antibody competition.", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 268, no. 8, April 2001 (2001-04-01), pages 2416 - 2420, XP002222903, ISSN: 0014-2956 *
SZABO G. ET AL.: "P-glycoprotein conformational changes detected by antibody competition.", PROCEEDINGS OF THE AMERICAN ASOCIATION FOR CANCER RESEARCH ANNUAL MEETING, vol. 42, 24 March 2001 (2001-03-24) - 28 March 2001 (2001-03-28), pages 920, XP001120376 *

Also Published As

Publication number Publication date
CN1703620A (en) 2005-11-30
JP2004526148A (en) 2004-08-26
WO2002071061A2 (en) 2002-09-12
US20040137532A1 (en) 2004-07-15
EP1373891A2 (en) 2004-01-02
HUP0100948A2 (en) 2003-02-28
CA2439713A1 (en) 2002-09-12
AU2002241185A1 (en) 2002-09-19
HU0100948D0 (en) 2001-05-28

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