WO2002066622A3 - Method of generating diversity into lipolytic enzymes and lipolytic enzyme genes - Google Patents
Method of generating diversity into lipolytic enzymes and lipolytic enzyme genes Download PDFInfo
- Publication number
- WO2002066622A3 WO2002066622A3 PCT/DK2002/000124 DK0200124W WO02066622A3 WO 2002066622 A3 WO2002066622 A3 WO 2002066622A3 DK 0200124 W DK0200124 W DK 0200124W WO 02066622 A3 WO02066622 A3 WO 02066622A3
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- lipolytic
- lipolytic enzyme
- enzymes
- genes
- lipolytic enzymes
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38627—Preparations containing enzymes, e.g. protease or amylase containing lipase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
- C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Detergent Compositions (AREA)
- Cereal-Derived Products (AREA)
Abstract
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
AU2002234513A AU2002234513B2 (en) | 2001-02-23 | 2002-02-25 | Method of generating diversity into lipolytic enzymes and lipolytic enzyme genes |
US10/250,824 US20040101928A1 (en) | 2001-02-23 | 2002-02-25 | Lipolytic enzyme genes |
CA2432375A CA2432375C (en) | 2001-02-23 | 2002-02-25 | Lipolytic enzyme genes |
JP2002566329A JP2004522448A (en) | 2001-02-23 | 2002-02-25 | Lipolytic enzyme gene |
EP02701241A EP1404827A2 (en) | 2001-02-23 | 2002-02-25 | Lipolytic enzyme genes |
US11/474,151 US7271139B2 (en) | 2001-02-23 | 2006-06-23 | Lipolytic enzyme genes |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA200100304 | 2001-02-23 | ||
DKPA200100304 | 2001-02-23 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10250824 A-371-Of-International | 2002-02-25 | ||
US11/474,151 Continuation US7271139B2 (en) | 2001-02-23 | 2006-06-23 | Lipolytic enzyme genes |
Publications (2)
Publication Number | Publication Date |
---|---|
WO2002066622A2 WO2002066622A2 (en) | 2002-08-29 |
WO2002066622A3 true WO2002066622A3 (en) | 2004-01-08 |
Family
ID=8160293
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/DK2002/000124 WO2002066622A2 (en) | 2001-02-23 | 2002-02-25 | Method of generating diversity into lipolytic enzymes and lipolytic enzyme genes |
Country Status (7)
Country | Link |
---|---|
US (2) | US20040101928A1 (en) |
EP (1) | EP1404827A2 (en) |
JP (2) | JP2004522448A (en) |
CN (1) | CN1526013A (en) |
AU (1) | AU2002234513B2 (en) |
CA (1) | CA2432375C (en) |
WO (1) | WO2002066622A2 (en) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
USRE43135E1 (en) | 2001-05-18 | 2012-01-24 | Danisco A/S | Method of improving dough and bread quality |
USRE43341E1 (en) | 1995-06-07 | 2012-05-01 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
US8889371B2 (en) | 2004-07-16 | 2014-11-18 | Dupont Nutrition Biosciences Aps | Lipolytic enzyme: uses thereof in the food industry |
Families Citing this family (30)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7745599B1 (en) | 1995-06-07 | 2010-06-29 | Danisco A/S | Hexose oxidase-encoding DNAs and methods of use thereof |
US8178090B2 (en) | 1995-06-07 | 2012-05-15 | Danisco A/S | Recombinant hexose oxidase |
GB0112226D0 (en) | 2001-05-18 | 2001-07-11 | Danisco | Method of improving dough and bread quality |
DE69623473T3 (en) | 1995-06-07 | 2009-04-23 | Danisco A/S | RECOMBINANT HEXOSE OXIDASE, PROCESS FOR THEIR PREPARATION AND USE |
CA2224203C (en) | 1995-06-07 | 2003-08-12 | Jorn Borch Soe | A method for improving the properties of a flour dough, a flour dough improving composition and improved food products |
EP1803353A3 (en) * | 2001-05-18 | 2007-07-18 | Danisco A/S | Method of preparing a dough with an enzyme |
WO2004031378A2 (en) | 2002-10-01 | 2004-04-15 | Novozymes A/S | Family gh 61 polypeptides |
WO2005001064A2 (en) | 2003-06-25 | 2005-01-06 | Novozymes A/S | Polypeptides having alpha-amylase activity and polypeptides encoding same |
DK1926753T3 (en) | 2004-12-22 | 2011-03-14 | Novozymes As | Hybrid enzymes consisting of an endoamylase as first amino acid sequence and a carbohydrate binding module as second amino acid sequence |
US20080138856A1 (en) * | 2005-02-10 | 2008-06-12 | Novozymes A/S | Enzymatic Enantioselective Ester or Amide Hydrolysis or Synthesis |
WO2008008950A2 (en) * | 2006-07-14 | 2008-01-17 | Novozymes, Inc. | Methods for producing secreted polypeptides having biological activity |
DK2044188T3 (en) * | 2006-07-14 | 2015-12-14 | Novozymes As | Polypeptides with lipase activity and polynucleotides encoding them |
DE102006046857A1 (en) | 2006-10-02 | 2008-04-03 | Ab Enzymes Gmbh | DNA sequence coding for an Aspergillus fumigatus lysophospholipase, e.g. useful for improving the filtration of sugar syrups |
DE102006046719A1 (en) | 2006-10-02 | 2008-04-03 | Ab Enzymes Gmbh | DNA sequence coding for an Aspergillus fumigatus phospholipase, e.g. useful for desliming vegetable oil |
US8956853B2 (en) * | 2007-01-30 | 2015-02-17 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases |
US8460905B2 (en) * | 2007-09-11 | 2013-06-11 | Bunge Oils, Inc. | Enzymatic degumming utilizing a mixture of PLA and PLC phospholipases with reduced reaction time |
US20100112663A1 (en) | 2007-04-09 | 2010-05-06 | Novozymes A/S | Enzymatic Treatment of Skin and Hide Degreasing |
US8241876B2 (en) | 2008-01-07 | 2012-08-14 | Bunge Oils, Inc. | Generation of triacylglycerols from gums |
BR112012018822A2 (en) | 2009-12-21 | 2019-09-24 | Danisco Us Inc | surfactants that optimize the cleaning of lipase-treated lipase-treated stains |
US8765425B2 (en) | 2011-03-23 | 2014-07-01 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
US8759044B2 (en) | 2011-03-23 | 2014-06-24 | Butamax Advanced Biofuels Llc | In situ expression of lipase for enzymatic production of alcohol esters during fermentation |
JP6216495B2 (en) * | 2011-04-19 | 2017-10-18 | 三菱ケミカルフーズ株式会社 | Production method of puffed food |
US9854697B2 (en) * | 2016-05-11 | 2017-12-26 | Facebook, Inc. | Modular network switches, associated structures, and associated methods of manufacture and use |
AU2016432443B2 (en) * | 2016-12-14 | 2024-04-18 | Stichting Voor De Technische Wetenschappen | Thermostable Cas9 nucleases |
CN106676084B (en) * | 2017-02-09 | 2019-11-29 | 浙江工业大学 | A kind of lipase mutant, encoding gene and its application from thermophilic ankle section bacterium |
CN112175967B (en) * | 2020-10-10 | 2021-10-29 | 安徽农业大学 | PEN1 gene for enhancing plant resistance to lepidoptera pests and application thereof |
CN112175976B (en) * | 2020-11-12 | 2021-12-28 | 武汉轻工大学 | High-temperature-resistant lipase gene tllgold and application thereof |
JPWO2023032952A1 (en) * | 2021-08-30 | 2023-03-09 | ||
WO2023203080A1 (en) | 2022-04-20 | 2023-10-26 | Novozymes A/S | Process for producing free fatty acids |
WO2023225459A2 (en) | 2022-05-14 | 2023-11-23 | Novozymes A/S | Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1992019726A1 (en) * | 1991-05-01 | 1992-11-12 | Novo Nordisk A/S | Stabilized enzymes |
WO1995022615A1 (en) * | 1994-02-22 | 1995-08-24 | Novo Nordisk A/S | A method of preparing a variant of a lipolytic enzyme |
WO1997007206A1 (en) * | 1995-08-11 | 1997-02-27 | Okkels, Jens, Sigurd | Method for preparing polypeptide variants |
WO1998041622A1 (en) * | 1997-03-18 | 1998-09-24 | Novo Nordisk A/S | Method for constructing a library using dna shuffling |
US5869438A (en) * | 1990-09-13 | 1999-02-09 | Novo Nordisk A/S | Lipase variants |
WO2000032758A1 (en) * | 1998-11-27 | 2000-06-08 | Novozymes A/S | Lipolytic enzyme variants |
US6159687A (en) * | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5584397A (en) * | 1978-12-20 | 1980-06-25 | Ajinomoto Kk | Fat and oil ester exchange using lipase |
DE3750450T2 (en) | 1986-08-29 | 1995-01-05 | Novo Industri As | Enzyme-based detergent additive. |
DK0575133T4 (en) | 1992-06-16 | 2008-10-20 | Sankyo Lifetech Company Ltd | Hitherto known Phospholipase A1, process for its preparation and its use |
WO1998044804A1 (en) | 1997-04-09 | 1998-10-15 | Danisco A/S | Improved method for preparing flour doughs and products made from such doughs using glycerol oxidase |
JP4307549B2 (en) | 1995-07-14 | 2009-08-05 | ノボザイムス アクティーゼルスカブ | Modified enzyme with lipolytic activity |
DE19701348A1 (en) | 1997-01-16 | 1998-07-23 | Roehm Gmbh | Protein with phospholipase activity |
JP4287149B2 (en) * | 2001-02-07 | 2009-07-01 | ノボザイムス アクティーゼルスカブ | Lipase mutant |
-
2002
- 2002-02-25 US US10/250,824 patent/US20040101928A1/en not_active Abandoned
- 2002-02-25 AU AU2002234513A patent/AU2002234513B2/en not_active Ceased
- 2002-02-25 CA CA2432375A patent/CA2432375C/en not_active Expired - Fee Related
- 2002-02-25 WO PCT/DK2002/000124 patent/WO2002066622A2/en not_active Application Discontinuation
- 2002-02-25 JP JP2002566329A patent/JP2004522448A/en not_active Withdrawn
- 2002-02-25 EP EP02701241A patent/EP1404827A2/en not_active Withdrawn
- 2002-02-25 CN CNA028054725A patent/CN1526013A/en active Pending
-
2006
- 2006-06-23 US US11/474,151 patent/US7271139B2/en not_active Expired - Fee Related
-
2007
- 2007-08-28 JP JP2007221636A patent/JP4130693B2/en not_active Expired - Fee Related
Patent Citations (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5869438A (en) * | 1990-09-13 | 1999-02-09 | Novo Nordisk A/S | Lipase variants |
WO1992019726A1 (en) * | 1991-05-01 | 1992-11-12 | Novo Nordisk A/S | Stabilized enzymes |
WO1995022615A1 (en) * | 1994-02-22 | 1995-08-24 | Novo Nordisk A/S | A method of preparing a variant of a lipolytic enzyme |
US5976855A (en) * | 1994-02-22 | 1999-11-02 | Novo Nordisk A/S | Method of preparing a variant of a lipolytic enzyme |
WO1997007206A1 (en) * | 1995-08-11 | 1997-02-27 | Okkels, Jens, Sigurd | Method for preparing polypeptide variants |
WO1997007205A1 (en) * | 1995-08-11 | 1997-02-27 | Novo Nordisk A/S | Method for preparing polypeptide variants |
WO1998041622A1 (en) * | 1997-03-18 | 1998-09-24 | Novo Nordisk A/S | Method for constructing a library using dna shuffling |
US6159687A (en) * | 1997-03-18 | 2000-12-12 | Novo Nordisk A/S | Methods for generating recombined polynucleotides |
WO2000032758A1 (en) * | 1998-11-27 | 2000-06-08 | Novozymes A/S | Lipolytic enzyme variants |
Non-Patent Citations (11)
Title |
---|
CANADIAN JOURNAL OF BOTANY, vol. 56, no. 16, 1978, EN, pages 1840 - 1843, ISSN: 0008-4026 * |
CRAMERI A ET AL: "DNA shuffling of a family of genes from diverse species accelerates directed evolution", NATURE, MACMILLAN JOURNALS LTD. LONDON, GB, vol. 391, 15 January 1998 (1998-01-15), pages 288 - 291, XP002178865, ISSN: 0028-0836 * |
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; 1978, OSO B A: "THE LIPASE ACTIVITY OF TALAROMYCES-EMERSONII", XP002958623, Database accession no. PREV197967018106 * |
DATABASE BIOSIS [online] BIOSCIENCES INFORMATION SERVICE, PHILADELPHIA, PA, US; December 1996 (1996-12-01), ADEKUNLE A A ET AL: "Lipase activity of fourteen fungi on cucumeropsis mannii seeds.", XP002958624, Database accession no. PREV199900188922 * |
DATABASE EBI [online] XP002958621, retrieved from GPOP Database accession no. AAE05236 * |
DATABASE EBI [online] XP002958622, retrieved from GSP Database accession no. AAR81990 * |
DATABASE EMBL [online] 6 April 1998 (1998-04-06), MULLER S ET AL, XP002958618, retrieved from EMBL Database accession no. AF054513 * |
DATABASE EMBL [online] XP002958619, retrieved from EMBL Database accession no. A32008 * |
DATABASE EMBL [online] XP002958620, retrieved from EMBL Database accession no. AR083396 * |
KIM MYUNG HEE ET AL: "Substitution of glycine 275 by glutamate (G275E) in lipase of Bacillus Stearothermophilus affects its catalytic activity and enantio-and chain length specificity.", JOURNAL OF MICROBIOLOGY AND BIOTECNOLOGY, vol. 10, no. 5, 2000, pages 764 - 769, XP002902706 * |
NIGERIAN JOURNAL OF BOTANY, vol. 9-10, December 1996 (1996-12-01), pages 35 - 40, ISSN: 0795-0128 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
USRE43341E1 (en) | 1995-06-07 | 2012-05-01 | Danisco A/S | Method of improving the properties of a flour dough, a flour dough improving composition and improved food products |
USRE43135E1 (en) | 2001-05-18 | 2012-01-24 | Danisco A/S | Method of improving dough and bread quality |
US8889371B2 (en) | 2004-07-16 | 2014-11-18 | Dupont Nutrition Biosciences Aps | Lipolytic enzyme: uses thereof in the food industry |
Also Published As
Publication number | Publication date |
---|---|
WO2002066622A2 (en) | 2002-08-29 |
US20070010418A1 (en) | 2007-01-11 |
CA2432375A1 (en) | 2002-08-29 |
JP2008035867A (en) | 2008-02-21 |
AU2002234513B2 (en) | 2006-12-07 |
JP4130693B2 (en) | 2008-08-06 |
US20040101928A1 (en) | 2004-05-27 |
US7271139B2 (en) | 2007-09-18 |
CN1526013A (en) | 2004-09-01 |
CA2432375C (en) | 2015-09-08 |
EP1404827A2 (en) | 2004-04-07 |
JP2004522448A (en) | 2004-07-29 |
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