WO2002064802A3 - Regulatory genes suitable for use in gene expression - Google Patents

Regulatory genes suitable for use in gene expression Download PDF

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Publication number
WO2002064802A3
WO2002064802A3 PCT/GB2002/000633 GB0200633W WO02064802A3 WO 2002064802 A3 WO2002064802 A3 WO 2002064802A3 GB 0200633 W GB0200633 W GB 0200633W WO 02064802 A3 WO02064802 A3 WO 02064802A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene expression
regulatory genes
genes suitable
regulatory elements
elements
Prior art date
Application number
PCT/GB2002/000633
Other languages
French (fr)
Other versions
WO2002064802A2 (en
Inventor
Andrew James Greenland
Ian Jepson
Alberto Martinez
Cox Lynne Marie Roxbee
Original Assignee
Syngenta Ltd
Andrew James Greenland
Ian Jepson
Alberto Martinez
Cox Lynne Marie Roxbee
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0103521A external-priority patent/GB0103521D0/en
Priority claimed from GB0103524A external-priority patent/GB0103524D0/en
Priority claimed from GB0103523A external-priority patent/GB0103523D0/en
Priority claimed from GB0103520A external-priority patent/GB0103520D0/en
Application filed by Syngenta Ltd, Andrew James Greenland, Ian Jepson, Alberto Martinez, Cox Lynne Marie Roxbee filed Critical Syngenta Ltd
Priority to CA002433079A priority Critical patent/CA2433079A1/en
Priority to US10/468,250 priority patent/US20040157287A1/en
Priority to EP02703693A priority patent/EP1362111A2/en
Priority to JP2002565114A priority patent/JP2004535774A/en
Publication of WO2002064802A2 publication Critical patent/WO2002064802A2/en
Publication of WO2002064802A3 publication Critical patent/WO2002064802A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8217—Gene switch
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C12N15/8238—Externally regulated expression systems chemically inducible, e.g. tetracycline

Abstract

The present invention relates to novel AlcR regulatory elements and nucleic acid sequences coding therefor, and their use in controlling gene expression in organisms such as plants. DNA constructs containing such nucleic acids, in particular, expression cassettes comprising inducible promoter elements and regulatory elements of the invention, which are capable of acting as 'gene switches', form further aspects of the invention.
PCT/GB2002/000633 2001-02-13 2002-02-13 Regulatory genes suitable for use in gene expression WO2002064802A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CA002433079A CA2433079A1 (en) 2001-02-13 2002-02-13 Regulatory genes suitable for use in gene expression
US10/468,250 US20040157287A1 (en) 2001-02-13 2002-02-13 Regulatory elements suitable for use in gene expression
EP02703693A EP1362111A2 (en) 2001-02-13 2002-02-13 Regulatory genes suitable for use in gene expression
JP2002565114A JP2004535774A (en) 2001-02-13 2002-02-13 Control elements suitable for use in gene expression

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GB0103521A GB0103521D0 (en) 2001-02-13 2001-02-13 Regulatory elements
GB0103521.1 2001-02-13
GB0103524A GB0103524D0 (en) 2001-02-13 2001-02-13 Regulatory elements
GB0103523.7 2001-02-13
GB0103524.5 2001-02-13
GB0103523A GB0103523D0 (en) 2001-02-13 2001-02-13 Regulatory elements
GB0103520.3 2001-02-13
GB0103520A GB0103520D0 (en) 2001-02-13 2001-02-13 Regulatory elements

Publications (2)

Publication Number Publication Date
WO2002064802A2 WO2002064802A2 (en) 2002-08-22
WO2002064802A3 true WO2002064802A3 (en) 2002-12-05

Family

ID=27447923

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2002/000633 WO2002064802A2 (en) 2001-02-13 2002-02-13 Regulatory genes suitable for use in gene expression

Country Status (6)

Country Link
US (1) US20040157287A1 (en)
EP (1) EP1362111A2 (en)
JP (1) JP2004535774A (en)
AR (1) AR032686A1 (en)
CA (1) CA2433079A1 (en)
WO (1) WO2002064802A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4901372B2 (en) * 2006-08-31 2012-03-21 月桂冠株式会社 Deferifericlysin high production mutant, liquid medium for siderophore production, and method for producing siderophore
EP2100961A1 (en) 2008-03-04 2009-09-16 Icon Genetics GmbH Method of protease production in plants
ES2326022B1 (en) 2008-03-25 2010-06-07 Neuron Biopharma, S.A. IMPROVED PROCEDURE FOR BIODIESEL PRODUCTION.
MX2013007621A (en) * 2011-01-18 2013-10-03 Syngenta Participations Ag Methods and compositions for modified ethanol inducible promoter systems.
US11340216B2 (en) 2016-09-13 2022-05-24 Dana-Farber Cancer Institute, Inc. Methods and compositions for the positive selection of protein destabilizers

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021334A1 (en) * 1992-04-13 1993-10-28 Zeneca Limited Dna constructs and plants incorporating them
WO1994013820A1 (en) * 1992-12-10 1994-06-23 Gist-Brocades N.V. Production of heterologous proteins in filamentous fungi
WO2000044917A1 (en) * 1999-02-01 2000-08-03 Syngenta Limited Inducible expression system for use in plants

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198345A (en) * 1985-04-15 1993-03-30 Gist-Brocades N.V. Vectors in use in filamentous fungi

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993021334A1 (en) * 1992-04-13 1993-10-28 Zeneca Limited Dna constructs and plants incorporating them
WO1994013820A1 (en) * 1992-12-10 1994-06-23 Gist-Brocades N.V. Production of heterologous proteins in filamentous fungi
WO2000044917A1 (en) * 1999-02-01 2000-08-03 Syngenta Limited Inducible expression system for use in plants

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
EHRLICH K C ET AL: "Binding of the C6-zinc cluster protein, AFLR, to the promoters of aflatoxin pathway biosynthesis genes in Aspergillus parasiticus.", GENE (AMSTERDAM), vol. 230, no. 2, 16 April 1999 (1999-04-16), pages 249 - 257, XP002209396, ISSN: 0378-1119 *
NIKOLAEV I ET AL: "A SINGLE AMINO ACID, OUTSIDE THE ALCR ZINC BINUCLEAR CLUSTER, IS INVOLVED IN DNA BINDING AND IN TRANSCRIPTIONAL REGULATION OF THE ALC GENES IN ASPERGILLUS NIDULANS", MOLECULAR MICROBIOLOGY, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 31, no. 4, February 1999 (1999-02-01), pages 1115 - 1124, XP000974774, ISSN: 0950-382X *
TODD RICHARD B ET AL: "Evolution of a fungal regulatory gene family: The Zn(II)2Cys6 binuclear cluster DNA binding motif.", FUNGAL GENETICS AND BIOLOGY, vol. 21, no. 3, 1997, pages 388 - 405, XP002209397, ISSN: 1087-1845 *

Also Published As

Publication number Publication date
WO2002064802A2 (en) 2002-08-22
US20040157287A1 (en) 2004-08-12
CA2433079A1 (en) 2002-08-22
JP2004535774A (en) 2004-12-02
AR032686A1 (en) 2003-11-19
EP1362111A2 (en) 2003-11-19

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