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Rolling circle amplification detection of rna and dna

Info

Publication number
WO2002050310A3
WO2002050310A3 PCT/US2001/049780 US0149780W WO2002050310A3 WO 2002050310 A3 WO2002050310 A3 WO 2002050310A3 US 0149780 W US0149780 W US 0149780W WO 2002050310 A3 WO2002050310 A3 WO 2002050310A3
Authority
WO
Grant status
Application
Patent type
Prior art keywords
rca
circle
rolling
amplification
cells
Prior art date
Application number
PCT/US2001/049780
Other languages
French (fr)
Other versions
WO2002050310A9 (en )
WO2002050310A2 (en )
Inventor
Cristina M Attix
Allen T Christian
Melissa S Pattee
James D Tucker
Original Assignee
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6802General aspects
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for PCR assay

Abstract

Rolling circle amplification (RCA) has been useful for detecting point mutations in isolated nucleic acids, but its application in cytological preparations has been problematic. By pretreating cells with a combination of restriction enzymes and exonucleases, we demonstrate RCA in solution and in situ to detect gene copy number and single base mutations. It can also detect and quantify transcribed RNA in individual cells, making it a versatile tool for cell-based assays.
PCT/US2001/049780 2000-12-20 2001-12-18 Rolling circle amplification detection of rna and dna WO2002050310A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US25721600 true 2000-12-20 2000-12-20
US60/257,216 2000-12-20

Publications (3)

Publication Number Publication Date
WO2002050310A2 true WO2002050310A2 (en) 2002-06-27
WO2002050310A9 true WO2002050310A9 (en) 2002-11-28
WO2002050310A3 true true WO2002050310A3 (en) 2004-03-04

Family

ID=22975349

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/049780 WO2002050310A3 (en) 2000-12-20 2001-12-18 Rolling circle amplification detection of rna and dna

Country Status (2)

Country Link
US (1) US6783943B2 (en)
WO (1) WO2002050310A3 (en)

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US7141371B2 (en) * 2002-09-06 2006-11-28 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for detecting and localizing DNA mutations by microarray
CN101213310A (en) 2005-04-12 2008-07-02 Novia公司名下的现场Rcp公司 Methods for production of oligonucleotides
CN101238221B (en) 2005-04-12 2011-11-16 Novia公司名下的现场Rcp公司 Ring probe and its uses in identifying biomolecule
US7449297B2 (en) 2005-04-14 2008-11-11 Euclid Diagnostics Llc Methods of copying the methylation pattern of DNA during isothermal amplification and microarrays
WO2006122215A3 (en) * 2005-05-10 2007-03-22 Eric A Johnson Methods of mapping polymorphisms and polymorphism microarrays
US7960104B2 (en) * 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
EP2620510B1 (en) * 2005-06-15 2016-10-12 Complete Genomics Inc. Single molecule arrays for genetic and chemical analysis
US20070168197A1 (en) * 2006-01-18 2007-07-19 Nokia Corporation Audio coding
US20090264299A1 (en) * 2006-02-24 2009-10-22 Complete Genomics, Inc. High throughput genome sequencing on DNA arrays
JP5180845B2 (en) 2006-02-24 2013-04-10 カリダ・ジェノミックス・インコーポレイテッドCallida Genomics, Inc. High throughput genome sequencing on Dna array
GB0621361D0 (en) * 2006-10-26 2006-12-06 Fermentas Uab Use of DNA polymerases
US8518640B2 (en) * 2007-10-29 2013-08-27 Complete Genomics, Inc. Nucleic acid sequencing and process
US7910302B2 (en) 2006-10-27 2011-03-22 Complete Genomics, Inc. Efficient arrays of amplified polynucleotides
US20090105961A1 (en) * 2006-11-09 2009-04-23 Complete Genomics, Inc. Methods of nucleic acid identification in large-scale sequencing
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US7901890B2 (en) * 2007-11-05 2011-03-08 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors employing selective methylation
US8592150B2 (en) 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US8415099B2 (en) 2007-11-05 2013-04-09 Complete Genomics, Inc. Efficient base determination in sequencing reactions
US7897344B2 (en) * 2007-11-06 2011-03-01 Complete Genomics, Inc. Methods and oligonucleotide designs for insertion of multiple adaptors into library constructs
US8298768B2 (en) 2007-11-29 2012-10-30 Complete Genomics, Inc. Efficient shotgun sequencing methods
US9524369B2 (en) 2009-06-15 2016-12-20 Complete Genomics, Inc. Processing and analysis of complex nucleic acid sequence data
US20090263872A1 (en) * 2008-01-23 2009-10-22 Complete Genomics Inc. Methods and compositions for preventing bias in amplification and sequencing reactions
US8617811B2 (en) * 2008-01-28 2013-12-31 Complete Genomics, Inc. Methods and compositions for efficient base calling in sequencing reactions
WO2009132028A1 (en) * 2008-04-21 2009-10-29 Complete Genomics, Inc. Array structures for nucleic acid detection
CA2827497C (en) * 2011-02-15 2014-12-02 Leica Biosystems Newcastle Ltd. Method for localized in situ detection of mrna
WO2013010074A1 (en) 2011-07-13 2013-01-17 Primeradx, Inc. Multimodal methods for simultaneous detection and quantification of multiple nucleic acids in a sample
US20130157259A1 (en) * 2011-12-15 2013-06-20 Samsung Electronics Co., Ltd. Method of amplifying dna from rna in sample and use thereof

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EP0736769A1 (en) * 1992-12-22 1996-10-09 Merck Frosst Canada Inc. An assay for determining cyclooxygenase-1 activity
WO1997019193A2 (en) * 1995-11-21 1997-05-29 Yale University Unimolecular segment amplification and detection
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Title
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Also Published As

Publication number Publication date Type
WO2002050310A9 (en) 2002-11-28 application
WO2002050310A2 (en) 2002-06-27 application
US20020177142A1 (en) 2002-11-28 application
US6783943B2 (en) 2004-08-31 grant

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