WO2002040690A3 - Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways - Google Patents

Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways Download PDF

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Publication number
WO2002040690A3
WO2002040690A3 PCT/US2001/043686 US0143686W WO0240690A3 WO 2002040690 A3 WO2002040690 A3 WO 2002040690A3 US 0143686 W US0143686 W US 0143686W WO 0240690 A3 WO0240690 A3 WO 0240690A3
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WO
WIPO (PCT)
Prior art keywords
coa synthetase
acyl coa
fatty acid
chain length
medium chain
Prior art date
Application number
PCT/US2001/043686
Other languages
French (fr)
Other versions
WO2002040690A2 (en
WO2002040690A9 (en
Inventor
Stephanie Aquin
Oliver P Peoples
Kristi D Snell
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Metabolix Inc
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Application filed by Metabolix Inc filed Critical Metabolix Inc
Priority to DE60141594T priority Critical patent/DE60141594D1/en
Priority to CA2428713A priority patent/CA2428713C/en
Priority to JP2002543002A priority patent/JP4024676B2/en
Priority to AU2002236464A priority patent/AU2002236464B2/en
Priority to AU3646402A priority patent/AU3646402A/en
Priority to EP01985993A priority patent/EP1334181B1/en
Priority to AT01985993T priority patent/ATE461273T1/en
Publication of WO2002040690A2 publication Critical patent/WO2002040690A2/en
Publication of WO2002040690A3 publication Critical patent/WO2002040690A3/en
Publication of WO2002040690A9 publication Critical patent/WO2002040690A9/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Biological Depolymerization Polymers (AREA)

Abstract

Methods for producing polyhydroxyalkanoates (PHAs) from fatty acid biosynthetic pathways using a 3-hydroxy acyl ACP thioesterase, a PHA synthase, and an acyl CoA synthetase, have been developed. Methodology for enabling PHA production from fatty acid biosynthetic pathways in non-native bacterial PHA producers and plants using an enzyme having the catalytic activity of 3-hydroxy acyl ACP thioesterase, an acyl CoA synthetase with substrate specificity for medium chain length 3-hydroxy fatty acids, and a medium chain length PHA synthase, has been developed. Acyl CoA synthetase activity can be supplied either by the endogenous acyl CoA synthetase of the host organism, when sufficiently expressed, or the host organism's activity can be supplemented by the expression of a recombinant acyl CoA synthetase gene. New strategies are described for plant based PHA production in the chloroplasts, cytosol, and peroxisomes of biomass crops as well as the plastids, cytosol, and peroxisomes of oil seed crops.
PCT/US2001/043686 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways WO2002040690A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DE60141594T DE60141594D1 (en) 2000-11-17 2001-11-16 PREPARATION OF POLYHYDROXYALKANOATES MEDIUM CHAIN LENGTH FROM FATTY ACID BIOSYNTHESIS
CA2428713A CA2428713C (en) 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
JP2002543002A JP4024676B2 (en) 2000-11-17 2001-11-16 Production of intermediate chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
AU2002236464A AU2002236464B2 (en) 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
AU3646402A AU3646402A (en) 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
EP01985993A EP1334181B1 (en) 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways
AT01985993T ATE461273T1 (en) 2000-11-17 2001-11-16 PRODUCTION OF MEDIUM CHAIN POLYHYDROXYAL CANOATES FROM FATTY ACID BIOSYNTHESIS PATHWAYS

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24953500P 2000-11-17 2000-11-17
US60/249,535 2000-11-17

Publications (3)

Publication Number Publication Date
WO2002040690A2 WO2002040690A2 (en) 2002-05-23
WO2002040690A3 true WO2002040690A3 (en) 2003-05-22
WO2002040690A9 WO2002040690A9 (en) 2003-11-20

Family

ID=22943899

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/043686 WO2002040690A2 (en) 2000-11-17 2001-11-16 Production of medium chain length polyhydroxyalkanoates from fatty acid biosynthetic pathways

Country Status (9)

Country Link
US (1) US7786355B2 (en)
EP (1) EP1334181B1 (en)
JP (4) JP4024676B2 (en)
AT (1) ATE461273T1 (en)
AU (2) AU2002236464B2 (en)
CA (1) CA2428713C (en)
DE (1) DE60141594D1 (en)
ES (1) ES2340915T3 (en)
WO (1) WO2002040690A2 (en)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69938105T2 (en) 1998-08-04 2009-01-29 Metabolix, Inc., Cambridge PREPARATION OF POLYHYDROXYALKANOATES FROM POLYOLES
EP1654373B1 (en) 2002-05-10 2012-08-15 Metabolix, Inc. Bioabsorbable polymer containing 2-hydroxyacid monomers
DE10240035A1 (en) 2002-08-30 2004-03-11 Rehm, Bernd H.A., PD Dr.rer.nat. Biogenic polyester particles of a defined size with functionalized surfaces: manufacturing processes and pharmaceutical preparations containing them
EP1863916B1 (en) * 2005-03-16 2011-08-24 Metabolix, Inc. Chemically inducible expression of biosynthetic pathways
KR100979694B1 (en) * 2005-05-24 2010-09-02 한국과학기술원 Cells or Plants Having an Producing Ability of Polylactate or Its Copolymers and Method for Preparing Polylactate or Its Copolymers Using the Same
EP1752532A1 (en) * 2005-08-09 2007-02-14 Helmholtz-Zentrum für Infektionsforschung GmbH Extracellular polyhydroxyalkanoates produced by genetically engineered microorganisms
JP2009509530A (en) * 2005-09-27 2009-03-12 ヘルムート アダム レフム，ベルン Polymer particles and uses thereof
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
WO2008008008A1 (en) * 2006-07-13 2008-01-17 St. Jude Medical Ab Information management in devices worn by a use
CA3178593A1 (en) * 2007-03-28 2008-10-02 Genomatica, Inc. Enhanced production of fatty acid derivatives
MX2010012192A (en) 2008-05-16 2011-04-26 Ls9 Inc Methods and compositions for producing hydrocarbons.
WO2010062480A2 (en) 2008-10-28 2010-06-03 Ls9, Inc. Methods and compositions for producing fatty alcohols
CN102317437B (en) 2008-12-12 2013-08-21 麦特波力克斯公司 Green process and compositions for producing poly(5hv) and 5 carbon chemicals
WO2010102217A1 (en) 2009-03-05 2010-09-10 Metabolix, Inc. Stable, fertile, high polyhydroxyalkanoate producing plants and methods of producing them
US20100229256A1 (en) 2009-03-05 2010-09-09 Metabolix, Inc. Propagation of transgenic plants
WO2010126891A1 (en) 2009-04-27 2010-11-04 Ls9, Inc. Production of fatty acid esters
CZ304183B6 (en) 2012-08-27 2013-12-11 Vysoké ucení technické v Brne Process for preparing polyhydroxyalkanoates (PHA) on oil substrate
US20140073022A1 (en) 2012-09-10 2014-03-13 Wisconsin Alumni Research Foundation Production of polyhydroxyalkanoates with a defined composition from an unrelated carbon source
US9708630B1 (en) 2013-10-08 2017-07-18 Wisconsin Alumni Research Foundation Cells and methods for producing fatty alcohols
JP6779652B2 (en) 2016-04-20 2020-11-04 花王株式会社 Lipid production method
WO2020251932A1 (en) * 2019-06-10 2020-12-17 Yield10 Bioscience, Inc. Transgenic land plants that express a polyhydroxyalkanoate synthase seed specifically with cytosolic localization
EP4153765A1 (en) 2020-05-20 2023-03-29 Wisconsin Alumni Research Foundation Cells and methods for producing methyl ketones
WO2024072953A1 (en) * 2022-09-30 2024-04-04 Kimberly-Clark Worldwide, Inc. In vitro bioproduction of specific chain length poly(hydroxyalkanoate) monomers

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006854A1 (en) * 1996-08-13 1998-02-19 Monsanto Company Dna sequence useful for the production of polyhydroxyalkanoates

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5034322A (en) * 1983-01-17 1991-07-23 Monsanto Company Chimeric genes suitable for expression in plant cells
US5352605A (en) * 1983-01-17 1994-10-04 Monsanto Company Chimeric genes for transforming plant cells using viral promoters
US5668298A (en) * 1984-12-24 1997-09-16 Eli Lilly And Company Selectable marker for development of vectors and transformation systems in plants
US5420034A (en) * 1986-07-31 1995-05-30 Calgene, Inc. Seed-specific transcriptional regulation
US5276268A (en) * 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5268463A (en) * 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5250430A (en) * 1987-06-29 1993-10-05 Massachusetts Institute Of Technology Polyhydroxyalkanoate polymerase
US5245023A (en) * 1987-06-29 1993-09-14 Massachusetts Institute Of Technology Method for producing novel polyester biopolymers
US5614395A (en) * 1988-03-08 1997-03-25 Ciba-Geigy Corporation Chemically regulatable and anti-pathogenic DNA sequences and uses thereof
EP0463056A1 (en) * 1989-03-17 1992-01-02 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5302523A (en) * 1989-06-21 1994-04-12 Zeneca Limited Transformation of plant cells
EP0482077B1 (en) 1989-07-10 1998-10-21 Massachusetts Institute Of Technology Method for producing polyester biopolymers
WO1993020216A1 (en) 1991-02-22 1993-10-14 University Technologies International, Inc. Oil-body protein cis-elements as regulatory signals
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
GB9304200D0 (en) * 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
DK152291D0 (en) 1991-08-28 1991-08-28 Danisco PROCEDURE AND CHEMICAL RELATIONS
EP0604662B1 (en) * 1992-07-07 2008-06-18 Japan Tobacco Inc. Method of transforming monocotyledon
GB9326271D0 (en) * 1993-12-23 1994-02-23 Zeneca Ltd Expression of self-processing polyprotein in transgenic plants
DE4433134A1 (en) * 1994-09-16 1996-03-21 Buck Chem Tech Werke Process for the preparation of polyhydroxy fatty acids and recombinant bacterial strains for carrying out the process
US5932440A (en) * 1996-08-16 1999-08-03 Life Technologies, Inc. Mammalian ribonuclease inhibitors and use thereof
FI972293A (en) 1997-05-30 1998-12-01 Joseph Atabekov Methods for co-expression of more than one gene
US6143952A (en) * 1998-03-31 2000-11-07 Regents Of The University Of Minnesota Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase
DE69938105T2 (en) * 1998-08-04 2009-01-29 Metabolix, Inc., Cambridge PREPARATION OF POLYHYDROXYALKANOATES FROM POLYOLES
WO2000055328A1 (en) 1999-03-15 2000-09-21 Universite Laval Method for producing polyhydroxyalkanoates in recombinant organisms
EP1654373B1 (en) 2002-05-10 2012-08-15 Metabolix, Inc. Bioabsorbable polymer containing 2-hydroxyacid monomers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998006854A1 (en) * 1996-08-13 1998-02-19 Monsanto Company Dna sequence useful for the production of polyhydroxyalkanoates

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
FIEDLER SILKE ET AL: "PhaG-mediated synthesis of poly(3-hydroxyalkanoates) consisting of medium-chain-length constituents from nonrelated carbon sources in recombinant Pseudomonas fragi.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 66, no. 5, May 2000 (2000-05-01), pages 2117 - 2124, XP002211661, ISSN: 0099-2240 *
KLINKE STEFAN ET AL: "Production of medium-chain-length poly(3-hydroxyalkanoates) from gluconate by recombinant Escherichia coli.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 2, February 1999 (1999-02-01), pages 540 - 548, XP002211663, ISSN: 0099-2240 *
REHM BERND H A ET AL: "A new metabolic link between fatty acid de novo synthesis and polyhydroxyalkanoic acid synthesis: The phaG gene from Pseudomonas putida KT2440 encodes a 3-hydroxyacyl-acyl carrier protein-coenzyme A transferase.", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 273, no. 37, pages 24044 - 24051, XP002211662, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
WO2002040690A2 (en) 2002-05-23
EP1334181B1 (en) 2010-03-17
US7786355B2 (en) 2010-08-31
EP1334181A2 (en) 2003-08-13
JP2007125043A (en) 2007-05-24
JP2004534507A (en) 2004-11-18
CA2428713A1 (en) 2002-05-23
JP4121541B2 (en) 2008-07-23
US20030017576A1 (en) 2003-01-23
AU2002236464B2 (en) 2005-09-15
DE60141594D1 (en) 2010-04-29
ATE461273T1 (en) 2010-04-15
JP4024676B2 (en) 2007-12-19
JP2008194049A (en) 2008-08-28
ES2340915T3 (en) 2010-06-11
WO2002040690A9 (en) 2003-11-20
CA2428713C (en) 2012-04-17
JP2007222185A (en) 2007-09-06
AU3646402A (en) 2002-05-27

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