WO2002040631A3 - Dipeptide seryl-histidine and related oligopeptides cleave dna, protein, and a carboxyl ester - Google Patents

Dipeptide seryl-histidine and related oligopeptides cleave dna, protein, and a carboxyl ester Download PDF

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Publication number
WO2002040631A3
WO2002040631A3 PCT/US2001/043079 US0143079W WO0240631A3 WO 2002040631 A3 WO2002040631 A3 WO 2002040631A3 US 0143079 W US0143079 W US 0143079W WO 0240631 A3 WO0240631 A3 WO 0240631A3
Authority
WO
WIPO (PCT)
Prior art keywords
seryl
dipeptide
histidine
protein
carboxyl ester
Prior art date
Application number
PCT/US2001/043079
Other languages
French (fr)
Other versions
WO2002040631A2 (en
Inventor
Xiaozhou Chen
Yunsheng Li
Scott Hatfield
Original Assignee
Univ Ohio
Xiaozhou Chen
Yunsheng Li
Scott Hatfield
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Ohio, Xiaozhou Chen, Yunsheng Li, Scott Hatfield filed Critical Univ Ohio
Publication of WO2002040631A2 publication Critical patent/WO2002040631A2/en
Publication of WO2002040631A3 publication Critical patent/WO2002040631A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/08—Tripeptides
    • C07K5/0802—Tripeptides with the first amino acid being neutral
    • C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
    • C07K5/081—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1005—Tetrapeptides with the first amino acid being neutral and aliphatic
    • C07K5/1013—Tetrapeptides with the first amino acid being neutral and aliphatic the side chain containing O or S as heteroatoms, e.g. Cys, Ser
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C07K7/04—Linear peptides containing only normal peptide links
    • C07K7/08—Linear peptides containing only normal peptide links having 12 to 20 amino acids

Abstract

A compound other than Ser- His, having the structure (Ser/Cys) - Xaam - His - Xaan (EM'), where 0<=(m+n)<=12, is useful as a nuclease or protease. A compound having the structure EM-L-BM, where EM is EM' above or Ser-His, L is a linker and BM is a nucleotide or amino acid sequence binding moiety, is also useful as a nuclease or protease. The compounds, EM and EM-L-BM which act as nucleases are useful in labeling a nucleic acid by nick translation.
PCT/US2001/043079 2000-11-14 2001-11-14 Dipeptide seryl-histidine and related oligopeptides cleave dna, protein, and a carboxyl ester WO2002040631A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US24796900P 2000-11-14 2000-11-14
US60/247,969 2000-11-14

Publications (2)

Publication Number Publication Date
WO2002040631A2 WO2002040631A2 (en) 2002-05-23
WO2002040631A3 true WO2002040631A3 (en) 2003-06-19

Family

ID=22937100

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/043079 WO2002040631A2 (en) 2000-11-14 2001-11-14 Dipeptide seryl-histidine and related oligopeptides cleave dna, protein, and a carboxyl ester

Country Status (1)

Country Link
WO (1) WO2002040631A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012166647A1 (en) * 2011-05-27 2012-12-06 Life Technologies Corporation Methods for manipulating biomolecules
DE102016001407A1 (en) 2016-02-09 2017-08-24 Fresenius Medical Care Deutschland Gmbh Blood treatment with inactivation of free nucleic acids

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11196819A (en) * 1998-01-13 1999-07-27 Mikio Shimizu Use of specific enzyme activity of amino acid, dipeptide and dinucleotide (part 2)

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11196819A (en) * 1998-01-13 1999-07-27 Mikio Shimizu Use of specific enzyme activity of amino acid, dipeptide and dinucleotide (part 2)

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BIOMEDICAL & ENVIRONMENTAL MASS SPECTROMETRY (1989), 18(10), 910-17, 1989 *
DATABASE CA [online] CHEMICAL ABSTRACTS SERVICE, COLUMBUS, OHIO, US; KULIK, WILLEM ET AL: "Fast atom bombardment tandem mass spectrometry for amino acid sequence determination in tripeptides", XP002227765, retrieved from STN Database accession no. 113:6782 CA *
DATABASE WPI 1999 Derwent World Patents Index; AN 1999-471871, XP002227766, "New modified amino acid specific enzyme active useful biochemical catalyst" *
Y LI ET AL.: "Peptide seryl-histidine and related oligopeptides cleave DNA, protein and a carboxyl ester", BIOORGANIC & MEDICINAL CHEMISTRY., vol. 8, no. 12, December 2000 (2000-12-01), ELSEVIER SCIENCE LTD., GB, pages 2675 - 2680, XP002227764, ISSN: 0968-0896 *

Also Published As

Publication number Publication date
WO2002040631A2 (en) 2002-05-23

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