WO2002020150A2 - Photocleavable protecting groups - Google Patents

Photocleavable protecting groups Download PDF

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Publication number
WO2002020150A2
WO2002020150A2 PCT/IB2001/001650 IB0101650W WO0220150A2 WO 2002020150 A2 WO2002020150 A2 WO 2002020150A2 IB 0101650 W IB0101650 W IB 0101650W WO 0220150 A2 WO0220150 A2 WO 0220150A2
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WO
WIPO (PCT)
Prior art keywords
compound
support
method
reactive site
ofthe
Prior art date
Application number
PCT/IB2001/001650
Other languages
English (en)
French (fr)
Other versions
WO2002020150A3 (en
Inventor
Glenn H. Mcgall
Anthony D. Barone
Original Assignee
Affymetrix, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US65959900A priority Critical
Priority to US09/659,599 priority
Application filed by Affymetrix, Inc. filed Critical Affymetrix, Inc.
Publication of WO2002020150A2 publication Critical patent/WO2002020150A2/en
Publication of WO2002020150A3 publication Critical patent/WO2002020150A3/en

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00Nanotechnology for materials or surface science, e.g. nanocomposites
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047Simultaneous synthesis of different peptide species; Peptide libraries
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/062General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for alpha- or omega-carboxy functions
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/063General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for alpha-amino functions
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/064General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for omega-amino or -guanidino functions
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/065General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for hydroxy functions, not being part of carboxy functions
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/066General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for omega-amido functions
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    • B01J2219/00277Apparatus
    • B01J2219/00351Means for dispensing and evacuation of reagents
    • B01J2219/00427Means for dispensing and evacuation of reagents using masks
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    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
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    • B01J2219/00731Saccharides
    • CCHEMISTRY; METALLURGY
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    • C07B2200/11Compounds covalently bound to a solid support
    • CCHEMISTRY; METALLURGY
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    • C40B40/00Libraries per se, e.g. arrays, mixtures
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    • C40B60/00Apparatus specially adapted for use in combinatorial chemistry or with libraries
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of products other than chlorine, adipic acid, caprolactam, or chlorodifluoromethane, e.g. bulk or fine chemicals or pharmaceuticals
    • Y02P20/55Synthetic design, e.g. reducing the use of auxiliary or protecting groups
PCT/IB2001/001650 2000-09-11 2001-09-11 Photocleavable protecting groups WO2002020150A2 (en)

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US65959900A true 2000-09-11 2000-09-11
US09/659,599 2000-09-11

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JP2002524622A JP2005523232A (ja) 2000-09-11 2001-09-11 光切断可能保護基
EP01972369A EP1325017A2 (en) 2000-09-11 2001-09-11 Photocleavable protecting groups
AU9214201A AU9214201A (en) 2000-09-11 2001-09-11 Photocleavable protecting groups
CA002421732A CA2421732A1 (en) 2000-09-11 2001-09-11 Photocleavable protecting groups

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WO2002020150A2 true WO2002020150A2 (en) 2002-03-14
WO2002020150A3 WO2002020150A3 (en) 2003-03-13

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EP (1) EP1325017A2 (enIt)
JP (1) JP2005523232A (enIt)
AU (1) AU9214201A (enIt)
CA (1) CA2421732A1 (enIt)
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Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003035664A2 (en) * 2001-10-25 2003-05-01 Chemogenix Gmbh Method for covalently attaching nucleosides and/or nucleotides on surfaces and method for determining coupling yields in the synthesis of nucleotides
WO2004058393A2 (de) * 2002-12-23 2004-07-15 Febit Biotech Gmbh Verfahren zum validierten aufbau von arrays
WO2004058391A2 (de) * 2002-12-23 2004-07-15 Febit Biotech Gmbh Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren
WO2004074300A2 (en) * 2003-02-21 2004-09-02 Nigu Chemie Gmbh Novel photolabile protective groups for improved processes to prepare oligonucleotide arrays
EP1833987A2 (en) * 2004-12-15 2007-09-19 Yeda Research and Development Co. Ltd. at The Weizmann Institute of Science A single-step platform for on-chip integration of bio-molecules
US8101737B2 (en) 2004-12-31 2012-01-24 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
US8133987B2 (en) 2004-12-31 2012-03-13 Affymetrix, Inc. Primer array synthesis and validation
WO2013050795A1 (en) * 2011-10-03 2013-04-11 Femtonics Kft. Use of photoactive compounds
WO2014150845A1 (en) * 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Photocleavable deoxynucleotides with high-resolution control of deprotection kinetics
US10007182B2 (en) 2016-01-11 2018-06-26 Samsung Electronics Co., Ltd. Photoresist composition and method of manufacturing semiconductor device using the same

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6147205A (en) * 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
US20080312092A1 (en) * 2005-07-08 2008-12-18 The University Of Denver Photoinduced Signal Amplification Through Externally Sensitized Photofragmentation in Masked Photosensitizers
US20090247420A1 (en) * 2005-12-12 2009-10-01 Kutateladze Andrei G Method for encoding and screening combinatorial libraries
US20070224616A1 (en) * 2006-03-24 2007-09-27 Erdogan Gulari Method for forming molecular sequences on surfaces
KR100801080B1 (ko) 2006-08-07 2008-02-05 삼성전자주식회사 광분해성 화합물 및 상기 화합물이 결합된 올리고머 프로브어레이용 기판
WO2009026313A1 (en) * 2007-08-20 2009-02-26 Colorado Seminary, Which Owns And Operates The University Of Denver Photoinduced signal amplification through externally sensitized photofragmentation in masked photosensitizers and photoamplified fluorescence turn-off system
WO2010033844A2 (en) * 2008-09-22 2010-03-25 Biomicro, Inc. Selective processing of biological material on a microarray substrate
CN103382212B (zh) * 2013-07-06 2016-08-31 中国科学院成都生物研究所 5′位改性嘌呤霉素化合物及其制备方法和用途

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010128A1 (en) * 1992-11-02 1994-05-11 Affymax Technologies N.V. Novel photoreactive protecting groups
US5763599A (en) * 1994-12-16 1998-06-09 Wolfgang Pfleiderer Nucleoside derivatives with photolabile protective groups

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2412510A (en) * 1944-06-02 1946-12-10 American Chem Paint Co Methods and compositions for killing weeds
US2577969A (en) * 1944-08-17 1951-12-11 American Chem Paint Co Compositions for killing weeds
BE502441A (enIt) * 1950-04-08
GB1432809A (en) * 1973-04-19 1976-04-22 Beecham Group Ltd Coumarin derivatives
US3879356A (en) * 1973-08-29 1975-04-22 Eastman Kodak Co Light-sensitive polymeric compositions
US3944538A (en) * 1973-10-02 1976-03-16 Miklos Bodanszky Process and apparatus for the synthesis of peptides not linked to polymers
US4242974A (en) * 1980-03-10 1981-01-06 The Singer Company Pattern feed elongation in electronic sewing machine
US5430136A (en) * 1984-10-16 1995-07-04 Chiron Corporation Oligonucleotides having selectably cleavable and/or abasic sites
LU85853A1 (fr) * 1985-04-16 1986-11-05 Oreal Nouveaux nitroaminophenols,leur procede de preparation et leur utilisation en teinture des fibres keratiniques,nouveaux nitroaminobenzenes intermediaires et leur utilisation en teinture des fibres keratiniques
US5700637A (en) * 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
US5200051A (en) * 1988-11-14 1993-04-06 I-Stat Corporation Wholly microfabricated biosensors and process for the manufacture and use thereof
US5212050A (en) * 1988-11-14 1993-05-18 Mier Randall M Method of forming a permselective layer
US5424186A (en) * 1989-06-07 1995-06-13 Affymax Technologies N.V. Very large scale immobilized polymer synthesis
US5143854A (en) * 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5527681A (en) * 1989-06-07 1996-06-18 Affymax Technologies N.V. Immobilized molecular synthesis of systematically substituted compounds
US5744101A (en) * 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5215899A (en) * 1989-11-09 1993-06-01 Miles Inc. Nucleic acid amplification employing ligatable hairpin probe and transcription
US5650489A (en) * 1990-07-02 1997-07-22 The Arizona Board Of Regents Random bio-oligomer library, a method of synthesis thereof, and a method of use thereof
DE69217497D1 (de) * 1991-09-18 1997-03-27 Affymax Tech Nv Verfahren zur synthese der verschiedenen sammlungen von oligomeren
US5639603A (en) * 1991-09-18 1997-06-17 Affymax Technologies N.V. Synthesizing and screening molecular diversity
US5384261A (en) * 1991-11-22 1995-01-24 Affymax Technologies N.V. Very large scale immobilized polymer synthesis using mechanically directed flow paths
DE69233087D1 (de) * 1991-11-22 2003-07-03 Affymetrix Inc N D Ges D Staat Verfahren zur Herstellung von Polymerarrays
US5242974A (en) * 1991-11-22 1993-09-07 Affymax Technologies N.V. Polymer reversal on solid surfaces
DE4207983A1 (de) * 1992-03-13 1993-09-16 Asea Brown Boveri Reaktorschalter
US5420328A (en) * 1992-09-11 1995-05-30 Affymax Technologies, N.V. Methods for the synthesis of phosphonate esters
US5412087A (en) * 1992-04-24 1995-05-02 Affymax Technologies N.V. Spatially-addressable immobilization of oligonucleotides and other biological polymers on surfaces
US5541061A (en) * 1992-04-29 1996-07-30 Affymax Technologies N.V. Methods for screening factorial chemical libraries
JPH08509751A (ja) * 1993-01-21 1996-10-15 オレゴン州 表面の化学的官能化
US5582955A (en) * 1994-06-23 1996-12-10 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Chemical functionalization of surfaces
DE4302459A1 (de) * 1993-01-29 1994-08-04 Bayer Ag Sulfocumarinhaltige Nukleotide und deren Verwendung in Nachweisverfahren für Nukleinsäuren
CA2270132A1 (en) * 1996-11-06 1998-05-14 Sequenom, Inc. Dna diagnostics based on mass spectrometry
DE59407895D1 (de) * 1993-05-12 1999-04-15 Novartis Ag Nukleoside und Oligonukleotide mit 2'-Ethergruppen
JP3250878B2 (ja) * 1993-07-15 2002-01-28 日清紡績株式会社 熱溶融型プリンター用ohpシート
US5503805A (en) * 1993-11-02 1996-04-02 Affymax Technologies N.V. Apparatus and method for parallel coupling reactions
US5679267A (en) * 1994-04-04 1997-10-21 Texas Instruments Incorporated Dual etching of ceramic materials with an elevated thin film
US5623023A (en) * 1995-05-23 1997-04-22 Taiyo Ink Manufacuturing Co., Ltd. Curable compositions which release imidazole upon irradiation
US5830539A (en) * 1995-11-17 1998-11-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Methods for functionalizing and coating substrates and devices made according to the methods
US6022963A (en) * 1995-12-15 2000-02-08 Affymetrix, Inc. Synthesis of oligonucleotide arrays using photocleavable protecting groups
US6147205A (en) * 1995-12-15 2000-11-14 Affymetrix, Inc. Photocleavable protecting groups and methods for their use
US20110028350A1 (en) * 1995-12-15 2011-02-03 Affymetrix, Inc. Photocleavable protecting groups
US6013440A (en) * 1996-03-11 2000-01-11 Affymetrix, Inc. Nucleic acid affinity columns
US5959098A (en) * 1996-04-17 1999-09-28 Affymetrix, Inc. Substrate preparation process
AU5250098A (en) * 1996-11-08 1998-06-10 Ikonos Corporation Chemical functionalization of surfaces
US6887665B2 (en) * 1996-11-14 2005-05-03 Affymetrix, Inc. Methods of array synthesis
US6054504A (en) * 1997-12-31 2000-04-25 Hydromer, Inc. Biostatic coatings for the reduction and prevention of bacterial adhesion
US6287776B1 (en) * 1998-02-02 2001-09-11 Signature Bioscience, Inc. Method for detecting and classifying nucleic acid hybridization
US6262216B1 (en) * 1998-10-13 2001-07-17 Affymetrix, Inc. Functionalized silicon compounds and methods for their synthesis and use
US6472541B2 (en) * 1998-11-20 2002-10-29 The Regents Of The University Of California Protecting groups with increased photosensitivities
US6191046B1 (en) * 1999-03-11 2001-02-20 Advanced Micro Devices, Inc. Deposition of an oxide layer to facilitate photoresist rework on polygate layer
US6824866B1 (en) * 1999-04-08 2004-11-30 Affymetrix, Inc. Porous silica substrates for polymer synthesis and assays
US6358684B1 (en) * 1999-08-27 2002-03-19 Pe Corporation UV excitable fluorescent energy transfer dyes
EP1235932A2 (en) * 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US6833450B1 (en) * 2000-03-17 2004-12-21 Affymetrix, Inc. Phosphite ester oxidation in nucleic acid array preparation
US6806361B1 (en) * 2000-03-17 2004-10-19 Affymetrix, Inc. Methods of enhancing functional performance of nucleic acid arrays
US7005259B1 (en) * 2000-06-01 2006-02-28 Affymetrix, Inc. Methods for array preparation using substrate rotation
WO2002020150A2 (en) * 2000-09-11 2002-03-14 Affymetrix, Inc. Photocleavable protecting groups
US6841333B2 (en) * 2002-11-01 2005-01-11 3M Innovative Properties Company Ionic photoacid generators with segmented hydrocarbon-fluorocarbon sulfonate anions
US7385050B2 (en) * 2003-08-30 2008-06-10 Agilent Technologies, Inc. Cleavable linker for polynucleotide synthesis
US8338093B2 (en) * 2004-12-31 2012-12-25 Affymetrix, Inc. Primer array synthesis and validation
US7547775B2 (en) * 2004-12-31 2009-06-16 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
US7291471B2 (en) * 2005-11-21 2007-11-06 Agilent Technologies, Inc. Cleavable oligonucleotide arrays
US7951601B2 (en) * 2005-12-28 2011-05-31 Affymetrix, Inc. Oxide layers on silicon substrates for effective confocal laser microscopy
CA2629586C (en) * 2007-04-20 2016-05-24 F.Hoffmann-La Roche Ag Adsorption of nucleic acids to solid phases under low-salt conditions

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994010128A1 (en) * 1992-11-02 1994-05-11 Affymax Technologies N.V. Novel photoreactive protecting groups
US5763599A (en) * 1994-12-16 1998-06-09 Wolfgang Pfleiderer Nucleoside derivatives with photolabile protective groups

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
GRIEGRICH H ET AL: "New photolabile protecting groups in nucleoside and nucleotide chemistry - synthesis, cleavage mechanisms and applications" NUCLEOSIDES & NUCLEOTIDES, MARCEL DEKKER, INC, US, vol. 17, no. 9-11, 1998, pages 1987-1996, XP002159918 ISSN: 0732-8311 *

Cited By (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003035664A2 (en) * 2001-10-25 2003-05-01 Chemogenix Gmbh Method for covalently attaching nucleosides and/or nucleotides on surfaces and method for determining coupling yields in the synthesis of nucleotides
WO2003035664A3 (en) * 2001-10-25 2003-10-09 Chemogenix Gmbh Method for covalently attaching nucleosides and/or nucleotides on surfaces and method for determining coupling yields in the synthesis of nucleotides
WO2004058393A2 (de) * 2002-12-23 2004-07-15 Febit Biotech Gmbh Verfahren zum validierten aufbau von arrays
WO2004058391A2 (de) * 2002-12-23 2004-07-15 Febit Biotech Gmbh Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren
WO2004058392A2 (de) * 2002-12-23 2004-07-15 Febit Ag Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
WO2004058391A3 (de) * 2002-12-23 2004-08-26 Febit Ag Photoaktivierbare zweistufige schutzgruppen für die synthese von biopolymeren
WO2004058392A3 (de) * 2002-12-23 2004-08-26 Febit Ag Intramolekular triplettsensibilisierte o-nitrophenylethyl-photoschutzgruppen
US7737089B2 (en) 2002-12-23 2010-06-15 Febit Holding Gmbh Photoactivatable two-stage protective groups for the synthesis of biopolymers
WO2004058393A3 (de) * 2002-12-23 2004-09-10 Febit Ag Verfahren zum validierten aufbau von arrays
US8445734B2 (en) 2003-02-21 2013-05-21 Nigu Chemie Gmbh Photolabile protective groups for improved processes to prepare oligonucleotide arrays
GB2414237A (en) * 2003-02-21 2005-11-23 Nigu Chemie Gmbh Novel photolabile protective groups for improved processes to prepare oligonucleotide arrays
GB2414237B (en) * 2003-02-21 2007-04-11 Nigu Chemie Gmbh Novel photolabile protective groups for improved processes to prepare oligonucleotide arrays
WO2004074300A3 (en) * 2003-02-21 2004-12-29 Nigu Chemie Gmbh Novel photolabile protective groups for improved processes to prepare oligonucleotide arrays
WO2004074300A2 (en) * 2003-02-21 2004-09-02 Nigu Chemie Gmbh Novel photolabile protective groups for improved processes to prepare oligonucleotide arrays
US7759513B2 (en) 2003-02-21 2010-07-20 Nigu Chemie Gmbh Photolabile protective groups for improved processes to prepare oligonucleotide arrays
EP1833987A4 (en) * 2004-12-15 2009-03-11 Yeda Res & Dev A single-step platform for on-chip integration of bio-molecules
EP1833987A2 (en) * 2004-12-15 2007-09-19 Yeda Research and Development Co. Ltd. at The Weizmann Institute of Science A single-step platform for on-chip integration of bio-molecules
US8133987B2 (en) 2004-12-31 2012-03-13 Affymetrix, Inc. Primer array synthesis and validation
US8338093B2 (en) 2004-12-31 2012-12-25 Affymetrix, Inc. Primer array synthesis and validation
US8338585B2 (en) 2004-12-31 2012-12-25 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
US8101737B2 (en) 2004-12-31 2012-01-24 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
US8729251B2 (en) 2004-12-31 2014-05-20 Affymetrix, Inc. Parallel preparation of high fidelity probes in an array format
WO2013050795A1 (en) * 2011-10-03 2013-04-11 Femtonics Kft. Use of photoactive compounds
WO2014150845A1 (en) * 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Photocleavable deoxynucleotides with high-resolution control of deprotection kinetics
US10007182B2 (en) 2016-01-11 2018-06-26 Samsung Electronics Co., Ltd. Photoresist composition and method of manufacturing semiconductor device using the same

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