WO2002018655A3 - Compositions and methods for the generation of single stranded cdna microarrays with accurate universal quantitation reference - Google Patents

Compositions and methods for the generation of single stranded cdna microarrays with accurate universal quantitation reference Download PDF

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Publication number
WO2002018655A3
WO2002018655A3 PCT/US2001/027021 US0127021W WO0218655A3 WO 2002018655 A3 WO2002018655 A3 WO 2002018655A3 US 0127021 W US0127021 W US 0127021W WO 0218655 A3 WO0218655 A3 WO 0218655A3
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WO
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Patent type
Prior art keywords
single stranded
compositions
generation
methods
stranded cdna
Prior art date
Application number
PCT/US2001/027021
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French (fr)
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WO2002018655A2 (en )
Inventor
Wei Zhang
Limei Hu
Stanley R Hamilton
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Univ Texas
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00497Features relating to the solid phase supports
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277Apparatus
    • B01J2219/00497Features relating to the solid phase supports
    • B01J2219/00527Sheets
    • B01J2219/00529DNA chips
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00596Solid-phase processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00603Making arrays on substantially continuous surfaces
    • B01J2219/00659Two-dimensional arrays
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides
    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL CHEMISTRY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICO LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/04Libraries containing only organic compounds
    • C40B40/06Libraries containing nucleotides or polynucleotides, or derivatives thereof

Abstract

Single stranded nucleic acid oligonucleotides are immobilized on a solid support to provide a DNA chip, or microarray of single stranded DNA immobilized on a solid support, for hybridizing oligonucleotides. A universal and quantitatively reproducible hybridization reference oligonucleotide is used for data normalization and for quality control of the array printing process.
PCT/US2001/027021 2000-09-01 2001-08-30 Compositions and methods for the generation of single stranded cdna microarrays with accurate universal quantitation reference WO2002018655A3 (en)

Priority Applications (2)

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US65444500 true 2000-09-01 2000-09-01
US09/654,445 2000-09-01

Publications (2)

Publication Number Publication Date
WO2002018655A2 true WO2002018655A2 (en) 2002-03-07
WO2002018655A3 true true WO2002018655A3 (en) 2003-08-21

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PCT/US2001/027021 WO2002018655A3 (en) 2000-09-01 2001-08-30 Compositions and methods for the generation of single stranded cdna microarrays with accurate universal quantitation reference

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WO (1) WO2002018655A3 (en)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7138506B2 (en) 2001-05-09 2006-11-21 Genetic Id, Na, Inc. Universal microarray system
KR100794698B1 (en) 2001-06-28 2008-01-14 (주)바이오니아 Quality control method for biological chip
KR100830926B1 (en) * 2002-03-27 2008-05-22 유재천 Bio-Disc, Bio-Driver apparatus, and Assay method using the same
US20040185445A1 (en) 2003-03-19 2004-09-23 Ye Fang Universal readout for target identification using biological microarrays
US20040229226A1 (en) * 2003-05-16 2004-11-18 Reddy M. Parameswara Reducing microarray variation with internal reference spots
JPWO2005106029A1 (en) * 2004-04-30 2008-03-13 オリンパス株式会社 The method of analysis Nucleic Acids
US8173367B2 (en) 2004-10-18 2012-05-08 Sherri Boucher In situ dilution of external controls for use in microarrays
CN101297199B (en) * 2005-10-28 2011-12-28 三菱丽阳株式会社 Probe mounting location confirmation method of nucleic acid arrays
FR2894596B1 (en) * 2005-12-13 2012-08-10 Centre Nat Rech Scient Method for self-calibration of biochips
US9169515B2 (en) 2010-02-19 2015-10-27 Life Technologies Corporation Methods and systems for nucleic acid sequencing validation, calibration and normalization
GB201102385D0 (en) 2011-02-10 2011-03-30 Biocule Scotland Ltd Two-dimensional gel electrophoresis apparatus and method
CN103103623B (en) * 2013-03-01 2014-03-19 山东维真生物科技有限公司 Adenovirus chip

Citations (7)

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Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6013449A (en) * 1997-11-26 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Probe-based analysis of heterozygous mutations using two-color labelling
WO2001042512A2 (en) * 1999-11-24 2001-06-14 Incyte Genomics, Inc. Normalization controls and duplex probes for quantitative hybridization reactions
WO2001071039A2 (en) * 2000-03-20 2001-09-27 Incyte Genomics, Inc. Combined polynucleotide sequences as discrete assay endpoints
EP1180548A2 (en) * 2000-07-31 2002-02-20 Agilent Technologies, Inc. Array based methods for synthesizing nucleic acid mixtures

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993017126A1 (en) * 1992-02-19 1993-09-02 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5795714A (en) * 1992-11-06 1998-08-18 Trustees Of Boston University Method for replicating an array of nucleic acid probes
US5919626A (en) * 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6013449A (en) * 1997-11-26 2000-01-11 The United States Of America As Represented By The Department Of Health And Human Services Probe-based analysis of heterozygous mutations using two-color labelling
WO2001042512A2 (en) * 1999-11-24 2001-06-14 Incyte Genomics, Inc. Normalization controls and duplex probes for quantitative hybridization reactions
WO2001071039A2 (en) * 2000-03-20 2001-09-27 Incyte Genomics, Inc. Combined polynucleotide sequences as discrete assay endpoints
EP1180548A2 (en) * 2000-07-31 2002-02-20 Agilent Technologies, Inc. Array based methods for synthesizing nucleic acid mixtures

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BULYK M L ET AL: "Quantifying DNA-protein interactions by double-stranded DNA arrays", NATURE BIOTECHNOLOGY, NATURE PUBLISHING, US, vol. 17, June 1999 (1999-06-01), pages 573 - 577, XP002168458, ISSN: 1087-0156 *
DUGGAN D J ET AL: "EXPRESSION PROFILING USING CDNA MICROARRAYS", NATURE GENETICS, NEW YORK, NY, US, vol. 21, no. SUPPL, January 1999 (1999-01-01), pages 10 - 14, XP000865980, ISSN: 1061-4036 *

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