WO2002010751A3 - Transfer microdissection - Google Patents

Transfer microdissection Download PDF

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Publication number
WO2002010751A3
WO2002010751A3 PCT/US2001/008095 US0108095W WO0210751A3 WO 2002010751 A3 WO2002010751 A3 WO 2002010751A3 US 0108095 W US0108095 W US 0108095W WO 0210751 A3 WO0210751 A3 WO 0210751A3
Authority
WO
WIPO (PCT)
Prior art keywords
biomolecules
transfer layer
transfer
interest
altered
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/008095
Other languages
French (fr)
Other versions
WO2002010751A2 (en
WO2002010751A8 (en
Inventor
Michael R Emmert-Buck
Chad R Haldeman-Englert
Robert F Bonner
Lance A Liotta
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GOVERNMENT OF United States, ASREPRESENTED BY SECRETARY DEPARTMENT OF HEALTH
GOVERNMENT OF US SEC DEP O
Original Assignee
GOVERNMENT OF United States, ASREPRESENTED BY SECRETARY DEPARTMENT OF HEALTH
GOVERNMENT OF US SEC DEP O
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GOVERNMENT OF United States, ASREPRESENTED BY SECRETARY DEPARTMENT OF HEALTH, GOVERNMENT OF US SEC DEP O filed Critical GOVERNMENT OF United States, ASREPRESENTED BY SECRETARY DEPARTMENT OF HEALTH
Priority to US10/333,374 priority Critical patent/US20040053326A1/en
Priority to AU2001245700A priority patent/AU2001245700A1/en
Priority to CA002415864A priority patent/CA2415864A1/en
Priority to EP01918647A priority patent/EP1305622A2/en
Publication of WO2002010751A2 publication Critical patent/WO2002010751A2/en
Publication of WO2002010751A3 publication Critical patent/WO2002010751A3/en
Anticipated expiration legal-status Critical
Publication of WO2002010751A8 publication Critical patent/WO2002010751A8/en
Ceased legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2813—Producing thin layers of samples on a substrate, e.g. smearing, spinning-on
    • G01N2001/2833—Collecting samples on a sticky, tacky, adhesive surface
    • G01N2001/284—Collecting samples on a sticky, tacky, adhesive surface using local activation of adhesive, i.e. Laser Capture Microdissection

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present disclosure concerns methods, systems, and devices for analyzing a biological material, such as a cellular or other specimen. In one disclosed example, the method selectively transfers biomolecules from a target region of interest in a biological sample (such as a tissue section). The transfer may occur, for example, by selectively focally altering a characteristic of a transfer layer adjacent the target region, such that the biomolecules can move through the altered area of the transfer layer. In particular examples, the transfer layer is altered by focally increasing a permeability of the transfer layer, for example by removing a focal portion of the transfer layer, and transporting the biomolecules through the altered region of the transfer layer, to microdissect the biomolecules of interest from the biological sample. In yet other embodiments, the microdissected biomolecules can be applied to an analysis substrate containing an identification molecule, such as a nucleic acid array, layered expression scan, or wells containing antibodies. Transfer microdissection allows biomolecules from regions of interest in the biological specimen to be selectively analyzed. For example, nests of highly atypical or metastatic cells in a tumor section can be analyzed for differential expression of certain proteins.
PCT/US2001/008095 2000-07-26 2001-03-14 Transfer microdissection Ceased WO2002010751A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/333,374 US20040053326A1 (en) 2001-03-14 2001-03-14 Transfer microdessection
AU2001245700A AU2001245700A1 (en) 2000-07-26 2001-03-14 Transfer microdissection
CA002415864A CA2415864A1 (en) 2000-07-26 2001-03-14 Transfer microdissection
EP01918647A EP1305622A2 (en) 2000-07-26 2001-03-14 Transfer microdissection

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22090400P 2000-07-26 2000-07-26
US60/220,904 2000-07-26

Publications (3)

Publication Number Publication Date
WO2002010751A2 WO2002010751A2 (en) 2002-02-07
WO2002010751A3 true WO2002010751A3 (en) 2002-04-25
WO2002010751A8 WO2002010751A8 (en) 2004-03-04

Family

ID=22825494

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/008095 Ceased WO2002010751A2 (en) 2000-07-26 2001-03-14 Transfer microdissection

Country Status (4)

Country Link
EP (1) EP1305622A2 (en)
AU (1) AU2001245700A1 (en)
CA (1) CA2415864A1 (en)
WO (1) WO2002010751A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1521961A2 (en) * 2002-07-05 2005-04-13 The University of Chicago Characterization of biochips containing self-assembled monolayers with maldi-tof-ms
US20040077073A1 (en) * 2002-10-18 2004-04-22 Melvin Schindler Methods and apparatus for interactive micromanipulation of biological materials
US7709047B2 (en) 2003-01-24 2010-05-04 The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
US7695752B2 (en) 2003-01-24 2010-04-13 The Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services Target activated microtransfer
DE10358565B4 (en) * 2003-12-15 2007-06-28 P.A.L.M. Microlaser Technologies Ag A receiving element for receiving an object released from a biological mass by means of laser radiation and method for obtaining and processing a biological object
DE102004041941B4 (en) * 2004-08-30 2007-01-11 P.A.L.M. Microlaser Technologies Ag Method for obtaining biological objects with a recording unit
FR2883070B1 (en) * 2005-03-08 2007-05-18 Inst Nat Sante Rech Med METHOD AND SYSTEM FOR IN VITRO DETERMINATION OF THE REACTIVITY OF AN ENTITY OF INTEREST ON A THREE-DIMENSIONAL FUNCTIONAL ELEMENT
DE102005061561A1 (en) 2005-12-22 2007-06-28 P.A.L.M. Microlaser Technologies Ag Microdessection method for separation of e.g. individual cells, of e.g. biological preparation, involves cutting preparation through laser irradiation to divide cut layer into low cut and non cut sections, where non cut section is removed
DE102006000934A1 (en) * 2006-01-05 2007-07-12 P.A.L.M. Microlaser Technologies Ag Microdissection and microdissection system
DE102013212811A1 (en) 2013-07-01 2015-01-08 Leica Microsystems Cms Gmbh Laser microdissection system and assay procedure for nucleic acid containing samples
ES3038460T3 (en) * 2017-04-07 2025-10-13 Avoneaux Medical Inst Llc High precision micropurification system and methods of use

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013838A1 (en) * 1995-10-10 1997-04-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Isolation of cellular material under microscopic visualization
WO2001007915A2 (en) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Layered device with capture regions for cellular analysis

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997013838A1 (en) * 1995-10-10 1997-04-17 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Isolation of cellular material under microscopic visualization
WO2001007915A2 (en) * 1999-07-26 2001-02-01 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Layered device with capture regions for cellular analysis

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BANKS R E ET AL: "THE POTENTIAL USE OF LASER CAPTURE MICRODISSECTION TO SELECTIVELY OBTAIN DISTINCT POPULATIONS OF CELLS FOR PROTEOMIC ANALYSIS - PRELIMINARY FINDINGS", ELECTROPHORESIS, WEINHEIM, DE, vol. 20, no. 4/5, April 1999 (1999-04-01), pages 689 - 700, XP000925546, ISSN: 0173-0835 *
EMMERT-BUCK M R ET AL: "LASER CAPTURE MICRODISSECTION", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE,, US, vol. 274, no. 5289, 8 November 1996 (1996-11-08), pages 998 - 1001, XP000644727, ISSN: 0036-8075 *
ENGLERT CHAD R ET AL: "Layered expression scanning: Rapid molecular profiling of tumor samples", CANCER RESEARCH, AMERICAN ASSOCIATION FOR CANCER RESEARCH, BALTIMORE, MD, US, vol. 60, no. 6, 15 March 2000 (2000-03-15), pages 1526 - 1530, XP002156603, ISSN: 0008-5472 *

Also Published As

Publication number Publication date
WO2002010751A2 (en) 2002-02-07
EP1305622A2 (en) 2003-05-02
AU2001245700A1 (en) 2002-02-13
WO2002010751A8 (en) 2004-03-04
CA2415864A1 (en) 2002-02-07

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