WO2001096382A3 - Thermostable cellulase - Google Patents

Thermostable cellulase Download PDF

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Publication number
WO2001096382A3
WO2001096382A3 PCT/IS2001/000012 IS0100012W WO0196382A3 WO 2001096382 A3 WO2001096382 A3 WO 2001096382A3 IS 0100012 W IS0100012 W IS 0100012W WO 0196382 A3 WO0196382 A3 WO 0196382A3
Authority
WO
WIPO (PCT)
Prior art keywords
variant
variants
amino acid
thermostable
thermostable cellulase
Prior art date
Application number
PCT/IS2001/000012
Other languages
French (fr)
Other versions
WO2001096382A2 (en
Inventor
Kryzysztof B Wicher
Olof Peder Holst
Maher Youssef Abou Hachem
Eva Margareta Nordber Karlsson
Gudmundur O Hreggvidsson
Original Assignee
Prokaria Ehf
Kryzysztof B Wicher
Olof Peder Holst
Maher Youssef Abou Hachem
Eva Margareta Nordber Karlsson
Gudmundur O Hreggvidsson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Prokaria Ehf, Kryzysztof B Wicher, Olof Peder Holst, Maher Youssef Abou Hachem, Eva Margareta Nordber Karlsson, Gudmundur O Hreggvidsson filed Critical Prokaria Ehf
Priority to EP01938538A priority Critical patent/EP1297004A2/en
Priority to AU2001264212A priority patent/AU2001264212A1/en
Priority to US10/003,759 priority patent/US6812018B2/en
Publication of WO2001096382A2 publication Critical patent/WO2001096382A2/en
Publication of WO2001096382A3 publication Critical patent/WO2001096382A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Abstract

Disclosed are variant thermostable cellulases, nucleic acids encoding the variants and methods for producing the variants. The variant thermostable cellulases have the amino acid sequence of a glycosyl hydrolase of family 12 wherein one or more amino acid residues which are not part of the catalytic domain are deleted. In specific embodiments, the variant thermostable cellulase is a variant of Cell2A of Rhodothermus marinus.
PCT/IS2001/000012 2000-06-15 2001-06-15 Thermostable cellulase WO2001096382A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01938538A EP1297004A2 (en) 2000-06-15 2001-06-15 Thermostable cellulase
AU2001264212A AU2001264212A1 (en) 2000-06-15 2001-06-15 Thermostable cellulase
US10/003,759 US6812018B2 (en) 2000-06-15 2001-10-23 Thermostable cellulase

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US59488400A 2000-06-15 2000-06-15
US09/594,884 2000-06-15

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
US59488400A Continuation-In-Part 2000-06-15 2000-06-15

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/003,759 Continuation-In-Part US6812018B2 (en) 2000-06-15 2001-10-23 Thermostable cellulase

Publications (2)

Publication Number Publication Date
WO2001096382A2 WO2001096382A2 (en) 2001-12-20
WO2001096382A3 true WO2001096382A3 (en) 2002-04-18

Family

ID=24380817

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/IS2001/000012 WO2001096382A2 (en) 2000-06-15 2001-06-15 Thermostable cellulase

Country Status (4)

Country Link
US (1) US6812018B2 (en)
EP (1) EP1297004A2 (en)
AU (1) AU2001264212A1 (en)
WO (1) WO2001096382A2 (en)

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US20030199072A1 (en) * 2002-04-19 2003-10-23 Prokaria, Itd. Crystal and structure of a thermostable glycosol hydrolase and use thereof, and modified proteins
US20050054752A1 (en) * 2003-09-08 2005-03-10 O'brien John P. Peptide-based diblock and triblock dispersants and diblock polymers
WO2005100557A1 (en) * 2004-04-16 2005-10-27 Ab Enzymes Oy Method and dna constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
US7348172B2 (en) 2004-04-16 2008-03-25 Ab Enzymes Oy Method and DNA constructs for increasing the production level of carbohydrate degrading enzymes in filamentous fungi
ES2554467T3 (en) * 2004-12-30 2015-12-21 Danisco Us Inc. Variants of CBH2 cellulases from Hypocrea jecorina
US8093030B2 (en) * 2005-10-06 2012-01-10 Lucigen Corporation Thermostable viral polymerases and methods of use
US7803601B2 (en) * 2006-10-04 2010-09-28 Board Of Regents, The University Of Texas Production and secretion of glucose in photosynthetic prokaryotes (cyanobacteria)
US20080113413A1 (en) * 2006-10-04 2008-05-15 Board Of Regents, The University Of Texas System Expression of Foreign Cellulose Synthase Genes in Photosynthetic Prokaryotes (Cyanobacteria)
US20080124767A1 (en) * 2006-10-04 2008-05-29 Board Of Regents, The University Of Texas At Austin Production and Secretion of Sucrose in Photosynthetic Prokaryotes (Cyanobacteria)
US20080085536A1 (en) * 2006-10-04 2008-04-10 Board Of Regents, The University Of Texas System Production of Cellulose in Halophilic Photosynthetic Prokaryotes (Cyanobacteria)
CN101568551B (en) * 2006-11-06 2016-05-18 科学与工业研究委员会 Meso-active thermo-stable protein and design thereof and biological synthesis method in restructuring
US8583380B2 (en) 2008-09-05 2013-11-12 Aueon, Inc. Methods for stratifying and annotating cancer drug treatment options
US9309556B2 (en) 2010-09-24 2016-04-12 The Board Of Trustees Of The Leland Stanford Junior University Direct capture, amplification and sequencing of target DNA using immobilized primers
BR112014013236A2 (en) 2011-12-02 2017-06-13 Bp Corp North America Inc compositions and methods for biomass liquefaction
CN103740662B (en) * 2012-05-10 2015-08-19 江南大学 A kind of method improving enzyme heat stability
CN103805579B (en) * 2014-02-19 2016-05-18 江南大学 A kind of heat-staple amylase mutant and its preparation method and application
JP6268486B2 (en) 2014-08-04 2018-01-31 本田技研工業株式会社 Super thermostable endoglucanase belonging to GH family 12
JP6315807B2 (en) 2014-08-04 2018-04-25 本田技研工業株式会社 Super thermostable endoglucanase belonging to GH family 12
JP6552098B2 (en) * 2015-08-20 2019-07-31 本田技研工業株式会社 Super thermostable endoglucanase
JP6244597B2 (en) 2015-08-21 2017-12-13 本田技研工業株式会社 Super thermostable endoglucanase

Citations (5)

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WO1997044361A1 (en) * 1996-05-22 1997-11-27 Recombinant Biocatalysis, Inc. Endoglucanases
WO1998033895A1 (en) * 1997-01-31 1998-08-06 Novo Nordisk A/S A thermostable endo-beta-1,4-glucanase
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WO2000009707A1 (en) * 1998-06-24 2000-02-24 Genencor International, Inc. Cellulase producing actinomycetes, cellulase produced therefrom and method of producing same
WO2000037614A2 (en) * 1998-12-18 2000-06-29 Genencor International, Inc. Variants of egiii-like cellulase and compositions thereof

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WO1998033895A1 (en) * 1997-01-31 1998-08-06 Novo Nordisk A/S A thermostable endo-beta-1,4-glucanase
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WO2000037614A2 (en) * 1998-12-18 2000-06-29 Genencor International, Inc. Variants of egiii-like cellulase and compositions thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
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Also Published As

Publication number Publication date
US6812018B2 (en) 2004-11-02
WO2001096382A2 (en) 2001-12-20
EP1297004A2 (en) 2003-04-02
AU2001264212A1 (en) 2001-12-24
US20020102699A1 (en) 2002-08-01

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