WO2001091817A3 - Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien - Google Patents

Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien Download PDF

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Publication number
WO2001091817A3
WO2001091817A3 PCT/DE2001/002109 DE0102109W WO0191817A3 WO 2001091817 A3 WO2001091817 A3 WO 2001091817A3 DE 0102109 W DE0102109 W DE 0102109W WO 0191817 A3 WO0191817 A3 WO 0191817A3
Authority
WO
WIPO (PCT)
Prior art keywords
confluence
determination
cell layer
hybrid construct
porous biomaterials
Prior art date
Application number
PCT/DE2001/002109
Other languages
English (en)
French (fr)
Other versions
WO2001091817A2 (de
Inventor
Manrico Paulitschke
Axel Rademacher
Original Assignee
Cell Lining Ges Fuer Zellkulti
Manrico Paulitschke
Axel Rademacher
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cell Lining Ges Fuer Zellkulti, Manrico Paulitschke, Axel Rademacher filed Critical Cell Lining Ges Fuer Zellkulti
Priority to EP01955196A priority Critical patent/EP1290134B1/de
Priority to DE50111590T priority patent/DE50111590D1/de
Publication of WO2001091817A2 publication Critical patent/WO2001091817A2/de
Publication of WO2001091817A3 publication Critical patent/WO2001091817A3/de
Priority to US10/307,773 priority patent/US6946290B2/en

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
    • A61F2/02—Prostheses implantable into the body
    • A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
    • A61F2/06—Blood vessels
    • A61F2/062—Apparatus for the production of blood vessels made from natural tissue or with layers of living cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Abstract

Die Erfindung betrifft ein neuartiges Verfahren, welches die semiquantitative Bestimmung der Konfluenz einer Zellschicht auf der gesamten Oberfläche eines porösen Biomaterials ermöglicht, ohne dabei Teile oder gar das gesamte Hybridkonstrukt irreversibel zu schädigen Damit wird erstmalig ein Verfahren beschrieben, das die Bildung der Konfluenz und die Konfluenz selbst ohne Zerstörung des Hybridkonstruktes nachweisen kann und somit erlaubt, das fertige Hybridkonstrukt erst nach Feststellung des Erreichens der Konfluenz den essentiellen Wachstumsbedingungen zu entziehen und sofort seiner weiteren Verwendung zuzuführen.
PCT/DE2001/002109 2000-06-02 2001-06-01 Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien WO2001091817A2 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01955196A EP1290134B1 (de) 2000-06-02 2001-06-01 Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien
DE50111590T DE50111590D1 (de) 2000-06-02 2001-06-01 Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien
US10/307,773 US6946290B2 (en) 2000-06-02 2002-12-02 Method for the determination of the confluence of a cell layer on porous biomaterials

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10028118.4 2000-06-02
DE10028118A DE10028118C2 (de) 2000-06-02 2000-06-02 Verfahren zur Bestimmung der Konfluenz einer Zellschicht auf porösen Biomaterialien

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/307,773 Continuation US6946290B2 (en) 2000-06-02 2002-12-02 Method for the determination of the confluence of a cell layer on porous biomaterials

Publications (2)

Publication Number Publication Date
WO2001091817A2 WO2001091817A2 (de) 2001-12-06
WO2001091817A3 true WO2001091817A3 (de) 2002-11-28

Family

ID=7644949

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/002109 WO2001091817A2 (de) 2000-06-02 2001-06-01 Verfahren zur bestimmung der konfluenz einer zellschicht auf porösen biomaterialien

Country Status (5)

Country Link
US (1) US6946290B2 (de)
EP (1) EP1290134B1 (de)
AT (1) ATE347583T1 (de)
DE (2) DE10028118C2 (de)
WO (1) WO2001091817A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6946292B2 (en) * 2000-10-06 2005-09-20 Kyowa Hakko Kogyo Co., Ltd. Cells producing antibody compositions with increased antibody dependent cytotoxic activity
DE102004054125B4 (de) * 2004-11-08 2011-01-05 Minucells And Minutissue Vertriebs Gmbh Gradientenkammer zum Kultivieren und/oder Differenzieren von Zellen/Geweben

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911713A (en) * 1986-03-26 1990-03-27 Sauvage Lester R Method of making vascular prosthesis by perfusion
FR2665175A1 (fr) * 1990-07-27 1992-01-31 Rosdy Martin Equivalent d'epiderme, son procede d'obtention et son utilisation.
WO1993001843A1 (en) * 1991-07-25 1993-02-04 University Of Leicester Preparing grafts for implantation
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4911713A (en) * 1986-03-26 1990-03-27 Sauvage Lester R Method of making vascular prosthesis by perfusion
FR2665175A1 (fr) * 1990-07-27 1992-01-31 Rosdy Martin Equivalent d'epiderme, son procede d'obtention et son utilisation.
WO1993001843A1 (en) * 1991-07-25 1993-02-04 University Of Leicester Preparing grafts for implantation
WO1997049799A1 (en) * 1996-06-27 1997-12-31 Advanced Tissue Sciences, Inc. Apparatus and method for sterilizing, seeding, culturing, storing, shipping and testing tissue, synthetic or native, vascular grafts

Also Published As

Publication number Publication date
DE10028118A1 (de) 2001-12-13
EP1290134B1 (de) 2006-12-06
US20030077574A1 (en) 2003-04-24
EP1290134A2 (de) 2003-03-12
DE10028118C2 (de) 2003-10-16
WO2001091817A2 (de) 2001-12-06
DE50111590D1 (de) 2007-01-18
ATE347583T1 (de) 2006-12-15
US6946290B2 (en) 2005-09-20

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