WO2001089699A1 - Improvements to microcontainers for clinical assays - Google Patents

Improvements to microcontainers for clinical assays Download PDF

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Publication number
WO2001089699A1
WO2001089699A1 PCT/ES2000/000339 ES0000339W WO0189699A1 WO 2001089699 A1 WO2001089699 A1 WO 2001089699A1 ES 0000339 W ES0000339 W ES 0000339W WO 0189699 A1 WO0189699 A1 WO 0189699A1
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WO
Grant status
Application
Patent type
Prior art keywords
body
microcontainer
microcontainers
improvements
seat
Prior art date
Application number
PCT/ES2000/000339
Other languages
Spanish (es)
French (fr)
Inventor
Lucas Victor Grifols
Original Assignee
Lucas Victor Grifols
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

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Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • B01L3/50825Closing or opening means, corks, bungs

Abstract

The invention relates to improvements to microcontainers. According to said improvements, the axial retention means of the stop body are disposed in the seat thereof, consisting of the mechanical interference between said retention body and the seat area in the intermediate narrowing of the microcontainer, which extend to limited areas on the periphery of the retention body with the purpose of freeing gaps intended for the passage of the sample by centrifugation from the mouth in the inlet of the microcontainer to the cylindrical or tubular body containing the reactant.

Description

IMPROVEMENTS IN ANALYTICAL Clini microcontainers £ Q_g

DESCRIPTIVE MEMORY

The present invention is intended to disclose some improvements in clinical analysis microcontainers intended especially blood group contributing notable characteristics of novelty and inventive step to the function for which they are intended activity.

In particular, the improvements object of the present invention are intended for microcontainers for clinical analyzes in which a lower body of the carrier container of a reagent is provided, optionally with a built-in retention and filtration gel being intended to subsequently receive the sample analyzing in the upper mouth of the container, the sample must reach the reagent contained in the lower body after suitable centrifugation. In a preferred version, the microcontainers form a set as "plate" or "card" which facilitates handling.

Previously they have been released in the market microcontainers for the conduct of clinical analysis. For example, European Patent 194,212 disclosed microcontainers of substantially cylindrical shape in its upper part inferiorly narrow, after an intermediate conical region. European Patent 305,337 also disclosed a similar microcontainers endowed with an optionally flattened side, adapted on a flat plate. All microcontainers indicated are open at the top at the time of being used for its specific function, based both documents that in the case of a blood test, RBCs positioned above the mixture of particles or gel embedded in the agglutinating serum penetrate by centrifugation into the mentioned layer formed by the reagent and particles and, as they descend, they are agglutinating if the reactive serum and the erythrocytes are of the same specificity and, by virtue of this binding, is produce large lumps that can not penetrate into the granular mass and, therefore, are visible above the layer of granules or gel. When there is no reaction, the red cells, which are not agglutinated, they have a smaller size and lumps passing through the gel layer and particles to the bottom of the tube.

However, such known microcontainers can present certain drawbacks by ineffective retention, with little safe location of the reagent in the cylindrical lower portion of the microcontainer, so that in practical use cards microcontainers of this type are presented frequently use situations where for handling errors, drop card or other, the reagent may be partially or completely expelled from the cylindrical body of the microcontainer, so that it is not in suitable condition to perform its function to receiving the sample to be analyzed may produce erroneous results, with the serious consequences which this can entail.

Another drawback that occurs in some of the microcontainers for analysis currently known is the possibility that the introduction of the sample to be analyzed take place with excessive speed or with incorrect positioning of the pipette does not ensure smooth and regular back entrance the test sample by centrifugation, producing extemporaneous mixtures can also result in erroneous readings.

It is seen, therefore, that the currently known microcontainers is possible to have erroneous results of the analysis can be significantly serious in its consequences. In particular, in the case of blood tests, an error in the results of these can lead to fatal consequences for the patient as observed by clinical experience of recent years.

To solve the aforementioned disadvantages, the present applicant itself presented at the time the application for Spanish Utility Model n Q 9900372 which provided an element for controlled closure arranged between the top of the microcontainer or receiving cavity and the bottom thereof, so that communication of said upper and lower parts should necessarily take place by the action of centrifugation, given that the closure element interposed between the two chambers left free steps of reduced section which were sufficient for passage by centrifugation but which prevented free passage from the upper to the lower chamber of the microcontainer as occurred in practice camera. That invention already signified an important advance in providing for the provision of a body of intermediate stop between the upper mouth of the microcontainer and the lower body containing the reagent, in order to preclude the sudden entry of the sample to be analyzed within the body part It is containing the reagent.

The present invention is an improvement of the application of the Spanish Utility Model n Q 9900372 the present applicant, as in studies and research carried out by the inventor, although the invention consists of arranging a closing body controlled between upper mouth of the microcontainer and the lower body of it had clear positive effects, there was still the possibility that in the event that a bad maneuver of an unskilled operator is reached introduce the carrier pipette the sample to be analyzed too strength and / or too deep, this action could report the partial lifting of the intermediate closure body, allowing the accidental passage of the pipette towards the lower portion of the microcontainer, so that the reaction can not see, and may result in a reading fully erroneous, especially dangerous in the case of blood tests.

Therefore, the objective of the present invention is to achieve microcontainers for clinical analyzes which have not only sufficient protection features a sharp entry or against incorrect positioning of the test sample, but also avoided while loss of efficacy body intermediate stop, preventing possible detachment.

To achieve its objectives, the present invention discloses microcontainers for clinical analyzes in which the intermediate for controlled separation between the inlet mouth of the microcontainer and the lower body thereof, containing the reagent, the body is retained by a specific structure the receiving seat of said intermediate body, enabling step by centrifugation of the test sample from the mouth of introduction, through the narrow gaps controlled by the intermediate body, towards the lower body containing the reagent, prevents body outlet through its seat, ensuring, thus, the effectiveness desired.

Retaining said intermediate body preferably can be accomplished by the provision of longitudinal ribs in the seat area determining areas body support limitation that prevent their accidental removal and simultaneously determine interstices to permit passage of the sample into the lower body of the microcontainer in which the reagent is.

To summarize, the present improvements are characterized by the provision of means for axially retaining the body onto its seat constituted by the mechanical interference between said retaining body and the seat region therein in the intermediate narrowing of the microcontainer, which limited extend from the periphery of the retaining body to leave gaps free for the passage of the sample by centrifugation from the inlet opening towards the carrier body microcontainer reagent areas. Such interference can be achieved, for example, by the formation of accommodation in the front edges of longitudinal ribs arranged in the narrowing of the microcontainer, whose properties have conjugated shape of the side surface of the retaining body, permitting the introduction thereof by elasticity microcontainer and / or of the retaining body, or, as another example, by the formation of a prismatic housing in the narrowing microcontainer for receiving interference with a body of spherical or like retention.

For a better understanding attached, exemplary non-limiting explanatory, some drawings of a preferred embodiment of the present invention.

Figures 1 and 2 are longitudinal sections of a microcontainer which includes the present improvements as mutually perpendicular planes.

3 shows a plan view of the microcontainer itself.

Figures 4 and 5 are respective views similar to Figures 1 and 3 of an alternative embodiment of the present invention sections.

6 shows a plan view of the microcontainer represented in Figures 4 and 5.

As can be seen in the drawings, the present improvements are applicable to microcontainers in which it has a mouth or upper body 1 and lower casing 2 for containing the reagent, both being separated by a zone more -3- close in which it is located an intermediate body 4 which serves as a stop to prevent the entry of the sample can take place with excessive speed or with an error of placement of the dispensing pipette or nozzle, causing a possible mixture directly with the reagent container 2. The present improvements provide for the provision of means for the axial retention of the body 4 in its housing. This is achieved by different arrangements utilizing the elasticity of the microcontainer and of the body 4 to permit insertion of said retaining body -4-, normally a small sphere, in the service position thereof, overcoming the small interfering with the microcontainer by the resilience of the material thereof, permitting the introduction of said ball and the resilience of the material itself combined with the specific shape of the seat intended -4- retaining body which prevents exit of said retaining body. In the case represented in the mouth -3- several longitudinal ribs or partitions as represented by reference numerals -5-, -6-, -7- and -8-, in variable number are formed, which characterized at its front edge can be straight or may have respective recesses -20- matingly retaining body -4-, ie arcuately for the case that the retaining body is a spherical ball, as It is shown in the drawings.

In an alternative embodiment of the present invention shown in Figures 4 to 6, the mouth 9 of the microcontainer present a narrowing -10- whose cross shape may be prismatic, for example, square prismatic sides -11- -12 -, 13 and 14, being intended to receive the retaining body preferably shaped ball 15 which may be retained under pressure within said prismatic housing, the edges determining respective steps -16-, -17 -, 18 and 19 for communication between the mouth of the upper and the lower body of the microcontainer.

Retention stop body inside the prismatic housing can be made by simple elasticity, the body retention and the same vessel, or by conducting areas matingly designed to receive said retaining body after introduction. This form of retention is also applicable, as is obvious, the structure shown in Figures 1 to 3.

Claims

What is claimed
1. Improvements to microcontainers for clinical analyzes, of the type comprising a mouthpiece and a lower container top generally cylindrical carrier reagent with an intermediate stop body which determines controlled interstices for passage of the test sample by centrifugation into the carrier body reagent, characterized by the provision of means for axially retaining the body onto its seat constituted by the mechanical interference between said retaining body and the seat region therein in the intermediate narrowing microcontainer, extending zones limited the periphery of the retaining body to leave gaps free for the passage of the sample by centrifugation from the inlet opening towards the carrier body microcontainer reagent, preventing the accidental lifting of the intermediate stop body from its seat.
2. Improvements to microcontainers for clinical analysis according to claim 1, wherein the mechanical interference between the stop body and its seat in the microcontainer is achieved by the provision of accommodation in the front edges of longitudinal ribs arranged in the narrowing microcontainer, whose properties have conjugated shape of the side surface of the retaining body, permitting the introduction thereof by elasticity of the microcontainer and / or of the retaining body.
3. Improvements to microcontainers for clinical analysis according to claim 2, wherein the longitudinal partition walls have arcuate edges at their front housings for receiving a retaining body into a ball.
4. Improvements to microcontainers for clinical analysis according to claim 1, characterized by the formation of a prismatic housing in the narrowing microcontainer for receiving interference with a body of spherical or like retention.
PCT/ES2000/000339 2000-05-26 2000-09-11 Improvements to microcontainers for clinical assays WO2001089699A1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
ESP200001336 2000-05-26
ES200001336A ES2165322B1 (en) 2000-05-26 2000-05-26 Improvements in microcontainers for clinical analysis.

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001585930A JP3474558B6 (en) 2000-09-11 Improved micro-container for clinical analysis
EP20000956535 EP1206968A1 (en) 2000-05-26 2000-09-11 Improvements to microcontainers for clinical assays

Publications (1)

Publication Number Publication Date
WO2001089699A1 true true WO2001089699A1 (en) 2001-11-29

Family

ID=8493671

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/ES2000/000339 WO2001089699A1 (en) 2000-05-26 2000-09-11 Improvements to microcontainers for clinical assays

Country Status (3)

Country Link
EP (1) EP1206968A1 (en)
ES (1) ES2165322B1 (en)
WO (1) WO2001089699A1 (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US4417981A (en) * 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
EP0875202A2 (en) * 1997-04-30 1998-11-04 Becton Dickinson and Company Evacuated sample collection tube with aqueous additive
ES1042236U (en) * 1999-02-12 1999-08-16 Lucas Victor Grifols Device for carrying out agglutination tests.

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3935113A (en) * 1974-02-27 1976-01-27 Becton, Dickinson And Company Serum/plasma separator with centrifugal valve
US4417981A (en) * 1981-05-04 1983-11-29 Becton, Dickinson And Company Blood phase separator device
EP0875202A2 (en) * 1997-04-30 1998-11-04 Becton Dickinson and Company Evacuated sample collection tube with aqueous additive
ES1042236U (en) * 1999-02-12 1999-08-16 Lucas Victor Grifols Device for carrying out agglutination tests.

Also Published As

Publication number Publication date Type
EP1206968A1 (en) 2002-05-22 application
ES2165322B1 (en) 2003-02-16 grant
JP3474558B2 (en) 2003-12-08 grant
ES2165322A1 (en) 2002-03-01 application
JP2004506875A (en) 2004-03-04 application

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