WO2001089219A1 - Dispositif d'imagerie optique compact - Google Patents

Dispositif d'imagerie optique compact Download PDF

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Publication number
WO2001089219A1
WO2001089219A1 PCT/US2001/010823 US0110823W WO0189219A1 WO 2001089219 A1 WO2001089219 A1 WO 2001089219A1 US 0110823 W US0110823 W US 0110823W WO 0189219 A1 WO0189219 A1 WO 0189219A1
Authority
WO
WIPO (PCT)
Prior art keywords
carrying case
detection device
photon detection
dimension
configuration
Prior art date
Application number
PCT/US2001/010823
Other languages
English (en)
Inventor
Hashem Akhavan-Tafti
Jack Chambers
Richard S. Handley
Barry A. Schoenfelner
Original Assignee
Lumigen, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lumigen, Inc. filed Critical Lumigen, Inc.
Priority to US10/276,717 priority Critical patent/US20040051808A1/en
Priority to AU2001261012A priority patent/AU2001261012A1/en
Publication of WO2001089219A1 publication Critical patent/WO2001089219A1/fr

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Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B27/00—Optical systems or apparatus not provided for by any of the groups G02B1/00 - G02B26/00, G02B30/00
    • G02B27/02—Viewing or reading apparatus
    • G02B27/022—Viewing apparatus

Definitions

  • the present invention relates to a compact apparatus for digital imaging of luminescent materials. More particularly, the invention relates to a portable imaging apparatus which can be readily transported by an individual .
  • the apparatus features a system of collapsible or foldable elements which allow it to be stored in a compact configuration when not in use.
  • the apparatus is contained within a case which when opened allows the apparatus to be placed into a use configuration by unfolding or otherwise extending the elements of the apparatus .
  • the apparatus is useful for imaging a variety of materials and samples in life science applications.
  • the form of the luminescence can be light produced by a chemical or biochemical reaction, i.e. chemiluminescence or bioluminescence .
  • Another form is fluorescence or phosphorescence whereby a test sample or a substance is exposed to light of a first wavelength and light is emitted at a second wavelength.
  • Optical filters which only transmit light of a certain wavelength range are used to discriminate emitted light.
  • analyses make use of luminescent endpoints . Southern, northern and western blot assays can be performed by chemiluminescent visualization of bands on the blotting membrane. Microtiter plates, tubes and other types of samples having various shapes and dimensions are also imaged in different types of analyses.
  • Such devices typically use digital means for image capture so that image information can be readily stored, analyzed on computers and displayed on their associated output devices.
  • Charge-coupled device (CCD) camera systems are specially common.
  • Several dedicated, computer- controlled digital imaging systems are commercially available including the EAGLE EYE (Stratagene, La Jolla, California) , CHEMI IMAGER (Alpha Innotech, San Leandro, California ) , BERTHOLD NIGHT OWL (Perkin-Elmer Life Sciences-Wallac, Turku, Finland) , KODAK Image Station 440 CF (Eastman Kodak, Rochester, New York) , NUCLEOVISION 920 (Nucleotech Corp. , San Mateo, California), CHEMI, BIOCHEMI, OPTICHEMI SYSTEMS (UVP, Upland, California) , CCDBIO
  • the relative position of the components defining the optical path are essentially fixed within a rigid cabinet.
  • the present invention addresses this need by providing a compact device which fits inside of a carrying case and is expandable to a configuration for use.
  • the present invention provides a compact, easily portable apparatus for imaging luminescent objects.
  • the apparatus is contained within a carrying case which can be carried and transported by hand.
  • the apparatus is constructed so as to be placed into a first storage configuration when the case is closed and then expanded into a use configuration when the case is opened by unfolding and otherwise expanding the elements of the apparatus .
  • the present invention provides an apparatus for imaging a luminescent object placed within the apparatus comprising a photon detection device, a dark chamber and a sample holder, wherein the apparatus is adapted for storage within a carrying case wherein the apparatus has a closed configuration for storage and an open configuration for use.
  • the apparatus preferably has at least one dimension which is larger when in the open configuration than any dimension of the closed carrying case. In a preferred embodiment the apparatus has at least one dimension which is larger when in the open configuration than the corresponding dimension of the closed carrying case.
  • the apparatus further comprises an expandable enclosure element for providing a dark chamber and electronic means for producing electrical output signals coupled to the photon detection device.
  • Figure 1 is a side view of an apparatus in accordance with the present invention in the open configuration for use but with the flexible bellows not extended.
  • Figure 4A is a photograph of the apparatus in the open configuration for use but with the flexible bellows not extended.
  • Figure 4B is a photograph of the apparatus in the open configuration for use with the flexible bellows extended.
  • Figure 5 is a diagram of the underside of the top support plate showing the two L-brackets for engaging a bellows flange and a central hole for accommodating a camera lens .
  • Figure 10 is a digital image of a membrane from a dot blot experiment acquired using an apparatus of the present device .
  • Figure 11 is a digital image of a membrane from a western blot experiment acquired using an apparatus of the present invention.
  • the blot depicts the chemiluminescent detection of the protein ⁇ -galactosidase on nitrocellulose membrane with a horseradish peroxidase-labeled secondary antibody and Lumigen PS-3 che iluminescent substrate.
  • Figure 12 is a digital image of a membrane from a western blot experiment acquired using an apparatus of the present inventio .
  • the blot depicts the chemiluminescent detection of the protein ⁇ -galactosidase on PVDF membrane with a horseradish peroxidase-labeled secondary antibody and Lumigen PS-3 chemiluminescent substrate.
  • Photon detection device is typically a camera and preferably a digital camera such as a CCD camera. Alternately a photomultiplier tube could be used for sensing and detecting photons of light.
  • the CCD camera consists of a camera head, mating plate and camera lens. CCD camera heads of varying size, resolution (pixel size) number of pixels and A/D conversion bit depth and spectral sensitivity are available and can be used in the present invention.
  • the wavelength sensitivity of the device chosen is governed by the color of light emitted by the luminescent sample. Camera systems employing CCD chip detection are preferable because of the availability of different chips exhibiting sensitivity to a wide range of wavelengths extending from ultraviolet to near infrared.
  • the expandable enclosure element provides a dark environment in which the object to be imaged is placed for imaging.
  • the expandable enclosure element surrounds and encloses at one of its ends at least the outer portion, e.g. a lens, of the photon detection device and can surround the entire photon detection device.
  • the expandable enclosure element surrounds and encloses an area containing the sample by attaching to a surface of the sample holder.
  • the seal is made by conventional means such as clamping or by means of fasteners but in a manner to seal against light leaks .
  • Optional light baffling elements can be used, for example by attachment to or contact with the sample holder, to aid in rejecting stray light.
  • Suitable photographic bellows are manufactured by Toyo Photo Products and are available from photographic product suppliers.
  • a tray which could be designed and fabricated to slide in and out of a small tray holder or cabinet.
  • the plate or tray should have a surface area large enough to accommodate the types of samples which will typically be imaged. In life science applications this includes such objects as blotting membranes and microtiter plates .
  • a mating plate consisted of 1/4" thick round aluminum plate, 4" in diameter and is shown in Figure 7 in bottom and cross sectional views.
  • the mating plate is used to mount a Santa Barbara Instrument Group ST-7E camera head to the hinged top support plate of the portable camera assembly.
  • the mating plate has a hole bored through and tapped in its center to accept standard C-mount threaded lenses. A circular area approximately 2" in diameter near the center hole was thinned to approximately 1/8".
  • the mating plate was additionally bored through to accept countersunk machine screws which attach the camera head to the mating plate. The spacing of the mating plate holes is determined by the preexisting holes in the camera head.
  • the mating plate was also bored and tapped to accept countersunk machine screws which allow it to be attached to the top side of the hinged top support plate of the portable camera assembly.
  • a Toyo 8" x 10" standard photographic bellows, model 180-708 was mounted to the bottom side of the top support plate ( Figure 6) .
  • the bellows is 6" x 6" on the top end and 8" x 10" on the bottom end.
  • a flange on the small (top) end slides into and is held in place on opposite sides by the two L-brackets mounted to the under side of the top support plate.
  • a Santa Barbara Instrument Group Model ST-7E CCD Camera was mounted onto the mating plate by machine screws .
  • the camera contains a 765 x 510 pixel thermoelectrically cooled CCD chip. Pixel size is 9 x 9 ⁇ m making a total area of 6.9 x 4.6 mm.
  • the camera has a 16 bit A/D converter with a 30 KHz digitization rate.
  • a SCSI adaptor with power supply was provided for data transfer to an Apple Macintosh computer.
  • the camera was fitted with a 50 mm C-mount lens (JML Optical, New York, NY) threaded into the mating plate. Other lenses could be easily interchanged.
  • Figure 8 depicts cross section and top views of the assembly of the CCD camera head, mating plate, top support plate and camera lens .
  • a removable prop rod was fabricated from 1/2" aluminum round rod. Use of the prop rod provided extra stability. Both ends of the rod were drilled and tapped to accept #10 threaded rod. The length was approximately 19". The rod is placed between the top support plate and bottom sample holder plate when the apparatus is placed in the open configuration for use. Thumb nuts (#10 thread) tightened the prop rod to these top and bottom hinged plates.
  • Removable hanging straps were attached to opposite sides of the top face of the top support plate.
  • the straps project down from the top support plate approximately 3" and have a lip at the end which hold the bottom (larger) end of the bellows in an nonextended or open position when desired.
  • the apparatus described above was connected to a Macintosh PowerBook laptop computer (Apple Computer, Cupertino, California) by means of a parallel-SCSI converter with associated power supply.
  • a block diagram of the electronic system is depicted in Figure 9.
  • the operation of the camera is software controlled by a program supplied with the camera head by Santa Barbara Instruments Group (CCDOPS) .
  • CCDOPS Santa Barbara Instruments Group
  • the camera head was cooled to 25 °C below ambient temperature via software control . Exposure time and resolution level were specified and exposure made. Operation of the software is described in detail in the software manual.
  • Digoxigenin-labeled DNA (pBR328) , anti digoxigenin- alkaline phosphatase conjugate and positively charged nylon membrane were obtained from Boehringer-Mannheim (Indianapolis, Indiana). DNA dilutions (10, 3, 1, 0.3, 0.1, 0.03, 0.01 pg) were dot blotted onto nylon membranes, blocked, washed and reacted with antibody-AP conjugate according to the manufacturer's protocol. Blots were washed and blocked using customary procedures and then reacted with anti digoxigenin-AP conjugate. After a final wash, excess buffer was drained off and blots soaked in detection reagent (Lumigen CCD, Lumigen, Southfield, Michigan) . Excess reagent was drained off, the blots placed between transparent sheets and placed on the sample holder of the CCD camera imaging apparatus. Figure 12 depicts a 30 s exposure which detected all seven spots .

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Transforming Light Signals Into Electric Signals (AREA)
  • Exposure Control For Cameras (AREA)
  • Telescopes (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

L'invention concerne un appareil compact pour l'imagerie numérique de matières luminescentes. Ledit appareil peut être transporté facilement par une personne et comporte un système d'éléments repliables permettant son rangement selon une configuration compacte lorsqu'il n'est pas utilisé. Ledit appareil est contenu dans un boîtier qui permet, lorsqu'il est ouvert, de placer l'appareil en position d'utilisation, par le déploiement ou l'extension d'une autre manière des éléments de l'appareil. Ledit appareil est utile pour l'imagerie d'une grande variété de matières et d'échantillons dans des applications des sciences de la vie.
PCT/US2001/010823 2000-05-18 2001-05-18 Dispositif d'imagerie optique compact WO2001089219A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US10/276,717 US20040051808A1 (en) 2001-05-18 2001-05-18 Compact optical imaging device
AU2001261012A AU2001261012A1 (en) 2000-05-18 2001-05-18 Compact optical imaging device

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US20506900P 2000-05-18 2000-05-18
US60/205,069 2000-05-18

Publications (1)

Publication Number Publication Date
WO2001089219A1 true WO2001089219A1 (fr) 2001-11-22

Family

ID=22760655

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010823 WO2001089219A1 (fr) 2000-05-18 2001-05-18 Dispositif d'imagerie optique compact

Country Status (2)

Country Link
AU (1) AU2001261012A1 (fr)
WO (1) WO2001089219A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4095874A (en) * 1975-10-06 1978-06-20 Wallace Robert B Hand-held microscope
US4568148A (en) * 1983-11-03 1986-02-04 Onanian Richard A Hand-held collapsible microscope system
US5062697A (en) * 1990-08-08 1991-11-05 Mitchell Phillip R Portable microscope apparatus
US5892539A (en) * 1995-11-08 1999-04-06 Alpha Innotech Corporation Portable emission microscope workstation for failure analysis

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4095874A (en) * 1975-10-06 1978-06-20 Wallace Robert B Hand-held microscope
US4568148A (en) * 1983-11-03 1986-02-04 Onanian Richard A Hand-held collapsible microscope system
US5062697A (en) * 1990-08-08 1991-11-05 Mitchell Phillip R Portable microscope apparatus
US5892539A (en) * 1995-11-08 1999-04-06 Alpha Innotech Corporation Portable emission microscope workstation for failure analysis

Also Published As

Publication number Publication date
AU2001261012A1 (en) 2001-11-26

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