WO2001085786A2 - Human histamine h4 receptor - Google Patents
Human histamine h4 receptor Download PDFInfo
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- WO2001085786A2 WO2001085786A2 PCT/US2001/014527 US0114527W WO0185786A2 WO 2001085786 A2 WO2001085786 A2 WO 2001085786A2 US 0114527 W US0114527 W US 0114527W WO 0185786 A2 WO0185786 A2 WO 0185786A2
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- receptor
- sequence
- nucleic acid
- receptor protein
- protein
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/72—Receptors; Cell surface antigens; Cell surface determinants for hormones
- C07K14/723—G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
Definitions
- the present invention discloses the identification of a novel histamine receptor, termed H 4 .
- H H 2 histamine produces numerous physiological effects in the body through interaction with one of three different cell surface receptors, classified as H H 2 , and H 3 . These receptors belong to the guanine nucleotide binding protein coupled receptor (G-protein coupled receptors, GPCR) class.
- G-protein coupled receptors GPCR
- Stimulation of histamine H- receptors produces symptoms that are typically associated with physiological responses to allergic stimuli (Ash and Schild, Br. J. Pharmacol.
- H- antagonists such as, for example, diphenhydramine.
- H* antagonists are generally defined as "classical antihistamines". Classical antihistamines are the active ingredient in most over-the-counter allergy medications. Pharmacological studies indicate that agonist activation of these receptors stimulates the inositol phosphate pathway, and thus stimulates formation of inositol triphosphate (IP 3 ) and diacylglycerol (DAG).
- IP 3 inositol triphosphate
- DAG diacylglycerol
- Histamine H 2 receptors have been shown to play a role in gastric acid secretions (Black et al, Nature 1972, 236:385).
- Histamine H 2 receptor antagonists such as, for example, cimetidine and ranitidine, are often the active ingredient in over-the-counter and prescription drugs that are used to treat duodenal ulcers, gastric ulcers, heartburn, indigestion, and other disorders of the gastrointestinal tract.
- Activation of histamine H 2 stimulates adenylyl cyclase activity and stimulates formation of c AMP.
- Histamine H 3 receptors are a relatively new member of the histamine receptor class. These receptors were originally described as histamine autoreceptors.
- H 3 receptors were shown to be located on presynaptic histamine nerve terminals and to control the rate of histamine synthesis and release in the brain (Arrang etal, Nature 1983, 302:832). Recent studies indicate that H 3 receptors also are present on non-histamine presynaptic nerve terminals. H 3 receptors may modulate the synthesis and release of other neurotransmitters such as dopamine, serotonin, acetylcholine, and norepinephrine. H 3 receptors also have been found in peripheral tissues. Histamine activation of H 3 receptor inhibits adenylyl cyclase activity and formation of cAMP.
- H 3 receptor subtypes Leurs et al JPET 1996, 276:1009-1015; Gumming and Gjede, Brain Res. 1994, 641:203-207; Calpham and Kilpatrick, Br. J. Pharmacol 1996, 107:919-923; Schworer et al Naunyn-Schmiedeberg's Arch. Pharmacol. 1994, 350:375-379; Schkicker et al, Naunyn- Schmiedeberg's Arch. Pharmacol. 1996, 353:482-488).
- the present invention contemplates an isolated histamine H 4 receptor protein having an amino acid sequence at least 51 % identical or comprising at least 10 contiguous amino acids from the sequence depicted in SEQ ID NO:2.
- the receptor protein upon binding histamine or a histamine agonist the receptor protein inhibits second messenger formation.
- the second messenger is cAMP.
- the present application also discloses an isolated H 4 receptor protein having an amino acid sequence with at least 95% sequence identity to human H 4 receptor protein having an amino acid sequence as depicted in SEQ ID NO: 2.
- the protein is a human H 4 receptor protein, e.g. , having an amino acid sequence as depicted in SEQ ID NO:2 or an allelic variant of that sequence.
- nucleic acids e.g., cDNAs encoding the H 4 receptor proteins as discussed above.
- an isolated nucleic acid encoding an H 4 receptor protein which nucleic acid hybridizes under stringent conditions to a nucleic acid having a sequence of at least 20 nucleotides identical to a corresponding nucleotide sequence ofthe same number of bases in SEQ ID NO:l or its complement.
- the isolated nucleic acids encoding H 4 receptors can be part of vectors, e.g., for cloning, expression, and/or expansion.
- An expression vector comprises the nucleic acid encoding the H 4 receptor protein operably associated with an expression control sequence.
- the invention further provides host cells and non-human transgenic animals containing such an expressible vector, and methods for producing an H 4 receptor polypeptide using such host cells.
- the invention provides an isolated nucleic acid, such as a primer or probe, of at least 10 bases having a nucleotide sequence identical to a corresponding nucleotide sequence ofthe same number of bases in SEQ ID NO:l, or its complement.
- the invention also provides an antibody that specifically binds an H 4 receptor.
- the present invention further contemplates a method for detecting expression of H 4 receptor, which method comprises detecting mRNA encoding H 4 receptor in a sample from a cell suspected of expressing H 4 receptor or detecting the H 4 receptor protein with an antibody ofthe invention.
- the present invention also contemplates an assay system for identifying H 4 receptor ligands.
- the assay system comprises a- sufficient number of transformed host cells to be able to detect an alteration in second messenger accumulation.
- the second messenger is cAMP.
- the present invention also contemplates a method for identifying a test compound that antagonizes or agonizes histamine H 4 receptors.
- the method comprises detecting an alteration in the level of a second messenger in the assay system contacted with the test compound.
- an increase in the level ofthe second messenger indicates that the test compound antagonizes the H 4 receptor.
- a decrease in the level of the second messenger indicates that the test compound agonizes the H 4 receptor.
- the present invention also discloses an isolated nucleic acid that specifically hybridizes under highly stringent conditions to the complement ofthe sequence depicted in SEQ ID NO:l, where the nucleic acid encodes a histamine H 4 receptor protein.
- FIGS. 2A, 2B, and 2C (A) Agonist assay, Square: histamine; triangle: R- ⁇ - methylhistamine; inverted triangle: clobenpropit; diamond: thioperamide. (B) Antagonist assay.
- Figure 4 H 4 expression in from 6 human T cell clones derived from a single human donor. Three of these clones were CD4+ cell clones (RG4.3B, RG4.3A, and RG4.3), two were CD8+ cell clones (RG8.1C and RG8.1A), and one was an NKT cell clone (RG1).
- FIG. H 4 expression in bulk populations of CD4+ and CD8+ T cells isolated from three different individuals.
- B-CD8 and B-CD4 were CD8+ and CD4+ cells, respectively, obtained from individual 1.
- L-CD8 and L-CD4 were CD8+ and CD4+ cells, respectively, obtained from individual 2.
- U-CD8 and U-CD4 were CD8+ and CD4+ cells, respectively, obtained from individual 3.
- the present invention is based, in part, on discovery of a no vel histamine receptor, which has been termed H 4 .
- the new histamine receptor was cloned from a human heart library using H 3 specific primers, but proved to be a new histamine receptor.
- Yeast and human endothelial kidney (HEK) cells were transformed with a human H 4 expression vector.
- Transformed cells in multi- ell plates were treated with test compounds, and regulation ofthe intracellular second messenger cyclic adenosine monophosphate (cAMP) formation was determined. Modulation of cAMP formation is ligand and concentration dependent.
- cAMP second messenger cyclic adenosine monophosphate
- H 4 shows homology to known G- protein coupled receptors. Specifically, the H 4 protein shows homology to biogenic-amine G- protein coupled receptors. As in other biogenic amine receptors, the present protein contains conserved aspartate residues within transmembrane (TM) domains 2 and 3 (at positions 61 and
- a DY motif (D94 and Y95) in TM3 is found only in histamine and muscarinic receptors.
- a DRY sequence motif that exists at the cytoplasmic interface of TM3 of all GPCRs also can be found in the present sequence (amino acids 111- 113). Also present are two conserved cysteine residues in the first and second extracellular loops (position 88 and 164), which are predicted to form a disulfide bond; two conserved tryptophan residues in TM4 and TM6 (positions 140 and
- the putative TM domains are shown in Figure 1.
- H 4 shares sequence identity with human H 3 receptors. Sequence comparison indicates an overall sequence identity of about 44% and overall sequence similarity of about 51%.
- the present invention also contemplates an assay method and system for identifying selective H 4 receptor ligands. The method involves detecting binding of a test compound to isolated cell membranes containing the histamine H 4 receptor.
- the assay system comprises transformed host cells that express H 4 receptors, where the number of cells in the assay system is sufficient to detect an alteration in second messenger accumulation.
- the test system also includes an appropriate cell culture medium to permit cell growth and viability, and preferably tissue culture plates or arrays containing the host cells in cell culture medium.
- the second messenger that is detected is cAMP.
- the receptor is a human receptor.
- the invention also discloses a method for identifying a test compound that antagonizes or agonizes histamine H 4 receptors.
- the method comprises detecting an increase
- the present invention advantageously provides H 4 protein, including fragments, derivatives, and analogs of H 4 ; H 4 nucleic acids, including oligonucleotide primers and probes, and H 4 regulatory sequences (especially an H 4 primer and splice sites with introns);
- H 4 -specific antibodies and related methods of using these materials to detect the presence of H 4 proteins or nucleic acids, H 4 binding partners, and in screens for agonists and antagonists of H 4 .
- an isolated nucleic acid means that the referenced material is removed from the environment in which it is normally found.
- an isolated biological material can be free of cellular components, z ' .e., components of the cells in which the material is found or produced in nature.
- an isolated nucleic acid includes a
- an isolated nucleic acid is preferably excised from the chromosome in which it may be found, and more preferably is no longer joined to non-regulatory, non-coding regions, or to other genes, located upstream or downstream of the gene contained by the isolated nucleic acid molecule when found in the chromosome.
- the isolated nucleic acid lacks one or more introns. Isolated nucleic acid molecules include sequences inserted into plasmids, cosmids, artificial chromosomes, and the like.
- a recombinant nucleic acid is an isolated nucleic acid.
- An isolated protein may be associated with other proteins or nucleic acids, or both, with which it associates in the cell, or with cellular membranes if it is a membrane-associated protein.
- a protein expressed from a vector in a cell, particularly a cell, in which the protein is normally not expressed is also a regarded as isolated.
- An isolated organelle, cell, or tissue is removed from the anatomical site in which it is found in a cell or an organism.
- An isolated material may be, but need not be, purified.
- purified refers to material that has been isolated under conditions that reduce or eliminate the presence of unrelated materials, i.e., contaminants, including native materials from which the material is obtained.
- a purified protein is preferably substantially free of other proteins or nucleic acids with which it is associated in a cell;
- a purified nucleic acid molecule is preferably substantially free of proteins or other unrelated nucleic acid molecules with which it can be found within a cell.
- substantially free is used operationally, in the context of analytical testing of the material.
- purified material substantially free of contaminants is at least 50% pure; more preferably, at least 90% pure; and more preferably still at least 99% pure. Purity can be evaluated by chromatography, gel electrophoresis, immunoassay, composition analysis, biological assay, and other methods known in the art.
- nucleic acids can be purified by precipitation, chromatography (including preparative solid phase chromatography, oligonucleotide hybridization, and triple helix chromatography), ultracentrifugation, and other means.
- Polypeptides and proteins can be purified by various methods including, without limitation, preparative disc-gel electrophoresis, isoelectric focusing, HPLC, reversed-phase HPLC, gel filtration, ion exchange and partition chromatography, precipitation and salting-out chromatography, extraction, and countercurrent distribution.
- the polypeptide in a recombinant system in which the protein contains an additional sequence tag that facilitates purification, such as, but not limited to, a polyhistidine sequence, or a sequence that specifically binds to an antibody, such as FLAG and GST.
- the polypeptide can then be purified from a crude lysate of the host cell by chromatography on an appropriate solid-phase matrix.
- antibodies produced against the protein or against peptides derived therefrom can be used as purification reagents.
- Cells can be purified by various techniques, including centrifugation, matrix separation (e.g., nylon wool separation), panning and other immunoselection techniques, depletion (e.g., complement depletion of contaminating cells), and cell sorting (e.g., fluorescence activated cell sorting [FACS]). Other purification methods are possible.
- a purified material may contain less than about 50%), preferably less than about 75%), and most preferably less than about 90%, of the cellular components with which it was originally associated. The "substantially pure" indicates the highest degree of purity which can be achieved using conventional purification techniques known in the art.
- the term "about” or “approximately” means within a scientifically acceptable error range for a given value relative to the precision with which the value is or can be measured, e.g., within 20% > , preferably within 10%, and more preferably within 5%> of a given value or range.
- the term can mean within an order of magnitude, preferably within 5 -fold and more preferably within 2-fold of a given value.
- sample refers to a biological material which can be tested for the presence of H 4 protein or H 4 nucleic acids.
- samples can be obtained from animal subjects, such as humans and non-human animals, and include tissue, especially muscle, biopsies, blood and blood products; plural effusions; cerebrospinal fluid (CSF); ascites fluid; and cell culture.
- Non-human animals include, without limitation, laboratory animals such as mice, rats, rabbits, hamsters, guinea pigs, etc.; domestic animals such as dogs and cats; and, farm animals such as sheep, goats, pigs, horses, and cows.
- ligand refers to a compound that recognizes and binds to a receptor binding site. In a specific embodiment, the ligand binds to the histamine H 4 receptors of the invention. Upon binding to the receptor, the ligand may produce agonist or antagonist functional effects.
- agonist refers to a ligand that binds to the receptor and produces a functional effect similar to that produced by the endogenous ligand for the receptor.
- the agonist at the histamine H 4 receptor produces an effect similar to that produced by histamine, the endogenous ligand (histamine) for the H 4 receptor.
- examples of such agonists include, but are not limited to, R- ⁇ -methyl histamine and imetit.
- antagonist refers to a ligand that binds to the receptor and blocks a functional effect produced by an agonist for the receptor or the endogenous ligand ofthe receptor.
- Examples of such antagonists include, but are not limited to, thioperamide.
- selective refers to the ability of a histamine H 4 agonist or antagonist to elicit a response from the H 4 receptor while eliciting minimal responses from another receptor.
- a selective H 4 agonist may be a potent agonist for the H 4 receptor while agonizing another receptor, such as another G-protein coupled receptor and particularly another histamine receptor, poorly or not at all.
- the term "ability to elicit a response” refers to the ability of a H 4 agonist or antagonist ligand to agonize or antagonize H receptor activity.
- transformed cell refers to a modified host cell that expresses a functional H 4 receptor expressed from a vector encoding the histamine receptor.
- a "functional histamine receptor” is a receptor that binds histamine or H 4 agonists and transduces a signal upon such binding.
- the H 4 receptor is a human H 4 .
- the signal that is transduced is accumulation of a second messenger, preferably cAMP.
- Histamine H 4 receptors may be derived from a variety of sources, including mammal, e.g., human, bovine, porcine, and canine; and avian.
- the cells of the invention are particularly suitable for an assay system for histamine H 4 receptor ligands that modulate second messenger accumulation.
- An "assay system" for histamine H 4 receptor ligands that modulate second messenger accumulation.
- ' is one or more collections of such cells, e.g., in a microwell plate or some other culture system.
- the number of cells in a single assay system is sufficient to express a detectable amount of the regulated second messenger accumulation at least under conditions of maximum second messenger accumulation.
- a "second messenger” is an intracellular molecule or ion, where formation and/or accumulation ofthe second messenger is regulated by activation of cellular membranes.
- cellular membranes contain G-protein coupled receptor, ion channels, and tyroinse kinase receptors.
- the cellular membrane is a G-protein coupled receptor, preferably a histamine H 4 receptor.
- the second messenger is one or more of cAMP, cGMP, inositol phosphate, DAG, and ions such as calcium and potassium.
- the second messenger is cAMP.
- test compound or “candidate compound” is any molecule that can be tested for its ability to bind H 4 receptors, and preferably modulate second messenger accumulation through the H 4 receptor, as set forth herein.
- H 4 is a "lead compound" suitable for further testing and development as an H 4 agonist or antagonist.
- the term "provide” refers to supplying the compounds or pharmaceutical compositions of the present invention to cells or to an animal, preferably a human, in any form.
- a prodrug form ofthe compounds may be provided the subj ect, which then is metabolized to the compound in the body.
- H 4 receptor refers to a polypeptide having about 390 amino acids.
- H 4 refers to orthologs and allelic variants, e.g. , a protein having greater than about 50%), preferably greater than 80%, more preferably still greater than 90%, and even more preferably greater than 95% overall sequence identity to SEQ ID NO: 2.
- Allelic variants may differ from 1 to about 5 amino residues from SEQ ID NO:2.
- H 4 has an amino acid sequence as shown in SEQ ID NO: 2.
- H 4 receptors like H 2 and H 3 receptors, modulates adenylyl cyclase activity. Therefore, the receptor modulates accumulation of the intracellular messenger cAMP. Modulation of H 4 receptors may be a treatment for transplanted organ rejection, asthma, allergies, and autoimmune pathologies, such as, but not limited to, multiple sclerosis, type I diabetes, and rheumatoid arthritis. Additionally, CNS functions such as cognitive and memory defects may be treated.
- H 4 fragments, derivatives, and analogs can be characterized by one or more of the characteristics of H 4 protein.
- antibodies in order to develop the specific C-terminal and N-terminal H 4 antibodies, antibodies can be raised against extracellular or cytoplasmic portions of the H 4 protein, preferably or antigenic peptides identified using a hydrophobicity profile or other algorithms.
- Analogs and derivatives of the H 4 receptor of the invention have the same or homologous characteristics of H 4 as set forth above.
- a truncated form of H 4 can be provided. Such a truncated form includes H 4 with a either an N-terminal, C-terminal, or internal deletion.
- the derivative is functionally active, i.e., capable of exhibiting one or more functional activities associated with a full-length, wild-type H 4 of the invention.
- Such functions include, but are not limited to, inhibition of adenylyl cyclase activity and cAMP formation.
- a H 4 chimeric fusion protein can be prepared in which the H 4 portion of the fusion protein has one or more characteristics of H 4 .
- Such fusion proteins include fusions ofthe H 4 receptor with a marker polypeptide, such as FLAG, a histidine tag, a myc tag, or glutathione-S-transferase (GST).
- the H 4 receptor can be fused with an expression-related peptide, such as yeast ⁇ -mating factor, a heterogeneous signal peptide, or a peptide that renders the protein more stable upon expression.
- H 4 can also be fused with a unique phosphorylation site for labeling.
- H 4 analogs can be made by altering encoding nucleic acid sequences by substitutions, additions or deletions that provide for functionally similar molecules, i.e., molecules that perform one or more H 4 functions.
- an analog of H 4 is a sequence-conservative variant of H 4 .
- an analog of H 4 is a function- conservative variant.
- an analog of H 4 is an allelic variant or a homologous variant from another species.
- human variants of H 4 are described.
- H 4 derivatives include, but are by no means limited to, phosphorylated H 4 , glycosylated H 4 , methylated H 4 , and other H 4 proteins that are otherwise chemically modified.
- H 4 derivatives also include labeled variants, e.g., radio-labeled with iodine (or, as pointed out above, phosphorous); a detectable molecule, such as but by no means limited to biotin, a chelating group complexed with a metal ion, a chromophore " br fluorophore, a gold colloid, or a particle such as a latex bead; or attached to a water soluble polymer.
- the present invention contemplates analysis and isolation of a gene encoding a functional or mutant H 4 , including a full length, or naturally occurring form of H 4 , and any antigenic fragments thereof from any source, preferably human. It further contemplates expression of functional or mutant H 4 protein for evaluation, diagnosis, or therapy.
- PCR polymerase chain reaction
- sequence-specific oligonucleotides refers to related sets of oligonucleotides that can be used to detect allelic variations or mutations in the H 4 gene.
- nucleic acid molecules herein may be flanked by natural regulatory (expression control) sequences, or may be associated with heterologous sequences, including promoters, internal ribosome entry sites (IRES) and other ribosome binding site sequences, enhancers, response elements, suppressors, signal sequences, polyadenylation sequences, introns, 5'- and 3'- non-coding regions, and the like.
- the nucleic acids may also be modified by many means known in the art.
- Non-limiting examples of such modifications include methylation, "caps”, substitution of one or more ofthe naturally occurring nucleotides with an analog, and internucleotide modifications such as, for example, those with uncharged linkages (e.g., methyl phosphonates, phosphotriesters, phosphoroamidates, carbamates, etc.) and with charged linkages (e.g., phosphorothioates, phosphorodithioates, etc.).
- uncharged linkages e.g., methyl phosphonates, phosphotriesters, phosphoroamidates, carbamates, etc.
- charged linkages e.g., phosphorothioates, phosphorodithioates, etc.
- Polynucleotides may contain one or more additional covalently linked moieties, such as, for example, proteins (e.g., nucleases, toxins, antibodies, signal peptides, poly-L-lysine, etc.), intercalators (e.g., acridine, psoralen, etc.), chelators (e.g., metals, radioactive metals, iron, oxidative metals, etc.), and alkylators.
- the polynucleotides may be derivatized by formation of a methyl or ethyl phosphotriester or an alkyl phosphoramidate linkage.
- polynucleotides herein may also be modified with a label capable of providing a detectable signal, either directly or indirectly.
- exemplary labels include radioisotopes, fluorescent molecules, biotin, and the like.
- RNA, polypeptide, protein, or enzyme is a nucleotide sequence that, when expressed, results in ' the production of that RNA, polypeptide, protein, or enzyme, i.e., the nucleotide sequence encodes an amino acid sequence for that polypeptide, protein or enzyme.
- a coding sequence for a protein may include a start codon (usually ATG) and a stop codon.
- gene also called a "structural gene” means a DNA sequence that codes for or corresponds to a particular sequence of amino acids which comprise all or part of one or more proteins or enzymes, and may or may not include introns and regulatory DNA sequences, such as promoter sequences, 5 '-untranslated region, or 3'-untranslated region which affect for example the conditions under which the gene is expressed.
- promoter sequence is a DNA regulatory region capable of binding RNA polymerase in a cell and initiating transcription of a downstream (3' direction) coding sequence.
- the promoter sequence is bounded at its 3' terminus by the transcription initiation site and extends upstream (5' direction) to include the minimum number of bases or elements necessary to initiate transcription at levels detectable above background. Within the promoter sequence will be found a transcription initiation site
- the present invention includes the H 4 receptor gene promoter found in the genome, which can be operatively associated with a H 4 coding sequence with a heterologous coding sequence.
- the term "host cell” means any cell of any organism that is selected, modified, transformed, grown, or used or manipulated in any way, for the production of a substance by the cell, for example the expression by the cell of a gene, a DNA or RNA sequence, a protein or an enzyme. Host cells can further be used for screening or other assays, as described infra.
- a coding sequence is "under the control of or “operatively associated with” transcriptional and translational control sequences in a cell when RNA polymerase transcribes the coding sequence into mRNA, which is then trans-RNA spliced (if it contains introns) and translated, in the case of mRNA, into the protein encoded by the coding sequence.
- express and expression mean allowing or causing the information in a gene or DNA sequence to become manifest, for example producing a protein by activating the cellular functions involved in transcription and translation of a corresponding gene or DNA sequence.
- a DNA sequence is expressed in or by a cell to form an "expression product” such as a protein.
- the expression product itself e.g., the resulting protein, may also be said to be “expressed” by the cell.
- An expression product can be characterized as intracellular, extracellular, transmembrane, or secreted.
- intracellular means something that is inside a cell.
- extracellular means something that is outside a cell.
- the H 4 receptor is a seven transmembrane protein with intracellular and extracellular domains.
- transfection means the introduction of a foreign nucleic acid into a cell.
- transformation means the introduction of a "foreign” (i.e., extrinsic or extracellular) gene, DNA or RNA sequence to a host cell, so that the host cell will express the introduced gene or sequence to produce a desired substance, typically a protein or enzyme coded by the introduced gene or sequence.
- the introduced gene or sequence may also be called a “cloned” or “foreign” gene or sequence, may include regulatory or control sequences, such as start, stop, promoter, signal, secretion, or other sequences used by a cell's genetic machinery.
- the gene or sequence may include nonfunctional sequences or sequences with no known function.
- a host cell that receives and expresses introduced DNA or RNA has been "transformed” and is a "transformant” or a “clone.”
- the DNA or RNA introduced to a host cell can come from any source, including cells ofthe same genus or species as the host cell, or cells of a different genus or species.
- the terms “vector”, “cloning vector” and “expression vector” mean the vehicle by which a DNA or RNA sequence (e.g., a foreign gene) can be introduced into a host cell, so as to transform the host and promote expression (e.g., transcription and translation) of the introduced sequence.
- Vectors include plasmids, phages, viruses, etc.; they are discussed in greater detail below.
- Vectors typically comprise the DNA of a transmissible agent, into which foreign
- DNA is inserted.
- a common way to insert one segment of DNA into another segment of DNA involves the use of enzymes called restriction enzymes that cleave DNA at specific sites (specific groups of nucleotides) called restriction sites.
- restriction enzymes that cleave DNA at specific sites (specific groups of nucleotides) called restriction sites.
- a "cassette” refers to a DNA coding sequence or segment of DNA that codes for an expression product that can be inserted into a vector at defined restriction sites.
- the cassette restriction sites are designed to ensure insertion ofthe cassette in the proper reading frame.
- foreign DNA is inserted at one or more restriction sites ofthe vector DNA, and then is carried by the vector into a host cell along with the transmissible vector DNA.
- a segment or sequence of DNA having inserted or added DNA, such as an expression vector can also be called a "DNA construct.”
- a common type of vector is a "plasmid", which generally is a self-contained molecule of double-stranded DNA, usually of bacterial origin, that can readily accept additional (foreign) DNA and which can readily introduced into a suitable host cell.
- a plasmid vector often contains coding DNA and promoter DNA and has one or more restriction sites suitable for inserting foreign DNA.
- Coding DNA is a DNA sequence that encodes a particular amino acid sequence for a particular protein or enzyme.
- Promoter DNA is a DNA sequence which initiates, regulates, or otherwise mediates or controls the expression ofthe coding DNA.
- Promoter DNA and coding DNA may be from the same gene or from different genes, and may be from the same or different organisms.
- a large number of vectors, including plasmid and fungal vectors, have been described for replication and/or expression in a variety of eukaryotic and prokaryotic hosts.
- Non-limiting examples include pKK plasmids (Clonetech), pUC plasmids, pET plasmids (Novagen, Inc., Madison, WI), pRSET or pREP plasmids (Invitrogen, San Diego, CA), or pMAL plasmids (New England
- Recombinant cloning vectors will often include one or more replication systems for cloning or expression, one or more markers for selection in the host, e.g., antibiotic resistance, and one or more expression cassettes.
- expression system means a host cell and compatible vector under suitable conditions, e.g., for the expression of a protein coded for by foreign DNA carried by the vector and introduced to the host cell. Common expression systems include E. coli host cells and plasmid vectors, insect host cells and Baculovirus vectors, and mammalian host cells and vectors.
- heterologous refers to a combination of elements not naturally occurring.
- heterologous DNA refers to DNA not naturally located in the cell, or in a chromosomal site ofthe cell.
- the heterologous DNA includes a gene foreign to the cell.
- a heterologous expression regulatory element is such an element operatively associated with a different gene than the one it is operatively associated with in nature.
- an H 4 gene is heterologous to the vector DNA in which it is inserted for cloning or expression, and it is heterologous to a host cell containing such a vector, in which it is expressed, e.g., a HEK cell.
- mutant and mutant mean any detectable change in genetic material, e.g., DNA, or any process, mechanism, or result of such a change. This includes gene mutations, in which the structure (e.g., DNA sequence) of a gene is altered, any gene or DNA arising from any mutation process, and any expression product (e.g., protein or enzyme) expressed by a modified gene or DNA sequence.
- variant may also be used to indicate a modified or altered gene, DNA sequence, enzyme, cell, etc., i.e., any kind of mutant.
- Sequence-conservative variants of apolynucleotide sequence are those in which a change of one or more nucleotides in a given codon position results in no alteration in the amino acid encoded at that position.
- “Function-conservative variants” are those in which a given amino acid residue in a protein or enzyme has been changed without altering the overall conformation and function ofthe polypeptide, including, but not limited to, replacement of an amino acid with one having similar properties (such as, for example, polarity, hydrogen bonding potential, acidic, basic, hydrophobic, aromatic, and the like).
- Amino acids with similar properties are well known in the art. For example, arginine, histidine and lysine are hydrophilic-basic amino acids and may be interchangeable. Similarly, isoleucine, a hydrophobic amino acid, may be replaced with leucine, methionine or valine.
- a “function-conservative variant” also includes a polypeptide or enzyme which has at least 60 % ⁇ amino acid identity as determined by BLAST or FASTA algorithms, preferably at least 75%, most preferably at least 85%>, and even more preferably at least 90%o, and which has the same or substantially similar properties or functions as the native or parent protein or enzyme to which it is compared.
- homologous in all its grammatical forms and spelling variations refers to the relationship between proteins that possess a "common evolutionary origin,” including proteins from superfamilies (e.g., the immunoglobulin superfamily) and homologous proteins from different species (e.g., myosin light chain, etc.) (Reeck et al, Cell 1987, 50:667). Such proteins (and their encoding genes) have sequence homology, as reflected by their sequence similarity, whether in terms of percent similarity or the presence of specific residues or motifs at conserved positions.
- sequence similarity in all its grammatical forms refers to the degree of identity or correspondence between nucleic acid or amino acid sequences of proteins that may or may not share a common evolutionary origin (see Reeck et al, supra).
- sequence similarity when modified with an adverb such as "highly,” may refer to sequence similarity and may or may not relate to a common evolutionary origin.
- two DNA sequences are "substantially homologous" or “substantially similar” when at least about 80%>, and most preferably at least about 90 or 95%> of the nucleotides match over the defined length of the DNA sequences, as determined by sequence comparison algorithms, such as BLAST, FASTA, DNA Strider, etc.
- sequence comparison algorithms such as BLAST, FASTA, DNA Strider, etc.
- An example of such a sequence is an allelic or species variant of the specific H 4 gene of the invention.
- Sequences that are substantially homologous can be identified by comparing the sequences using standard software available in sequence data banks, or in a Southern hybridization experiment under, for example, stringent conditions as defined for that particular system.
- two amino acid sequences are "substantially homologous” or “substantially similar” when greater than 80% ofthe amino acids are identical, or greater than about 90%) are similar (functionally identical).
- the similar or homologous sequences are identified by alignment using, for example, the GCG (Genetics Computer Group, Program Manual for the GCG Package, Version 7, Madison, Wisconsin) pileup program, or any ofthe programs described above (BLAST, FASTA, etc)
- GCG Genetics Computer Group, Program Manual for the GCG Package, Version 7, Madison, Wisconsin
- FASTA FASTA, etc
- a nucleic acid molecule is "hybridizable" to another nucleic acid molecule, such as a cDNA, genomic DNA, or RNA, when a single stranded form ofthe nucleic acid molecule can anneal to the other nucleic acid molecule under the appropriate conditions of temperature and solution ionic strength (see Sambrook et al, supra).
- the conditions of temperature and ionic strength determine the "stringency" of the hybridization.
- low stringency hybridization conditions corresponding to a Tm (melting temperature) of 55°C
- Tm melting temperature
- Moderate stringency hybridization conditions correspond to a higher Tm, e.g., 40%> formamide, with 5x or 6x SSC.
- High stringency hybridization conditions correspond to the highest Tm, e.g., 50% formamide, 5x or 6x SSC.
- SSC is a 0.15M NaCl, 0.015M Na-citrate buffer.
- Hybridization requires that the two nucleic acids contain complementary sequences, although depending on the stringency ofthe hybridization, mismatches between bases are possible.
- the appropriate stringency for hybridizing nucleic acids depends on the length of the nucleic acids and the degree of complementation, variables well known in the art. The greater the degree of similarity or homology between two nucleotide sequences, the greater the value of Tm for hybrids of nucleic acids having those sequences.
- the relative stability (corresponding to higher Tm) of nucleic acid hybridizations decreases in the following order: RNA:RNA, DNA:RNA, DNA:DNA. For hybrids of greater than 100 nucleotides in length, equations for calculating Tm have been derived (see Sambrook et al.
- a minimum length for a hybridizable nucleic acid is at least about 10 nucleotides; preferably at least about 15 nucleotides; and more preferably the. length is at least about 20 nucleotides.
- standard hybridization conditions refers to a Tm of 55 °C, and utilizes conditions as set forth above.
- the Tm is 60 °C; in a more preferred embodiment, the Tm is 65 °C.
- “high stringency” refers to hybridization and/or washing conditions at 68 °C in 0.2xSSC, at 42°C in 50%) formamide, 4xSSC, or under conditions that afford levels of hybridization equivalent to those observed under either of these two conditions.
- oligonucleotide refers to a nucleic acid, generally of at least 10, preferably at least 15 , and more preferably at least 20 nucleotides, preferably no more than 100 nucleotides, that is hybridizable to a genomic DNA molecule, a cDNA molecule, or an mRNA molecule encoding a gene, mRNA, cDNA, or other nucleic acid of interest. Oligonucleotides can be labeled, e.g., with 32 P-nucleotides or nucleotides to which a label, such as biotin, has been covalently conjugated.
- a labeled oligonucleotide can be used as a probe to detect the presence of a nucleic acid.
- oligonucleotides (one or both of which may be labeled) can be used as PCR primers, either for cloning full length or a fragment of H 4 , or to detect the presence of nucleic acids encoding H 4 .
- an oligonucleotide of the invention can form a triple helix with a H 4 DNA molecule.
- oligonucleotides are prepared synthetically, preferably on a nucleic acid synthesizer. Accordingly, oligonucleotides can be prepared with non-naturally occurring phosphoester analog bonds, such as thioester bonds, etc.
- the present invention provides antisense nucleic acids (including ribozymes), which may be used to inhibit expression of H 4 ofthe invention. Inhibition of H 4 expression may be desired when upregulation of H 4 receptor expression or excessive inhibition of cAMP formation induces disease states such as, transplant organ rejection; asthma; allergies; autoimmune pathologies, such as, but not limited to, multiple sclerosis, type I diabetes, and rheumatoid arthritis; and CNS functions such as cognitive and memory defects.
- An "antisense nucleic acid” is a single stranded nucleic acid molecule which, on hybridizing under cytoplasmic conditions with complementary bases in an RNA or DNA molecule, inhibits the latter's role. If the RNA is a messenger RNA transcript, the antisense nucleic acid is a countertranscript or mRNA-interfering complementary nucleic acid. As presently used, "antisense” broadly includes
- Antisense nucleic acid molecules can be encoded by a recombinant gene for expression in a cell (e.g., U.S. Patent No. 5,814,500; U.S. Patent No. 5,811,234), or alternatively they can be prepared synthetically (e.g., U.S. Patent No. 5,780,607).
- synthetic oligonucleotides envisioned for this invention include oligonucleotides that contain phosphorothioates, phosphotriesters, methyl phosphonates, short chain alkyl, or cycloalkyl intersugar linkages or short chain heteroatomic or heterocyclic intersugar linkages.
- 5,637,684 describes phosphoramidate and phosphorothioamidate oligomeric compounds.
- oligonucleotides having morpholino backbone structures U.S. Patent No. 5,034,506.
- the phosphodiester backbone of the oligonucleotide may be replaced with a polyamide backbone, the bases being bound directly or indirectly to the aza nitrogen atoms ofthe polyamide backbone (Nielsen et al, Science 254:1497, 1991).
- oligonucleotides may contain substituted sugar moieties comprising one ofthe following at the 2' position: OH, SH, SCH 3 , F, OCN, O(CH 2 ) n NH 2 or O(CH 2 ) n CH 3 where n is from 1 to about 10; CI to CIO lower alkyl, substituted lower alkyl, alkaryl or aralkyl; CI; Br; CN; CF 3 ; OCF 3 ; O-; S-, or N-alkyl; O-, S-, or
- N-alkenyl SOCH 3 ; SO 2 CH 3 ; ONO 2 ; NO 2 ; N 3 ; NH 2 ; heterocycloalkyl; heterocycloalkaryl; aminoalkylamino; polyalkylamino; substitued silyl; a fluorescein moiety; an RNA cleaving group; a reporter group; an intercalator; a group for improving the pharmacokinetic properties of an oligonucleotide; or a group for improving the pharmacodynamic properties of an oligonucleotide, and other substituents having similar properties.
- Oligonucleotides may also have sugar mimetics such as cyclobutyls or other carbocyclics in place of the pentofuranosyl group.
- Nucleotide units having nucleosides other than adenosine, cytidine, guanosine, thymidine and uridine, such as inosine, may be used in an oligonucleotide molecule.
- H 4 Nucleic Acids A gene encoding H 4 , whether genomic DNA or cDNA, can be isolated from any source, particularly from a human cDNA or genomic library. Methods for obtaining H 4 gene are well known in the art, as described above (see, e.g., Sambrook et al, 1989, supra).
- the DNA may be obtained by standard procedures known in the art from cloned DNA (e.g., a DNA "library”), and preferably is obtained from a cDNA library prepared from tissues with high level expression ofthe protein, by chemical synthesis, by cDNA cloning, or by the cloning of genomic
- Clones derived from genomic DNA may contain regulatory and intron DNA regions in addition to coding regions; clones derived from cDNA will not contain intron sequences. Whatever the source, the gene should be molecularly cloned into a suitable vector for propagation ofthe gene. Identification ofthe specific DNA fragment containing the desired H 4 gene may be accomplished in a number of ways.
- a portion of a H 4 gene exemplified infra can be purified and labeled to prepare a labeled probe, and the generated DNA may be screened by nucleic acid hybridization to the labeled probe (Benton and Davis, Science 1977, 196:180; Grunstein and Hogness, Proc. Natl. Acad. Sci. U.S.A. 1975, 72:3961). Those DNA fragments with substantial homology to the probe, such as an allelic variant from another individual, will hybridize. In a specific embodiment, highest stringency hybridization conditions are used to identify a homologous H 4 gene.
- the gene encodes a protein product having the isoelectric, electrophoretic, amino acid composition, partial or complete amino acid sequence, antibody binding activity, or ligand binding profile of H 4 protein as disclosed herein.
- the presence ofthe gene may be detected by assays based on the physical, chemical, immunological, or functional properties of its expressed product.
- DNA sequences which encode substantially the same amino acid sequence as a H 4 gene may be used in the practice of the present invention. These include but are not limited to allelic variants, species variants, sequence conservative variants, and functional variants.
- Amino acid substitutions may also be introduced to substitute an amino acid with a particularly preferable property.
- a Cys may be introduced a potential site for disulfide bridges with another Cys.
- H 4 derivatives and analogs ofthe invention can be produced by various methods known in the art. The manipulations which result in their production can occur at the gene or protein level.
- the cloned H 4 gene sequence can be modified by any of numerous strategies known in the art (Sambrook et al, 1989, supra). The sequence can be cleaved at appropriate sites with restriction endonuclease(s), followed by further enzymatic modification if desired, isolated, and ligated in vitro.
- the v-encoding nucleic acid sequence can be mutated in vitro or in vivo, to create and/or destroy translation, initiation, and/or termination sequences, or to create variations in coding regions and/or form new restriction endonuclease sites or destroy preexisting ones, to facilitate further in vitro modification.
- modifications can be made to introduce restriction sites and facilitate cloning the H 4 gene into an expression vector.
- Any technique for mutagenesis known in the art can be used, including but not limited to, in vitro site-directed mutagenesis (Hutchinson, C, et al, J. Biol. Chem. 253:6551, 1978; Zoller and Smith, DNA
- the identified and isolated gene can then be inserted into an appropriate cloning vector.
- vector-host systems known in the art may be used. Possible vectors include, but are not limited to, plasmids or modified viruses, but the vector system must be compatible with the host cell used. Examples of vectors include, but are not limited to, E. coli, bacteriophages such as lambda derivatives, or plasmids such as Bluescript, pBR322 derivatives or pUC plasmid derivatives, e.g., pGEX vectors, pmal-c, pFLAG, etc.
- the insertion into a cloning vector can, for example, be accomplished by ligating the DNA fragment into a cloning vector which has complementary cohesive termini.
- the ends of the DNA molecules may be enzymatically modified.
- any site desired may be produced by ligating nucleotide sequences (linkers) onto the DNA termini; these ligated linkers may comprise specific chemically synthesized oligonucleotides encoding restriction endonuclease recognition sequences.
- simple PCR or overlapping PCR may be used to insert a fragment into a cloning vector. Recombinant molecules can be introduced into host cells via transformation, transfection, infection, electroporation, etc., so that many copies of the gene sequence are generated.
- the cloned gene is contained on a shuttle vector plasmid, which provides for expansion in a cloning cell, e.g., E. coli, and facile purification for subsequent insertion into an appropriate expression cell line, if such is desired.
- a shuttle vector which is a vector that can replicate in more than one type of organism, can be prepared for replication in both E. coli and Saccharomyces cerevisiae by linking sequences from an E. coli plasmid with sequences form the yeast 2 ⁇ plasmid.
- Elements of the H 4 promoter can be identified by scanning the human genomic region upstream of the H 4 start site, e.g., by creating deletion mutants and checking for expression, or with the TRANSFAC algorithm. Sequences up to about 6 kilobases (kb) or more upstream from the H 4 start site can contain tissue-specific regulatory elements.
- H 4 promoter encompasses artificial promoters. Such promoters can be prepared by deleting nonessential intervening sequences from the upstream region ofthe H 4 promoter, or by joining upstream regulatory elements from the H 4 promoter with a heterologous minimal promoter, such as the CMV immediate early promoter.
- An H 4 promoter can be operably associated with a heterogenous coding sequence, e.g., for reporter gene (luciferase and green fluorescent proteins are examples of reporter genes) in a construct.
- This construct will result in expression ofthe heterologous coding sequence under control the H 4 promoter, e.g., a reporter gene can be expressed, under conditions that under normal conditions cause H 4 expression.
- This construct can be used in screening assays, described below, for H 4 agonists and antagonists.
- the nucleotide sequence coding for H 4 , or antigenic fragment, derivative or analog thereof, or a functionally active derivative, including a chimeric protein, thereof, can be inserted into an appropriate expression vector, /. e. , a vector which contains the necessary elements for the transcription and translation of the inserted protein-coding sequence.
- a nucleic acid encoding H 4 of the invention can be operationally associated with a promoter in an expression vector ofthe invention. Both cDNA and genomic sequences can be cloned and expressed under control of such regulatory sequences.
- Such vectors can be used to express functional or functionally inactivated H 4 polypeptides.
- the necessary transcriptional and translational signals can be provided on a recombinant expression vector, or they may be supplied by the native gene encoding H 4 and/or its flanking regions.
- Potential host-vector systems include but are not limited to mammalian cell systems transfected with expression plasmids or infected with virus (e.g., vaccinia virus, adenovirus, adeno-associated virus, herpes virus, etc.); insect cell systems infected with virus (e.g, baculovirus); microorganisms such as yeast containing yeast vectors; or bacteria transformed with bacteriophage, DNA, plasmid DNA, or cosmid DNA.
- virus e.g., vaccinia virus, adenovirus, adeno-associated virus, herpes virus, etc.
- insect cell systems infected with virus e.g, baculovirus
- microorganisms such as yeast containing yeast vectors
- bacteria transformed with bacteriophage, DNA, plasmid DNA, or cosmid DNA e.g., bacteriophage, DNA, plasmid DNA, or cosmid DNA.
- the expression elements of vectors
- H 4 protein may be controlled by any promoter/enhancer element known in the art, but these regulatory elements must be functional in the host selected for expression.
- Promoters which may be used to control H 4 gene expression include, but are not limited to, cytomegalovirus (CMV) promoter (U.S. Patent Nos. 5,385,839 and 5,168,062), the SV40 early promoter region (Benoist and Chambon, 1981, Nature 290:304-310), the promoter contained in the 3' long terminal repeat of Rous sarcoma virus (Yamamoto, et al, Cell 22:787-
- CMV cytomegalovirus
- herpes thymidine kinase promoter (Wagner et al, Proc. Natl. Acad. Sci. U.S.A. 78:1441-1445, 1981), the regulatory sequences of the metallothionein gene (Brinster et al, Nature 296:39-42, 1982); prokaryotic expression vectors such as the ⁇ -lactamase promoter (Villa- Komaroff, et al , Proc. Natl. Acad. Sci. U.S.A. 75 :3727-3731 , 1978), or the tac promoter (DeBoer, et al, Proc. Natl. Acad. Sci. U.S.A.
- Solubilized forms ofthe protein can be obtained by solubilizing inclusion bodies or reconstituting membrane components, e.g., by treatment with detergent, and if desired sonication or other mechanical processes, as described above.
- the solubilized protein can be isolated using various techniques, such as polyacrylamide gel electrophoresis (PAGE), isoelectric focusing, 2-dimensional gel electrophoresis, chromatography (e.g., ion exchange, affinity, immunoaffinity, and sizing column chromatography), centrifugation, differential solubility, immunoprecipitation, or by any other standard technique for the purification of proteins.
- PAGE polyacrylamide gel electrophoresis
- isoelectric focusing e.g., isoelectric focusing
- 2-dimensional gel electrophoresis e.g., ion exchange, affinity, immunoaffinity, and sizing column chromatography
- centrifugation e.g., centrifugation, differential solubility, immunoprecipitation, or by any other standard technique for the purification of proteins.
- Vectors A wide variety of host/expression vector combinations may be employed in expressing the DNA sequences of this invention.
- Useful expression vectors may consist of segments of chromosomal, non-chromosomal and synthetic DNA sequences.
- Suitable vectors include derivatives of SV40 and known bacterial plasmids, e.g., E.
- coli plasmids col El, pCRl, pBR322, pMal-C2, pET, pGEX (Smith et al, Gene 67:31-40, 1988), pMB9 and their derivatives, plasmids such as RP4; phage DNAS, e.g., the numerous derivatives of phage 1, e.g., NM989, and other phage DNA, e.g., M13 and filamentous single stranded phage DNA; yeast plasmids such as the 2 ⁇ plasmid or derivatives thereof; vectors useful in eukaryotic cells, such as vectors useful in insect or mammalian cells; vectors derived from combinations of plasmids and phage DNAs, such as plasmids that have been modified to employ phage DNA or other expression control sequences; and the like.
- phage DNAS e.g., the numerous derivatives of phage 1, e.g., NM9
- Viral vectors such as lentiviruses, retroviruses, herpes viruses, adenoviruses, adeno-associated viruses, vaccinia virus, baculovirus, alphavirus, and other recombinant viruses with desirable cellular tropism are also useful.
- a gene encoding a functional or mutant H 4 protein or polypeptide domain fragment thereof can be introduced in vivo, ex vivo, or in vitro using a viral vector or through direct introduction of DNA.
- Expression in targeted tissues can be effected by targeting the transgenic vector to specific cells, such as with a viral vector or a receptor ligand, or by using a tissue-specific promoter, or both. Targeted gene delivery is described in International Patent Publication WO 95/28494, published October 1995.
- Viral vectors commonly used for in vivo or ex vivo targeting and therapy procedures are DNA-based vectors and retroviral vectors. Methods for constructing and using viral vectors are known in the art (see, e.g., Miller and Rosman, BioTechniques 1992, 7:980- 990).
- the viral vectors are replication defective, that is, they are unable to replicate autonomously in the target cell.
- the genome ofthe replication defective viral vectors which are used within the scope of the present invention lack at least one region which is necessary for the replication of the virus in the infected cell. These regions can either be eliminated (in whole or in part) or be rendered non-functional by any technique known to a person skilled in the art.
- DNA viral vectors include an attenuated or defective DNA virus, such as but not limited to herpes simplex virus (HSV), papillomavirus, Epstein Barr virus (EBV), adenovirus, adeno-associated virus (AAV), and the like.
- HSV herpes simplex virus
- EBV Epstein Barr virus
- AAV adeno-associated virus
- Defective viruses which entirely or almost entirely lack viral genes, are preferred. Defective virus is not infective after introduction into a cell. Use of defective viral vectors allows for administration to cells in a specific, localized area, without concern that the vector can infect other cells. Thus, a specific tissue can be specifically targeted. Examples of particular vectors include, but are not limited to, a defective herpes virus 1 (HS V 1 ) vector (Kaplitt et al, Molec. Cell. Neurosci. 2:320-330, 1991), defective herpes virus vector lacking a glyco-protein L gene (Patent Publication RD 371005 A), or other defective herpes virus vectors (International Patent Publication No.
- HS V 1 defective herpes virus 1
- Patent Publication RD 371005 A defective herpes virus vector lacking a glyco-protein L gene
- viral vectors commercially, including but by no means limited to Avigen, Inc. (Alameda, CA; AAV vectors), Cell Genesys (Foster City, CA; retroviral, adenoviral, AAV vectors, and lentiviral vectors), Clontech (retroviral and baculoviral vectors), Genovo, Inc.
- Avigen, Inc. Almeda, CA; AAV vectors), Cell Genesys (Foster City, CA; retroviral, adenoviral, AAV vectors, and lentiviral vectors), Clontech (retroviral and baculoviral vectors), Genovo, Inc.
- an appropriate immunosuppressive treatment is employed in conjunction with the viral vector, e.g., adenovirus vector, to avoid immuno-deactivation ofthe viral vector and transfected cells.
- the viral vector e.g., adenovirus vector
- immunosuppressive cytokines such as interleukin-12 (IL-12), interferon- ⁇ (IFN- ⁇ ), or anti-CD4 antibody
- IL-12 interleukin-12
- IFN- ⁇ interferon- ⁇
- anti-CD4 antibody can be provided to block humoral or cellular immune responses to the viral vectors (see, e.g., Wilson, Nature Medicine, 1995).
- IL-12 interleukin-12
- IFN- ⁇ interferon- ⁇
- anti-CD4 antibody can be provided to block humoral or cellular immune responses to the viral vectors (see, e.g., Wilson, Nature Medicine, 1995).
- the vector can be introduced in vivo by lipofection, as naked DNA, or with other transfection facilitating agents (peptides, polymers, etc.).
- Synthetic cationic lipids can be used to prepare liposomes for in vivo transfection of a gene encoding a marker (Feigner, et al, Proc. Natl. Acad. Sci. U.S.A. 84:7413-7417, 1987; Feigner and Ringold, Science 337:387-388, 1989; see Mackey, et al, Proc. Natl. Acad. Sci. U.S.A. 85:8027-8031, 1988; Ulmer, et al, Science 259:1745-1748, 1993).
- lipid compounds and compositions for transfer of nucleic acids are described in International Patent Publications WO 95/18863 and WO 96/17823, and in U.S. Patent No. 5,459,127.
- Lipids may be chemically coupled to other molecules for the purpose of targeting (see Mackey, et al, supra).
- Targeted peptides e.g., hormones or neurotransmitters, and proteins such as antibodies, or non-peptide molecules could be coupled to liposomes chemically.
- a nucleic acid in vivo, is also useful for facilitating transfection of a nucleic acid in vivo, such as a cationic oligopeptide (e.g., International Patent Publication WO 95/21931), peptides derived from DNA binding proteins (e.g., International Patent Publication WO 96/25508), or a cationic polymer (e.g., International Patent Publication WO95/21931).
- a cationic oligopeptide e.g., International Patent Publication WO 95/21931
- peptides derived from DNA binding proteins e.g., International Patent Publication WO 96/25508
- a cationic polymer e.g., International Patent Publication WO95/21931.
- non- viral DNA vectors for gene therapy can be introduced into the desired host cells by methods known in the art, e.g., electroporation, microinjection, cell fusion, DEAE dextran, calcium phosphate precipitation, use of a gene gun (ballistic transfection; see, e.g., U.S. Pat. No. 5,204,253, U.S. Pat. No. 5,853,663, U.S. Pat. No. 5,885,795, and U.S. Pat. No. 5,702,384 and see Sanford, TIB-TECH, 6:299-302, 1988; Fynan et al, Proc. Natl. Acad. Sci.
- H ⁇ Ligands and Binding Partners The present invention further permits identification of physiological ligands and binding partners of H 4 .
- One method for evaluating and identifying H 4 binding partners is the yeast two-hybrid screen.
- the yeast two-hybrid screen is performed using an cell library with yeast that are transformed with recombinant H 4 .
- H 4 can be used as a capture or affinity purification reagent.
- labeled H 4 can be used as a probe for binding, e.g., by immunoprecipitation or Western analysis.
- Expected H 4 binding partners are G-proteins.
- binding interactions between H 4 and any of its binding partners will be strongest under conditions approximating those found in the cytoplasm, i.e., physiological conditions of ionic strength, pH and temperature. Perturbation of these conditions will tend to disrupt the stability of a binding interaction.
- Antibodies to H 4 are useful, inter alia, for diagnostics and intracellular regulation of H 4 activity, as set forth below.
- a H 4 polypeptide produced recombinantly or by chemical synthesis, and fragments or other derivatives or analogs thereof, including fusion proteins, may be used as immunogens to generate antibodies that recognize the
- H 4 polypeptide Such antibodies include but are not limited to polyclonal, monoclonal, chimeric, single chain, Fab fragments, and an Fab expression library. Such an antibody is preferfably specific for human H 4 and it may recognize either a mutant form of H 4 or wild-type H 4 , or both.
- H 4 polypeptide or derivative or analog thereof various procedures known in the art may be used for the production of polyclonal antibodies to H 4 polypeptide or derivative or analog thereof.
- various host animals can be immunized by injection with the H 4 polypeptide, or a derivative (e.g., fragment or fusion protein) thereof, including but not limited to rabbits, mice, rats, sheep, goats, etc.
- the H 4 polypeptide or fragment thereof can be conjugated to an immunogenic carrier, e.g., bovine serum albumin (BSA) or keyhole limpet hemocyanin (KLH).
- BSA bovine serum albumin
- KLH keyhole limpet hemocyanin
- adjuvants may be used to increase the immunological response, depending on the host species, including but not limited to Freund's (complete and incomplete), mineral gels such as aluminum hydroxide, surface active substances such as lysolecithin, pluronic polyols, polyanions, peptides, oil emulsions, keyhole limpet hemocyanins, dinitrophenol, and potentially useful human adjuvants such as BCG (bacille Calmette-Guerin) and Corynebacterium parvum.
- BCG Bacille Calmette-Guerin
- Corynebacterium parvum bacille Calmette-Guerin
- any technique that provides for the production of antibody molecules by continuous cell lines in culture may be used. These include but are not limited to the hybridoma technique originally developed by Kohler and Milstein (Nature 1975,
- monoclonal antibodies can be produced in germ-free animals (International Patent Publication No. WO 89/12690).
- chimeric antibodies Infact, according to the invention, techniques developed for the production of "chimeric antibodies" (Morrison et al. , J. Bacteriol. 1984, 159:870; Neuberger et al, Nature 1984, 312:604-608; Takeda et al, Nature 1985, 314:452-454) by splicing the genes from a mouse antibody molecule specific for an H 4 polypeptide together with genes from a human antibody molecule of appropriate biological activity can be used; such antibodies are within the scope of this invention.
- Such human or humanized chimeric antibodies are preferred for use in therapy of human diseases or disorders (described infra), since the human or humanized antibodies are much less likely than xenogenic antibodies to induce an immune response, in particular an allergic response, themselves.
- Antibody fragments which contain the idiotype ofthe antibody molecule can be generated by known techniques.
- such fragments include but are not limited to: the F(ab')2 fragment which can be produced by pepsin digestion ofthe antibody molecule; the Fab' fragments which can be generated by reducing the disulfide bridges ofthe F(ab')2 fragment, and the Fab fragments which can be generated by treating the antibody molecule with papain and a reducing agent.
- screening for or testing with the desired antibody can be accomplished by techniques known in the art, e.g., radio immunoassay, ELISA (enzyme-linked immunosorbant assay), "sandwich” immunoassays, immunoradiometric assays, gel diffusion precipitin reactions, immunodiffusion assays, in situ immunoassays (using colloidal gold, enzyme or radioisotope labels, for example), Western blots, precipitation reactions, agglutination assays (e.g., gel agglutination assays, hemagglutination assays), complement fixation assays, immunofluorescence assays, protein A assays, and immunoelectrophoresis assays, etc.
- radio immunoassay e.g., radio immunoassay, ELISA (enzyme-linked immunosorbant assay), "sandwich” immunoassays, immunoradiometric assays, gel diffusion precipitin reactions, immunodiffusion assays, in
- antibody binding is detected by detecting a label on the primary antibody.
- the primary antibody is detected by detecting binding of a secondary antibody or reagent to the primary antibody.
- the secondary antibody is labeled.
- Many means are known in the art for detecting binding in an immunoassay and are within the scope of the present invention. For example, to select antibodies which recognize a specific epitope of an H 4 polypeptide, one may assay generated hybridomas for a product which binds to an H 4 polypeptide fragment containing such epitope. For selection of an antibody specific to an H 4 polypeptide from a particular species of animal, one can select on the basis of positive binding with H 4 polypeptide expressed by or isolated from cells of that species of animal.
- the foregoing antibodies can be used in methods known in the art relating to the localization and activity ofthe H 4 polypeptide, e.g., for Western blotting, imaging H 4 polypeptide in situ, measuring levels thereof in appropriate physiological samples, etc. using any of the detection techniques mentioned above or known in the art.
- Such antibodies can also be used in assays for ligand binding, e.g., as described in U.S. Patent No. 5,679,582.
- Antibody binding generally occurs most readily under physiological conditions, e.g., pH of between about 7 and 8, and physiological ionic strength. The presence of a carrier protein in the buffer solutions stabilizes the assays. While there is some tolerance of perturbation of optimal conditions, e.g., increasing or decreasing ionic strength, temperature, or pH, or adding detergents or chaotropic salts, such perturbations will decrease binding stability.
- antibodies that act as ligands and agonize or antagonize the activity of H 4 polypeptide can be generated.
- intracellular single chain Fv antibodies can be used to regulate cAMP formation (Marasco et al., Proc. Natl. Acad. Sci. U.S.A. 1993, 90:7884-7893; Chen., Mol. Med. Today 1997, 3:160-167; Spitz et al., Anticancer Res.
- nucleotide sequences encoding H 4 and the H 4 receptor structure which can be modeled from the amino acid sequence based on homology to other GPCR proteins, are useful targets to identify drugs that are effective in treating disorders associated with histamine-regulated processes.
- Drug targets include without limitation (i) isolated nucleic acids derived from the gene encoding H 4 (e. g. , antisense or ribozyme molecules) and (ii)small molecule compounds that recognize and bind the receptor.
- identification and isolation of H 4 provides for development of screening assays, particularly for high throughput screening of molecules that up- or down- regulate the activity of H 4 .
- the present invention contemplates methods for identifying specific histamine receptor ligands that interact with H 4 receptors, using various screening assays known in the art.
- Any screening technique known in the art can be used to screen for H 4 agonists or antagonists.
- the present invention contemplates screens for small molecule ligands or ligand analogs and mimics, as well as screens for natural ligands that bind to and agonize or antagonize H 4 activity in vivo.
- natural products libraries can be screened using assays ofthe invention for molecules that agonize or antagonize H 4 expression or activity.
- synthetic libraries (Needels et al, Proc. Natl. Acad. Sci. USA 1993, 90:10700-4; Ohlmeyer et al, Proc. Natl. Acad. Sci. USA 1993, 90:10922-10926; Lam etal, PCT Publication No. WO 92/00252; Kocis etal, PCT Publication No. WO 9428028) and the like can be used to screen for ligands that regulate H 4 activity. Test compounds are screened from large libraries of synthetic or natural compounds. Numerous means are currently used for random and directed synthesis of saccharide, peptide, and nucleic acid based compounds. Synthetic compound libraries are commercially available from Maybridge Chemical Co.
- test wells containing an aliquot of a lipid bilayer membranes that contain the histamine H 4 receptor are incubated with an known concentration of a radiolabeled ligand for the receptor.
- the lipid bilayer may be prepared by any known protocol that separates the membrane containing receptor component from the cytoplasmic components.
- Each well also is incubated with a different concentration of a unlabeled test compound.
- Cell membranes are then separated from the incubation mixture by any method known in the art including, but not limited to, centrifugation and vacuum filtration on a cell harvester.
- the radioactivity of each well is then determined using any device that can detect radioactivity, such as a scintillation counter. As increasing concentrations of the test compound compete for the receptor binding site, the radioactivity detected decreases. The data then can be converted using the Cheng-Prusoff equation (Biochem Pharmacol. 1973, 22:3099-3108) to determine the affinity (K-) ofthe compound for the receptor.
- In vivo screening methods Intact cells or whole animals expressing a gene encoding H 4 can be used in screening methods to identify candidate drugs.
- a permanent cell line is established.
- cells including without limitation mammalian, insect, yeast, or bacterial cells
- Identification of candidate compounds can be achieved using any suitable assay, including without limitation
- H 4 knockout mammals can be prepared for evaluating the molecular pathology of this defect in greater detail than is possible with human subjects. Such animals also provide excellent models for screening drug candidates.
- a "knockout mammal” is an mammal (e.g., mouse, rabbit) that contains within its genome a specific gene that has been inactivated by the method of gene targeting (see, e.g., U.S. Patent Nos. 5,777,195 and 5,616,491).
- a knockout mammal includes both a heterozygote knockout (i.e., one defective allele and one wild-type allele) and a homozygous mutant (i.e., two defective alleles; a heterologous construct for expression of an H 4 , such as a human H 4 , could be inserted to permit the knockout mammal to live if lack of H 4 expression was lethal).
- Preparation of a knockout mammal requires first introducing a nucleic acid construct that will be used to suppress expression of a particular gene into an undifferentiated cell type termed an embryonic stem cell. This cell is then injected into a mammalian embryo. A mammalian embryo with an integrated cell is then implanted into a foster mother for the duration of gestation.
- Zhou, et al. (Genes and Development 1995, 9:2623-34) describes PPCA knock-out mice.
- knockout refers to partial or complete suppression ofthe expression of at least a portion of a protein encoded by an endogenous DNA sequence in a cell.
- knockout construct refers to a nucleic acid sequence that is designed to decrease or suppress expression of a protein encoded by endogenous DNA sequences in a cell.
- the nucleic acid sequence used as the knockout construct is typically comprised of (1) DNA from some portion ofthe gene (exon sequence, intron sequence, and/or promoter sequence) to be suppressed and (2) a marker sequence used to detect the presence of the knockout construct in the cell.
- the knockout construct is inserted into a cell, and integrates with the genomic DNA ofthe cell in such a position so as to prevent or interrupt transcription of the native DNA sequence.
- Such insertion usually occurs by homologous recombination (i.e., regions ofthe knockout construct that are homologous to endogenous DNA sequences hybridize to each other when the knockout construct is inserted into the cell and recombine so that the knockout construct is inco ⁇ orated into the corresponding position ofthe endogenous DNA).
- the knockout construct nucleic acid sequence may comprise (1) a full or partial sequence of one or more exons and/or introns ofthe gene to be suppressed, (2) a full or partial promoter sequence ofthe gene to be suppressed, or (3) combinations thereof.
- the knockout construct is inserted into an embryonic stem cell (ES cell) and is integrated into the ES cell genomic DNA, usually by the process of homologous recombination. This ES cell is then injected into, and integrates with, the developing embryo.
- ES cell embryonic stem cell
- disruption ofthe gene and “gene disruption” refer to insertion of a nucleic acid sequence into one region ofthe native DNA sequence (usually one or more exons) and/or the promoter region of a gene so as to decrease or prevent expression of that gene in the cell as compared to the wild-type or naturally occurring sequence of the gene.
- a nucleic acid construct can be prepared containing a DNA sequence encoding an antibiotic resistance gene which is inserted into the DNA sequence that is complementary to the DNA sequence (promoter and/or coding region) to be disrupted. When this nucleic acid construct is then transfected into a cell, the construct will integrate into the genomic DNA. Thus, many progeny ofthe cell will no longer express the gene at least in some cells, or will express it at a decreased level, as the DNA is now disrupted by the antibiotic resistance gene.
- the DNA will be at least about 1 kb in length and preferably 3-4 kb in length, thereby providing sufficient complementary sequence for recombination when the knockout construct is introduced into the genomic DNA ofthe ES cell (discussed below).
- a mammal in which two or more genes have been knocked out. Such mammals can be generated by repeating the procedures set forth herein for generating each knockout construct, or by breeding to mammals, each with a single gene knocked out, to each other, and screening for those with the double knockout genotype.
- Regulated knockout animals can be prepared using various systems, such as the tet-repressor system (see U.S. Patent No.5,654,168) or the Cre-Lox system (see U.S. Patent Nos. 4,959,317 and 5,801,030).
- transgenic animals are created in which (i) a human H 4 is stably inserted into the genome ofthe transgenic animal; and/or (ii) the endogenous H 4 genes are inactivated and replaced withhuman H 4 genes. See, e.g., Coffman, Semin. Nephrol.
- H 4 Activation Assay Any cell assay system that allows for assessment of functional activity of H 4 agonists and antagonists is defined by the present invention.
- the assay can be used to identify compounds that selectively interact with H 4 , which can be evaluated by assessing the effects of H 4 transformed cells contacted with a test compound, which modulates cAMP accumulation.
- the assay system can thus be used to identify compounds that selectively produce a functional effect through histamine H 4 receptors.
- Compounds that increase cAMP formation and accumulation may be useful as novel therapeutics in the prevention of transplanted organ rej ection, asthma, allergies, and autoimmune pathologies, such as, but not limited to, multiple sclerosis, type I diabetes, and rheumatoid arthritis. Additionally, CNS functions such as cognitive and memory defects may be treated.
- each experiment is performed in triplicate at multiple different dilutions of test compound.
- An agonist and/or antagonist screen involves detecting cAMP accumulation by the host cell when contacted with H 4 ligand. If cAMP accumulation is increased, the test compound is a candidate antagonist of H 4 receptors. If cAMP accumulation is decreased, the test compound is a candidate agonist of H 4 receptors. If there is no change in cAMP formation, the test compound is not an effective H 4 ligand.
- the invention provides immunoassays for detecting cAMP.
- immunoassays use either a labeled antibody or a labeled antigenic component (e.g., that competes with the antigen in the sample for binding to the antibody).
- Suitable labels include without limitation enzyme-based, fluorescent, chemiluminescent, radioactive, or dye molecules.
- Assays that amplify the signals from the probe are also known, such as, for example, those that utilize biotin and avidin, and enzyme-labelled immunoassays, such as ELISA assays.
- labeled antigenic component may be quantified by scintillation techniques.
- the second messenger preferably cAMP, will be separated on a high performance liquid chromatograph and quantified by a UV detector.
- the assay system described here also may be used in a high-throughput primary screen for agonists and antagonists, or it may be used as a secondary functional screen for candidate compounds identified by a different primary screen, e.g., a binding assay screen that identifies compounds that interact with the receptor.
- High-Throughput Screen Agents according to the invention may be identified by screening in high- throughput assays, including without limitation cell-based or cell-free assays. It will be appreciated by those skilled in the art that different types of assays can be used to detect different types of agents. Several methods of automated assays have been developed in recent years so as to permit screening of tens of thousands of compounds in a short period of time. Such high- throughput screening methods are particularly preferred. The use of high-throughput screening assays to test for agents is greatly facilitated by the availability of large amounts of purified polypeptides, as provided by the invention.
- Hetamine refers to a neurotransmitter that is produced and released from neurons. Histamine is formed from the amino acid histidine by histidine decarboxylase.
- histamine is an imidazolethylamine.
- histamine is comprised of an imidazole moiety and an amino group connected by an alkyl chain.
- the pharmacologically active form of histamine is proposed to be the monocationic tautomer, where one ofthe nitrogen present in the imidazole ring is positively charged.
- different forms of histamine may interact with histamine receptors to produce a functional effect. Histamine is produced intracellularly and stored until released in response to a physiological stimulus.
- Hyistamine analogs refers to compounds that comprise the imidazole, alkyl chain, and a nitrogen moiety (e.g., amine (unsubstituted or substituted), piperidine, pyridine) of histamine, but may be modified at other positions. These modifications may be performed to alter affinity and/or selectivity ofthe compound for the histamine receptors.
- Heistamine compounds refers to compounds that may bind to the histamine receptors.
- Histamine analogs and compounds can be classified as agonists or antagonists. As discussed previously, agonists are ligands that bind to the receptor and produce a functional effect similar to that produced by the endogenous ligand (i.e., histamine) for the receptor, whereas antagonists are ligands that bind to the receptor and block a functional effect produced by an agonist for the receptor or the endogenous ligand ofthe receptor. Histamine analogs and compounds are further described in Goodman and Gilman's The Pharmacological Basis of Therapeutics, Ninth Edition, McGraw-Hill, 1996.
- Agonists that may be contemplated by this invention include, but are not limited to, R-( ⁇ )-methylhistamine, imetit, and immepip. Antagonists, burimamide, impromidine, dimaprit, and thioperamide clobenpropit and iodophenpropit impentamine, GT2016 and iodoproxyfan. Other compounds include derivatives, metabolites, and precursors.
- genetic variants of H 4 can be detected to diagnose an H 4 associated disease, such as treatment for transplanted organ rejection, asthma, allergies, and autoimmune pathologies, such as, but not limited to, multiple sclerosis, type I diabetes, and rheumatoid arthritis. Additionally, CNS functions such as cognitive and memory defects may be treated. The various methods for detecting such variants are described herein. Where such variants impact H 4 function, either as a result of a mutated amino acid sequence or because the mutation results in expression of a truncated protein, or no expression at all, they are expected to result in disregulation ofthe allergic response, the immune response, cognition and memory.
- the DNA may be obtained from any cell source.
- DNA is extracted from the cell source or body fluid using any ofthe numerous methods that are standard in the art. It will be understood that the particular method used to extract DNA will depend on the nature ofthe source. Generally, the minimum amount of DNA to be extracted for use in the present invention is about 25 pg (corresponding to about 5 cell equivalents of a genome size of 4 x
- RNA is isolated from biopsy tissue using standard methods well known to those of ordinary skill in the art such as guanidium thiocyanate-phenol-chloroform extraction (Chomocyznski et al, Anal. Biochem., 162: 156, 1987).
- the isolated RNA is then subjected to coupled reverse transcription and amplification by polymerase chain reaction (RT-PCR), using specific oligonucleotide primers that are specific for a selected site.
- RT-PCR polymerase chain reaction
- Conditions for primer annealing are chosen to ensure specific reverse transcription and amplification; thus, the appearance of an amplification product is diagnostic ofthe presence of a particular genetic variation.
- RNA is reverse-transcribed and amplified, after which the amplified sequences are identified by, e.g., direct sequencing.
- cDNA obtained from the RNA can be cloned and sequenced to identify a mutation.
- biopsy tissue is obtained from a subject.
- Antibodies that are capable of specifically binding to H 4 are then contacted with samples of the tissue to determine the presence or absence of a H 4 polypeptide specified by the antibody.
- the antibodies may be polyclonal or monoclonal, preferably monoclonal. Measurement of specific antibody binding to cells may be accomplished by any known method, e.g., quantitative flow cytometry, enzyme-linked or fluorescence-linked immunoassay, Western analysis, etc. Therapeutic Uses
- stimulation of H 4 receptor activity may be used as a treatment option in patients with histamine-related disease states.
- Stimulation of H 4 receptor activity may be by methods, such as, but not limited to, (i) providing polypeptides that stimulate receptor activity and (ii) providing compounds that stimulate receptor activity.
- vectors comprising a sequence encoding a protein, including, but not limited to, full-length H 4 , are provided to treat or prevent a disease or disorder associated with the function of H 4 in peripheral blood leukocytes.
- the therapeutic vector encodes a sequence that produces the protein ofthe invention.
- the therapeutic vector comprises a nucleic acid that expresses a protein of the invention in a suitable host.
- a vector has a promoter operationally linked to the coding sequence for the protein.
- the promoter can be inducible or constitutive and, optionally, tissue-specific.
- a nucleic acid molecule is used in which the protein coding sequences and any other desired sequences are flanked by regions that promote homologous recombination at a desired site in the genome, thus providing for intrachromosomal expression of the protein (Koller and Smithies, Proc. Natl. Acad. Sci.
- Delivery ofthe vector into a patient may be either direct, in which case the patient is directly exposed to the vector or a delivery complex, or indirect, in which case, cells are first transformed with the vector in vitro then transplanted into the patient.
- direct in which case the patient is directly exposed to the vector or a delivery complex
- indirect in which case, cells are first transformed with the vector in vitro then transplanted into the patient.
- the vector is directly provided in vivo, where it enters the cells ofthe organism and mediates expression ofthe protein.
- This can be accomplished by any of numerous methods known in the art, by constructing it as part of an appropriate expression vector and administering it so that it becomes intracellular, e.g., by infection using a defective or attenuated retroviral or other viral vector (see, U.S. Patent No.
- a nucleic acid ligand complex can be formed in which the ligand comprises a fusogenic viral peptide to disrupt endosomes, allowing the nucleic acid to avoid lysosomal degradation.
- the nucleic acid can be targeted in vivo for cell specific uptake and expression, by targeting a specific receptor (see, e.g., PCT Publication Nos. WO 92/06180, WO 92/22635, WO 92/20316 and WO 93/14188).
- the nucleic acid can be introduced intracellularly and inco ⁇ orated within host cell DNA for expression by homologous recombination (Koller and Smithies, Proc. Natl. Acad. Sci.
- the form and amount of therapeutic nucleic acid envisioned for use depends on the type of disease and the severity ofthe desired effect, patient state, etc., and can be determined by one skilled in the art.
- Exogenous compounds may interact with extracellular and/or intracellular messenger systems, such as, but not limited to, adenosine triphosphate, nitric oxide, and guanosine triphosphate; to regulate protein synthesis.
- exogenous compounds that stimulate or inhibit H 4 protein synthesis may be used in the prevention and/or treatment for transplanted organ rejection, asthma, allergies, and autoimmune pathologies, such as, but not limited to, multiple sclerosis, type I diabetes, and rheumatoid arthritis.
- CNS functions such as cognitive and memory defects may be treated.
- the present invention provides antisense nucleic acids (including ribozymes), which may be used to inhibit expression of H 4 ofthe invention.
- the antisense nucleic acid upon hybridizing under cytoplasmic conditions with complementary bases in an RNA or DNA molecule, inhibits the role ofthe RNA or DNA. Additionally, hybridization ofthe antisense nucleic acid to the DNA or RNA may inhibit transcription ofthe DNA into RNA and/or translation of the RNA into the protein. If the RNA is a messenger RNA transcript, the antisense nucleic acid is a countertranscript or mRNA-interfering complementary nucleic acid.
- Antisense nucleic acid molecules can be encoded by a recombinant gene for expression in a cell (e.g., U.S.
- Patent No. 5,814,500 can be prepared synthetically (e.g., U.S. Patent No. 5,780,607).
- antibody molecules can also be administered, for example, by expressing nucleotide sequences encoding single-chain antibodies within the target cell population by utilizing, for example, techniques such as those described in Marasco et al. (Proc. Natl. Acad Sci. USA, 1993, 90:7889-7893).
- Therapeutically suggested compounds may be provided to the patient in formulations that are known in the art and may include any pharmaceutically acceptable additives, such as excipents, lubricants, diluents, flavorants, colorants, and disintegrants.
- the formulations may be produced in useful dosage units such as tablet, caplet, capsule, liquid, or injection.
- the form and amount of therapeutic compound envisioned for use depends on the type of disease and the severity ofthe desired effect, patient state, etc., and can be determined by one skilled in the art.
- a GPCR sequence profile was generated from a sequence alignment of members of this subfamily based on hidden Markov Models (Eddy Bioinformatics, 1998 14:755-763; Durbin et al. A tutorial introduction to hidden Markov models and other probabilistic modeling approaches in computational sequence analysis, Cambridge University Press, 1998) to look for novel members in the human genome database.
- GPCR proteins ofthe biogenic amine subfamily were retrieved from S wiss-Prot database and a sequence alignment was generated by a multiple sequence alignment tool, named CLUSTALW (Thompson et al. Nucleic Acids Res, 1994, 22:4673-80).
- CLUSTALW Thimpson et al. Nucleic Acids Res, 1994, 22:4673-80.
- LLFLLTLPPW ALYYLVGGSE DWPFGSALCK LVTALDVVNM YASILLLTAI SIDRYLAIVH PLRYRRRRTS PRRAKVVILL VWVLALLLSL PPLLFSWVKT VEEGNGTLNV NVTVCLIDFP EESTAS VSTW LRS YVLLSTL VGFLLPLLVI LVC YTRJLRT LRKAAKTLLV VVVVFVLCWL PYFIVLLLDT LCLSIIMSST CELERVLPTA LLVTLWLAYV NSCLNPIIY; SEQ ID NO: 3) was developed from the biogenic amine subfamily members. The consensus sequence contained the unique 7-transmembrane sequence structure of biogenic amine GPCRs.
- Quantitative RT-PCR was performed on an ABI 7700 "Taqman" sequence detection system to determine the tissue distribution ofthe H 4 receptor.
- Primers spanning the exon 1-2 boundary (Forward primer: 5'-taacttggccattgacttctt-3' (SEQ ID NO:4); Reverse primer:
- 5'-attcgaacagcatgtgagggat-3' (SEQ ID NO: 5) and a Probe: 5'-(6-carboxyfluorescein)- tacaaaggaatggagatcacacccaca- (6-carboxy-N,N,N'N'-tetramethylrhodamine)-3' (SEQ ID NO: 6)) were used to determine H 4 expression levels in a mRNA prepared from a series of human tissues, purchased from Clontech (Palo Alto). A 2 step reaction procedure was performed as per manufacturers directions. Briefly, 2mg RNA was reverse transcribed using random hexamers
- H 4 receptor protein coding sequences were amplified using Forward oligo:
- the yeast expression plasmid, pMP327 was introduced into MPN578i5 cells (MATa ura3 his3 tipl leu2 lys2 ade2 farl::LNS2 fusl ::FUSl-HIS3 sst2::SST2-G418R ste2::LEU2, gpal::GPAli5) (Hadcock, J.R. and Pausch, M.H. submitted) using LiOAc and selected for ura prototrophy.
- MPN578i5 cells express a chimeric
- the G alpha protein coupled to the mating signal transduction pathway is expressed from the GPA1 locus and is composed of Gpal sequences in which the 5'-C-terminal amino acids have been replaced with those of Gai3.
- a multicopy FUSl-LacZ reporter gene plasmid, pMP283 (Hadcock, J.R. and Pausch, M.H. submitted), was subsequently introduced into H 4 R-containing MPY578i5 cells and selected on media lacking tip and ura. The resulting yeast strain, MPY733, was used for further analysis.
- the ORF was modified by PCR for mammalian expression of H 4 .
- a 5' Hindlll restriciton enzymes site and a Kozak consensus sequence were added using the primer 5'- aagcttccaccatgccagatactaatagcacaatcaatttatc-3' (SEQ ID NO:9), and a 3' Xbal site added 5'- tctagattaagaagatactgaccgactgtgttg-3 ' (SEQ ID NO : 10) .
- the sequence was confirmed and ligated into the Hindlll and Xba I sites of the mammalian expression vector pCDNA3.1+ zeo (Invitrogen, Carlsbad CA).
- HEK 293 cells (approximately 10 7 cells) were transfected with the pCDN A3.1 +zeo/H 4 using standard lipofectamine plus reagent (Life Technologies) .
- Cells were maintained in DMEM containing 10% fetal calf serum and penicillin ( 100units/ml)/streptomycin (lOOmg/ml).
- 48 hours post transfection cells were selected using 500mg/ml zeocin.
- Zeocin resistant clones were assayed using the cAMP assay and by RT-PCR.
- RNA was extracted from approximately 10 6 cells using One step PCR kit (Life technologies).
- the primers used in the extraction were 5'-ggaaggatgaaggtagtgaatg-3' (SEQ ID NO: 11) and 5'-cagaatctgattgggaggaagg-3' (SEQ ID NO: 12).
- HEK cells stably expressing the H 4 receptor were assessed functionally in cAMP assays using the cAMP scintillation proximity assay (SPA) (Amersham Pharmacia Biotech, Pistcataway, NJ). Briefly, 40 000 cells were plated into wells of a 96 well plate. 24 hours later the media was removed and replaced with lOO ⁇ l Krebs bicarbonate buffer and the cells were incubated at 37°C for 15 minutes. Following this, the cells were incubated in Krebs buffer containing 0.5mM isobutylmethylxanthine, a phosphodiesterase inhibitor, to prevent cAMP breakdown. The effect of H 4 on the forskolin induced formation was determined by incubating the cells in the presence of forskolin (lO ⁇ M) and agonist for 12 minutes. cAMP levels were determined using the cAMP SPA kit according to the manufacturers directions.
- SPA cAMP scintillation proximity assay
- EXAMPLE N H ⁇ EXPRESSION IN INDUCED CD4 AND CD8 CLONES
- RNA for the standard curve was polyA+ human leukocyte RNA from Clontech.
- H 4 expression was normalized with GAPDH.
- HMM Hidden Markov Model
- the predicted protein sequence contains residues that are conserved across the biogenic amine receptors. As in other biogenic amine receptors, the present protein contains conserved aspartate residues within transmembrane (TM) domains 2 and 3 (at positions 61 and 94). A DY (D94 and Y95) motif in TM3 is found only in histamine and muscarinic receptors.
- a DRY sequence motif that exists at the cytoplasmic interface of TM3 of all GPCRs also can be found in the H 4 sequence (amino acids 111- 113). Also present are two conserved cysteine residues in the first and second extracellular loops (position 88 and 164), which are predicted to form a disulfide bond; two conserved tryptophan residues in TM4 and TM6 (positions 140 and 316); and conserved proline residues in TM5, TM6, and TM7 (positions 186, 318, and 355, respectively). Based on these results, H 4 cDNA appears to encode a biogenic amine-like receptor.
- the predicted open reading frame is 1173 base pairs long and encodes a protein of 390 amino acids. Sequence comparison using BLASTP, under standard conditions, analysis reveals that the novel protein is most similar to the human H 3 receptor (44%> identical and 51 %> similar). Based on sequence homology it is proposed that the receptor belongs to the histamine receptor family, therefore we have termed it the H 4 receptor.
- Receptor distribution studies indicate that the present receptor is highly expressed in peripheral blood leukocytes. Trace amounts ofthe H 4 receptor are expressed in heart, lung and placenta. It is proposed that these trace amounts of expression represent blood cell mRNA present in the samples. Functional studies further confirmed the categorization ofthe protein as a novel histamine receptor. Histamine and the H 3 selective agonist R- ⁇ -methylhistamine stimulated cAMP accumulation, suggesting these compounds are agonists at the H 4 receptor (See Figure 2A).
- R- ⁇ -methylhistamine exhibited both lower potency and efficacy than histamine at the H 4 receptor despite being more potent at the related cloned H 3 receptor and in several tissue based assays for the H 3 receptor (Vollinga, et al, J. Med. Chem. 1995, 38:266-271; Ha ⁇ er, etal, Br. J. Pharmacol. 1999, 128:751-759).
- the selective H 3 receptor antagonist clobenpropit was a partial agonist at the H 4 receptor (See Figure 2A).
- H 3 antagonist thioperamide almost fully inhibited the stimulatory response produced by histamine (See Figure 2B). Comparatively, clobenpropit partially blocked histamine-induced stimulation of cAMP formation, further suggesting that clobenpropit is a partial agonist at the H 4 receptor.
- the interaction ofthe H 4 receptor to a GPAI/ G ⁇ i3 chimeric G-protein alpha subunit also predicts its coupling specificity in mammalian cells.
- H 4 receptor in HEK 293tsa cells confirms the coupling ofthe H 4 receptor to the inhibition of cAMP formation (See Figure 2C).
- Forskolin stimulated 8-fold greater cAMP formation compared to basal levels.
- H 4 expression normalized with GAPDH, was detectable in all samples at all time points ( Figure 5).
- Anti-CD3 stimulation produced significant induction of H 4 expression in all 6 samples, most peaking at the 8 hour time point.
- the highest level of induction was found in the 8 hour CD4+ and CD8+ samples from the same donor (designated 'U'), peaking at greater than 100 copies of H 4 RNA per copy of GAPDH. Combined, these studies indicate that the pharmacological profile of the H 4 receptor is not similar to any known histamine H 3 receptor.
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WO2003057919A2 (en) * | 2001-12-21 | 2003-07-17 | Ortho-Mcneil Pharmaceutical, Inc. | The use of histamine h4 receptor antagonist for the treatment of inflammatory response |
WO2004021999A2 (en) * | 2002-09-06 | 2004-03-18 | Janssen Pharmaceutica, N.V. | Use of histamine h4 receptor modulators for the treatment of allergy and asthma |
US7115395B2 (en) | 2000-05-31 | 2006-10-03 | Ortho-Mcneil Pharmaceutical, Inc. | DNAs encoding guinea pig histamine receptors of the H4 subtype |
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WO2000022131A2 (en) * | 1998-10-13 | 2000-04-20 | Arena Pharmaceuticals, Inc. | Non-endogenous, constitutively activated human g protein-coupled receptors |
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Non-Patent Citations (3)
Title |
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LOVENBERG T W ET AL: "Cloning and functional expression of the human histamine H3 receptor" MOLECULAR PHARMACOLOGY, BALTIMORE, MD, US, vol. 55, 1999, pages 1101-1107, XP002942531 ISSN: 0026-895X * |
NAKAMURA ET AL: "Molecular cloning and characterization of a new human histamine receptor, HH4R." BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 279, December 2000 (2000-12), pages 615-620, XP002185552 * |
ODA ET AL: "Molecular cloning and characterization of a novel type of histamine receptor preferentially expressed in leukocytes" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOGICAL CHEMISTS, BALTIMORE, MD, US, vol. 275, no. 47, 5 September 2000 (2000-09-05), pages 36781-36786, XP002942018 ISSN: 0021-9258 * |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7115395B2 (en) | 2000-05-31 | 2006-10-03 | Ortho-Mcneil Pharmaceutical, Inc. | DNAs encoding guinea pig histamine receptors of the H4 subtype |
US7183078B2 (en) | 2000-05-31 | 2007-02-27 | Ortho-Mcneil Pharmaceutical, Inc. | DNAs encoding mammalian histamine receptor of the H4 subtype and encoded protein |
US7202057B2 (en) | 2000-05-31 | 2007-04-10 | Ortho-Mcneil Pharmaceutical, Inc. | DNAs encoding rat histamine receptors of the H4 subtype |
WO2003057919A2 (en) * | 2001-12-21 | 2003-07-17 | Ortho-Mcneil Pharmaceutical, Inc. | The use of histamine h4 receptor antagonist for the treatment of inflammatory response |
WO2003057919A3 (en) * | 2001-12-21 | 2003-10-30 | Ortho Mcneil Pharm Inc | The use of histamine h4 receptor antagonist for the treatment of inflammatory response |
WO2004021999A2 (en) * | 2002-09-06 | 2004-03-18 | Janssen Pharmaceutica, N.V. | Use of histamine h4 receptor modulators for the treatment of allergy and asthma |
WO2004021999A3 (en) * | 2002-09-06 | 2004-10-07 | Janssen Pharmaceutica Nv | Use of histamine h4 receptor modulators for the treatment of allergy and asthma |
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