WO2001077350A3 - Compositions and methods for inhibiting gene expression - Google Patents

Compositions and methods for inhibiting gene expression Download PDF

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Publication number
WO2001077350A3
WO2001077350A3 PCT/US2001/011436 US0111436W WO0177350A3 WO 2001077350 A3 WO2001077350 A3 WO 2001077350A3 US 0111436 W US0111436 W US 0111436W WO 0177350 A3 WO0177350 A3 WO 0177350A3
Authority
WO
WIPO (PCT)
Prior art keywords
methods
present
eukaryotic cell
endogenous gene
compositions
Prior art date
Application number
PCT/US2001/011436
Other languages
French (fr)
Other versions
WO2001077350A2 (en
Inventor
Kenneth E Palmer
Gregory P Pogue
Original Assignee
Large Scale Biology Corp
Kenneth E Palmer
Gregory P Pogue
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Large Scale Biology Corp, Kenneth E Palmer, Gregory P Pogue filed Critical Large Scale Biology Corp
Priority to AU2001253255A priority Critical patent/AU2001253255A1/en
Publication of WO2001077350A2 publication Critical patent/WO2001077350A2/en
Publication of WO2001077350A3 publication Critical patent/WO2001077350A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts

Abstract

The present invention provides an eukaryotic recombinant vector suited for bi-directional transcription of a transgene to yield both sense and antisense RNA transcripts of the transgene in an eukaryotic cell. The invention vectors are particularly suited for mediating gene silencing in a variety of biological systems. The present invention also provides host cells and transgenic plants comprising the invention vectors. Further provided by the invention are methods of inhibiting expression of an endogenous gene present in an eukaryotic cell. Also included is a method of identifying a biological function(s) of an endogenous gene of interest in an eukaryotic cell by selectively inhibiting the expression of the endogenous gene.
PCT/US2001/011436 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression WO2001077350A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU2001253255A AU2001253255A1 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US54557400A 2000-04-07 2000-04-07
US09/545,574 2000-04-07

Publications (2)

Publication Number Publication Date
WO2001077350A2 WO2001077350A2 (en) 2001-10-18
WO2001077350A3 true WO2001077350A3 (en) 2002-08-08

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/011436 WO2001077350A2 (en) 2000-04-07 2001-04-04 Compositions and methods for inhibiting gene expression

Country Status (2)

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AU (1) AU2001253255A1 (en)
WO (1) WO2001077350A2 (en)

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US6586661B1 (en) 1997-06-12 2003-07-01 North Carolina State University Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid
AU2001297906B2 (en) * 2000-10-31 2007-07-19 Commonwealth Scientific And Industrial Research Organisation Method and means for producing barley yellow dwarf virus resistant cereal plants
WO2004033620A2 (en) 2001-11-02 2004-04-22 Insert Therapeutics, Inc. Methods and compositions for therapeutic use of rna interference
US20040009946A1 (en) 2002-05-23 2004-01-15 Ceptyr, Inc. Modulation of PTP1B expression and signal transduction by RNA interference
US7148342B2 (en) 2002-07-24 2006-12-12 The Trustees Of The University Of Pennyslvania Compositions and methods for sirna inhibition of angiogenesis
AU2002951347A0 (en) * 2002-09-09 2002-09-26 Benitec Australia Ltd Genetic silencing
CA2521649C (en) 2003-04-07 2013-05-28 Glycogenesys, Inc. Composition and uses of galectin antagonists
WO2005097207A2 (en) 2004-03-26 2005-10-20 Curis, Inc. Rna interference modulators of hedgehog signaling and uses thereof
EP2289534B1 (en) 2004-04-05 2017-08-30 The Regents of The University of California NKG2D antibodies for use in the treatment of rheumatoid arthritis or Crohn's disease
EP2359842A1 (en) 2004-07-14 2011-08-24 University of Utah Research Foundation Netrin-related compositions and uses
US20060063208A1 (en) 2004-08-02 2006-03-23 Woolf Clifford J DRG11-responsive (DRAGON) gene and uses thereof
US7517870B2 (en) 2004-12-03 2009-04-14 Fondazione Telethon Use of compounds that interfere with the hedgehog signaling pathway for the manufacture of a medicament for preventing, inhibiting, and/or reversing ocular diseases related with ocular neovascularization
EP1866414B9 (en) 2005-03-31 2012-10-03 Calando Pharmaceuticals, Inc. Inhibitors of ribonucleotide reductase subunit 2 and uses thereof
CA2683559C (en) 2007-04-13 2019-09-24 Dana Farber Cancer Institute, Inc. Methods for treating cancer resistant to erbb therapeutics
EP3486653A1 (en) 2007-05-24 2019-05-22 The United States Government as represented by The Department of Veterans Affairs Treatment of skeletal muscle disorder using ent2
JP2011516065A (en) 2008-04-04 2011-05-26 カランド ファーマシューティカルズ, インコーポレイテッド Compositions and uses of EPAS1 inhibitors
US8470792B2 (en) 2008-12-04 2013-06-25 Opko Pharmaceuticals, Llc. Compositions and methods for selective inhibition of VEGF
IT1398977B1 (en) 2009-06-25 2013-03-28 Icgeb ANTI-PANT AGENT SPECIFIC FOR CEREBRAL CANCERS WITH RECQ1 SUPPRESSION MECHANISM
GB201103167D0 (en) 2011-02-24 2011-04-06 Isis Innovation Gene silencing
EP3738974A1 (en) 2012-09-28 2020-11-18 The University of North Carolina at Chapel Hill Aav vectors targeted to oligodendrocytes
US10300073B2 (en) 2014-10-14 2019-05-28 The Regents Of The University Of California Use of CDK9 and BRD4 inhibitors to inhibit inflammation
CA2975734A1 (en) 2015-02-06 2016-08-11 The University Of North Carolina At Chapel Hill Optimized human clotting factor viii gene expression cassettes and their use
US20180036404A1 (en) 2015-03-02 2018-02-08 180 Therapeutics Lp Method of treating a localized fibrotic disorder using a tnf receptor 2 antagonist
CA3031818A1 (en) 2016-07-27 2018-02-01 Case Western Reserve University Compounds and methods of promoting myelination
WO2018106782A1 (en) 2016-12-08 2018-06-14 Case Western Reserve University Methods and compositions for enhancing functional myelin production
AU2020216480A1 (en) * 2019-01-31 2021-09-09 Oregon Health & Science University Methods for using transcription-dependent directed evolution of AAV capsids

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ROGERS S A ET AL: "ANALYSIS OF CHLOROPLAST PROMOTERS USING BIDIRECTIONAL TRANSCRIPTION VECTORS", PLANT MOLECULAR BIOLOGY, NIJHOFF PUBLISHERS, DORDRECHT, NL, vol. 15, no. 3, 1990, pages 421 - 435, XP002159238, ISSN: 0167-4412 *
SPICER D B ET AL: "AN ANTISENSE PROMOTER OF THE MURINE C-MYC GENE IS LOCALIZED WITHIN INTRON 2", MOLECULAR AND CELLULAR BIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, WASHINGTON, US, vol. 12, no. 3, March 1992 (1992-03-01), pages 1324 - 1329, XP002910389, ISSN: 0270-7306 *
SUREAU ALAIN ET AL: "Characterization of multiple alternative RNAs resulting from antisense transcription of the PR264/SC35 splicing factor gene.", NUCLEIC ACIDS RESEARCH, vol. 25, no. 22, 15 November 1997 (1997-11-15), pages 4513 - 4522, XP002197926, ISSN: 0305-1048 *
VELHAGEN IRIS ET AL: "An antisense promoter within the hepatitis B virus X gene.", INTERVIROLOGY, vol. 38, no. 3-4, 1995, pages 127 - 133, XP008002810, ISSN: 0300-5526 *

Also Published As

Publication number Publication date
WO2001077350A2 (en) 2001-10-18
AU2001253255A1 (en) 2001-10-23

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