WO2001075443A3 - Method and reagents for investigating functional molecular interactions - Google Patents

Method and reagents for investigating functional molecular interactions Download PDF

Info

Publication number
WO2001075443A3
WO2001075443A3 PCT/US2001/010506 US0110506W WO0175443A3 WO 2001075443 A3 WO2001075443 A3 WO 2001075443A3 US 0110506 W US0110506 W US 0110506W WO 0175443 A3 WO0175443 A3 WO 0175443A3
Authority
WO
WIPO (PCT)
Prior art keywords
binding
members
molecular interactions
relates
reagents
Prior art date
Application number
PCT/US2001/010506
Other languages
French (fr)
Other versions
WO2001075443A2 (en
Inventor
Thomas G Consler
John G Gray
Marie A Iannone
Julie B Stimmel
Original Assignee
Glaxo Group Ltd
Thomas G Consler
John G Gray
Marie A Iannone
Julie B Stimmel
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glaxo Group Ltd, Thomas G Consler, John G Gray, Marie A Iannone, Julie B Stimmel filed Critical Glaxo Group Ltd
Priority to EP01922998A priority Critical patent/EP1282818A2/en
Priority to AU2001249738A priority patent/AU2001249738A1/en
Priority to JP2001572869A priority patent/JP2003529767A/en
Publication of WO2001075443A2 publication Critical patent/WO2001075443A2/en
Publication of WO2001075443A3 publication Critical patent/WO2001075443A3/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles

Abstract

The present invention provides a multiplexed assay for analyzing complex molecular interactions. Thus, the invention relates to a method of identifying, in a single assay, the relative binding of two or more first members of a binding pair to one or more second members of a binding pair. The invention also relates to a method of identifying, in a single assay, the relative modulation, by a modulating agent, of binding between two or more binding pairs or between two or more binding complexes. Further provided are methods of screening, in a single assay, for an agent or agents with a selected binding profile or for an agent that modulates binding between two or more first members of a binding pair and one or more second members of a binding pair or the members of two or more binding complexes. The invention also relates to a set of microspheres coupled with a set of cofactors, wherein the microspheres are labeled with a label specific for each cofactor of the set.
PCT/US2001/010506 2000-03-31 2001-03-30 Method and reagents for investigating functional molecular interactions WO2001075443A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP01922998A EP1282818A2 (en) 2000-03-31 2001-03-30 Method and reagents for investigating functional molecular interactions
AU2001249738A AU2001249738A1 (en) 2000-03-31 2001-03-30 Method and reagents for investigating functional molecular interactions
JP2001572869A JP2003529767A (en) 2000-03-31 2001-03-30 Method for testing functional molecular interaction and reagent for use therein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US19382600P 2000-03-31 2000-03-31
US60/193,826 2000-03-31

Publications (2)

Publication Number Publication Date
WO2001075443A2 WO2001075443A2 (en) 2001-10-11
WO2001075443A3 true WO2001075443A3 (en) 2002-11-28

Family

ID=22715172

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/010506 WO2001075443A2 (en) 2000-03-31 2001-03-30 Method and reagents for investigating functional molecular interactions

Country Status (4)

Country Link
EP (1) EP1282818A2 (en)
JP (1) JP2003529767A (en)
AU (1) AU2001249738A1 (en)
WO (1) WO2001075443A2 (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1616179A4 (en) * 2001-12-21 2006-07-12 Centocor Inc A novel screening method for molecular antagonist using flow-cytometry
EP1353182A3 (en) * 2002-04-12 2004-02-04 Smithkline Beecham Corporation Method of predicting cell-based assay results using binding profiles
WO2003087132A2 (en) * 2002-04-12 2003-10-23 Smithkline Beecham Corporation Compositions and methods for evaluating and designing nuclear receptor ligands that modulate co-regulator affinity

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510244A (en) * 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
EP0617286A2 (en) * 1993-03-18 1994-09-28 Wallac Oy Biospecific solid phase carrier
US5858648A (en) * 1996-11-04 1999-01-12 Sienna Biotech, Inc. Assays using reference microparticles
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
WO1999036564A1 (en) * 1998-01-16 1999-07-22 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4510244A (en) * 1980-04-17 1985-04-09 The Board Of Trustees Of The Leland Stanford Jr. University Cell labeling with antigen-coupled microspheres
EP0617286A2 (en) * 1993-03-18 1994-09-28 Wallac Oy Biospecific solid phase carrier
US5891738A (en) * 1995-01-16 1999-04-06 Erkki Soini Biospecific multiparameter assay method
US5858648A (en) * 1996-11-04 1999-01-12 Sienna Biotech, Inc. Assays using reference microparticles
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay
WO1999036564A1 (en) * 1998-01-16 1999-07-22 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods

Also Published As

Publication number Publication date
EP1282818A2 (en) 2003-02-12
JP2003529767A (en) 2003-10-07
WO2001075443A2 (en) 2001-10-11
AU2001249738A1 (en) 2001-10-15

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