WO2001069244A3 - Method for labeling individual cells - Google Patents

Method for labeling individual cells Download PDF

Info

Publication number
WO2001069244A3
WO2001069244A3 PCT/US2001/007785 US0107785W WO0169244A3 WO 2001069244 A3 WO2001069244 A3 WO 2001069244A3 US 0107785 W US0107785 W US 0107785W WO 0169244 A3 WO0169244 A3 WO 0169244A3
Authority
WO
WIPO (PCT)
Prior art keywords
particle
individual cells
labeling
cell
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
PCT/US2001/007785
Other languages
French (fr)
Other versions
WO2001069244A2 (en
Inventor
Jeff W Lichtman
Wai T Wong
Rachel Wong
Wen-Biao Gan
Jamie Grutzendler
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Washington
Washington University in St Louis WUSTL
Original Assignee
University of Washington
Washington University in St Louis WUSTL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Washington, Washington University in St Louis WUSTL filed Critical University of Washington
Priority to AU2001247369A priority Critical patent/AU2001247369A1/en
Priority to JP2001568072A priority patent/JP2003527604A/en
Priority to CA002401270A priority patent/CA2401270A1/en
Priority to EP01920298A priority patent/EP1259808A2/en
Publication of WO2001069244A2 publication Critical patent/WO2001069244A2/en
Publication of WO2001069244A3 publication Critical patent/WO2001069244A3/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
    • A61F13/00—Bandages or dressings; Absorbent pads
    • A61F13/15—Absorbent pads, e.g. sanitary towels, swabs or tampons for external or internal application to the body; Supporting or fastening means therefor; Tampon applicators
    • A61F13/15203—Properties of the article, e.g. stiffness or absorbency
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Vascular Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicinal Preparation (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Orthopedics, Nursing, And Contraception (AREA)
  • Absorbent Articles And Supports Therefor (AREA)

Abstract

A method is provided for the labeling of individual cells. Labeling is accomplished by coating a particle with at least one dye or nucleic acid sequence encoding a marker protein. The particle is then propelled toward the cell resulting in the particle contacting the cell for a time sufficient for the dye or nucleic acid to leave the particle and enter the cell. The present method allows for the differential labeling of individual cells within dense populations of cells.
PCT/US2001/007785 2000-03-10 2001-03-09 Method for labeling individual cells Ceased WO2001069244A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
AU2001247369A AU2001247369A1 (en) 2000-03-10 2001-03-09 Method for labeling individual cells
JP2001568072A JP2003527604A (en) 2000-03-10 2001-03-09 How to label individual cells
CA002401270A CA2401270A1 (en) 2000-03-10 2001-03-09 Method for labeling individual cells
EP01920298A EP1259808A2 (en) 2000-03-10 2001-03-09 Method for labeling individual cells

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18837000P 2000-03-10 2000-03-10
US60/188,370 2000-03-10

Publications (2)

Publication Number Publication Date
WO2001069244A2 WO2001069244A2 (en) 2001-09-20
WO2001069244A3 true WO2001069244A3 (en) 2002-05-02

Family

ID=22692845

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/007785 Ceased WO2001069244A2 (en) 2000-03-10 2001-03-09 Method for labeling individual cells

Country Status (6)

Country Link
US (1) US6869772B2 (en)
EP (1) EP1259808A2 (en)
JP (1) JP2003527604A (en)
AU (1) AU2001247369A1 (en)
CA (1) CA2401270A1 (en)
WO (1) WO2001069244A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9110836B1 (en) 2003-08-22 2015-08-18 Dennis Sunga Fernandez Integrated biosensor and simulation system for diagnosis and therapy
US9582637B1 (en) 2002-10-18 2017-02-28 Dennis Sunga Fernandez Pharmaco-genomic mutation labeling

Families Citing this family (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100425701C (en) * 1999-12-16 2008-10-15 孟山都技术有限公司 Novel Plant Expression Constructs
US6869772B2 (en) * 2000-03-10 2005-03-22 Washington University Method for labeling individual cells
AU2002254029A1 (en) 2001-02-27 2002-09-12 Daniel L. Alkon Alzheimer's disease diagnosis based on mitogen-activated protein kinase phosphorylation
US7361478B2 (en) * 2001-11-20 2008-04-22 Allergan, Inc. High-throughput screen for identifying selective persistent sodium channels channel blockers
WO2005017528A1 (en) * 2003-08-16 2005-02-24 Bayer Healthcare Ag Determination of free fractions
US20070031339A1 (en) 2003-11-28 2007-02-08 Oskar Axelsson Contrast agents
US7683858B2 (en) * 2004-08-02 2010-03-23 Searete Llc Cosmetic enhancement mirror
US7657125B2 (en) * 2004-08-02 2010-02-02 Searete Llc Time-lapsing data methods and systems
US7679581B2 (en) * 2004-08-02 2010-03-16 Searete Llc Medical overlay mirror
US7679580B2 (en) * 2004-08-02 2010-03-16 Searete Llc Time-lapsing mirror
US7714804B2 (en) * 2004-09-15 2010-05-11 Searete Llc Multi-angle mirror
US7876289B2 (en) 2004-08-02 2011-01-25 The Invention Science Fund I, Llc Medical overlay mirror
US9155373B2 (en) 2004-08-02 2015-10-13 Invention Science Fund I, Llc Medical overlay mirror
US20080001851A1 (en) * 2006-06-28 2008-01-03 Searete Llc Cosmetic enhancement mirror
US7636072B2 (en) * 2004-08-02 2009-12-22 Searete Llc Cosmetic enhancement mirror
US7705800B2 (en) * 2004-09-15 2010-04-27 Searete Llc Multi-angle mirror
US7663571B2 (en) * 2004-08-02 2010-02-16 Searete Llc Time-lapsing mirror
JP2008510829A (en) * 2004-08-25 2008-04-10 ザ リージェンツ オブ ザ ユニバーシティ オブ ミシガン Compositions based on dendrimers and their use
US20070041934A1 (en) * 2005-08-12 2007-02-22 Regents Of The University Of Michigan Dendrimer based compositions and methods of using the same
US20070122344A1 (en) 2005-09-02 2007-05-31 University Of Rochester Medical Center Office Of Technology Transfer Intraoperative determination of nerve location
US20090029873A1 (en) 2005-10-11 2009-01-29 Blanchette Rockefeller Neurosciences Institute Alzheimer's Disease-Specific Alterations of the Erk1/Erk2 Phosphorylation Ratio-Alzheimer's Disease-Specific Molecular Biomarkers (Adsmb)
US20080161744A1 (en) 2006-09-07 2008-07-03 University Of Rochester Medical Center Pre-And Intra-Operative Localization of Penile Sentinel Nodes
US20090088376A1 (en) * 2007-04-19 2009-04-02 The Regents Of The University Of Michigan Dendrimer based compositions and methods of using the same
FR2920086A1 (en) * 2007-08-24 2009-02-27 Univ Grenoble 1 ANALYSIS SYSTEM AND METHOD FOR ENDOSCOPY SURGICAL OPERATION
US8406860B2 (en) 2008-01-25 2013-03-26 Novadaq Technologies Inc. Method for evaluating blush in myocardial tissue
WO2009151687A2 (en) 2008-03-12 2009-12-17 The Regents Of The University Of Michigan Dendrimer conjugates
US10219742B2 (en) 2008-04-14 2019-03-05 Novadaq Technologies ULC Locating and analyzing perforator flaps for plastic and reconstructive surgery
EP3372250B1 (en) 2008-05-02 2019-12-25 Novadaq Technologies ULC Methods for production and use of substance-loaded erythrocytes for observation and treatment of microvascular hemodynamics
RU2475537C2 (en) * 2008-05-14 2013-02-20 Новадак Текнолоджиз Инк. Methods of visualisation and composition containing fluorescent dyes bound with virus components for visualisation of nerves
EP2669386A1 (en) 2008-07-28 2013-12-04 Blanchette Rockefeller Neurosciences Institute Stimulus-elicited genomic profile markers of alzheimer's disease
CA2731171C (en) 2008-07-28 2016-10-18 Blanchette Rockefeller Neurosciences Institute Pkc-activating compounds for the treatment of neurodegenerative diseases
WO2010039861A2 (en) 2008-09-30 2010-04-08 The Regents Of The University Of Michigan Dendrimer conjugates
US9017644B2 (en) 2008-11-07 2015-04-28 The Regents Of The University Of Michigan Methods of treating autoimmune disorders and/or inflammatory disorders
US10492671B2 (en) 2009-05-08 2019-12-03 Novadaq Technologies ULC Near infra red fluorescence imaging for visualization of blood vessels during endoscopic harvest
EP2483684B1 (en) * 2009-10-02 2018-04-18 Blanchette Rockefeller Neurosciences, Institute Fibroblast growth patterns to diagnose alzheimer's disease
WO2011059609A2 (en) 2009-10-13 2011-05-19 The Regents Of The University Of Michigan Dendrimer compositions and methods of synthesis
WO2011059586A2 (en) 2009-10-30 2011-05-19 The Regents Of The University Of Michigan Multifunctional small molecules
JP5779837B2 (en) * 2010-02-24 2015-09-16 株式会社Ihi Microorganism detection method
EP2780316B1 (en) 2011-11-13 2020-04-15 Blanchette Rockefeller Neurosciences Institute Esters of dcpla and methods of treatment using the same
WO2013085718A1 (en) 2011-12-08 2013-06-13 The Regents Of The University Of Michigan Multifunctional small molecules
JP6028096B2 (en) 2012-06-21 2016-11-16 ノバダック テクノロジーズ インコーポレイテッド Angiography and perfusion quantification and analysis techniques
CN107209118B (en) 2014-09-29 2021-05-28 史赛克欧洲运营有限公司 Imaging of target fluorophores in biomaterials in the presence of autofluorescence
EP3203902B1 (en) 2014-10-09 2021-07-07 Novadaq Technologies ULC Quantification of absolute blood flow in tissue using fluorescence-mediated photoplethysmography
WO2017214637A1 (en) * 2016-06-10 2017-12-14 Nirvana Sciences Inc. Hydroporphyrin beads with narrow fluorescence emissions
US11904026B2 (en) 2017-02-03 2024-02-20 Nirvana Sciences Inc. Metallohydroporphyrins for photoacoustic imaging
CN110662748A (en) 2017-02-03 2020-01-07 尼尔瓦纳科学股份有限公司 Hydroporphyrins for photoacoustic imaging
CA3049922A1 (en) 2017-02-10 2018-08-16 Novadaq Technologies ULC Open-field handheld fluorescence imaging systems and methods
TWI708050B (en) 2019-07-05 2020-10-21 國立臺灣師範大學 An non-invasive method for evaluating mitochondrial injury of larval fish
JP7709992B2 (en) * 2023-02-17 2025-07-17 Kddi株式会社 Base station device, control method, and program

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5036006A (en) * 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4326524A (en) 1980-09-30 1982-04-27 Minnesota Mining And Manufacturing Company Solid dose ballistic projectile
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5179022A (en) 1988-02-29 1993-01-12 E. I. Du Pont De Nemours & Co. Biolistic apparatus for delivering substances into cells and tissues in a non-lethal manner
US5326692B1 (en) 1992-05-13 1996-04-30 Molecular Probes Inc Fluorescent microparticles with controllable enhanced stokes shift
US5723218A (en) 1990-04-16 1998-03-03 Molecular Probes, Inc. Dipyrrometheneboron difluoride labeled flourescent microparticles
US5204253A (en) 1990-05-29 1993-04-20 E. I. Du Pont De Nemours And Company Method and apparatus for introducing biological substances into living cells
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
EP0706576A1 (en) * 1993-06-30 1996-04-17 Dcv Biologics L.P. A method for introducing a biological substance into a target
US6290991B1 (en) * 1994-12-02 2001-09-18 Quandrant Holdings Cambridge Limited Solid dose delivery vehicle and methods of making same
US5888829A (en) * 1994-11-08 1999-03-30 Molecular Probes, Inc. Photolabile caged ionophores and method of using in a membrane separation process
US5656449A (en) * 1995-03-06 1997-08-12 Molecular Probes, Inc. Neutral unsymmetrical cyanine dyes
US5849526A (en) * 1996-10-15 1998-12-15 The Regents Of The University Of Michigan Use of linalool synthase in genetic engineering of scent production
US6013486A (en) * 1997-09-17 2000-01-11 Yale University Method for selection of insertion mutants
US6869772B2 (en) * 2000-03-10 2005-03-22 Washington University Method for labeling individual cells

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5036006A (en) * 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
GAN WEN-BIAO ET AL: "Multicolor "DiOlistic" labeling of the nervous system using lipophilic dye combinations.", NEURON, vol. 27, no. 2, August 2000 (2000-08-01), pages 219 - 225, XP002190106, ISSN: 0896-6273 *
KETTUNEN P ET AL: "Rapid loading of calcium indicators by particle mediated ballistic delivery.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 27, no. 1, 2001, 31st Annual Meeting of the Society for Neuroscience;San Diego, California, USA; November 10-15, 2001, pages 1008, XP001053553, ISSN: 0190-5295 *
LO D.C. ET AL.: "Neuronal transfection in brain slices using particle-mediated gene transfer", NEURON, vol. 13, 1994, pages 1263 - 1268, XP001053580 *
WONG W T ET AL: "Multicolor diolistic labeling of the nervous system using lipophilic dye combinations.", SOCIETY FOR NEUROSCIENCE ABSTRACTS, vol. 26, no. 1-2, 2000, 30th Annual Meeting of the Society of Neuroscience;New Orleans, LA, USA; November 04-09, 2000, pages Abstract No. - 125.10, XP001053590, ISSN: 0190-5295 *
WU GANG-YI ET AL: "Stabilization of dendritic arbor structure in vivo by CaMKII.", SCIENCE (WASHINGTON D C), vol. 279, no. 5348, 9 January 1998 (1998-01-09), pages 222 - 226, XP002190110, ISSN: 0036-8075 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9582637B1 (en) 2002-10-18 2017-02-28 Dennis Sunga Fernandez Pharmaco-genomic mutation labeling
US9110836B1 (en) 2003-08-22 2015-08-18 Dennis Sunga Fernandez Integrated biosensor and simulation system for diagnosis and therapy
US9111026B1 (en) 2003-08-22 2015-08-18 Dennis Sunga Fernandez Integrated biosensor and simulation system for diagnosis and therapy

Also Published As

Publication number Publication date
JP2003527604A (en) 2003-09-16
EP1259808A2 (en) 2002-11-27
CA2401270A1 (en) 2001-09-20
WO2001069244A2 (en) 2001-09-20
AU2001247369A1 (en) 2001-09-24
US20020155520A9 (en) 2002-10-24
US20010039034A1 (en) 2001-11-08
US6869772B2 (en) 2005-03-22

Similar Documents

Publication Publication Date Title
WO2001069244A3 (en) Method for labeling individual cells
DE69939945D1 (en) METHOD OF ISOLATING STEM CELLS
MX9704765A (en) Improvements in or relating to endometrial function.
EA200000157A1 (en) PREPARATION METHOD FOR CYCLOMETER WITH HIGH EFFICIENT SORTING OF CELLS
WO2003080816A3 (en) Stem cell culture
CA2013582A1 (en) Method for enhancing transmembrane transport of exogenous molecules
WO1995007718A3 (en) Purine nucleoside phosphorylase gene therapy for human malignancy
CA2267668A1 (en) Method for enhancing the efficiency of gene transfer with stem cell factor polypeptide
WO2003089906A3 (en) Functionalized nanoparticles and methods of use
CA2058930A1 (en) Media for cell growth and method for making them
WO2001021760A3 (en) Cell culture spinner flasks
DE60137032D1 (en) USE OF YOUR MARKED CONJUGATES
WO2003068961A3 (en) Method to modify differentiation of pluripotential stem cells
WO2000047762A3 (en) Enriched central nervous system cell populations
WO2004020597A3 (en) Enriched central nervous system stem cell and progenitor cell populations, and methods for identifying, isolating and enriching for such populations
WO2001053518A3 (en) Method of assessing the viability of a cell
AU3696189A (en) Improved composition for isolating and purifying nucleic acid and improved method using same
WO1994026913A3 (en) Marker gene
WO2002008417A1 (en) Process for producing recombinant protein
GB9519776D0 (en) Materials and methods relating to the transfer of nucleic acid into stem cells
WO2003018792A1 (en) Method of detecting toxic substance
WO2002033043A3 (en) Method and marker for the isolation of human multipotent hematopoietic stem cells
Fox Croatian tourism: Consuming culture, affirming identity
WO2001083822A3 (en) Use of representations of dna for genetic analysis
WO2002031143A3 (en) Nucleic acid sequences encoding cmg proteins cmg proteins and methods for their use

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
WWE Wipo information: entry into national phase

Ref document number: 2001247369

Country of ref document: AU

WWE Wipo information: entry into national phase

Ref document number: 2001920298

Country of ref document: EP

WWE Wipo information: entry into national phase

Ref document number: 2401270

Country of ref document: CA

ENP Entry into the national phase

Ref country code: JP

Ref document number: 2001 568072

Kind code of ref document: A

Format of ref document f/p: F

WWP Wipo information: published in national office

Ref document number: 2001920298

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001920298

Country of ref document: EP