WO2001064950A2 - Device and method for preparing a sample for analysis - Google Patents

Device and method for preparing a sample for analysis Download PDF

Info

Publication number
WO2001064950A2
WO2001064950A2 PCT/US2001/006118 US0106118W WO0164950A2 WO 2001064950 A2 WO2001064950 A2 WO 2001064950A2 US 0106118 W US0106118 W US 0106118W WO 0164950 A2 WO0164950 A2 WO 0164950A2
Authority
WO
Grant status
Application
Patent type
Prior art keywords
device
sample
valves
plurality
channels
Prior art date
Application number
PCT/US2001/006118
Other languages
French (fr)
Other versions
WO2001064950A3 (en )
Inventor
Stanley N. Lapidus
Walker M. Sloan, Iii
Original Assignee
Exact Sciences Corporation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date

Links

Classifications

    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02Burettes; Pipettes
    • B01L3/0241Drop counters; Drop formers
    • B01L3/0265Drop counters; Drop formers using valves to interrupt or meter fluid flow, e.g. using solenoids or metering valves
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0605Metering of fluids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00178Special arrangements of analysers
    • G01N2035/00237Handling microquantities of analyte, e.g. microvalves, capillary networks
    • G01N2035/00247Microvalves

Abstract

The invention provides apparatus and methods for dividing a sample, into a plurality of subsamples without the need for pipetting or for other manual operations. Sample is placed, via inlet (2) into sample reservoir (4) in fluid communication with a main charging channel (1) which, in turn, is in fluid communication with a plurality of fractionation channels (5), with valves and vents and terminal purge channel (9). Prior to its use, the plate described above is mated with a receptacle plate, for receiving subsample via fractionation channel valves (6), and to a lid which seals the channels against leakage. The sealed device is subjected to centrifugation in order to distribute sample.

Claims

What is claimed is: 1. A device for fractionating a liquid sample, the device comprising:
A plurality of interconnected channels comprising
a plurality of vents for creating breaks in the channels, thereby to separate a liquid sample into subsamples; and
a plurality of valves for depositing subsamples into receptacles for analysis.
1 2. The device of claim 1 further comprising an inlet for introducing a liquid sample into the device.
1 3. The device of claim 2 further comprising a reservoir in fluid communication with said inlet and at least one of said channels.
1 4. The device of claim 2, wherein said plurality of interconnected channels comprises a primary sample flow channel in fluid communication with a plurality of secondary channels, each of said secondary channels comprising a series of alternating vents for creating a series of vacuum breaks and valves for depositing subsample created
5 by said vacuum breaks.
1 5. The device of claim 2, wherein each member of said plurality of interconnected channels emanate from said inlet.
1 6. The device of claim 4, further comprising a purge channel in fluid communication with said primary sample flow channel, wherein a purge valve separates said purge
3 channel from said sample flow channel.
l 7. The device of claiml , wherein said valves are pressure-activated valves,
l 8. The device of claim 7, wherein said valves are hydrophobic.
1 9. The device of claim 6, wherein said purge valve is activated at a pressure that is
2 different than the pressure necessary to activate said valves.
1 10. The device of claim 1, wherein said channels are etched, machined, stamped, or
2 embossed onto a substrate, and said vents comprise a porous sheet covering said
3 substrate.
1 11.The device of claim 10, wherein said sheet is selected from the group consisting of a
2 Zitex ® sheet and a Goretex® sheet.
12. The device of claim 10, further comprising a lid attached to said substrate for sealing said device.
13. The device of claim 10, further comprising a multi-well receptacle for receiving subsample from said device.
14. The device of claim 13, wherein said substrate comprises a plurality of apertures associated with said valves for depositing subsample into said receptacle.
15. The device of claim 4, further comprising additional main sample flow channels, each associated with at least one secondary channel.
1 16. A device for fractionating a liquid sample, the device comprising: an inlet for introducing liquid sample into the device;
a reservoir in fluid communication with said inlet;
a main sample flow channel in fluid communication with said reservoir
a plurality of secondary channels, each in fluid communication with said main sample flow channel, and each comprising a series of bends;
a plurality of vents for creating vacuum breaks in said secondary channels;
8 a plurality of valves for depositing subsample into a receptacle; and
a purge channel in fluid communication with said main sample flow channel, said 0 purge channel being separated from said main sample flow channel by a valve.
1 17. The device of claim 16, wherein said main sample flow channel is separated from said reservoir by a purge valve.
l 18. The device of claim 16, wherein said valves are pressure-activated valves.
l 19. The device of claim 18, wherein said valves are activated at different pressures.
1 20. The device of claim 17, wherein said purge valve is activated at a different pressure
2 than said valves.
1 21.A method for fractionating a liquid sample, the method comprising the steps of:
2 introducing a liquid sample into a device according to claim 1;
-14- applying pressure to said device, thereby to cause sample to move through the device; and
activating said vents, thereby to separate said sample into subsamples by creating a plurality of vacuum breaks.
1 22. The method of claim 21 , further comprising the step of depositing said subsamples into a receptacle which receptacle can be either on the device or mounted in
3 proximity to it.
l 23. The method of claim 22, further comprising the step of analyzing said subsamples.
1 24. The method of claim 21 , wherein said applying and activating steps comprise
2 rotating said device.
1 25. The method of claim 22, wherein said depositing step comprises rotating said
2 device.
3 26. The method of claim 22, wherein said depositing step comprises applying pressure
4 to liquid in said channels, thereby to activate said vents.
l 27. The method of claim 21 , wherein said sample is a biological sample.
l 28. The method of claim 21 , wherein said sample is a non-biological sample.
1 29. The method of claim 23, wherein said analyzing step comprises conducting one or
2 more polymerase chain reaction.
-15-
PCT/US2001/006118 2000-02-28 2001-02-27 Device and method for preparing a sample for analysis WO2001064950A3 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US51455900 true 2000-02-28 2000-02-28
US09/514,559 2000-02-28

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP20010914503 EP1261739A2 (en) 2000-02-28 2001-02-27 Device and method for preparing a sample for analysis
AU3988601A AU3988601A (en) 2000-02-28 2001-02-27 Device and method for preparing a sample for analysis
CA 2401819 CA2401819A1 (en) 2000-02-28 2001-02-27 Device and method for preparing a sample for analysis

Publications (2)

Publication Number Publication Date
WO2001064950A2 true true WO2001064950A2 (en) 2001-09-07
WO2001064950A3 true WO2001064950A3 (en) 2002-01-24

Family

ID=24047724

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/006118 WO2001064950A3 (en) 2000-02-28 2001-02-27 Device and method for preparing a sample for analysis

Country Status (3)

Country Link
EP (1) EP1261739A2 (en)
CA (1) CA2401819A1 (en)
WO (1) WO2001064950A3 (en)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002041995A1 (en) * 2000-11-27 2002-05-30 Pyrosequencing Ab Microfluidic flow control
US6818404B2 (en) 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338666A (en) * 1991-01-23 1994-08-16 Becton, Dickinson And Company Method for distributing a liquid sample into a multiple aliquot device
EP0803288A2 (en) * 1996-04-26 1997-10-29 Kyoto Daiichi Kagaku Co., Ltd. Device and method for analyzing a sample
US5798215A (en) * 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
WO1999058245A1 (en) * 1998-05-08 1999-11-18 Gyros Ab Microfluidic device
WO1999060397A1 (en) * 1998-05-18 1999-11-25 University Of Washington Liquid analysis cartridge

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5338666A (en) * 1991-01-23 1994-08-16 Becton, Dickinson And Company Method for distributing a liquid sample into a multiple aliquot device
US5798215A (en) * 1993-02-18 1998-08-25 Biocircuits Corporation Device for use in analyte detection assays
EP0803288A2 (en) * 1996-04-26 1997-10-29 Kyoto Daiichi Kagaku Co., Ltd. Device and method for analyzing a sample
WO1999058245A1 (en) * 1998-05-08 1999-11-18 Gyros Ab Microfluidic device
WO1999060397A1 (en) * 1998-05-18 1999-11-25 University Of Washington Liquid analysis cartridge

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6818404B2 (en) 1997-10-23 2004-11-16 Exact Sciences Corporation Methods for detecting hypermethylated nucleic acid in heterogeneous biological samples
US7981612B2 (en) 1999-12-07 2011-07-19 Mayo Foundation For Medical Education And Research Methods of screening for supracolonic neoplasms based on stool samples containing a nucleic acid marker indicative of a neoplasm
WO2002041995A1 (en) * 2000-11-27 2002-05-30 Pyrosequencing Ab Microfluidic flow control
US6911308B2 (en) 2001-01-05 2005-06-28 Exact Sciences Corporation Methods for detecting, grading or monitoring an H. pylori infection
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US9777314B2 (en) 2005-04-21 2017-10-03 Esoterix Genetic Laboratories, Llc Analysis of heterogeneous nucleic acid samples

Also Published As

Publication number Publication date Type
CA2401819A1 (en) 2001-09-07 application
WO2001064950A3 (en) 2002-01-24 application
EP1261739A2 (en) 2002-12-04 application

Similar Documents

Publication Publication Date Title
US4569647A (en) Apparatus for preparing and embedding tissue samples for histological examination
US6395536B2 (en) Sample processing device with a chamber forming member
US20030129614A1 (en) Apparatus and method for isolating a nucleic acid from a sample
US7011794B2 (en) Upon a cartridge for containing a specimen sample for optical analysis
US5591636A (en) Membrane holder
US5647994A (en) Method and apparatus for separating magnetic particles from a solution
US20070087431A1 (en) Cassette for sample preparation
US4576702A (en) Analytical electroelution device
US4342341A (en) Blood transfer device
US20030143124A1 (en) Unidirectional flow control sealing matt
US20100152895A1 (en) Shock freezer and system for preparing samples for analysis using the same
US6286375B1 (en) Apparatus for facilitating headspace sampling
US5529694A (en) Solid phase extraction apparatus and method of using same
US5957822A (en) Lid for closing vessels
US5217619A (en) Liquid-solid extraction apparatus and method of using same
US20050048670A1 (en) Biological specimen collection and analysis system
US4957706A (en) Device for preserving sterility during sampling from culture medium containers
US4409814A (en) Gas extracting device
Skopec et al. Analysis of organophosphorus pesticides in rice by supercritical fluid extraction and quantitation using an atomic emission detector
US6017698A (en) Method of binding a biological material
WO2005011867A3 (en) Processing particle-containing samples
WO2001073395A3 (en) Method of preventing short sampling of a capillary or wicking fill device
US20060060531A1 (en) Method and apparatus for directly sampling a fluid for microfiltration
US6199436B1 (en) Method and apparatus for field fluid sampling and dissolved gas analysis
US4873876A (en) Chemical process sampler

Legal Events

Date Code Title Description
AK Designated states

Kind code of ref document: A2

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A2

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

121 Ep: the epo has been informed by wipo that ep was designated in this application
DFPE Request for preliminary examination filed prior to expiration of 19th month from priority date (pct application filed before 20040101)
AK Designated states

Kind code of ref document: A3

Designated state(s): AU CA JP

AL Designated countries for regional patents

Kind code of ref document: A3

Designated state(s): AT BE CH CY DE DK ES FI FR GB GR IE IT LU MC NL PT SE TR

WWE Wipo information: entry into national phase

Ref document number: 2001239886

Country of ref document: AU

Ref document number: 2401819

Country of ref document: CA

WWE Wipo information: entry into national phase

Ref document number: 2001914503

Country of ref document: EP

WWP Wipo information: published in national office

Ref document number: 2001914503

Country of ref document: EP

WWW Wipo information: withdrawn in national office

Ref document number: 2001914503

Country of ref document: EP

NENP Non-entry into the national phase in:

Ref country code: JP