WO2001059154A3 - Process for characterising nucleic acids in solution - Google Patents

Process for characterising nucleic acids in solution Download PDF

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Publication number
WO2001059154A3
WO2001059154A3 PCT/GB2001/000492 GB0100492W WO0159154A3 WO 2001059154 A3 WO2001059154 A3 WO 2001059154A3 GB 0100492 W GB0100492 W GB 0100492W WO 0159154 A3 WO0159154 A3 WO 0159154A3
Authority
WO
WIPO (PCT)
Prior art keywords
solution
process
nucleic acids
parameter
characterising nucleic
Prior art date
Application number
PCT/GB2001/000492
Other languages
French (fr)
Other versions
WO2001059154A2 (en
Inventor
Benjamin David Cobb
Original Assignee
Molecular Sensing Plc
Benjamin David Cobb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to GBGB0002859.7A priority Critical patent/GB0002859D0/en
Priority to GB0002859.7 priority
Application filed by Molecular Sensing Plc, Benjamin David Cobb filed Critical Molecular Sensing Plc
Publication of WO2001059154A2 publication Critical patent/WO2001059154A2/en
Publication of WO2001059154A3 publication Critical patent/WO2001059154A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804Nucleic acid analysis using immunogens
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/02Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating the impedance of the material
    • G01N27/04Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating the impedance of the material by investigating resistance
    • G01N27/06Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating the impedance of the material by investigating resistance of a liquid

Abstract

The invention provides a method for ascertaining information relating to nucleic acid material in a solution, comprising: measuring a parameter relating to the conductivity of the solution at a multiplicity of temperatures; analysing the measurements of said parameter; and detecting a discontinuity in the change of said parameter with temperature.
PCT/GB2001/000492 2000-02-08 2001-02-07 Process for characterising nucleic acids in solution WO2001059154A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
GBGB0002859.7A GB0002859D0 (en) 2000-02-08 2000-02-08 Process for characterising nucleic acids in solution
GB0002859.7 2000-02-08

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU32019/01A AU3201901A (en) 2000-02-08 2001-02-07 Process for characterising nucleic acids in solution

Publications (2)

Publication Number Publication Date
WO2001059154A2 WO2001059154A2 (en) 2001-08-16
WO2001059154A3 true WO2001059154A3 (en) 2002-03-21

Family

ID=9885162

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/GB2001/000492 WO2001059154A2 (en) 2000-02-08 2001-02-07 Process for characterising nucleic acids in solution

Country Status (3)

Country Link
AU (1) AU3201901A (en)
GB (1) GB0002859D0 (en)
WO (1) WO2001059154A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100858080B1 (en) 2002-11-12 2008-09-10 삼성전자주식회사 A method for detecting a PCR product by measuring a electrical signal
KR100571822B1 (en) * 2003-10-30 2006-04-17 삼성전자주식회사 Micro PCR device, method for amplifying a nucleic acid and method for measuring concentration of PCR product using the same

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2248301A (en) * 1990-09-12 1992-04-01 Ici Plc Apparatus and method for the detection of changes in the composition of a material
EP0543550A1 (en) * 1991-11-19 1993-05-26 Houston Advanced Research Center Method and apparatus for molecule detection
US5403451A (en) * 1993-03-05 1995-04-04 Riviello; John M. Method and apparatus for pulsed electrochemical detection using polymer electroactive electrodes
WO1997032039A1 (en) * 1996-02-29 1997-09-04 Molecular Sensors Limited Method for quantitative determination of a nucleic acid in solution by measurement of the electrical conductivity and apparatus therefor
WO1999010530A1 (en) * 1997-08-22 1999-03-04 Molecular Sensors Limited Estimation of nucleic acid
WO1999037819A2 (en) * 1998-01-27 1999-07-29 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
WO2001040511A2 (en) * 1999-12-01 2001-06-07 The Secretary Of State For Defence Detection system

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2248301A (en) * 1990-09-12 1992-04-01 Ici Plc Apparatus and method for the detection of changes in the composition of a material
EP0543550A1 (en) * 1991-11-19 1993-05-26 Houston Advanced Research Center Method and apparatus for molecule detection
US5403451A (en) * 1993-03-05 1995-04-04 Riviello; John M. Method and apparatus for pulsed electrochemical detection using polymer electroactive electrodes
WO1997032039A1 (en) * 1996-02-29 1997-09-04 Molecular Sensors Limited Method for quantitative determination of a nucleic acid in solution by measurement of the electrical conductivity and apparatus therefor
WO1999010530A1 (en) * 1997-08-22 1999-03-04 Molecular Sensors Limited Estimation of nucleic acid
WO1999037819A2 (en) * 1998-01-27 1999-07-29 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
WO2001040511A2 (en) * 1999-12-01 2001-06-07 The Secretary Of State For Defence Detection system

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BONE STEPHEN ET AL: "Dielectric studies of intermolecular interactions in native DNA.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1306, no. 1, 1996, pages 93 - 97, XP001016208, ISSN: 0006-3002 *
BONE STEPHEN ET AL: "Dielectric studies of ion fluctuation and chain bending in native DNA.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1260, no. 1, 1995, pages 85 - 93, XP001016207, ISSN: 0006-3002 *
LEE R S ET AL: "DIELECTRIC STUDIES OF CHAIN MELTING AND DENATURATION IN NATIVE DNA", BIOCHIMICA ET BIOPHYSICA ACTA. GENE STRUCTURE AND EXPRESSION, ELSEVIER, AMSTERDAM, NL, vol. 1397, 11 May 1998 (1998-05-11), pages 316 - 324, XP000972743, ISSN: 0167-4781 *

Also Published As

Publication number Publication date
AU3201901A (en) 2001-08-20
WO2001059154A2 (en) 2001-08-16
GB0002859D0 (en) 2000-03-29

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