WO2001059149A3 - Peptide biosensors for anthrax protease - Google Patents

Peptide biosensors for anthrax protease Download PDF

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Publication number
WO2001059149A3
WO2001059149A3 PCT/US2001/004253 US0104253W WO0159149A3 WO 2001059149 A3 WO2001059149 A3 WO 2001059149A3 US 0104253 W US0104253 W US 0104253W WO 0159149 A3 WO0159149 A3 WO 0159149A3
Authority
WO
WIPO (PCT)
Prior art keywords
protease
anthrax protease
peptide biosensors
biosensors
peptide
Prior art date
Application number
PCT/US2001/004253
Other languages
French (fr)
Other versions
WO2001059149A2 (en
Inventor
Sarah Burroughs-Tencza
Original Assignee
Cellomics Inc
Sarah Burroughs-Tencza
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellomics Inc, Sarah Burroughs-Tencza filed Critical Cellomics Inc
Priority to US10/182,303 priority Critical patent/US7410769B2/en
Priority to EP01937142A priority patent/EP1354201A2/en
Priority to CA002398583A priority patent/CA2398583A1/en
Priority to AU2001262906A priority patent/AU2001262906A1/en
Publication of WO2001059149A2 publication Critical patent/WO2001059149A2/en
Publication of WO2001059149A3 publication Critical patent/WO2001059149A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56911—Bacteria
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/32—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Bacillus (G)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The present invention provides a protease biosensor that can be used to detect the presence of the lethal factor protease from Bacillus anthracis, as well as methods for using the protease biosensor.
PCT/US2001/004253 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease WO2001059149A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US10/182,303 US7410769B2 (en) 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease
EP01937142A EP1354201A2 (en) 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease
CA002398583A CA2398583A1 (en) 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease
AU2001262906A AU2001262906A1 (en) 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US18201100P 2000-02-11 2000-02-11
US60/182,011 2000-02-11

Publications (2)

Publication Number Publication Date
WO2001059149A2 WO2001059149A2 (en) 2001-08-16
WO2001059149A3 true WO2001059149A3 (en) 2003-08-21

Family

ID=22666731

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2001/004253 WO2001059149A2 (en) 2000-02-11 2001-02-09 Peptide biosensors for anthrax protease

Country Status (5)

Country Link
US (2) US20020076741A1 (en)
EP (1) EP1354201A2 (en)
AU (1) AU2001262906A1 (en)
CA (1) CA2398583A1 (en)
WO (1) WO2001059149A2 (en)

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Publication number Priority date Publication date Assignee Title
US20030096315A1 (en) 2000-05-03 2003-05-22 Sanders Mitchell C. Device for detecting bacterial contamination and method of use
ES2271078T3 (en) * 2000-05-03 2007-04-16 Expressive Constructs, Inc. METHOD FOR DETECTING LYSTERY MONOCYTOGENES.
AU2002230415A1 (en) 2000-11-17 2002-05-27 Universal Imaging Corporation Rapidly changing dichroic beamsplitter
US6972326B2 (en) * 2001-12-03 2005-12-06 Molecular Probes, Inc. Labeling of immobilized proteins using dipyrrometheneboron difluoride dyes
GB0200479D0 (en) * 2002-01-09 2002-02-27 Medical Res Council Fluorogenic protease substrates
US9017963B2 (en) 2002-01-31 2015-04-28 Woundchek Laboratories (Us), Inc. Method for detecting microorganisms
US6955891B2 (en) 2002-02-25 2005-10-18 Merck & Co., Inc. Reagents for assaying Bacillus anthracis lethal factor protease
WO2003073066A2 (en) * 2002-02-25 2003-09-04 Merck & Co., Inc. Reagents and methods for assaying bacillus anthracis lethal factor protease
US9987185B1 (en) 2002-02-27 2018-06-05 CAMS Medical Instruments, Inc. Transducer devices, apparatus, systems and methods of operation
US9463332B2 (en) * 2002-02-27 2016-10-11 CAMS Medical Instruments, Inc. Personal tuner with biosensor and bioscanner
US7183066B2 (en) 2002-09-27 2007-02-27 Allergan, Inc. Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins
DE10329860A1 (en) 2003-07-02 2005-01-20 Atto-Tec Gmbh Sulfonamide derivatives of polycyclic dyes for analytical applications
EP1692510B1 (en) 2003-11-03 2008-01-02 Ethicon, Inc. Methods, peptides and biosensors useful for detecting a broad spectrum of bacteria
GB0329161D0 (en) * 2003-12-16 2004-01-21 Precisense As Reagant for detecting an analyte
US20070130713A1 (en) * 2005-12-14 2007-06-14 Kimberly-Clark Worldwide, Inc. Cleaning wipe with textured surface
WO2007136436A2 (en) * 2006-02-15 2007-11-29 The Government Of The United States Of America, As Represented By The Secretary, Department Of The Health And Human Services, Centers For Disease Control And Prevention Detection of anthrax pathogenicity factors
US8003314B2 (en) 2007-04-16 2011-08-23 Diagnostic Hybrids, Inc. Methods for direct fluorescent antibody virus detection in liquids
US20110195442A1 (en) * 2008-10-17 2011-08-11 Sailaja Chandrapati Sterility indicating biological compositions, articles and methods
EP2366027B1 (en) 2008-11-20 2017-01-11 Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO Rapid fret-based diagnosis of bacterial pathogens
EP2475778B1 (en) 2009-09-09 2014-10-22 3M Innovative Properties Company Methods and kit for protease enzyme assays
US10246492B2 (en) 2009-10-16 2019-04-02 Biomadison, Inc. Botulinum assay with synaptobrevin substrate moiety
WO2014193237A2 (en) 2013-05-30 2014-12-04 Academisch Ziekenhuis Leiden H.O.D.N. Lumc C diff protease
US9932570B2 (en) 2015-10-23 2018-04-03 List Biological Laboratories, Inc. Anthrax lethal factor substrates and methods of use thereof
WO2018044540A1 (en) * 2016-08-31 2018-03-08 Regeneron Pharmaceuticals, Inc. Compositions and methods for detecting protein trafficking
WO2018190713A1 (en) 2017-04-12 2018-10-18 Umc Utrecht Holding B.V. Inhibitors of lysine methyltransferase for treatment of pain
WO2019118837A1 (en) * 2017-12-15 2019-06-20 The Regents Of The University Of Colorado, A Body Corporate Synthetic fluorescent protein biosensors and use thereof in drug screening methods

Citations (6)

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WO1994028166A1 (en) * 1993-05-27 1994-12-08 Zeneca Limited Fluorescence energy transfer substrates
WO1997040065A2 (en) * 1996-04-24 1997-10-30 Peptide Therapeutics Limited Substrates and inhibitors of proteolytic enzymes
WO1998037226A1 (en) * 1997-02-20 1998-08-27 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
US5981200A (en) * 1996-01-31 1999-11-09 The Regents Of The University Of California Tandem fluorescent protein constructs
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000073437A1 (en) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins

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US5589452A (en) * 1992-07-14 1996-12-31 Syntex (U.S.A.) Inc. Analogs of parathyroid hormone and parathyroid hormone related peptide: synthesis and use for the treatment of osteoporosis
US5605809A (en) * 1994-10-28 1997-02-25 Oncoimmunin, Inc. Compositions for the detection of proteases in biological samples and methods of use thereof
US5989835A (en) * 1997-02-27 1999-11-23 Cellomics, Inc. System for cell-based screening
US6103479A (en) * 1996-05-30 2000-08-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
EP1840220A3 (en) 1998-04-01 2007-10-24 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the Secretary of the Department of Health and Human Services Anthrax lethal factor is a MAPK kinase protease
US6955891B2 (en) * 2002-02-25 2005-10-18 Merck & Co., Inc. Reagents for assaying Bacillus anthracis lethal factor protease

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994028166A1 (en) * 1993-05-27 1994-12-08 Zeneca Limited Fluorescence energy transfer substrates
US5981200A (en) * 1996-01-31 1999-11-09 The Regents Of The University Of California Tandem fluorescent protein constructs
WO1997040065A2 (en) * 1996-04-24 1997-10-30 Peptide Therapeutics Limited Substrates and inhibitors of proteolytic enzymes
WO1998037226A1 (en) * 1997-02-20 1998-08-27 Oncoimmunin, Inc. Compositions for the detection of enzyme activity in biological samples and methods of use thereof
WO2000050872A2 (en) * 1999-02-26 2000-08-31 Cellomics, Inc. A system for cell-based screening
WO2000073437A1 (en) * 1999-05-27 2000-12-07 Merck Frosst Canada & Co. Assays for caspase activity using green fluorescent proteins

Non-Patent Citations (3)

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Title
DATABASE SWISS-PROT "MEK1 (AN: Q02750), MEK2 (AN: P36507), MPK6 (AN: P52564)", XP002213804 *
G. VITALE ET AL: "Susceptibility of mitogen-activated protein kinase kinase family members to proteolysis by anthrax lethal factor", BIOCHEMISTRY JOURNAL, vol. 352, - 1 December 2000 (2000-12-01), pages 739 - 745, XP002213803 *
G. VITALE, R. PELLIZZARI, C. RECCHI, G. NAPOLITANI, M. MOCK & C. MONTECUCCO: "Anthrax lethal factor cleaves the N-terminus of MAPKKs and induces tyrosine/threonine phosphorylation of MAPKs in cultured macrophages", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 248, 1998, pages 706 - 711, XP002213802 *

Also Published As

Publication number Publication date
US20030166028A1 (en) 2003-09-04
US20020076741A1 (en) 2002-06-20
AU2001262906A1 (en) 2001-08-20
US7410769B2 (en) 2008-08-12
EP1354201A2 (en) 2003-10-22
WO2001059149A2 (en) 2001-08-16
CA2398583A1 (en) 2001-08-16

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