WO2001051508A1 - Peptide inhibitors of lfa-1/icam-1 interaction - Google Patents

Abstract

Cyclic peptides inhibit LFA-1 interaction with ICAM-1 and are useful in treatment of hematopoietic neoplasms and in adjunct therapy in prevention of retinoic acid syndrome and diseases involving emigration of leukocytes into organ tissue.

Description

PEPTIDE INHIBITORS OFLFA-1/TCAM-1 INTERACTION

BACKGROUND

1. Field of the Invention

The present invention relates to the field of biologically active peptide containing compositions for use in the prevention and treatment of hematopoietic neoplastic diseases, particularly leukemia.

2. Description of Related Art

LFA-1 (lymphocyte function associated antigen- 1) is an integrin αβ heterodimer (Carlos and Harlan, 1994; Springer, 1994; Larson and Springer, 1990; McEver, 1990; Picker and Butcher, 1992). Although three other integrins restricted in expression to leukocytes share the same β subunit and have homologous α subunits (Mac-1, pl50,95, and alpha d), only LFA-1 is expressed on normal and leukemia T cells (Larson and Springer, 1990). LFA-1 binds ICAM-1 (intracellular adhesion molecule), and although LFA-1 is constitutively expressed on all leukocytes, LFA-1 binding to ICAM-1 requires cellular activation. Activation, in part, results in conformational changes in LFA-1 that affect its avidity for ICAM-1. In contrast, ICAM-1 is constitutively avid and expressed on a wide array of cell types including leukocytes, endothelium, stromal cells, and fibroblasts. In a model developed by the present inventor, a stromal cell derived soluble factor cooperates with LFA-1 on the surface of T lineage acute lymphoblastic leukemia (T-ALL) cells (Winter et al, 1998). The LFA-1 on T-ALL cells results in bone marrow (BM) stromal cell binding via ICAM-1 that leads to enhanced leukemia cell survival. Furthermore, aberrant LFA-l/ICAM-1 dependent interaction between circulating leukemia cells and endothelial cells lining blood vessels promotes extravasation of leukemia cells into tissue as seen in the life-threatening therapeutic complication of acute leukemia, retinoic acid syndrome (Brown et al, 1999). Hence, the development of effective in vivo inhibitors of LFA-1/ICAM interaction would be useful in the therapy of acute leukemia and prevention of therapeutic complications. The present inventor has shown, for example, that inhibition of LFA-l/ICAM-1 dependent stromal cell binding with mAbs decreases survival of T-ALL cell lines and T-ALL cells isolated from patients. In one study, a representative sample from a patient with T-ALL showed that survival of T-ALL cells is augmented by BM stromal cells and that survival is inhibited by mAbs directed against LFA-1 (mAb TSI/22,5 μg/ml) or its ligand ICAM-1 (mAb 84H10, 10 μg/ml). This observation has been replicated for T-ALL cell lines Jurkat and Sup T I as well as a subset of patients with T-ALL. However, even though in vivo use of mAbs against LFA-1 or ICAM-1 blocks LFA-1 function in a number of disease models, unfortunately anaphylactic reactions and secondary physiologic effects have hampered this approach (McMuray, 1996; DeMeester: et al, 1996; Jackson et al, 1997; Cuthbertson et al, 1997; Gundel et al, 1992; Harning et al, 1993; Nakano et al, 1995).

Another means to interfere with protein-protein interactions is through the use of small peptide inhibitors. In fact, small peptide inhibitors to adhesion molecules structurally-related to LFA-1 have recently been approved for clinical use in coagulopathies (Ohman et al, 1995; Adgey et al, 1998; Leficovis and Topol, 1995). Short linear peptides (<30 amino acids) have also been described that prevent or interfere with integrin dependent firm adhesion using sequences derived from integrin or their ligands. In particular, these peptides have been derived from a number of integrin receptors: the β2 and β3 subunits of integrins, and the αjjb subunit of ICAM-1, and VCAM-1 (Murayama et al, 1996; Jacobsson and Frykberg, 1996; Zhang and Plow, 1996; Budnik et al, 1996; Vanderslice et al, 1997; Suehiro et al, 1996; Endemann et al, 1996). However, the clinical applicability of these linear peptides is limited. The half maximal inhibitory concentration (IC₅₀; concentration at which aggregation is inhibited 50%) for most of these peptides is 10^"4 M with purified receptor-ligand pairs (univalent interactions) and they are ineffective at inhibiting multivalent interactions, during cell-cell adhesion. In addition, linear peptides have short serum half-lives because of proteolysis. Therefore, prohibitively high concentrations of peptide would have to be administered in a clinical setting and a biologic effect would not necessarily occur. Longer peptides, ranging in length from 25-200 residues, have also been reported to block βl, β2, and β3 integrin dependent adhesion (Zhang and Plow, 1996; Budnik et al, 1996; Vanderslice et al, 1997; Suehiro et al, 1996; Endeman et al, 1996). In general, these peptide inhibitors may have higher affinities or slower off-rates than short peptides and, therefore, are better inhibitors. However, they are still susceptible to proteolysis.

Therefore, a need exists to develop novel and specific classes of pharmaceutical agents to inhibit the binding of LFA-1 and ICAM-1 and to be useful in the treatment of hematopoietic neoplastic diseases as well as other diseases that involve emigration of leukocytes from blood into tissue, such as myocardial infarction, radiation injury, asthma, rheumatoid arthritis, and lymphoma metastasis.

SUMMARY

The present invention addresses the problems in the art by providing compositions that include cyclic peptide inhibitors of binding interactions between the integrin, lymphocyte function associated antigen- 1 (LFA-1) expressed on leukocytes, including leukemic T-cells, and intracellular adhesion molecule 1 (ICAM-1), expressed on a variety of cell types. As stated above, this binding of activated LFA-1 is implicated in a variety of diseases and inhibition of this binding interaction with a cyclic peptide inhibitor will have implications in the treatment or management of those diseases.

As a part of the present invention, phage display has been used to identify peptide sequences that bind ICAM-1 and block LFA-1/ICAM interaction. Phage that specifically bound ICAM-1 were identified by repeated selection from a cysteine-constrained heptapeptide phage display library. The peptide sequences expressed on ICAM-1 binding phage were determined by nucleotide sequencing. A consensus sequence, CLLRMRSIC (SEQ ID NO: ) was derived from the analysis of the most frequently occurring sequences. Analysis of less frequently recurring amino acids of ICAM-1 binding-phage identified variants of SEQ ID NO: 1 wherein the second amino acid is methionine (SEQ ID NO: 17), or in which the 5^th amino acid is proline (SEQ ID NO: 5), or in which the 6^th amino acid is asparagine (SEQ ID NO: 9), or in which the 7^th amino acid is leucine (SEQ ID NO: 3), or in which the 8^th amino acid is arginine (SEQ ID NO: 2), or any combination of these substitutions.

Another aspect of the present disclosure is the application of an alanine screening technique to the consensus sequence, SEQ ID NO: 1. An alanine was substituted at each position in the hepatapeptide LLRMRSI of the consensus sequence SEQ ID NO: 1, and each peptide was examined in for its ability to inhibit LFA-l/ICAM-1 mediated cell aggregation. Alanine substitution of the isoleucine at position 8 of SEQ ID NO: 1, i.e., CLLRMRSAC (SEQ ID NO: 40), resulted in a more potent antagonist. Loss of inhibitory function resulted from alanine substitution of the leucines in positions 2 (SEQ ID NO: 34) and 3 (SEQ ID NO: 35), methionine in position 5 (SEQ ID NO: 37), and arginine in position 6 (SEQ ID NO: 38) of SEQ ID NO: 1, indicating that these amino acids are important to the antagonistic activity of the peptide. Substitution of the serine in position 7 (SEQ ID NO: 39) had no significant effect on the inhibitory activity of the peptide. Substitution of the arginine in position 4 (i.e., SEQ ID NO: 36) of SEQ ID NO: 1 was not soluble in aqueous solution at 1 nm and was not tested.

A further aspect of the present invention is the use of conservative amino acid substitutions of the key amino acid residues identified by the alanine screen. Sequences that exhibit greater inhibition of LFA-l/ICAM-1 mediated cell inhibition as compared to SEQ ID NO: 1, include CILRMRSAC (SED ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO 45), CLLRMKSAC (SEQ ID NO: 46), and CLLRMRSVC (SEQ ID NO: 48).

The present invention may be described, therefore, in certain aspects as a composition comprising a cyclic peptide inhibitor of LFA-l/ICAM-1 interaction, wherein the composition has the amino acid sequence, CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof. Conservative variants are described elsewhere herein, and include the exchange of an amino acid for another of like charge, size, or hydrophobicity, and include, but are not limited to, CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SED ID NO: 44), CLLKMRSAC (SEQ ID NO 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48). The present disclosure would also include variants of SEQ ID NO: 1 in which the 3^rd and 4^th amino acids remain the same and other amino acids of the sequence are substituted and tested empirically for their ability to inhibit the LFA-l/ICAM-1 interaction. The amino acid sequence is numbered in the conventional sense, in that the first amino acid on the N-terminus is cysteine, followed by 7 amino acids and then a carboxy terminal cysteine. In the practice of the invention, the two terminal cysteines may form a disulfide bonded cystine residue resulting in a cyclic peptide as is well known in the art. Alternatively, the terminal cysteines may be linked by an amide peptide linkage, either directly or separated by one or more amino acids, leaving two free sulfhydryl groups.

Based on the empirical data obtained by the inventor and disclosed herein, a cyclic peptide of the invention may have the sequence of SEQ ID NO: 1, or it may be a derivative of that sequence in which the second amino acid is methionine (SEQ ID NO: 17), or in which the 5^th amino acid is proline (SEQ ID NO: 5), or in which the 6^th amino acid is asparagine (SEQ ID NO: 9), or in which the 7^h amino acid is leucine (SEQ ID NO: 3), or in which the 8^th amino acid is arginine (SEQ ID NO: 2), or any combination of these substitutions, or even conservative variants of any of these substitutions.

Another aspect of the present invention includes cyclic peptides inhibitors of LFA-l/ICAM-1 interaction comprising the heptapeptide sequences which include LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SED ID NO: 54) or LLRMRSV (SEQ ID NO: 55). Peptides that comprise the heptapeptide sequences of the present invention may be a peptide of 7, or 8, or 9, or 10, or 11, or 12, or 13, or 14, or 15 amino acids in length, wherein additional amino acids may be any L- series or any D-series amino acid. In preferred embodiments of the invention, the peptides or peptide mimetics of the invention exhibit an inhibition constant (IC₅₀) for the binding interaction of LFA-l/ICAM-1 of from about 10 μM to about 800 μM for cell aggregation or from about 10 to about 250 nM for monovalent ICAM-1 binding. The term "IC₅₀" is well known in the art, and means the half maximal inhibitory concentration, or concentration at which aggregation is inhibited by 50%.

Any of the compositions described herein may be formulated for pharmacological or therapeutic administration either to a mammal, or more preferably to a human. As such, the compositions may be contained in a pharmaceutically acceptable carrier. The preferred mode of administration of a peptide active agent is by injection, either intravenous, intra-arterial, intramuscular or subcutaneous. Other routes of administration may also be possible and would be included within the scope of the present disclosure.

The compositions may be administered parenterally or intraperitoneally.

Solutions of the active compounds as free base or pharmacologically acceptable salts can be prepared in water suitably mixed with a surfactant, such as hydroxypropylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.

The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases the form must be sterile and must be suitably fluid. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms, such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyethylene glycol, and the like), suitable mixtures thereof, and vegetable oils. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, by the maintenance of the required particle size in the case of dispersion and by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In many cases, it will be preferable to include isotonic agents, for example, sugars or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.

Sterile injectable solutions are prepared by incorporating the active compounds in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, the preferred methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.

As used herein, "pharmaceutically acceptable carrier" includes any and all solvents, dispersion media, coatings, antibacterial and antifungal agents, isotonic and absorption delaying agents and the like. The use of such media and agents for pharmaceutical active substances is well known in the art. Except insofar as any conventional media or agent is incompatible with the active ingredient, its use in the therapeutic compositions is contemplated. Supplementary active ingredients can also be incorporated into the compositions.

A peptide can be formulated into a composition in a neutral or salt form. Pharmaceutically acceptable salts, include the acid addition salts (formed with the free amino groups) and which are formed with inorganic acids such as, for example, hydrochloric or phosphoric acids, or such organic acids as acetic, oxalic, tartaric, mandelic, and the like. Salts formed with the free carboxyl groups can also be derived from inorganic bases such as, for example, sodium, potassium, ammonium, calcium, or ferric hydroxides, and such organic bases as isopropylamine, trimemylamine, histidine, procaine and the like.

For parenteral administration in an aqueous solution, for example, the solution should be suitably buffered if necessary and the liquid diluent first rendered isotonic with sufficient saline or glucose. These particular aqueous solutions are especially suitable for intravenous, intramuscular, subcutaneous and intraperitoneal adnώiistration. In this connection, sterile aqueous media which can be employed will be known to those of skill in the art in light of the present disclosure. For example, one dosage could be dissolved in 1 mL of isotonic NaCI solution and either added to lOOOmL of hypodermoclysis fluid or injected at the proposed site of infusion, (see for example, "Remington's Pharmaceutical Sciences" 15th Edition, pages 1035-1038 and 1570-1580). Some variation in dosage will necessarily occur depending on the condition of the subject being treated. The person responsible for admimstration will, in any event, determine the appropriate dose for the individual subject.

It is an aspect of the present disclosure that the disclosed compositions may be used in adjunct therapy in standard treatments for diseases such as hematopoietic neoplasms. The present invention may be described therefore, in certain embodiments as a method of preventing retinoic acid syndrome in a subject receiving all-trans retinoic acid, comprising administering to the subject an effective amount of a composition comprising a cyclic peptide, wherein the peptide comprises a heptapeptide sequence including, but not limited to, LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) or LLRMRSV (SEQ ID NO: 55). In some embodiments the cyclic peptide has the amino acid sequence CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof, such as CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48). An embodiment of the invention may also be described as a method for inhibiting growth of leukemia cells comprising preventing an LFA-l/ICAM-1 interaction between the leukemia cells and support cells such as bone marrow stromal cells, wherein the method comprises contacting the leukemia cells with a cyclic peptide inhibitor of the LFA-l/ICAM-1 interaction, and further wherein the amino acid sequence of the cyclic peptide inhibitor is not a fragment of the amino acid sequence of LFA-1 or ICAM-1. In the practice of this method, the leukemia cells are preferably in a leukemia patient and contacting comprises administering the cyclic peptide to the patient.

An embodiment of the invention is also a therapeutic package for dispensing to, or for use in dispensing to, a mammal or human being treated for a hematopoietic neoplastic disease, myocardial infarction, radiation injury, asthma, rheumatoid arthritis, or lymphoma metastasis, wherein the package contains in a unit dose, an amount of a cyclic peptide comprising a heptapeptide sequence including, but not limited to, LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) or LLRMRSV (SEQ ID NO: 55), effective to inhibit an LFA-l/ICAM-1 interaction in a subject when administered periodically. In some embodiments the cyclic peptide has the amino acid sequence CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof, such as CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48). In certain embodiments a unit dose is from about 10 to about 500 μg/Kg, or is from about 50 to about 250 μg/Kg, or from about 120 to about 150 μg/Kg. The unit dose may be an initial bolus dose which may be followed by a continuous infusion of about 5 to about 250 μg/Kg/min, or from about 40 to about 60 μg/Kg/min, or may be about 50 μg/Kg/min. Alternatively, unit doses may be repeated daily, and administered multiple times per day. Dosage regimens are not limited to those exemplified, and the invention encompasses any dosage regimen that delivers an therapeutically effective dose. An example of clinical administration of a peptidomimetic inhibiting an integrin functions is docum ented by Ohman et al (1995). An embodiment of the present disclosure is a method of inhibiting emigration of leukocytes from blood into tissue in a subject comprising administering to the subject an amount of a cyclic peptide comprising a heptapeptide sequence including, but not limited to, LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) or LLRMRSV (SEQ ID NO: 55), effective to inhibit an LFA-l/TCAM-1 interaction in the subject. In some embodiments the cyclic peptide has the amino acid sequence, CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof, such as CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48). In preferred embodiments the subject is susceptible to the development of or is suffering from a hematopoietic neoplastic disease, myocardial infarction, radiation injury, asthma, rheumatoid arthritis, or lymphoma metastasis.

A further embodiment of the invention is a method comprising a competitive binding assay for screening the ability of candidate compounds to bind to ICAM-1. The method comprises assessing the displacement of candidate compounds by cyclic petide inhibitors of LFA-l/ICAM-1 binding interaction. The cyclic peptide may comprise a heptapeptide sequence including, but not limited to, LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) or LLRMRSV (SEQ ID NO: 55). In some embodiments the cyclic peptide has the amino acid sequence, CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof, such as CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO; 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48).

Other embodiments of the present disclosure include methods of therapy relating to tissue allografts such as renal, heart and thryoid allografts, bone marrow transplants, diabetes, rheumatoid arthritis, psoriasis, T-cell mediated sensitization reactions such as contact sensitization, and other T-cell mediated disorders. Such methods comprise comprising administering to the subject an amount of a cyclic peptide comprising a heptapeptide sequence including, but not limited to, LLRMRSI (SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) or LLRMRSV (SEQ ID NO: 55), effective to inhibit an LFA-l/ICAM-1 interaction in the subject. In some embodiments the cyclic peptide has the amino acid sequence, CLLRMRSIC (SEQ ID NO: 1) or CLLRMRSAC (SEQ ID NO: 40), or a conservative variant thereof, such as CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), or CLLRMRSVC (SEQ ID NO: 48).

BRIEF DESCRIPTION OF THE DRAWINGS

The following drawings form part of the present specification and are included to further demonstrate certain aspects of the present invention. The invention may be better understood by reference to one or more of these drawings in combination with the detailed description of specific embodiments presented herein.

FIG. 1 is a bar graph display of data showing aggregation of JY cells being inhibited by either mAbs against LFA-1, or by titration of 100 μM to 1 mM of the consensus peptide SEQ ID NO: 1. A randomized peptide, CLMIRMLRC (SEQ ID NO: 33) at a concentration of ImM is shown to be ineffective.

FIG. 2 represents the structure of a carboxy fluorescein derivative of the disulfide constrained cyclic form of SEQ ID NO: 46. DETAILED DESCRIPTION

Hematopoietic neoplasms including acute leukemia constitute 7% of male and 6% of female cancer (Ries et al, 1994). If untreated, the clinical course of acute leukemia is rapidly fatal. Drugs to treat acute leukemia usually interfere with cell replication, and as a result, these drugs have non-specific physiologic consequences and undesirable side effects. One of the fundamental problems in the development of novel leukemia therapies is the identification of pharmaceutical targets and the production of pharmaceutical agents against those targets. The present inventor has shown that LFA-1 and ICAM-1 are molecular targets for use in the therapy of acute leukemia using two assays that recapitulate the molecular and cellular events leading to leukemia cell proliferation in the marrow and spread of leukemia cells to distant sites. The first assay simulates the marrow microenvironment. In this assay, T lineage acute lymphoblastic leukemia (T-ALL) cells isolated from patients are co-cultured with bone marrow (BM) stromal cells that support hematopoiesis. T-ALL cells isolated from patients will die in ex vivo culture, and BM stromal cells have been shown to support ex vivo survival of T-ALL cells. Furthermore, in co-culture experiments mAbs to LFA-1 and ICAM-1 dramatically decrease T-ALL cell survival by inhibiting adherence to bone marrow stromal cells. This latter finding indicates that survival of T-ALL cells in the marrow microenvironment is dependent on proper LFA-l/ICAM-1 binding. Since failure to eradicate leukemia cells in the marrow is a major reason for treatment failure, adjunct therapy that included inhibition of LFA-1 /ΪC AM- 1 ligation between leukemia cells and T-ALL cells may cause T-ALL cell death and improved patient outcome. This may have applicability to all T-cell leukemias.

The present inventor has also established a model and dissected the mechanism of a life-threatening therapeutic complication of acute promyelocytic leukemia (APL), retinoic acid syndrome. All-tr rø retinoic acid (ATRA) promotes a complete remission in up to 80% of patients with APL. However, retinoic acid syndrome occurs in up to 30% of patients and is caused by APL cells infiltrating organs after retinoic acid therapy. Using an apparatus known as a parallel plate flow chamber, the interactions that occur in blood vessels between circulating leukemia cells and endothelial cells have been recapitulated under physiologic flow conditions (Brown et al, 1999; Larson et al, 1997). Upregulation of LFA-1 activity on APL cells after retinoic acid treatment results in APL cell binding to and transmigration through endothelium, analogous to the organ infiltration seen in APL patients being treated with retinoic acid. mAbs against LFA-1 and ICAM-1 completely inhibit the binding to and transmigration through endothelium. Hence, inhibition of LFA-1/ICAM interaction in patients with APL being treated with retinoic acid may protect against the development of retinoic acid syndrome. LFA- 1/ICAM inhibition is also applicable to the use of alternatives to retinoic acid, such as 9- cis-retinoic acid or various synthetic retinoids, in APL treatment, to the extent that they cause retinoic acid syndrome.

Inhibition of the LFA-1 and ICAM-1 binding has potential therapeutic benefits relating to blocking allograft rejection allografts, including cardiac, renal and thryoid allografts (Isobe et al, 1992; Stepkowski et al, 1994; Cosimi et al, 1990; Nakakura et al, 1993; Talento et al, 1993), bone marrow transplants (Tibbetts et al, 1999; Cavazzana-Calvo et al, 1995) T-cell mediated sensitization reactions (Ma et al, 1994; Cumberbatch et al, 1992), diabetes (Hasegawa et al, 1994) and rheumatoid arthritis (Davis et al, 1995; Kavanaugh et al, 1994). Expression of ICAM-1 by keratinocytes is also implicated in the etiology of psoriasis, and inhibition of LFA-l/ICAM-1 binding presents a possible point of therapeutic intervention (Servitje et al, 1996). Thus the peptide compositions of the present invention may be used in treatment of the above conditions and more generally in any condition T-cell mediated condition wherein T-cells are activated via interaction of LFA-1 and ICAM-1.

The present disclosure provides novel peptide containing compositions that inhibit the LFA-l/ICAM-1 interaction. Preferred are compositions including nine amino acid peptides in which the terminal amino acids are cysteines, thus allowing the peptide to exist in a heterodetic cyclic form by disulfide bond formation or in a homodetic form by amide peptide bond formation between the terminal amino acids. Cyclizing small peptides through disulfide or amide bonds between the N- and C-terminus cysteines may circumvent problems of affinity and half-life. Disulfide bonds connecting the amino and carboxy terminus decrease proteolysis and also increase the rigidity of the structure, which may yield higher affinity compounds. Peptides cyclized by disulfide bonds have free amino- and carboxy-terrmni which still may be susceptible to proteolytic degradation, while peptides cyclized by formation of an amide bond between the N- terminal amine and C-terminal carboxyl, no longer contain free amino or carboxy termini. Cyclic peptides may have longer half-lives in serum (Picker and Butcher, 1992; Huang et al., 1997). Moreover, the side-effects from peptide therapy are minimal, since anaphylaxis and immune responses against the small peptide occur only rarely (Ohman et al, 1995; Adgey, 1998). Finally cyclic peptides have been shown to be effective inhibitors in vivo of integrins involved in human and animal disease (Jackson et al, 1997; Cuthbertson et al, 1997; Leficovis and Topol, 1995; Goligorsky et al, 1998; Ojima et al, 1995; Noiri et al, 1994). Thus the peptides of the present invention, including CLLRMRSIC (SEQ ID NO: 1), CLLRMRSAC (SEQ ID NO: 40), CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), and CLLRMRSVC (SEQ ID NO: 48), can be linked either by a C-N linkage or a disulfide linkage.

The present invention is not limited by the method of cyclization of peptides, but encompasses peptides whose cyclic structure may be achieved by any suitable method of synthesis. Thus, heterodetic linkages may include, but are not limited to formation via disulfide, alkylene or sulfide bridges. Methods of synthesis of cyclic homodetic peptides and cyclic heterodetic peptides, including disulfide, sulfide and alkylene bridges, are well known in the art. Some methods are disclosed in U.S. Patent No. 5,643,872. Other examples of cyclization methods are discussed and disclosed in U.S. Patent No.

6,008,058. Cyclic peptides can also be prepared by incorporation of a type II' β-turn dipeptide (Doyle et al, 1996). In certain aspects, embodiments of the present invention include cyclic peptides comprising the heptapeptides represented by residues 2 through 8 of the exemplified cysteine-containing nonapeptides. Thus, embodiments of the present invention include cyclic peptides comprising the heptapeptide sequences LLRMRSI

(SEQ ID NO: 49), LLRMRSA (SEQ ID NO: 50), LIRMRSA (SEQ ID NO: 51), LLKMRSA (SEQ ID NO: 52), LLRMKSA (SEQ ID NO: 53), ILRMRSA (SEQ ID NO: 54) and LLRMRSV (SEQ ID NO: 55). Peptides that comprise the heptapeptide sequences of the present invention may be a peptide of 7, or 8, or 9, or 10, or 11, or 12, or 13, or 14, or 15 amino acids in length, wherein additional amino acids may be any L- series or any D-series amino acid.

Phage Display and Consensus Sequence

As a part of the present disclosure, phage display has been used to identify peptide sequences that bind ICAM-1 and block LFA-1 /TCAM interaction. Briefly a library of cysteine-constrained heptapeptides was purchased from New England Labs (Cambridge, MA) and screened for its ability to bind the LFA-1 ligand, ICAM-1. Human ICAM-1 has been previously isolated in functional form (Larson et al, 1990), and a variation of this technique was used to obtain purified recombinant soluble ICAM-1 for use in phage display. Each phage in the library has the potential to display a unique cyclic heptapeptide fused to its gene III coat on its surface. The linkage of the displayed random peptide with a phage surface protein forms the basis of the technique. The library consists of approximately 2.8 x 10¹¹ random heptapeptide sequences expressed on phage, compared to 20⁷ (20 possible amino acids in 7 different positions) or 1.28 x 10⁹ possible heptapeptide sequences. The phage were then screened for their ability to bind purified ICAM-1 by interaction with the displayed heptapeptide sequences. The phage were then screened for their ability to bind purified ICAM-1 by panning, wherein a library of phage, each displaying a different peptide sequence is applied to a surface coated with ICAM-1. Unbound phage is washed away. Bound phage is eluted using the anti-ICAM-1 mAb 84h410. This mAb binds to amino acid residues on ICAM-1 that are similar to those to which LFA-1 binds (Staunton et al, 1990). Elution with R6.5 allowed for isolation of phage expressing cyclic peptides that bind a region on ICAM-1 that is shared with LFA-1 binding. In addition, phage were eluted with mAb for 1 hour so that the peptides with highest affinity and slower off-rates (i.e., peptides most likely to be potent in vivo inhibitors) would be included. Thus, peptide sequences that block ICAM-l/LFA-1 interactions are identified. Adherent phage were selected and amplified in ER2537 bacteria through four rounds of panning. The sequences of 12-18 phage in each round were determined. A working consensus peptide was determined after nucleotide sequencing of 18 phage in the fourth and final round (Table 1). Amino acids that were recurring but occurred at lower frequency and did not fit into the consensus sequence are also shown below. The recurring amino acids form the basis of derivative structures. The ability of each phage isolated after four rounds of panning to specifically bind ICAM-1 was also determined in an ELISA assay with serial dilutions of phage.

TABLE 1. PEPTIDE SEQUENCES EXPRESSED ON PHAGE THAT BIND ICAM-1

The following techniques have been developed or modified for use in the present examples described below.

Rapid aggregation assay for screening peptide effectiveness.

LFA-1 dependent cell aggregation has been previously studied using an aggregation assay with a variety of leukocyte subclasses and cell lines (Larson et al, 1990; Wang et al, 1988; Larson et al, 1997). JY cells were obtained form American Type Tissue Culture Collection and were maintained in RPMI 1640 supplemented with 10%) FBS at 37°C in 5% C0₂. Aggregation of cells was measured in a homotypic aggregation assay using ICAM-1 as a stimuli. JY cells were washed twice with serum free medium and resuspended at a concentration of 4 x 105 cells/ml. Cells were preincubated with the desired concentration of peptide for 15 min at room temperature. In a final volume of 100 μl, 50μl of cells and peptide were seeded in 96 well flat bottomed microtiter plates. Cells were allowed to aggregate at 37°C in humidified air with 5% C0₂. Cells were visualized and counted by inverted phase contrast microscopy at the time indicated. Within each well of aggregates as well as the total number of free (single) cells were counted, percent aggregation was determined by the following equation:

Percent aggregation = 100 x (1 -number of free cells/total number of cells)

Assay for measuring LFA-1 dependent ex vivo survival of leukemic cells.

A co-culture assay has been developed by the present inventor that quantifies ex vivo survival of T-ALL cells (Winter et al, 1998). Using this assay, survival of T-ALL cell lines as well as T-ALL cells isolated from patients required LFA-1 binding to ICAM-1 on bone marrow (BM) derived stromal cells. In this assay cryopreserved or fresh leukemic samples are seeded onto HS5 stromal cell monolayers in 24 well plates. The stromal cell line HS5 has been previously shown to support complete hematopoiesis of normal precursor cells (Roecklein and Torak-Storb, 1995). HS5 cells are γ-irradiated with 2500 cGy, a dose that has been determined to prevent stromal cell proliferation over 168 hours. Leukemia cells are harvested at 1 and 96 hours. The number of leukemic cells recovered is measured in a flow cytometer by techniques based on those known in the art (Manabe et al, 1992; Manabe et al, 1994). The leukemia cells are stained by direct immunofluorescence using a fluoroisothiocyanate (FITC) labeled mAb directed against a pan T-ALL antigen CD5 as described (Larson et al, Leukocyte Typing, 1990; Larson and McCurley, 1995). A gate is set around the area of light scatter where the viable CD5 positive T-ALL cells are found at the beginning of the cultures. Then, the T-ALL cells with predetermined light scattering and CD5 presentation are enumerated by counting the number of events passing through the gate in a 60 second time period. In each analysis 5 x 10⁵ fluorescent Immuno-Chek beads (Coulter, Hialeah, FL) are added to each sample. The number of beads that pass through the flow cytometer in 60 seconds is also counted, allowing the measured bead number to serve as an internal control for the volume that passes through the flow cytometer in 60 seconds. The calculation is as follows: (number of CD5 T-ALL cells/volume passed through flow cytometer as determined by fluorescent beads) X volume of the sample = the number of cells in the sample. The percentage of survival is calculated by: (number of cells in test sample at t-96 h/number of cell in sample at t-1 h) x 100. Parallel plate flow chamber for measuring leukocyte-endothelial cell interaction under physiologic flow conditions in vivo. An assay has been developed that provides an in vitro model of neutrophils or

APL cells binding to activated endothelium. The binding of APL cells using a parallel plate flow chamber recapitulates events that occur in retinoic acid syndrome (Larson et al, 1997; Brown et al, 1999). A parallel plate flow chamber simulates the physiologic flow conditions in blood and adhesive interactions in post-capillary venules. Post-capillary venules are the physiologically relevant locations of leukemia cell-endothelial cell interaction and extravasation. Since parallel plate flow chamber experiments have been shown to accurately recapitulate in vivo observations, a parallel plate flow chamber is used to examine the inhibitory effects of peptides on APL cell line binding and transmigration through endothelium under physiologic flow conditions. Monolayers of endothelial cells are placed in the parallel plate flow chamber, and the leukemic cells are pumped through the chamber at physiologic flow rates. The interaction between the flowing leukemia cells and the endothelium are videotaped microscopically, and the number of rolling, firmly adhered and transmigrated leukemia cells is quantified by computer-assisted image analysis.

With all-trans retinoic acid (ATRA) treatment, the APL cell line NB-4 acquires the ability to firmly attach to activated endothelium via LFA-l/ICAM-1 interaction. Inhibition of LFA-l/ICAM-1 interaction prevents firm adherence to and transmigration through endothelium of the APL cell line under physiologic flow. This has been demonstrated with monoclonal antibodies against LFA-1 and ICAM-1, which prevent firm attachment to and transmigration through activated endothelium of APL cells in a parallel plate flow chamber (Larson et al, 1997; Brown et al, 1999). Flowing ATRA-treated APL cell lines over activated endothelial cell monolayers in a parallel flow chamber determines the effectiveness of peptides to inhibit LFA-1 dependent firm adherence and subsequent transmigration under physiologic flow conditions. ICAM-1 expressed on activated endothelial monolayers are incubated with cyclic peptides over a range of concentrations (10^"4 to 10^"8 M) and the IC₅₀ is deteπnined. For investigating neutrophil binding, neutrophils are isolated from heparin anticoagulated venous blood of healthy adult donors by centrifugation on Ficoll-Hypaque density gradients as described by Simon et al. (1992). Isolated neutrophils are suspended at a concentration of lOVml in Hanks' Balanced Salt Solution supplemented with 10 mmol/L HEPES, pH 7.4 and 0.2% human serum albumin (Armour Pharmaceutical, Kankakee, IL) and used within 2 hours of preparation. Neutrophils are kept on ice and resuspended in RPMt pre- warmed to 37°C immediately before use.

Monoclonal antibodies

In order to have adequate monoclonal antibodies at a reasonable cost, hybridomas were grown by the present inventor and mAbs were purified for blocking studies. The following monoclonal antibodies have been isolated from hybridoma supernatants: mAbs against LFA-1 (TS2/4 and TSI/22) and ICAM-1 (RRI/1, R6.5 and 84H10) (Larson et al, 1997).

Screening Assay

The ability of candidate compounds to bind to ICAM-1 is assessed by a competitive binding assay. Candidate compounds may be peptide or non-peptide compounds. Binding to ICAM-1 is quantified by the ability to displace a peptide of the present invention, including the peptides CLLRMRSIC (SEQ ID NO: 1), CLLRMRSAC (SEQ ID NO: 40), CILRMRSAC (SEQ ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO: 45), CLLRMKSAC (SEQ ID NO: 46), and CLLRMRSVC (SEQ ID NO: 48), from ICAM-1. The displaced peptide can be assayed by a number of techniques. For example, radiolabeled peptide can be synthesized using commercial available radiolabeled amino acids precursors. Peptides radiolabelled with H³, C¹⁴ or S³³ can be quantified by routine liquid scintillation techniques. Alternatively, a fluorescent labeled peptide can be synthesized. For example, lysine can be inserted in a non-critical position and labeled with fluroescein isothiocyanate ("FITC"). In addition to FITC, the peptide may be labeled with any suitable flurophore. A carboxy fluroescein derivative of SEQ ID NO: 1 has been prepared. The 5(6)-carboxy fluorescein derivative of the disulfide constrained cyclic form of SEQ ID NO: 40, as shown by FIG. 3, has been synthesized by Biopeptide (San Deiego, CA): Alternatively, peptides cyclized with an amide peptide linkage have free sulfhydryl groups available for linkage to fluorescent compounds such as thiocyanates. Separation of bound from unbound peptide and quantitation of displaced peptide can be performed by routine techniques known to one of skill in the art. This embodiment of the invention is not limited by the method used to quantify the displaced peptide, and any suitable analytical technique may be used and be within the scope of the invention.

* * *

The following examples are included to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventor to function well in the practice of the invention, and thus can be considered to constitute preferred modes for its practice. However, those of skill in the art should, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain a like or similar result without departing from the spirit and scope of the invention.

EXAMPLE 1

Consensus Sequence Peptide Blocks LFA-l/ICAM-1 Dependent Cell

Aggregation

The consensus peptide (SEQ ID NO: 1) and the randomized sequence CLMIRMLRC (SEQ ID NO: 33), cyclizied in the disulfide-linked form, were synthesized by Biopeptide (San Diego, CA) and tested in a screening assay for the ability to inhibit aggregation of the cell line JY, which aggregates in an LFA-1 /CAM- 1 dependent manner. Aggregation measurements are relatively rapid and quantifiable. Furthermore, small volumes are used rninimizing peptide consumption. Finally, cell aggregation in vitro depends on a multivalent LFA-l/ICAM-1 interaction. A peptide that is identified as being able to block cell aggregation in vitro is, therefore, likely to have affinity and off-rate characteristics that would make it an effective in vivo inhibitor, since cell-cell interaction in vivo also involves multivalent LFA-1 /ICAM-1 interaction. Using this assay, the consensus peptide sequence has an IC₅₀ of 500μM (FIG. 1).

EXAMPLE 2 Derivative Peptide Antagonists

The consensus peptide sequence (SEQ ID NO: 1) identified by phage display and described in Example 1 has an IC₅₀ Of 800 μM for cell aggregation and 250 nM for monovalent ICAM binding. Monovalent ICAM-1 binding is measured by standard ELISA techniques, wherein, for example, the inhibition of 84H10 binding to ICAM-1 is assayed. For in vivo use, preferred peptides would have IC₅₀'s of about 10 μM to 800 μM for cell aggregation and from about 10 to 250 nM for monovalent ICAM binding. In order to obtain more effective inhibitory peptides, standard techniques of designing derivative structures may be used to produce candidates to be tested for enhanced inhibitory capacity. This strategy has been shown to be effective in developing in vivo peptide inhibitors to other adhesion molecules (Ohman et al, 1995; Adgey, 1998).

For example, heptapeptide sequences are proposed on the basis of the observed consensus sequence of different phage sequences that were discovered in the phage display studies described above. Two of the amino acid positions in the consensus sequence show greater than 80% identity among phage and therefore are fixed (positions 3 and 4 of Table 1). The other positions are altered using 1 of 2 possible amino acids as shown in Table 2. The amino acids employed in the altered positions are identical to the recurring amino acids identified by phage display, but are represented at too low a frequency to be incorporated in the consensus sequence peptide. Using these combinations, 32 cysteine restrained heptapeptides are available as candidate inhibitors.

Peptides are initially tested for their ability to block LFA-1/ICAM dependent aggregation of the cell line JY (Wang et al, 1988; Larson et al, 1997). Peptides are preferably tested over a range of concentrations (10¹ to 10⁸ μM, for example) and the concentration leading to half maximal inhibition of aggregation (IC50) is determined for all the peptide sequences.

TABLE 2. Peptide Antagonists Identified by Phage Display

Alanine Screen Of Consensus Sequence

An alternative derivative peptide strategy was undertaken in which the consensus sequence peptide was subjected to an alanme-screening procedure as described by Cunningham and Wells, 1989. Peptides cycUzied in the disulfide-linked form were synthesized by Biopeptide (San Diego, CA) wherein an alanine was substituted at each position in the hepatapeptide LLRMRSI of the consensus sequence SEQ ID NO: 1, and each peptide was examined in parallel for its ability to inhibit LFA-l/ΪCAM-1 mediated JY cell aggregation. The results are shown in Table 2. Six of the seven peptides were soluble in aqueous solution. SEQ ID NO: 36, representing a substitution of the arginine in position 4 of SEQ ID NO: 1, was not soluble at 1 nm and could not be tested. Loss of inhibitory function resulted from alanine substitution of the leucines in positions 2 (SEQ ID NO: 34) and 3 (SEQ ID NO: 35), methionine in position 5 (SEQ ID NO: 37), and arginine in position 6 (SEQ ID NO: 38) of SEQ ID NO: 1, indicating that these amino acids are important to the antagonistic activity of the peptide. Substitution of the serine in position 7 (SEQ ID NO: 39) had no significant effect on the inhibitory activity of the peptide. Alanine substitution of the isoleucine at position 8 of SEQ ID NO: 1, i.e., CLLRMRSAC (SEQ ID NO: 40), resulted in a more potent antagonist. The randomized sequence CLRLSRIMC (SEQ ID NO: 56) was used as a control. This randomized sequence has a greater aqueous solubility than the alternative randomized sequence CLMIRMLRC (SEQ ID NO: 33). Conservative substitutions in other peptide antagonists been shown to result in more potent inhibitors (Jennings and White, 1998).

TABLE 3 Inhibition of JY cell aggregation with alanine-substituted derivations

(SD) in parentheses

* = Randomized cyclic peptide

Conservative Subtitutions Of Amino Acids

The important amino acids of SEQ ID NO: 40, as identified by the alanine substitutions, were substituted with amino acids of like charge, e.g., as described by

Dayoff et al, Atlas of Protein Sequence and Structure; vol 5, Suppl. 3, pp3,45-362 (M.O. Dayoff, ed., Nat'l BioMed Research Fdn., Washington, D.C. 1979). Peptides cyclizied in the disulfide-linked form were synthesized by Biopeptide (San Diego, CA) and were examined for their ability to inhibit LFA-l/ICAM-1 mediated JY cell aggregation. The results are summarized in Table 4. Five peptide sequences exhibited greater inhibition of JY cell inhibition as compared to SEQ ID NO: 1, i.e., CILRMRSAC (SED ID NO: 43), CLIRMRSAC (SEQ ID NO: 44), CLLKMRSAC (SEQ ID NO 45), CLLRMKSAC (SEQ ID NO: 46), and CLLRMRSVC (SEQ ID NO: 48). This type of derivative strategy has previously been shown to be successful at identifying higher affinity peptides (Ohman et al, 1995; Adgey, 1998). In addition to binding to ICAM, conservative varaints can also be screened for other desirable properties such as a longer serum-half life or desirable other pharmacokinetic of pharmacodymanic properties.

TABLE 4 Inhibition of JY cell aggregation with homologous amino acid substitutions

(SD) in parentheses

* = Randomized cyclic peptide EXAMPLE 3 Investigation Of Peptide Inhibition Of LFA-l ICAM-1 Interaction

The cyclic peptide inhibitors having the amino acid sequences, CLLRMRSIC (SEQ ID NO: 1) and CLLRMKSAC (SEQ ID NO: 46) have been shown to block neutrophil-binding to endothelium under physiologic flow conditions in a parallel plate flow chamber. The data is shown in Table 5. In similar fashion to the inhibition of JY cell aggregation, SEQ ID NO: 46 exhibited greater inhibition of attachment of neutrophils to activated endothelium.

TABLE 5

Inhibition of Firm Attachment of Neutrophils to Stimulated Endothelial Cells in a Parallel Plate Flow Chamber

Percent inhibition = ( 1 -(Observed/Maximum)) x 100 (SD) in parentheses * = Randomized cyclic peptide

The peptides of the present invention bind to ICAM-1 and block cell-cell adhesion that is dependent on LFA-1 binding to its ligand, ICAM-1. In contrast to other peptide inhibitors, these compounds have no structural similarity to fragments or portions of LFA-1 or ICAM-1. These compound are, therefore, effective agents in the treatment of myocardial infarction, rheumatoid arthritis, asthma, and leukemia/lymphoma, and are contemplated to be especially effective in adjunct therapy of acute T-ALL and retinoic acid syndrome in APL. Regarding the clinical utility of the disclosed compositions, preliminary data indicate that approximately 80% of cases of T-ALL may utilize LFA-1 dependent adhesion for survival. Therefore, the peptide inhibitor may be useful in 80% of cases of T-ALL when incorporated as adjunct therapy. Side effects related to use of a small peptide are contemplated to be minimal, since peptidomimetics inhibiting other integrin functions, and now in clinical trial do not have significant side-effects (Ohman et al., 1995; Adgey, 1998). However, LFA-1 may be critical to an aspect of hematopoiesis that is as yet undefined.

All of the compositions and methods disclosed and claimed herein can be made and executed without undue experimentation in light of the present disclosure. While the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those of skill in the art that variations may be applied to the compositions and/or methods and in the steps or in the sequence of steps of the methods described herein without departing from the concept, spirit and scope of the invention. More specifically, it will be apparent that certain agents that are chemically or physiologically related may be substituted for the agents described herein while the same or similar results would be achieved. All such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the spirit, scope and concept of the invention as defined by the appended claims.

REFERENCES

The following references, to the extent that they provide exemplary procedural or other details supplementary to those set forth herein, are specifically incorporated herein by reference.

Adgey, "An Overview of the Results of Clinical Trials With Glycoprotein IIB/IIIa inhibitors," Amer Heart J 135:S43, 1998.

Anderson and Springer, "Leukocyte adhesion deficiency: An inherited defect in the Mac- 1, and pl50, 95 glycoproteins," Ann Rev Med, 38:175, 1987.

Bargatze et al, "Distinct roles of L-selectin and integrins a4B7 and LFA-1 in lymphocyte homing to Peyer's patch-HEV in situ: the multistep model confirmed and refined," Immunity, 3:99, 1995.

Belhadj Jrad and Bahraoui, "Antigenicity of linear and cyclic peptides mimicking the disulfide oops I HIV-2 envelope glycoprotein: synthesis, reoxidation, and purification," J Peptide Res, 51:370, 1998.

Brown et al, "All-trans retinoic acid regulates adhesion mechanism and transmigration of the acute promyelocytic leukemia cell line NB-4 under physiologic flow," Brit J Haematol, 107:86,1999.

Budnik et al, "Analysis of the production of soluble ICAM-1 molecules by human cells," Exp Hematol, 24:3 52, 1996.

Carlos and Harlan, "Leukocyte-endothelial adhesion molecules," Blood, 84:2068, 1994.

Cavazzana-Calvo et al, prevention of bone marrow and cardiac graft rejection in an H-2 haplotype disparate mouse combination by an anti-LFA-1 antibody," Transplantation, 59:1576, 1995.

Ciufetti et al, "Neuto hil-derived adhesion molecules in human digital ischaemia and reperfusion", Vasa, 24:155, 1995.

Cosimi et al, In vivo effects of monoclonal antibody to ICAM-1 (CD54) in nonhuman primates with renal allografts," J Immunol, 144:4604, 1990.

Cox, "The Analysis of Binary Data", Methuen, London, 1970.

Cumberbatch et al, "Adhesion molecule expression by epidermal Langerhans cells and lymph node dendritic cells: a comparison," Arch. Dermatol Res., 288:739, 1996. Cunningham and Wells, "High-resolution epitope mapping of hHGH-receptor interactions by alanine-scanning mutagenesis," Science, 244:1801, 1989.

Cuthbertson et al, "Design of low molecular weight hematoregulatory agents from the structure-activity relationship of a dimeric pentapeptide," J Medicinal Chem 40:2876, 1997.

Davies et al, Induction of persistent T cell hyporesponsiveness in vivo by monoclonal antibody to ICAM-1 in patients with rheumatoid arthritis," J Immunol, 154:3525, 1995.

DeMeester et al, "Attenuation of rat lung isograft reperfiision injury with a combination of anti-ICAM-1 and anti-beta2 integrin monoclonal antibodies," Transplantation 62:1477, 1996.

Diamond and Springer, "The dynamic regulation of integrin adhesiveness," Curr Biol, 4:506, 1994.

Doyle et al, "Solution structure of a biologically active cyclic LDV peptide analogue containing a type IF β-turn mimetic," Int. J. peptide Protein Res. 47:427 (1996).

Endemann et al, "Novel anti-inflammatory compounds prevent CD 1 lb/Cd 18, alpha M beta 2 (mac- Independent neutrophil adhesion without blocking activation-induced changes in Mac-I," JPharm & Exp Therap. 276:5, 1996.

Gerszten et al, "Adhesion of memory lymphocytes to vascular cell adhesion molecule-1 transducted human vascular endothelial cells under simulated physiological flow conditions in vitro," Circulatory Research 79:1205, 1996.

Goligorsky et al, "Therapeutic effect of arginine-glycine-aspartic acid peptides in acute renal injury," Clin Exper Pharm Physiol 25:276, 1998.

Greenwood et al, "Lymphocyte adhesion and transendothelial. migration in the central nervous system: the role of LFA-1, ICAM-1, VLA-4, and VACM-I," Immunology 86:408, 1995.

Gundel et al, "The Role of Intercellular Adhesion Molecule-1 in Chronic Airway Inflammation," Clin Exp Allergy, 22:569, 1992.

Hamamoto et al, "Impact of adhesion molecules of the selectin family on liver microcirculation at reperfiision following cold ischemia," Transplant International 9:454, 1996.

H-irning et al, "Monoclonal antibodies to lymphocyte function-associated antigen- 1 inhibit invasion of human lymphoma and metastasis of murine lymphoma. Clin. Exp. Metastasis, 11:337, 1993. Hasegawa et al., Prevention of autoimmune insulin-dependent diabetes in non-obese mice by anti-LFA-1 and anti-ICAM-1 mAb," Int. Immunol, 6:831, 1994.

Huang et al, "Immunoglobulin superfamily protiens: structure, mechanisms, and drug discovery," Bioploymers 45:367, 1997.

Flaming et al, "Monoclonal antibodies to lymphocyte function-associated antigen- 1 inhibit invasion of human lymphoma and metastasis of murine lymphoma," Clin Exp Metastasis 11:337, 1993.

Isobe et al, "Specific acceptance of cardiac allograft after treatment with anti-ICAM-1 and anti-LFA-1," Science, 255:1125, 1992.

Jackson et al, "Potent alpha 4 beta 1 peptide antagonists as potential anti-inflammatory agents," J Medicinal Chem 40:3359,1997.

Jacobsson and Frykberg, "Phage display shot-gun cloning of ligand-binding domains of prokaryotic receptors approaches 100% correct clones," Biotechniques 20:1070, 1996.

Johnston et al, "On the species specificity of the interaction of LFA-1 with intercellular adhesion molecules," J Immunol 145:1181, 1990.

Jones et al, "A two-step adhesion cascade for T cell/endothelial cell interactions under flow conditions," JClin Invest, 94:2443, 1994.

Kavanaugh et al, "Treatment of refractory rheumatoid arthritis with a monoclonal antibody to intercellular adhesion molecule-1," Arthritis Rheum., 37:992, 1994.

Larson et al, "Retinoic acid induces aggregation of the acute promyelocytic leukemia cell line NB-4 that is mediated by LFA-1 and ICAM-2," Blood, 90:2747, 1997.

Larson et al, "Primary structure of the LFA-1 alpha subunit: An integrin with an embedded domain defining a protein superfamily," JCellBiol, 108:703, 1989.

Larson et al, "The subunit specificity of CDll a/18, CDllb, and CDllc panels of antibodies," in Leukocyte Typing, p 566, 1990.

Larson et al, "The leukocyte integrin LFA-1 reconstituted by cDNA transfection in a nonhematopoietic cell line is fimctionally active and not transiently regulated," Cell Regul, 1:359,1990.

Larson and McCurley, "CD4 Predicts Nonlymphocytic Lineage in Acute Leukemia: Insights From Analysis of 125 Cases Using Two-Color Flow Cytometry," Am J Clin Path, 104:204, 1995.

Larson and Springer, "The structure and function of leukocyte integrins," Immunol Rev 114:181, 1990. Larson et al, "All-trans retinoic acid induces neutrophil-like adhesion phenotype on acute promyeliocytic cells (abstract)," Blood 90(Suppl l):522a, 1997.

Lawrence et al, "Effect of venous shear stress on CD 18-mediated neutrophil adhesion to cultured endothelium," Blood 75:227, 1990.

Lefkovis and Topol, "Platelet glycoprotein Hb/Ifla receptor inhibitors in ischemic heart disease," Current Opinion Cardiology 10:420, 1995.

Liesveld et al, "Adhesive interactions of normal and leukemic human CD34+ myeloid progemtors: Role of marrow stromal, fibroblasts and cytomatrix components," Exp Hematol 19:63, 1991.

Luscinskas et al, "Endothehal-leukocyte adhesion molecule dependent and leukocyte (CD 11 CD 18) dependent mechanisms contribute to polymorphomiclear leukocyte adhesion to cytokine activated human vascular endothelium," J Immunol 142:2257, 1989.

Luscinskas et al, "L- and P-selectins, but not CD49d (VLA-4) integrins, mediate monocyte initial attachment to TNF-alpha activated vascular endothelium under flow in vivo," J Immunol, 157:326, 1996.

Ma et al, "In vivo treatment with anti-ICAM-1 and anti-LFA-1 antibodies inhibits contact sensitization-induced migration of epidermal Langerhans cells to regional lymph nodes," Cell Immunol, 15:389, 1994

Manabe et al, "Bone marrow-derived stromal cells prevent apoptotic cell death in B- lineage acute lymphoblastic leukemia," Blood, 79:2370, 1992.

Manabe et al, "Adhesion-dependent survival of normal and leukemic human B lymphoblasts on bone marrow stromal cells," Blood 83:758, 1994.

Marubayashi et al, "Protective effect of monoclonal antibodies to adhesion molecules on rat liver ischemia-reperfusion injury," Surgery, 122:45, 1997.

McEvei, "Leukocyte interaction mediated by selectins," Thrombos Hemost, 66:80, 1990.

McMurray, "Adhesion molecules in autoimmune disease," Seminars in Arthritis & Rheumatism 25:215, 1996.

Murayarna et al, "Novel peptide ligands for integrin alpha 6 beta 1 selected from a phage display library," J Biochemistry, 120:445, 1996.

Nakakura et al, 'Potent and effective prolongation by anti-LFA-1 monoclonal antibody monotherapy of non-primarily vascularized heart allograft survival in mice without T-cell depletion," Transplantation, 55:412, 1993. Nakano et al, "Efficacy of intraportal injection of anti-ICAM-1 monoclonal antibody against liver cell injury following warm ischemia in the rat," Am J Surgery, 170:65, 1995.

Noiri et al, "Cyclic RGD peptides ameliorate ischemic acute renal failure in rats," Kidney Intl., 46:1050, 1994.

Ohman et al, "Early clinical experience with integrelin, an inhibitor of the platelet glycoprotein Ilb/IIIa integrin receptor," Eur Heart J, 16:50, 1995.

Ojima et al, "Antithrombotic agents: from RGD to peptide mimetics," Bioorg Med Chem, 3:337, 1995.

Pasqualini et al, "A peptide isolated from phage display libraries is a structural and ftmctional m__mic of- RGD-bmd_ng site on integrins," JCe//Rto/, 13 0:1189, 1995.

Picker and Butcher, "Physiological and molecular mechanisms of lymphocyte homing," Annu Rev Immunol, 10:561, 1992.

Ries et al, "Tables and Graphs.", in SEER cancer statistics review, 1973-1991., National Cancer Institute. National Institutes of Health publication, p 94, 1 94.

Roecklein and Torak-Storb, "Functionally Distinct Human Marrow Stromal Cell Lines immortalized by Transduction with the Human Papilloma Virus E6/E7 Genes," Blood, 85:97, 1995.

Romer et al , "Lack of in vitro synergy of clinically used antibodies specific for ICAM- 1 and LFA-1," Transplant Immunol, 5:162, 1997.

Servitje et al, "Changes in T-cell phenotype and adhesion molecules expression in psoriatic lesions after lose-dose cyclosporin therapy," J Cutan. Pathol, 23:431, 1996.

Simon et al, "Neutrophil aggregation in beta 2-integrin and L-selectin-dependent in blood and isolated cells," J. Immunol, 149:2765, 1992.

Springer, "Traffic signals of lymphocyte recirculation and leukocyte emigration: the multistep paradigm," Cell, 76:301, 1994.

Staunton et al, "The arrangement of the immunoglobulin-like domains of ICAM-1 and the binding sites for LFA-1 and rhinovirus, " Cell, 61:243, 1990.

Stepkowski et al, "Blocking of heart allograft rejection by intercellular adhesion molecule- I antisense oilgonucelotides alone or in combination with other immunosuppressive modalities," J Immunol, 144:4604, 1990.

Suehiro et al, "The ligand recognition specificity of beta3 integrins," JBiol Chem, 271: 10365, 1996. Talento et al, "A single adminsitration of LFA-1 antibody confers prolonged allograft survival," Transplantation, 55:418, 1993.

Tallman et al, "AU-trans-retinoic acid in acute promyelocytic leukemia," NEng JMed, 337:1021, 1997.

Tibbets et al, "Peptides derived from ICAM-1 and LFA-1 modulate T cell adhesion and immune function in a mixed lymphocyte culture," Transplantation, 68:685, 1999.

Van der Vieren et al, "A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM-3," Immunity, 3:683, 1995.

Vanderslice et al, "A cyclic hexapeptide is a potent antagonist of alpha4 integrins," J Immunol, 158:1710, 1997.

Wang et al, "Epstein-Barr virus latent infection membrane (LMP) protein alters lymphocyte morphology, adhesion, and growth: Deletion of the amino terminus abolishes activity," J Virol 62:4173, 1988.

Winter et al, "Combinatorial effects of LFA-1 dependent stromal cell adherence and soluble factor(s) on T-ALL cell survival (abstract)," Blood, 89 (suppl l):81a, 1998.

Zhang and Plow, "A discrete site modulates activation of I domains. Application to integrin alpha Mbeta2," JBiol Chem, 271:29953, 1996.

Claims

WHAT IS CLAIMED IS:

1. A composition comprising a cyclic peptide inhibitor of LFA-l/ICAM-1 interaction, wherein said cyclic peptide inhibitor has 7-10 amino acids and comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55.

2. The composition of claim 1, wherein said composition is contained in a pharmaceutically acceptable carrier.

3. The composition of claim 1, wherein said cyclic peptide inhibitor of LFA- l/ICAM-1 interaction has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

4. The composition of claim 1, wherein said composition exhibits an IC₅₀ of cell aggregation mediated by LFA-l/ICAM-1 binding of from about 10 μM to about 850 μM.

5. The composition of claim 1, wherein said composition exhibits an IC₅₀ of monovalent ICAM-1 binding of from about 10 nM to about 250 nM.

6. A composition comprising a cyclic peptide inhibitor of LFA-l/ICAM-1 interaction, wherein said cyclic peptide inhibitor has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

7. The composition of claim 6, wherein said composition is contained in a pharmaceutically acceptable carrier.

8. The composition of claim 6, wherein said composition exhibits an IC₅₀ of cell aggregation mediated by LFA-l/ICAM-1 binding of from about 10 μM to about 850 μM.

9. The composition of claim 6, wherein said composition exhibits an IC₅₀ of monovalent ICAM-1 binding of from about 10 nM to about 250 nM.

10. A method of inhibiting in a subject the interaction between LFA-1 expressed on a leukocyte and ICAM-1 expressed on a second cell, comprising administering to said subject an effective amount of a composition comprising a cyclic peptide, wherein said cyclic peptide has 7-10 amino acids and comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55.

11. The method of claim 10, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

12. The method of claim 10, wherein said second cell is an bone marrow stromal cell.

13. The method of claim 10, wherein said second cell is an endothelial cell.

14. A method of preventing retinoic acid syndrome in a subject receiving all-trans retinoic acid, comprising administering to said subject an effective amount of a composition comprising a cyclic peptide, wherein said cyclic peptide has 7-10 amino acids and comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55.

15. The method of claim 14, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

16. A method for inhibiting growth of leukemia cells comprising preventing an LFA-1 /ICAM-1 interaction between said leukemia cells and bone marrow stromal cells, wherein said method comprises contacting said bone marrow stromal cells with a cyclic peptide inhibitor of said LFA-1/ICAMl interaction, wherein said cyclic peptide has 7-10 amino acids and comprises an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55.

17. The method of claim 16, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

18. A therapeutic package for dispensing to, or for use in dispensing to, a mammal being treated for a hematopoietic neoplastic disease, myocardial infarction, radiation injury, asthma, rheumatoid arthritis, or lymphoma metastasis, wherein said package contains in a unit dose, an amount of a cyclic peptide of 7-10 amino acids and comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55.

19. The method of claim 18, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

20. The package of claim 18, wherein said package is formulated for intravenous administration.

21. The package of claim 18, wherein said package includes said unit dose in a dry powder form.

22. The package of claim 18, wherein said unit dose will deliver a dose from about 10 μg/Kg to about 500 μg/Kg.

23. The package of claim 18, wherein said unit dose will deliver a dose from about 50 μg/Kg to about 250 μg/Kg.

24. A method of inhibiting emigration of leukocytes from blood into tissue in a subject comprising administering to said subject an amount of a cyclic peptide having 7- 10 amino acids and comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SE0 ID NO: 55, effective to inhibit an LFA-l/ICAM-1 interaction in said subject.

25. The method of claim 24, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

26. The method of claim 24, wherein said subject is susceptible to the development of or is suffering from a hematopoietic neoplastic disease, myocardial infarction, radiation injury, asthma, rheumatoid arthritis, or lymphoma metastasis.

27. A method of screening a candidate compound for binding to ICAM-1 comprising: (a) contacting ICAM-1 with a peptide having 7-10 amino acids and comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 49, SEQ ID NO: 50, SEQ ID NO: 51, SEQ ID NO: 52, SEQ ID NO: 53, SEQ ID NO: 54 and SEQ ID NO: 55, such that said peptide, is bound to the said ICAM-1 : (b) contacting said ICAM-1 with said bound peptide with said candidate compound;

(c) quantifying displaced peptide.

28. The method of claim 27, wherein said cyclic peptide has an amino acid sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 40, SEQ ID NO: 43, SEQ ID NO: 44, SEQ ID NO: 45, SEQ ID NO: 46 and SEQ ID NO: 48.

29. The method of claim 27, wherein said peptide is radiolabeled.

30. The method of claim 27, wherein said peptide is labeled with a flurophore.