WO2001047956A3 - Method for obtaining proteins having improved functional characteristics - Google Patents

Method for obtaining proteins having improved functional characteristics Download PDF

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Publication number
WO2001047956A3
WO2001047956A3 PCT/US2000/033878 US0033878W WO0147956A3 WO 2001047956 A3 WO2001047956 A3 WO 2001047956A3 US 0033878 W US0033878 W US 0033878W WO 0147956 A3 WO0147956 A3 WO 0147956A3
Authority
WO
WIPO (PCT)
Prior art keywords
amino acid
functional characteristics
protein
improved functional
improved
Prior art date
Application number
PCT/US2000/033878
Other languages
French (fr)
Other versions
WO2001047956A2 (en
Inventor
Anthony G Day
Colin Mitchinson
Andrew Shaw
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Priority to DK00984363.2T priority Critical patent/DK1240524T3/en
Priority to DE60045255T priority patent/DE60045255D1/en
Priority to AT00984363T priority patent/ATE488770T1/en
Priority to CA2394740A priority patent/CA2394740C/en
Priority to JP2001549426A priority patent/JP4658433B2/en
Priority to EP00984363A priority patent/EP1240524B1/en
Priority to AU20993/01A priority patent/AU2099301A/en
Publication of WO2001047956A2 publication Critical patent/WO2001047956A2/en
Publication of WO2001047956A3 publication Critical patent/WO2001047956A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

An improved protein comprising an amino acid sequence which has been modified from a precursor amino acid sequence by the substitution or deletion of an amino acid residue which differs from a corresponding amino acid residue in a less stable but homologous protein, wherein said improved protein has improved properties compared to a protein corresponding to the precursor amino acid sequence.
PCT/US2000/033878 1999-12-23 2000-12-14 Method for obtaining proteins having improved functional characteristics WO2001047956A2 (en)

Priority Applications (7)

Application Number Priority Date Filing Date Title
DK00984363.2T DK1240524T3 (en) 1999-12-23 2000-12-14 Cellulase from T. Reesei with improved thermostability
DE60045255T DE60045255D1 (en) 1999-12-23 2000-12-14 CELLULASE OF T. REESEI WITH IMPROVED THERMOSTABILITY
AT00984363T ATE488770T1 (en) 1999-12-23 2000-12-14 CELLULASE FROM T. REESEI WITH IMPROVED THERMOSTABILITY
CA2394740A CA2394740C (en) 1999-12-23 2000-12-14 Method for obtaining proteins having improved functional characteristics
JP2001549426A JP4658433B2 (en) 1999-12-23 2000-12-14 Method for obtaining a protein with improved functional properties
EP00984363A EP1240524B1 (en) 1999-12-23 2000-12-14 Cellulase from t. reesei with improved thermostability
AU20993/01A AU2099301A (en) 1999-12-23 2000-12-14 Method for obtaining proteins having improved functional characteristics

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US47083299A 1999-12-23 1999-12-23
US09/470,832 1999-12-23

Publications (2)

Publication Number Publication Date
WO2001047956A2 WO2001047956A2 (en) 2001-07-05
WO2001047956A3 true WO2001047956A3 (en) 2002-02-14

Family

ID=23869235

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/033878 WO2001047956A2 (en) 1999-12-23 2000-12-14 Method for obtaining proteins having improved functional characteristics

Country Status (10)

Country Link
EP (2) EP2184350B1 (en)
JP (1) JP4658433B2 (en)
AT (1) ATE488770T1 (en)
AU (1) AU2099301A (en)
CA (1) CA2394740C (en)
DE (1) DE60045255D1 (en)
DK (2) DK2184350T3 (en)
ES (1) ES2354866T3 (en)
PT (1) PT1240524E (en)
WO (1) WO2001047956A2 (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6407046B1 (en) 1998-09-03 2002-06-18 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6579841B1 (en) * 1998-12-18 2003-06-17 Genencor International, Inc. Variant EGIII-like cellulase compositions
US20020155574A1 (en) 2000-08-01 2002-10-24 Novozymes A/S Alpha-amylase mutants with altered properties
US6623949B1 (en) 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6635465B1 (en) * 2000-08-04 2003-10-21 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
CA2417857C (en) * 2000-08-04 2012-03-06 Genencor International, Inc. Mutant egiii cellulase, dna encoding such egiii compositions and methods for obtaining same
JP2003250544A (en) * 2002-03-04 2003-09-09 National Institute Of Technology & Evaluation Method for modifying property of protein
JP5427342B2 (en) * 2003-04-01 2014-02-26 ジェネンコー・インターナショナル・インク Mutant Humicola Grisea CBH1.1
WO2005111203A2 (en) * 2004-04-08 2005-11-24 Genencor International, Inc. MUTANT α ΑMYLASES
US7629158B2 (en) * 2006-06-16 2009-12-08 The Procter & Gamble Company Cleaning and/or treatment compositions
KR20100024395A (en) * 2007-05-30 2010-03-05 다니스코 유에스 인크. Improved variants of the bacillus licheniformis alpha-amylase
FI122028B (en) * 2008-12-30 2011-07-29 Ab Enzymes Oy Endoglucanases derived from fungi, their preparation and use
CA2771071C (en) * 2009-08-07 2020-03-10 Danisco Us Inc. Alpha-amylase blend for starch processing and method of use thereof
WO2018150260A1 (en) * 2017-02-16 2018-08-23 Institut Pasteur Method for the identification of mutated proteins having modified thermal stability
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994018314A1 (en) * 1993-02-11 1994-08-18 Genencor International, Inc. Oxidatively stable alpha-amylase
WO1995024471A1 (en) * 1994-03-08 1995-09-14 Novo Nordisk A/S Novel alkaline cellulases
WO1996023874A1 (en) * 1995-02-03 1996-08-08 Novo Nordisk A/S A method of designing alpha-amylase mutants with predetermined properties
WO1998026078A1 (en) * 1996-12-09 1998-06-18 Genencor International, Inc. H mutant alpha-amylase enzymes
WO1999029876A2 (en) * 1997-12-09 1999-06-17 Genencor International, Inc. Mutant bacillus licheniformis alpha-amylase

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5861271A (en) * 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
US5736499A (en) * 1995-06-06 1998-04-07 Genencor International, Inc. Mutant A-amylase
DE69836227T2 (en) * 1997-12-16 2007-08-23 Genencor International, Inc., Palo Alto PROCESS FOR THE PREPARATION OF EGIII-SIMILAR ENZYMES
US6187732B1 (en) * 1998-09-03 2001-02-13 Genencor International, Inc. Mutant EGIII cellulase, DNA encoding such EGIII compositions and methods for obtaining same
US6268328B1 (en) * 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
US6623949B1 (en) * 2000-08-04 2003-09-23 Genencor International, Inc. Variant EGIII-like cellulase compositions

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994018314A1 (en) * 1993-02-11 1994-08-18 Genencor International, Inc. Oxidatively stable alpha-amylase
WO1995024471A1 (en) * 1994-03-08 1995-09-14 Novo Nordisk A/S Novel alkaline cellulases
WO1996023874A1 (en) * 1995-02-03 1996-08-08 Novo Nordisk A/S A method of designing alpha-amylase mutants with predetermined properties
WO1998026078A1 (en) * 1996-12-09 1998-06-18 Genencor International, Inc. H mutant alpha-amylase enzymes
WO1999029876A2 (en) * 1997-12-09 1999-06-17 Genencor International, Inc. Mutant bacillus licheniformis alpha-amylase

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HOLM L ET AL: "RANDOM MUTAGENESIS USED TO PROBE THE STRUCTURE AND FUNCTION OF BACILLUS-STEAROTHERMOPHILUS ALPHA AMYLASE", PROTEIN ENGINEERING, vol. 3, no. 3, 1990, pages 181 - 192, XP000087332, ISSN: 0269-2139 *
NIELSEN JENS ERIK ET AL: "Protein engineering of bacterial alpha-amylases.", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1543, no. 2, 2000, pages 253 - 274, XP000984337, ISSN: 0006-3002 *
SUZUKI Y ET AL: "AMINO ACID RESIDUES STABILIZING A BACILLUS ALPHA AMYLASE AGAINST IRREVERSIBLE THERMOINACTIVATION", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 264, no. 32, 1989, pages 18933 - 18938, XP000872071, ISSN: 0021-9258 *
TOMAZIC S J ET AL: "WHY IS ONE BACILLUS ALPHA AMYLASE MORE RESISTANT AGAINST IRREVERSIBLE THERMOINACTIVATION THAN ANOTHER?", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 263, no. 7, 1988, pages 3092 - 3096, XP001015592, ISSN: 0021-9258 *

Also Published As

Publication number Publication date
DK1240524T3 (en) 2011-02-14
EP1240524A2 (en) 2002-09-18
JP4658433B2 (en) 2011-03-23
CA2394740C (en) 2011-02-01
EP2184350B1 (en) 2014-09-03
AU2099301A (en) 2001-07-09
ATE488770T1 (en) 2010-12-15
DE60045255D1 (en) 2010-12-30
EP2184350A2 (en) 2010-05-12
EP1240524B1 (en) 2010-11-17
EP2184350A3 (en) 2010-06-02
ES2354866T3 (en) 2011-03-18
CA2394740A1 (en) 2001-07-05
WO2001047956A2 (en) 2001-07-05
DK2184350T3 (en) 2014-11-24
JP2004500815A (en) 2004-01-15
PT1240524E (en) 2011-02-02

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