WO2001040455A3 - Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals - Google Patents

Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals Download PDF

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Publication number
WO2001040455A3
WO2001040455A3 PCT/US2000/032235 US0032235W WO0140455A3 WO 2001040455 A3 WO2001040455 A3 WO 2001040455A3 US 0032235 W US0032235 W US 0032235W WO 0140455 A3 WO0140455 A3 WO 0140455A3
Authority
WO
WIPO (PCT)
Prior art keywords
packaging
methods
yields
compositions
recombinant adenoviruses
Prior art date
Application number
PCT/US2000/032235
Other languages
French (fr)
Other versions
WO2001040455A2 (en
Inventor
Guangping Gao
James M Wilson
Original Assignee
Univ Pennsylvania
Guangping Gao
James M Wilson
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pennsylvania, Guangping Gao, James M Wilson filed Critical Univ Pennsylvania
Priority to EP00980747A priority Critical patent/EP1234048A2/en
Priority to AU17968/01A priority patent/AU1796801A/en
Priority to JP2001542523A priority patent/JP2003523180A/en
Priority to US10/169,544 priority patent/US6821512B1/en
Priority to CA002392537A priority patent/CA2392537A1/en
Publication of WO2001040455A2 publication Critical patent/WO2001040455A2/en
Publication of WO2001040455A3 publication Critical patent/WO2001040455A3/en
Priority to US10/996,341 priority patent/US20070134203A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

A recombinant adenoviral vector which has multiple adenovirus packaging domains is provided. This vector has advantages over conventional adenoviral vectors in packaging plasmid vectors into adenoviral capsids. Methods of making and using this vector are described.
PCT/US2000/032235 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals WO2001040455A2 (en)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP00980747A EP1234048A2 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
AU17968/01A AU1796801A (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
JP2001542523A JP2003523180A (en) 1999-12-03 2000-11-27 Compositions and methods for increasing the packaging and yield of recombinant adenovirus using multiple packaging signals
US10/169,544 US6821512B1 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yield of recombinant adenoviruses using multiple packaging signals
CA002392537A CA2392537A1 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals
US10/996,341 US20070134203A1 (en) 1999-12-03 2004-11-23 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US16902599P 1999-12-03 1999-12-03
US60/169,025 1999-12-03

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/996,341 Continuation US20070134203A1 (en) 1999-12-03 2004-11-23 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Publications (2)

Publication Number Publication Date
WO2001040455A2 WO2001040455A2 (en) 2001-06-07
WO2001040455A3 true WO2001040455A3 (en) 2002-01-17

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/032235 WO2001040455A2 (en) 1999-12-03 2000-11-27 Compositions and methods for increasing packaging and yields of recombinant adenoviruses using multiple packaging signals

Country Status (5)

Country Link
EP (1) EP1234048A2 (en)
JP (1) JP2003523180A (en)
AU (1) AU1796801A (en)
CA (1) CA2392537A1 (en)
WO (1) WO2001040455A2 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9567607B2 (en) 1998-11-05 2017-02-14 Trustees Of The University Of Pennsylvania Adeno-associated virus serotype I nucleic acid sequences, vectors and host cells containing same

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6806058B2 (en) 2001-05-26 2004-10-19 One Cell Systems, Inc. Secretions of proteins by encapsulated cells
US7217570B2 (en) 2001-08-23 2007-05-15 The Wistar Institute Of Anatomy And Biology Organotypic intestinal culture and methods of use thereof
DK1310571T3 (en) 2001-11-13 2006-06-19 Univ Pennsylvania Method for identifying unknown adeno-associated virus sequences (AAV sequences) and a kit for the method
EP1463805B1 (en) 2001-12-17 2014-10-22 The Trustees of The University of Pennsylvania Adeno-associated virus (aav) serotype 9 sequences, vectors containing same, and uses therefor
EP1453547B1 (en) 2001-12-17 2016-09-21 The Trustees Of The University Of Pennsylvania Adeno-associated virus (aav) serotype 8 sequences, vectors containing same, and uses therefor
LT3116900T (en) 2014-03-09 2020-12-10 The Trustees Of The University Of Pennsylvania Compositions useful in treatment of ornithine transcarbamylase (otc) deficiency

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999053085A2 (en) * 1998-04-15 1999-10-21 The Research Foundation Of State University Of New York Selective regulation of adenovirus production

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999053085A2 (en) * 1998-04-15 1999-10-21 The Research Foundation Of State University Of New York Selective regulation of adenovirus production

Non-Patent Citations (8)

* Cited by examiner, † Cited by third party
Title
GRABLE M ET AL: "CIS AND TRANS REQUIREMENTS FOR THE SELECTIVE PACKAGING OF ADENOVIRUS TYPE 5 DNA", JOURNAL OF VIROLOGY,US,NEW YORK, US, vol. 66, no. 2, 1 February 1992 (1992-02-01), pages 723 - 731, XP002012398, ISSN: 0022-538X *
HEHIR K M ET AL: "MOLECULAR CHARACTERIZATION OF REPLICATION-COMPETENT VARIANTS OF ADENOVIRUS VECTORS AND GENOME MODIFICATIONS TO PREVENT THEIR OCCURRENCE", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 70, no. 12, December 1996 (1996-12-01), pages 8459 - 8467, XP002911790, ISSN: 0022-538X *
KOCHANEK S ET AL: "A NEW ADENOVIRAL VECTOR: REPLACEMENT OF ALL VIRAL CODING SEQUENCES WITH 28 KB OF DNA INDEPENDENTLY EXPRESSING BOTH FULL-LENGTH DYSTROPHIN AND BETA-GALACTOSIDASE", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA,US,NATIONAL ACADEMY OF SCIENCE. WASHINGTON, vol. 93, 1 June 1996 (1996-06-01), pages 5731 - 5736, XP000652052, ISSN: 0027-8424 *
LANUTI, M. ET AL.: "Evaluation of an E1E4-delted adenovirus expressing the Herpes simplex thymidine kinase suicide gene in cancer gene therapy", HUMAN GENE THERAPY, vol. 10, 10 February 1999 (1999-02-10), pages 463 - 475, XP000995599 *
SANDIG, V. ET AL.: "Optimization of the helper-dependent adenovirus system for production and potency in vivo", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 97, no. 3, 1 February 2000 (2000-02-01), pages 1002 - 1007, XP000985990 *
SCHMID S I AND HEARING P: "Bipartite structure and functional independence of adenovirus 5 packaging elements", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 71, no. 5, May 1997 (1997-05-01), pages 3375 - 3384, XP002108134, ISSN: 0022-538X *
SCHMID S I ET AL: "Cellular components interact with adenovirus type 5 minimal DNA packaging domains", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 72, no. 8, August 1998 (1998-08-01), pages 6339 - 6347, XP002117828, ISSN: 0022-538X *
SEGGERN VON D J ET AL: "A HELPER-INDEPENDENT ADENOVIRUS VECTOR WITH E1, E3, AND FIBER DELETED: STRUCTURE AND INFECTIVITY OF FIBERLESS PARTICLES", JOURNAL OF VIROLOGY,US,THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 73, no. 2, 1999, pages 1601 - 1608, XP000906914, ISSN: 0022-538X *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9567607B2 (en) 1998-11-05 2017-02-14 Trustees Of The University Of Pennsylvania Adeno-associated virus serotype I nucleic acid sequences, vectors and host cells containing same

Also Published As

Publication number Publication date
JP2003523180A (en) 2003-08-05
AU1796801A (en) 2001-06-12
WO2001040455A2 (en) 2001-06-07
CA2392537A1 (en) 2001-06-07
EP1234048A2 (en) 2002-08-28

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