WO2001035725A1 - Yield-related genes - Google Patents
Yield-related genes Download PDFInfo
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- WO2001035725A1 WO2001035725A1 PCT/US2000/031414 US0031414W WO0135725A1 WO 2001035725 A1 WO2001035725 A1 WO 2001035725A1 US 0031414 W US0031414 W US 0031414W WO 0135725 A1 WO0135725 A1 WO 0135725A1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Definitions
- sucrose a disaccha ⁇ de sugar used for systemically transporting carbon and energy in most plants
- sucrose a disaccha ⁇ de sugar used for systemically transporting carbon and energy in most plants
- Manipulation of the sucrose signaling pathway in seeds may therefore cause seeds to have more protein, oil or carbohydrate, depending on the type of manipulation.
- sucrose is converted to starch which is used as an energy store.
- sugar signaling pathways may partially determine the levels of starch synthesized m the tubers (Zrenner et al (1996) Plant J. 9.671-681)
- the manipulation of sugar signaling in tubers could lead to tubers with a higher starch content.
- manipulating the sugar signal transduction pathway may lead to altered gene expression to produce plants with desirable traits.
- manipulation of sugar signal transduction pathways could be used to alter source-sink relationships in seeds, tubers, roots and other storage organs leading to an increase m yield.
- the present invention provides novel transcription factors useful for modifying a plant's phenotype in desirable ways by modifying a plant's sugar-sensing characteristics and thereby, increasing the yield
- the invention relates to a recombinant polynucleotide comprising a nucleotide sequence selected from the group consisting of.
- the invention in another aspect, relates to a method of identifying a factor that is modulated by or interacts with a polypeptide encoded by a polynucleotide of the invention.
- the method comprises expressing a polypeptide encoded by the polynucleotide in a plant; and identifying at least one factor that is modulated by or interacts with the polypeptide.
- the method for identifying modulating or interacting factors is by detecting binding by the polypeptide to a promoter sequence, or by detecting interactions between an additional protein and the polypeptide in a yeast two hybrid system, or by detecting expression of a factor by hybridization to a microarray, subtractive hybridization or differential display.
- the invention is a method of identifying a molecule that modulates activity or expression of a polynucleotide or polypeptide of interest.
- the method comprises placing the molecule in contact with a plant comprising the polynucleotide or polypeptide encoded by the polynucleotide of the invention and monito ⁇ ng one or more of the expression level of the polynucleotide in the plant, the expression level of the polypeptide in the plant, and modulation of an activity of the polypeptide in the plant.
- the invention relates to an integrated system, computer or computer readable medium comp ⁇ sing one or more character strings corresponding to a polynucleotide of the invention, or to a polypeptide encoded by the polynucleotide.
- the integrated system, computer or computer readable medium may comprise a link between one or more sequence st ⁇ ngs to a modified plant sugar-sensing trait.
- the method may further comprise of linking the one or more of the polynucleotides of the invention, or encoded polypeptides, to a modified plant sugar-sensing phenotype.
- Figure 1 provides a table of exemplary polynucleotide and polypeptide sequences of the invention.
- the table includes from left to right for each sequence: the SEQ ID No., the internal code reference number (GID), whether the sequence is a polynucleotide or polypeptide sequence, and identification of any conserved domains for the polypeptide sequences.
- GID internal code reference number
- Figure 3 provides a table of exemplary sequences that are homologous to the sequences provided in Figures 1 and 2 and that are derived from plants other than Arabidopsis thaliana.
- the table includes from left to right: the SEQ ID No., the internal code reference number (GID), the unique GenBank sequence ID No. (NID), the probability that the comparison was generated by chance (P-value), and the species from which the homologous gene was identified.
- GID internal code reference number
- NID unique GenBank sequence ID No.
- P-value the probability that the comparison was generated by chance
- the present invention relates to polynucleotides and polypeptides, e.g. for modifying phenotypes of plants.
- a "polynucleotide” is a nucleic acid sequence comprising a plurality of polymerized nucleotide residues, e.g., at least about 15 consecutive polymerized nucleotide residues, optionally at least about 30 consecutive nucleotides, at least about 50 consecutive nucleotides.
- a polynucleotide comprises a nucleotide sequence encoding a polypeptide (or protein) or a domain or fragment thereof.
- a "recombinant polynucleotide” is a polynucleotide that is not in its native state, e.g., the polynucleotide comprises a nucleotide sequence not found in nature, or the polynucleotide is in a context other than that in which it is naturally found, e.g., separated from nucleotide sequences with which it typically is in proximity in nature, or adjacent (or contiguous with) nucleotide sequences with which it typically is not in proximity.
- the sequence at issue can be cloned into a vector, or otherwise recombined with one or more additional nucleic acid.
- epitopic expression or altered expression further may relate to altered activity levels resulting from the interactions of the polypeptides with exogenous or endogenous modulators or from interactions with factors or as a result of the chemical modification of the polypeptides.
- fragment refers to a subsequence of the polypeptide.
- the fragment or domain is a subsequence of the polypeptide which performs at least one biological function of the intact polypeptide in substantially the same manner, or to a similar extent, as does the intact polypeptide.
- a polypeptide fragment can comprise a recognizable structural motif or functional domain such as a DNA binding domain that binds to a DNA promoter region, an activation domain or a domain for protein-protein interactions.
- phenotype that can be modified relate to the production of plant metabolites, such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds), or modified sugar (insoluble or soluble) and/or starch composition.
- plant metabolites such as variations in the production of taxol, tocopherol, tocotrienol, sterols, phytosterols, vitamins, wax monomers, anti-oxidants, amino acids, lignins, cellulose, tannins, prenyllipids (such as chlorophylls and carotenoids), glucosinolates, and terpenoids, enhanced or compositionally altered protein or oil production (especially in seeds
- Physical plant characteristics that can be modified include cell development (such as the number of trichomes), fruit and seed size and number, yields of plant parts such as stems, leaves and roots, the stability of the seeds during storage, characteristics of the seed pod (e.g., susceptibility to shattering), root hair length and quantity, internode distances, or the quality of seed coat.
- Plant growth characteristics that can be modified include growth rate, germination rate of seeds, vigor of plants and seedlings, leaf and flower senescence, male sterility, apomixis, flowering time, flower abscission, rate of nitrogen uptake, biomass or transpiration characteristics, as well as plant architecture characteristics such as apical dominance, branching patterns, number of organs, organ identity, organ shape or size.
- the present invention provides, among other things, transcription factors (TFs), and transcription factor homologue polypeptides, and isolated or recombinant polynucleotides encoding the polypeptides. These polypeptides and polynucleotides may be employed to modify a plant's sugar-sensing characteristics.
- Stnngency is influenced by a variety of factors, including temperature, salt concentration and composition, organic and non-organic additives, solvents, etc. present in both the hyb ⁇ dization and wash solutions and incubations (and number), as desc ⁇ bed in more detail in the references cited above.
- substitutions, deletions and insertions introduced into the sequences provided in the Sequence Listing are also envisioned by the invention.
- sequence modifications can be engineered into a sequence by site-directed mutagenesis (Wu (ed.) Meth. Enzymol (1993) vol. 217, Academic Press) or the other methods noted below.
- Amino acid substitutions are typically of single residues; insertions usually will be on the order of about from 1 to 10 amino acid residues; and deletions will range about from 1 to 30 residues.
- deletions or insertions are made in adjacent pairs, e.g., a deletion of two residues or insertion of two residues. Substitutions, deletions, insertions or any combination thereof can be combined to arrive at a sequence.
- Conservative substitutions are those in which at least one residue in the amino acid sequence has been removed and a different residue inserted in its place. Such substitutions generally are made in accordance with the Table 2 when it is desired to maintain the activity of the protein. Table 2 shows amino acids which can be substituted for an amino acid in a protein and which are typically regarded as conservative substitutions.
- sequence can be modified by addition of lipids, sugars, peptides, organic or inorganic compounds, by the inclusion of modified nucleotides or ammo acids, or the like.
- protein modification techniques are illustrated in Ausubel, supra. Further details on chemical and enzymatic modifications can be found herein. These modification methods can be used to modify any given sequence, or to modify any sequence produced by the various mutation and artificial evolution modification methods noted herein.
- the invention provides for modification of any given nucleic acid by mutation, evolution, chemical or enzymatic modification, or other available methods, as well as for the products produced by practicing such methods, e.g., using the sequences herein as a starting substrate for the vanous modification approaches.
- non-Ti vectors can be used to transfer the DNA into monocotyledonous plants and cells by using free DNA delivery techniques.
- free DNA delivery techniques can involve, for example, the use of liposomes, electroporation, microprojectile bombardment, silicon carbide whiskers, and viruses.
- polynucleotides also referred to herein as oligonucleotides, typically having at least 12 bases, preferably at least 15, more preferably at least 20, 30, or 50 bases, which hybridize under at least highly stringent (or ultra-high stringent or ultra-ultra- high stringent conditions) conditions to a polynucleotide sequence described above.
- the polynucleotides may be used as probes, primers, sense and antisense agents, and the like, according to methods as noted supra.
- Subsequences of the polynucleotides of the invention, including polynucleotide fragments and oligonucleotides are useful as nucleic acid probes and primers.
- Primer pairs can be used for amplification of a nucleic acid sequence, e.g., by the polymerase chain reaction (PCR) or other nucleic-acid amplification methods. See Sambrook and Ausubel, supra.
- the invention includes an isolated or recombinant polypeptide including a subsequence of at least about 15 contiguous amino acids encoded by the recombinant or isolated polynucleotides of the invention.
- such polypeptides, or domains or fragments thereof can be used as immunogens, e.g., to produce antibodies specific for the polypeptide sequence, or as probes for detecting a sequence of interest.
- a subsequence can range in size from about 15 amino acids in length up to and including the full length of the polypeptide.
- Another method for abolishing the expression of a gene is by insertion mutagenesis using the T-DNA of Agrobacterium tumefaciens. After generating the insertion mutants, the mutants can be screened to identify those containing the insertion in a transcnption factor or transc ⁇ ption factor homologue gene. Plants containing a single transgene insertion event at the desired gene can be crossed to generate homozygous plants for the mutation (Koncz et al. (1992) Methods in Arabidopsis Research, World Scientific).
- a plant trait can also be modified by using the cre-lox system (for example, as described in US Pat. No. 5,658,772).
- a plant genome can be modified to include first and second lox sites that are then contacted with a Cre recombinase. If the lox sites are in the same orientation, the intervening DNA sequence between the two sites is excised. If the lox sites are in the opposite onentation, the intervening sequence is inverted.
- BLAST algorithm One example algorithm that is suitable for determining percent sequence identity and sequence similanty is the BLAST algorithm, which is described in Altschul et al. J. Mol. Biol 215:403-410 (1990).
- Software for performing BLAST analyses is publicly available, e.g., through the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov/).
- HSPs high scoring sequence pairs
- T is referred to as the neighborhood word score threshold (Altschul et al., supra).
- the integrated system, or computer typically includes a user input interface allowing a user to selectively view one or more sequence records corresponding to the one or more character strings, as well as an instruction set which aligns the one or more character strings with each other or with an additional character st ⁇ ng to identify one or more region of sequence similanty.
- the system may include a link of one or more character strings with a particular phenotype or gene function.
- the system includes a user readable output element which displays an alignment produced by the alignment instruction set.
- Agrobacterium strain ABI was grown in 250 ml LB medium (Sigma) overnight at 28°C with shaking until an absorbance (A 600 ) of 0.5 - 1.0 was reached. Cells were harvested by centrifugation at 4,000 x g for 15 min at 4° C. Cells were then resuspended in 250 ⁇ l chilled buffer (1 mM HEPES, pH adjusted to 7.0 with KOH). Cells were centrifuged again as described above and resuspended in 125 ⁇ l chilled buffer.
- cells were immediately resuspended in 1.0 ml LB and allowed to recover without antibiotic selection for 2 - 4 hours at 28° C in a shaking incubator. After recovery, cells were plated onto selective medium of LB broth containing 100 ⁇ g/ml spectinomycin (Sigma) and incubated for 24-48 hours at 28° C. Single colonies were then picked and inoculated in fresh medium. The presence of the plasmid construct was verified by PCR amplification and sequence analysis.
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
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MXPA02004880A MXPA02004880A (en) | 1999-11-17 | 2000-11-14 | Yield related genes. |
EP00980406A EP1229780A4 (en) | 1999-11-17 | 2000-11-14 | Yield-related genes |
AU17671/01A AU1767101A (en) | 1999-11-17 | 2000-11-14 | Yield-related genes |
CA002390594A CA2390594A1 (en) | 1999-11-17 | 2000-11-14 | Yield-related genes |
BR0015635-3A BR0015635A (en) | 1999-11-17 | 2000-11-14 | Plant, polynucleotide, vector, cell, composition, polypeptide, methods for producing a plant and for identifying a factor that is modulated by, or interacts with, a coded polypeptide, a molecule that modulates the activity or expression of a polynucleotide or polypeptide of interest and a sequence similar or homologous to one or more polynucleotides and integrated system, half computerized or legible in computer |
Applications Claiming Priority (6)
Application Number | Priority Date | Filing Date | Title |
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US16622899P | 1999-11-17 | 1999-11-17 | |
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PCT/US2000/031325 WO2001036444A1 (en) | 1999-11-17 | 2000-11-14 | Plant developmental genes |
PCT/US2000/031458 WO2001036598A1 (en) | 1999-11-17 | 2000-11-14 | Environmental stress tolerance genes |
PCT/US2000/031418 WO2001035726A1 (en) | 1999-11-17 | 2000-11-14 | Pathogen tolerance genes |
PCT/US2000/031344 WO2001036597A1 (en) | 1999-11-17 | 2000-11-14 | Plant biochemistry-related genes |
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PCT/US2000/031344 WO2001036597A1 (en) | 1999-11-17 | 2000-11-14 | Plant biochemistry-related genes |
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AT (2) | ATE531804T1 (en) |
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---|---|---|---|---|
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WO2004050881A2 (en) * | 2002-12-02 | 2004-06-17 | Plant Bioscience Limited | A plant transcription factor that is involved in sugar signalling |
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WO2005021765A2 (en) * | 2003-08-27 | 2005-03-10 | Orf Liftaekni Hf. | Enhancing accumulation of heterologous polypeptides in plant seeds through targeted suppression of endogenous storage proteins |
WO2005038034A2 (en) * | 2003-10-14 | 2005-04-28 | Mendel Biotechnology, Inc. | Plant transcriptional regulators of abiotic stress |
EP1534843A2 (en) * | 2002-08-02 | 2005-06-01 | BASF Plant Science GmbH | Sugar and lipid metabolism regulators in plants iv |
WO2005085453A3 (en) * | 2004-03-05 | 2006-04-20 | Univ Bath | Seeds |
US7329797B2 (en) | 2004-12-08 | 2008-02-12 | Ceres, Inc. | Modulating plant carbon levels |
US7335510B2 (en) | 2004-12-16 | 2008-02-26 | Ceres, Inc. | Modulating plant nitrogen levels |
US7335760B2 (en) | 2004-12-22 | 2008-02-26 | Ceres, Inc. | Nucleic acid sequences encoding zinc finger proteins |
US7361749B2 (en) | 1999-02-25 | 2008-04-22 | Ceres, Inc. | Sequence-determined DNA encoding methyltransferases |
US7385046B2 (en) | 2001-01-03 | 2008-06-10 | Ceres, Inc. | Sequence-determined DNA fragments encoding ethylene responsive element binding proteins |
US7399850B2 (en) | 1999-06-18 | 2008-07-15 | Ceres, Inc. | Sequence-determined DNA fragments encoding AP2 domain proteins |
US7420049B2 (en) | 1999-06-18 | 2008-09-02 | Ceres, Inc. | Sequence-determined DNA fragments encoding AP2 domain proteins |
US7429692B2 (en) | 2004-10-14 | 2008-09-30 | Ceres, Inc. | Sucrose synthase 3 promoter from rice and uses thereof |
US7479555B2 (en) | 1999-07-21 | 2009-01-20 | Ceres, Inc. | Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity |
US7485715B2 (en) | 1999-06-18 | 2009-02-03 | Ceres, Inc. | Sequence-determined DNA encoding AP2 domain polypeptides |
US7595433B2 (en) | 2004-09-14 | 2009-09-29 | Ceres, Inc. | Modulations of amino acid and sugar content in plants |
US7691991B2 (en) | 2000-04-17 | 2010-04-06 | Ceres, Inc. | Sequence-determined DNA fragments encoding cytochrome P450 proteins |
WO2010147825A1 (en) | 2009-06-09 | 2010-12-23 | Pioneer Hi-Bred International, Inc. | Early endosperm promoter and methods of use |
US7897843B2 (en) | 1999-03-23 | 2011-03-01 | Mendel Biotechnology, Inc. | Transcriptional regulation of plant biomass and abiotic stress tolerance |
WO2011036569A2 (en) | 2009-09-22 | 2011-03-31 | The Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar cl 142-ar |
WO2011047191A1 (en) | 2009-10-15 | 2011-04-21 | The Board Of Trustees Of The University Of Arkansas, N.A. | Rice cultivar cl181-ar |
WO2011056544A1 (en) | 2009-10-26 | 2011-05-12 | Pioneer Hi-Bred International, Inc. | Somatic ovule specific promoter and methods of use |
US7964774B2 (en) | 2008-05-14 | 2011-06-21 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV384196 |
US8076535B2 (en) | 2004-09-14 | 2011-12-13 | Ceres, Inc. | Modulating plant sugar levels |
US8222482B2 (en) | 2006-01-26 | 2012-07-17 | Ceres, Inc. | Modulating plant oil levels |
EP2395093A3 (en) * | 2006-12-21 | 2012-08-01 | BASF Plant Science GmbH | Plants having enhanced yield-related traits and a method for making the same |
US8283519B2 (en) | 1998-09-22 | 2012-10-09 | Mendel Biotechnology, Inc. | Plant transcriptional regulators of abiotic stress |
WO2013096810A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11482 |
WO2013096818A1 (en) | 2011-12-21 | 2013-06-27 | The Curators Of The University Of Missouri | Soybean variety s05-11268 |
WO2013103365A1 (en) | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Pollen preferred promoters and methods of use |
WO2013103371A1 (en) | 2012-01-06 | 2013-07-11 | Pioneer Hi-Bred International, Inc. | Ovule specific promoter and methods of use |
WO2014059155A1 (en) | 2012-10-11 | 2014-04-17 | Pioneer Hi-Bred International, Inc. | Guard cell promoters and uses thereof |
US8835720B2 (en) | 2012-04-26 | 2014-09-16 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV967592 |
WO2014150914A2 (en) | 2013-03-15 | 2014-09-25 | Pioneer Hi-Bred International, Inc. | Phi-4 polypeptides and methods for their use |
WO2014153254A2 (en) | 2013-03-14 | 2014-09-25 | Pioneer Hi-Bred International Inc. | Compositions and methods to control insect pests |
WO2014159306A1 (en) | 2013-03-13 | 2014-10-02 | Pioneer Hi-Bred International, Inc. | Glyphosate application for weed control in brassica |
US8859857B2 (en) | 2012-04-26 | 2014-10-14 | Monsanto Technology Llc | Plants and seeds of spring canola variety SCV259778 |
US8878009B2 (en) | 2012-04-26 | 2014-11-04 | Monsanto Technology, LLP | Plants and seeds of spring canola variety SCV318181 |
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Families Citing this family (56)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7345217B2 (en) | 1998-09-22 | 2008-03-18 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
US20140196162A1 (en) | 2006-05-15 | 2014-07-10 | Mendel Biotechnology, Inc. | Plant transcriptional regulators |
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US7692067B2 (en) | 2002-09-18 | 2010-04-06 | Mendel Biotechnology, Inc. | Yield and stress tolerance in transgenic plants |
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US20060162018A1 (en) * | 2003-06-06 | 2006-07-20 | Gutterson Neil I | Plant transcriptional regulators of disease resistance |
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US7663025B2 (en) | 1999-03-23 | 2010-02-16 | Mendel Biotechnology, Inc. | Plant Transcriptional Regulators |
US20050086718A1 (en) | 1999-03-23 | 2005-04-21 | Mendel Biotechnology, Inc. | Plant transcriptional regulators of abiotic stress |
US7598429B2 (en) | 2001-04-18 | 2009-10-06 | Mendel Biotechnology, Inc. | Transcription factor sequences for conferring advantageous properties to plants |
US8686226B2 (en) | 1999-03-23 | 2014-04-01 | Mendel Biotechnology, Inc. | MYB-related transcriptional regulators that confer altered root hare, trichome morphology, and increased tolerance to abiotic stress in plants |
US8633353B2 (en) | 1999-03-23 | 2014-01-21 | Mendel Biotechnology, Inc. | Plants with improved water deficit and cold tolerance |
US9447425B2 (en) | 2000-11-16 | 2016-09-20 | Mendel Biotechnology, Inc. | Transcription factor sequences for conferring advantageous properties to plants |
US9322031B2 (en) | 1999-05-06 | 2016-04-26 | Monsanto Technology Llc | Transgenic plants with enhanced agronomic traits |
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US7067722B2 (en) | 1999-08-26 | 2006-06-27 | Monsanto Technology Llc | Nucleic acid sequences and methods of use for the production of plants with modified polyunsaturated fatty acids |
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US7939715B2 (en) | 2000-11-16 | 2011-05-10 | Mendel Biotechnology, Inc. | Plants with improved yield and stress tolerance |
DE10063986A1 (en) * | 2000-12-21 | 2002-06-27 | Max Planck Gesellschaft | Plants with improved resistance |
AU2002313749A1 (en) * | 2001-08-09 | 2003-02-24 | Mendel Biotechnology, Inc. | Biochemistry-related polynucleotides and polypeptides in plants |
US8426678B2 (en) * | 2002-09-18 | 2013-04-23 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
US7166771B2 (en) | 2002-06-21 | 2007-01-23 | Monsanto Technology Llc | Coordinated decrease and increase of gene expression of more than one gene using transgenic constructs |
US7566813B2 (en) | 2002-03-21 | 2009-07-28 | Monsanto Technology, L.L.C. | Nucleic acid constructs and methods for producing altered seed oil compositions |
CA2479587A1 (en) | 2002-03-21 | 2003-10-02 | Monsanto Technology Llc | Nucleic acid constructs and methods for producing altered seed oil compositions |
US20090183270A1 (en) | 2002-10-02 | 2009-07-16 | Adams Thomas R | Transgenic plants with enhanced agronomic traits |
EP1551983A2 (en) | 2002-10-18 | 2005-07-13 | CropDesign N.V. | Identification of e2f target genes and uses thereof |
US7663021B2 (en) | 2002-12-06 | 2010-02-16 | Del Monte Fresh Produce Company | Transgenic pineapple plants with modified carotenoid levels and methods of their production |
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CN101928712A (en) * | 2002-12-24 | 2010-12-29 | 作物培植股份有限公司 | Plant and production method thereof with growth characteristics of change |
EP1447410A1 (en) * | 2003-02-06 | 2004-08-18 | Universiteit Leiden | Control of plant growth and developmental processes |
US7888557B2 (en) | 2003-02-22 | 2011-02-15 | Monsanto Technology Llc | Transgenic plants |
KR20060119699A (en) * | 2003-10-06 | 2006-11-24 | 아리조나 보드 오브 리전츠 온 비해프 오브 유니버시티 오브 아리조나 | Snow1: interacts with ice1 and regulates cbf expression and freezing tolerance in arabidopsis |
AU2004290050A1 (en) * | 2003-11-13 | 2005-05-26 | Mendel Biotechnology, Inc. | Plant transcriptional regulators |
US9476039B2 (en) | 2003-12-05 | 2016-10-25 | Mendel Biotechnology, Inc. | Polynucleotides and polypeptides in plants |
AU2005253642B8 (en) * | 2004-06-15 | 2012-01-19 | Agriculture Victoria Services Pty Ltd | Nucleic acid molecules and their use in plant male sterility |
EP2241624A3 (en) | 2004-06-15 | 2011-02-09 | Latrobe University | Nucleic acid molecules and their use in plant sterility |
WO2006041280A1 (en) * | 2004-09-02 | 2006-04-20 | Universiteit Van Amsterdam | Novel regulation protein |
CN101421406B (en) | 2006-02-13 | 2016-08-31 | 孟山都技术有限公司 | For producing nucleic acid construct and the method that the seed oil of change forms |
US9080181B2 (en) | 2006-12-20 | 2015-07-14 | Fibria Celulose S.A. | Nucleic acid constructs methods for altering plant fiber length and/or plant height |
CA2708321C (en) | 2007-12-05 | 2014-11-18 | Toyota Jidosha Kabushiki Kaisha | Genes that increase plant oil and method for using the same |
EP2666864B1 (en) | 2007-12-05 | 2017-07-12 | Toyota Jidosha Kabushiki Kaisha | Genes that increase plant oil and method for using the same |
AU2008344053B2 (en) | 2007-12-28 | 2014-03-27 | Swetree Technologies Ab | Woody plants having improved growth charateristics and method for making the same using transcription factors |
JP5299886B2 (en) | 2008-03-04 | 2013-09-25 | トヨタ自動車株式会社 | Genes for increasing production of plant oils and methods of use thereof |
US20110209244A1 (en) * | 2008-09-29 | 2011-08-25 | National Institute Of Advanced Industrial Science And Technology | Method for production of plant imparted with stress tolerance and use thereof |
JP5767585B2 (en) | 2008-09-29 | 2015-08-19 | モンサント テクノロジー エルエルシー | Soybean genetic recombination event MON87705 and detection method thereof |
AU2010209875A1 (en) * | 2009-01-28 | 2011-08-18 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and a method for making the same |
CA2754108A1 (en) | 2009-03-05 | 2010-09-10 | Metabolix, Inc. | Propagation of transgenic plants |
JP5847991B2 (en) | 2009-06-04 | 2016-01-27 | トヨタ自動車株式会社 | Gene for improving substance productivity in seed and method for using the same |
JP5718554B2 (en) | 2009-06-04 | 2015-05-13 | トヨタ自動車株式会社 | Gene for increasing plant weight of plant and method for using the same |
JP5519192B2 (en) | 2009-06-04 | 2014-06-11 | トヨタ自動車株式会社 | Gene for increasing protein content of seed and method for using the same |
EP3184538A3 (en) * | 2010-03-04 | 2017-08-16 | Mendel Biotechnology, Inc. | Transcription regulators for improving plant performance |
US10087462B2 (en) * | 2015-01-06 | 2018-10-02 | Iowa State University Research Foundation, Inc. | Arabidopsis nonhost resistance gene(s) and use thereof to engineer SDS resistant plants |
Family Cites Families (72)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
WO1985001856A1 (en) | 1983-11-03 | 1985-05-09 | Johannes Martenis Jacob De Wet | Method for the transfer of exogenous genes in plants using pollen as a vector |
US4943674A (en) | 1987-05-26 | 1990-07-24 | Calgene, Inc. | Fruit specific transcriptional factors |
US5506337A (en) | 1985-03-15 | 1996-04-09 | Antivirals Inc. | Morpholino-subunit combinatorial library and method |
US5750871A (en) | 1986-05-29 | 1998-05-12 | Calgene, Inc. | Transformation and foreign gene expression in Brassica species |
US5597945A (en) | 1986-07-25 | 1997-01-28 | Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College | Plants genetically enhanced for disease resistance |
US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
US5543508A (en) | 1987-12-15 | 1996-08-06 | Gene Shears Pty. Limited | Ribozymes |
US5010175A (en) | 1988-05-02 | 1991-04-23 | The Regents Of The University Of California | General method for producing and selecting peptides with specific properties |
US5268526A (en) | 1988-07-29 | 1993-12-07 | E. I. Du Pont De Nemours And Company | Overexpression of phytochrome in transgenic plants |
US5589615A (en) | 1988-10-14 | 1996-12-31 | Plant Genetic Systems N.V. | Process for the production of transgenic plants with increased nutritional value via the expression of modified 2S storage albumins |
US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
US5689041A (en) | 1989-08-10 | 1997-11-18 | Plant Gentic Systems N.V. | Plants modified with barstar for fertility restoration |
US5658772A (en) | 1989-12-22 | 1997-08-19 | E. I. Du Pont De Nemours And Company | Site-specific recombination of DNA in plant cells |
US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
HU220773B1 (en) | 1990-01-22 | 2002-05-28 | Dekalb Genetics Corporation | Process for producing productive transgenic maize plants |
US5618988A (en) | 1990-03-02 | 1997-04-08 | Amoco Corporation | Enhanced carotenoid accumulation in storage organs of genetically engineered plants |
US5837848A (en) | 1990-03-16 | 1998-11-17 | Zeneca Limited | Root-specific promoter |
FR2673643B1 (en) | 1991-03-05 | 1993-05-21 | Rhone Poulenc Agrochimie | TRANSIT PEPTIDE FOR THE INSERTION OF A FOREIGN GENE INTO A PLANT GENE AND PLANTS TRANSFORMED USING THIS PEPTIDE. |
IL101508A0 (en) | 1991-04-08 | 1992-12-30 | Rhone Poulenc Agrochimie | Chimeric plant genes based on upstream regulatory elements of helianthinin |
DE69233410D1 (en) | 1991-10-04 | 2004-10-21 | Univ North Carolina State | PATHOGENRESISTENT TRANSGENIC PLANTS |
US5610042A (en) | 1991-10-07 | 1997-03-11 | Ciba-Geigy Corporation | Methods for stable transformation of wheat |
DE69331050T2 (en) | 1992-02-19 | 2002-06-20 | Oregon State | PRODUCTION OF VIRUS-RESISTANT PLANTS THROUGH THE INTRODUCTION OF NON-TRANSLATABLE VIRAL PLUS-STRAND RNA |
US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
US5519134A (en) | 1994-01-11 | 1996-05-21 | Isis Pharmaceuticals, Inc. | Pyrrolidine-containing monomers and oligomers |
US5593853A (en) | 1994-02-09 | 1997-01-14 | Martek Corporation | Generation and screening of synthetic drug libraries |
US5539083A (en) | 1994-02-23 | 1996-07-23 | Isis Pharmaceuticals, Inc. | Peptide nucleic acid combinatorial libraries and improved methods of synthesis |
US5571706A (en) | 1994-06-17 | 1996-11-05 | The United States Of America As Represented By The Secretary Of Agriculture | Plant virus resistance gene and methods |
US5525735A (en) | 1994-06-22 | 1996-06-11 | Affymax Technologies Nv | Methods for synthesizing diverse collections of pyrrolidine compounds |
US5549974A (en) | 1994-06-23 | 1996-08-27 | Affymax Technologies Nv | Methods for the solid phase synthesis of thiazolidinones, metathiazanones, and derivatives thereof |
US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
AU698152B2 (en) | 1994-08-20 | 1998-10-22 | Gendaq Limited | Improvements in or relating to binding proteins for recognition of DNA |
DE19503359C1 (en) * | 1995-02-02 | 1996-02-22 | Kws Kleinwanzlebener Saatzucht | Plants with increased tolerance towards stress |
US5569588A (en) | 1995-08-09 | 1996-10-29 | The Regents Of The University Of California | Methods for drug screening |
US5677175A (en) | 1995-10-13 | 1997-10-14 | Purdue Research Foundation | Plant pathogen induced proteins |
EP0776973A1 (en) * | 1995-12-08 | 1997-06-04 | Centrum Voor Plantenveredelings- En Reproduktieonderzoek | Fatty aldehyde decarbonylase for transforming bacterial cells and plants |
CA2213991C (en) * | 1995-12-27 | 2005-09-13 | Japan Tobacco Inc. | Cold-inducible promoter sequence |
US5783393A (en) | 1996-01-29 | 1998-07-21 | Agritope, Inc. | Plant tissue/stage specific promoters for regulated expression of transgenes in plants |
US6632602B1 (en) * | 1996-03-25 | 2003-10-14 | The General Hospital Corporation | Plant sugar sensors and uses thereof |
US5773697A (en) | 1996-04-23 | 1998-06-30 | Pioneer Hi-Bred International, Inc. | Genetic constructs and methods for producing fruits with very little or diminished seed |
JP4051719B2 (en) * | 1996-04-26 | 2008-02-27 | 東洋紡績株式会社 | CPC gene that controls initiation of root hair formation in Arabidopsis thaliana and plants incorporating the same |
MX205414B (en) * | 1996-05-08 | 2001-12-07 | Univ Mexico Nacional Autonoma | METHOD OF INCREASING TREHALOSE IN ORGANISMS BY TRANSFORMATION OF SELAGINELLA LEPIDOPHYLIA TREHALOSE-6-PHOSPHATE SINTASE/PHOSPHATASE cDNA. |
CA2258127A1 (en) * | 1996-06-12 | 1997-12-18 | Purdue Research Foundation | Genes enhancing disease resistance in plants |
US5773269A (en) | 1996-07-26 | 1998-06-30 | Regents Of The University Of Minnesota | Fertile transgenic oat plants |
US6329567B1 (en) * | 1996-08-20 | 2001-12-11 | The Regents Of The University Of California | Methods for improving seeds |
US6846669B1 (en) * | 1996-08-20 | 2005-01-25 | The Regents Of The University Of California | Methods for improving seeds |
US5892009A (en) * | 1996-09-04 | 1999-04-06 | Michigan State University | DNA and encoded protein which regulates cold and dehydration regulated genes |
US5939601A (en) * | 1996-09-27 | 1999-08-17 | Rutgers, The State University Of New Jersey | Genes associates with enhanced disease resistance in plants |
EP0977836A4 (en) * | 1997-02-21 | 2002-10-16 | Univ California | Leafy cotyledon1 genes and their uses |
AU6679398A (en) * | 1997-02-28 | 1998-09-18 | University Of North Carolina At Chapel Hill, The | Plant pathogen response gene |
US6388172B1 (en) * | 1997-04-18 | 2002-05-14 | The Regents Of The University Of California | Phytochrome regulated transcription factor for control of higher plant development |
GB9710809D0 (en) | 1997-05-23 | 1997-07-23 | Medical Res Council | Nucleic acid binding proteins |
WO1998058069A1 (en) * | 1997-06-17 | 1998-12-23 | Monsanto Company | Expression of fructose 1,6 bisphosphate aldolase in transgenic plants |
US6605704B1 (en) * | 1997-09-18 | 2003-08-12 | Japan Tobacco Inc. | Plant-origin regulator protein and nucleic acid encoding the same |
US6252137B1 (en) * | 1997-11-12 | 2001-06-26 | E. I. Du Pont De Nemours And Company | Soybean homolog of seed-specific transcription activator from Phaseolus vulgaris |
GB9806113D0 (en) * | 1998-03-20 | 1998-05-20 | Nickerson Biocem Ltd | Control |
US6936708B1 (en) * | 1998-04-09 | 2005-08-30 | Arizona Board Of Regents, Acting For And On Behalf Of Arizona State University | Expression of Alfin 1 and methods for producing transgenic plants having increased root growth and root specific gene activation |
WO1999055840A1 (en) * | 1998-04-27 | 1999-11-04 | The Regents Of The University Of California | A transcription factor and method for regulation of seed development, quality and stress-tolerance |
US6664446B2 (en) * | 1999-03-23 | 2003-12-16 | Mendel Biotechnology, Inc. | Transgenic plants comprising polynucleotides encoding transcription factors that confer disease tolerance |
EP1033405A3 (en) * | 1999-02-25 | 2001-08-01 | Ceres Incorporated | Sequence-determined DNA fragments and corresponding polypeptides encoded thereby |
BR0009471A (en) * | 1999-03-11 | 2002-12-17 | Genesis Res & Dev Corp Ltd | Compositions and processes for modifying gene transcription |
FR2799204B1 (en) * | 1999-10-01 | 2003-12-12 | Agronomique Inst Nat Rech | NEW CLASS OF PROTEINS AND THEIR APPLICATIONS TO THE RESISTANCE OF PLANTS TO VARIOUS PATHOGENS |
MXPA02003669A (en) * | 1999-10-12 | 2003-10-14 | Mendel Biotechnology Inc | Flowering time modification. |
EP1229782B1 (en) * | 1999-11-17 | 2012-11-07 | Mendel Biotechnology, Inc. | Pathogen tolerance genes |
EP1226240A2 (en) * | 2000-01-04 | 2002-07-31 | E.I. Du Pont De Nemours And Company | Spf1-related transcription factors |
CA2408038C (en) * | 2000-05-12 | 2013-10-01 | Cropdesign N.V. | Nucleic acid molecules encoding plant cell cycle proteins and uses therefor |
US20020194646A1 (en) * | 2000-07-20 | 2002-12-19 | Pogue Gregory P. | Methods of creating dwarf phenotypes in plants |
US7291767B2 (en) * | 2000-07-20 | 2007-11-06 | The Dow Chemical Company | Nucleic acids compositions conferring dwarfing phenotype |
WO2002015675A1 (en) * | 2000-08-22 | 2002-02-28 | Mendel Biotechnology, Inc. | Genes for modifying plant traits iv |
CA2420555C (en) * | 2000-08-24 | 2012-10-23 | Jeffrey F. Harper | Stress-regulated genes of plants, transgenic plants containing same, and methods of use |
WO2002022675A2 (en) * | 2000-09-15 | 2002-03-21 | Syngenta Participations Ag | Plant genes, the expression of which are altered by pathogen infection |
TWI552751B (en) | 2011-06-20 | 2016-10-11 | H 朗德貝克公司 | Method of administration of 4-((1r,3s)-6-chloro-3-phenyl-indan-1-yl)-1,2,2-trimethyl-piperazine and the salts thereof in the treatment of schizophrenia |
-
2000
- 2000-11-14 EP EP00982129A patent/EP1229782B1/en not_active Expired - Lifetime
- 2000-11-14 MX MXPA02004881A patent/MXPA02004881A/en unknown
- 2000-11-14 WO PCT/US2000/031457 patent/WO2001035727A1/en active Application Filing
- 2000-11-14 CA CA002391443A patent/CA2391443A1/en not_active Abandoned
- 2000-11-14 CA CA002390594A patent/CA2390594A1/en not_active Abandoned
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- 2000-11-14 BR BR0015631-0A patent/BR0015631A/en not_active Application Discontinuation
- 2000-11-14 AU AU17656/01A patent/AU1765601A/en not_active Abandoned
- 2000-11-14 AU AU17671/01A patent/AU1767101A/en not_active Abandoned
- 2000-11-14 AT AT00980418T patent/ATE398182T1/en not_active IP Right Cessation
- 2000-11-14 AU AU17683/01A patent/AU780463B2/en not_active Ceased
- 2000-11-14 WO PCT/US2000/031418 patent/WO2001035726A1/en active Application Filing
- 2000-11-14 WO PCT/US2000/031344 patent/WO2001036597A1/en active Application Filing
- 2000-11-14 PT PT00980418T patent/PT1230345E/en unknown
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- 2000-11-14 EP EP00980390A patent/EP1230344B1/en not_active Expired - Lifetime
- 2000-11-14 DE DE60039184T patent/DE60039184D1/en not_active Expired - Lifetime
- 2000-11-14 EP EP00978661A patent/EP1230256A4/en not_active Withdrawn
- 2000-11-14 AU AU19199/01A patent/AU1919901A/en not_active Abandoned
- 2000-11-14 WO PCT/US2000/031414 patent/WO2001035725A1/en not_active Application Discontinuation
- 2000-11-14 BR BR0015633-7A patent/BR0015633A/en not_active Application Discontinuation
- 2000-11-14 MX MXPA02004882A patent/MXPA02004882A/en unknown
- 2000-11-14 CA CA002391569A patent/CA2391569A1/en not_active Abandoned
- 2000-11-14 CA CA002390600A patent/CA2390600A1/en not_active Abandoned
- 2000-11-14 MX MXPA02004870A patent/MXPA02004870A/en unknown
- 2000-11-14 CA CA002391446A patent/CA2391446A1/en not_active Abandoned
- 2000-11-14 CA CA002390597A patent/CA2390597A1/en not_active Abandoned
- 2000-11-14 EP EP00980418A patent/EP1230345B1/en not_active Expired - Lifetime
- 2000-11-14 EP EP00980406A patent/EP1229780A4/en not_active Withdrawn
- 2000-11-14 MX MXPA02004878A patent/MXPA02004878A/en unknown
- 2000-11-14 MX MXPA02004880A patent/MXPA02004880A/en unknown
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- 2000-11-14 BR BR0015632-9A patent/BR0015632A/en not_active Application Discontinuation
Non-Patent Citations (5)
Title |
---|
AOYAMA T. ET AL.: "Ectopic expression of the arabidopsis transciptional activator Athb-1 alters leaf cell fate in tobacco", THE PLANT CELL, vol. 7, November 1995 (1995-11-01), pages 1773 - 1785, XP002938565 * |
BELLIS L.D. ET AL.: "Distinct cis-acting sequences are required for the germination and sugar responses of the cucumber isocitrate lyase gene", GENE, vol. 197, 1997, pages 375 - 378, XP002938566 * |
DATABASE GENBANK [online] US NATIONAL LIBRARY OF MEDICINE, (BETHESDA, MD, USA); 5 April 2000 (2000-04-05), LIN X. ET AL.: "Sequence and analysis of chromosome 2 of the plant arabidopsis thaliana", XP002938568, accession no. NCBI Database accession no. U78721 * |
KIM S. ET AL.: "Sugar response element enhances wound response of potato proteinase inhibitor IIpromoter in transgenic tobacco", PLANT MOL. BIOL., vol. 17, 1991, pages 973 - 983, XP002938567 * |
NATURE, vol. 402, no. 6763, 1999, pages 761 - 768 * |
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