WO2001032829A3 - Methods to screen microorganisms or gene libraries for products secreted from a cell - Google Patents

Methods to screen microorganisms or gene libraries for products secreted from a cell Download PDF

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Publication number
WO2001032829A3
WO2001032829A3 PCT/DK2000/000566 DK0000566W WO0132829A3 WO 2001032829 A3 WO2001032829 A3 WO 2001032829A3 DK 0000566 W DK0000566 W DK 0000566W WO 0132829 A3 WO0132829 A3 WO 0132829A3
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WO
WIPO (PCT)
Prior art keywords
fluorescence
interest
sample
step
host cell
Prior art date
Application number
PCT/DK2000/000566
Other languages
French (fr)
Other versions
WO2001032829A2 (en
Inventor
Soeren Moeller
Lars Kongsbak
Hans-Henrik Kristensen
Jesper Vind
Henrik Pedersen
Tommy Lykke Husum
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DKPA199901602 priority Critical
Priority to DKPA199901602 priority
Application filed by Novozymes As filed Critical Novozymes As
Publication of WO2001032829A2 publication Critical patent/WO2001032829A2/en
Publication of WO2001032829A3 publication Critical patent/WO2001032829A3/en

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Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries

Abstract

The invention describes methods for screening for products secreted from the cells, and provides methods to establish a correlation between the activity of the secreted product and the secreting cell. Accordingly in a first aspect the present invention relates to a method for screening a DNA library for DNA of interest comprising the steps of a) creating host cells comprising the DNA library, b) generating samples each comprising a host cell of step a), c) establishing a means for correlating host cell secretion of a material of interest in a sample with the fluorescence of the sample, d) determining which intensity interval of fluorescence indicates secretion in the sample when the correlating means of step c) is used, e) cultivating the samples under suitable conditions, and f) selecting the samples exhibiting fluorescence within the intensity interval of step d) using a fluorescence analyzer; wherein the host cell comprises DNA of interest.
PCT/DK2000/000566 1999-11-05 2000-10-10 Methods to screen microorganisms or gene libraries for products secreted from a cell WO2001032829A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DKPA199901602 1999-11-05
DKPA199901602 1999-11-05

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AU76460/00A AU7646000A (en) 1999-11-05 2000-10-10 Methods to screen microorganisms or gene libraries for products secreted from a cell

Publications (2)

Publication Number Publication Date
WO2001032829A2 WO2001032829A2 (en) 2001-05-10
WO2001032829A3 true WO2001032829A3 (en) 2001-12-13

Family

ID=8106447

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DK2000/000566 WO2001032829A2 (en) 1999-11-05 2000-10-10 Methods to screen microorganisms or gene libraries for products secreted from a cell

Country Status (2)

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AU (1) AU7646000A (en)
WO (1) WO2001032829A2 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
AT501251T (en) 2001-01-25 2011-03-15 Evolva Ltd cell library
WO2004090549A1 (en) * 2003-04-11 2004-10-21 Novozymes A/S Method of screening for secreted glycosylated polypeptides
EP2044440A4 (en) * 2006-06-28 2014-04-02 Ge Healthcare Bio Sciences Ab Method of detecting interactions between protein components
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
AU2016218254A1 (en) 2015-02-09 2017-08-17 Slingshot Biosciences, Inc. Hydrogel particles with tunable optical properties and methods for using the same

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
WO1999031277A1 (en) * 1997-12-15 1999-06-24 Medical Science Systems, Inc. Expression cloning and single cell detection of phenotype
WO1999037807A1 (en) * 1998-01-26 1999-07-29 European Molecular Biology Laboratory Method of isolation of rna-binding compounds
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1997020078A1 (en) * 1995-11-30 1997-06-05 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5965408A (en) * 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
WO1998058085A1 (en) * 1997-06-16 1998-12-23 Diversa Corporation High throughput screening for novel enzymes
WO1999031277A1 (en) * 1997-12-15 1999-06-24 Medical Science Systems, Inc. Expression cloning and single cell detection of phenotype
WO1999037807A1 (en) * 1998-01-26 1999-07-29 European Molecular Biology Laboratory Method of isolation of rna-binding compounds

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
C.J. CID ET AL.: "Yeast exo-beta-glucanases can be used as efficient and readily detectable reporter genes in saccharomyces cerevisiae", YEAST, vol. 10, 1994, pages 747 - 756 *
K.D. WITTRUP ET AL.: "Microencapsulation selection for isolation of yeast mutants with increased secretion of aspergillus awamori glucoamylase", BIOTECHNOLOGY AND BIOENGINEERING, vol. 42, 1993, pages 351 - 356 *

Also Published As

Publication number Publication date
AU7646000A (en) 2001-05-14
WO2001032829A2 (en) 2001-05-10

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