WO2001031057A3 - Double-strand nucleic acid probes and the use thereof - Google Patents

Double-strand nucleic acid probes and the use thereof Download PDF

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Publication number
WO2001031057A3
WO2001031057A3 PCT/EP2000/010209 EP0010209W WO0131057A3 WO 2001031057 A3 WO2001031057 A3 WO 2001031057A3 EP 0010209 W EP0010209 W EP 0010209W WO 0131057 A3 WO0131057 A3 WO 0131057A3
Authority
WO
WIPO (PCT)
Prior art keywords
double
use
nucleic acid
strand nucleic
acid probes
Prior art date
Application number
PCT/EP2000/010209
Other languages
German (de)
French (fr)
Other versions
WO2001031057A2 (en
Inventor
Jochen Muth
Norbert Windhab
Original Assignee
Aventis Res & Tech Gmbh & Co
Jochen Muth
Norbert Windhab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to DE19950969A priority Critical patent/DE19950969A1/en
Priority to DE19950969.7 priority
Application filed by Aventis Res & Tech Gmbh & Co, Jochen Muth, Norbert Windhab filed Critical Aventis Res & Tech Gmbh & Co
Publication of WO2001031057A2 publication Critical patent/WO2001031057A2/en
Publication of WO2001031057A3 publication Critical patent/WO2001031057A3/en

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/6825Nucleic acid detection involving sensors

Abstract

The invention relates to electronically-isolable double-strand nucleic acid probes and use thereof for rapid and easy detection of interactions between double-stranded nucleic acids and factors which interact with them either by mediated or direct means. The invention further relates to the production of said double-strand nucleic acids.
PCT/EP2000/010209 1999-10-22 2000-10-17 Double-strand nucleic acid probes and the use thereof WO2001031057A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
DE19950969A DE19950969A1 (en) 1999-10-22 1999-10-22 Double-stranded nucleic acid probes and their use
DE19950969.7 1999-10-22

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
CA002387567A CA2387567A1 (en) 1999-10-22 2000-10-17 Double-stranded nucleic acid probes and their use
KR1020027005181A KR20020065488A (en) 1999-10-22 2000-10-17 Double-strand nucleic acid probes and the use thereof
BR0015240-4A BR0015240A (en) 1999-10-22 2000-10-17 nucleic acid double-stranded probes and their application
EP00969512A EP1226272A2 (en) 1999-10-22 2000-10-17 Double-strand nucleic acid probes and the use thereof
JP2001533192A JP2003512081A (en) 1999-10-22 2000-10-17 Double-stranded nucleic acid probes and their use
AU79201/00A AU7920100A (en) 1999-10-22 2000-10-17 Double-strand nucleic acid probes and the use thereof

Publications (2)

Publication Number Publication Date
WO2001031057A2 WO2001031057A2 (en) 2001-05-03
WO2001031057A3 true WO2001031057A3 (en) 2001-12-27

Family

ID=7926558

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2000/010209 WO2001031057A2 (en) 1999-10-22 2000-10-17 Double-strand nucleic acid probes and the use thereof

Country Status (8)

Country Link
EP (1) EP1226272A2 (en)
JP (1) JP2003512081A (en)
KR (1) KR20020065488A (en)
AU (1) AU7920100A (en)
BR (1) BR0015240A (en)
CA (1) CA2387567A1 (en)
DE (1) DE19950969A1 (en)
WO (1) WO2001031057A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1558626A4 (en) * 2002-10-11 2007-10-31 Ahram Biosystems Inc Target detection system having a conformationally sensitive probe comprising a nucleic acid based signal transducer
WO2004106545A1 (en) * 2003-05-28 2004-12-09 Innogenetics N.V. Methods for enhanced detection using surface sensitive techniques.
CA2770071C (en) * 2009-08-07 2014-07-15 Ohmx Corporation Enzyme triggered redox altering chemical elimination (e-trace) immunoassay
US8877022B2 (en) * 2011-06-21 2014-11-04 National Taiwan University Biosensor

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002981A1 (en) * 1989-08-25 1991-03-07 Amersham International Plc Assay method
WO1996040712A1 (en) * 1995-06-07 1996-12-19 California Institute Of Technology Nucleic acid mediated electron transfer
WO1997004129A1 (en) * 1995-07-14 1997-02-06 Biacore Ab Method for nucleic acid sequencing
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
WO1998031839A2 (en) * 1997-01-21 1998-07-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
WO1999015893A1 (en) * 1997-09-22 1999-04-01 Aventis Research & Technologies Gmbh & Co. Kg Addressable modular recognition system, production mode and use
WO1999031275A1 (en) * 1997-12-15 1999-06-24 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand diagnostic biochip
DE19901761A1 (en) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Oligonucleotides tagged with photoinducible redox-active unit
WO2001013126A1 (en) * 1999-08-13 2001-02-22 Nanogen, Inc. Microelectronic molecular descriptor array devices, methods, procedures, and formats for combinatorial selection of intermolecular ligand binding structures and for drug screening

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU7011898A (en) * 1997-04-22 1998-11-13 Thomas Schalkhammer Reinforced cluster optical sensors

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991002981A1 (en) * 1989-08-25 1991-03-07 Amersham International Plc Assay method
WO1996040712A1 (en) * 1995-06-07 1996-12-19 California Institute Of Technology Nucleic acid mediated electron transfer
WO1997004129A1 (en) * 1995-07-14 1997-02-06 Biacore Ab Method for nucleic acid sequencing
WO1998020162A2 (en) * 1996-11-05 1998-05-14 Clinical Micro Sensors Electrodes linked via conductive oligomers to nucleic acids
WO1998031839A2 (en) * 1997-01-21 1998-07-23 President And Fellows Of Harvard College Electronic-property probing of biological molecules at surfaces
WO1999015893A1 (en) * 1997-09-22 1999-04-01 Aventis Research & Technologies Gmbh & Co. Kg Addressable modular recognition system, production mode and use
WO1999031275A1 (en) * 1997-12-15 1999-06-24 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand diagnostic biochip
DE19901761A1 (en) * 1999-01-18 1999-07-01 Gerhard Dr Hartwich Oligonucleotides tagged with photoinducible redox-active unit
WO2001013126A1 (en) * 1999-08-13 2001-02-22 Nanogen, Inc. Microelectronic molecular descriptor array devices, methods, procedures, and formats for combinatorial selection of intermolecular ligand binding structures and for drug screening

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ERSKINE SYMON G ET AL: "Reactions on the EcoRV restriction endonuclease with fluorescent oligodeoxynucleotides: Identical equilibrium constants for binding to specific and non-specific DNA.", JOURNAL OF MOLECULAR BIOLOGY, vol. 275, no. 5, 6 February 1998 (1998-02-06), pages 759 - 772, XP002173203, ISSN: 0022-2836 *
GAO HETIAN ET AL: "Stabilization of double-stranded oligonucleotides using backbone-linked disulfide bridges.", NUCLEIC ACIDS RESEARCH, vol. 23, no. 2, 1995, pages 285 - 292, XP002173239, ISSN: 0305-1048 *
HIBMA M H ET AL: "A NON-RADIOACTIVE ASSAY FOR THE DETECTION AND QUANTITATION OF A DNA BINDING PROTEIN", NUCLEIC ACIDS RESEARCH,GB,OXFORD UNIVERSITY PRESS, SURREY, vol. 22, no. 18, 11 September 1994 (1994-09-11), pages 3806 - 3807, XP000673300, ISSN: 0305-1048 *
OKAHATA YOSHIO ET AL: "Kinetic studies of sequence-specific binding of GCN4-bZIP peptides to DNA strands immobilized on a 27-MHz quartz-crystal microbalance.", BIOCHEMISTRY, vol. 37, no. 16, 21 April 1998 (1998-04-21), pages 5666 - 5672, XP002173202, ISSN: 0006-2960 *

Also Published As

Publication number Publication date
CA2387567A1 (en) 2001-05-03
DE19950969A1 (en) 2001-05-10
WO2001031057A2 (en) 2001-05-03
JP2003512081A (en) 2003-04-02
AU7920100A (en) 2001-05-08
EP1226272A2 (en) 2002-07-31
BR0015240A (en) 2002-07-16
KR20020065488A (en) 2002-08-13

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