WO2001027624A3 - Compositions and methods for detecting protein modification and enzymatic activity - Google Patents

Compositions and methods for detecting protein modification and enzymatic activity Download PDF

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Publication number
WO2001027624A3
WO2001027624A3 PCT/US2000/041062 US0041062W WO0127624A3 WO 2001027624 A3 WO2001027624 A3 WO 2001027624A3 US 0041062 W US0041062 W US 0041062W WO 0127624 A3 WO0127624 A3 WO 0127624A3
Authority
WO
WIPO (PCT)
Prior art keywords
modification
protein
protein modification
target protein
detecting
Prior art date
Application number
PCT/US2000/041062
Other languages
French (fr)
Other versions
WO2001027624A2 (en
Inventor
Li Shen
Debra Hui Cen
Original Assignee
Superarray Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Superarray Inc filed Critical Superarray Inc
Priority to EP00978895A priority Critical patent/EP1224463A2/en
Priority to AU16305/01A priority patent/AU1630501A/en
Priority to CA002383945A priority patent/CA2383945A1/en
Publication of WO2001027624A2 publication Critical patent/WO2001027624A2/en
Publication of WO2001027624A3 publication Critical patent/WO2001027624A3/en

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

This invention relates generally to the field of protein modification, e.g., post-translational modification. In particular, the invention provides a method for detecting protein modification profile in a sample, which method comprises: a) contacting a sample containing or suspected of containing a target protein with a capture molecule, or a plurality of capture molecules, immobilized on a solid support, said capture molecule is capable of specifically binding to said target protein, whereby said target protein is immobilized on said solid support; and b) assessing modification status and/or identity of said immobilized target protein. Kits and arrays useful for detecting protein modification are also provided. Arrays, kits and methods useful for detecting enzymatic activities, especially protein modification enzymatic activities, are further provided.
PCT/US2000/041062 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity WO2001027624A2 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP00978895A EP1224463A2 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity
AU16305/01A AU1630501A (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity
CA002383945A CA2383945A1 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15856099P 1999-10-08 1999-10-08
US60/158,560 1999-10-08

Publications (2)

Publication Number Publication Date
WO2001027624A2 WO2001027624A2 (en) 2001-04-19
WO2001027624A3 true WO2001027624A3 (en) 2002-02-21

Family

ID=22568698

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/041062 WO2001027624A2 (en) 1999-10-08 2000-10-03 Compositions and methods for detecting protein modification and enzymatic activity

Country Status (5)

Country Link
US (1) US20030153014A1 (en)
EP (1) EP1224463A2 (en)
AU (1) AU1630501A (en)
CA (1) CA2383945A1 (en)
WO (1) WO2001027624A2 (en)

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US20070003954A1 (en) * 2005-05-12 2007-01-04 The Board Of Regents Of The University Of Texas System Protein and antibody profiling using small molecule microarrays
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Also Published As

Publication number Publication date
US20030153014A1 (en) 2003-08-14
EP1224463A2 (en) 2002-07-24
CA2383945A1 (en) 2001-04-19
WO2001027624A2 (en) 2001-04-19
AU1630501A (en) 2001-04-23

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