WO2001023595A3 - Reduced gravity transformation process and product - Google Patents

Reduced gravity transformation process and product Download PDF

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Publication number
WO2001023595A3
WO2001023595A3 PCT/US2000/026899 US0026899W WO0123595A3 WO 2001023595 A3 WO2001023595 A3 WO 2001023595A3 US 0026899 W US0026899 W US 0026899W WO 0123595 A3 WO0123595 A3 WO 0123595A3
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WO
WIPO (PCT)
Prior art keywords
cell
reduced gravity
gene
gravity
product
Prior art date
Application number
PCT/US2000/026899
Other languages
French (fr)
Other versions
WO2001023595A2 (en
Inventor
Stephen L Goldman
Richard A Vierling
Raymond J Bula
Original Assignee
Univ Toledo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Toledo filed Critical Univ Toledo
Priority to AU77386/00A priority Critical patent/AU7738600A/en
Publication of WO2001023595A2 publication Critical patent/WO2001023595A2/en
Publication of WO2001023595A3 publication Critical patent/WO2001023595A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Abstract

A transformation process is carried out in which the competence of a plant or animal cell is increased and/or induced by placing the cell in reduced gravity, and then the cell is transformed in reduced gravity. A transformation process is carried out in which a monocot plant cell is transformed in reduced gravity. In a process of increasing the frequency of transformation of cells, the cells are contacted with a chemical agent effective to increase the frequency of transformation of the cells, and then the cells are transformed in reduced gravity. In a process of providing an increased amount of transgenic gene product produced, a cell is provided having a gene which has been transformed into the cell by a transformation process. Then, gene product is produced from the gene in reduced gravity. A product is provided relating to a plant or animal cell having DNA transferred into the cell in reduced gravity. A cell has a chemical pathway enhanced by a gene product produced in reduced gravity from a gene transferred into the cell in either reduced gravity or atmospheric gravity. The transferred gene produces an increased amount of product or an altered product compared to the same gene product produced in atmospheric gravity.
PCT/US2000/026899 1999-09-30 2000-09-29 Reduced gravity transformation process and product WO2001023595A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU77386/00A AU7738600A (en) 1999-09-30 2000-09-29 Reduced gravity transformation process and product

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US15689199P 1999-09-30 1999-09-30
US60/156,891 1999-09-30
US15927899P 1999-10-13 1999-10-13
US60/159,278 1999-10-13

Publications (2)

Publication Number Publication Date
WO2001023595A2 WO2001023595A2 (en) 2001-04-05
WO2001023595A3 true WO2001023595A3 (en) 2002-02-14

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/026899 WO2001023595A2 (en) 1999-09-30 2000-09-29 Reduced gravity transformation process and product

Country Status (2)

Country Link
AU (1) AU7738600A (en)
WO (1) WO2001023595A2 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009137135A2 (en) * 2008-02-15 2009-11-12 Zgi, Inc. On-orbit procedures for adapting plants and animals to hostile environments
WO2008067422A2 (en) * 2006-11-28 2008-06-05 Zgi, Inc. Replication of undifferentiated diploid cells in a weightless environment, uses thereof and a facility for such replication
BRPI0802399B1 (en) * 2008-07-08 2018-06-12 União Brasileira De Educação E Assistência- Mantenedora Da Pucrs FISH AND OTHER ANIMAL INCUBATION EQUIPMENT AND FISH AND OTHER ANIMAL CULTIVATION PROCESS
CN108165572A (en) * 2017-11-07 2018-06-15 河南农业大学 A kind of agriculture bacillus mediated needle thorn growing point transformed wheat method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4954442A (en) * 1988-08-31 1990-09-04 Purdue Research Foundation Opine enhancement of vir gene induction
WO1994015630A1 (en) * 1993-01-13 1994-07-21 Yeda Research And Development Co. Ltd. Chemotactic factors for human spermatozoa and their use in human assisted fertilization
US5376543A (en) * 1987-12-21 1994-12-27 The University Of Toledo Agrobacterium mediated transformation of germinating plant seeds

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5376543A (en) * 1987-12-21 1994-12-27 The University Of Toledo Agrobacterium mediated transformation of germinating plant seeds
US4954442A (en) * 1988-08-31 1990-09-04 Purdue Research Foundation Opine enhancement of vir gene induction
WO1994015630A1 (en) * 1993-01-13 1994-07-21 Yeda Research And Development Co. Ltd. Chemotactic factors for human spermatozoa and their use in human assisted fertilization

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
BULA R J ET AL.: "Genetic engineering in a microgravity environment", CHEMICAL INNOVATION, vol. 30, no. 1, January 2000 (2000-01-01), pages 30 - 33, XP000993411 *
CIFERRI O ET AL: "EFFECTS OF MICROGRAVITY ON GENETIC RECOMBINATION IN ESCHERICHIA-COLI", NATURWISSENSCHAFTEN, vol. 73, no. 7, 1986, pages 418 - 421, XP000993281, ISSN: 0028-1042 *
DONG L -Y ET AL: "Enhancing effects of simulated microgravity on Agrobacterium-infected frequency of tobacco callus.", MICROGRAVITY SCIENCE AND TECHNOLOGY, vol. 7, no. 4, 1995, pages 336 - 338, XP001002118, ISSN: 0938-0108 *
HOFFMANN E ET AL: "Electrofusion of plant cell protoplasts under microgravity: A D-2 Spacelab experiment.", MICROGRAVITY SCIENCE AND TECHNOLOGY, vol. 8, no. 3, 1995, pages 188 - 195, XP000993425, ISSN: 0938-0108 *
TENNESSEN D J: "Problems associated with gene transfer and opportunities for microgravity environments", AMERICAN INSTITUTE OF PHYSICS CONFERENCE PROCEEDINGS, vol. 387, no. 1, 1997, pages 1019 - 1024, XP000993314 *

Also Published As

Publication number Publication date
WO2001023595A2 (en) 2001-04-05
AU7738600A (en) 2001-04-30

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