WO2001020042A3 - Method for processing biological samples - Google Patents

Method for processing biological samples Download PDF

Info

Publication number
WO2001020042A3
WO2001020042A3 PCT/US2000/025423 US0025423W WO0120042A3 WO 2001020042 A3 WO2001020042 A3 WO 2001020042A3 US 0025423 W US0025423 W US 0025423W WO 0120042 A3 WO0120042 A3 WO 0120042A3
Authority
WO
WIPO (PCT)
Prior art keywords
separation
target species
biological samples
processing biological
capture
Prior art date
Application number
PCT/US2000/025423
Other languages
French (fr)
Other versions
WO2001020042A2 (en
Inventor
William H Chapman Jr
Leonard Klevan
Original Assignee
Miraibio Inc
William H Chapman Jr
Leonard Klevan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miraibio Inc, William H Chapman Jr, Leonard Klevan filed Critical Miraibio Inc
Priority to JP2001523811A priority Critical patent/JP2003509680A/en
Priority to EP00961946A priority patent/EP1212465A2/en
Priority to AU73829/00A priority patent/AU7382900A/en
Priority to CA002383415A priority patent/CA2383415A1/en
Publication of WO2001020042A2 publication Critical patent/WO2001020042A2/en
Publication of WO2001020042A3 publication Critical patent/WO2001020042A3/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Methods, compositions and systems for processing biological samples employ microparticulate separation reagents to capture a target species from a biological sample. Photoactivation of a photoaffinity label coupled to the separation reagent creates a covalent adduct of the separation reagent and target species. The adduct is captured and DNA from the target species is analyzed using known techniques. Capture is accomplished using magnetic beads, filtration, centrifugation or other separation techniques. The method of the invention is amenable to complete automation.
PCT/US2000/025423 1999-09-15 2000-09-15 Method for processing biological samples WO2001020042A2 (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2001523811A JP2003509680A (en) 1999-09-15 2000-09-15 Methods for processing biological samples
EP00961946A EP1212465A2 (en) 1999-09-15 2000-09-15 Method for processing biological samples
AU73829/00A AU7382900A (en) 1999-09-15 2000-09-15 Method for processing biological samples
CA002383415A CA2383415A1 (en) 1999-09-15 2000-09-15 Method for processing biological samples

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US15414899P 1999-09-15 1999-09-15
US60/154,148 1999-09-15

Publications (2)

Publication Number Publication Date
WO2001020042A2 WO2001020042A2 (en) 2001-03-22
WO2001020042A3 true WO2001020042A3 (en) 2001-11-22

Family

ID=22550197

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/025423 WO2001020042A2 (en) 1999-09-15 2000-09-15 Method for processing biological samples

Country Status (5)

Country Link
EP (1) EP1212465A2 (en)
JP (1) JP2003509680A (en)
AU (1) AU7382900A (en)
CA (1) CA2383415A1 (en)
WO (1) WO2001020042A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5773405B2 (en) * 2010-07-09 2015-09-02 国立大学法人高知大学 Method for detecting compounds that interact with molecules on cell membranes
WO2017175523A1 (en) 2016-04-06 2017-10-12 コニカミノルタ株式会社 Fluorescent immunostaining method

Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0156537A2 (en) * 1984-03-02 1985-10-02 Board Of Regents University Of Texas System Biological magnetic fluids
US4716122A (en) * 1984-09-28 1987-12-29 Organogen Medizinisch-Molekularbiologische Forschungsgesellschaft M.B.H. Carrier material for use in immune determinations
EP0332022A2 (en) * 1988-03-10 1989-09-13 BEHRINGWERKE Aktiengesellschaft Magnetic protein conjugates, process for their production and their use
US4879220A (en) * 1986-11-18 1989-11-07 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Crosslinking receptor-specific probes for electron microscopy
EP0408078A2 (en) * 1989-07-14 1991-01-16 Roche Diagnostics GmbH Method for the preparation of a solid phase coated with an immunologically active substance
EP0475784A1 (en) * 1990-09-14 1992-03-18 Biosite Diagnostics Inc. Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays
WO1994001773A1 (en) * 1992-07-06 1994-01-20 Biomira Inc. Photoactivation of proteins for conjugation purposes
WO1995016204A1 (en) * 1993-12-07 1995-06-15 University Court Of The University Of Glasgow Surface-patterned device
WO1995031429A1 (en) * 1994-05-11 1995-11-23 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
WO1996031781A1 (en) * 1995-04-01 1996-10-10 Boehringer Mannheim Gmbh System for releasing and isolating nucleic acids

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0156537A2 (en) * 1984-03-02 1985-10-02 Board Of Regents University Of Texas System Biological magnetic fluids
US4716122A (en) * 1984-09-28 1987-12-29 Organogen Medizinisch-Molekularbiologische Forschungsgesellschaft M.B.H. Carrier material for use in immune determinations
US4879220A (en) * 1986-11-18 1989-11-07 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Crosslinking receptor-specific probes for electron microscopy
EP0332022A2 (en) * 1988-03-10 1989-09-13 BEHRINGWERKE Aktiengesellschaft Magnetic protein conjugates, process for their production and their use
EP0408078A2 (en) * 1989-07-14 1991-01-16 Roche Diagnostics GmbH Method for the preparation of a solid phase coated with an immunologically active substance
EP0475784A1 (en) * 1990-09-14 1992-03-18 Biosite Diagnostics Inc. Antibodies to complexes of ligand receptors and ligands and their utility in ligand-receptor assays
WO1994001773A1 (en) * 1992-07-06 1994-01-20 Biomira Inc. Photoactivation of proteins for conjugation purposes
WO1995016204A1 (en) * 1993-12-07 1995-06-15 University Court Of The University Of Glasgow Surface-patterned device
WO1995031429A1 (en) * 1994-05-11 1995-11-23 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
WO1996031781A1 (en) * 1995-04-01 1996-10-10 Boehringer Mannheim Gmbh System for releasing and isolating nucleic acids

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
EBERLE A N: "PHOTO AFFINITY LABELING OF PEPTIDE HORMONE RECEPTORS", JOURNAL OF RECEPTOR RESEARCH, vol. 3, no. 1-2, 1983, pages 313 - 326, XP001009839, ISSN: 0197-5110 *
KAUSCH ALBERT P ET AL: "Organelle isolation by magnetic immunoabsorption.", BIOTECHNIQUES, vol. 26, no. 2, February 1999 (1999-02-01), pages 336 - 343, XP002170373, ISSN: 0736-6205 *
MARGEL ET AL.: "Functional nanospheres: synthesis and biological applications", RECENT RES.DEVEL.IN POLYMER SCIENCE, vol. 1, 1997, pages 51 - 78, XP001009817 *
MARGEL SHLOMO ET AL: "Synthesis, characterization, and use of new solid and hollow, magnetic and non-magnetic, organic-inorganic monodispersed hybrid microspheres.", SCIENTIFIC AND CLINICAL APPLICATIONS OF MAGNETIC CARRIERS., 1997, International Conference;Rostock, Germany; September 5-7, 1996, Plenum Press;Plenum Press 233 Spring Street, New York, New York, USA; London, England, UK, pages 37 - 51, XP001009773, ISBN: 0-306-45687-7 *
SAFARK I ET AL: "Use of magnetic techniques for the isolation of cells", JOURNAL OF CHROMATOGRAPHY B: BIOMEDICAL APPLICATIONS,NL,ELSEVIER SCIENCE PUBLISHERS, vol. 722, no. 1-2, 5 February 1999 (1999-02-05), pages 33 - 53, XP004156204, ISSN: 0378-4347 *

Also Published As

Publication number Publication date
AU7382900A (en) 2001-04-17
CA2383415A1 (en) 2001-03-22
WO2001020042A2 (en) 2001-03-22
JP2003509680A (en) 2003-03-11
EP1212465A2 (en) 2002-06-12

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