WO2001012844A1 - Rapid method for measuring complex carbohydrates in mammalian tissue - Google Patents
Rapid method for measuring complex carbohydrates in mammalian tissue Download PDFInfo
- Publication number
- WO2001012844A1 WO2001012844A1 PCT/NZ2000/000159 NZ0000159W WO0112844A1 WO 2001012844 A1 WO2001012844 A1 WO 2001012844A1 NZ 0000159 W NZ0000159 W NZ 0000159W WO 0112844 A1 WO0112844 A1 WO 0112844A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- complex carbohydrates
- measuring complex
- rapid method
- measuring
- measurement
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/40—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving amylase
Definitions
- the present invention relates to a rapid method for measuring complex carbohydrates
- glycogen in mammalian tissue. More particularly the present invention relates
- glycogen in mammalian tissue.
- the measurement of glycogen includes, for example, the ability to use the results of
- glycogen measurement as a determination of the ultimate pH of meat. This in turn is a direct
- the iodine method The principle of this method is that glycogen will react with a mixture
- glycogen is extracted from the
- glycogen which is reacted with the iodine can also be by liquid nitrogen,
- amyloglucosidase method of Dreiling et al (Meat Science, Vol 20, p. 167) is one
- a muscle sample is homogenised with
- Amyloglucosidase is added, converting the glycogen to glucose, for
- the first part of the method takes approximately 30 minutes at 37°C.
- the method also utilise mild or non-aggressive reagents.
- low glycogen levels in the liver of bobby calves are an
- rapid is used to refer to times of less than
- said complex carbohydrate is glycogen.
- solvent is water.
- said solvent may include at least one agent intended to
- steps (b) and (c) are performed simultaneously.
- steps (c) and (d) are performed simultaneously.
- the hydrolysing enzyme may be any enzyme, or combination of enzymes,
- hydrolysing enzyme is selected from the group: amyloglucosidase; -amylase;
- ⁇ -glucosidase ⁇ -glucosidase
- step (c) measuring complex carbohydrates in mammalian tissue, substantially as described above, wherein the amyloglucosidase added in step (c) is in a form selected from: a powder; a liquid
- said method further includes a step (e): measuring the
- steps (d) and (e) are performed simultaneously.
- steps (c), (d) and (e) are performed simultaneously.
- the method is conducted at approximately room temperature, although it is not
- concentration of glucose is achieved by the construction of sensors incorporating said
- hydrolysing enzyme and glucose oxidase hydrolysing enzyme and glucose oxidase.
- lactate as catalysed by lactate oxidase.
- This latter compound can be incorporated into any combination
- Figure 1 is a graph of glucose concentration and glucometer reading of a glucose
- Figure 2 is a graph of the glucometer reading for glucose and concentration of glucose added to meat slurry samples
- Figure 3 is a graph of the kinetics of glucose formation from glycogen in acetate buffer
- Figure 4 is a graph of glucometer readings and the concentration of glucose added to
- Figure 5 is a graph of glucose value and glycogen added to a post rigor meat slurry, at
- Figure 6 is a graph of muscle glycogen concentration in post slaughter samples, using
- Figure 7 is a graph showing the kinetics of glycogen loss, pH fall and lactate increase
- Figure 8 is a graph of the repeatability of glycogen determination by the method of the
- solution can be obtained by the use of 25 mg of powder, dissolved in 5 ml of 0.2M sodium acetate at a pH of 4.5. ⁇ -D-Glucose of a standard analytical grade was used.
- Glucose measurement was made with an Esprit glucometer (Bayer). Test sensors used in the
- Esprit were from Bayer New Zealand Limited. These were used for one reading only then
- the meat samples tested were obtained from the longissimus lumborum muscle of a beef
- test medium for all experiments was 0.2M sodium acetate buffer at a pH of 4.4 and at
- Polytron shearing head This was usually set at 25,000 ⁇ m. Alternatively a lower speed
- the homogeniser may be used, if so desired.
- the homogeniser may be a stainless steel paddle ⁇
- lactate concentration was also measured. At various times after
- amyloglucosidase was centrifuged in a micro-centrifuge (at 10, 000 ⁇ m for 30 seconds).
- the density of meat is about 1 g/ml.
- Example 1 The method of Example 1 was repeated over a range of samples and concentrations in which
- glycogen between 0 - 14 mg were added to 5 ml of acetate buffer and
- Meat samples ranged from between 0.97 - 1.07 g in six tests. The results are as
- gluteus maxims muscle sample contained 13.1 mg/g at 2.3 hours post slaughter
- This muscle is frequently used as an indicator of high pH condition. Lactate concentrations were also measured.
- NADH-linked method rather than the use of a sensor based on lactate oxidase.
- glycogen were added to three replicate rigor meat samples weighing 1.0 +/- 0.05 g.
- tissue samples may be equally treated in like manner to produce glycogen
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
Description
Claims
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP00955193A EP1210451A4 (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
NZ517442A NZ517442A (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
AU67425/00A AU779415C (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
BR0013395-7A BR0013395A (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
CA002382155A CA2382155A1 (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
NZ33727699 | 1999-08-17 | ||
NZ337276 | 1999-08-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001012844A1 true WO2001012844A1 (en) | 2001-02-22 |
Family
ID=19927450
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/NZ2000/000159 WO2001012844A1 (en) | 1999-08-17 | 2000-08-17 | Rapid method for measuring complex carbohydrates in mammalian tissue |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP1210451A4 (en) |
AR (1) | AR025303A1 (en) |
AU (1) | AU779415C (en) |
BR (1) | BR0013395A (en) |
CA (1) | CA2382155A1 (en) |
WO (1) | WO2001012844A1 (en) |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9475223B2 (en) | 2002-08-14 | 2016-10-25 | Muhammed Aslam Nasir | Method of manufacturing an airway device |
USD842456S1 (en) | 2015-12-15 | 2019-03-05 | Intersurgical Ag | Airway device |
US10625037B2 (en) | 2013-12-17 | 2020-04-21 | Intersurgical Ag | Intubating airway device |
US11701484B2 (en) | 2017-12-13 | 2023-07-18 | Ashkal Developments Limited | Airway device |
USD1025348S1 (en) | 2020-04-16 | 2024-04-30 | Intersurgical Ag | Airway device |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4467811A (en) * | 1979-08-02 | 1984-08-28 | Children's Hospital Medical Center | Method of polarographic analysis of lactic acid and lactate |
US5262305A (en) * | 1991-03-04 | 1993-11-16 | E. Heller & Company | Interferant eliminating biosensors |
-
2000
- 2000-08-17 EP EP00955193A patent/EP1210451A4/en not_active Withdrawn
- 2000-08-17 WO PCT/NZ2000/000159 patent/WO2001012844A1/en not_active Application Discontinuation
- 2000-08-17 AU AU67425/00A patent/AU779415C/en not_active Ceased
- 2000-08-17 CA CA002382155A patent/CA2382155A1/en not_active Abandoned
- 2000-08-17 AR ARP000104247 patent/AR025303A1/en unknown
- 2000-08-17 BR BR0013395-7A patent/BR0013395A/en not_active IP Right Cessation
Non-Patent Citations (2)
Title |
---|
CALDER P.C. AND GEDDES R.: "Post mortem glycogenolysis is a combination of phosphorolysis and hydrolysis", INT J. BIOCHEM., vol. 22, no. 8, 1990, pages 847 - 856, XP002953344 * |
See also references of EP1210451A4 * |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9475223B2 (en) | 2002-08-14 | 2016-10-25 | Muhammed Aslam Nasir | Method of manufacturing an airway device |
US10625037B2 (en) | 2013-12-17 | 2020-04-21 | Intersurgical Ag | Intubating airway device |
USD842456S1 (en) | 2015-12-15 | 2019-03-05 | Intersurgical Ag | Airway device |
USD877888S1 (en) | 2015-12-15 | 2020-03-10 | Intersurgical Ag | Airway device |
US11701484B2 (en) | 2017-12-13 | 2023-07-18 | Ashkal Developments Limited | Airway device |
USD1025348S1 (en) | 2020-04-16 | 2024-04-30 | Intersurgical Ag | Airway device |
Also Published As
Publication number | Publication date |
---|---|
BR0013395A (en) | 2002-07-09 |
AU6742500A (en) | 2001-03-13 |
CA2382155A1 (en) | 2001-02-22 |
AR025303A1 (en) | 2002-11-20 |
EP1210451A4 (en) | 2004-08-25 |
AU779415C (en) | 2006-02-09 |
EP1210451A1 (en) | 2002-06-05 |
AU779415B2 (en) | 2005-01-20 |
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