WO2001004135A3 - Crosslinked dna condensate compositions and gene delivery methods - Google Patents

Crosslinked dna condensate compositions and gene delivery methods Download PDF

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Publication number
WO2001004135A3
WO2001004135A3 PCT/US2000/019164 US0019164W WO0104135A3 WO 2001004135 A3 WO2001004135 A3 WO 2001004135A3 US 0019164 W US0019164 W US 0019164W WO 0104135 A3 WO0104135 A3 WO 0104135A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene delivery
compositions
delivery methods
crosslinked dna
dna condensate
Prior art date
Application number
PCT/US2000/019164
Other languages
French (fr)
Other versions
WO2001004135A2 (en
Inventor
Kevin G Rice
Roger C Adami
Donald L Mckenzie
Wendy T Collard
Kai Kwok
Youmie Park
Yongsheng Yang
Original Assignee
Univ Michigan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Michigan filed Critical Univ Michigan
Priority to AU60971/00A priority Critical patent/AU6097100A/en
Publication of WO2001004135A2 publication Critical patent/WO2001004135A2/en
Publication of WO2001004135A3 publication Critical patent/WO2001004135A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • A61K47/645—Polycationic or polyanionic oligopeptides, polypeptides or polyamino acids, e.g. polylysine, polyarginine, polyglutamic acid or peptide TAT
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Epidemiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicinal Preparation (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

Disclosed are improved compositions and methods for use in gene delivery and expression. A range of surprisingly effective cross-linking agents and peptides are provided, as are peptide-DNA carrier compositions and condensed particles with reduced toxicity and increased stability. Advantageous methods of using such compositions in gene delivery and gene expression are further disclosed, which may be used in combination with biocompatible matrices, carriers and/or targeting agents.
PCT/US2000/019164 1999-07-13 2000-07-13 Crosslinked dna condensate compositions and gene delivery methods WO2001004135A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AU60971/00A AU6097100A (en) 1999-07-13 2000-07-13 Crosslinked dna condensate compositions and gene delivery methods

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US14360099P 1999-07-13 1999-07-13
US60/143,600 1999-07-13
US15776199P 1999-10-05 1999-10-05
US60/157,761 1999-10-05

Publications (2)

Publication Number Publication Date
WO2001004135A2 WO2001004135A2 (en) 2001-01-18
WO2001004135A3 true WO2001004135A3 (en) 2001-10-04

Family

ID=26841217

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2000/019164 WO2001004135A2 (en) 1999-07-13 2000-07-13 Crosslinked dna condensate compositions and gene delivery methods

Country Status (2)

Country Link
AU (1) AU6097100A (en)
WO (1) WO2001004135A2 (en)

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ES2180416B1 (en) 2001-03-12 2004-06-01 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. PROCEDURE FOR THE PREPARATION OF STABILIZED REACTION MIXTURES, TOTAL OR PARTIALLY DESIRED, THAT INCLUDE, AT LEAST, ONE ENZYME, REACTION MIXES AND KITS CONTAINING THEM.
US7894999B2 (en) 2001-03-27 2011-02-22 Samuel Bogoch Systems and methods for identifying Replikin Scaffolds and uses of said Replikin Scaffolds
KR100906102B1 (en) 2001-03-27 2009-07-07 사무엘 보고치 Replikin peptides and uses thereof
US7420028B2 (en) 2001-03-27 2008-09-02 Samuel Bogoch Replikins and methods of identifying replikin-containing sequences
US7452963B2 (en) 2001-03-27 2008-11-18 Samuel Bogoch Replikin peptides and antibodies therefore
US7774144B2 (en) 2001-10-26 2010-08-10 Samuel Bogoch System and method for identifying complex patterns of amino acids
US7442761B2 (en) * 2003-06-06 2008-10-28 Samuel Bogoch Replikin peptides and uses thereof
US7189800B2 (en) 2001-03-27 2007-03-13 Samuel Bogoch Replikin peptides in rapid replication of glioma cells and in influenza epidemics
SG153654A1 (en) * 2001-07-09 2009-07-29 Samuel Bogoch Replikin peptides and uses thereof
US8494781B2 (en) 2003-06-06 2013-07-23 Samuel Bogoch Systems and methods for identifying replikin scaffolds and uses of said replikin scaffolds
US9254315B2 (en) 2004-04-28 2016-02-09 Samuel Bogoch Systems and methods for identifying replikin scaffolds and uses of said replikin scaffolds
WO2006008096A1 (en) * 2004-07-16 2006-01-26 Micromet Ag Expression-enhanced polypeptides
AU2007280690C1 (en) 2006-07-31 2012-08-23 Curevac Gmbh Nucleic acid of formula (I): GIXmGn, or (II): CIXmCn, in particular as an immune-stimulating agent/adjuvant
EP2092059A4 (en) 2006-10-24 2012-03-28 Bogoch Samuel A method of predicting influenza outbreaks
AU2008266702A1 (en) 2007-05-30 2008-12-24 Elenore S. Bogoch Synthetic replikin peptides against pathogenic infection of invertebrates in aquaculture
WO2009030254A1 (en) 2007-09-04 2009-03-12 Curevac Gmbh Complexes of rna and cationic peptides for transfection and for immunostimulation
EP2176408B9 (en) 2008-01-31 2015-11-11 Curevac GmbH NUCLEIC ACIDS COMPRISING FORMULA (NuGiXmGnNv)a AND DERIVATIVES THEREOF AS AN IMMUNOSTIMULATING AGENTS /ADJUVANTS
WO2010037408A1 (en) 2008-09-30 2010-04-08 Curevac Gmbh Composition comprising a complexed (m)rna and a naked mrna for providing or enhancing an immunostimulatory response in a mammal and uses thereof
WO2010059829A2 (en) * 2008-11-19 2010-05-27 Mdrna, Inc. Compositions and methods for triggered release rna therapeutics
US9233148B2 (en) 2009-01-09 2016-01-12 Samuel Bogoch Replikin-based compounds for prevention and treatment of influenza and methods of differentiating infectivity and lethality in influenza
US20110053829A1 (en) 2009-09-03 2011-03-03 Curevac Gmbh Disulfide-linked polyethyleneglycol/peptide conjugates for the transfection of nucleic acids
KR101761388B1 (en) * 2010-07-30 2017-07-25 큐어백 아게 Complexation of nucleic acids with disulfide-crosslinked cationic components for transfection and immunostimulation
AU2012283914A1 (en) 2011-07-20 2014-01-30 Bogoch, Elanore S. Peptides shared among lethal cancers and therapeutic compositions comprising said peptides
WO2013113326A1 (en) 2012-01-31 2013-08-08 Curevac Gmbh Pharmaceutical composition comprising a polymeric carrier cargo complex and at least one protein or peptide antigen
MX369469B (en) 2013-08-21 2019-11-08 Curevac Ag Respiratory syncytial virus (rsv) vaccine.
EP3129050A2 (en) 2014-04-01 2017-02-15 CureVac AG Polymeric carrier cargo complex for use as an immunostimulating agent or as an adjuvant
WO2018229156A1 (en) 2017-06-14 2018-12-20 Virometix Ag Cyclic peptides for protection against respiratory syncytial virus
CA3121724A1 (en) 2018-12-20 2020-06-25 Virometix Ag Lipopeptide building blocks and synthetic virus-like particles
EP4216992A1 (en) 2020-09-28 2023-08-02 DBV Technologies Particle comprising an rsv-f protein for use in rsv vaccination

Citations (3)

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WO1995002397A1 (en) * 1993-07-14 1995-01-26 The Regents Of The University Of California Self-assembling polynucleotide delivery system comprising dendrimer polycations
WO1998019711A1 (en) * 1996-11-04 1998-05-14 Regents Of The University Of Michigan Peptides for gene delivery
WO1999053961A1 (en) * 1998-04-23 1999-10-28 The Regents Of The University Of Michigan Peptides for efficient gene transfer

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995002397A1 (en) * 1993-07-14 1995-01-26 The Regents Of The University Of California Self-assembling polynucleotide delivery system comprising dendrimer polycations
WO1998019711A1 (en) * 1996-11-04 1998-05-14 Regents Of The University Of Michigan Peptides for gene delivery
WO1999053961A1 (en) * 1998-04-23 1999-10-28 The Regents Of The University Of Michigan Peptides for efficient gene transfer

Non-Patent Citations (11)

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Title
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MERWIN J R ET AL: "TARGETED DELIVERY OF DNA USING YEE(GALNACAH)3, A SYNTHETIC GLYCOPEPTIDE LIGAND FOR THE ASIALOGLYCOPROTEIN RECEPTOR", BIOCONJUGATE CHEMISTRY,US,AMERICAN CHEMICAL SOCIETY, WASHINGTON, vol. 5, no. 6, November 1994 (1994-11-01), pages 612 - 620, XP000484176, ISSN: 1043-1802 *
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WADHWA MANPREET S ET AL: "Glycopeptide carriers for site-targeted, receptor-mediated gene delivery.", PHARMACEUTICAL RESEARCH (NEW YORK), vol. 12, no. 9 (supplement), September 1995 (1995-09-01), & Annual Meeting of the American Association of Pharmaceutical Scientists;Miami Beach, Florida, USA; November 5-9, 1995, pages S79, abstract no. BIOTEC 2002,, XP000990850, ISSN: 0724-8741 *
WADHWA MANPREET S ET AL: "Peptide-mediated gene delivery: Influence of peptide structure on gene expression.", BIOCONJUGATE CHEMISTRY, vol. 8, no. 1, 1997, pages 81 - 88, XP000642731, ISSN: 1043-1802 *
WADHWA MANPREET S ET AL: "Targeted gene delivery with a low molecular weight glycopeptide carrier.", BIOCONJUGATE CHEMISTRY, vol. 6, no. 3, 1995, pages 283 - 291, XP000505485, ISSN: 1043-1802 *

Also Published As

Publication number Publication date
WO2001004135A2 (en) 2001-01-18
AU6097100A (en) 2001-01-30

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